FGG encodes the gamma subunit of fibrinogen, a secreted complex containing two alpha, two beta and two gamma chains. Gamma interfaces contribute to assembly and, after thrombin acts on the other chains, recognize exposed fibrin knobs to support the extracellular clot network. Gamma-containing fibrinogen also bridges activated platelets. Receptor-recognition regions contribute to contextual endothelial adhesion and signaling. Alternative C termini distinguish gamma-A from the longer gamma-B (gamma-prime) product; experimental fragments, multimerized preparations and clinical splice mutants do not establish equal activity of these natural products. Pathogenic FGG variants can impair assembly, secretion or clot properties, producing quantitative or qualitative fibrinogen disorders.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005102 signaling receptor binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: Gamma contributes an ICAM-1-recognition region in fibrinogen. Reason: PMID7822297 explicitly maps receptor recognition to gamma117–133 in its abstract. Independently read PMID10903502 adhesion/survival experiments use this peptide and ICAM-1 controls. The same molecular claim is supported, without inferring the contact site from the InterPro match alone or making isolated gamma a general growth factor. Complete PMID7822297 Methods were unavailable. GO:0005102 and GO:0050839 are not ancestors of one another in the saved typed ontology. The former records recognition of a signaling receptor, supported by the ICAM-1/ERK context; the latter records the adhesion-molecule class. Both are non-core, with no duplicate core function. Supporting Evidence: GO_REF:0000002 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005102 signaling receptor binding | IPI PMID:7822297 Structural recognition of a novel fibrinogen gamma chain seq... | KEEP AS NON CORE | Summary: Gamma contributes an ICAM-1-recognition region in fibrinogen. Reason: PMID7822297 explicitly maps receptor recognition to gamma117–133 in its abstract. Independently read PMID10903502 adhesion/survival experiments use this peptide and ICAM-1 controls. The same molecular claim is supported, without inferring the contact site from the InterPro match alone or making isolated gamma a general growth factor. Complete PMID7822297 Methods were unavailable. GO:0005102 and GO:0050839 are not ancestors of one another in the saved typed ontology. The former records recognition of a signaling receptor, supported by the ICAM-1/ERK context; the latter records the adhesion-molecule class. Both are non-core, with no duplicate core function. |
| GO:0005198 structural molecule activity | IDA PMID:8910396 The role of betagamma and alphagamma complexes in the assemb... | ACCEPT | Summary: Gamma supplies structural interfaces for fibrinogen assembly and fibrin formation. Reason: Actual PMID8910396 Results follow human alpha-gamma and beta-gamma assembly intermediates in BHK recipients. Human gamma-fragment binding and structure independently establish polymerization contacts. Structural molecule activity covers complex assembly as well as the extracellular polymer; gamma is neither thrombin nor the factor XIII crosslinking enzyme. |
| GO:0005201 extracellular matrix structural constituent | HDA PMID:28344315 Proteomic characterization of human multiple myeloma bone ma... | UNDECIDED | Summary: FGG has a supported matrix structural role; the source-specific extension remains unverified. Reason: Human gamma-fragment/pocket work establishes fibrin structural participation, and independent FGG proteomic joins support matrix association. The generic GO:0005201 claim is therefore supported and remains non-core in the other rows. This raw annotation additionally asserts occurs_in(UBERON:0002371); its exact specimen-specific FGG table was not recovered. UNDECIDED applies to that complete extended assertion, not to the established generic function. The original extension is restored as source metadata; no absence or wrong-gene inference is made. Supporting Evidence: file:human/FGG/FGG-source-provenance.yaml |
| GO:0005201 extracellular matrix structural constituent | RCA PMID:25037231 Extracellular matrix signatures of human primary metastatic ... | KEEP AS NON CORE | Summary: FGG is detected in matrix preparations and contributes to fibrin structure. Reason: The exact FGG target is present in the original supplement. Matrisome classification alone is not a mechanical assay; independent human gamma-fragment/pocket work supports fibrin structural participation. Retain the matrix-specific annotation without duplicating the broader structural core, which also covers fibrinogen assembly. Supporting Evidence: file:human/FGG/FGG-source-provenance.yaml |
| GO:0005201 extracellular matrix structural constituent | RCA PMID:28327460 Comprehensive proteomic characterization of stem cell-derive... | UNDECIDED | Summary: FGG has a supported matrix structural role; the source-specific extension remains unverified. Reason: Human gamma-fragment/pocket work establishes fibrin structural participation, and independent FGG proteomic joins support matrix association. The generic GO:0005201 claim is therefore supported and remains non-core in the other rows. This raw annotation additionally asserts occurs_in(CL:0002540); its exact specimen-specific FGG table was not recovered. UNDECIDED applies to that complete extended assertion, not to the established generic function. The original extension is restored as source metadata; no absence or wrong-gene inference is made. Supporting Evidence: file:human/FGG/FGG-source-provenance.yaml |
| GO:0005201 extracellular matrix structural constituent | RCA PMID:28675934 Characterization of the Extracellular Matrix of Normal and D... | KEEP AS NON CORE | Summary: FGG is detected in matrix preparations and contributes to fibrin structure. Reason: The exact FGG target is present in the original supplement. Matrisome classification alone is not a mechanical assay; independent human gamma-fragment/pocket work supports fibrin structural participation. Retain the matrix-specific annotation without duplicating the broader structural core, which also covers fibrinogen assembly. Supporting Evidence: file:human/FGG/FGG-source-provenance.yaml |
