id: P33176
gene_symbol: KIF5B
product_type: PROTEIN
status: COMPLETE
taxon:
  id: NCBITaxon:9606
  label: Homo sapiens
description: >-
  KIF5B encodes the ubiquitous kinesin-1 heavy chain, an ATP-dependent, plus-end-directed microtubule
  motor. Its core molecular role is to form kinesin-1 motor complexes that bind microtubules and move
  vesicles and organelles toward microtubule plus ends. KIF5B is recruited to many cargos through
  adaptors, including Arl8/SKIP-dependent lysosome positioning, lytic granule polarization in natural
  killer cells, mitochondrial transport, neuronal cargo transport, centrosome/nuclear positioning, and
  channel trafficking. Its lysosomal role is kinesin-dependent lysosome localization/positioning
  through adaptor-mediated cargo recruitment.
existing_annotations:
- term:
    id: GO:0005871
    label: kinesin complex
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: part_of
  review:
    summary: KIF5B is a heavy-chain subunit of the kinesin-1 motor complex, which 
      contains two KIF5B heavy chains and kinesin light chains.
    action: ACCEPT
    reason: The cellular-component annotation reflects the canonical kinesin-1 complex 
      architecture.
    supported_by:
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: Oligomer composed of two heavy chains and two light chains.
- term:
    id: GO:0007411
    label: axon guidance
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  review:
    summary: This is a downstream neuronal phenotype or synaptic consequence inferred 
      for kinesin-family cargo transport rather than a direct molecular or cellular core
      role of KIF5B.
    action: MARK_AS_OVER_ANNOTATED
    reason: The direct KIF5B function is motor-driven cargo transport; annotating broad 
      neuronal outcomes as KIF5B functions overstates the evidence.
    proposed_replacement_terms:
    - id: GO:0047496
      label: vesicle transport along microtubule
- term:
    id: GO:0048489
    label: synaptic vesicle transport
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  review:
    summary: This is a plausible cargo- or cell-type-specific transport role for 
      kinesin-1, but it is not the defining KIF5B function.
    action: KEEP_AS_NON_CORE
    reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core 
      role is plus-end-directed microtubule cargo transport.
    supported_by:
    - reference_id: PMID:28426968
      supporting_text: 'distinct kinesins differentially regulate cargo velocity: kinesin-3
        drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport'
- term:
    id: GO:0098971
    label: anterograde dendritic transport of neurotransmitter receptor complex
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  review:
    summary: This is a plausible cargo- or cell-type-specific transport role for 
      kinesin-1, but it is not the defining KIF5B function.
    action: KEEP_AS_NON_CORE
    reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core 
      role is plus-end-directed microtubule cargo transport.
    supported_by:
    - reference_id: PMID:28426968
      supporting_text: 'distinct kinesins differentially regulate cargo velocity: kinesin-3
        drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport'
- term:
    id: GO:0008574
    label: plus-end-directed microtubule motor activity
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: enables
  review:
    summary: This is the most informative existing molecular-function annotation for 
      KIF5B. KIF5B is the kinesin-1 heavy chain and acts as a plus-end-directed 
      microtubule motor.
    action: ACCEPT
    reason: Retain as the core molecular function.
    supported_by:
    - reference_id: PMID:8606779
      supporting_text: Kinesin is the founding member of a superfamily of microtubule 
        based motor proteins that perform force-generating tasks such as organelle 
        transport and chromosome segregation.
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: Microtubule-dependent motor required for normal distribution of 
        mitochondria and lysosomes.
- term:
    id: GO:0003777
    label: microtubule motor activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  qualifier: enables
  review:
    summary: The annotation is correct but too broad for KIF5B, whose directional 
      kinesin-1 activity is better captured by plus-end-directed microtubule motor 
      activity.
    action: MODIFY
    reason: Replace the generic motor term with the specific plus-end-directed 
      microtubule motor term already present in GOA.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
    supported_by:
    - reference_id: PMID:8606779
      supporting_text: Kinesin is the founding member of a superfamily of microtubule 
        based motor proteins that perform force-generating tasks such as organelle 
        transport and chromosome segregation.
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: Microtubule-dependent motor required for normal distribution of 
        mitochondria and lysosomes.
- term:
    id: GO:0005524
    label: ATP binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: enables
  review:
    summary: The binding annotation is mechanistically true for kinesin-1 but less 
      informative than the full ATP-dependent motor activity.
    action: MODIFY
    reason: Use the specific plus-end-directed microtubule motor activity rather than 
      separate generic ATP-binding or microtubule-binding terms as the reviewed 
      molecular function.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
    supported_by:
    - reference_id: PMID:1607388
      supporting_text: the kinesin heavy chain showed both a diffuse distribution and a 
        filamentous staining pattern that coaligned with microtubules
    - reference_id: PMID:8606779
      supporting_text: Here, we report the crystal structure of the human kinesin motor 
        domain with bound ADP determined to 1.8-A resolution by X-ray crystallography.
- term:
    id: GO:0005765
    label: lysosomal membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  review:
    summary: KIF5B is recruited to the cytosolic face of lysosomal membranes through 
      adaptor systems such as Arl8/SKIP to drive lysosome positioning.
    action: KEEP_AS_NON_CORE
    reason: Keep as a supported PN-relevant cargo-specific localization, but do not 
      treat lysosomal membrane association as the defining location of KIF5B activity 
      across all cargos.
    supported_by:
    - reference_id: PMID:22172677
      supporting_text: endogenous KIF5B, the heavy chain of kinesin-1, accumulated on 
        lysosomes in cells overexpressing Arl8b and SKIP
    - reference_id: PMID:22172677
      supporting_text: when KIF5B was knocked down by >85% by RNAi, lysosomes were more 
        centrally clustered
- term:
    id: GO:0005856
    label: cytoskeleton
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  review:
    summary: KIF5B is a cytoskeleton-associated microtubule motor. The term is broad but
      consistent with the core motor mechanism.
    action: ACCEPT
    reason: Retain as a core cellular context for KIF5B motor activity.
    supported_by:
    - reference_id: PMID:1607388
      supporting_text: the kinesin heavy chain showed both a diffuse distribution and a 
        filamentous staining pattern that coaligned with microtubules
    - reference_id: PMID:8606779
      supporting_text: Here, we report the crystal structure of the human kinesin motor 
        domain with bound ADP determined to 1.8-A resolution by X-ray crystallography.
