ID   FXL20_HUMAN             Reviewed;         436 AA.
AC   Q96IG2; A8K729; Q38J52;
DT   21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 2.
DT   10-JUN-2026, entry version 184.
DE   RecName: Full=F-box/LRR-repeat protein 20;
DE   AltName: Full=F-box and leucine-rich repeat protein 20;
DE   AltName: Full=F-box/LRR-repeat protein 2-like;
GN   Name=FBXL20; Synonyms=FBL2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA   Stavropoulou A.V., Alao J.P., Lam E.W.F., Coombes R.C., Vigushin D.M.;
RT   "Identification of a novel FBXL20 splice variant.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Colon;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-417, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Platelet;
RX   PubMed=18088087; DOI=10.1021/pr0704130;
RA   Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J.,
RA   Schuetz C., Walter U., Gambaryan S., Sickmann A.;
RT   "Phosphoproteome of resting human platelets.";
RL   J. Proteome Res. 7:526-534(2008).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-417, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA   Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA   Elledge S.J., Gygi S.P.;
RT   "A quantitative atlas of mitotic phosphorylation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-417 AND SER-421, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma, and Erythroleukemia;
RX   PubMed=23186163; DOI=10.1021/pr300630k;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-417, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA   Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT   phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
CC   -!- FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box
CC       protein)-type E3 ubiquitin ligase complex. Role in neural transmission
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with SKP1 and CUL1. {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q96IG2; P63151: PPP2R2A; NbExp=4; IntAct=EBI-8835647, EBI-1048931;
CC       Q96IG2; P63208: SKP1; NbExp=8; IntAct=EBI-8835647, EBI-307486;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q96IG2-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q96IG2-2; Sequence=VSP_030769;
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DR   EMBL; DQ223959; ABB03906.1; -; mRNA.
DR   EMBL; AK291844; BAF84533.1; -; mRNA.
DR   EMBL; BC007557; AAH07557.2; -; mRNA.
DR   CCDS; CCDS32640.1; -. [Q96IG2-1]
DR   CCDS; CCDS54116.1; -. [Q96IG2-2]
DR   RefSeq; NP_001171835.1; NM_001184906.2. [Q96IG2-2]
DR   RefSeq; NP_116264.2; NM_032875.2. [Q96IG2-1]
DR   AlphaFoldDB; Q96IG2; -.
DR   SMR; Q96IG2; -.
DR   BioGRID; 124392; 20.
DR   ComplexPortal; CPX-2873; SCF E3 ubiquitin ligase complex, FBXL20 variant.
DR   CORUM; Q96IG2; -.
DR   FunCoup; Q96IG2; 591.
DR   IntAct; Q96IG2; 9.
DR   NDEx; IQUERY-CP-FBXL20; 1 NDEx IQuery Curated Pathway.
DR   STRING; 9606.ENSP00000264658; -.
DR   TCDB; 8.A.92.1.17; the g-protein AlphaBetaGama complex (gpc) family.
DR   GlyGen; Q96IG2; 1 site.
DR   iPTMnet; Q96IG2; -.
DR   PhosphoSitePlus; Q96IG2; -.
DR   SwissPalm; Q96IG2; -.
DR   BioMuta; FBXL20; -.
DR   DMDM; 38503141; -.
DR   jPOST; Q96IG2; -.
DR   MassIVE; Q96IG2; -.
DR   PaxDb; 9606-ENSP00000264658; -.
DR   PeptideAtlas; Q96IG2; -.
DR   ProteomicsDB; 76826; -. [Q96IG2-1]
DR   ProteomicsDB; 76827; -. [Q96IG2-2]
DR   Pumba; Q96IG2; -.
DR   Antibodypedia; 28248; 151 antibodies from 25 providers.
DR   DNASU; 84961; -.
DR   Ensembl; ENST00000264658.11; ENSP00000264658.6; ENSG00000108306.14. [Q96IG2-1]
DR   Ensembl; ENST00000394294.7; ENSP00000377832.3; ENSG00000108306.14. [Q96IG2-2]
DR   Ensembl; ENST00000583610.5; ENSP00000462271.1; ENSG00000108306.14. [Q96IG2-1]
DR   GeneID; 84961; -.
DR   KEGG; hsa:84961; -.
DR   MANE-Select; ENST00000264658.11; ENSP00000264658.6; NM_032875.3; NP_116264.2.
DR   UCSC; uc002hrt.4; human. [Q96IG2-1]
DR   AGR; HGNC:24679; -.
DR   ClinPGx; PA134976410; -.
DR   CTD; 84961; -.
DR   DisGeNET; 84961; -.
DR   GeneCards; FBXL20; -.
DR   HGNC; HGNC:24679; FBXL20.
DR   HPA; ENSG00000108306; Low tissue specificity.
DR   MIM; 609086; gene.
DR   OpenTargets; ENSG00000108306; -.
DR   VEuPathDB; HostDB:ENSG00000108306; -.
DR   eggNOG; KOG4341; Eukaryota.
DR   GeneTree; ENSGT00940000153845; -.
DR   HOGENOM; CLU_016072_7_1_1; -.
DR   InParanoid; Q96IG2; -.
DR   OMA; LCNRIRY; -.
DR   OrthoDB; 550575at2759; -.
DR   PAN-GO; Q96IG2; 2 GO annotations based on evolutionary models.
DR   PhylomeDB; Q96IG2; -.
DR   PathwayCommons; Q96IG2; -.
DR   Reactome; R-HSA-8951664; Neddylation.
