GTF2F2

id: P13984
gene_symbol: GTF2F2
product_type: PROTEIN
status: COMPLETE
taxon:
  id: NCBITaxon:9606
  label: Homo sapiens
description: GTF2F2 (RAP30, TFIIF-beta) is the smaller (~30 kDa) subunit of general
  transcription factor TFIIF, a heterodimer it forms with GTF2F1 (RAP74, TFIIF-alpha).
  TFIIF is one of the general transcription factors required for RNA polymerase II
  (Pol II)-dependent transcription. RAP30 binds Pol II and, together with RAP74,
  escorts the polymerase into the growing preinitiation complex assembled on
  promoter DNA by TBP/TFIID, TFIIA and TFIIB, and it suppresses nonspecific
  (non-promoter) binding of Pol II to DNA. Within the preinitiation complex, the
  C-terminal winged-helix/HTH domain of RAP30 (structurally homologous to linker
  histone H5) contacts promoter DNA downstream of the TATA box and helps position
  the polymerase and stabilize promoter DNA, contributing to start-site selection
  and promoter opening together with TFIIE and TFIIH. The N-terminal region
  mediates dimerization with RAP74 and contacts with TFIIB and Pol II. Beyond
  initiation, TFIIF remains associated during early transcription, where it
  promotes synthesis of the first phosphodiester bonds, suppresses transient
  pausing and backtracking by stabilizing the post-translocated elongation
  complex, and stimulates the elongation rate of Pol II. GTF2F2 acts in the
  nucleus (nucleoplasm), is constitutively expressed across tissues, and is a core
  component of the basal Pol II transcription machinery used at most protein-coding
  and snRNA promoters.
existing_annotations:
  - term:
      id: GO:0006367
      label: transcription initiation at RNA polymerase II promoter
    evidence_type: IBA
    original_reference_id: GO_REF:0000033
    review:
      summary: TFIIF/RAP30 is a general transcription factor required for accurate
        Pol II transcription initiation at promoters; it binds Pol II and helps
        recruit it to the preinitiation complex in collaboration with TFIIB. This
        is the core, phylogenetically conserved function of the protein.
      action: ACCEPT
      reason: This IBA annotation captures the central, well-established biological
        process of GTF2F2 and is supported by both direct biochemistry and
        UniProt's curated function statement. It is consistent across orthologs in
        the PANTHER family (PTN000047872).
      supported_by:
        - reference_id: PMID:7929273
          supporting_text: Results of template competition experiments indicate that
            both RAP30 and RAP74 contribute to the formation of stable
            preinitiation intermediates containing RNA polymerase II.
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: TFIIF recruits/guides Pol II into the TBP-TFIIA-TFIIB
            promoter complex, suppresses nonspecific DNA binding, stabilizes the
            PIC, promotes promoter opening with TFIIE/TFIIH
  - term:
      id: GO:0005674
      label: transcription factor TFIIF complex
    evidence_type: IBA
    original_reference_id: GO_REF:0000033
    review:
      summary: GTF2F2/RAP30 is one of the two subunits of the heterodimeric general
        transcription factor TFIIF complex (with GTF2F1/RAP74). This is the defining
        complex membership of the protein.
      action: ACCEPT
      reason: Direct structural and biochemical evidence establishes that RAP30
        forms a heterodimer with RAP74 to constitute TFIIF; the IBA assignment is
        consistent with the curated ComplexPortal complex (CPX-79) and is at the
        correct level of specificity.
      supported_by:
        - reference_id: PMID:11183778
          supporting_text: General transcription factor IIF (TFIIF) is required for
            transcription by RNA polymerase II; it consists minimally of a
            heterodimer of RNA polymerase-associated proteins RAP30 and RAP74.
  - term:
      id: GO:0003677
      label: DNA binding
    evidence_type: IEA
    original_reference_id: GO_REF:0000043
    review:
      summary: RAP30 contains a C-terminal winged-helix/HTH DNA-binding domain
        (structurally homologous to linker histone H5) that contacts promoter DNA
        within the preinitiation complex, with specific DNA-contacting residues
        resolved structurally. DNA binding is a genuine molecular activity of the
        protein, though it is sequence-nonspecific and operates in the context of
        the assembled complex.
      action: ACCEPT
      reason: The keyword-derived DNA binding annotation is corroborated by NMR
        structure of the RAP30 DNA-binding domain and by cryo-EM that identifies
        DNA-contacting residues (e.g., positions 227 and 229), making the broad
        GO:0003677 term appropriate.
      supported_by:
        - reference_id: PMID:11183778
          supporting_text: the multiple domains of RAP30 and RAP74 bind PolII,
            TFIIB, TAF250 and DNA in interactions that are essential for
            transcription initiation and elongation.
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IEA
    original_reference_id: GO_REF:0000044
    review:
      summary: GTF2F2 acts in the nucleus as part of the Pol II transcription
        machinery. Nuclear localization is documented experimentally.
      action: ACCEPT
      reason: Nuclear localization is consistent with the protein's function in
        Pol II transcription and is independently supported by experimental IDA
        annotations; the broad nucleus term is appropriate even though the more
        specific nucleoplasm term also applies.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Subcellular localization Nuclear, chromatin-associated;
            localized at core promoters within the PIC and detectable in early
            elongation complexes
  - term:
      id: GO:0005674
      label: transcription factor TFIIF complex
    evidence_type: IEA
    original_reference_id: GO_REF:0000002
    review:
      summary: InterPro-based assignment of TFIIF complex membership, redundant
        with the IBA and IPI annotations to the same term.
      action: ACCEPT
      reason: Correct complex membership for the TFIIF beta subunit (InterPro
        IPR003196 = TFIIF_beta); this duplicates the experimentally and
        phylogenetically supported GO:0005674 annotations and is at the right
        level of specificity.
      supported_by:
        - reference_id: PMID:11183778
          supporting_text: it consists minimally of a heterodimer of RNA
            polymerase-associated proteins RAP30 and RAP74.
