ILK (integrin-linked kinase; now annotated by UniProt as 'Scaffold protein ILK') is a focal-adhesion protein of the protein-kinase superfamily that is catalytically dead - a pseudokinase. Rather than phosphorylating substrates, it functions as the central scaffold of the heterotrimeric IPP (ILK-PINCH-PARVIN) complex, linking integrin cytoplasmic tails to the actin cytoskeleton and coordinating cell-matrix adhesion, spreading, and integrin/growth-factor signaling. Its pseudokinase domain still binds ATP/Mg but performs no catalysis. ILK is essential for focal-adhesion organization, actin filament assembly, and is implicated in cardiac/muscle function and cancer.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005737 cytoplasm | IBA GO_REF:0000033 | ACCEPT | Summary: cytoplasm: a core subcellular location for ILK. Reason: Correct core localization. |
| GO:0007229 integrin-mediated signaling pathway | IBA GO_REF:0000033 | ACCEPT | Summary: integrin-mediated signaling pathway: a core biological process for ILK. Reason: Core process consistent with ILK's established function. |
| GO:0005102 signaling receptor binding | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: signaling receptor binding. Reason: Retained as non-core. |
| GO:0030154 cell differentiation | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: cell differentiation: process annotation for ILK. Reason: Valid but non-core / secondary. |
| GO:0030674 protein-macromolecule adaptor activity | IBA GO_REF:0000033 | ACCEPT | Summary: Protein-macromolecule adaptor activity: ILK's actual molecular function as the scaffold of the IPP (ILK-PINCH-PARVIN) complex linking integrins to the actin cytoskeleton. Reason: Core molecular function for the pseudokinase scaffold. Retain. Supporting Evidence: file:human/ILK/ILK-uniprot.txt Scaffold protein which mediates protein-protein interactions |
| GO:0005925 focal adhesion | IBA GO_REF:0000033 | ACCEPT | Summary: focal adhesion: a core subcellular location for ILK. Reason: Correct core localization. |
| GO:0007160 cell-matrix adhesion | IBA GO_REF:0000033 | ACCEPT | Summary: cell-matrix adhesion: a core biological process for ILK. Reason: Core process consistent with ILK's established function. |
| GO:0034446 substrate adhesion-dependent cell spreading | IBA GO_REF:0000033 | ACCEPT | Summary: substrate adhesion-dependent cell spreading: a core biological process for ILK. Reason: Core process consistent with ILK's established function. |
| GO:0004672 protein kinase activity | IEA GO_REF:0000002 | REMOVE | Summary: Positive 'protein kinase activity' (IEA) propagated from the protein-kinase-superfamily (TKL) signature. ILK is a pseudokinase with a degenerate catalytic site and no kinase activity. Reason: Electronic over-propagation refuted on biological grounds: ILK is established as a catalytically dead scaffold (the curated NOT|ser/thr kinase). Remove. Supporting Evidence: file:human/ILK/ILK-uniprot.txt Now thought to be a pseudokinase which does not have kinase activity and which functions solely as a scaffold protein |
| GO:0005524 ATP binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: ATP binding: ILK's pseudokinase domain still binds ATP/Mg despite lacking catalytic activity (IDA). Reason: Real (structurally demonstrated) nucleotide binding but not catalytic; non-core for a scaffold. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: nucleus: a secondary/broad localization for ILK. Reason: Plausible but non-core (broad term or context-specific). |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | ACCEPT | Summary: cytoplasm: a core subcellular location for ILK. Reason: Correct core localization. |
| GO:0005813 centrosome | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: centrosome: a secondary/broad localization for ILK. Reason: Plausible but non-core (broad term or context-specific). |
| GO:0005886 plasma membrane | IEA GO_REF:0000044 | ACCEPT | Summary: plasma membrane: a core subcellular location for ILK. Reason: Correct core localization. |
| GO:0005925 focal adhesion | IEA GO_REF:0000120 | ACCEPT | Summary: focal adhesion: a core subcellular location for ILK. Reason: Correct core localization. |
| GO:0005938 cell cortex | IEA GO_REF:0000044 | ACCEPT | Summary: cell cortex: a core subcellular location for ILK. Reason: Correct core localization. |
| GO:0009653 anatomical structure morphogenesis | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: anatomical structure morphogenesis: process annotation for ILK. Reason: Valid but non-core / secondary. |
| GO:0030017 sarcomere | IEA GO_REF:0000044 | ACCEPT | Summary: sarcomere: a core subcellular location for ILK. Reason: Correct core localization. |
