GTF2F2 (RAP30, TFIIF-beta) is the smaller (~30 kDa) subunit of general transcription factor TFIIF, a heterodimer it forms with GTF2F1 (RAP74, TFIIF-alpha). TFIIF is one of the general transcription factors required for RNA polymerase II (Pol II)-dependent transcription. RAP30 binds Pol II and, together with RAP74, escorts the polymerase into the growing preinitiation complex assembled on promoter DNA by TBP/TFIID, TFIIA and TFIIB, and it suppresses nonspecific (non-promoter) binding of Pol II to DNA. Within the preinitiation complex, the C-terminal winged-helix/HTH domain of RAP30 (structurally homologous to linker histone H5) contacts promoter DNA downstream of the TATA box and helps position the polymerase and stabilize promoter DNA, contributing to start-site selection and promoter opening together with TFIIE and TFIIH. The N-terminal region mediates dimerization with RAP74 and contacts with TFIIB and Pol II. Beyond initiation, TFIIF remains associated during early transcription, where it promotes synthesis of the first phosphodiester bonds, suppresses transient pausing and backtracking by stabilizing the post-translocated elongation complex, and stimulates the elongation rate of Pol II. GTF2F2 acts in the nucleus (nucleoplasm), is constitutively expressed across tissues, and is a core component of the basal Pol II transcription machinery used at most protein-coding and snRNA promoters.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0006367 transcription initiation at RNA polymerase II promoter | IBA GO_REF:0000033 | ACCEPT | Summary: TFIIF/RAP30 is a general transcription factor required for accurate Pol II transcription initiation at promoters; it binds Pol II and helps recruit it to the preinitiation complex in collaboration with TFIIB. This is the core, phylogenetically conserved function of the protein. Reason: This IBA annotation captures the central, well-established biological process of GTF2F2 and is supported by both direct biochemistry and UniProt's curated function statement. It is consistent across orthologs in the PANTHER family (PTN000047872). Supporting Evidence: PMID:7929273 Results of template competition experiments indicate that both RAP30 and RAP74 contribute to the formation of stable preinitiation intermediates containing RNA polymerase II. file:human/GTF2F2/GTF2F2-deep-research-falcon.md TFIIF recruits/guides Pol II into the TBP-TFIIA-TFIIB promoter complex, suppresses nonspecific DNA binding, stabilizes the PIC, promotes promoter opening with TFIIE/TFIIH |
| GO:0005674 transcription factor TFIIF complex | IBA GO_REF:0000033 | ACCEPT | Summary: GTF2F2/RAP30 is one of the two subunits of the heterodimeric general transcription factor TFIIF complex (with GTF2F1/RAP74). This is the defining complex membership of the protein. Reason: Direct structural and biochemical evidence establishes that RAP30 forms a heterodimer with RAP74 to constitute TFIIF; the IBA assignment is consistent with the curated ComplexPortal complex (CPX-79) and is at the correct level of specificity. Supporting Evidence: PMID:11183778 General transcription factor IIF (TFIIF) is required for transcription by RNA polymerase II; it consists minimally of a heterodimer of RNA polymerase-associated proteins RAP30 and RAP74. |
| GO:0003677 DNA binding | IEA GO_REF:0000043 | ACCEPT | Summary: RAP30 contains a C-terminal winged-helix/HTH DNA-binding domain (structurally homologous to linker histone H5) that contacts promoter DNA within the preinitiation complex, with specific DNA-contacting residues resolved structurally. DNA binding is a genuine molecular activity of the protein, though it is sequence-nonspecific and operates in the context of the assembled complex. Reason: The keyword-derived DNA binding annotation is corroborated by NMR structure of the RAP30 DNA-binding domain and by cryo-EM that identifies DNA-contacting residues (e.g., positions 227 and 229), making the broad GO:0003677 term appropriate. Supporting Evidence: PMID:11183778 the multiple domains of RAP30 and RAP74 bind PolII, TFIIB, TAF250 and DNA in interactions that are essential for transcription initiation and elongation. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | ACCEPT | Summary: GTF2F2 acts in the nucleus as part of the Pol II transcription machinery. Nuclear localization is documented experimentally. Reason: Nuclear localization is consistent with the protein's function in Pol II transcription and is independently supported by experimental IDA annotations; the broad nucleus term is appropriate even though the more specific nucleoplasm term also applies. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Subcellular localization Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005674 transcription factor TFIIF complex | IEA GO_REF:0000002 | ACCEPT | Summary: InterPro-based assignment of TFIIF complex membership, redundant with the IBA and IPI annotations to the same term. Reason: Correct complex membership for the TFIIF beta subunit (InterPro IPR003196 = TFIIF_beta); this duplicates the experimentally and phylogenetically supported GO:0005674 annotations and is at the right level of specificity. Supporting Evidence: PMID:11183778 it consists minimally of a heterodimer of RNA polymerase-associated proteins RAP30 and RAP74. |
