ID   FBX34_HUMAN             Reviewed;         711 AA.
AC   Q9NWN3; Q2VPB5; Q4VBP5; Q86TY4;
DT   12-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 2.
DT   10-JUN-2026, entry version 162.
DE   RecName: Full=F-box only protein 34;
GN   Name=FBXO34; Synonyms=FBX34;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Neuroblastoma;
RA   Li W.B., Gruber C., Jessee J., Polayes D.;
RT   "Full-length cDNA libraries and normalization.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS ASN-470 AND PRO-533.
RC   TISSUE=Placenta;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 405-711, AND VARIANTS ASN-470 AND
RP   PRO-533.
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 407-711, AND VARIANTS ASN-470 AND
RP   PRO-533.
RA   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT   "Cloning of human full open reading frames in Gateway(TM) system entry
RT   vector (pDONR201).";
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   FUNCTION, AND FUNCTION (MICROBIAL INFECTION).
RX   PubMed=36285453; DOI=10.1080/22221751.2022.2140605;
RA   Yang X., Zhao X., Zhu Y., Xun J., Wen Q., Pan H., Yang J., Wang J.,
RA   Liang Z., Shen X., Liang Y., Lin Q., Liang H., Li M., Chen J., Jiang S.,
RA   Xu J., Lu H., Zhu H.;
RT   "FBXO34 promotes latent HIV-1 activation by post-transcriptional
RT   modulation.";
RL   Emerg. Microbes Infect. 11:2785-2799(2022).
CC   -!- FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F-box
CC       protein)-type E3 ubiquitin ligase complex promoting ubiquitination and
CC       proteasomal degradation of specific target proteins including HNRNPU
CC       (PubMed:36285453). Regulates both the G2/M transition and anaphase
CC       entry in meiotic oocytes (By similarity).
CC       {ECO:0000250|UniProtKB:Q80XI1, ECO:0000269|PubMed:36285453}.
CC   -!- FUNCTION: (Microbial infection) Plays a positive role in latent HIV-1
CC       activation. Mechanistically, promotes hnRNP U/HNRNPU ubiquitination,
CC       which leads to its degradation and abolishment of the interaction
CC       between hnRNP U and HIV-1 Rev mRNA. {ECO:0000269|PubMed:36285453}.
CC   -!- SUBUNIT: Directly interacts with SKP1 and CUL1. {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q9NWN3; P05187: ALPP; NbExp=3; IntAct=EBI-719816, EBI-1211484;
CC       Q9NWN3; P27658: COL8A1; NbExp=3; IntAct=EBI-719816, EBI-747133;
CC       Q9NWN3; Q9NRI5-2: DISC1; NbExp=3; IntAct=EBI-719816, EBI-11988027;
CC       Q9NWN3; Q92915-2: FGF14; NbExp=3; IntAct=EBI-719816, EBI-12836320;
CC       Q9NWN3; Q6A162: KRT40; NbExp=3; IntAct=EBI-719816, EBI-10171697;
CC       Q9NWN3; P60412: KRTAP10-11; NbExp=3; IntAct=EBI-719816, EBI-10217483;
CC       Q9NWN3; P60370: KRTAP10-5; NbExp=3; IntAct=EBI-719816, EBI-10172150;
CC       Q9NWN3; P60410: KRTAP10-8; NbExp=3; IntAct=EBI-719816, EBI-10171774;
CC       Q9NWN3; P60329: KRTAP12-4; NbExp=4; IntAct=EBI-719816, EBI-10176396;
CC       Q9NWN3; Q9BYQ6: KRTAP4-11; NbExp=3; IntAct=EBI-719816, EBI-10302392;
CC       Q9NWN3; Q9BQ66: KRTAP4-12; NbExp=3; IntAct=EBI-719816, EBI-739863;
CC       Q9NWN3; Q9BYR2: KRTAP4-5; NbExp=5; IntAct=EBI-719816, EBI-11993254;
CC       Q9NWN3; Q6L8H2: KRTAP5-3; NbExp=3; IntAct=EBI-719816, EBI-11974251;
CC       Q9NWN3; Q9BYQ4: KRTAP9-2; NbExp=3; IntAct=EBI-719816, EBI-1044640;
CC       Q9NWN3; Q9BYQ3: KRTAP9-3; NbExp=3; IntAct=EBI-719816, EBI-1043191;
CC       Q9NWN3; Q9BYQ0: KRTAP9-8; NbExp=3; IntAct=EBI-719816, EBI-11958364;
CC       Q9NWN3; Q99750: MDFI; NbExp=3; IntAct=EBI-719816, EBI-724076;
CC       Q9NWN3; Q5JR59: MTUS2; NbExp=3; IntAct=EBI-719816, EBI-742948;
CC       Q9NWN3; Q5JR59-3: MTUS2; NbExp=4; IntAct=EBI-719816, EBI-11522433;
CC       Q9NWN3; Q8WWZ8: OIT3; NbExp=3; IntAct=EBI-719816, EBI-10277776;
CC       Q9NWN3; B2RUY7: VWC2L; NbExp=3; IntAct=EBI-719816, EBI-11747707;
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA91346.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=CAD62596.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BX248268; CAD62596.1; ALT_INIT; mRNA.