  - term:
      id: GO:0006366
      label: transcription by RNA polymerase II
    evidence_type: IEA
    original_reference_id: GO_REF:0000002
    review:
      summary: InterPro-based assignment to the general Pol II transcription
        process. GTF2F2 is a core general transcription factor for Pol II.
      action: ACCEPT
      reason: This is a correct but broad parent process; it is consistent with
        the more specific initiation and elongation annotations and is supported by
        experimental IMP and TAS annotations to the same term.
      supported_by:
        - reference_id: PMID:7929273
          supporting_text: TFIIF has been shown to bind RNA polymerase II and
            control the activity of the enzyme in both the initiation and
            elongation stages of transcription.
  - term:
      id: GO:0006367
      label: transcription initiation at RNA polymerase II promoter
    evidence_type: IEA
    original_reference_id: GO_REF:0000120
    review:
      summary: Combined-IEA assignment to Pol II transcription initiation, the core
        function of TFIIF, redundant with the IBA/ISS/IDA annotations to the same
        term.
      action: ACCEPT
      reason: Correct and well-supported core process; duplicates experimentally
        supported annotations to GO:0006367.
      supported_by:
        - reference_id: PMID:7929273
          supporting_text: both RAP30 and RAP74 function in synthesis of the first
            few phosphodiester bonds of nascent transcripts and in formation of
            Sarkosyl-resistant pre-initiation intermediates.
  - term:
      id: GO:0006368
      label: transcription elongation by RNA polymerase II
    evidence_type: IEA
    original_reference_id: GO_REF:0000117
    review:
      summary: ARBA machine-learning assignment to Pol II elongation. TFIIF
        stimulates the rate of Pol II elongation and suppresses transient pausing.
      action: ACCEPT
      reason: Correct process supported by direct biochemistry; redundant with
        IDA (PMID:15351637) and TAS (PMID:7929273) annotations to the same term.
      supported_by:
        - reference_id: PMID:7929273
          supporting_text: kinetic experiments indicate that both RAP30 and RAP74
            function in TFIIF-mediated stimulation of the rate of RNA chain
            elongation by RNA polymerase II.
  - term:
      id: GO:0016591
      label: RNA polymerase II, holoenzyme
    evidence_type: IEA
    original_reference_id: GO_REF:0000117
    review:
      summary: ARBA assignment placing GTF2F2 as part of the Pol II holoenzyme.
        TFIIF associates with Pol II as part of the preinitiation complex.
      action: KEEP_AS_NON_CORE
      reason: The protein does associate with Pol II within the preinitiation
        complex, so the annotation is not wrong, but the precise and preferred
        complex annotation for GTF2F2 is the TFIIF complex (GO:0005674). The
        holoenzyme term is a broader/looser localization that is acceptable but not
        the core complex membership.
      supported_by:
        - reference_id: PMID:7929273
          supporting_text: TFIIF has been shown to bind RNA polymerase II and
            control the activity of the enzyme in both the initiation and
            elongation stages of transcription.
  - term:
      id: GO:0090575
      label: RNA polymerase II transcription regulator complex
    evidence_type: IEA
    original_reference_id: GO_REF:0000117
    review:
      summary: ARBA assignment to the broad Pol II transcription regulator complex
        category.
      action: KEEP_AS_NON_CORE
      reason: GTF2F2 is part of a Pol II-associated transcription complex, so this
        broad parent grouping is defensible, but the specific and preferred complex
        annotation is the TFIIF complex (GO:0005674). Retained as non-core because
        it is less informative than the specific complex term.
      supported_by:
        - reference_id: PMID:11183778
          supporting_text: General transcription factor IIF (TFIIF) is required for
            transcription by RNA polymerase II
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:11183778
    review:
      summary: This IPI is the RAP30-RAP74 (GTF2F1) dimerization interaction
        determined by X-ray crystallography of the TFIIF interaction domains. This
        is the defining heterodimer interaction of the protein.
      action: MODIFY
      reason: Bare protein binding is uninformative. The interactor is the partner
        subunit GTF2F1/RAP74 and the relationship is TFIIF complex formation;
        GO:0044877 (protein-containing complex binding) is more informative and
        captures that RAP30 binds within the TFIIF complex. The complex membership
        itself is already captured by the GO:0005674 annotation.
      proposed_replacement_terms:
        - id: GO:0044877
          label: protein-containing complex binding
      supported_by:
        - reference_id: PMID:11183778
          supporting_text: The X-ray structure of the RAP30/RAP74 interaction
            domains at 1.7 A resolution reveals a novel "triple barrel"
            dimerization fold
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:12737519
    review:
      summary: IPI capturing interaction of RAP30/TFIIF with URI1 (RMP), an RPB5-
        mediating corepressor that binds both RAP30 and RAP74 to suppress activated
        transcription.
      action: ACCEPT
      reason: This is a specific, experimentally validated protein-protein
        interaction (pull-down, Far-Western, co-IP) with a defined biological
        consequence. Although the term is the generic protein binding, the
        interaction is real and documented; retaining as a binding annotation is
        acceptable. The functional context (transcriptional repression via TFIIF)
        is informative.
      supported_by:
        - reference_id: PMID:12737519
          supporting_text: we demonstrated that RMP could bind with bacterially
            expressed recombinant RAP30 and RAP74 of TFIIF subunits.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:20195357
    review:
      summary: High-throughput interaction screen (IVV/mRNA-display based mapping
        of transcription factor networks) reporting interactions of GTF2F2,
        including with URI1 and RPL27A.