- term:
    id: GO:0007018
    label: microtubule-based movement
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: involved_in
  review:
    summary: Microtubule-based movement is correct but broad. KIF5B specifically 
      functions as a kinesin-1 cargo motor, so vesicle transport along microtubule is 
      the better process term.
    action: MODIFY
    reason: Replace the broad process with the cargo-transport process already supported
      in GOA.
    proposed_replacement_terms:
    - id: GO:0047496
      label: vesicle transport along microtubule
    supported_by:
    - reference_id: PMID:8606779
      supporting_text: Kinesin is the founding member of a superfamily of microtubule 
        based motor proteins that perform force-generating tasks such as organelle 
        transport and chromosome segregation.
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: Microtubule-dependent motor required for normal distribution of 
        mitochondria and lysosomes.
- term:
    id: GO:0008017
    label: microtubule binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: enables
  review:
    summary: The binding annotation is mechanistically true for kinesin-1 but less 
      informative than the full ATP-dependent motor activity.
    action: MODIFY
    reason: Use the specific plus-end-directed microtubule motor activity rather than 
      separate generic ATP-binding or microtubule-binding terms as the reviewed 
      molecular function.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
    supported_by:
    - reference_id: PMID:1607388
      supporting_text: the kinesin heavy chain showed both a diffuse distribution and a 
        filamentous staining pattern that coaligned with microtubules
    - reference_id: PMID:8606779
      supporting_text: Here, we report the crystal structure of the human kinesin motor 
        domain with bound ADP determined to 1.8-A resolution by X-ray crystallography.
- term:
    id: GO:0032839
    label: dendrite cytoplasm
  evidence_type: IEA
  original_reference_id: GO_REF:0000108
  qualifier: located_in
  review:
    summary: This neuronal compartment annotation is consistent with kinesin cargo 
      transport in neurons but is not a core localization for the ubiquitous KIF5B 
      motor.
    action: KEEP_AS_NON_CORE
    reason: Keep as a transferred, non-core neuronal localization context.
- term:
    id: GO:0101004
    label: cytolytic granule membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  review:
    summary: KIF5B participates in NK-cell lytic granule polarization and cytotoxicity 
      through an Arl8b/SKIP/kinesin-1 cargo-transport pathway.
    action: KEEP_AS_NON_CORE
    reason: Keep as a well-supported immune-cell cargo-specific role, secondary to the 
      core kinesin motor function.
    supported_by:
    - reference_id: PMID:24088571
      supporting_text: We also identified four unique peptides corresponding to KIF5B 
        (the heavy chain of the plus-end motor protein kinesin-1 [KHC]) in the Arl8b 
        pull down
    - reference_id: PMID:24088571
      supporting_text: Silencing of KIF5B expression inhibited the polarization of both 
        the MTOC and lytic granules to the immune synapse
- term:
    id: GO:1904115
    label: axon cytoplasm
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  qualifier: located_in
  review:
    summary: This neuronal compartment annotation is consistent with kinesin cargo 
      transport in neurons but is not a core localization for the ubiquitous KIF5B 
      motor.
    action: KEEP_AS_NON_CORE
    reason: Keep as a transferred, non-core neuronal localization context.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:15161933
  qualifier: enables
  review:
    summary: The interaction may be experimentally observed, but the GO term is generic 
      and does not convey KIF5B biology.
    action: MARK_AS_OVER_ANNOTATED
    reason: Do not use generic binding terms as reviewed KIF5B function; the informative
      molecular function is plus-end-directed microtubule motor activity and cargo 
      transport.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:19164528
  qualifier: enables
  review:
    summary: The interaction may be experimentally observed, but the GO term is generic 
      and does not convey KIF5B biology.
    action: MARK_AS_OVER_ANNOTATED
    reason: Do not use generic binding terms as reviewed KIF5B function; the informative
      molecular function is plus-end-directed microtubule motor activity and cargo 
      transport.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:20562859
  qualifier: enables
  review:
    summary: The interaction may be experimentally observed, but the GO term is generic 
      and does not convey KIF5B biology.
    action: MARK_AS_OVER_ANNOTATED
    reason: Do not use generic binding terms as reviewed KIF5B function; the informative
      molecular function is plus-end-directed microtubule motor activity and cargo 
      transport.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:21915095
  qualifier: enables
  review:
    summary: The interaction may be experimentally observed, but the GO term is generic 
      and does not convey KIF5B biology.
    action: MARK_AS_OVER_ANNOTATED
    reason: Do not use generic binding terms as reviewed KIF5B function; the informative
      molecular function is plus-end-directed microtubule motor activity and cargo 
      transport.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:25855459
  qualifier: enables
  review:
    summary: The interaction may be experimentally observed, but the GO term is generic 
      and does not convey KIF5B biology.
    action: MARK_AS_OVER_ANNOTATED
    reason: Do not use generic binding terms as reviewed KIF5B function; the informative
      molecular function is plus-end-directed microtubule motor activity and cargo 
      transport.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:28514442
  qualifier: enables
  review:
    summary: The interaction may be experimentally observed, but the GO term is generic 
      and does not convey KIF5B biology.
    action: MARK_AS_OVER_ANNOTATED
    reason: Do not use generic binding terms as reviewed KIF5B function; the informative
      molecular function is plus-end-directed microtubule motor activity and cargo 
      transport.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:32296183
  qualifier: enables
  review:
    summary: The interaction may be experimentally observed, but the GO term is generic 
      and does not convey KIF5B biology.