| GO:0005515 protein binding | IPI PMID:10788510 The fibronectin-binding MSCRAMM FnbpA of Staphylococcus aure... | MODIFY | Summary: Gamma binds bacterial cell-surface adhesion molecules. Reason: The PMID:10788510 abstract identifies the tested MSCRAMMs as microbial surface proteins mediating adherence to host matrix. It directly localizes recombinant FnBPA recognition to the gamma C terminus, reports binding measurements and competition with ClfA. GO:0050839 describes binding to a cell adhesion molecule, so it captures the verified partner class more specifically than generic protein binding. This assigns the binding activity to FGG, not microbial adhesin activity. Complete strain/construct Methods and accession-specific mapping were not recovered; the original IPI partner identifiers are preserved with curator deference. No natural-isoform specificity is inferred from the peptide. Proposed replacements: cell adhesion molecule binding Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:10788510 The fibronectin-binding MSCRAMM FnbpA of Staphylococcus aure... | MODIFY | Summary: Gamma binds bacterial cell-surface adhesion molecules. Reason: The PMID:10788510 abstract identifies the tested MSCRAMMs as microbial surface proteins mediating adherence to host matrix. It directly localizes recombinant FnBPA recognition to the gamma C terminus, reports binding measurements and competition with ClfA. GO:0050839 describes binding to a cell adhesion molecule, so it captures the verified partner class more specifically than generic protein binding. This assigns the binding activity to FGG, not microbial adhesin activity. Complete strain/construct Methods and accession-specific mapping were not recovered; the original IPI partner identifiers are preserved with curator deference. No natural-isoform specificity is inferred from the peptide. Proposed replacements: cell adhesion molecule binding Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:21546586 Role of Mycoplasma pneumoniae glyceraldehyde-3-phosphate deh... | UNDECIDED | Summary: The gamma-chain assignment in the GAPDH-binding experiment remains unverified. Reason: The abstract reports microbial GAPDH binding to human fibrinogen in a Western-blot assay but does not resolve the FGG band or construct. The relevant original result was unavailable. Whole-fibrinogen recognition is positive context, not proof of isolated gamma binding. Supporting Evidence: |
| GO:0005515 protein binding | IPI PMID:26627825 Extracellular Fibrinogen-binding Protein (Efb) from Staphylo... | KEEP AS NON CORE | Summary: FGG is recovered in the Efb affinity-capture experiment. Reason: Actual Results and Table 1 identify FIBG_HUMAN in platelet-lysate capture with added fibrinogen, at enrichment 1.40 relative to bead control. This supports association in the tested mixture, not purified gamma affinity or transfer of the microbial protein activities to FGG. No more informative host MF is established by this capture alone. Supporting Evidence: |
| GO:0005576 extracellular region | EXP PMID:10074346 Conformational changes in fragments D and double-D from huma... | ACCEPT | Summary: Human fibrin(ogen) fragments contain extracellular gamma chains. Reason: The source abstracts describe human D/double-D structures. The complete originals were unavailable, but independently read human plasma structure and human-chain assembly/secretion experiments establish the identical generic extracellular location. No unexamined source-specific conformational result is asserted. |
| GO:0005576 extracellular region | EXP PMID:19296670 Crystal structure of human fibrinogen. | ACCEPT | Summary: Human plasma fibrinogen contains the extracellular gamma subunit. Reason: Actual Methods purify human plasma fibrinogen and the structure resolves gamma-containing D regions and coiled coils. Partial alpha-chain proteolysis and minor fibrinopeptide cleavage limit claims of a wholly intact native preparation, not the extracellular location. Supporting Evidence: |
| GO:0005576 extracellular region | EXP PMID:9628725 Crystal structure of fragment double-D from human fibrin wit... | ACCEPT | Summary: Human fibrin(ogen) fragments contain extracellular gamma chains. Reason: The source abstracts describe human D/double-D structures. The complete originals were unavailable, but independently read human plasma structure and human-chain assembly/secretion experiments establish the identical generic extracellular location. No unexamined source-specific conformational result is asserted. |
| GO:0005576 extracellular region | HDA PMID:16502470 Human colostrum: identification of minor proteins in the aqu... | UNDECIDED | Summary: Extracellular FGG is established; the colostrum-specific source assertion remains unverified. Reason: Human plasma structure and chain assembly/secretion experiments establish the generic extracellular location. This raw HDA annotation additionally asserts part_of(UBERON:0001914) in the colostrum survey, whose exact FGG target table remains inaccessible. UNDECIDED concerns that complete specimen-specific assertion, not whether FGG can occur extracellularly. The original extension is restored as source metadata; another extracellular preparation does not independently verify the colostrum assignment. Supporting Evidence: file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | IDA PMID:6777381 Ca2+-mediated association of glycoprotein G (thrombinsensiti... | ACCEPT | Summary: Released platelet fibrin(ogen) is extracellular. Reason: Actual Results distinguish soluble release from calcium-dependent surface retention and identify fibrin-chain bands in human platelets. This is complex-level evidence, not a membrane-spanning gamma protein or an isoform-resolved assay. Supporting Evidence: |
| GO:0005576 extracellular region | IEA GO_REF:0000044 | ACCEPT | Summary: FGG is a secreted component of extracellular fibrinogen. Reason: The literal UniProt Secreted location and mapping agree with human plasma purification and human-chain assembly/secretion experiments. Precursor processing does not imply identical activity of every splice form. |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-114560 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-114560 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-114560 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-140840 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-140840 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-140840 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-140842 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-140842 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-140842 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-140851 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-140851 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-140851 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-166072 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-166072 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-166072 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-166082 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-166082 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-166082 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-166091 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-166091 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-166091 