      action: KEEP_AS_NON_CORE
      reason: This is a generic protein binding annotation from a large-scale
        interactome resource. It does not identify a specific informative molecular
        function and the individual interactions are not independently validated
        here. Retained as non-core supporting evidence rather than removed, since
        TFIIF is genuinely a hub in transcription factor networks.
      supported_by:
        - reference_id: PMID:20195357
          supporting_text: A comprehensive resource of interacting protein regions
            for refining human transcription factor networks.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:24981860
    review:
      summary: Affinity-purification/MS interactome study of chromatin-related
        proteins reporting an interaction involving GTF2F2 (with GTF2F1/RAP74).
      action: KEEP_AS_NON_CORE
      reason: Generic protein binding from a high-throughput chromatin interactome
        screen. The partner (RAP74) is the expected TFIIF dimer partner, so the hit
        is biologically plausible, but the term itself is uninformative. Kept as
        non-core supporting evidence.
      supported_by:
        - reference_id: PMID:24981860
          supporting_text: Human-chromatin-related protein interactions identify a
            demethylase complex required for chromosome segregation.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:31467278
    review:
      summary: Binary interactome mapping methodology study reporting a GTF2F2
        interaction with GTF2F1/RAP74.
      action: KEEP_AS_NON_CORE
      reason: Generic protein binding derived from a high-throughput binary
        interactome assay; the recovered partner is the canonical TFIIF dimer
        partner. Not informative as a molecular function term, retained as non-core
        corroboration of the RAP30-RAP74 interaction.
      supported_by:
        - reference_id: PMID:31467278
          supporting_text: Maximizing binary interactome mapping with a minimal
            number of assays.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:32296183
    review:
      summary: HuRI reference binary interactome reporting GTF2F2 interactions
        (e.g., FHL2, ZMYND19, MAPRE3).
      action: KEEP_AS_NON_CORE
      reason: Generic protein binding from a systematic binary interactome map.
        These hits are not individually validated and do not specify an
        informative molecular function. Some (e.g., MAPRE3/EB3) may underlie the
        spurious microtubule-cytoskeleton localization. Retained as non-core.
      supported_by:
        - reference_id: PMID:32296183
          supporting_text: A reference map of the human binary protein interactome.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:37398436
    review:
      summary: AI-guided PPI drug-discovery pipeline (binary interactome platform)
        reporting an interaction involving GTF2F2 and GTF2F1/RAP74.
      action: KEEP_AS_NON_CORE
      reason: Generic protein binding from a high-throughput interaction platform;
        the partner is the expected TFIIF dimer subunit. Uninformative as a
        molecular function term, retained as non-core supporting data.
      supported_by:
        - reference_id: PMID:37398436
          supporting_text: AI-guided pipeline for protein-protein interaction drug
            discovery identifies a SARS-CoV-2 inhibitor.
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:7854423
    review:
      summary: This annotation derives from a study showing serum response factor
        (SRF) binds the RAP74 subunit of TFIIF; the IntAct WITH field records the
        interaction against GTF2F1/RAP74 (P35269), i.e. RAP30's dimer partner.
      action: KEEP_AS_NON_CORE
      reason: The primary finding (SRF activation domain binding RAP74) concerns
        GTF2F1 rather than a direct RAP30 contact, and the annotation here is the
        generic protein binding term with RAP74 as the recorded partner. The
        RAP30-RAP74 dimerization is already better captured elsewhere; retained as
        non-core because it does not define an informative RAP30 molecular
        function.
      supported_by:
        - reference_id: PMID:7854423
          supporting_text: we find that SRF binds the RAP74 subunit of TFIIF and
            that SRF's transcriptional activation domain is the region involved in
            this binding.
  - term:
      id: GO:0005674
      label: transcription factor TFIIF complex
    evidence_type: IPI
    original_reference_id: PMID:11183778
    review:
      summary: ComplexPortal-curated TFIIF complex membership (CPX-79) for RAP30,
        based on the crystal structure of the RAP30/RAP74 heterodimer.
      action: ACCEPT
      reason: Correct, experimentally grounded complex membership at the right
        level of specificity. This is the preferred complex annotation for GTF2F2.
      supported_by:
        - reference_id: PMID:11183778
          supporting_text: it consists minimally of a heterodimer of RNA
            polymerase-associated proteins RAP30 and RAP74.
  - term:
      id: GO:0006367
      label: transcription initiation at RNA polymerase II promoter
    evidence_type: IDA
    original_reference_id: PMID:15351637
    review:
      summary: ComplexPortal IDA annotation of TFIIF to Pol II transcription
        initiation, based on transient-state kinetic dissection of the human Pol II
        mechanism in the presence of TFIIF.
      action: ACCEPT
      reason: Core process supported by direct in vitro biochemistry showing TFIIF
        acting on the Pol II initiation/early synthesis mechanism. Redundant with
        the IBA/IEA/ISS annotations to the same term but appropriately experimental.
      supported_by:
        - reference_id: PMID:15351637
          supporting_text: We report adequate two-bond kinetic simulations for the
            reaction in the presence of TFIIF alone and in the presence of
            TFIIF+TFIIS, providing detailed insight into the RNAP II mechanism
  - term:
      id: GO:0006368
      label: transcription elongation by RNA polymerase II
    evidence_type: IDA
    original_reference_id: PMID:15351637
    review:
      summary: ComplexPortal IDA annotation of TFIIF to Pol II elongation. The
        study shows TFIIF stabilizes the post-translocated elongation complex,
        supporting forward synthesis and suppressing pausing.
      action: ACCEPT
      reason: Directly supported by kinetic data showing TFIIF supports elongation
        and suppresses pausing by stabilizing the post-translocated elongation
        complex; this is a genuine core function of TFIIF.
      supported_by:
        - reference_id: PMID:15351637
          supporting_text: TFIIF supports elongation and suppresses pausing by
            stabilizing the post-translocated elongation complex.