    action: MARK_AS_OVER_ANNOTATED
    reason: Do not use generic binding terms as reviewed KIF5B function; the informative
      molecular function is plus-end-directed microtubule motor activity and cargo 
      transport.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:33961781
  qualifier: enables
  review:
    summary: The interaction may be experimentally observed, but the GO term is generic 
      and does not convey KIF5B biology.
    action: MARK_AS_OVER_ANNOTATED
    reason: Do not use generic binding terms as reviewed KIF5B function; the informative
      molecular function is plus-end-directed microtubule motor activity and cargo 
      transport.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:35271311
  qualifier: enables
  review:
    summary: The interaction may be experimentally observed, but the GO term is generic 
      and does not convey KIF5B biology.
    action: MARK_AS_OVER_ANNOTATED
    reason: Do not use generic binding terms as reviewed KIF5B function; the informative
      molecular function is plus-end-directed microtubule motor activity and cargo 
      transport.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:36931259
  qualifier: enables
  review:
    summary: The interaction may be experimentally observed, but the GO term is generic 
      and does not convey KIF5B biology.
    action: MARK_AS_OVER_ANNOTATED
    reason: Do not use generic binding terms as reviewed KIF5B function; the informative
      molecular function is plus-end-directed microtubule motor activity and cargo 
      transport.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
- term:
    id: GO:0042802
    label: identical protein binding
  evidence_type: IPI
  original_reference_id: PMID:17013387
  qualifier: enables
  review:
    summary: The interaction may be experimentally observed, but the GO term is generic 
      and does not convey KIF5B biology.
    action: MARK_AS_OVER_ANNOTATED
    reason: Do not use generic binding terms as reviewed KIF5B function; the informative
      molecular function is plus-end-directed microtubule motor activity and cargo 
      transport.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
- term:
    id: GO:0042802
    label: identical protein binding
  evidence_type: IPI
  original_reference_id: PMID:18004302
  qualifier: enables
  review:
    summary: The interaction may be experimentally observed, but the GO term is generic 
      and does not convey KIF5B biology.
    action: MARK_AS_OVER_ANNOTATED
    reason: Do not use generic binding terms as reviewed KIF5B function; the informative
      molecular function is plus-end-directed microtubule motor activity and cargo 
      transport.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: located_in
  review:
    summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization
      does not describe the specific motor function.
    action: KEEP_AS_NON_CORE
    reason: Keep as a correct broad location, not as a core functional annotation.
    supported_by:
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton'
- term:
    id: GO:0005739
    label: mitochondrion
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: located_in
  review:
    summary: KIF5B can associate with diverse organelles or cellular regions through 
      cargo adaptors, but this term is a cargo/context-specific location rather than the
      core motor role.
    action: KEEP_AS_NON_CORE
    reason: Keep as non-core for a pleiotropic motor with many cargo associations.
- term:
    id: GO:0030139
    label: endocytic vesicle
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: located_in
  review:
    summary: KIF5B can associate with diverse organelles or cellular regions through 
      cargo adaptors, but this term is a cargo/context-specific location rather than the
      core motor role.
    action: KEEP_AS_NON_CORE
    reason: Keep as non-core for a pleiotropic motor with many cargo associations.
- term:
    id: GO:0032230
    label: positive regulation of synaptic transmission, GABAergic
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: involved_in
  review:
    summary: This is a downstream neuronal phenotype or synaptic consequence inferred 
      for kinesin-family cargo transport rather than a direct molecular or cellular core
      role of KIF5B.
    action: MARK_AS_OVER_ANNOTATED
    reason: The direct KIF5B function is motor-driven cargo transport; annotating broad 
      neuronal outcomes as KIF5B functions overstates the evidence.
    proposed_replacement_terms:
    - id: GO:0047496
      label: vesicle transport along microtubule
- term:
    id: GO:0035253
    label: ciliary rootlet
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: located_in
  review:
    summary: KIF5B can associate with diverse organelles or cellular regions through 
      cargo adaptors, but this term is a cargo/context-specific location rather than the
      core motor role.
    action: KEEP_AS_NON_CORE
    reason: Keep as non-core for a pleiotropic motor with many cargo associations.
- term:
    id: GO:0035617
    label: stress granule disassembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: involved_in
  review:
    summary: This is a plausible cargo- or cell-type-specific transport role for 
      kinesin-1, but it is not the defining KIF5B function.
    action: KEEP_AS_NON_CORE
    reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core 
      role is plus-end-directed microtubule cargo transport.
    supported_by:
    - reference_id: PMID:28426968
      supporting_text: 'distinct kinesins differentially regulate cargo velocity: kinesin-3
        drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport'
- term:
    id: GO:0043005
    label: neuron projection
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: located_in
  review:
    summary: This neuronal compartment annotation is consistent with kinesin cargo 
      transport in neurons but is not a core localization for the ubiquitous KIF5B 
      motor.
    action: KEEP_AS_NON_CORE
    reason: Keep as a transferred, non-core neuronal localization context.
- term:
    id: GO:0044877
    label: protein-containing complex binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: enables
  review:
    summary: The interaction may be experimentally observed, but the GO term is generic 
      and does not convey KIF5B biology.
    action: MARK_AS_OVER_ANNOTATED
    reason: Do not use generic binding terms as reviewed KIF5B function; the informative
      molecular function is plus-end-directed microtubule motor activity and cargo 
      transport.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
- term:
    id: GO:0045335
    label: phagocytic vesicle
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: located_in
  review:
    summary: KIF5B can associate with diverse organelles or cellular regions through 
      cargo adaptors, but this term is a cargo/context-specific location rather than the
      core motor role.
    action: KEEP_AS_NON_CORE
    reason: Keep as non-core for a pleiotropic motor with many cargo associations.