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-166119 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-166119 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-166119 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-166284 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-166284 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-166284 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-166286 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-166286 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-166286 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-166362 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-166362 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-166362 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-166363 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-166363 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-166363 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-216069 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-216069 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-216069 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-216082 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-216082 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-216082 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-2201316 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-2201316 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-2201316 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-2201322 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-2201322 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-2201322 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-2262775 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-2262775 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-2262775 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-2262777 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-2262777 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-2262777 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-2559414 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-2559414 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-2559414 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-354066 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-354066 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-354066 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-354073 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-354073 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-354073 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-354087 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-354087 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-354087 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-354124 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-354124 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-354124 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-354149 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-354149 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-354149 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-354165 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-354165 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-354165 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-372693 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-372693 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-372693 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-372697 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-372697 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-372697 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-372705 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-372705 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-372705 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-377640 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-377640 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-377640 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-377641 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-377641 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-377641 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-377643 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-377643 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-377643 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-377644 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-377644 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-377644 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-429415 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-429415 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-429415 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-429441 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-429441 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-429441 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-481007 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-481007 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-481007 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-5602353 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-5602353 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-5602353 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-5602383 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-5602383 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-5602383 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-5602606 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-5602606 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-5602606 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-8870678 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-8870678 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-8870678 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-937022 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-937022 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-937022 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-937059 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-937059 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-937059 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-937079 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-937079 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-937079 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-9936699 | ACCEPT | Summary: FGG is modeled in the extracellular region component of this event. Reason: The actual Reactome:R-HSA-9936699 component route reaches human P02679 entity 140585, modeled residues 27–453, in extracellular region. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Supporting Evidence: Reactome:R-HSA-9936699 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005577 fibrinogen complex | IDA PMID:16846481 The fibrinogen Aalpha R16C mutation results in fibrinolytic ... | ACCEPT | Summary: Gamma is a constituent of the fibrinogen complex. Reason: The source abstracts describe purified/expressed human fibrinogen, including gamma variants where relevant. Complete source-specific assays were unavailable. Independent human-chain assembly and plasma structure establish this identical generic membership claim; variant mechanisms or natural-splice activity are not inferred from membership. |