  - term:
      id: GO:0045944
      label: positive regulation of transcription by RNA polymerase II
    evidence_type: IDA
    original_reference_id: PMID:15351637
    review:
      summary: ComplexPortal IDA annotation reflecting that TFIIF positively
        stimulates Pol II activity (forward synthesis and elongation rate).
      action: ACCEPT
      reason: TFIIF positively contributes to Pol II transcription output by
        promoting initiation and stimulating the elongation rate; the positive
        regulation term is supported by the functional data and by TFIIF's role in
        suppressing pausing/abortive cycles.
      supported_by:
        - reference_id: PMID:7929273
          supporting_text: both RAP30 and RAP74 function in TFIIF-mediated
            stimulation of the rate of RNA chain elongation by RNA polymerase II.
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: IDA
    original_reference_id: GO_REF:0000052
    review:
      summary: HPA immunofluorescence localizes GTF2F2 to the nucleoplasm, the
        compartment where Pol II transcription occurs.
      action: ACCEPT
      reason: Nucleoplasmic localization is the precise and expected compartment
        for a Pol II general transcription factor and is supported by curated
        immunofluorescence; consistent with the broader nucleus annotations.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Subcellular localization Nuclear, chromatin-associated;
            localized at core promoters within the PIC
  - term:
      id: GO:0015630
      label: microtubule cytoskeleton
    evidence_type: IDA
    original_reference_id: GO_REF:0000052
    review:
      summary: HPA immunofluorescence reported a microtubule-cytoskeleton signal.
        This is inconsistent with the established biology of GTF2F2 as a nuclear
        Pol II general transcription factor.
      action: REMOVE
      reason: There is no biochemical, structural, or mechanistic support for a
        cytoskeletal role of RAP30/TFIIF, which functions in the nucleus within the
        Pol II preinitiation and elongation complexes. This single HPA
        immunofluorescence localization most likely reflects antibody
        cross-reactivity or staining artifact (possibly related to interactome hits
        such as MAPRE3/EB3) and should not be retained as a genuine localization.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0006366
      label: transcription by RNA polymerase II
    evidence_type: IMP
    original_reference_id: PMID:8662660
    review:
      summary: This UniProt IMP reflects mutational dissection of RAP30 functional
        domains, in which N- and C-terminal RAP30 sequences were shown to be
        required for accurate Pol II transcription, RAP74 binding and TFIIB
        binding.
      action: ACCEPT
      reason: Domain-mutant transcription assays directly demonstrate that RAP30
        sequences are required for accurate Pol II transcription, supporting the
        broad process term. Redundant with the IEA/TAS annotations to GO:0006366
        but experimentally grounded.
      supported_by:
        - reference_id: PMID:8662660
          supporting_text: Transcription assays indicate the importance of both N-
            and C-terminal sequences for RAP30 function.
  - term:
      id: GO:0006367
      label: transcription initiation at RNA polymerase II promoter
    evidence_type: ISS
    original_reference_id: GO_REF:0000024
    review:
      summary: ISS transfer from an ortholog (UniProtKB:Q01750) of the core TFIIF
        initiation function.
      action: ACCEPT
      reason: Sequence-similarity transfer of the well-established core process is
        appropriate and concordant with the IBA and experimental annotations to
        GO:0006367.
      supported_by:
        - reference_id: PMID:7929273
          supporting_text: both RAP30 and RAP74 contribute to the formation of
            stable preinitiation intermediates containing RNA polymerase II.
  - term:
      id: GO:0016251
      label: RNA polymerase II general transcription initiation factor activity
    evidence_type: ISS
    original_reference_id: GO_REF:0000024
    review:
      summary: ISS assignment of the molecular function general transcription
        initiation factor activity for Pol II, transferred from an ortholog. This
        is the most informative molecular-function term for GTF2F2/TFIIF.
      action: ACCEPT
      reason: This is the appropriate, specific molecular function for the protein
        and is well supported by direct biochemistry showing TFIIF is required for
        accurate Pol II initiation. It is preferred over the generic protein
        binding annotations as the representative MF.
      supported_by:
        - reference_id: PMID:11183778
          supporting_text: General transcription factor IIF (TFIIF) is required for
            transcription by RNA polymerase II
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:8662660
    review:
      summary: IPI capturing the direct RAP30-TFIIB (GTF2B) interaction; TFIIB
        binds an overlapping N-terminal region of RAP30, and this contact is part
        of preinitiation complex assembly.
      action: MODIFY
      reason: Bare protein binding is uninformative. The interactor is the general
        transcription factor TFIIB (GTF2B), so the more specific MF term
        GO:0001091 (RNA polymerase II general transcription initiation factor
        binding) better describes this contact within the basal machinery.
      proposed_replacement_terms:
        - id: GO:0001091
          label: RNA polymerase II general transcription initiation factor binding
      supported_by:
        - reference_id: PMID:8662660
          supporting_text: TFIIB binds to an overlapping region of RAP30, localized
            to amino acids 1-176 (amino acids 27-152 comprise a minimal binding
            region).
  - term:
      id: GO:0005515
      label: protein binding
    evidence_type: IPI
    original_reference_id: PMID:8504927
    review:
      summary: IPI capturing the interaction between TFIIB (GTF2B) and the small
        subunit of TFIIF (RAP30); the N-terminus of TFIIB (including its zinc
        finger) contacts RAP30.
      action: MODIFY
      reason: The partner is the general transcription factor TFIIB, so the
        specific term GO:0001091 (RNA polymerase II general transcription
        initiation factor binding) is more informative than bare protein binding
        and reflects the role of this contact in PIC formation.
      proposed_replacement_terms:
        - id: GO:0001091
          label: RNA polymerase II general transcription initiation factor binding
      supported_by:
        - reference_id: PMID:8504927
          supporting_text: The interaction with the small subunit of TFIIF was
            mapped to the amino terminus of TFIIB, which includes a zinc finger.