- term:
    id: GO:0098987
    label: regulation of modification of synapse structure, modulating synaptic 
      transmission
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: involved_in
  review:
    summary: This is a downstream neuronal phenotype or synaptic consequence inferred 
      for kinesin-family cargo transport rather than a direct molecular or cellular core
      role of KIF5B.
    action: MARK_AS_OVER_ANNOTATED
    reason: The direct KIF5B function is motor-driven cargo transport; annotating broad 
      neuronal outcomes as KIF5B functions overstates the evidence.
    proposed_replacement_terms:
    - id: GO:0047496
      label: vesicle transport along microtubule
- term:
    id: GO:0099524
    label: postsynaptic cytosol
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: is_active_in
  review:
    summary: This seeded annotation was reviewed as a non-core KIF5B context.
    action: KEEP_AS_NON_CORE
    reason: KIF5B is a pleiotropic microtubule motor; this term is retained only as a 
      secondary context.
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: IDA
  original_reference_id: GO_REF:0000052
  qualifier: located_in
  review:
    summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization
      does not describe the specific motor function.
    action: KEEP_AS_NON_CORE
    reason: Keep as a correct broad location, not as a core functional annotation.
    supported_by:
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton'
- term:
    id: GO:0005765
    label: lysosomal membrane
  evidence_type: EXP
  original_reference_id: PMID:22172677
  qualifier: located_in
  review:
    summary: KIF5B is recruited to the cytosolic face of lysosomal membranes through 
      adaptor systems such as Arl8/SKIP to drive lysosome positioning.
    action: KEEP_AS_NON_CORE
    reason: Keep as a supported PN-relevant cargo-specific localization, but do not 
      treat lysosomal membrane association as the defining location of KIF5B activity 
      across all cargos.
    supported_by:
    - reference_id: PMID:22172677
      supporting_text: endogenous KIF5B, the heavy chain of kinesin-1, accumulated on 
        lysosomes in cells overexpressing Arl8b and SKIP
    - reference_id: PMID:22172677
      supporting_text: when KIF5B was knocked down by >85% by RNAi, lysosomes were more 
        centrally clustered
- term:
    id: GO:0005765
    label: lysosomal membrane
  evidence_type: EXP
  original_reference_id: PMID:24088571
  qualifier: located_in
  review:
    summary: KIF5B is recruited to the cytosolic face of lysosomal membranes through 
      adaptor systems such as Arl8/SKIP to drive lysosome positioning.
    action: KEEP_AS_NON_CORE
    reason: Keep as a supported PN-relevant cargo-specific localization, but do not 
      treat lysosomal membrane association as the defining location of KIF5B activity 
      across all cargos.
    supported_by:
    - reference_id: PMID:22172677
      supporting_text: endogenous KIF5B, the heavy chain of kinesin-1, accumulated on 
        lysosomes in cells overexpressing Arl8b and SKIP
    - reference_id: PMID:22172677
      supporting_text: when KIF5B was knocked down by >85% by RNAi, lysosomes were more 
        centrally clustered
- term:
    id: GO:0101004
    label: cytolytic granule membrane
  evidence_type: EXP
  original_reference_id: PMID:24088571
  qualifier: located_in
  review:
    summary: KIF5B participates in NK-cell lytic granule polarization and cytotoxicity 
      through an Arl8b/SKIP/kinesin-1 cargo-transport pathway.
    action: KEEP_AS_NON_CORE
    reason: Keep as a well-supported immune-cell cargo-specific role, secondary to the 
      core kinesin motor function.
    supported_by:
    - reference_id: PMID:24088571
      supporting_text: We also identified four unique peptides corresponding to KIF5B 
        (the heavy chain of the plus-end motor protein kinesin-1 [KHC]) in the Arl8b 
        pull down
    - reference_id: PMID:24088571
      supporting_text: Silencing of KIF5B expression inhibited the polarization of both 
        the MTOC and lytic granules to the immune synapse
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9700181
  qualifier: located_in
  review:
    summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization
      does not describe the specific motor function.
    action: KEEP_AS_NON_CORE
    reason: Keep as a correct broad location, not as a core functional annotation.
    supported_by:
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton'
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9712078
  qualifier: located_in
  review:
    summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization
      does not describe the specific motor function.
    action: KEEP_AS_NON_CORE
    reason: Keep as a correct broad location, not as a core functional annotation.
    supported_by:
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton'
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9712079
  qualifier: located_in
  review:
    summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization
      does not describe the specific motor function.
    action: KEEP_AS_NON_CORE
    reason: Keep as a correct broad location, not as a core functional annotation.
    supported_by:
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton'
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9712083
  qualifier: located_in
  review:
    summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization
      does not describe the specific motor function.
    action: KEEP_AS_NON_CORE
    reason: Keep as a correct broad location, not as a core functional annotation.
    supported_by:
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton'
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9712084
  qualifier: located_in
  review:
    summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization
      does not describe the specific motor function.
    action: KEEP_AS_NON_CORE
    reason: Keep as a correct broad location, not as a core functional annotation.
    supported_by:
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton'
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9712085
  qualifier: located_in
  review:
    summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization
      does not describe the specific motor function.
    action: KEEP_AS_NON_CORE
    reason: Keep as a correct broad location, not as a core functional annotation.
    supported_by:
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton'
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9850958
  qualifier: located_in
  review:
    summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization
      does not describe the specific motor function.
    action: KEEP_AS_NON_CORE
    reason: Keep as a correct broad location, not as a core functional annotation.
    supported_by:
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton'
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:28426968
  qualifier: enables
  review:
    summary: The interaction may be experimentally observed, but the GO term is generic 
      and does not convey KIF5B biology.