| GO:0006366 transcription by RNA polymerase II | IEA GO_REF:0000002 | ACCEPT | Summary: InterPro-based assignment to the general Pol II transcription process. GTF2F2 is a core general transcription factor for Pol II. Reason: This is a correct but broad parent process; it is consistent with the more specific initiation and elongation annotations and is supported by experimental IMP and TAS annotations to the same term. Supporting Evidence: PMID:7929273 TFIIF has been shown to bind RNA polymerase II and control the activity of the enzyme in both the initiation and elongation stages of transcription. |
| GO:0006367 transcription initiation at RNA polymerase II promoter | IEA GO_REF:0000120 | ACCEPT | Summary: Combined-IEA assignment to Pol II transcription initiation, the core function of TFIIF, redundant with the IBA/ISS/IDA annotations to the same term. Reason: Correct and well-supported core process; duplicates experimentally supported annotations to GO:0006367. Supporting Evidence: PMID:7929273 both RAP30 and RAP74 function in synthesis of the first few phosphodiester bonds of nascent transcripts and in formation of Sarkosyl-resistant pre-initiation intermediates. |
| GO:0006368 transcription elongation by RNA polymerase II | IEA GO_REF:0000117 | ACCEPT | Summary: ARBA machine-learning assignment to Pol II elongation. TFIIF stimulates the rate of Pol II elongation and suppresses transient pausing. Reason: Correct process supported by direct biochemistry; redundant with IDA (PMID:15351637) and TAS (PMID:7929273) annotations to the same term. Supporting Evidence: PMID:7929273 kinetic experiments indicate that both RAP30 and RAP74 function in TFIIF-mediated stimulation of the rate of RNA chain elongation by RNA polymerase II. |
| GO:0016591 RNA polymerase II, holoenzyme | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: ARBA assignment placing GTF2F2 as part of the Pol II holoenzyme. TFIIF associates with Pol II as part of the preinitiation complex. Reason: The protein does associate with Pol II within the preinitiation complex, so the annotation is not wrong, but the precise and preferred complex annotation for GTF2F2 is the TFIIF complex (GO:0005674). The holoenzyme term is a broader/looser localization that is acceptable but not the core complex membership. Supporting Evidence: PMID:7929273 TFIIF has been shown to bind RNA polymerase II and control the activity of the enzyme in both the initiation and elongation stages of transcription. |
| GO:0090575 RNA polymerase II transcription regulator complex | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: ARBA assignment to the broad Pol II transcription regulator complex category. Reason: GTF2F2 is part of a Pol II-associated transcription complex, so this broad parent grouping is defensible, but the specific and preferred complex annotation is the TFIIF complex (GO:0005674). Retained as non-core because it is less informative than the specific complex term. Supporting Evidence: PMID:11183778 General transcription factor IIF (TFIIF) is required for transcription by RNA polymerase II |
| GO:0005515 protein binding | IPI PMID:11183778 Novel dimerization fold of RAP30/RAP74 in human TFIIF at 1.7... | MODIFY | Summary: This IPI is the RAP30-RAP74 (GTF2F1) dimerization interaction determined by X-ray crystallography of the TFIIF interaction domains. This is the defining heterodimer interaction of the protein. Reason: Bare protein binding is uninformative. The interactor is the partner subunit GTF2F1/RAP74 and the relationship is TFIIF complex formation; GO:0044877 (protein-containing complex binding) is more informative and captures that RAP30 binds within the TFIIF complex. The complex membership itself is already captured by the GO:0005674 annotation. Proposed replacements: protein-containing complex binding Supporting Evidence: PMID:11183778 The X-ray structure of the RAP30/RAP74 interaction domains at 1.7 A resolution reveals a novel "triple barrel" dimerization fold |
| GO:0005515 protein binding | IPI PMID:12737519 Interaction with general transcription factor IIF (TFIIF) is... | ACCEPT | Summary: IPI capturing interaction of RAP30/TFIIF with URI1 (RMP), an RPB5- mediating corepressor that binds both RAP30 and RAP74 to suppress activated transcription. Reason: This is a specific, experimentally validated protein-protein interaction (pull-down, Far-Western, co-IP) with a defined biological consequence. Although the term is the generic protein binding, the interaction is real and documented; retaining as a binding annotation is acceptable. The functional context (transcriptional repression via TFIIF) is informative. Supporting Evidence: PMID:12737519 we demonstrated that RMP could bind with bacterially expressed recombinant RAP30 and RAP74 of TFIIF subunits. |
| GO:0005515 protein binding | IPI PMID:20195357 A comprehensive resource of interacting protein regions for ... | KEEP AS NON CORE | Summary: High-throughput interaction screen (IVV/mRNA-display based mapping of transcription factor networks) reporting interactions of GTF2F2, including with URI1 and RPL27A. Reason: This is a generic protein binding annotation from a large-scale interactome resource. It does not identify a specific informative molecular function and the individual interactions are not independently validated here. Retained as non-core supporting evidence rather than removed, since TFIIF is genuinely a hub in transcription factor networks. Supporting Evidence: PMID:20195357 A comprehensive resource of interacting protein regions for refining human transcription factor networks. |