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: IDA
    original_reference_id: PMID:9841876
    review:
      summary: CAFA-curated IDA nuclear localization, from a study of TFIIB/TFIIF
        recruitment during estrogen-receptor-facilitated preinitiation complex
        assembly.
      action: ACCEPT
      reason: Nuclear localization is correct and expected for a Pol II general
        transcription factor; concordant with the IEA, HDA, and IDA nucleoplasm
        annotations.
      supported_by:
        - reference_id: PMID:9841876
          supporting_text: recombinant human ER increased the stable association of
            subsequent components of the transcription machinery (TFIIE and TFIIF)
  - term:
      id: GO:0005634
      label: nucleus
    evidence_type: HDA
    original_reference_id: PMID:16791210
    review:
      summary: High-throughput dynamic-proteomics study classifying GTF2F2 as a
        nuclear protein.
      action: ACCEPT
      reason: Consistent with the established nuclear localization of the protein;
        supports the nucleus/nucleoplasm annotations even though derived from a
        high-throughput dataset.
      supported_by:
        - reference_id: PMID:16791210
          supporting_text: Dynamic proteomics in individual human cells uncovers
            widespread cell-cycle dependence of nuclear proteins.
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-109638
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-109639
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-111264
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-112379
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-112381
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-112383
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-112385
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-112392
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-112395
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-112396
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-113402
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-113407
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-113409
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-113411
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-113412
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-113413
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-113414
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-113429
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-113430
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-170704
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-170706
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6797606
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6797616
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6803523
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6803527
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6803836
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6803838
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6810233
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6810234
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6810235
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6810238
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6814549
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6814555
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6814559
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-6814885
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-72095
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-72103
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-73946
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-75079
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-75080
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-75081
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-75082
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-75083
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-75095
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-75850
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-75856
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-75861
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-75862
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-75864
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-75866
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-75869
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-75873
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-75891
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-75949
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-76576
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-77068
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-77069
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-77071
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-77073
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-77077
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-77078
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-77081
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-77083
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-77085
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-77090
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-77095
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9012315
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9012319
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9613494
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9613497
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9770119
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9770129
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9770131
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9770132
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9770141
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9770142
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9770145
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9770236
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9770847
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0005654
      label: nucleoplasm
    evidence_type: TAS
    original_reference_id: Reactome:R-HSA-9794542
    review:
      summary: GTF2F2 is placed in the nucleoplasm as a participant in Reactome
        reactions covering Pol II preinitiation, initiation, promoter escape,
        elongation, capping, splicing and related steps. The localization is
        correct; the many reaction-specific references reflect Reactome pathway
        granularity rather than distinct localizations.
      action: ACCEPT
      reason: Nucleoplasm is the correct compartment for a Pol II general
        transcription factor and is independently supported by HPA immunofluorescence
        (IDA) and experimental nucleus annotations. These TAS entries are redundant
        with each other but not incorrect; some downstream reactions (e.g. splicing,
        capping) reflect pathway membership of the broader machinery rather than a
        direct GTF2F2 role, but the nucleoplasm localization itself is accurate.
      supported_by:
        - reference_id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
          supporting_text: Nuclear, chromatin-associated; localized at core
            promoters within the PIC and detectable in early elongation complexes
  - term:
      id: GO:0006366
      label: transcription by RNA polymerase II
    evidence_type: TAS
    original_reference_id: PMID:7929273
    review:
      summary: TAS annotation to Pol II transcription, from a study establishing
        that both RAP30 and RAP74 contribute to Pol II transcription initiation and
        elongation.
      action: ACCEPT
      reason: Author-stated, experimentally grounded assignment of the core process;
        redundant with the IMP and IEA annotations to GO:0006366.
      supported_by:
        - reference_id: PMID:7929273
          supporting_text: Roles for both the RAP30 and RAP74 subunits of 
            transcription factor IIF in transcription initiation and elongation 
            by RNA polymerase II.
  - term:
      id: GO:0006368
      label: transcription elongation by RNA polymerase II
    evidence_type: TAS
    original_reference_id: PMID:7929273
    review:
      summary: TAS annotation to Pol II elongation; the cited study shows both
        RAP30 and RAP74 function in TFIIF-mediated stimulation of the Pol II
        elongation rate.
      action: ACCEPT
      reason: Author-stated, experimentally supported assignment of a genuine core
        function (elongation stimulation); concordant with IDA (PMID:15351637) and
        IEA annotations to GO:0006368.
      supported_by:
        - reference_id: PMID:7929273
          supporting_text: Roles for both the RAP30 and RAP74 subunits of 
            transcription factor IIF in transcription initiation and elongation 
            by RNA polymerase II.
references:
  - id: GO_REF:0000002
    title: Gene Ontology annotation through association of InterPro records with
      GO terms.
    findings: []
  - id: GO_REF:0000024
    title: Manual transfer of experimentally-verified manual GO annotation data 
      to orthologs by curator judgment of sequence similarity.
    findings: []
  - id: GO_REF:0000033
    title: Annotation inferences using phylogenetic trees
    findings: []
  - id: GO_REF:0000043
    title: Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword 
      mapping
    findings: []
  - id: GO_REF:0000044
    title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular 
      Location vocabulary mapping, accompanied by conservative changes to GO 
      terms applied by UniProt.
    findings: []
  - id: GO_REF:0000052
    title: Gene Ontology annotation based on curation of immunofluorescence data
    findings: []
  - id: GO_REF:0000117
    title: Electronic Gene Ontology annotations created by ARBA machine learning
      models
    findings: []
  - id: GO_REF:0000120
    title: Combined Automated Annotation using Multiple IEA Methods.
    findings: []
  - id: PMID:11183778
    title: Novel dimerization fold of RAP30/RAP74 in human TFIIF at 1.7 A 
      resolution.