DR   EMBL; BC095482; AAH95482.2; -; mRNA.
DR   EMBL; BC109120; AAI09121.1; -; mRNA.
DR   EMBL; BC109121; AAI09122.1; -; mRNA.
DR   EMBL; AK000732; BAA91346.1; ALT_INIT; mRNA.
DR   EMBL; CR457249; CAG33530.1; -; mRNA.
DR   CCDS; CCDS32086.1; -.
DR   RefSeq; NP_060413.2; NM_017943.3.
DR   RefSeq; NP_689417.1; NM_152231.2.
DR   RefSeq; XP_006720248.1; XM_006720185.4.
DR   RefSeq; XP_016876882.1; XM_017021393.3.
DR   RefSeq; XP_054232257.1; XM_054376282.1.
DR   RefSeq; XP_054232258.1; XM_054376283.1.
DR   AlphaFoldDB; Q9NWN3; -.
DR   BioGRID; 120359; 58.
DR   ComplexPortal; CPX-7975; SCF E3 ubiquitin ligase complex, FBXO34 variant.
DR   FunCoup; Q9NWN3; 634.
DR   IntAct; Q9NWN3; 35.
DR   STRING; 9606.ENSP00000313159; -.
DR   iPTMnet; Q9NWN3; -.
DR   PhosphoSitePlus; Q9NWN3; -.
DR   BioMuta; FBXO34; -.
DR   DMDM; 61252689; -.
DR   MassIVE; Q9NWN3; -.
DR   PaxDb; 9606-ENSP00000313159; -.
DR   PeptideAtlas; Q9NWN3; -.
DR   ProteomicsDB; 82956; -.
DR   Antibodypedia; 24028; 99 antibodies from 17 providers.
DR   DNASU; 55030; -.
DR   Ensembl; ENST00000313833.5; ENSP00000313159.4; ENSG00000178974.12.
DR   Ensembl; ENST00000440021.1; ENSP00000394117.1; ENSG00000178974.12.
DR   Ensembl; ENST00000679934.1; ENSP00000505918.1; ENSG00000178974.12.
DR   Ensembl; ENST00000680658.1; ENSP00000506499.1; ENSG00000178974.12.
DR   Ensembl; ENST00000680682.1; ENSP00000504946.1; ENSG00000178974.12.
DR   Ensembl; ENST00000681074.1; ENSP00000506304.1; ENSG00000178974.12.
DR   Ensembl; ENST00000681400.1; ENSP00000506349.1; ENSG00000178974.12.
DR   Ensembl; ENST00000681904.1; ENSP00000505815.1; ENSG00000178974.12.
DR   Ensembl; ENST00000855357.1; ENSP00000525416.1; ENSG00000178974.12.
DR   Ensembl; ENST00000855358.1; ENSP00000525417.1; ENSG00000178974.12.
DR   Ensembl; ENST00000855359.1; ENSP00000525418.1; ENSG00000178974.12.
DR   Ensembl; ENST00000855360.1; ENSP00000525419.1; ENSG00000178974.12.
DR   Ensembl; ENST00000855361.1; ENSP00000525420.1; ENSG00000178974.12.
DR   Ensembl; ENST00000918751.1; ENSP00000588810.1; ENSG00000178974.12.
DR   Ensembl; ENST00000918752.1; ENSP00000588811.1; ENSG00000178974.12.
DR   Ensembl; ENST00000969173.1; ENSP00000639232.1; ENSG00000178974.12.
DR   Ensembl; ENST00000969174.1; ENSP00000639233.1; ENSG00000178974.12.
DR   GeneID; 55030; -.
DR   KEGG; hsa:55030; -.
DR   MANE-Select; ENST00000313833.5; ENSP00000313159.4; NM_017943.4; NP_060413.2.
DR   UCSC; uc001xbu.4; human.
DR   AGR; HGNC:20201; -.
DR   ClinPGx; PA134910884; -.
DR   CTD; 55030; -.
DR   DisGeNET; 55030; -.
DR   GeneCards; FBXO34; -.
DR   HGNC; HGNC:20201; FBXO34.
DR   HPA; ENSG00000178974; Low tissue specificity.
DR   MIM; 609104; gene.
DR   OpenTargets; ENSG00000178974; -.
DR   VEuPathDB; HostDB:ENSG00000178974; -.
DR   eggNOG; ENOG502QRQQ; Eukaryota.