| GO:0005515 protein binding | IPI PMID:24981860 Human-chromatin-related protein interactions identify a deme... | KEEP AS NON CORE | Summary: Affinity-purification/MS interactome study of chromatin-related proteins reporting an interaction involving GTF2F2 (with GTF2F1/RAP74). Reason: Generic protein binding from a high-throughput chromatin interactome screen. The partner (RAP74) is the expected TFIIF dimer partner, so the hit is biologically plausible, but the term itself is uninformative. Kept as non-core supporting evidence. Supporting Evidence: PMID:24981860 Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation. |
| GO:0005515 protein binding | IPI PMID:31467278 Maximizing binary interactome mapping with a minimal number ... | KEEP AS NON CORE | Summary: Binary interactome mapping methodology study reporting a GTF2F2 interaction with GTF2F1/RAP74. Reason: Generic protein binding derived from a high-throughput binary interactome assay; the recovered partner is the canonical TFIIF dimer partner. Not informative as a molecular function term, retained as non-core corroboration of the RAP30-RAP74 interaction. Supporting Evidence: PMID:31467278 Maximizing binary interactome mapping with a minimal number of assays. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | KEEP AS NON CORE | Summary: HuRI reference binary interactome reporting GTF2F2 interactions (e.g., FHL2, ZMYND19, MAPRE3). Reason: Generic protein binding from a systematic binary interactome map. These hits are not individually validated and do not specify an informative molecular function. Some (e.g., MAPRE3/EB3) may underlie the spurious microtubule-cytoskeleton localization. Retained as non-core. Supporting Evidence: PMID:32296183 A reference map of the human binary protein interactome. |
| GO:0005515 protein binding | IPI PMID:37398436 AI-guided pipeline for protein-protein interaction drug disc... | KEEP AS NON CORE | Summary: AI-guided PPI drug-discovery pipeline (binary interactome platform) reporting an interaction involving GTF2F2 and GTF2F1/RAP74. Reason: Generic protein binding from a high-throughput interaction platform; the partner is the expected TFIIF dimer subunit. Uninformative as a molecular function term, retained as non-core supporting data. Supporting Evidence: PMID:37398436 AI-guided pipeline for protein-protein interaction drug discovery identifies a SARS-CoV-2 inhibitor. |
| GO:0005515 protein binding | IPI PMID:7854423 Interaction with RAP74 subunit of TFIIF is required for tran... | KEEP AS NON CORE | Summary: This annotation derives from a study showing serum response factor (SRF) binds the RAP74 subunit of TFIIF; the IntAct WITH field records the interaction against GTF2F1/RAP74 (P35269), i.e. RAP30's dimer partner. Reason: The primary finding (SRF activation domain binding RAP74) concerns GTF2F1 rather than a direct RAP30 contact, and the annotation here is the generic protein binding term with RAP74 as the recorded partner. The RAP30-RAP74 dimerization is already better captured elsewhere; retained as non-core because it does not define an informative RAP30 molecular function. Supporting Evidence: PMID:7854423 we find that SRF binds the RAP74 subunit of TFIIF and that SRF's transcriptional activation domain is the region involved in this binding. |
| GO:0005674 transcription factor TFIIF complex | IPI PMID:11183778 Novel dimerization fold of RAP30/RAP74 in human TFIIF at 1.7... | ACCEPT | Summary: ComplexPortal-curated TFIIF complex membership (CPX-79) for RAP30, based on the crystal structure of the RAP30/RAP74 heterodimer. Reason: Correct, experimentally grounded complex membership at the right level of specificity. This is the preferred complex annotation for GTF2F2. Supporting Evidence: PMID:11183778 it consists minimally of a heterodimer of RNA polymerase-associated proteins RAP30 and RAP74. |
| GO:0006367 transcription initiation at RNA polymerase II promoter | IDA PMID:15351637 Transcription factors IIF and IIS and nucleoside triphosphat... | ACCEPT | Summary: ComplexPortal IDA annotation of TFIIF to Pol II transcription initiation, based on transient-state kinetic dissection of the human Pol II mechanism in the presence of TFIIF. Reason: Core process supported by direct in vitro biochemistry showing TFIIF acting on the Pol II initiation/early synthesis mechanism. Redundant with the IBA/IEA/ISS annotations to the same term but appropriately experimental. Supporting Evidence: PMID:15351637 We report adequate two-bond kinetic simulations for the reaction in the presence of TFIIF alone and in the presence of TFIIF+TFIIS, providing detailed insight into the RNAP II mechanism |
