ID   FBXL8_HUMAN             Reviewed;         374 AA.
AC   Q96CD0; Q9NUM0;
DT   07-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   10-JUN-2026, entry version 166.
DE   RecName: Full=F-box/LRR-repeat protein 8;
DE   AltName: Full=F-box and leucine-rich repeat protein 8;
DE   AltName: Full=F-box protein FBL8;
GN   Name=FBXL8; Synonyms=FBL8;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Placenta;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box
CC       protein)-type E3 ubiquitin ligase complex. {ECO:0000250}.
CC   -!- SUBUNIT: Directly interacts with SKP1 and CUL1. {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q96CD0; P13196: ALAS1; NbExp=3; IntAct=EBI-2321097, EBI-3905054;
CC       Q96CD0; Q9NRD5: PICK1; NbExp=3; IntAct=EBI-2321097, EBI-79165;
CC       Q96CD0; P63208: SKP1; NbExp=18; IntAct=EBI-2321097, EBI-307486;
CC   -!- CAUTION: While the gene symbol and protein names are indicative of the
CC       presence of LRR repeats, such repeats are not present in this protein.
CC       {ECO:0000305}.
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DR   EMBL; AK002140; BAA92103.1; -; mRNA.
DR   EMBL; BC014414; AAH14414.1; -; mRNA.
DR   CCDS; CCDS10831.1; -.
DR   RefSeq; NP_060848.2; NM_018378.2.
DR   AlphaFoldDB; Q96CD0; -.
DR   SMR; Q96CD0; -.
DR   BioGRID; 120617; 12.
DR   ComplexPortal; CPX-2438; SCF E3 ubiquitin ligase complex, FBXL8 variant.
DR   FunCoup; Q96CD0; 129.
DR   IntAct; Q96CD0; 8.
DR   STRING; 9606.ENSP00000258200; -.
DR   BioMuta; FBXL8; -.
DR   DMDM; 38257737; -.
DR   jPOST; Q96CD0; -.
DR   MassIVE; Q96CD0; -.
DR   PaxDb; 9606-ENSP00000258200; -.
DR   PeptideAtlas; Q96CD0; -.
DR   ProteomicsDB; 76179; -.
DR   Pumba; Q96CD0; -.
DR   Antibodypedia; 29422; 149 antibodies from 20 providers.
DR   DNASU; 55336; -.
DR   Ensembl; ENST00000258200.8; ENSP00000258200.3; ENSG00000135722.10.
DR   Ensembl; ENST00000519917.5; ENSP00000430490.1; ENSG00000135722.10.
DR   Ensembl; ENST00000959557.1; ENSP00000629616.1; ENSG00000135722.10.
DR   GeneID; 55336; -.
DR   KEGG; hsa:55336; -.
DR   MANE-Select; ENST00000258200.8; ENSP00000258200.3; NM_018378.3; NP_060848.2.
DR   UCSC; uc002erk.2; human.
DR   AGR; HGNC:17875; -.
DR   ClinPGx; PA28028; -.
DR   CTD; 55336; -.
DR   DisGeNET; 55336; -.
DR   GeneCards; FBXL8; -.
DR   HGNC; HGNC:17875; FBXL8.
DR   HPA; ENSG00000135722; Low tissue specificity.
DR   MIM; 609077; gene.
DR   OpenTargets; ENSG00000135722; -.
DR   VEuPathDB; HostDB:ENSG00000135722; -.
DR   eggNOG; ENOG502QU59; Eukaryota.
DR   GeneTree; ENSGT00420000029943; -.
DR   HOGENOM; CLU_062031_1_0_1; -.
DR   InParanoid; Q96CD0; -.
DR   OMA; SCDCERE; -.
DR   OrthoDB; 3219396at2759; -.
DR   PAN-GO; Q96CD0; 2 GO annotations based on evolutionary models.
DR   PhylomeDB; Q96CD0; -.
DR   PathwayCommons; Q96CD0; -.
DR   Reactome; R-HSA-8951664; Neddylation.
DR   Reactome; R-HSA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   SignaLink; Q96CD0; -.
DR   Agora; ENSG00000135722; -.
DR   BioGRID-ORCS; 55336; 8 hits in 1197 CRISPR screens.
DR   GenomeRNAi; 55336; -.
DR   Pharos; Q96CD0; Tdark.
DR   PRO; PR:Q96CD0; -.
DR   Proteomes; UP000005640; Chromosome 16.
DR   RNAct; Q96CD0; protein.
DR   Bgee; ENSG00000135722; Expressed in body of pancreas and 96 other cell types or tissues.
DR   ExpressionAtlas; Q96CD0; baseline and differential.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR   GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   FunFam; 1.20.1280.50:FF:000005; F-box/LRR-repeat protein 3 isoform X1; 1.
DR   FunFam; 3.80.10.10:FF:000260; F-box/LRR-repeat protein 8; 1.
DR   Gene3D; 1.20.1280.50; -; 1.
DR   Gene3D; 3.80.10.10; Ribonuclease Inhibitor; 1.
DR   InterPro; IPR036047; F-box-like_dom_sf.
DR   InterPro; IPR001810; F-box_dom.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   PANTHER; PTHR20872; -; 1.
DR   PANTHER; PTHR20872:SF1; F-BOX DOMAIN-CONTAINING PROTEIN; 1.
DR   Pfam; PF12937; F-box-like; 1.
DR   SMART; SM00256; FBOX; 1.
DR   SUPFAM; SSF81383; F-box domain; 1.
DR   SUPFAM; SSF52047; RNI-like; 1.
DR   PROSITE; PS50181; FBOX; 1.
PE   1: Evidence at protein level;
KW   Proteomics identification; Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..374
FT                   /note="F-box/LRR-repeat protein 8"
FT                   /id="PRO_0000119850"
FT   DOMAIN          2..48
FT                   /note="F-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT   CONFLICT        135
FT                   /note="S -> N (in Ref. 1; BAA92103)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   374 AA;  40516 MW;  7B91444E31E95931 CRC64;
     MAEPGEGLPE EVLALIFRHL SLRDRAAAAR VCRAWAAAAT CSAVWHDTKI SCECELEGML
     PPYLSACLDH IHNLRLEFEP SRKPSRRAAI ELLMVLAGRA PGLRGLRLEC RGEKPLFDAG
     RDVLEAVHAV CGAASQLRHL DLRRLSFTLD DALVLQAARS CPELHSLFLD NSTLVGSVGP
     GSVLELLEAC PRLRALGLHL ASLSHAILEA LAAPDRAPFA LLALRCACPE DARASPLPNE
     AWVALRRRHP GLAVELELEP ALPAESVTRV LQPAVPVAAL RLNLSGDTVG PVRFAAHHYA
     ATLCALEVRA AASAELNAAL EELAARCAAL REVHCFCVVS HSVLDAFRAH CPRLRTYTLK
     LTREPHPWRP TLVA
//