    findings: []
  - id: PMID:12737519
    title: Interaction with general transcription factor IIF (TFIIF) is required
      for the suppression of activated transcription by RPB5-mediating protein 
      (RMP).
    findings: []
  - id: PMID:15351637
    title: Transcription factors IIF and IIS and nucleoside triphosphate 
      substrates as dynamic probes of the human RNA polymerase II mechanism.
    findings: []
  - id: PMID:16791210
    title: Dynamic proteomics in individual human cells uncovers widespread 
      cell-cycle dependence of nuclear proteins.
    findings: []
  - id: PMID:20195357
    title: A comprehensive resource of interacting protein regions for refining 
      human transcription factor networks.
    findings: []
  - id: PMID:24981860
    title: Human-chromatin-related protein interactions identify a demethylase 
      complex required for chromosome segregation.
    findings: []
  - id: PMID:31467278
    title: Maximizing binary interactome mapping with a minimal number of 
      assays.
    findings: []
  - id: PMID:32296183
    title: A reference map of the human binary protein interactome.
    findings: []
  - id: PMID:37398436
    title: AI-guided pipeline for protein-protein interaction drug discovery 
      identifies a SARS-CoV-2 inhibitor.
    findings: []
  - id: PMID:7854423
    title: Interaction with RAP74 subunit of TFIIF is required for 
      transcriptional activation by serum response factor.
    findings: []
  - id: PMID:7929273
    title: Roles for both the RAP30 and RAP74 subunits of transcription factor 
      IIF in transcription initiation and elongation by RNA polymerase II.
    findings: []
  - id: PMID:8504927
    title: 'Multiple functional domains of human transcription factor IIB: distinct
      interactions with two general transcription factors and RNA polymerase II.'
    findings: []
  - id: PMID:8662660
    title: RNA polymerase II-associated protein (RAP) 74 binds transcription 
      factor (TF) IIB and blocks TFIIB-RAP30 binding.
    findings: []
  - id: PMID:9841876
    title: 'Oestrogen receptor facilitates the formation of preinitiation complex
      assembly: involvement of the general transcription factor TFIIB.'
    findings: []
  - id: Reactome:R-HSA-109638
    title: Recruitment of RNA Polymerase II Holoenzyme by TFIIF to the pol II 
      promoter:TFIID:TFIIA:TFIIB complex
    findings: []
  - id: Reactome:R-HSA-109639
    title: Formation of the closed pre-initiation complex
    findings: []
  - id: Reactome:R-HSA-111264
    title: Addition of nucleotides between position +11 and +30
    findings: []
  - id: Reactome:R-HSA-112379
    title: Recruitment of elongation factors to form elongation complex
    findings: []
  - id: Reactome:R-HSA-112381
    title: Hyperphosphorylation (Ser2) of RNA Pol II CTD by P-TEFb complex
    findings: []
  - id: Reactome:R-HSA-112383
    title: Hypophosphorylation of RNA Pol II CTD by FCP1P protein
    findings: []
  - id: Reactome:R-HSA-112385
    title: Addition of nucleotides leads to transcript elongation
    findings: []
  - id: Reactome:R-HSA-112392
    title: Resumption of elongation after recovery from pausing
    findings: []
  - id: Reactome:R-HSA-112395
    title: Abortive termination of elongation after arrest
    findings: []
  - id: Reactome:R-HSA-112396
    title: Separation of elongating transcript from template
    findings: []
  - id: Reactome:R-HSA-113402
    title: Formation of DSIF:NELF:early elongation complex
    findings: []
  - id: Reactome:R-HSA-113407
    title: DSIF complex binds to RNA Pol II (hypophosphorylated)
    findings: []
  - id: Reactome:R-HSA-113409
    title: Abortive termination of early transcription elongation by DSIF:NELF
    findings: []
  - id: Reactome:R-HSA-113411
    title: 2-4 nt.backtracking of Pol II complex on the template leading to 
      elongation pausing
    findings: []
  - id: Reactome:R-HSA-113412
    title: Pol II elongation complex moves on the template as transcript 
      elongates
    findings: []
  - id: Reactome:R-HSA-113413
    title: TFIIS-mediated recovery of elongation from arrest
    findings: []
  - id: Reactome:R-HSA-113414
    title: 7-14 nt. Backtracking of Pol II complex on the template leading to 
      elongation arrest
    findings: []
  - id: Reactome:R-HSA-113429
    title: Elongating transcript encounters a lesion in the template
    findings: []
  - id: Reactome:R-HSA-113430
    title: Extrusion of 5'-end of 30 nt long transcript through the pore in Pol 
      II complex
    findings: []
  - id: Reactome:R-HSA-170704
    title: Phosphorylation of DSIF by the P-TEFb(Cyclin T1:Cdk9) complex
    findings: []
  - id: Reactome:R-HSA-170706
    title: Phosphorylation of  NEFL by the P-TEFb(Cyclin T1:Cdk9) complex
    findings: []
  - id: Reactome:R-HSA-6797606
    title: CDK12 phosphorylates RNA Pol II CTD at DNA repair genes
    findings: []
  - id: Reactome:R-HSA-6797616
    title: CCNK:CDK12 binds RNA Pol II at DNA repair genes
    findings: []
  - id: Reactome:R-HSA-6803523
    title: PTB and hnRNPA1 bind FGFR2 pre-mRNA to repress IIIb splicing and 
      promote formation of FGFR2c mRNA
    findings: []
  - id: Reactome:R-HSA-6803527
    title: ESRP1 and 2 bind FGFR2 pre-mRNA to promote FGFR2b maturation and 
      expression
    findings: []
  - id: Reactome:R-HSA-6803836
    title: FGFR2b-specific alternative splicing produces FGFR2b transcript
    findings: []
  - id: Reactome:R-HSA-6803838
    title: FGFR2c-specific alternative splicing produces FGFR2c transcript