| GO:0030027 lamellipodium | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: lamellipodium: a secondary/broad localization for ILK. Reason: Plausible but non-core (broad term or context-specific). |
| GO:0005515 protein binding | IPI PMID:11331308 A new focal adhesion protein that interacts with integrin-li... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:11331308) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:11331582 Modulation of integrin signal transduction by ILKAP, a prote... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:11331582) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:12432066 Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is e... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:12432066) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:16189514) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:16936772 Integrin-linked kinase interacts with caspase-9 and -8 in an... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:16936772) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:17003112 Posttranslational hydroxylation of ankyrin repeats in Ikappa... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:17003112) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:17553790 Interaction of integrin-linked kinase with the kidney chlori... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:17553790) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:18339839 Rictor and integrin-linked kinase interact and regulate Akt ... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:18339839) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:19074270 The structural basis of integrin-linked kinase-PINCH interac... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:19074270) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:21516116 Next-generation sequencing to generate interactome datasets. | MARK AS OVER ANNOTATED | Summary: Generic 'protein binding' from a high-throughput interactome screen (PMID:21516116). Reason: High-throughput protein binding is uninformative about molecular function (curation guideline). Over-annotation. |
| GO:0005515 protein binding | IPI PMID:21643011 Phosphorylation and interaction of myopodin by integrin-link... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:21643011) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:22939624 Quantitative analysis of HSP90-client interactions reveals p... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:22939624) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:23414517 A human skeletal muscle interactome centered on proteins inv... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:23414517) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:23455922 Interlaboratory reproducibility of large-scale human protein... | MARK AS OVER ANNOTATED | Summary: Specific protein interaction (PMID:23455922) consistent with ILK's scaffold/adapter role. Reason: Large-scale standardized AP-MS protein-complex study; high-throughput generic protein binding, grouped with the other HT-screen over-annotations for consistency. |
| GO:0005515 protein binding | IPI PMID:23877428 Interaction of smoothened with integrin-linked kinase in pri... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:23877428) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: Generic 'protein binding' from a high-throughput interactome screen (PMID:25416956). Reason: High-throughput protein binding is uninformative about molecular function (curation guideline). Over-annotation. |
| GO:0005515 protein binding | IPI PMID:25502805 A massively parallel pipeline to clone DNA variants and exam... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:25502805) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:25852190 Integrative analysis of kinase networks in TRAIL-induced apo... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:25852190) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:26496610 A human interactome in three quantitative dimensions organiz... | MARK AS OVER ANNOTATED | Summary: Specific protein interaction (PMID:26496610) consistent with ILK's scaffold/adapter role. Reason: Large-scale quantitative interactome study ('A human interactome in three quantitative dimensions'); generic protein binding from a high-throughput screen is uninformative about molecular function. Grouped with the other HT-screen over-annotations. |