    action: MARK_AS_OVER_ANNOTATED
    reason: Do not use generic binding terms as reviewed KIF5B function; the informative
      molecular function is plus-end-directed microtubule motor activity and cargo 
      transport.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
- term:
    id: GO:0042267
    label: natural killer cell mediated cytotoxicity
  evidence_type: IMP
  original_reference_id: PMID:24088571
  qualifier: involved_in
  review:
    summary: KIF5B participates in NK-cell lytic granule polarization and cytotoxicity 
      through an Arl8b/SKIP/kinesin-1 cargo-transport pathway.
    action: KEEP_AS_NON_CORE
    reason: Keep as a well-supported immune-cell cargo-specific role, secondary to the 
      core kinesin motor function.
    supported_by:
    - reference_id: PMID:24088571
      supporting_text: We also identified four unique peptides corresponding to KIF5B 
        (the heavy chain of the plus-end motor protein kinesin-1 [KHC]) in the Arl8b 
        pull down
    - reference_id: PMID:24088571
      supporting_text: Silencing of KIF5B expression inhibited the polarization of both 
        the MTOC and lytic granules to the immune synapse
- term:
    id: GO:0032418
    label: lysosome localization
  evidence_type: IMP
  original_reference_id: PMID:22172677
  qualifier: involved_in
  review:
    summary: KIF5B has direct evidence for lysosome positioning/localization through 
      Arl8/SKIP-dependent recruitment of kinesin-1 to lysosomes.
    action: KEEP_AS_NON_CORE
    reason: Keep as the supported Proteostasis Network-relevant cargo-specific process. 
      This does not justify propagating the broader PN lysosomal-tubulation context to 
      lysosome organization.
    supported_by:
    - reference_id: PMID:22172677
      supporting_text: endogenous KIF5B, the heavy chain of kinesin-1, accumulated on 
        lysosomes in cells overexpressing Arl8b and SKIP
    - reference_id: PMID:22172677
      supporting_text: when KIF5B was knocked down by >85% by RNAi, lysosomes were more 
        centrally clustered
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-9700179
  qualifier: located_in
  review:
    summary: KIF5B is a cytosolic/cytoplasmic motor protein, but this broad localization
      does not describe the specific motor function.
    action: KEEP_AS_NON_CORE
    reason: Keep as a correct broad location, not as a core functional annotation.
    supported_by:
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton'
- term:
    id: GO:0099641
    label: anterograde axonal protein transport
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  qualifier: involved_in
  review:
    summary: This is a plausible cargo- or cell-type-specific transport role for 
      kinesin-1, but it is not the defining KIF5B function.
    action: KEEP_AS_NON_CORE
    reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core 
      role is plus-end-directed microtubule cargo transport.
    supported_by:
    - reference_id: PMID:28426968
      supporting_text: 'distinct kinesins differentially regulate cargo velocity: kinesin-3
        drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport'
- term:
    id: GO:0045296
    label: cadherin binding
  evidence_type: HDA
  original_reference_id: PMID:25468996
  qualifier: enables
  review:
    summary: The interaction may be experimentally observed, but the GO term is generic 
      and does not convey KIF5B biology.
    action: MARK_AS_OVER_ANNOTATED
    reason: Do not use generic binding terms as reviewed KIF5B function; the informative
      molecular function is plus-end-directed microtubule motor activity and cargo 
      transport.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
- term:
    id: GO:0003777
    label: microtubule motor activity
  evidence_type: IDA
  original_reference_id: PMID:28426968
  qualifier: enables
  review:
    summary: The annotation is correct but too broad for KIF5B, whose directional 
      kinesin-1 activity is better captured by plus-end-directed microtubule motor 
      activity.
    action: MODIFY
    reason: Replace the generic motor term with the specific plus-end-directed 
      microtubule motor term already present in GOA.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
    supported_by:
    - reference_id: PMID:8606779
      supporting_text: Kinesin is the founding member of a superfamily of microtubule 
        based motor proteins that perform force-generating tasks such as organelle 
        transport and chromosome segregation.
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: Microtubule-dependent motor required for normal distribution of 
        mitochondria and lysosomes.
- term:
    id: GO:0008017
    label: microtubule binding
  evidence_type: IDA
  original_reference_id: PMID:28426968
  qualifier: enables
  review:
    summary: The binding annotation is mechanistically true for kinesin-1 but less 
      informative than the full ATP-dependent motor activity.
    action: MODIFY
    reason: Use the specific plus-end-directed microtubule motor activity rather than 
      separate generic ATP-binding or microtubule-binding terms as the reviewed 
      molecular function.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
    supported_by:
    - reference_id: PMID:1607388
      supporting_text: the kinesin heavy chain showed both a diffuse distribution and a 
        filamentous staining pattern that coaligned with microtubules
    - reference_id: PMID:8606779
      supporting_text: Here, we report the crystal structure of the human kinesin motor 
        domain with bound ADP determined to 1.8-A resolution by X-ray crystallography.
- term:
    id: GO:0047496
    label: vesicle transport along microtubule
  evidence_type: IDA
  original_reference_id: PMID:28426968
  qualifier: involved_in
  review:
    summary: This process captures the core cargo-transport role of KIF5B as a kinesin-1
      motor moving vesicles and organelles along microtubules.
    action: ACCEPT
    reason: Retain as a core biological-process abstraction for KIF5B motor function.
    supported_by:
    - reference_id: PMID:24088571
      supporting_text: Kinesin-1 regulates long-range anterograde intracellular 
        transport of vesicles, including lysosomes
- term:
    id: GO:1990048
    label: anterograde neuronal dense core vesicle transport
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  qualifier: involved_in
  review:
    summary: This is a plausible cargo- or cell-type-specific transport role for 
      kinesin-1, but it is not the defining KIF5B function.
    action: KEEP_AS_NON_CORE
    reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core 
      role is plus-end-directed microtubule cargo transport.