    findings: []
  - id: Reactome:R-HSA-6810233
    title: CDK7 phosphorylates serine-5 and serine-7 of heptad repeats in 
      C-terminal domain of RNA polymerase II at snRNA promoter
    findings: []
  - id: Reactome:R-HSA-6810234
    title: General transcription factors bind SNAPc:POU2F1:ZNF143:snRNA gene
    findings: []
  - id: Reactome:R-HSA-6810235
    title: RPAP2 binds RNA polymerase II phosphorylated at serine-7 residues of 
      heptad repeats in the C-terminal domain
    findings: []
  - id: Reactome:R-HSA-6810238
    title: RNA polymerase II binds initiation factors at promoter of snRNA gene 
      (U1, U2, U4, U4atac, U5, U11, U12)
    findings: []
  - id: Reactome:R-HSA-6814549
    title: Pre-snRNA transcript initiation, Integrator binding, LEC binding
    findings: []
  - id: Reactome:R-HSA-6814555
    title: Integrator complex processes the 3' end of snRNA
    findings: []
  - id: Reactome:R-HSA-6814559
    title: Pre-snRNA is elongated and capped with 7-methylguanosine
    findings: []
  - id: Reactome:R-HSA-6814885
    title: CBCAP complex binds 7-methylguanosine cap of snRNA
    findings: []
  - id: Reactome:R-HSA-72095
    title: Internal Methylation of mRNA
    findings: []
  - id: Reactome:R-HSA-72103
    title: Formation of pre-mRNPs
    findings: []
  - id: Reactome:R-HSA-73946
    title: Abortive initiation after formation of the first phosphodiester bond
    findings: []
  - id: Reactome:R-HSA-75079
    title: Formation of AT-AC C complex
    findings: []
  - id: Reactome:R-HSA-75080
    title: Formation of AT-AC A complex
    findings: []
  - id: Reactome:R-HSA-75081
    title: Formation of AT-AC B Complex
    findings: []
  - id: Reactome:R-HSA-75082
    title: ATAC spliceosome mediated Lariat formation,5' splice site cleavage
    findings: []
  - id: Reactome:R-HSA-75083
    title: ATAC spliceosome mediated 3' splice site cleavage, exon ligation
    findings: []
  - id: Reactome:R-HSA-75095
    title: Binding of TFIIE to the growing preinitiation complex
    findings: []
  - id: Reactome:R-HSA-75850
    title: Addition of the third nucleotide on the nascent transcript
    findings: []
  - id: Reactome:R-HSA-75856
    title: Abortive Initiation Before Second Transition
    findings: []
  - id: Reactome:R-HSA-75861
    title: NTP Binds Active Site of RNA Polymerase II
    findings: []
  - id: Reactome:R-HSA-75862
    title: Fall Back to Closed Pre-initiation Complex
    findings: []
  - id: Reactome:R-HSA-75864
    title: Newly Formed Phosphodiester Bond Stabilized and PPi Released
    findings: []
  - id: Reactome:R-HSA-75866
    title: Nucleophillic Attack by 3'-hydroxyl Oxygen of nascent transcript on 
      the Alpha Phosphate of NTP
    findings: []
  - id: Reactome:R-HSA-75869
    title: 'Addition of the fourth nucleotide on the Nascent Transcript: Second Transition'
    findings: []
  - id: Reactome:R-HSA-75873
    title: Addition of Nucleotides 5 through 9 on the growing Transcript
    findings: []
  - id: Reactome:R-HSA-75891
    title: Abortive Initiation After Second Transition
    findings: []
  - id: Reactome:R-HSA-75949
    title: 'RNA Polymerase II Promoter Opening: First Transition'
    findings: []
  - id: Reactome:R-HSA-76576
    title: 'Addition of nucleotides 10 and 11 on the growing transcript: Third Transition'
    findings: []
  - id: Reactome:R-HSA-77068
    title: Activation of GT
    findings: []
  - id: Reactome:R-HSA-77069
    title: RNA Polymerase II CTD (phosphorylated) binds to CE
    findings: []
  - id: Reactome:R-HSA-77071
    title: Phosphorylation (Ser5) of RNA pol II CTD
    findings: []
  - id: Reactome:R-HSA-77073
    title: SPT5 subunit of Pol II binds the RNA triphosphatase (RTP)
    findings: []
  - id: Reactome:R-HSA-77077
    title: Capping complex formation
    findings: []
  - id: Reactome:R-HSA-77078
    title: Hydrolysis of the 5'-end of the nascent transcript by the capping 
      enzyme
    findings: []
  - id: Reactome:R-HSA-77081
    title: Formation of the CE:GMP intermediate complex
    findings: []
  - id: Reactome:R-HSA-77083
    title: Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA
    findings: []
  - id: Reactome:R-HSA-77085
    title: Dissociation of  transcript with 5'-GMP from GT
    findings: []
  - id: Reactome:R-HSA-77090
    title: Methylation of GMP-cap by RNA Methyltransferase
    findings: []
  - id: Reactome:R-HSA-77095
    title: 'Recognition and binding of the mRNA cap by the cap-binding complex '
    findings: []
  - id: Reactome:R-HSA-9012315
    title: ESR1:ESTG:P-TEFb recruited to paused RNA polymerase II on MYB gene
    findings: []
  - id: Reactome:R-HSA-9012319
    title: p-TEFb phosphorylates serine 2 in RNA polymerase II CTD
    findings: []
  - id: Reactome:R-HSA-9613494
    title: 'Unwinding of DNA for the Nascent Transcript: Second Transition'
    findings: []
  - id: Reactome:R-HSA-9613497
    title: Unwinding DNA for the nascent transcript
    findings: []
  - id: Reactome:R-HSA-9770119
    title: Formation of the Spliceosomal E complex
    findings: []
  - id: Reactome:R-HSA-9770129
    title: Formation of the Spliceosomal A complex
    findings: []
  - id: Reactome:R-HSA-9770131
    title: Formation of the Spliceosomal B* complex
    findings: []
  - id: Reactome:R-HSA-9770132
    title: Formation of the Spliceosomal Pre-B complex
    findings: []
  - id: Reactome:R-HSA-9770141
    title: Formation of the Spliceosomal C* complex
    findings: []
  - id: Reactome:R-HSA-9770142