| GO:0005515 protein binding | IPI PMID:26972000 Substrate-Trapped Interactors of PHD3 and FIH Cluster in Dis... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:26972000) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | MARK AS OVER ANNOTATED | Summary: Generic 'protein binding' from a high-throughput interactome screen (PMID:28514442). Reason: High-throughput protein binding is uninformative about molecular function (curation guideline). Over-annotation. |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: Generic 'protein binding' from a high-throughput interactome screen (PMID:31515488). Reason: High-throughput protein binding is uninformative about molecular function (curation guideline). Over-annotation. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: Generic 'protein binding' from a high-throughput interactome screen (PMID:32296183). Reason: High-throughput protein binding is uninformative about molecular function (curation guideline). Over-annotation. |
| GO:0005515 protein binding | IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:32707033) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MARK AS OVER ANNOTATED | Summary: Generic 'protein binding' from a high-throughput interactome screen (PMID:33961781). Reason: High-throughput protein binding is uninformative about molecular function (curation guideline). Over-annotation. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:35271311) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:36931259 A central chaperone-like role for 14-3-3 proteins in human c... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:36931259) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005515 protein binding | IPI PMID:40205054 Multimodal cell maps as a foundation for structural and func... | MARK AS OVER ANNOTATED | Summary: Generic 'protein binding' from a high-throughput interactome screen (PMID:40205054). Reason: High-throughput protein binding is uninformative about molecular function (curation guideline). Over-annotation. |
| GO:0003151 outflow tract morphogenesis | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: outflow tract morphogenesis: process annotation for ILK. Reason: Valid but non-core / secondary. |
| GO:0007229 integrin-mediated signaling pathway | IEA GO_REF:0000120 | ACCEPT | Summary: integrin-mediated signaling pathway: a core biological process for ILK. Reason: Core process consistent with ILK's established function. |
| GO:0030513 positive regulation of BMP signaling pathway | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: positive regulation of BMP signaling pathway: process annotation for ILK. Reason: Valid but non-core / secondary. |
| GO:0045669 positive regulation of osteoblast differentiation | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: positive regulation of osteoblast differentiation: process annotation for ILK. Reason: Valid but non-core / secondary. |
| GO:0070836 caveola assembly | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: caveola assembly: process annotation for ILK. Reason: Valid but non-core / secondary. |
| GO:0072697 protein localization to cell cortex | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: protein localization to cell cortex: process annotation for ILK. Reason: ILK's primary site is focal adhesions, not the broad cell cortex; keep as non-core for consistency with the caveola assembly annotation. |
| GO:0005925 focal adhesion | NAS PMID:35089438 Integrin-linked kinase (ILK): the known vs. the unknown and ... | ACCEPT | Summary: focal adhesion: a core subcellular location for ILK. Reason: Correct core localization. |
| GO:0051017 actin filament bundle assembly | IDA PMID:30367047 Non-catalytic signaling by pseudokinase ILK for regulating c... | KEEP AS NON CORE | Summary: actin filament bundle assembly: process annotation for ILK. Reason: Valid but non-core / secondary. |
| GO:0051639 actin filament network formation | IDA PMID:30367047 Non-catalytic signaling by pseudokinase ILK for regulating c... | KEEP AS NON CORE | Summary: actin filament network formation: process annotation for ILK. Reason: Valid but non-core / secondary. |
| GO:0005829 cytosol | IDA GO_REF:0000052 | ACCEPT | Summary: cytosol: a core subcellular location for ILK. Reason: Correct core localization. |
| GO:0005925 focal adhesion | IDA GO_REF:0000052 | ACCEPT | Summary: focal adhesion: a core subcellular location for ILK. Reason: Correct core localization. |
| GO:0005886 plasma membrane | EXP PMID:11402068 A novel integrin-linked kinase-binding protein, affixin, is ... | ACCEPT | Summary: plasma membrane: a core subcellular location for ILK. Reason: Correct core localization. |
| GO:0007229 integrin-mediated signaling pathway | ISS PMID:20675382 CYR61 regulates BMP-2-dependent osteoblast differentiation t... | ACCEPT | Summary: integrin-mediated signaling pathway: a core biological process for ILK. Reason: Core process consistent with ILK's established function. |
| GO:0000785 chromatin | IDA PMID:17420447 Phosphorylation-dependent regulation of nuclear localization... | KEEP AS NON CORE | Summary: chromatin: a secondary/broad localization for ILK. Reason: Plausible but non-core (broad term or context-specific). |