| GO:0006368 transcription elongation by RNA polymerase II | IDA PMID:15351637 Transcription factors IIF and IIS and nucleoside triphosphat... | ACCEPT | Summary: ComplexPortal IDA annotation of TFIIF to Pol II elongation. The study shows TFIIF stabilizes the post-translocated elongation complex, supporting forward synthesis and suppressing pausing. Reason: Directly supported by kinetic data showing TFIIF supports elongation and suppresses pausing by stabilizing the post-translocated elongation complex; this is a genuine core function of TFIIF. Supporting Evidence: PMID:15351637 TFIIF supports elongation and suppresses pausing by stabilizing the post-translocated elongation complex. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IDA PMID:15351637 Transcription factors IIF and IIS and nucleoside triphosphat... | ACCEPT | Summary: ComplexPortal IDA annotation reflecting that TFIIF positively stimulates Pol II activity (forward synthesis and elongation rate). Reason: TFIIF positively contributes to Pol II transcription output by promoting initiation and stimulating the elongation rate; the positive regulation term is supported by the functional data and by TFIIF's role in suppressing pausing/abortive cycles. Supporting Evidence: PMID:7929273 both RAP30 and RAP74 function in TFIIF-mediated stimulation of the rate of RNA chain elongation by RNA polymerase II. |
| GO:0005654 nucleoplasm | IDA GO_REF:0000052 | ACCEPT | Summary: HPA immunofluorescence localizes GTF2F2 to the nucleoplasm, the compartment where Pol II transcription occurs. Reason: Nucleoplasmic localization is the precise and expected compartment for a Pol II general transcription factor and is supported by curated immunofluorescence; consistent with the broader nucleus annotations. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Subcellular localization Nuclear, chromatin-associated; localized at core promoters within the PIC |
| GO:0015630 microtubule cytoskeleton | IDA GO_REF:0000052 | REMOVE | Summary: HPA immunofluorescence reported a microtubule-cytoskeleton signal. This is inconsistent with the established biology of GTF2F2 as a nuclear Pol II general transcription factor. Reason: There is no biochemical, structural, or mechanistic support for a cytoskeletal role of RAP30/TFIIF, which functions in the nucleus within the Pol II preinitiation and elongation complexes. This single HPA immunofluorescence localization most likely reflects antibody cross-reactivity or staining artifact (possibly related to interactome hits such as MAPRE3/EB3) and should not be retained as a genuine localization. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0006366 transcription by RNA polymerase II | IMP PMID:8662660 RNA polymerase II-associated protein (RAP) 74 binds transcri... | ACCEPT | Summary: This UniProt IMP reflects mutational dissection of RAP30 functional domains, in which N- and C-terminal RAP30 sequences were shown to be required for accurate Pol II transcription, RAP74 binding and TFIIB binding. Reason: Domain-mutant transcription assays directly demonstrate that RAP30 sequences are required for accurate Pol II transcription, supporting the broad process term. Redundant with the IEA/TAS annotations to GO:0006366 but experimentally grounded. Supporting Evidence: PMID:8662660 Transcription assays indicate the importance of both N- and C-terminal sequences for RAP30 function. |
| GO:0006367 transcription initiation at RNA polymerase II promoter | ISS GO_REF:0000024 | ACCEPT | Summary: ISS transfer from an ortholog (UniProtKB:Q01750) of the core TFIIF initiation function. Reason: Sequence-similarity transfer of the well-established core process is appropriate and concordant with the IBA and experimental annotations to GO:0006367. Supporting Evidence: PMID:7929273 both RAP30 and RAP74 contribute to the formation of stable preinitiation intermediates containing RNA polymerase II. |
| GO:0016251 RNA polymerase II general transcription initiation factor activity | ISS GO_REF:0000024 | ACCEPT | Summary: ISS assignment of the molecular function general transcription initiation factor activity for Pol II, transferred from an ortholog. This is the most informative molecular-function term for GTF2F2/TFIIF. Reason: This is the appropriate, specific molecular function for the protein and is well supported by direct biochemistry showing TFIIF is required for accurate Pol II initiation. It is preferred over the generic protein binding annotations as the representative MF. Supporting Evidence: PMID:11183778 General transcription factor IIF (TFIIF) is required for transcription by RNA polymerase II |
| GO:0005515 protein binding | IPI PMID:8662660 RNA polymerase II-associated protein (RAP) 74 binds transcri... | MODIFY | Summary: IPI capturing the direct RAP30-TFIIB (GTF2B) interaction; TFIIB binds an overlapping N-terminal region of RAP30, and this contact is part of preinitiation complex assembly. Reason: Bare protein binding is uninformative. The interactor is the general transcription factor TFIIB (GTF2B), so the more specific MF term GO:0001091 (RNA polymerase II general transcription initiation factor binding) better describes this contact within the basal machinery. Proposed replacements: RNA polymerase II general transcription initiation factor binding Supporting Evidence: PMID:8662660 TFIIB binds to an overlapping region of RAP30, localized to amino acids 1-176 (amino acids 27-152 comprise a minimal binding region). |