| GO:0005577 fibrinogen complex | IDA PMID:18676163 Congenital hypofibrinogenemia: characterization of two misse... | ACCEPT | Summary: Gamma is a constituent of the fibrinogen complex. Reason: The source abstracts describe purified/expressed human fibrinogen, including gamma variants where relevant. Complete source-specific assays were unavailable. Independent human-chain assembly and plasma structure establish this identical generic membership claim; variant mechanisms or natural-splice activity are not inferred from membership. |
| GO:0005577 fibrinogen complex | IDA PMID:6451630 Localization of a fibrin polymerization site. | ACCEPT | Summary: A gamma-derived polymerization fragment is isolated from human fibrinogen. Reason: The actual fragment-D study identifies a gamma-derived peptide and its recognition of fibrin-related fragments. This establishes complex membership; the experimental fragment is not a natural splice product. Supporting Evidence: |
| GO:0005577 fibrinogen complex | IDA PMID:8470043 Paris I dysfibrinogenemia: a point mutation in intron 8 resu... | ACCEPT | Summary: Gamma is a constituent of the fibrinogen complex. Reason: The source abstracts describe purified/expressed human fibrinogen, including gamma variants where relevant. Complete source-specific assays were unavailable. Independent human-chain assembly and plasma structure establish this identical generic membership claim; variant mechanisms or natural-splice activity are not inferred from membership. |
| GO:0005577 fibrinogen complex | IDA PMID:8910396 The role of betagamma and alphagamma complexes in the assemb... | ACCEPT | Summary: Gamma-containing intermediates assemble into human fibrinogen. Reason: Actual Results follow alpha-gamma/beta-gamma intermediates and full assembly after supplying the missing chain. The human-chain fusion experiments use hamster BHK recipients; human hepatocyte-derived observations are a separate context. Supporting Evidence: |
| GO:0005577 fibrinogen complex | IEA GO_REF:0000120 | ACCEPT | Summary: Electronic complex membership agrees with human assembly evidence. Reason: The actual ARBA00086903 FunFam/Primates branch and InterPro IPR012290 target mapping were verified. Human gamma-containing intermediates and plasma fibrinogen independently corroborate membership; the mappings are not new experiments. Supporting Evidence: GO_REF:0000120 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005788 endoplasmic reticulum lumen | TAS Reactome:R-HSA-8952289 | KEEP AS NON CORE | Summary: FGG is modeled in the endoplasmic reticulum lumen component of this event. Reason: The actual Reactome:R-HSA-8952289 component route reaches human P02679 entity 8956692, 8956993, modeled residues 27–453, in endoplasmic reticulum lumen. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. FGG is modeled as a FAM20C substrate, not the kinase. Supporting Evidence: Reactome:R-HSA-8952289 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-114560 | KEEP AS NON CORE | Summary: FGG is modeled in the plasma membrane component of this event. Reason: The actual Reactome:R-HSA-114560 component route reaches human P02679 entity 140918, modeled residues 27–453, in plasma membrane. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Plasma membrane here denotes extracellularly associated fibrin in an integrin complex, not a transmembrane gamma chain. Supporting Evidence: Reactome:R-HSA-114560 file:human/FGG/FGG-source-provenance.yaml |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-9921091 | KEEP AS NON CORE | Summary: FGG is modeled in the plasma membrane component of this event. Reason: The actual Reactome:R-HSA-9921091 component route reaches human P02679 entity 140918, modeled residues 27–453, in plasma membrane. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. Plasma membrane here denotes extracellularly associated fibrin in an integrin complex, not a transmembrane gamma chain. Supporting Evidence: Reactome:R-HSA-9921091 file:human/FGG/FGG-source-provenance.yaml |
| GO:0007160 cell-matrix adhesion | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Cell-matrix adhesion is a positive inherited and contextual gamma role. Reason: The positive IBD at PTN009036378 is on the target path to PTN002499052; the MSA exactly matches canonical human FGG. Self-WITH is legitimate descendant evidence. Gamma-region/ICAM-1 substrate-adhesion assays corroborate the contextual role, retained outside the principal hemostatic core. |
| GO:0007160 cell-matrix adhesion | IDA PMID:10903502 Fibrinogen interactions with ICAM-1 (CD54) regulate endothel... | KEEP AS NON CORE | Summary: Fibrinogen and its gamma-region peptide support endothelial adhesion. Reason: Actual adhesion Methods, Table 1 and Results use fibrinogen substrates and gamma117–133 with ICAM-1 controls. This supports the tested matrix-adhesion context, not autonomous gamma activity in every tissue or exclusive ICAM-1 use. Supporting Evidence: |
| GO:0007596 blood coagulation | IEA GO_REF:0000002 | TOO GENERAL | Summary: The positive broad hemostatic mapping is less informative than retained specific processes. Reason: Actual typed GO ancestry places platelet aggregation below platelet activation/blood coagulation and fibrin clot formation below blood coagulation. Gamma bridging and pocket evidence support the retained specific processes. This is ontology refinement, not rejection of hemostatic participation or assignment of protease activity. Supporting Evidence: GO_REF:0000002 file:human/FGG/FGG-source-provenance.yaml |
| GO:0009306 protein secretion | IMP PMID:18676163 Congenital hypofibrinogenemia: characterization of two misse... | UNDECIDED | Summary: The secretion-specific interpretation of the gamma variant remains unresolved. Reason: The abstract explicitly reports gammaAsn345Ser impairing hexamer secretion, distinct from alphaCys45Phe assembly failure. Full expression experiments were unavailable. A defect in assembly/quality control of secreted cargo does not by itself distinguish direct participation in secretion. Preserve the IMP assertion pending the relevant full result. Supporting Evidence: |
