| Evidence type | Biological context (meiosis stage/cell type) | Molecular function claim | Key partners/substrates | Subcellular localization | Experimental approach | Quantitative/statistical notes | Species (mouse/human cells) | Citation (include DOI URL + year) |
|---|---|---|---|---|---|---|---|---|
| Primary | Early meiotic prophase I spermatocytes; bouquet/telomere attachment stage | FBXO47 is an F-box/SCF-associated factor required for telomere-inner nuclear envelope integration and stabilizes TRF2 rather than promoting its degradation | SKP1; TRF1; TRF2 | Nuclear periphery; telomere-associated sites at the inner nuclear membrane | CRISPR FLAG knock-in, co-immunoprecipitation, HEK293T co-expression, ubiquitination assays, cycloheximide chase, IF on chromosome spreads, EM/FIB-SEM | n > 30 cells/genotype for internal TRF1 foci; P < 0.001; TRF1 intensity not decreased (8.769 ± 0.351 vs 9.786 ± 0.6768), while TRF2 intensity decreased in knockout spermatocytes (leptotene 23.873 ± 5.414 vs 19.660 ± 4.207; zygotene 38.046 ± 7.281 vs 18.953 ± 13.110) | Mouse testes/spermatocytes; human HEK293T cells | Hua et al., Nucleic Acids Research, 2019, https://doi.org/10.1093/nar/gkz992 (pqac-00000001, pqac-00000002, pqac-00000003, pqac-00000004, pqac-00000005, pqac-00000007, pqac-00000010) |
| Primary | Early meiotic recombination in spermatocytes | FBXO47 acts as a meiosis-specific F-box protein interacting with SKP1 and HORMAD1 and can target HORMAD1 for polyubiquitination/degradation, linking SCF activity to control of meiotic DSB homeostasis | SKP1; HORMAD1 | Meiotic chromosomal axis context inferred from HORMAD1 biology; exact FBXO47 localization not the main focus in extracted evidence | Mouse meiotic genetics plus HEK293T interaction/ubiquitination assays | No specific numeric effect sizes extracted here for FBXO47 itself; study supports mechanism that SCF restrains hyperactive DSB formation through HORMAD1 turnover | Mouse germ cells; human HEK293T cells | Guan et al., Nucleic Acids Research, 2022, https://doi.org/10.1093/nar/gkac304 (pqac-00000007) |
| Primary | Zygotene-to-pachytene spermatocytes; centromere pairing and pachynema progression | FBXO47 is proposed as a centromeric SCF E3 ligase component that stabilizes SKP1 by suppressing its ubiquitination and thereby promotes centromere pairing, synapsis integrity, and meiotic progression | SKP1; HORMAD1 (regulatory connection proposed) | Centromeres; chromosome axes; study also notes effects on telomere-NE attachment | Co-IP in HEK293T, ubiquitination assays, chromosome spreads, IF localization across meiotic stages | Reported centromeric-end/counterpart SKP1 signal ratio 3.62; figure-level significance includes p = 0.0003, p = 0.0016, p < 0.0001; knockout causes leptotene/zygotene delay, pachytene-like arrest, crossover failure | Mouse spermatocytes; human HEK293T cells | Ma et al., Communications Biology, 2024, https://doi.org/10.1038/s42003-024-06782-6 (pqac-00000006, pqac-00000008, pqac-00000012, pqac-00000013) |
| Review | Spermatogenesis, especially meiotic prophase I in spermatocytes | Expert synthesis: FBXO47 is a meiotic F-box protein essential for telomere-NE integration, homologous chromosome synapsis, bouquet-stage progression, and male fertility; current literature supports a telomere-centric role, though newer work introduces centromere-focused models | TRF1; TRF2; broader SCF machinery | Nuclear surface/telomere-NE interface during meiosis | Narrative review of primary literature in mouse and comparative systems | Summarizes phenotypes such as reduced testis weight, abnormal spermatocyte-like cells, late-zygotene arrest, autosomal gamma-H2AX retention, and absence of XY bodies; no new original quantitative dataset | Review of mouse and other model-organism data | Xuan et al., Cell Regeneration, 2024, https://doi.org/10.1186/s13619-024-00196-9 (pqac-00000014, pqac-00000015) |
| Database | Human disease association landscape | Human FBXO47 is associated most strongly with azoospermia in Open Targets, consistent with meiosis/spermatogenesis biology; other weaker associations are present but less biologically specific | Disease association evidence linked to literature rather than direct substrate data | Not applicable | Integrated disease-target evidence aggregation | Open Targets evidence score for azoospermia association 0.1046948485 with 5 evidence items; additional lower-confidence associations include hypertension, hypothyroidism, facial pain, and partial chromosome Y deletion | Human | Open Targets Platform, FBXO47 target page, https://platform.opentargets.org/target/ENSG00000204952 (pqac-00000000) |


*Table: This table summarizes the main functional annotation evidence for human FBXO47 by integrating primary mechanistic studies, a recent review, and a disease-association database. It highlights the current consensus and controversy around FBXO47's meiotic role, binding partners, localization, and links to male infertility.*