DR   Reactome; R-HSA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   SignaLink; Q96IG2; -.
DR   Agora; ENSG00000108306; -.
DR   BioGRID-ORCS; 84961; 15 hits in 1195 CRISPR screens.
DR   ChiTaRS; FBXL20; human.
DR   GenomeRNAi; 84961; -.
DR   Pharos; Q96IG2; Tbio.
DR   PRO; PR:Q96IG2; -.
DR   Proteomes; UP000005640; Chromosome 17.
DR   RNAct; Q96IG2; protein.
DR   Bgee; ENSG00000108306; Expressed in secondary oocyte and 191 other cell types or tissues.
DR   ExpressionAtlas; Q96IG2; baseline and differential.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0098978; C:glutamatergic synapse; IEA:Ensembl.
DR   GO; GO:0098793; C:presynapse; IEA:GOC.
DR   GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR   GO; GO:0098685; C:Schaffer collateral - CA1 synapse; IEA:Ensembl.
DR   GO; GO:0099575; P:regulation of protein catabolic process at presynapse, modulating synaptic transmission; IEA:Ensembl.
DR   GO; GO:2000300; P:regulation of synaptic vesicle exocytosis; IEA:Ensembl.
DR   GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   CDD; cd22115; F-box_FBXL2-like; 1.
DR   FunFam; 3.80.10.10:FF:000339; F-box/LRR-repeat protein 2 isoform X1; 1.
DR   FunFam; 3.80.10.10:FF:000578; F-box/LRR-repeat protein 2 isoform X1; 1.
DR   FunFam; 3.80.10.10:FF:000042; F-box/LRR-repeat protein 20 isoform 2; 1.
DR   FunFam; 1.20.1280.50:FF:000013; F-box/LRR-repeat protein 20 isoform X1; 1.
DR   Gene3D; 3.80.10.10; Ribonuclease Inhibitor; 2.
DR   InterPro; IPR001810; F-box_dom.
DR   InterPro; IPR057207; FBXL15_LRR.
DR   InterPro; IPR001611; Leu-rich_rpt.
DR   InterPro; IPR006553; Leu-rich_rpt_Cys-con_subtyp.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   PANTHER; PTHR13318:SF47; F-BOX_LRR-REPEAT PROTEIN 20; 1.
DR   PANTHER; PTHR13318; PARTNER OF PAIRED, ISOFORM B-RELATED; 1.
DR   Pfam; PF12937; F-box-like; 1.
DR   Pfam; PF13516; LRR_6; 2.
DR   Pfam; PF25372; LRR_FBXL15; 1.
DR   SMART; SM00256; FBOX; 1.
DR   SMART; SM00367; LRR_CC; 12.
DR   SUPFAM; SSF52047; RNI-like; 1.
DR   PROSITE; PS50181; FBOX; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cytoplasm; Leucine-rich repeat; Phosphoprotein;
KW   Proteomics identification; Reference proteome; Repeat;
KW   Ubl conjugation pathway.
FT   CHAIN           1..436
FT                   /note="F-box/LRR-repeat protein 20"
FT                   /id="PRO_0000119870"
FT   DOMAIN          22..68
FT                   /note="F-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT   REPEAT          74..100
FT                   /note="LRR 1"
FT   REPEAT          101..126
FT                   /note="LRR 2"
FT   REPEAT          127..152
FT                   /note="LRR 3"
FT   REPEAT          153..178
FT                   /note="LRR 4"
FT   REPEAT          179..204
FT                   /note="LRR 5"
FT   REPEAT          205..230
FT                   /note="LRR 6"
FT   REPEAT          231..256
FT                   /note="LRR 7"
FT   REPEAT          257..282
FT                   /note="LRR 8"
FT   REPEAT          283..308
FT                   /note="LRR 9"
FT   REPEAT          309..334
FT                   /note="LRR 10"
FT   REPEAT          335..363
FT                   /note="LRR 11"
FT   REPEAT          364..388
FT                   /note="LRR 12"
FT   REPEAT          389..414
FT                   /note="LRR 13"
FT   MOD_RES         417
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:18088087,
FT                   ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163,
FT                   ECO:0007744|PubMed:24275569"
FT   MOD_RES         421
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   VAR_SEQ         134..165
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_030769"
FT   CONFLICT        17
FT                   /note="S -> P (in Ref. 1; BAF84533)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        375
FT                   /note="L -> F (in Ref. 1; BAF84533)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   436 AA;  48423 MW;  39CD04A505C8CE3E CRC64;
     MRRDVNGVTK SRFEMFSNSD EAVINKKLPK ELLLRIFSFL DVVTLCRCAQ VSRAWNVLAL
     DGSNWQRIDL FDFQRDIEGR VVENISKRCG GFLRKLSLRG CLGVGDNALR TFAQNCRNIE
     VLNLNGCTKT TDATCTSLSK FCSKLRHLDL ASCTSITNMS LKALSEGCPL LEQLNISWCD
     QVTKDGIQAL VRGCGGLKAL FLKGCTQLED EALKYIGAHC PELVTLNLQT CLQITDEGLI
     TICRGCHKLQ SLCASGCSNI TDAILNALGQ NCPRLRILEV ARCSQLTDVG FTTLARNCHE
     LEKMDLEECV QITDSTLIQL SIHCPRLQVL SLSHCELITD DGIRHLGNGA CAHDQLEVIE
     LDNCPLITDA SLEHLKSCHS LERIELYDCQ QITRAGIKRL RTHLPNIKVH AYFAPVTPPP
     SVGGSRQRFC RCCIIL
//