| GO:0009897 external side of plasma membrane | IDA PMID:6777381 Ca2+-mediated association of glycoprotein G (thrombinsensiti... | KEEP AS NON CORE | Summary: Fibrin-chain material associates with activated human platelet surfaces. Reason: Actual surface-iodination Results and Figure 3 caption identify fibrin-chain bands after activation, with calcium-dependent extracellular retention. This supports complex surface association, not gamma membrane insertion or isoform-specific topology. Supporting Evidence: |
| GO:0009897 external side of plasma membrane | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: The external platelet-surface mapping has matching human evidence. Reason: ARBA00043397 matches FGG FunFam/Haplorrhini. Human surface iodination and calcium-dependent fibrin retention support gamma as a subunit of the extracellularly bound complex. |
| GO:0009986 cell surface | IDA PMID:6777381 Ca2+-mediated association of glycoprotein G (thrombinsensiti... | KEEP AS NON CORE | Summary: Fibrin-chain material associates with activated human platelet surfaces. Reason: Actual surface-iodination Results and Figure 3 caption identify fibrin-chain bands after activation, with calcium-dependent extracellular retention. This supports complex surface association, not gamma membrane insertion or isoform-specific topology. Supporting Evidence: |
| GO:0030168 platelet activation | IEA GO_REF:0000002 | TOO GENERAL | Summary: The positive broad hemostatic mapping is less informative than retained specific processes. Reason: Actual typed GO ancestry places platelet aggregation below platelet activation/blood coagulation and fibrin clot formation below blood coagulation. Gamma bridging and pocket evidence support the retained specific processes. This is ontology refinement, not rejection of hemostatic participation or assignment of protease activity. Supporting Evidence: GO_REF:0000002 file:human/FGG/FGG-source-provenance.yaml |
| GO:0031012 extracellular matrix | HDA PMID:25037231 Extracellular matrix signatures of human primary metastatic ... | ACCEPT | Summary: FGG is measured in extracellular-matrix-enriched human tissue preparations. Reason: Exact FGG/P02679 supplement rows establish association. Sample, accession-group and raw-to-seed joins are retained in the source provenance. Enrichment does not establish new mechanical activity or distinguish intact precursor, gamma-A and gamma-B. |
| GO:0031012 extracellular matrix | HDA PMID:28327460 Comprehensive proteomic characterization of stem cell-derive... | UNDECIDED | Summary: FGG matrix association is established; the source-specific extension remains unverified. Reason: Exact FGG/P02679 rows in the independent PMID:25037231 and PMID:28675934 supplements establish the generic extracellular-matrix association accepted elsewhere. This raw source annotation additionally asserts part_of(CL:0002540). Its own FGG target table and specimen-specific join remain unrecovered, so the complete extended assertion remains UNDECIDED. The original extension is restored as source metadata. This is not uncertainty about generic ECM association and does not assert absence from the unverified specimen. Supporting Evidence: file:human/FGG/FGG-source-provenance.yaml |
| GO:0031012 extracellular matrix | HDA PMID:28344315 Proteomic characterization of human multiple myeloma bone ma... | UNDECIDED | Summary: FGG matrix association is established; the source-specific extension remains unverified. Reason: Exact FGG/P02679 rows in the independent PMID:25037231 and PMID:28675934 supplements establish the generic extracellular-matrix association accepted elsewhere. This raw source annotation additionally asserts part_of(UBERON:0002371). Its own FGG target table and specimen-specific join remain unrecovered, so the complete extended assertion remains UNDECIDED. The original extension is restored as source metadata. This is not uncertainty about generic ECM association and does not assert absence from the unverified specimen. Supporting Evidence: file:human/FGG/FGG-source-provenance.yaml |
| GO:0031012 extracellular matrix | HDA PMID:28675934 Characterization of the Extracellular Matrix of Normal and D... | ACCEPT | Summary: FGG is measured in extracellular-matrix-enriched human tissue preparations. Reason: Exact FGG/P02679 supplement rows establish association. Sample, accession-group and raw-to-seed joins are retained in the source provenance. Enrichment does not establish new mechanical activity or distinguish intact precursor, gamma-A and gamma-B. |
| GO:0031012 extracellular matrix | IBA GO_REF:0000033 | ACCEPT | Summary: ECM localization has positive phylogenetic and human proteomic support. Reason: PTN000441162 is ancestral to the exact human leaf. The current family table has the positive ECM IBD and no IRD records; the older API tree and exact sequence establish the persistent-node join. Human target tables corroborate association without using donor count as an evidence-quality proxy. Supporting Evidence: GO_REF:0000033 file:human/FGG/FGG-source-provenance.yaml |
| GO:0031091 platelet alpha granule | IDA PMID:6777381 Ca2+-mediated association of glycoprotein G (thrombinsensiti... | KEEP AS NON CORE | Summary: Platelet fibrinogen is a releasable alpha-granule constituent. Reason: The actual paper describes fibrinogen as alpha-granule cargo and follows its release and extracellular retention. It does not provide a gamma-specific intragranular image or splice comparison. The curated Reactome gamma-containing cargo supports the compartment, retained as a storage rather than principal structural-action location. |
| GO:0031093 platelet alpha granule lumen | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: The rule supports gamma-containing platelet granule cargo. Reason: ARBA00084788 matches the target FunFam/Homo branch. Reactome represents P02679 in platelet alpha-granule lumen before release. This is positive electronic/pathway evidence with complex scope, not a new direct gamma localization assay. Supporting Evidence: GO_REF:0000117 Reactome:R-HSA-481007 file:human/FGG/FGG-source-provenance.yaml |
| GO:0031093 platelet alpha granule lumen | TAS Reactome:R-HSA-481007 | KEEP AS NON CORE | Summary: FGG is modeled in the platelet alpha granule lumen component of this event. Reason: The actual Reactome:R-HSA-481007 component route reaches human P02679 entity 140922, modeled residues 27–453, in platelet alpha granule lumen. Exact edges are recorded in the source-provenance file. This establishes component localization, not an independent FGG catalytic/signaling claim for the event name or experimental resolution of gamma-A versus gamma-B. This is curated cargo localization, not a new gamma-specific granule image. Supporting Evidence: Reactome:R-HSA-481007 file:human/FGG/FGG-source-provenance.yaml |
