KIF5B encodes the ubiquitous kinesin-1 heavy chain, an ATP-dependent, plus-end-directed microtubule motor. Its core molecular role is to form kinesin-1 motor complexes that bind microtubules and move vesicles and organelles toward microtubule plus ends. KIF5B is recruited to many cargos through adaptors, including Arl8/SKIP-dependent lysosome positioning, lytic granule polarization in natural killer cells, mitochondrial transport, neuronal cargo transport, centrosome/nuclear positioning, and channel trafficking. Its lysosomal role is kinesin-dependent lysosome localization/positioning through adaptor-mediated cargo recruitment.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005871 kinesin complex | IBA GO_REF:0000033 | ACCEPT | Summary: KIF5B is a heavy-chain subunit of the kinesin-1 motor complex, which contains two KIF5B heavy chains and kinesin light chains. Reason: The cellular-component annotation reflects the canonical kinesin-1 complex architecture. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt Oligomer composed of two heavy chains and two light chains. |
| GO:0007411 axon guidance | IBA GO_REF:0000033 | MARK AS OVER ANNOTATED | Summary: This is a downstream neuronal phenotype or synaptic consequence inferred for kinesin-family cargo transport rather than a direct molecular or cellular core role of KIF5B. Reason: The direct KIF5B function is motor-driven cargo transport; annotating broad neuronal outcomes as KIF5B functions overstates the evidence. Proposed replacements: vesicle transport along microtubule |
| GO:0048489 synaptic vesicle transport | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: This is a plausible cargo- or cell-type-specific transport role for kinesin-1, but it is not the defining KIF5B function. Reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core role is plus-end-directed microtubule cargo transport. Supporting Evidence: PMID:28426968 distinct kinesins differentially regulate cargo velocity: kinesin-3 drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport |
| GO:0098971 anterograde dendritic transport of neurotransmitter receptor complex | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: This is a plausible cargo- or cell-type-specific transport role for kinesin-1, but it is not the defining KIF5B function. Reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core role is plus-end-directed microtubule cargo transport. Supporting Evidence: PMID:28426968 distinct kinesins differentially regulate cargo velocity: kinesin-3 drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport |
| GO:0008574 plus-end-directed microtubule motor activity | IBA GO_REF:0000033 | ACCEPT | Summary: This is the most informative existing molecular-function annotation for KIF5B. KIF5B is the kinesin-1 heavy chain and acts as a plus-end-directed microtubule motor. Reason: Retain as the core molecular function. Supporting Evidence: PMID:8606779 Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. file:human/KIF5B/KIF5B-uniprot.txt Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. |
| GO:0003777 microtubule motor activity | IEA GO_REF:0000120 | MODIFY | Summary: The annotation is correct but too broad for KIF5B, whose directional kinesin-1 activity is better captured by plus-end-directed microtubule motor activity. Reason: Replace the generic motor term with the specific plus-end-directed microtubule motor term already present in GOA. Proposed replacements: plus-end-directed microtubule motor activity Supporting Evidence: PMID:8606779 Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. file:human/KIF5B/KIF5B-uniprot.txt Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. |
| GO:0005524 ATP binding | IEA GO_REF:0000002 | MODIFY | Summary: The binding annotation is mechanistically true for kinesin-1 but less informative than the full ATP-dependent motor activity. Reason: Use the specific plus-end-directed microtubule motor activity rather than separate generic ATP-binding or microtubule-binding terms as the reviewed molecular function. Proposed replacements: plus-end-directed microtubule motor activity Supporting Evidence: PMID:1607388 the kinesin heavy chain showed both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules PMID:8606779 Here, we report the crystal structure of the human kinesin motor domain with bound ADP determined to 1.8-A resolution by X-ray crystallography. |
| GO:0005765 lysosomal membrane | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: KIF5B is recruited to the cytosolic face of lysosomal membranes through adaptor systems such as Arl8/SKIP to drive lysosome positioning. Reason: Keep as a supported PN-relevant cargo-specific localization, but do not treat lysosomal membrane association as the defining location of KIF5B activity across all cargos. Supporting Evidence: PMID:22172677 endogenous KIF5B, the heavy chain of kinesin-1, accumulated on lysosomes in cells overexpressing Arl8b and SKIP PMID:22172677 when KIF5B was knocked down by >85% by RNAi, lysosomes were more centrally clustered |