    supported_by:
    - reference_id: PMID:28426968
      supporting_text: 'distinct kinesins differentially regulate cargo velocity: kinesin-3
        drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport'
- term:
    id: GO:1990049
    label: retrograde neuronal dense core vesicle transport
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  qualifier: involved_in
  review:
    summary: This is a plausible cargo- or cell-type-specific transport role for 
      kinesin-1, but it is not the defining KIF5B function.
    action: KEEP_AS_NON_CORE
    reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core 
      role is plus-end-directed microtubule cargo transport.
    supported_by:
    - reference_id: PMID:28426968
      supporting_text: 'distinct kinesins differentially regulate cargo velocity: kinesin-3
        drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport'
- term:
    id: GO:0051642
    label: centrosome localization
  evidence_type: IMP
  original_reference_id: PMID:20386726
  qualifier: involved_in
  review:
    summary: KIF5B contributes to centrosome and nuclear positioning during mitotic 
      entry through antagonism with dynein-dependent forces.
    action: KEEP_AS_NON_CORE
    reason: Keep as a supported cell-cycle positioning role, but not as the core 
      function of this pleiotropic motor.
    supported_by:
    - reference_id: PMID:20386726
      supporting_text: we show that while dynein pulls centrosomes and the nucleus 
        together during mitotic entry, kinesin-1 pushes them apart
- term:
    id: GO:0016020
    label: membrane
  evidence_type: HDA
  original_reference_id: PMID:19946888
  qualifier: located_in
  review:
    summary: Broad membrane is a high-throughput location that loses the important 
      biology. KIF5B is a peripheral/adaptor-recruited motor on specific cargos such as 
      lysosomes and lytic granules, not a generic membrane protein.
    action: MARK_AS_OVER_ANNOTATED
    reason: Retain the more specific cargo-membrane annotations where supported and 
      avoid the uninformative broad membrane term.
    proposed_replacement_terms:
    - id: GO:0005765
      label: lysosomal membrane
    - id: GO:0101004
      label: cytolytic granule membrane
- term:
    id: GO:0042391
    label: regulation of membrane potential
  evidence_type: IDA
  original_reference_id: PMID:19675065
  qualifier: involved_in
  review:
    summary: These annotations describe downstream electrophysiological consequences of 
      KIF5B-dependent Kv1.5 channel trafficking rather than the direct KIF5B process.
    action: MODIFY
    reason: Replace membrane-potential/potassium-transport regulation with the more 
      direct protein-localization-to-plasma-membrane process supported by the original 
      study.
    proposed_replacement_terms:
    - id: GO:1903078
      label: positive regulation of protein localization to plasma membrane
    supported_by:
    - reference_id: PMID:19675065
      supporting_text: Thus, Kif5b is essential to anterograde trafficking of a cardiac 
        voltage-gated potassium channel.
- term:
    id: GO:0043268
    label: positive regulation of potassium ion transport
  evidence_type: IDA
  original_reference_id: PMID:19675065
  qualifier: involved_in
  review:
    summary: These annotations describe downstream electrophysiological consequences of 
      KIF5B-dependent Kv1.5 channel trafficking rather than the direct KIF5B process.
    action: MODIFY
    reason: Replace membrane-potential/potassium-transport regulation with the more 
      direct protein-localization-to-plasma-membrane process supported by the original 
      study.
    proposed_replacement_terms:
    - id: GO:1903078
      label: positive regulation of protein localization to plasma membrane
    supported_by:
    - reference_id: PMID:19675065
      supporting_text: Thus, Kif5b is essential to anterograde trafficking of a cardiac 
        voltage-gated potassium channel.
- term:
    id: GO:1903078
    label: positive regulation of protein localization to plasma membrane
  evidence_type: IDA
  original_reference_id: PMID:19675065
  qualifier: involved_in
  review:
    summary: 'This captures the direct mechanism in the Kv1.5 study: KIF5B promotes forward
      trafficking and surface localization of the channel.'
    action: KEEP_AS_NON_CORE
    reason: Keep as a supported cargo-specific channel-trafficking role, secondary to 
      the core microtubule motor function.
    supported_by:
    - reference_id: PMID:19675065
      supporting_text: Thus, Kif5b is essential to anterograde trafficking of a cardiac 
        voltage-gated potassium channel.
- term:
    id: GO:0031982
    label: vesicle
  evidence_type: IDA
  original_reference_id: PMID:20682791
  qualifier: located_in
  review:
    summary: KIF5B can associate with diverse organelles or cellular regions through 
      cargo adaptors, but this term is a cargo/context-specific location rather than the
      core motor role.
    action: KEEP_AS_NON_CORE
    reason: Keep as non-core for a pleiotropic motor with many cargo associations.
- term:
    id: GO:0035617
    label: stress granule disassembly
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  qualifier: involved_in
  review:
    summary: This is a plausible cargo- or cell-type-specific transport role for 
      kinesin-1, but it is not the defining KIF5B function.
    action: KEEP_AS_NON_CORE
    reason: Keep as non-core because KIF5B is a pleiotropic motor whose conserved core 
      role is plus-end-directed microtubule cargo transport.
    supported_by:
    - reference_id: PMID:28426968
      supporting_text: 'distinct kinesins differentially regulate cargo velocity: kinesin-3
        drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport'
- term:
    id: GO:0003777
    label: microtubule motor activity
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  qualifier: enables
  review:
    summary: The annotation is correct but too broad for KIF5B, whose directional 
      kinesin-1 activity is better captured by plus-end-directed microtubule motor 
      activity.
    action: MODIFY
    reason: Replace the generic motor term with the specific plus-end-directed 
      microtubule motor term already present in GOA.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
    supported_by:
    - reference_id: PMID:8606779
      supporting_text: Kinesin is the founding member of a superfamily of microtubule 
        based motor proteins that perform force-generating tasks such as organelle 
        transport and chromosome segregation.
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: Microtubule-dependent motor required for normal distribution of 
        mitochondria and lysosomes.
