KIF5B encodes the ubiquitous kinesin-1 heavy chain, an ATP-dependent, plus-end-directed microtubule motor. Its core molecular role is to form kinesin-1 motor complexes that bind microtubules and move vesicles and organelles toward microtubule plus ends. KIF5B is recruited to many cargos through adaptors, including Arl8/SKIP-dependent lysosome positioning, lytic granule polarization in natural killer cells, mitochondrial transport, neuronal cargo transport, centrosome/nuclear positioning, and channel trafficking. Its lysosomal role is kinesin-dependent lysosome localization/positioning through adaptor-mediated cargo recruitment.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005871 kinesin complex | IBA GO_REF:0000033 | ACCEPT | Summary: KIF5B is a heavy-chain subunit of the kinesin-1 motor complex, which contains two KIF5B heavy chains and kinesin light chains. Reason: The cellular-component annotation reflects the canonical kinesin-1 complex architecture. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt Oligomer composed of two heavy chains and two light chains. |
| GO:0007411 axon guidance | IBA GO_REF:0000033 | MARK AS OVER ANNOTATED | Summary: This is a downstream neuronal phenotype or synaptic consequence inferred for kinesin-family cargo transport rather than a direct molecular or cellular core role of KIF5B. Reason: The direct KIF5B function is motor-driven cargo transport; annotating broad neuronal outcomes as KIF5B functions overstates the evidence. Proposed replacements: vesicle transport along microtubule |
| GO:0048489 synaptic vesicle transport | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: This is a plausible cargo- or cell-type-specific transport role for kinesin-1, but it is not the defining KIF5B function. Reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core role is plus-end-directed microtubule cargo transport. Supporting Evidence: PMID:28426968 distinct kinesins differentially regulate cargo velocity: kinesin-3 drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport |
| GO:0098971 anterograde dendritic transport of neurotransmitter receptor complex | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: This is a plausible cargo- or cell-type-specific transport role for kinesin-1, but it is not the defining KIF5B function. Reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core role is plus-end-directed microtubule cargo transport. Supporting Evidence: PMID:28426968 distinct kinesins differentially regulate cargo velocity: kinesin-3 drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport |
| GO:0008574 plus-end-directed microtubule motor activity | IBA GO_REF:0000033 | ACCEPT | Summary: This is the most informative existing molecular-function annotation for KIF5B. KIF5B is the kinesin-1 heavy chain and acts as a plus-end-directed microtubule motor. Reason: Retain as the core molecular function. Supporting Evidence: PMID:8606779 Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. file:human/KIF5B/KIF5B-uniprot.txt Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. |
| GO:0003777 microtubule motor activity | IEA GO_REF:0000120 | MODIFY | Summary: The annotation is correct but too broad for KIF5B, whose directional kinesin-1 activity is better captured by plus-end-directed microtubule motor activity. Reason: Replace the generic motor term with the specific plus-end-directed microtubule motor term already present in GOA. Proposed replacements: plus-end-directed microtubule motor activity Supporting Evidence: PMID:8606779 Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. file:human/KIF5B/KIF5B-uniprot.txt Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. |
| GO:0005524 ATP binding | IEA GO_REF:0000002 | MODIFY | Summary: The binding annotation is mechanistically true for kinesin-1 but less informative than the full ATP-dependent motor activity. Reason: Use the specific plus-end-directed microtubule motor activity rather than separate generic ATP-binding or microtubule-binding terms as the reviewed molecular function. Proposed replacements: plus-end-directed microtubule motor activity Supporting Evidence: PMID:1607388 the kinesin heavy chain showed both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules PMID:8606779 Here, we report the crystal structure of the human kinesin motor domain with bound ADP determined to 1.8-A resolution by X-ray crystallography. |
| GO:0005765 lysosomal membrane | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: KIF5B is recruited to the cytosolic face of lysosomal membranes through adaptor systems such as Arl8/SKIP to drive lysosome positioning. Reason: Keep as a supported PN-relevant cargo-specific localization, but do not treat lysosomal membrane association as the defining location of KIF5B activity across all cargos. Supporting Evidence: PMID:22172677 endogenous KIF5B, the heavy chain of kinesin-1, accumulated on lysosomes in cells overexpressing Arl8b and SKIP PMID:22172677 when KIF5B was knocked down by >85% by RNAi, lysosomes were more centrally clustered |