| GO:0005856 cytoskeleton | IEA GO_REF:0000044 | ACCEPT | Summary: KIF5B is a cytoskeleton-associated microtubule motor. The term is broad but consistent with the core motor mechanism. Reason: Retain as a core cellular context for KIF5B motor activity. Supporting Evidence: PMID:1607388 the kinesin heavy chain showed both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules PMID:8606779 Here, we report the crystal structure of the human kinesin motor domain with bound ADP determined to 1.8-A resolution by X-ray crystallography. |
| GO:0007018 microtubule-based movement | IEA GO_REF:0000002 | MODIFY | Summary: Microtubule-based movement is correct but broad. KIF5B specifically functions as a kinesin-1 cargo motor, so vesicle transport along microtubule is the better process term. Reason: Replace the broad process with the cargo-transport process already supported in GOA. Proposed replacements: vesicle transport along microtubule Supporting Evidence: PMID:8606779 Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. file:human/KIF5B/KIF5B-uniprot.txt Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. |
| GO:0008017 microtubule binding | IEA GO_REF:0000002 | MODIFY | Summary: The binding annotation is mechanistically true for kinesin-1 but less informative than the full ATP-dependent motor activity. Reason: Use the specific plus-end-directed microtubule motor activity rather than separate generic ATP-binding or microtubule-binding terms as the reviewed molecular function. Proposed replacements: plus-end-directed microtubule motor activity Supporting Evidence: PMID:1607388 the kinesin heavy chain showed both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules PMID:8606779 Here, we report the crystal structure of the human kinesin motor domain with bound ADP determined to 1.8-A resolution by X-ray crystallography. |
| GO:0032839 dendrite cytoplasm | IEA GO_REF:0000108 | KEEP AS NON CORE | Summary: This neuronal compartment annotation is consistent with kinesin cargo transport in neurons but is not a core localization for the ubiquitous KIF5B motor. Reason: Keep as a transferred, non-core neuronal localization context. |
| GO:0101004 cytolytic granule membrane | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: KIF5B participates in NK-cell lytic granule polarization and cytotoxicity through an Arl8b/SKIP/kinesin-1 cargo-transport pathway. Reason: Keep as a well-supported immune-cell cargo-specific role, secondary to the core kinesin motor function. Supporting Evidence: PMID:24088571 We also identified four unique peptides corresponding to KIF5B (the heavy chain of the plus-end motor protein kinesin-1 [KHC]) in the Arl8b pull down PMID:24088571 Silencing of KIF5B expression inhibited the polarization of both the MTOC and lytic granules to the immune synapse |
| GO:1904115 axon cytoplasm | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: This neuronal compartment annotation is consistent with kinesin cargo transport in neurons but is not a core localization for the ubiquitous KIF5B motor. Reason: Keep as a transferred, non-core neuronal localization context. |
| GO:0005515 protein binding | IPI PMID:15161933 Comprehensive proteomic analysis of interphase and mitotic 1... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:19164528 Nesprin 4 is an outer nuclear membrane protein that can indu... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:20562859 Network organization of the human autophagy system. | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:21915095 A kinesin-1 binding motif in vaccinia virus that is widespre... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:25855459 Repeated ER-endosome contacts promote endosome translocation... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:36931259 A central chaperone-like role for 14-3-3 proteins in human c... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0042802 identical protein binding | IPI PMID:17013387 Single-molecule observations of neck linker conformational c... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0042802 identical protein binding | IPI PMID:18004302 How kinesin waits between steps. | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005737 cytoplasm | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005739 mitochondrion | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: KIF5B can associate with diverse organelles or cellular regions through cargo adaptors, but this term is a cargo/context-specific location rather than the core motor role. Reason: Keep as non-core for a pleiotropic motor with many cargo associations. ORGANELLES check (2026-10-25), examined as a synthesis row. The Ensembl projection comes from mouse Kif5b (Q61768), whose only mitochondrion annotation is an MGI IDA from the mitocytosis study (PMID:34048705; QuickGO, checked 2026-10-25), so the projection is not circular and drops no NOT row. That cached copy is abstract-only and does not name Kif5b, so the assay is deferred to the curator. The organelle claim is consistent with the motor's curated role in mitochondrial distribution and its complex with the Miro/TRAK adaptors (RHOT1, RHOT2, TRAK1). KIF5B is a soluble motor with no transit peptide or TM segment that binds the mitochondrial surface through those adaptors, a recruited cargo association like the TRAF3/BRAF surface pools, so it stays at the root and GO:0005741 is not added. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. file:human/KIF5B/KIF5B-uniprot.txt Found in a complex with OGT, RHOT1, RHOT2 and TRAK1 (PubMed:24995978). |