| GO:0031639 plasminogen activation | IDA PMID:16846481 The fibrinogen Aalpha R16C mutation results in fibrinolytic ... | UNDECIDED | Summary: The gamma-specific cofactor contribution remains unresolved in this source. Reason: The PMID16846481 abstract studies purified fibrinogen with Aalpha R16C, reports normal plasminogen activation and delayed lysis attributed to altered clot properties. Full relevant assays were unavailable. This neither proves nor refutes gamma cofactor work. Being cleaved is insufficient, and alpha-C cofactor evidence from other papers is not transferred to gamma. Supporting Evidence: |
| GO:0031639 plasminogen activation | IEA GO_REF:0000117 | UNDECIDED | Summary: The rule match is genuine, but gamma-specific cofactor work remains unresolved. Reason: Actual target conditions and GO outputs were verified, but they expose no gamma-specific primary cofactor assay. Gamma structural participation does not automatically establish plasminogen-activation cofactor activity. Preserve uncertainty rather than declaring the inference impossible or misattributed. |
| GO:0034116 positive regulation of heterotypic cell-cell adhesion | IDA PMID:8100742 Fibrinogen mediates leukocyte adhesion to vascular endotheli... | UNDECIDED | Summary: The gamma contribution to the exact heterotypic-adhesion assay remains unresolved. Reason: PMID8100742 supports fibrinogen-mediated leukocyte/endothelial bridging in its abstract, and the rule target match is genuine. Full gamma-specific assay/control evidence was unavailable. Other gamma/ICAM-1 recognition is positive context but does not reconstruct this particular experiment; no wrong-chain conclusion is inferred. |
| GO:0034116 positive regulation of heterotypic cell-cell adhesion | IEA GO_REF:0000117 | UNDECIDED | Summary: The gamma contribution to the exact heterotypic-adhesion assay remains unresolved. Reason: PMID8100742 supports fibrinogen-mediated leukocyte/endothelial bridging in its abstract, and the rule target match is genuine. Full gamma-specific assay/control evidence was unavailable. Other gamma/ICAM-1 recognition is positive context but does not reconstruct this particular experiment; no wrong-chain conclusion is inferred. Supporting Evidence: GO_REF:0000117 file:human/FGG/FGG-source-provenance.yaml |
| GO:0042730 fibrinolysis | IDA PMID:16846481 The fibrinogen Aalpha R16C mutation results in fibrinolytic ... | UNDECIDED | Summary: The gamma-specific cofactor contribution remains unresolved in this source. Reason: The PMID16846481 abstract studies purified fibrinogen with Aalpha R16C, reports normal plasminogen activation and delayed lysis attributed to altered clot properties. Full relevant assays were unavailable. This neither proves nor refutes gamma cofactor work. Being cleaved is insufficient, and alpha-C cofactor evidence from other papers is not transferred to gamma. Supporting Evidence: |
| GO:0042730 fibrinolysis | IEA GO_REF:0000117 | UNDECIDED | Summary: The rule match is genuine, but gamma-specific cofactor work remains unresolved. Reason: Actual target conditions and GO outputs were verified, but they expose no gamma-specific primary cofactor assay. Gamma structural participation does not automatically establish plasminogen-activation cofactor activity. Preserve uncertainty rather than declaring the inference impossible or misattributed. |
| GO:0045907 positive regulation of vasoconstriction | IDA PMID:15739255 Fibrinogen and fragment D-induced vascular constriction. | KEEP AS NON CORE | Summary: Human fibrinogen and fragment D promote vascular constriction in rat preparations. Reason: Actual Methods, Results and Figures 6–8 use human fibrinogen/multichain fragment D in rat vessels with receptor/ligand-blocking and endothelin pharmacology. Gamma-region ICAM-1 recognition supports participation in the ligand complex, not an autonomous gamma vasoconstrictor or receptor-exclusive mechanism. |
| GO:0045907 positive regulation of vasoconstriction | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Human fibrinogen and fragment D promote vascular constriction in rat preparations. Reason: Actual Methods, Results and Figures 6–8 use human fibrinogen/multichain fragment D in rat vessels with receptor/ligand-blocking and endothelin pharmacology. Gamma-region ICAM-1 recognition supports participation in the ligand complex, not an autonomous gamma vasoconstrictor or receptor-exclusive mechanism. Supporting Evidence: GO_REF:0000117 file:human/FGG/FGG-source-provenance.yaml |
| GO:0045921 positive regulation of exocytosis | IDA PMID:19193866 Fibrinogen-induced endothelin-1 production from endothelial ... | KEEP AS NON CORE | Summary: Fibrinogen triggers endothelial secretory responses and ERK signaling in context. Reason: Actual PMID19193866 uses human fibrinogen on rat heart microvascular endothelial cells, measuring conditioned-medium ET-1/vWF, WPB staining and ERK phosphorylation. ICAM-1/MEK perturbations support the response with partial-blockade/feedback limits. Gamma participates in the ligand complex and has an independently mapped receptor-recognition region; it is not secretory machinery or the peptide hormone. |
| GO:0050714 positive regulation of protein secretion | IDA PMID:19193866 Fibrinogen-induced endothelin-1 production from endothelial ... | KEEP AS NON CORE | Summary: Fibrinogen triggers endothelial secretory responses and ERK signaling in context. Reason: Actual PMID19193866 uses human fibrinogen on rat heart microvascular endothelial cells, measuring conditioned-medium ET-1/vWF, WPB staining and ERK phosphorylation. ICAM-1/MEK perturbations support the response with partial-blockade/feedback limits. Gamma participates in the ligand complex and has an independently mapped receptor-recognition region; it is not secretory machinery or the peptide hormone. |
| GO:0050839 cell adhesion molecule binding | IPI PMID:7822297 Structural recognition of a novel fibrinogen gamma chain seq... | KEEP AS NON CORE | Summary: Gamma117–133 supports ICAM-1 recognition. Reason: The source abstract explicitly identifies the gamma region and receptor binding/competition. Independently read gamma-peptide adhesion experiments corroborate the same molecular claim. The informative cell-adhesion binding term is contextual rather than a duplicate core; complete source Methods remain unavailable. |
| GO:0051258 protein polymerization | IEA GO_REF:0000120 | ACCEPT | Summary: Gamma-chain interfaces contribute to fibrin polymer assembly. Reason: Actual ARBA/InterPro mappings agree with human gamma-derived peptide binding/inhibition and the recombinant gamma a-pocket structure. Gamma provides a recognition surface for exposed alpha-chain knobs; thrombin exposes the knobs and factor XIII catalyzes crosslinks. Supporting Evidence: GO_REF:0000120 file:human/FGG/FGG-source-provenance.yaml |