| GO:0005856 cytoskeleton | IEA GO_REF:0000044 | ACCEPT | Summary: KIF5B is a cytoskeleton-associated microtubule motor. The term is broad but consistent with the core motor mechanism. Reason: Retain as a core cellular context for KIF5B motor activity. Supporting Evidence: PMID:1607388 the kinesin heavy chain showed both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules PMID:8606779 Here, we report the crystal structure of the human kinesin motor domain with bound ADP determined to 1.8-A resolution by X-ray crystallography. |
| GO:0007018 microtubule-based movement | IEA GO_REF:0000002 | MODIFY | Summary: Microtubule-based movement is correct but broad. KIF5B specifically functions as a kinesin-1 cargo motor, so vesicle transport along microtubule is the better process term. Reason: Replace the broad process with the cargo-transport process already supported in GOA. Proposed replacements: vesicle transport along microtubule Supporting Evidence: PMID:8606779 Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. file:human/KIF5B/KIF5B-uniprot.txt Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. |
| GO:0008017 microtubule binding | IEA GO_REF:0000002 | MODIFY | Summary: The binding annotation is mechanistically true for kinesin-1 but less informative than the full ATP-dependent motor activity. Reason: Use the specific plus-end-directed microtubule motor activity rather than separate generic ATP-binding or microtubule-binding terms as the reviewed molecular function. Proposed replacements: plus-end-directed microtubule motor activity Supporting Evidence: PMID:1607388 the kinesin heavy chain showed both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules PMID:8606779 Here, we report the crystal structure of the human kinesin motor domain with bound ADP determined to 1.8-A resolution by X-ray crystallography. |
| GO:0032839 dendrite cytoplasm | IEA GO_REF:0000108 | KEEP AS NON CORE | Summary: This neuronal compartment annotation is consistent with kinesin cargo transport in neurons but is not a core localization for the ubiquitous KIF5B motor. Reason: Keep as a transferred, non-core neuronal localization context. |
| GO:0101004 cytolytic granule membrane | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: KIF5B participates in NK-cell lytic granule polarization and cytotoxicity through an Arl8b/SKIP/kinesin-1 cargo-transport pathway. Reason: Keep as a well-supported immune-cell cargo-specific role, secondary to the core kinesin motor function. Supporting Evidence: PMID:24088571 We also identified four unique peptides corresponding to KIF5B (the heavy chain of the plus-end motor protein kinesin-1 [KHC]) in the Arl8b pull down PMID:24088571 Silencing of KIF5B expression inhibited the polarization of both the MTOC and lytic granules to the immune synapse |
| GO:1904115 axon cytoplasm | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: This neuronal compartment annotation is consistent with kinesin cargo transport in neurons but is not a core localization for the ubiquitous KIF5B motor. Reason: Keep as a transferred, non-core neuronal localization context. |
| GO:0005515 protein binding | IPI PMID:15161933 Comprehensive proteomic analysis of interphase and mitotic 1... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:19164528 Nesprin 4 is an outer nuclear membrane protein that can indu... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:20562859 Network organization of the human autophagy system. | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:21915095 A kinesin-1 binding motif in vaccinia virus that is widespre... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:25855459 Repeated ER-endosome contacts promote endosome translocation... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005515 protein binding | IPI PMID:36931259 A central chaperone-like role for 14-3-3 proteins in human c... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0042802 identical protein binding | IPI PMID:17013387 Single-molecule observations of neck linker conformational c... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0042802 identical protein binding | IPI PMID:18004302 How kinesin waits between steps. | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0005737 cytoplasm | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005739 mitochondrion | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: KIF5B can associate with diverse organelles or cellular regions through cargo adaptors, but this term is a cargo/context-specific location rather than the core motor role. Reason: Keep as non-core for a pleiotropic motor with many cargo associations. |
| GO:0030139 endocytic vesicle | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: KIF5B can associate with diverse organelles or cellular regions through cargo adaptors, but this term is a cargo/context-specific location rather than the core motor role. Reason: Keep as non-core for a pleiotropic motor with many cargo associations. |