| GO:0030139 endocytic vesicle | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: KIF5B can associate with diverse organelles or cellular regions through cargo adaptors, but this term is a cargo/context-specific location rather than the core motor role. Reason: Keep as non-core for a pleiotropic motor with many cargo associations. |
| GO:0032230 positive regulation of synaptic transmission, GABAergic | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: This is a downstream neuronal phenotype or synaptic consequence inferred for kinesin-family cargo transport rather than a direct molecular or cellular core role of KIF5B. Reason: The direct KIF5B function is motor-driven cargo transport; annotating broad neuronal outcomes as KIF5B functions overstates the evidence. Proposed replacements: vesicle transport along microtubule |
| GO:0035253 ciliary rootlet | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: KIF5B can associate with diverse organelles or cellular regions through cargo adaptors, but this term is a cargo/context-specific location rather than the core motor role. Reason: Keep as non-core for a pleiotropic motor with many cargo associations. |
| GO:0035617 stress granule disassembly | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: This is a plausible cargo- or cell-type-specific transport role for kinesin-1, but it is not the defining KIF5B function. Reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core role is plus-end-directed microtubule cargo transport. Supporting Evidence: PMID:28426968 distinct kinesins differentially regulate cargo velocity: kinesin-3 drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport |
| GO:0043005 neuron projection | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: This neuronal compartment annotation is consistent with kinesin cargo transport in neurons but is not a core localization for the ubiquitous KIF5B motor. Reason: Keep as a transferred, non-core neuronal localization context. |
| GO:0044877 protein-containing complex binding | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0045335 phagocytic vesicle | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: KIF5B can associate with diverse organelles or cellular regions through cargo adaptors, but this term is a cargo/context-specific location rather than the core motor role. Reason: Keep as non-core for a pleiotropic motor with many cargo associations. |
| GO:0098987 regulation of modification of synapse structure, modulating synaptic transmission | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: This is a downstream neuronal phenotype or synaptic consequence inferred for kinesin-family cargo transport rather than a direct molecular or cellular core role of KIF5B. Reason: The direct KIF5B function is motor-driven cargo transport; annotating broad neuronal outcomes as KIF5B functions overstates the evidence. Proposed replacements: vesicle transport along microtubule |
| GO:0099524 postsynaptic cytosol | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: This seeded annotation was reviewed as a non-core KIF5B context. Reason: KIF5B is a pleiotropic microtubule motor; this term is retained only as a secondary context. |
| GO:0005829 cytosol | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005765 lysosomal membrane | EXP PMID:22172677 Arl8 and SKIP act together to link lysosomes to kinesin-1. | KEEP AS NON CORE | Summary: KIF5B is recruited to the cytosolic face of lysosomal membranes through adaptor systems such as Arl8/SKIP to drive lysosome positioning. Reason: Keep as a supported PN-relevant cargo-specific localization, but do not treat lysosomal membrane association as the defining location of KIF5B activity across all cargos. Supporting Evidence: PMID:22172677 endogenous KIF5B, the heavy chain of kinesin-1, accumulated on lysosomes in cells overexpressing Arl8b and SKIP PMID:22172677 when KIF5B was knocked down by >85% by RNAi, lysosomes were more centrally clustered |
| GO:0005765 lysosomal membrane | EXP PMID:24088571 Arf-like GTPase Arl8b regulates lytic granule polarization a... | KEEP AS NON CORE | Summary: KIF5B is recruited to the cytosolic face of lysosomal membranes through adaptor systems such as Arl8/SKIP to drive lysosome positioning. Reason: Keep as a supported PN-relevant cargo-specific localization, but do not treat lysosomal membrane association as the defining location of KIF5B activity across all cargos. Supporting Evidence: PMID:22172677 endogenous KIF5B, the heavy chain of kinesin-1, accumulated on lysosomes in cells overexpressing Arl8b and SKIP PMID:22172677 when KIF5B was knocked down by >85% by RNAi, lysosomes were more centrally clustered |