| GO:0005515 protein binding | IPI PMID:8504927 Multiple functional domains of human transcription factor II... | MODIFY | Summary: IPI capturing the interaction between TFIIB (GTF2B) and the small subunit of TFIIF (RAP30); the N-terminus of TFIIB (including its zinc finger) contacts RAP30. Reason: The partner is the general transcription factor TFIIB, so the specific term GO:0001091 (RNA polymerase II general transcription initiation factor binding) is more informative than bare protein binding and reflects the role of this contact in PIC formation. Proposed replacements: RNA polymerase II general transcription initiation factor binding Supporting Evidence: PMID:8504927 The interaction with the small subunit of TFIIF was mapped to the amino terminus of TFIIB, which includes a zinc finger. |
| GO:0005634 nucleus | IDA PMID:9841876 Oestrogen receptor facilitates the formation of preinitiatio... | ACCEPT | Summary: CAFA-curated IDA nuclear localization, from a study of TFIIB/TFIIF recruitment during estrogen-receptor-facilitated preinitiation complex assembly. Reason: Nuclear localization is correct and expected for a Pol II general transcription factor; concordant with the IEA, HDA, and IDA nucleoplasm annotations. Supporting Evidence: PMID:9841876 recombinant human ER increased the stable association of subsequent components of the transcription machinery (TFIIE and TFIIF) |
| GO:0005634 nucleus | HDA PMID:16791210 Dynamic proteomics in individual human cells uncovers widesp... | ACCEPT | Summary: High-throughput dynamic-proteomics study classifying GTF2F2 as a nuclear protein. Reason: Consistent with the established nuclear localization of the protein; supports the nucleus/nucleoplasm annotations even though derived from a high-throughput dataset. Supporting Evidence: PMID:16791210 Dynamic proteomics in individual human cells uncovers widespread cell-cycle dependence of nuclear proteins. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-109638 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-109639 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-111264 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112379 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112381 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112383 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112385 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112392 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112395 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-112396 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113402 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113407 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113409 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113411 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113412 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113413 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113414 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113429 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-113430 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-170704 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-170706 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6797606 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6797616 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6803523 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6803527 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6803836 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6803838 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6810233 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6810234 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6810235 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6810238 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6814549 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6814555 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6814559 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-6814885 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-72095 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-72103 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-73946 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75079 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75080 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75081 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75082 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75083 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75095 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75850 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75856 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75861 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75862 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75864 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75866 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75869 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75873 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75891 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-75949 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-76576 