| GO:0032230 positive regulation of synaptic transmission, GABAergic | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: This is a downstream neuronal phenotype or synaptic consequence inferred for kinesin-family cargo transport rather than a direct molecular or cellular core role of KIF5B. Reason: The direct KIF5B function is motor-driven cargo transport; annotating broad neuronal outcomes as KIF5B functions overstates the evidence. Proposed replacements: vesicle transport along microtubule |
| GO:0035253 ciliary rootlet | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: KIF5B can associate with diverse organelles or cellular regions through cargo adaptors, but this term is a cargo/context-specific location rather than the core motor role. Reason: Keep as non-core for a pleiotropic motor with many cargo associations. |
| GO:0035617 stress granule disassembly | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: This is a plausible cargo- or cell-type-specific transport role for kinesin-1, but it is not the defining KIF5B function. Reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core role is plus-end-directed microtubule cargo transport. Supporting Evidence: PMID:28426968 distinct kinesins differentially regulate cargo velocity: kinesin-3 drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport |
| GO:0043005 neuron projection | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: This neuronal compartment annotation is consistent with kinesin cargo transport in neurons but is not a core localization for the ubiquitous KIF5B motor. Reason: Keep as a transferred, non-core neuronal localization context. |
| GO:0044877 protein-containing complex binding | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0045335 phagocytic vesicle | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: KIF5B can associate with diverse organelles or cellular regions through cargo adaptors, but this term is a cargo/context-specific location rather than the core motor role. Reason: Keep as non-core for a pleiotropic motor with many cargo associations. |
| GO:0098987 regulation of modification of synapse structure, modulating synaptic transmission | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: This is a downstream neuronal phenotype or synaptic consequence inferred for kinesin-family cargo transport rather than a direct molecular or cellular core role of KIF5B. Reason: The direct KIF5B function is motor-driven cargo transport; annotating broad neuronal outcomes as KIF5B functions overstates the evidence. Proposed replacements: vesicle transport along microtubule |
| GO:0099524 postsynaptic cytosol | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: This seeded annotation was reviewed as a non-core KIF5B context. Reason: KIF5B is a pleiotropic microtubule motor; this term is retained only as a secondary context. |
| GO:0005829 cytosol | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005765 lysosomal membrane | EXP PMID:22172677 Arl8 and SKIP act together to link lysosomes to kinesin-1. | KEEP AS NON CORE | Summary: KIF5B is recruited to the cytosolic face of lysosomal membranes through adaptor systems such as Arl8/SKIP to drive lysosome positioning. Reason: Keep as a supported PN-relevant cargo-specific localization, but do not treat lysosomal membrane association as the defining location of KIF5B activity across all cargos. Supporting Evidence: PMID:22172677 endogenous KIF5B, the heavy chain of kinesin-1, accumulated on lysosomes in cells overexpressing Arl8b and SKIP PMID:22172677 when KIF5B was knocked down by >85% by RNAi, lysosomes were more centrally clustered |
| GO:0005765 lysosomal membrane | EXP PMID:24088571 Arf-like GTPase Arl8b regulates lytic granule polarization a... | KEEP AS NON CORE | Summary: KIF5B is recruited to the cytosolic face of lysosomal membranes through adaptor systems such as Arl8/SKIP to drive lysosome positioning. Reason: Keep as a supported PN-relevant cargo-specific localization, but do not treat lysosomal membrane association as the defining location of KIF5B activity across all cargos. Supporting Evidence: PMID:22172677 endogenous KIF5B, the heavy chain of kinesin-1, accumulated on lysosomes in cells overexpressing Arl8b and SKIP PMID:22172677 when KIF5B was knocked down by >85% by RNAi, lysosomes were more centrally clustered |
| GO:0101004 cytolytic granule membrane | EXP PMID:24088571 Arf-like GTPase Arl8b regulates lytic granule polarization a... | KEEP AS NON CORE | Summary: KIF5B participates in NK-cell lytic granule polarization and cytotoxicity through an Arl8b/SKIP/kinesin-1 cargo-transport pathway. Reason: Keep as a well-supported immune-cell cargo-specific role, secondary to the core kinesin motor function. Supporting Evidence: PMID:24088571 We also identified four unique peptides corresponding to KIF5B (the heavy chain of the plus-end motor protein kinesin-1 [KHC]) in the Arl8b pull down PMID:24088571 Silencing of KIF5B expression inhibited the polarization of both the MTOC and lytic granules to the immune synapse |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9700181 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9712078 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9712079 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9712083 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9712084 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9712085 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9850958 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0005515 protein binding | IPI PMID:28426968 MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- v... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0042267 natural killer cell mediated cytotoxicity | IMP PMID:24088571 Arf-like GTPase Arl8b regulates lytic granule polarization a... | KEEP AS NON CORE | Summary: KIF5B participates in NK-cell lytic granule polarization and cytotoxicity through an Arl8b/SKIP/kinesin-1 cargo-transport pathway. Reason: Keep as a well-supported immune-cell cargo-specific role, secondary to the core kinesin motor function. Supporting Evidence: PMID:24088571 We also identified four unique peptides corresponding to KIF5B (the heavy chain of the plus-end motor protein kinesin-1 [KHC]) in the Arl8b pull down PMID:24088571 Silencing of KIF5B expression inhibited the polarization of both the MTOC and lytic granules to the immune synapse |
| GO:0032418 lysosome localization | IMP PMID:22172677 Arl8 and SKIP act together to link lysosomes to kinesin-1. | KEEP AS NON CORE | Summary: KIF5B has direct evidence for lysosome positioning/localization through Arl8/SKIP-dependent recruitment of kinesin-1 to lysosomes. Reason: Keep as the supported Proteostasis Network-relevant cargo-specific process. This does not justify propagating the broader PN lysosomal-tubulation context to lysosome organization. Supporting Evidence: PMID:22172677 endogenous KIF5B, the heavy chain of kinesin-1, accumulated on lysosomes in cells overexpressing Arl8b and SKIP PMID:22172677 when KIF5B was knocked down by >85% by RNAi, lysosomes were more centrally clustered |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9700179 | KEEP AS NON CORE | Summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization does not describe the specific motor function. Reason: Keep as a correct broad location, not as a core functional annotation. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton |
| GO:0099641 anterograde axonal protein transport | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: This is a plausible cargo- or cell-type-specific transport role for kinesin-1, but it is not the defining KIF5B function. Reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core role is plus-end-directed microtubule cargo transport. Supporting Evidence: PMID:28426968 distinct kinesins differentially regulate cargo velocity: kinesin-3 drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport |
| GO:0045296 cadherin binding | HDA PMID:25468996 E-cadherin interactome complexity and robustness resolved by... | MARK AS OVER ANNOTATED | Summary: The interaction may be experimentally observed, but the GO term is generic and does not convey KIF5B biology. Reason: Do not use generic binding terms as reviewed KIF5B function; the informative molecular function is plus-end-directed microtubule motor activity and cargo transport. Proposed replacements: plus-end-directed microtubule motor activity |
| GO:0003777 microtubule motor activity | IDA PMID:28426968 MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- v... | MODIFY | Summary: The annotation is correct but too broad for KIF5B, whose directional kinesin-1 activity is better captured by plus-end-directed microtubule motor activity. Reason: Replace the generic motor term with the specific plus-end-directed microtubule motor term already present in GOA. Proposed replacements: plus-end-directed microtubule motor activity Supporting Evidence: PMID:8606779 Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. file:human/KIF5B/KIF5B-uniprot.txt Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. |
| GO:0008017 microtubule binding | IDA PMID:28426968 MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- v... | MODIFY | Summary: The binding annotation is mechanistically true for kinesin-1 but less informative than the full ATP-dependent motor activity. Reason: Use the specific plus-end-directed microtubule motor activity rather than separate generic ATP-binding or microtubule-binding terms as the reviewed molecular function. Proposed replacements: plus-end-directed microtubule motor activity Supporting Evidence: PMID:1607388 the kinesin heavy chain showed both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules PMID:8606779 Here, we report the crystal structure of the human kinesin motor domain with bound ADP determined to 1.8-A resolution by X-ray crystallography. |
| GO:0047496 vesicle transport along microtubule | IDA PMID:28426968 MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- v... | ACCEPT | Summary: This process captures the core cargo-transport role of KIF5B as a kinesin-1 motor moving vesicles and organelles along microtubules. Reason: Retain as a core biological-process abstraction for KIF5B motor function. Supporting Evidence: PMID:24088571 Kinesin-1 regulates long-range anterograde intracellular transport of vesicles, including lysosomes |