| GO:0101004 cytolytic granule membrane | EXP PMID:24088571 Arf-like GTPase Arl8b regulates lytic granule polarization a... | KEEP AS NON CORE | Summary: KIF5B participates in NK-cell lytic granule polarization and cytotoxicity through an Arl8b/SKIP/kinesin-1 cargo-transport pathway. Reason: Keep as a well-supported immune-cell cargo-specific role, secondary to the core kinesin motor function. Supporting Evidence: PMID:24088571 We also identified four unique peptides corresponding to KIF5B (the heavy chain of the plus-end motor protein kinesin-1 [KHC]) in the Arl8b pull down PMID:24088571 Silencing of KIF5B expression inhibited the polarization of both the MTOC and lytic granules to the immune synapse |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9700181 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9712078 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9712079 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9712083 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9712084 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9712085 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9850958 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005515 protein binding | IPI PMID:28426968 MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- v... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0042267 natural killer cell mediated cytotoxicity | IMP PMID:24088571 Arf-like GTPase Arl8b regulates lytic granule polarization a... | KEEP AS NON CORE | Summary: KIF5B participates in NK-cell lytic granule polarization and cytotoxicity through an Arl8b/SKIP/kinesin-1 cargo-transport pathway. Reason: Keep as a well-supported immune-cell cargo-specific role, secondary to the core kinesin motor function. Supporting Evidence: PMID:24088571 We also identified four unique peptides corresponding to KIF5B (the heavy chain of the plus-end motor protein kinesin-1 [KHC]) in the Arl8b pull down PMID:24088571 Silencing of KIF5B expression inhibited the polarization of both the MTOC and lytic granules to the immune synapse |
| GO:0032418 lysosome localization | IMP PMID:22172677 Arl8 and SKIP act together to link lysosomes to kinesin-1. | KEEP AS NON CORE | Summary: KIF5B has direct evidence for lysosome positioning/localization through Arl8/SKIP-dependent recruitment of kinesin-1 to lysosomes. Reason: Keep as the supported Proteostasis Network-relevant cargo-specific process. This does not justify propagating the broader PN lysosomal-tubulation context to lysosome organization. Supporting Evidence: PMID:22172677 endogenous KIF5B, the heavy chain of kinesin-1, accumulated on lysosomes in cells overexpressing Arl8b and SKIP PMID:22172677 when KIF5B was knocked down by >85% by RNAi, lysosomes were more centrally clustered |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9700179 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0099641 anterograde axonal protein transport | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: This is a plausible cargo- or cell-type-specific transport role for kinesin-1, but it is not the defining KIF5B function. Reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core role is plus-end-directed microtubule cargo transport. Supporting Evidence: PMID:28426968 distinct kinesins differentially regulate cargo velocity: kinesin-3 drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport |
| GO:0045296 cadherin binding | HDA PMID:25468996 E-cadherin interactome complexity and robustness resolved by... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0003777 microtubule motor activity | IDA PMID:28426968 MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- v... | MODIFY | Summary: The annotation is correct but too broad for KIF5B, whose directional kinesin-1 activity is better captured by plus-end-directed microtubule motor activity. Reason: Replace the generic motor term with the specific plus-end-directed microtubule motor term already present in GOA. Proposed replacements: plus-end-directed microtubule motor activity Supporting Evidence: PMID:8606779 Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. file:human/KIF5B/KIF5B-uniprot.txt Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. |
| GO:0008017 microtubule binding | IDA PMID:28426968 MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- v... | MODIFY | Summary: The binding annotation is mechanistically true for kinesin-1 but less informative than the full ATP-dependent motor activity. Reason: Use the specific plus-end-directed microtubule motor activity rather than separate generic ATP-binding or microtubule-binding terms as the reviewed molecular function. Proposed replacements: plus-end-directed microtubule motor activity Supporting Evidence: PMID:1607388 the kinesin heavy chain showed both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules PMID:8606779 Here, we report the crystal structure of the human kinesin motor domain with bound ADP determined to 1.8-A resolution by X-ray crystallography. |
| GO:0047496 vesicle transport along microtubule | IDA PMID:28426968 MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- v... | ACCEPT | Summary: This process captures the core cargo-transport role of KIF5B as a kinesin-1 motor moving vesicles and organelles along microtubules. Reason: Retain as a core biological-process abstraction for KIF5B motor function. Supporting Evidence: PMID:24088571 Kinesin-1 regulates long-range anterograde intracellular transport of vesicles, including lysosomes |