DR   GeneTree; ENSGT00530000064222; -.
DR   InParanoid; Q9NWN3; -.
DR   OMA; MKNSNRY; -.
DR   OrthoDB; 10052741at2759; -.
DR   PAN-GO; Q9NWN3; 0 GO annotations based on evolutionary models.
DR   PhylomeDB; Q9NWN3; -.
DR   PathwayCommons; Q9NWN3; -.
DR   SignaLink; Q9NWN3; -.
DR   Agora; ENSG00000178974; -.
DR   BioGRID-ORCS; 55030; 10 hits in 1198 CRISPR screens.
DR   ChiTaRS; FBXO34; human.
DR   GenomeRNAi; 55030; -.
DR   Pharos; Q9NWN3; Tdark.
DR   PRO; PR:Q9NWN3; -.
DR   Proteomes; UP000005640; Chromosome 14.
DR   RNAct; Q9NWN3; protein.
DR   Bgee; ENSG00000178974; Expressed in secondary oocyte and 212 other cell types or tissues.
DR   ExpressionAtlas; Q9NWN3; baseline and differential.
DR   GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; NAS:ComplexPortal.
DR   CDD; cd22176; F-box_FBXO34; 1.
DR   InterPro; IPR036047; F-box-like_dom_sf.
DR   InterPro; IPR001810; F-box_dom.
DR   InterPro; IPR039594; FBXO34/46.
DR   PANTHER; PTHR16271:SF11; F-BOX ONLY PROTEIN 34; 1.
DR   PANTHER; PTHR16271; F-BOX ONLY PROTEIN 34/46 FAMILY MEMBER; 1.
DR   Pfam; PF12937; F-box-like; 1.
DR   SUPFAM; SSF81383; F-box domain; 1.
DR   PROSITE; PS50181; FBOX; 1.
PE   1: Evidence at protein level;
KW   Proteomics identification; Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..711
FT                   /note="F-box only protein 34"
FT                   /id="PRO_0000119926"
FT   DOMAIN          572..624
FT                   /note="F-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT   REGION          1..36
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          249..271
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          337..372
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          494..529
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        10..23
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        354..364
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         432
FT                   /note="V -> A (in dbSNP:rs35070799)"
FT                   /id="VAR_049047"
FT   VARIANT         470
FT                   /note="I -> N (in dbSNP:rs1045002)"
FT                   /evidence="ECO:0000269|PubMed:14702039,
FT                   ECO:0000269|PubMed:15489334, ECO:0000269|Ref.4"
FT                   /id="VAR_021489"
FT   VARIANT         533
FT                   /note="L -> P (in dbSNP:rs3742569)"
FT                   /evidence="ECO:0000269|PubMed:14702039,
FT                   ECO:0000269|PubMed:15489334, ECO:0000269|Ref.4"
FT                   /id="VAR_021490"
FT   VARIANT         704
FT                   /note="G -> V (in dbSNP:rs10138395)"
FT                   /id="VAR_049048"
SQ   SEQUENCE   711 AA;  78711 MW;  6D289A9320AE9C42 CRC64;
     MHLKPYWKLQ KKEHPPEVSR ETQRTPMNHQ KAVNDETCKA SHITSSVFPS ASLGKASSRK
     PFGILSPNVL CSMSGKSPVE SSLNVKTKKN APSATIHQGE EEGPLDIWAV VKPGNTKEKI
     AFFASHQCSN RIGSMKIKSS WDIDGRATKR RKKSGDLKKA KVQVERMREV NSRCYQPEPF
     ACGIEHCSVH YVSDSGDGVY AGRPLSVIQM VAFLEQRASA LLASCSKNCT NSPAIVRFSG
     QSRGVPAVSE SYSAPGACEE PTERGNLEVG EPQSEPVRVL DMVAKLESEC LKRQGQREPG
     SLSRNNSFRR NVGRVLLANS TQADEGKTKK GVLEAPDTQV NPVGSVSVDC GPSRADRCSP
     KEDQAWDGAS QDCPPLPAGV SFHIDSAELE PGSQTAVKNS NRYDVEMTDE LVGLPFSSHT
     YSQASELPTD AVDCMSRELV SLTSRNPDQR KESLCISITV SKVDKDQPSI LNSCEDPVPG
     MLFFLPPGQH LSDYSQLNES TTKESSEASQ LEDAAGGDSA SEEKSGSAEP FVLPASSVES
     TLPVLEASSW KKQVSHDFLE TRFKIQQLLE PQQYMAFLPH HIMVKIFRLL PTKSLVALKC
     TCCYFKFIIE YYNIRPADSR WVRDPRYRED PCKQCKKKYV KGDVSLCRWH PKPYCQALPY
     GPGYWMCCHR SQKGFPGCKL GLHDNHWVPA CHSFNRAIHK KAKGTEAEEE Y
//