| GO:1990048 anterograde neuronal dense core vesicle transport | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: This is a plausible cargo- or cell-type-specific transport role for kinesin-1, but it is not the defining KIF5B function. Reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core role is plus-end-directed microtubule cargo transport. Supporting Evidence: PMID:28426968 distinct kinesins differentially regulate cargo velocity: kinesin-3 drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport |
| GO:1990049 retrograde neuronal dense core vesicle transport | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: This is a plausible cargo- or cell-type-specific transport role for kinesin-1, but it is not the defining KIF5B function. Reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core role is plus-end-directed microtubule cargo transport. Supporting Evidence: PMID:28426968 distinct kinesins differentially regulate cargo velocity: kinesin-3 drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport |
| GO:0051642 centrosome localization | IMP PMID:20386726 Bicaudal D2, dynein, and kinesin-1 associate with nuclear po... | KEEP AS NON CORE | Summary: KIF5B contributes to centrosome and nuclear positioning during mitotic entry through antagonism with dynein-dependent forces. Reason: Keep as a supported cell-cycle positioning role, but not as the core function of this pleiotropic motor. Supporting Evidence: PMID:20386726 we show that while dynein pulls centrosomes and the nucleus together during mitotic entry, kinesin-1 pushes them apart |
| GO:0016020 membrane | HDA PMID:19946888 Defining the membrane proteome of NK cells. | MARK AS OVER ANNOTATED | Summary: Broad membrane is a high-throughput location that loses the important biology. KIF5B is a peripheral/adaptor-recruited motor on specific cargos such as lysosomes and lytic granules, not a generic membrane protein. Reason: Retain the more specific cargo-membrane annotations where supported and avoid the uninformative broad membrane term. Proposed replacements: lysosomal membrane cytolytic granule membrane |
| GO:0042391 regulation of membrane potential | IDA PMID:19675065 Kif5b is an essential forward trafficking motor for the Kv1.... | MODIFY | Summary: These annotations describe downstream electrophysiological consequences of KIF5B-dependent Kv1.5 channel trafficking rather than the direct KIF5B process. Reason: Replace membrane-potential/potassium-transport regulation with the more direct protein-localization-to-plasma-membrane process supported by the original study. Proposed replacements: positive regulation of protein localization to plasma membrane Supporting Evidence: PMID:19675065 Thus, Kif5b is essential to anterograde trafficking of a cardiac voltage-gated potassium channel. |
| GO:0043268 positive regulation of potassium ion transport | IDA PMID:19675065 Kif5b is an essential forward trafficking motor for the Kv1.... | MODIFY | Summary: These annotations describe downstream electrophysiological consequences of KIF5B-dependent Kv1.5 channel trafficking rather than the direct KIF5B process. Reason: Replace membrane-potential/potassium-transport regulation with the more direct protein-localization-to-plasma-membrane process supported by the original study. Proposed replacements: positive regulation of protein localization to plasma membrane Supporting Evidence: PMID:19675065 Thus, Kif5b is essential to anterograde trafficking of a cardiac voltage-gated potassium channel. |
| GO:1903078 positive regulation of protein localization to plasma membrane | IDA PMID:19675065 Kif5b is an essential forward trafficking motor for the Kv1.... | KEEP AS NON CORE | Summary: This captures the direct mechanism in the Kv1.5 study: KIF5B promotes forward trafficking and surface localization of the channel. Reason: Keep as a supported cargo-specific channel-trafficking role, secondary to the core microtubule motor function. Supporting Evidence: PMID:19675065 Thus, Kif5b is essential to anterograde trafficking of a cardiac voltage-gated potassium channel. |
| GO:0031982 vesicle | IDA PMID:20682791 A protein interaction network for Ecm29 links the 26 S prote... | KEEP AS NON CORE | Summary: KIF5B can associate with diverse organelles or cellular regions through cargo adaptors, but this term is a cargo/context-specific location rather than the core motor role. Reason: Keep as non-core for a pleiotropic motor with many cargo associations. |
| GO:0035617 stress granule disassembly | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: This is a plausible cargo- or cell-type-specific transport role for kinesin-1, but it is not the defining KIF5B function. Reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core role is plus-end-directed microtubule cargo transport. Supporting Evidence: PMID:28426968 distinct kinesins differentially regulate cargo velocity: kinesin-3 drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport |
| GO:0003777 microtubule motor activity | ISS GO_REF:0000024 | MODIFY | Summary: The annotation is correct but too broad for KIF5B, whose directional kinesin-1 activity is better captured by plus-end-directed microtubule motor activity. Reason: Replace the generic motor term with the specific plus-end-directed microtubule motor term already present in GOA. Proposed replacements: plus-end-directed microtubule motor activity Supporting Evidence: PMID:8606779 Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. file:human/KIF5B/KIF5B-uniprot.txt Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. |
| GO:0008017 microtubule binding | ISS GO_REF:0000024 | MODIFY | Summary: The binding annotation is mechanistically true for kinesin-1 but less informative than the full ATP-dependent motor activity. Reason: Use the specific plus-end-directed microtubule motor activity rather than separate generic ATP-binding or microtubule-binding terms as the reviewed molecular function. Proposed replacements: plus-end-directed microtubule motor activity Supporting Evidence: PMID:1607388 the kinesin heavy chain showed both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules PMID:8606779 Here, we report the crystal structure of the human kinesin motor domain with bound ADP determined to 1.8-A resolution by X-ray crystallography. |
| GO:0047496 vesicle transport along microtubule | ISS GO_REF:0000024 | ACCEPT | Summary: This process captures the core cargo-transport role of KIF5B as a kinesin-1 motor moving vesicles and organelles along microtubules. Reason: Retain as a core biological-process abstraction for KIF5B motor function. Supporting Evidence: PMID:24088571 Kinesin-1 regulates long-range anterograde intracellular transport of vesicles, including lysosomes |
| GO:0048471 perinuclear region of cytoplasm | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: KIF5B can associate with diverse organelles or cellular regions through cargo adaptors, but this term is a cargo/context-specific location rather than the core motor role. Reason: Keep as non-core for a pleiotropic motor with many cargo associations. |
| GO:0003777 microtubule motor activity | TAS PMID:8606779 Crystal structure of the kinesin motor domain reveals a stru... | MODIFY | Summary: The annotation is correct but too broad for KIF5B, whose directional kinesin-1 activity is better captured by plus-end-directed microtubule motor activity. Reason: Replace the generic motor term with the specific plus-end-directed microtubule motor term already present in GOA. Proposed replacements: plus-end-directed microtubule motor activity Supporting Evidence: PMID:8606779 Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. file:human/KIF5B/KIF5B-uniprot.txt Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. |
| GO:0005871 kinesin complex | TAS PMID:1607388 Cloning and expression of a human kinesin heavy chain gene: ... | ACCEPT | Summary: KIF5B is a heavy-chain subunit of the kinesin-1 motor complex, which contains two KIF5B heavy chains and kinesin light chains. Reason: The cellular-component annotation reflects the canonical kinesin-1 complex architecture. Supporting Evidence: file:human/KIF5B/KIF5B-uniprot.txt Oligomer composed of two heavy chains and two light chains. |
| GO:0007018 microtubule-based movement | TAS PMID:7514426 Cloning and localization of a conventional kinesin motor exp... | MODIFY | Summary: Microtubule-based movement is correct but broad. KIF5B specifically functions as a kinesin-1 cargo motor, so vesicle transport along microtubule is the better process term. Reason: Replace the broad process with the cargo-transport process already supported in GOA. Proposed replacements: vesicle transport along microtubule Supporting Evidence: PMID:8606779 Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. file:human/KIF5B/KIF5B-uniprot.txt Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. |
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Download this section (compressed HTML)Q: Does direct starvation-recovery or autophagic lysosome reformation evidence support annotating KIF5B to a lysosome-organization/ALR term, or should KIF5B remain annotated to lysosome localization only?
Suggested experts: Sean Munro, Cleo T. Schiefermeier
Q: Should GO curation distinguish kinesin-1 heavy-chain core motor activity from cargo-specific downstream outcomes such as NK cytotoxicity, channel trafficking, and neuronal synaptic phenotypes?
Suggested experts: GO curator, kinesin-1 domain expert
Experiment: Measure KIF5B recruitment to autolysosomes and lysosomal tubules during starvation and nutrient refeeding, using endogenous tagging plus KIF5B depletion/rescue.
Hypothesis: KIF5B contributes to lysosome positioning during recovery but requires additional evidence before annotation to autophagic lysosome reformation or lysosome organization.
Type: live-cell imaging/genetic rescue
Experiment: Compare wild-type KIF5B and motor-dead or cargo-adaptor-binding mutants for lysosome localization, lytic granule polarization, and generic vesicle transport in matched cell systems.
Hypothesis: Cargo-specific KIF5B phenotypes depend on the same core plus-end-directed motor activity but separable adaptor interactions.
Type: structure-function perturbation
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