| GO:1990048 anterograde neuronal dense core vesicle transport | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: This is a plausible cargo- or cell-type-specific transport role for kinesin-1, but it is not the defining KIF5B function. Reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core role is plus-end-directed microtubule cargo transport. Supporting Evidence: PMID:28426968 distinct kinesins differentially regulate cargo velocity: kinesin-3 drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport |
| GO:1990049 retrograde neuronal dense core vesicle transport | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: This is a plausible cargo- or cell-type-specific transport role for kinesin-1, but it is not the defining KIF5B function. Reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core role is plus-end-directed microtubule cargo transport. Supporting Evidence: PMID:28426968 distinct kinesins differentially regulate cargo velocity: kinesin-3 drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport |
| GO:0051642 centrosome localization | IMP PMID:20386726 Bicaudal D2, dynein, and kinesin-1 associate with nuclear po... | KEEP AS NON CORE | Summary: KIF5B contributes to centrosome and nuclear positioning during mitotic entry through antagonism with dynein-dependent forces. Reason: Keep as a supported cell-cycle positioning role, but not as the core function of this pleiotropic motor. Supporting Evidence: PMID:20386726 we show that while dynein pulls centrosomes and the nucleus together during mitotic entry, kinesin-1 pushes them apart |
| GO:0016020 membrane | HDA PMID:19946888 Defining the membrane proteome of NK cells. | KEEP AS NON CORE | Summary: Membrane association is documented for this protein, so the generic membrane term is true, but it is not the protein's core location. Reason: UniProt: cytolytic granule membrane (ECO:0000269, PubMed:24088571) and lysosome membrane, peripheral; EXP leads. Membrane association is documented, including in NK-cell granules. (Manual re-review for OMICS_EVIDENCE, 2026-10-10; previous action: MARK_AS_OVER_ANNOTATED.) Proposed replacements: lysosomal membrane cytolytic granule membrane |
| GO:0042391 regulation of membrane potential | IDA PMID:19675065 Kif5b is an essential forward trafficking motor for the Kv1.... | MODIFY | Summary: These annotations describe downstream electrophysiological consequences of KIF5B-dependent Kv1.5 channel trafficking rather than the direct KIF5B process. Reason: Replace membrane-potential/potassium-transport regulation with the more direct protein-localization-to-plasma-membrane process supported by the original study. Proposed replacements: positive regulation of protein localization to plasma membrane Supporting Evidence: PMID:19675065 Thus, Kif5b is essential to anterograde trafficking of a cardiac voltage-gated potassium channel. |
| GO:0043268 positive regulation of potassium ion transport | IDA PMID:19675065 Kif5b is an essential forward trafficking motor for the Kv1.... | MODIFY | Summary: These annotations describe downstream electrophysiological consequences of KIF5B-dependent Kv1.5 channel trafficking rather than the direct KIF5B process. Reason: Replace membrane-potential/potassium-transport regulation with the more direct protein-localization-to-plasma-membrane process supported by the original study. Proposed replacements: positive regulation of protein localization to plasma membrane Supporting Evidence: PMID:19675065 Thus, Kif5b is essential to anterograde trafficking of a cardiac voltage-gated potassium channel. |
| GO:1903078 positive regulation of protein localization to plasma membrane | IDA PMID:19675065 Kif5b is an essential forward trafficking motor for the Kv1.... | KEEP AS NON CORE | Summary: This captures the direct mechanism in the Kv1.5 study: KIF5B promotes forward trafficking and surface localization of the channel. Reason: Keep as a supported cargo-specific channel-trafficking role, secondary to the core microtubule motor function. Supporting Evidence: PMID:19675065 Thus, Kif5b is essential to anterograde trafficking of a cardiac voltage-gated potassium channel. |
| GO:0031982 vesicle | IDA PMID:20682791 A protein interaction network for Ecm29 links the 26 S prote... | KEEP AS NON CORE | Summary: KIF5B can associate with diverse organelles or cellular regions through cargo adaptors, but this term is a cargo/context-specific location rather than the core motor role. Reason: Keep as non-core for a pleiotropic motor with many cargo associations. |
| GO:0035617 stress granule disassembly | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: This is a plausible cargo- or cell-type-specific transport role for kinesin-1, but it is not the defining KIF5B function. Reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core role is plus-end-directed microtubule cargo transport. Supporting Evidence: PMID:28426968 distinct kinesins differentially regulate cargo velocity: kinesin-3 drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport |
| GO:0003777 microtubule motor activity | ISS GO_REF:0000024 | MODIFY | Summary: The annotation is correct but too broad for KIF5B, whose directional kinesin-1 activity is better captured by plus-end-directed microtubule motor activity. Reason: Replace the generic motor term with the specific plus-end-directed microtubule motor term already present in GOA. Proposed replacements: plus-end-directed microtubule motor activity Supporting Evidence: PMID:8606779 Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. file:human/KIF5B/KIF5B-uniprot.txt Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. |
| GO:0008017 microtubule binding | ISS GO_REF:0000024 | MODIFY | Summary: The binding annotation is mechanistically true for kinesin-1 but less informative than the full ATP-dependent motor activity. Reason: Use the specific plus-end-directed microtubule motor activity rather than separate generic ATP-binding or microtubule-binding terms as the reviewed molecular function. Proposed replacements: plus-end-directed microtubule motor activity Supporting Evidence: PMID:1607388 the kinesin heavy chain showed both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules PMID:8606779 Here, we report the crystal structure of the human kinesin motor domain with bound ADP determined to 1.8-A resolution by X-ray crystallography. |
| GO:0047496 vesicle transport along microtubule | ISS GO_REF:0000024 | ACCEPT | Summary: This process captures the core cargo-transport role of KIF5B as a kinesin-1 motor moving vesicles and organelles along microtubules. Reason: Retain as a core biological-process abstraction for KIF5B motor function. Supporting Evidence: PMID:24088571 Kinesin-1 regulates long-range anterograde intracellular transport of vesicles, including lysosomes |
| GO:0048471 perinuclear region of cytoplasm | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: KIF5B can associate with diverse organelles or cellular regions through cargo adaptors, but this term is a cargo/context-specific location rather than the core motor role. Reason: Keep as non-core for a pleiotropic motor with many cargo associations. |
| GO:0003777 microtubule motor activity | TAS PMID:8606779 Crystal structure of the kinesin motor domain reveals a stru... | MODIFY | Summary: The annotation is correct but too broad for KIF5B, whose directional kinesin-1 activity is better captured by plus-end-directed microtubule motor activity. Reason: Replace the generic motor term with the specific plus-end-directed microtubule motor term already present in GOA. Proposed replacements: plus-end-directed microtubule motor activity Supporting Evidence: PMID:8606779 Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. file:human/KIF5B/KIF5B-uniprot.txt Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. |
| GO:0005871 kinesin complex | TAS PMID:1607388 Cloning and expression of a human kinesin heavy chain gene: ... | ACCEPT | Summary: KIF5B is a heavy-chain subunit of the kinesin-1 motor complex, which contains two KIF5B heavy chains and kinesin light chains. Reason: The cellular-component annotation reflects the canonical kinesin-1 complex architecture. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt Oligomer composed of two heavy chains and two light chains. |
| GO:0007018 microtubule-based movement | TAS PMID:7514426 Cloning and localization of a conventional kinesin motor exp... | MODIFY | Summary: Microtubule-based movement is correct but broad. KIF5B specifically functions as a kinesin-1 cargo motor, so vesicle transport along microtubule is the better process term. Reason: Replace the broad process with the cargo-transport process already supported in GOA. Proposed replacements: vesicle transport along microtubule Supporting Evidence: PMID:8606779 Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. file:human/KIF5B/KIF5B-uniprot.txt Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. |
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Download this section (compressed HTML)Q: Does direct starvation-recovery or autophagic lysosome reformation evidence support annotating KIF5B to a lysosome-organization/ALR term, or should KIF5B remain annotated to lysosome localization only?
Suggested experts: Sean Munro, Cleo T. Schiefermeier
Q: Should GO curation distinguish kinesin-1 heavy-chain core motor activity from cargo-specific downstream outcomes such as NK cytotoxicity, channel trafficking, and neuronal synaptic phenotypes?
Suggested experts: GO curator, kinesin-1 domain expert
Experiment: Measure KIF5B recruitment to autolysosomes and lysosomal tubules during starvation and nutrient refeeding, using endogenous tagging plus KIF5B depletion/rescue.
Hypothesis: KIF5B contributes to lysosome positioning during recovery but requires additional evidence before annotation to autophagic lysosome reformation or lysosome organization.
Type: live-cell imaging/genetic rescue
Experiment: Compare wild-type KIF5B and motor-dead or cargo-adaptor-binding mutants for lysosome localization, lytic granule polarization, and generic vesicle transport in matched cell systems.
Hypothesis: Cargo-specific KIF5B phenotypes depend on the same core plus-end-directed motor activity but separable adaptor interactions.
Type: structure-function perturbation
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