- term:
    id: GO:0008017
    label: microtubule binding
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  qualifier: enables
  review:
    summary: The binding annotation is mechanistically true for kinesin-1 but less 
      informative than the full ATP-dependent motor activity.
    action: MODIFY
    reason: Use the specific plus-end-directed microtubule motor activity rather than 
      separate generic ATP-binding or microtubule-binding terms as the reviewed 
      molecular function.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
    supported_by:
    - reference_id: PMID:1607388
      supporting_text: the kinesin heavy chain showed both a diffuse distribution and a 
        filamentous staining pattern that coaligned with microtubules
    - reference_id: PMID:8606779
      supporting_text: Here, we report the crystal structure of the human kinesin motor 
        domain with bound ADP determined to 1.8-A resolution by X-ray crystallography.
- term:
    id: GO:0047496
    label: vesicle transport along microtubule
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  qualifier: involved_in
  review:
    summary: This process captures the core cargo-transport role of KIF5B as a kinesin-1
      motor moving vesicles and organelles along microtubules.
    action: ACCEPT
    reason: Retain as a core biological-process abstraction for KIF5B motor function.
    supported_by:
    - reference_id: PMID:24088571
      supporting_text: Kinesin-1 regulates long-range anterograde intracellular 
        transport of vesicles, including lysosomes
- term:
    id: GO:0048471
    label: perinuclear region of cytoplasm
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  qualifier: located_in
  review:
    summary: KIF5B can associate with diverse organelles or cellular regions through 
      cargo adaptors, but this term is a cargo/context-specific location rather than the
      core motor role.
    action: KEEP_AS_NON_CORE
    reason: Keep as non-core for a pleiotropic motor with many cargo associations.
- term:
    id: GO:0003777
    label: microtubule motor activity
  evidence_type: TAS
  original_reference_id: PMID:8606779
  qualifier: enables
  review:
    summary: The annotation is correct but too broad for KIF5B, whose directional 
      kinesin-1 activity is better captured by plus-end-directed microtubule motor 
      activity.
    action: MODIFY
    reason: Replace the generic motor term with the specific plus-end-directed 
      microtubule motor term already present in GOA.
    proposed_replacement_terms:
    - id: GO:0008574
      label: plus-end-directed microtubule motor activity
    supported_by:
    - reference_id: PMID:8606779
      supporting_text: Kinesin is the founding member of a superfamily of microtubule 
        based motor proteins that perform force-generating tasks such as organelle 
        transport and chromosome segregation.
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: Microtubule-dependent motor required for normal distribution of 
        mitochondria and lysosomes.
- term:
    id: GO:0005871
    label: kinesin complex
  evidence_type: TAS
  original_reference_id: PMID:1607388
  qualifier: part_of
  review:
    summary: KIF5B is a heavy-chain subunit of the kinesin-1 motor complex, which 
      contains two KIF5B heavy chains and kinesin light chains.
    action: ACCEPT
    reason: The cellular-component annotation reflects the canonical kinesin-1 complex 
      architecture.
    supported_by:
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: Oligomer composed of two heavy chains and two light chains.
- term:
    id: GO:0007018
    label: microtubule-based movement
  evidence_type: TAS
  original_reference_id: PMID:7514426
  qualifier: involved_in
  review:
    summary: Microtubule-based movement is correct but broad. KIF5B specifically 
      functions as a kinesin-1 cargo motor, so vesicle transport along microtubule is 
      the better process term.
    action: MODIFY
    reason: Replace the broad process with the cargo-transport process already supported
      in GOA.
    proposed_replacement_terms:
    - id: GO:0047496
      label: vesicle transport along microtubule
    supported_by:
    - reference_id: PMID:8606779
      supporting_text: Kinesin is the founding member of a superfamily of microtubule 
        based motor proteins that perform force-generating tasks such as organelle 
        transport and chromosome segregation.
    - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
      supporting_text: Microtubule-dependent motor required for normal distribution of 
        mitochondria and lysosomes.
references:
- id: GO_REF:0000002
  title: Gene Ontology annotation through association of InterPro records with GO terms
  findings: []
- id: GO_REF:0000024
  title: Manual transfer of experimentally-verified manual GO annotation data to 
    orthologs by curator judgment of sequence similarity
  findings: []
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
- id: GO_REF:0000044
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location 
    vocabulary mapping, accompanied by conservative changes to GO terms applied by 
    UniProt
  findings: []
- id: GO_REF:0000052
  title: Gene Ontology annotation based on curation of immunofluorescence data
  findings: []
- id: GO_REF:0000107
  title: Automatic transfer of experimentally verified manual GO annotation data to 
    orthologs using Ensembl Compara
  findings: []
- id: GO_REF:0000108
  title: Automatic assignment of GO terms using logical inference, based on on 
    inter-ontology links
  findings: []
- id: GO_REF:0000120
  title: Combined Automated Annotation using Multiple IEA Methods
  findings: []
- id: PMID:15161933
  title: Comprehensive proteomic analysis of interphase and mitotic 14-3-3-binding 
    proteins.
  findings: []
- id: PMID:1607388
  title: 'Cloning and expression of a human kinesin heavy chain gene: interaction of the COOH-terminal
    domain with cytoplasmic microtubules in transfected CV-1 cells.'
  findings: []
- id: PMID:17013387
  title: Single-molecule observations of neck linker conformational changes in the 
    kinesin motor protein.
  findings: []
- id: PMID:18004302
  title: How kinesin waits between steps.
  findings: []
- id: PMID:19164528
  title: Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated
    cell polarization.
  findings: []
- id: PMID:19675065
  title: Kif5b is an essential forward trafficking motor for the Kv1.5 cardiac potassium
    channel.
  findings: []
- id: PMID:19946888
  title: Defining the membrane proteome of NK cells.
  findings: []
- id: PMID:20386726
  title: Bicaudal D2, dynein, and kinesin-1 associate with nuclear pore complexes and 
    regulate centrosome and nuclear positioning during mitotic entry.