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77068 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77069 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77071 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77073 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77077 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77078 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77081 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77083 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77085 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77090 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-77095 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9012315 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9012319 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9613494 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9613497 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9770119 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9770129 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9770131 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9770132 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9770141 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9770142 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9770145 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9770236 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9770847 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9794542 | ACCEPT | Summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome reactions covering Pol II preinitiation, initiation, promoter escape, elongation, capping, splicing and related steps. The localization is correct; the many reaction-specific references reflect Reactome pathway granularity rather than distinct localizations. Reason: Nucleoplasm is the correct compartment for a Pol II general transcription factor and is independently supported by HPA immunofluorescence (IDA) and experimental nucleus annotations. These TAS entries are redundant with each other but not incorrect; some downstream reactions (e.g. splicing, capping) reflect pathway membership of the broader machinery rather than a direct GTF2F2 role, but the nucleoplasm localization itself is accurate. Supporting Evidence: file:human/GTF2F2/GTF2F2-deep-research-falcon.md Nuclear, chromatin-associated; localized at core promoters within the PIC and detectable in early elongation complexes |
| GO:0006366 transcription by RNA polymerase II | TAS PMID:7929273 Roles for both the RAP30 and RAP74 subunits of transcription... | ACCEPT | Summary: TAS annotation to Pol II transcription, from a study establishing that both RAP30 and RAP74 contribute to Pol II transcription initiation and elongation. Reason: Author-stated, experimentally grounded assignment of the core process; redundant with the IMP and IEA annotations to GO:0006366. Supporting Evidence: PMID:7929273 Roles for both the RAP30 and RAP74 subunits of transcription factor IIF in transcription initiation and elongation by RNA polymerase II. |
| GO:0006368 transcription elongation by RNA polymerase II | TAS PMID:7929273 Roles for both the RAP30 and RAP74 subunits of transcription... | ACCEPT | Summary: TAS annotation to Pol II elongation; the cited study shows both RAP30 and RAP74 function in TFIIF-mediated stimulation of the Pol II elongation rate. Reason: Author-stated, experimentally supported assignment of a genuine core function (elongation stimulation); concordant with IDA (PMID:15351637) and IEA annotations to GO:0006368. Supporting Evidence: PMID:7929273 Roles for both the RAP30 and RAP74 subunits of transcription factor IIF in transcription initiation and elongation by RNA polymerase II. |
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Download this section (compressed HTML)Q: Does the RAP30 winged-helix DNA-binding domain make sequence-nonspecific versus sequence-influenced contacts that contribute to transcription start-site selection in human promoters?
Suggested experts: Cramer P, Nogales E
Q: Are the reported high-throughput interactome partners of GTF2F2 outside the core transcription machinery (e.g. FHL2, ZMYND19, MAPRE3) functionally meaningful, or do they represent assay artifacts?
Suggested experts: Vidal M
Experiment: Reconstitute human Pol II transcription in vitro with wild-type versus winged-helix-domain mutant RAP30 and map transcription start sites and promoter DNA contacts (e.g. by primer extension and crosslinking/cryo-EM).
Hypothesis: The RAP30 C-terminal winged-helix domain is required for correct Pol II start-site selection and promoter-proximal positioning, distinct from its role in RAP74 dimerization.
Type: in vitro reconstituted transcription and structural mapping
Experiment: Acutely deplete GTF2F2 (e.g. degron) in human cells and measure nascent transcription genome-wide (e.g. PRO-seq/TT-seq) to quantify effects on initiation, pause-release, and elongation rate.
Hypothesis: GTF2F2 is broadly required for Pol II transcription across most active promoters, with measurable effects on elongation rate and promoter-proximal pausing.
Type: acute depletion with nascent transcription profiling
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