| GO:0051258 protein polymerization | IMP PMID:12706644 Fibrinogen Magdeburg I: a novel variant of human fibrinogen ... | ACCEPT | Summary: Gamma-chain contacts contribute directly to fibrin polymerization. Reason: Human gamma-fragment binding and inhibition experiments in PMID:6451630 and the gamma a-pocket structure in PMID:9207064 establish this gene-level polymerization role independently. Retain the existing IMP annotation with curator deference. The PMID:12706644 abstract concerns FGA Leu9Pro, delayed fibrinopeptide release and nearly normal fibrin-monomer aggregation; its full relevant assays remain unavailable. This decision neither reconstructs those experiments nor attributes the alpha-chain variant mechanism to gamma. |
| GO:0051592 response to calcium ion | IDA PMID:6777381 Ca2+-mediated association of glycoprotein G (thrombinsensiti... | KEEP AS NON CORE | Summary: Calcium affects fibrin association with activated platelet surfaces. Reason: Actual human platelet Results separate calcium-dependent extracellular retention from soluble released material and identify fibrin chains. This supports a complex-level response, not gamma as a calcium sensor or intracellular signaling enzyme. Rule provenance is documented separately. |
| GO:0051592 response to calcium ion | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Calcium affects fibrin association with activated platelet surfaces. Reason: Actual human platelet Results separate calcium-dependent extracellular retention from soluble released material and identify fibrin chains. This supports a complex-level response, not gamma as a calcium sensor or intracellular signaling enzyme. Rule provenance is documented separately. |
| GO:0065003 protein-containing complex assembly | IDA PMID:8910396 The role of betagamma and alphagamma complexes in the assemb... | ACCEPT | Summary: Gamma-containing intermediates contribute structural interfaces to complex assembly. Reason: Actual human-chain fusion experiments follow alpha-gamma/beta-gamma intermediates into full fibrinogen. This is structural participation rather than inference solely from a secretion defect or an assignment of catalytic assembly activity. Supporting Evidence: |
| GO:0070062 extracellular exosome | HDA PMID:19056867 Large-scale proteomics and phosphoproteomics of urinary exos... | KEEP AS NON CORE | Summary: FGG is detected in a urinary low-density membrane fraction. Reason: Original Main Table 1 identifies FGG with one unique peptide and one spectrum. The fraction consists chiefly of exosomes, supporting the association-level annotation. It does not resolve vesicle topology, exclude co-isolated protein or prove a gamma-A-specific peptide from the database protein name. |
| GO:0070062 extracellular exosome | HDA PMID:23533145 In-depth proteomic analyses of exosomes isolated from expres... | KEEP AS NON CORE | Summary: FGG has supported association with extracellular-exosome preparations. Reason: Independent original Main Table 1 in PMID:19056867 identifies FGG in a human urinary low-density membrane fraction consisting chiefly of exosomes, with one unique peptide and one spectrum. Retain the existing generic vesicle-association claim with curator deference. The FGG supplementary row in PMID:23533145 remains unrecovered, so its prostatic-secretion sample assignment is not independently verified. Neither source establishes intravesicular topology, exclusion of co-isolated protein or a splice-specific peptide. |
| GO:0070374 positive regulation of ERK1 and ERK2 cascade | IDA PMID:10903502 Fibrinogen interactions with ICAM-1 (CD54) regulate endothel... | KEEP AS NON CORE | Summary: Gamma-region recognition supports contextual endothelial signaling and survival. Reason: Actual PMID10903502 uses fibrinogen substrates, gamma117–133 peptide and ICAM-1 controls in TNF-alpha-exposed endothelial cells. ERK activation and reduced apoptosis support the tested downstream effects, not universal death-pathway inhibition or gamma kinase activity. Electronic mappings are not independent replicate experiments. |
| GO:0070374 positive regulation of ERK1 and ERK2 cascade | IDA PMID:19193866 Fibrinogen-induced endothelin-1 production from endothelial ... | KEEP AS NON CORE | Summary: Fibrinogen triggers endothelial secretory responses and ERK signaling in context. Reason: Actual PMID19193866 uses human fibrinogen on rat heart microvascular endothelial cells, measuring conditioned-medium ET-1/vWF, WPB staining and ERK phosphorylation. ICAM-1/MEK perturbations support the response with partial-blockade/feedback limits. Gamma participates in the ligand complex and has an independently mapped receptor-recognition region; it is not secretory machinery or the peptide hormone. |
| GO:0070527 platelet aggregation | IBA GO_REF:0000033 | ACCEPT | Summary: Platelet aggregation has positive ancestry and human gamma-chain support. Reason: The positive PTN002709696 IBD lies on the exact target path; self-WITH is expected target grounding. The ARBA match and human gamma preparation support bridging. The isolated chains form multimers; this is not a soluble-monomer or natural-splice comparison. |
| GO:0070527 platelet aggregation | IDA PMID:6281794 gamma and alpha chains of human fibrinogen possess sites rea... | ACCEPT | Summary: Human gamma-chain preparations support platelet aggregation. Reason: Actual Methods, Results, Tables 1–2 and Figure 1 distinguish ADP-stimulated platelets, gamma multimers and intact fibrinogen, with antibody inhibition. Gamma is strongly active in this preparation, alpha weaker and beta inactive. Multivalency/preparation limits preclude a monomer-agonist claim or equal activity of the two natural splice products. Supporting Evidence: |
| GO:0070527 platelet aggregation | IEA GO_REF:0000117 | ACCEPT | Summary: Platelet aggregation has positive ancestry and human gamma-chain support. Reason: The positive PTN002709696 IBD lies on the exact target path; self-WITH is expected target grounding. The ARBA match and human gamma preparation support bridging. The isolated chains form multimers; this is not a soluble-monomer or natural-splice comparison. |
| GO:0072378 blood coagulation, fibrin clot formation | IBA GO_REF:0000033 | ACCEPT | Summary: Gamma structural contacts contribute to fibrin clot formation. Reason: The positive target-path IBD and actual ARBA match agree with human gamma-fragment binding and a-pocket structure. This is structural participation, not a process assignment solely because gamma is a substrate for another enzyme. Supporting Evidence: GO_REF:0000033 file:human/FGG/FGG-source-provenance.yaml |
| GO:0072378 blood coagulation, fibrin clot formation | IDA PMID:16846481 The fibrinogen Aalpha R16C mutation results in fibrinolytic ... | ACCEPT | Summary: Gamma contributes to the fibrin structure formed by the tested complex. Reason: The source abstract reports clot formation with purified alpha-variant fibrinogen. Independently read gamma-binding/structure experiments establish the same generic clot-formation role. Source-specific activation/lysis claims remain unresolved; the alpha variant is not relabeled a gamma mutation. |