| GO:0005634 nucleus | IDA PMID:17420447 Phosphorylation-dependent regulation of nuclear localization... | KEEP AS NON CORE | Summary: nucleus: a secondary/broad localization for ILK. Reason: Plausible but non-core (broad term or context-specific). |
| GO:0005737 cytoplasm | IDA PMID:17420447 Phosphorylation-dependent regulation of nuclear localization... | ACCEPT | Summary: cytoplasm: a core subcellular location for ILK. Reason: Correct core localization. |
| GO:0005813 centrosome | IDA PMID:18283114 Integrin-linked kinase localizes to the centrosome and regul... | KEEP AS NON CORE | Summary: centrosome: a secondary/broad localization for ILK. Reason: Plausible but non-core (broad term or context-specific). |
| GO:0007052 mitotic spindle organization | IMP PMID:18283114 Integrin-linked kinase localizes to the centrosome and regul... | KEEP AS NON CORE | Summary: mitotic spindle organization: process annotation for ILK. Reason: Valid but non-core / secondary. |
| GO:0000287 magnesium ion binding | IDA PMID:20005845 The pseudoactive site of ILK is essential for its binding to... | KEEP AS NON CORE | Summary: Magnesium ion binding (IDA) by the pseudokinase domain, associated with ATP binding. Reason: Real but non-core (no catalytic role). |
| GO:0004674 protein serine/threonine kinase activity | IDA NOT PMID:20005845 The pseudoactive site of ILK is essential for its binding to... | ACCEPT | Summary: NOT: ILK does not have protein serine/threonine kinase activity. Structural and biochemical work established it as a pseudokinase scaffold (UniProt now names it 'Scaffold protein ILK'). Reason: Correct, important negation supported by direct evidence (IDA). Retain. Supporting Evidence: file:human/ILK/ILK-uniprot.txt Now thought to be a pseudokinase which does not have kinase activity and which functions solely as a scaffold protein |
| GO:0004674 protein serine/threonine kinase activity | IDA NOT PMID:21524996 Biochemical, proteomic, structural, and thermodynamic charac... | ACCEPT | Summary: NOT: ILK does not have protein serine/threonine kinase activity. Structural and biochemical work established it as a pseudokinase scaffold (UniProt now names it 'Scaffold protein ILK'). Reason: Correct, important negation supported by direct evidence (IDA). Retain. Supporting Evidence: file:human/ILK/ILK-uniprot.txt Now thought to be a pseudokinase which does not have kinase activity and which functions solely as a scaffold protein |
| GO:0005178 integrin binding | IDA PMID:20005845 The pseudoactive site of ILK is essential for its binding to... | ACCEPT | Summary: Integrin binding (IDA): ILK binds integrin beta cytoplasmic tails, central to its focal-adhesion scaffold role. Reason: Core, experimentally supported interaction defining ILK's adhesion function. |
| GO:0005515 protein binding | IPI PMID:20005845 The pseudoactive site of ILK is essential for its binding to... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:20005845) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005524 ATP binding | IDA PMID:20005845 The pseudoactive site of ILK is essential for its binding to... | KEEP AS NON CORE | Summary: ATP binding: ILK's pseudokinase domain still binds ATP/Mg despite lacking catalytic activity (IDA). Reason: Real (structurally demonstrated) nucleotide binding but not catalytic; non-core for a scaffold. |
| GO:0005524 ATP binding | IDA PMID:21524996 Biochemical, proteomic, structural, and thermodynamic charac... | KEEP AS NON CORE | Summary: ATP binding: ILK's pseudokinase domain still binds ATP/Mg despite lacking catalytic activity (IDA). Reason: Real (structurally demonstrated) nucleotide binding but not catalytic; non-core for a scaffold. |
| GO:0043123 positive regulation of canonical NF-kappaB signal transduction | IMP PMID:21343177 Thymosin beta4 inhibits TNF-alpha-induced NF-kappaB activati... | KEEP AS NON CORE | Summary: positive regulation of canonical NF-kappaB signal transduction: process annotation for ILK. Reason: Valid but non-core / secondary. |
| GO:1900026 positive regulation of substrate adhesion-dependent cell spreading | IGI PMID:30254023 Kindlin-2 interacts with a highly conserved surface of ILK t... | ACCEPT | Summary: positive regulation of substrate adhesion-dependent cell spreading: a core biological process for ILK. Reason: Core process consistent with ILK's established function. |
| GO:0000902 cell morphogenesis | IMP PMID:30254023 Kindlin-2 interacts with a highly conserved surface of ILK t... | KEEP AS NON CORE | Summary: cell morphogenesis: process annotation for ILK. Reason: Valid but non-core / secondary. |
