KRT19 encodes keratin 19 (K19, CK19), the smallest type I (acidic) keratin and the only intermediate filament protein lacking a C-terminal tail domain. As an obligate heteropolymer partner of type II keratins, chiefly KRT8 in simple epithelia, it forms parallel coiled-coil heterodimers that assemble into cytoplasmic keratin intermediate filaments, providing mechanical resilience to simple and ductal epithelia (biliary, gastrointestinal, mammary, urothelial) and to basal cells of the hair follicle outer root sheath and fetal epidermis. In striated muscle, K19-containing filaments concentrate at costameres, bind the actin-binding domain of dystrophin and link the sarcolemma to the contractile apparatus; K19-null mice develop a mild myopathy with costamere disruption. KRT19 is a widely used marker of cholangiocytes, epithelial progenitors and carcinoma cells, and has been reported to act as a signalling scaffold (Src/TGM2, beta-catenin/RAC1, cyclin D3) in cancer cell lines.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0002009 morphogenesis of an epithelium | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: IBA propagation of morphogenesis of an epithelium across the type I keratin family node (PTN002757528). Reason: Keratin filaments contribute to epithelial mechanical integrity, which is a structural contribution to epithelial tissue morphogenesis at the family level. There is no KRT19-specific evidence of an epithelial morphogenesis defect, so this is retained as a non-core, family-level inference rather than a core function. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0005198 structural molecule activity | IEA GO_REF:0000002 | ACCEPT | Summary: InterPro2GO mapping (Keratin type I, IPR002957) to structural molecule activity. Reason: Correct but general; KRT19 is a structural IF protein. The more specific GO:0005200 structural constituent of cytoskeleton is also annotated. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0005200 structural constituent of cytoskeleton | TAS PMID:2448790 Sequence of the human 40-kDa keratin reveals an unusual stru... | ACCEPT | Summary: KRT19 is a structural subunit of keratin intermediate filaments (traceable author statement from the sequence paper). Reason: This is the core molecular function of KRT19: a type I keratin that copolymerizes with type II keratins (principally KRT8) into keratin IFs that provide mechanical integrity to epithelial cells. Supporting Evidence: PMID:2448790 This in turn suggests an important and universal role for this intermediate filament subunit in all species. file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0005515 protein binding | IPI PMID:15846844 Proteomic profiling of cellular proteins interacting with th... | REMOVE | Summary: Pull-down/proteomics study identifying cytokeratin 19 among hepatocyte cytoplasmic proteins bound to immobilized hepatitis C virus core protein. Reason: Generic protein binding to a viral protein (HCV core) is uninformative about KRT19 function; the follow-up validation in this study focused on cytokeratin 8 and vimentin rather than K19. Removal does not dispute that the interaction was detected. No more informative host molecular function is supported. Supporting Evidence: PMID:15846844 nine protein spots were identified as intermediate microfilament proteins, including cytokeratins (five spots for cytokeratin 8, two for cytokeratin 19, and one for cytokeratin 18) and vimentin |
| GO:0005515 protein binding | IPI PMID:16000376 Specific interaction of the actin-binding domain of dystroph... | REMOVE | Summary: Dystrophin actin-binding domain (Dys-ABD) binds K19 directly and specifically in COS-7 cells and in vitro. Reason: The interaction is real and important, but generic protein binding (GO:0005515) is uninformative. Its functional meaning - K19 filaments linking the costamere/dystrophin complex to the contractile apparatus - is captured by the existing costamere (GO:0043034) and structural constituent of muscle (GO:0008307) annotations from the same paper. There is no specific 'dystrophin binding' MF term in GO. Supporting Evidence: PMID:16000376 Studies in COS-7 cells and in vitro showed that Dys-ABD binds directly and specifically to K19. |
| GO:0005515 protein binding | IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and O14964. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:16189514 yeast two-hybrid system, we tested pairwise interactions among the products of |
| GO:0005515 protein binding | IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in... | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT6B (P04259). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT6B binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:16189514 yeast two-hybrid system, we tested pairwise interactions among the products of |
| GO:0005515 protein binding | IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q8IYI6. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:16189514 yeast two-hybrid system, we tested pairwise interactions among the products of |
| GO:0005515 protein binding | IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9NYB9. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:16189514 yeast two-hybrid system, we tested pairwise interactions among the products of |
| GO:0005515 protein binding | IPI PMID:20080707 EGF potentiated oncogenesis requires a tissue transglutamina... | MODIFY | Summary: K19 identified as a tissue transglutaminase (TGM2/tTG) binding partner in SKBR3 breast cancer cells, required for tTG association with Src and enhanced Src activity. Reason: The paper reports that K19 bridges tTG and c-Src, i.e. a bridging (adaptor) role rather than mere binding. GO:0030674 protein-macromolecule adaptor activity captures this better than protein binding. This is a single cancer-cell-line study and is non-core to KRT19's structural IF role. Proposed replacements: protein-macromolecule adaptor activity Supporting Evidence: PMID:20080707 we identified the intermediate filament K19 as a tTG-binding partner PMID:20080707 Our findings that K19 serves to bridge the interaction between tTG and Src |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and O15160. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and O60573. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and P0C1Z6-2. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT2 (P35908). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT2 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q6NYC8. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q7KZS0. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q8IYE0-2. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q8IYX8-2. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q96CS2. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9BVG8. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9H257. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9H5Z6. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9NRA8. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9NX04. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9P0N9. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9UKT9. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9Y2J4-4. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25416956 binary protein-protein interactions. At equal quality, this map is |
| GO:0005515 protein binding | IPI PMID:25910212 Widespread macromolecular interaction perturbations in human... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and P14136. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:25910212 interactions, two-thirds of disease-associated alleles perturb protein-protein |