| GO:0072378 blood coagulation, fibrin clot formation | IEA GO_REF:0000117 | ACCEPT | Summary: Gamma structural contacts contribute to fibrin clot formation. Reason: The positive target-path IBD and actual ARBA match agree with human gamma-fragment binding and a-pocket structure. This is structural participation, not a process assignment solely because gamma is a substrate for another enzyme. Supporting Evidence: GO_REF:0000117 file:human/FGG/FGG-source-provenance.yaml |
| GO:0072378 blood coagulation, fibrin clot formation | IMP PMID:11001902 Afibrinogenemia: first identification of a splicing mutation... | ACCEPT | Summary: Gamma-chain structural contacts contribute to fibrin clot formation. Reason: Independent human gamma-fragment and a-pocket evidence establishes direct structural participation in fibrin formation. The original PMID:11001902 clinical/minigene Results support aberrant RNA and a predicted severe truncation with loss of circulating fibrinogen; the predicted protein was not directly demonstrated. Retain that disease-context evidence without treating necessity alone as the structural mechanism. The pathological/engineered truncation is not a natural splice product. |
| GO:0072562 blood microparticle | HDA PMID:22516433 Proteomic analysis of microvesicles from plasma of healthy d... | UNDECIDED | Summary: The source-specific vesicle-associated target join remains unresolved. Reason: The survey/sample context is accessible but its exact FGG peptide/protein table was not recovered. Positive detection in another publication does not verify this specimen-specific assertion; absence or misattribution is not inferred. Supporting Evidence: |
| GO:0090277 positive regulation of peptide hormone secretion | IDA PMID:19193866 Fibrinogen-induced endothelin-1 production from endothelial ... | KEEP AS NON CORE | Summary: Fibrinogen triggers endothelial secretory responses and ERK signaling in context. Reason: Actual PMID19193866 uses human fibrinogen on rat heart microvascular endothelial cells, measuring conditioned-medium ET-1/vWF, WPB staining and ERK phosphorylation. ICAM-1/MEK perturbations support the response with partial-blockade/feedback limits. Gamma participates in the ligand complex and has an independently mapped receptor-recognition region; it is not secretory machinery or the peptide hormone. |
| GO:0090277 positive regulation of peptide hormone secretion | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Fibrinogen triggers endothelial secretory responses and ERK signaling in context. Reason: Actual PMID19193866 uses human fibrinogen on rat heart microvascular endothelial cells, measuring conditioned-medium ET-1/vWF, WPB staining and ERK phosphorylation. ICAM-1/MEK perturbations support the response with partial-blockade/feedback limits. Gamma participates in the ligand complex and has an independently mapped receptor-recognition region; it is not secretory machinery or the peptide hormone. Supporting Evidence: GO_REF:0000117 file:human/FGG/FGG-source-provenance.yaml |
| GO:0140149 non-collagenous component of interstitial matrix | TAS PMID:36399478 MatrisomeDB 2.0: 2023 updates to the ECM-protein knowledge d... | UNDECIDED | Summary: The exact MatrisomeDB interstitial-matrix assignment remains unresolved. Reason: The database paper describes ECM integration, but the FGG-specific record for this finer subcompartment was not recovered. Human proteomics supports broad ECM association, not every tissue subcompartment. Preserve this TAS context pending its target record. Supporting Evidence: |
| GO:1900026 positive regulation of substrate adhesion-dependent cell spreading | NAS PMID:24041635 The role of fibrin matrices and tissue factor in early-term ... | UNDECIDED | Summary: The gamma role in trophoblast spreading remains unresolved. Reason: The abstract reports primary human trophoblast responses to fibrin matrices and factor-XIII conditions. Full relevant results were unavailable and the abstract does not isolate gamma participation in the spreading response. General structure or other-cell adhesion assays do not settle this NAS context. Supporting Evidence: |
| GO:1902042 negative regulation of extrinsic apoptotic signaling pathway via death domain receptors | IDA PMID:10903502 Fibrinogen interactions with ICAM-1 (CD54) regulate endothel... | KEEP AS NON CORE | Summary: Gamma-region recognition supports contextual endothelial signaling and survival. Reason: Actual PMID10903502 uses fibrinogen substrates, gamma117–133 peptide and ICAM-1 controls in TNF-alpha-exposed endothelial cells. ERK activation and reduced apoptosis support the tested downstream effects, not universal death-pathway inhibition or gamma kinase activity. Electronic mappings are not independent replicate experiments. |
| GO:1902042 negative regulation of extrinsic apoptotic signaling pathway via death domain receptors | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Gamma-region recognition supports contextual endothelial signaling and survival. Reason: Actual PMID10903502 uses fibrinogen substrates, gamma117–133 peptide and ICAM-1 controls in TNF-alpha-exposed endothelial cells. ERK activation and reduced apoptosis support the tested downstream effects, not universal death-pathway inhibition or gamma kinase activity. Electronic mappings are not independent replicate experiments. |
| GO:2000352 negative regulation of endothelial cell apoptotic process | IDA PMID:10903502 Fibrinogen interactions with ICAM-1 (CD54) regulate endothel... | KEEP AS NON CORE | Summary: Gamma-region recognition supports contextual endothelial signaling and survival. Reason: Actual PMID10903502 uses fibrinogen substrates, gamma117–133 peptide and ICAM-1 controls in TNF-alpha-exposed endothelial cells. ERK activation and reduced apoptosis support the tested downstream effects, not universal death-pathway inhibition or gamma kinase activity. Electronic mappings are not independent replicate experiments. |
| GO:2000352 negative regulation of endothelial cell apoptotic process | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Gamma-region recognition supports contextual endothelial signaling and survival. Reason: Actual PMID10903502 uses fibrinogen substrates, gamma117–133 peptide and ICAM-1 controls in TNF-alpha-exposed endothelial cells. ERK activation and reduced apoptosis support the tested downstream effects, not universal death-pathway inhibition or gamma kinase activity. Electronic mappings are not independent replicate experiments. |
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