    title: Formation of the Spliceosomal B complex
    findings: []
  - id: Reactome:R-HSA-9770145
    title: Formation of the Spliceosomal Bact complex
    findings: []
  - id: Reactome:R-HSA-9770236
    title: Formation of the Spliceosomal P complex and exon ligation
    findings: []
  - id: Reactome:R-HSA-9770847
    title: Spliceosomal P complex dissociates yielding the intron-containing 
      complex (ILS) and the spliced mRNP (new)
    findings: []
  - id: Reactome:R-HSA-9794542
    title: Formation of the Spliceosomal C complex containing intron lariat
    findings: []
  - id: file:human/GTF2F2/GTF2F2-deep-research-falcon.md
    title: Deep research report on GTF2F2
    findings: []
core_functions:
  - description: GTF2F2/RAP30 is the beta subunit of general transcription factor
      TFIIF, providing RNA polymerase II general transcription initiation factor
      activity. As part of the TFIIF heterodimer with GTF2F1/RAP74, it binds Pol II
      and helps recruit and stabilize it within the preinitiation complex assembled
      on promoter DNA by TBP/TFIID, TFIIA and TFIIB, while suppressing nonspecific
      Pol II-DNA binding.
    molecular_function:
      id: GO:0016251
      label: RNA polymerase II general transcription initiation factor activity
    directly_involved_in:
      - id: GO:0006367
        label: transcription initiation at RNA polymerase II promoter
    locations:
      - id: GO:0005654
        label: nucleoplasm
    in_complex:
      id: GO:0005674
      label: transcription factor TFIIF complex
    supported_by:
      - reference_id: PMID:7929273
        supporting_text: both RAP30 and RAP74 contribute to the formation of stable
          preinitiation intermediates containing RNA polymerase II.
      - reference_id: PMID:11183778
        supporting_text: General transcription factor IIF (TFIIF) is required for
          transcription by RNA polymerase II; it consists minimally of a heterodimer
          of RNA polymerase-associated proteins RAP30 and RAP74.
  - description: Within TFIIF, the RAP30 C-terminal winged-helix/HTH domain binds
      promoter DNA and, together with contacts to TFIIB, helps position the
      polymerase and stabilize promoter DNA during start-site selection and promoter
      opening (with TFIIE/TFIIH).
    molecular_function:
      id: GO:0001091
      label: RNA polymerase II general transcription initiation factor binding
    directly_involved_in:
      - id: GO:0006367
        label: transcription initiation at RNA polymerase II promoter
    locations:
      - id: GO:0005654
        label: nucleoplasm
    supported_by:
      - reference_id: PMID:8662660
        supporting_text: TFIIB binds to an overlapping region of RAP30, localized to
          amino acids 1-176 (amino acids 27-152 comprise a minimal binding region).
      - reference_id: PMID:11183778
        supporting_text: the multiple domains of RAP30 and RAP74 bind PolII, TFIIB,
          TAF250 and DNA in interactions that are essential for transcription
          initiation and elongation.
  - description: After initiation, TFIIF remains associated with Pol II during early
      transcription, where it stimulates the rate of RNA chain elongation and
      suppresses transient pausing/backtracking by stabilizing the post-translocated
      elongation complex, promoting the transition to productive elongation.
    directly_involved_in:
      - id: GO:0006368
        label: transcription elongation by RNA polymerase II
    locations:
      - id: GO:0005654
        label: nucleoplasm
    supported_by:
      - reference_id: PMID:7929273
        supporting_text: both RAP30 and RAP74 function in TFIIF-mediated stimulation
          of the rate of RNA chain elongation by RNA polymerase II.
      - reference_id: PMID:15351637
        supporting_text: TFIIF supports elongation and suppresses pausing by
          stabilizing the post-translocated elongation complex.
proposed_new_terms: []
suggested_questions:
  - question: Does the RAP30 winged-helix DNA-binding domain make sequence-nonspecific
      versus sequence-influenced contacts that contribute to transcription start-site
      selection in human promoters?
    experts:
      - Cramer P
      - Nogales E
  - question: Are the reported high-throughput interactome partners of GTF2F2 outside
      the core transcription machinery (e.g. FHL2, ZMYND19, MAPRE3) functionally
      meaningful, or do they represent assay artifacts?
    experts:
      - Vidal M
suggested_experiments:
  - hypothesis: The RAP30 C-terminal winged-helix domain is required for correct Pol II
      start-site selection and promoter-proximal positioning, distinct from its role
      in RAP74 dimerization.
    description: Reconstitute human Pol II transcription in vitro with wild-type versus
      winged-helix-domain mutant RAP30 and map transcription start sites and promoter
      DNA contacts (e.g. by primer extension and crosslinking/cryo-EM).
    experiment_type: in vitro reconstituted transcription and structural mapping
  - hypothesis: GTF2F2 is broadly required for Pol II transcription across most active
      promoters, with measurable effects on elongation rate and promoter-proximal
      pausing.
    description: Acutely deplete GTF2F2 (e.g. degron) in human cells and measure
      nascent transcription genome-wide (e.g. PRO-seq/TT-seq) to quantify effects on
      initiation, pause-release, and elongation rate.
    experiment_type: acute depletion with nascent transcription profiling