  findings: []
- id: PMID:20562859
  title: Network organization of the human autophagy system.
  findings: []
- id: PMID:20682791
  title: A protein interaction network for Ecm29 links the 26 S proteasome to molecular 
    motors and endosomal components.
  findings: []
- id: PMID:21915095
  title: A kinesin-1 binding motif in vaccinia virus that is widespread throughout the 
    human genome.
  findings: []
- id: PMID:22172677
  title: Arl8 and SKIP act together to link lysosomes to kinesin-1.
  findings: []
- id: PMID:24088571
  title: Arf-like GTPase Arl8b regulates lytic granule polarization and natural killer 
    cell-mediated cytotoxicity.
  findings: []
- id: PMID:25468996
  title: E-cadherin interactome complexity and robustness resolved by quantitative 
    proteomics.
  findings: []
- id: PMID:25855459
  title: Repeated ER-endosome contacts promote endosome translocation and neurite 
    outgrowth.
  findings: []
- id: PMID:28426968
  title: MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus 
    KIF5-Dependent Cargo Transport in Sensory Neurons.
  findings: []
- id: PMID:28514442
  title: Architecture of the human interactome defines protein communities and disease 
    networks.
  findings: []
- id: PMID:32296183
  title: A reference map of the human binary protein interactome.
  findings: []
- id: PMID:33961781
  title: Dual proteome-scale networks reveal cell-specific remodeling of the human 
    interactome.
  findings: []
- id: PMID:35271311
  title: 'OpenCell: Endogenous tagging for the cartography of human cellular organization.'
  findings: []
- id: PMID:36931259
  title: A central chaperone-like role for 14-3-3 proteins in human cells.
  findings: []
- id: PMID:7514426
  title: Cloning and localization of a conventional kinesin motor expressed exclusively 
    in neurons.
  findings: []
- id: PMID:8606779
  title: Crystal structure of the kinesin motor domain reveals a structural similarity 
    to myosin.
  findings: []
- id: Reactome:R-HSA-9700179
  title: Ligand-independent dimerization of ALK fusions
  findings: []
- id: Reactome:R-HSA-9700181
  title: Autophosphorylation of ALK fusions
  findings: []
- id: Reactome:R-HSA-9712078
  title: ALK mutants bind PI3KR1
  findings: []
- id: Reactome:R-HSA-9712079
  title: ALK mutants bind STAT3
  findings: []
- id: Reactome:R-HSA-9712083
  title: ALK mutants bind PI3KCA
  findings: []
- id: Reactome:R-HSA-9712084
  title: PI3K synthesizes PIP3 downstream of ALK mutants
  findings: []
- id: Reactome:R-HSA-9712085
  title: ALK mutants phosphorylate STAT3
  findings: []
- id: Reactome:R-HSA-9850958
  title: pY-STAT3 dimer translocates to the nucleus downstream of ALK mutants
  findings: []
- id: file:human/KIF5B/KIF5B-uniprot.txt
  title: UniProt record for human KIF5B
  findings: []
- id: file:human/KIF5B/KIF5B-notes.md
  title: KIF5B curation notes
  findings: []
- id: file:projects/PROTEOSTASIS/mappings/autophagy_lysosome_pathway.yaml
  title: Proteostasis Network autophagy-lysosome pathway mappings
  findings: []
core_functions:
- description: ATP-dependent plus-end-directed microtubule motor activity as the 
    kinesin-1 heavy chain, driving transport of vesicles and organelles along 
    microtubules. Cargo specificity is supplied by adaptor proteins and light-chain 
    interactions, so cargo-specific phenotypes should be interpreted as contexts of this
    core motor function.
  molecular_function:
    id: GO:0008574
    label: plus-end-directed microtubule motor activity
  directly_involved_in:
  - id: GO:0047496
    label: vesicle transport along microtubule
  locations:
  - id: GO:0005856
    label: cytoskeleton
  supported_by:
  - reference_id: PMID:8606779
    supporting_text: Kinesin is the founding member of a superfamily of microtubule 
      based motor proteins that perform force-generating tasks such as organelle 
      transport and chromosome segregation.
  - reference_id: file:human/KIF5B/KIF5B-uniprot.txt
    supporting_text: Microtubule-dependent motor required for normal distribution of 
      mitochondria and lysosomes.
  - reference_id: PMID:24088571
    supporting_text: Kinesin-1 regulates long-range anterograde intracellular transport 
      of vesicles, including lysosomes
proposed_new_terms: []
suggested_questions:
- question: Does direct starvation-recovery or autophagic lysosome reformation evidence 
    support annotating KIF5B to a lysosome-organization/ALR term, or should KIF5B remain
    annotated to lysosome localization only?
  experts:
  - Sean Munro
  - Cleo T. Schiefermeier
- question: Should GO curation distinguish kinesin-1 heavy-chain core motor activity 
    from cargo-specific downstream outcomes such as NK cytotoxicity, channel 
    trafficking, and neuronal synaptic phenotypes?
  experts:
  - GO curator
  - kinesin-1 domain expert
suggested_experiments:
- description: Measure KIF5B recruitment to autolysosomes and lysosomal tubules during 
    starvation and nutrient refeeding, using endogenous tagging plus KIF5B 
    depletion/rescue.
  experiment_type: live-cell imaging/genetic rescue
  hypothesis: KIF5B contributes to lysosome positioning during recovery but requires 
    additional evidence before annotation to autophagic lysosome reformation or lysosome
    organization.
- description: Compare wild-type KIF5B and motor-dead or cargo-adaptor-binding mutants 
    for lysosome localization, lytic granule polarization, and generic vesicle transport
    in matched cell systems.
  experiment_type: structure-function perturbation
  hypothesis: Cargo-specific KIF5B phenotypes depend on the same core plus-end-directed 
    motor activity but separable adaptor interactions.