| GO:0005515 protein binding | IPI PMID:30254023 Kindlin-2 interacts with a highly conserved surface of ILK t... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:30254023) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0005925 focal adhesion | IDA PMID:30254023 Kindlin-2 interacts with a highly conserved surface of ILK t... | ACCEPT | Summary: focal adhesion: a core subcellular location for ILK. Reason: Correct core localization. |
| GO:0005515 protein binding | IPI PMID:15817463 Actopaxin interacts with TESK1 to regulate cell spreading on... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:15817463) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0033209 tumor necrosis factor-mediated signaling pathway | IMP PMID:21343177 Thymosin beta4 inhibits TNF-alpha-induced NF-kappaB activati... | KEEP AS NON CORE | Summary: tumor necrosis factor-mediated signaling pathway: process annotation for ILK. Reason: Valid but non-core / secondary. |
| GO:0009967 positive regulation of signal transduction | IMP PMID:10637513 Integrin-linked kinase regulates phosphorylation of serine 4... | KEEP AS NON CORE | Summary: positive regulation of signal transduction: process annotation for ILK. Reason: Valid but non-core / secondary. |
| GO:0005515 protein binding | IPI PMID:20926685 The roles of two distinct regions of PINCH-1 in the regulati... | KEEP AS NON CORE | Summary: Specific protein interaction (PMID:20926685) consistent with ILK's scaffold/adapter role. Reason: Real interaction kept as non-core supporting evidence; the generic 'protein binding' term is itself uninformative. |
| GO:0070527 platelet aggregation | HMP PMID:23382103 Platelet proteome analysis reveals integrin-dependent aggreg... | KEEP AS NON CORE | Summary: platelet aggregation: process annotation for ILK. Reason: Valid but non-core / secondary. |
| GO:0005925 focal adhesion | HDA PMID:21423176 Analysis of the myosin-II-responsive focal adhesion proteome... | ACCEPT | Summary: focal adhesion: a core subcellular location for ILK. Reason: Correct core localization. |
| GO:0016020 membrane | HDA PMID:19946888 Defining the membrane proteome of NK cells. | KEEP AS NON CORE | Summary: membrane: a secondary/broad localization for ILK. Reason: Plausible but non-core (broad term or context-specific). |
| GO:0005829 cytosol | TAS Reactome:R-HSA-432897 | ACCEPT | Summary: cytosol: a core subcellular location for ILK. Reason: Correct core localization. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-446391 | ACCEPT | Summary: cytosol: a core subcellular location for ILK. Reason: Correct core localization. |
| GO:0019901 protein kinase binding | IPI PMID:19118217 EphA1 interacts with integrin-linked kinase and regulates ce... | KEEP AS NON CORE | Summary: Protein kinase binding (IPI): ILK scaffolds/associates with kinases at adhesions. Reason: Specific interaction supporting the scaffold role; non-core. |
| GO:0034446 substrate adhesion-dependent cell spreading | IMP PMID:19118217 EphA1 interacts with integrin-linked kinase and regulates ce... | ACCEPT | Summary: substrate adhesion-dependent cell spreading: a core biological process for ILK. Reason: Core process consistent with ILK's established function. |
| GO:0045893 positive regulation of DNA-templated transcription | IMP PMID:20018240 Type I collagen promotes epithelial-mesenchymal transition t... | KEEP AS NON CORE | Summary: positive regulation of DNA-templated transcription: process annotation for ILK. Reason: Valid but non-core / secondary. |
| GO:0090263 positive regulation of canonical Wnt signaling pathway | IMP PMID:20018240 Type I collagen promotes epithelial-mesenchymal transition t... | KEEP AS NON CORE | Summary: positive regulation of canonical Wnt signaling pathway: process annotation for ILK. Reason: Valid but non-core / secondary. |
| GO:0007160 cell-matrix adhesion | TAS PMID:8538749 Regulation of cell adhesion and anchorage-dependent growth b... | ACCEPT | Summary: cell-matrix adhesion: a core biological process for ILK. Reason: Core process consistent with ILK's established function. |
| GO:0007229 integrin-mediated signaling pathway | IDA PMID:9366252 Identification and characterization of a mouse protein kinas... | ACCEPT | Summary: integrin-mediated signaling pathway: a core biological process for ILK. Reason: Core process consistent with ILK's established function. |
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Download this section (compressed HTML)Q: Given ILK is a pseudokinase, are any residual phenotypes attributed to 'ILK kinase activity' in older literature explained entirely by its scaffolding of bona fide kinases?
Experiment: Rescue ILK-null cells with wild-type vs IPP-binding-deficient ILK and assess focal-adhesion assembly and spreading.
Hypothesis: ILK's adhesion functions are scaffold-dependent and kinase-activity-independent.
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