| GO:0005515 protein binding | IPI PMID:25910212 Widespread macromolecular interaction perturbations in human... | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT2 (P35908). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT2 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:25910212 interactions, two-thirds of disease-associated alleles perturb protein-protein |
| GO:0005515 protein binding | IPI PMID:26871637 Widespread Expansion of Protein Interaction Capabilities by ... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9BT78. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:26871637 of binary protein-protein interactions (PPIs) and identified widespread interaction |
| GO:0005515 protein binding | IPI PMID:29892012 An interactome perturbation framework prioritizes damaging m... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9NYB9. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:29892012 Here we establish an experimentally and computationally integrated approach to investigate the functional impact of missense mutations in the context of the human interactome network |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT2 (P35908). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT2 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:31515488 interactions, generating interaction profiles for 4797 SNV-interaction pairs, of |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9H257. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:31515488 interactions, generating interaction profiles for 4797 SNV-interaction pairs, of |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9P0N9. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:31515488 interactions, generating interaction profiles for 4797 SNV-interaction pairs, of |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and A2BDD9. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and O14964. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT86 (O43790). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT86 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and O95295. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT75 (O95678). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT75 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT6A (P02538). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT6A binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT6B (P04259). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT6B binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT1 (P04264). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT1 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT8 (P05787). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT8 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT3 (P12035). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT3 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT5 (P13647). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT5 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and P14136. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT4 (P19013). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT4 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and P25786. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT2 (P35908). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT2 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and P40617. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and P41219. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT6C (P48668). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT6C binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT83 (P78385). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT83 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT85 (P78386). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT85 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT81 (Q14533). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT81 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT72 (Q14CN4). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT72 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q16512. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT71 (Q3SY84). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT71 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT79 (Q5XKE5). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT79 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT80 (Q6KB66-2). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT80 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT74 (Q7RTS7). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT74 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT77 (Q7Z794). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT77 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q8IVS8. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q8IYI6. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MODIFY | Summary: High-throughput binary interaction (yeast two-hybrid based) between KRT19 and the type II keratin KRT78 (Q8N1N4). Reason: Type I keratins such as KRT19 obligatorily heterodimerize with type II keratins via parallel coiled-coil rod domains; a KRT78 binary hit is the expected keratin type I/type II pairing. Generic protein binding is uninformative; GO:0046982 protein heterodimerization activity is the informative MF. (Many of these partners are not co-expressed with KRT19 in vivo, but the activity being described - type I/type II heterodimerization - is genuine.) Proposed replacements: protein heterodimerization activity Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q8NI38. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q8TCE9. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q8TD31-3. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q969G3. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q96BZ8. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q96ES7. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9BT92. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9BXY8. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9NX04. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9NYB9-2. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9NZD8. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9P0N9. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9P2K3-2. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9UGP5-2. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9Y5F1. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32296183 reference interactome map of human binary protein interactions, or 'HuRI' |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and O43464. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32814053 candidate interactions and is generated by systematic yeast two-hybrid |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and O76024. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32814053 candidate interactions and is generated by systematic yeast two-hybrid |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and P01112. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32814053 candidate interactions and is generated by systematic yeast two-hybrid |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and P05129. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32814053 candidate interactions and is generated by systematic yeast two-hybrid |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and P06396. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32814053 candidate interactions and is generated by systematic yeast two-hybrid |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and P07196. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32814053 candidate interactions and is generated by systematic yeast two-hybrid |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and P09172. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32814053 candidate interactions and is generated by systematic yeast two-hybrid |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and P14136. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32814053 candidate interactions and is generated by systematic yeast two-hybrid |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and P22607. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32814053 candidate interactions and is generated by systematic yeast two-hybrid |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and P28329-3. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32814053 candidate interactions and is generated by systematic yeast two-hybrid |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and P41219. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32814053 candidate interactions and is generated by systematic yeast two-hybrid |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and P42858. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32814053 candidate interactions and is generated by systematic yeast two-hybrid |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q8WXH2. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32814053 candidate interactions and is generated by systematic yeast two-hybrid |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q92876. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32814053 candidate interactions and is generated by systematic yeast two-hybrid |
| GO:0005515 protein binding | IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ... | REMOVE | Summary: High-throughput binary protein interaction screen hit between KRT19 and Q9Y5Z0. Reason: Generic protein binding from a high-throughput interactome screen; the partner is not a type II keratin and there is no independent evidence that this interaction underlies a KRT19 function. Keratin rod domains are sticky coiled coils that yield many Y2H hits. Removal reflects lack of functional information, not that the interaction is false. Supporting Evidence: PMID:32814053 candidate interactions and is generated by systematic yeast two-hybrid |
| GO:0005829 cytosol | IEA GO_REF:0000117 | ACCEPT | Summary: ARBA machine-learning prediction of cytosol localization. Reason: Keratin filaments are cytoplasmic and the soluble keratin pool is cytosolic; cytosol is an acceptable, if general, location. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6805546 | ACCEPT | Summary: Reactome keratin filament assembly reaction (Reactome:R-HSA-6805546) placing KRT19 in the cytosol. Reason: Reactome models keratin type I/type II heterodimerization and filament assembly in the cytosol; KRT19 participates in these steps as a type I keratin. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6805573 | ACCEPT | Summary: Reactome keratin filament assembly reaction (Reactome:R-HSA-6805573) placing KRT19 in the cytosol. Reason: Reactome models keratin type I/type II heterodimerization and filament assembly in the cytosol; KRT19 participates in these steps as a type I keratin. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6806610 | ACCEPT | Summary: Reactome keratin filament assembly reaction (Reactome:R-HSA-6806610) placing KRT19 in the cytosol. Reason: Reactome models keratin type I/type II heterodimerization and filament assembly in the cytosol; KRT19 participates in these steps as a type I keratin. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6806613 | ACCEPT | Summary: Reactome keratin filament assembly reaction (Reactome:R-HSA-6806613) placing KRT19 in the cytosol. Reason: Reactome models keratin type I/type II heterodimerization and filament assembly in the cytosol; KRT19 participates in these steps as a type I keratin. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6806629 | ACCEPT | Summary: Reactome cornification/epidermal keratin reaction (Reactome:R-HSA-6806629) placing the generic keratin filament set, including KRT19, in the cytosol. Reason: The cytosolic location of keratin filaments is correct for KRT19, so the location is accepted. Note that the reaction context (hair keratin fibres, cornified envelope, filaggrin) is specific to stratified epidermis/hair and reflects inclusion of all keratins in a generic Reactome entity set rather than KRT19-specific biology. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6809393 | ACCEPT | Summary: Reactome keratin filament assembly reaction (Reactome:R-HSA-6809393) placing KRT19 in the cytosol. Reason: Reactome models keratin type I/type II heterodimerization and filament assembly in the cytosol; KRT19 participates in these steps as a type I keratin. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6809663 | ACCEPT | Summary: Reactome cornification/epidermal keratin reaction (Reactome:R-HSA-6809663) placing the generic keratin filament set, including KRT19, in the cytosol. Reason: The cytosolic location of keratin filaments is correct for KRT19, so the location is accepted. Note that the reaction context (hair keratin fibres, cornified envelope, filaggrin) is specific to stratified epidermis/hair and reflects inclusion of all keratins in a generic Reactome entity set rather than KRT19-specific biology. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6810357 | ACCEPT | Summary: Reactome cornification/epidermal keratin reaction (Reactome:R-HSA-6810357) placing the generic keratin filament set, including KRT19, in the cytosol. Reason: The cytosolic location of keratin filaments is correct for KRT19, so the location is accepted. Note that the reaction context (hair keratin fibres, cornified envelope, filaggrin) is specific to stratified epidermis/hair and reflects inclusion of all keratins in a generic Reactome entity set rather than KRT19-specific biology. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6810937 | ACCEPT | Summary: Reactome cornification/epidermal keratin reaction (Reactome:R-HSA-6810937) placing the generic keratin filament set, including KRT19, in the cytosol. Reason: The cytosolic location of keratin filaments is correct for KRT19, so the location is accepted. Note that the reaction context (hair keratin fibres, cornified envelope, filaggrin) is specific to stratified epidermis/hair and reflects inclusion of all keratins in a generic Reactome entity set rather than KRT19-specific biology. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6811539 | ACCEPT | Summary: Reactome cornification/epidermal keratin reaction (Reactome:R-HSA-6811539) placing the generic keratin filament set, including KRT19, in the cytosol. Reason: The cytosolic location of keratin filaments is correct for KRT19, so the location is accepted. Note that the reaction context (hair keratin fibres, cornified envelope, filaggrin) is specific to stratified epidermis/hair and reflects inclusion of all keratins in a generic Reactome entity set rather than KRT19-specific biology. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6814298 | ACCEPT | Summary: Reactome cornification/epidermal keratin reaction (Reactome:R-HSA-6814298) placing the generic keratin filament set, including KRT19, in the cytosol. Reason: The cytosolic location of keratin filaments is correct for KRT19, so the location is accepted. Note that the reaction context (hair keratin fibres, cornified envelope, filaggrin) is specific to stratified epidermis/hair and reflects inclusion of all keratins in a generic Reactome entity set rather than KRT19-specific biology. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6814387 | ACCEPT | Summary: Reactome cornification/epidermal keratin reaction (Reactome:R-HSA-6814387) placing the generic keratin filament set, including KRT19, in the cytosol. Reason: The cytosolic location of keratin filaments is correct for KRT19, so the location is accepted. Note that the reaction context (hair keratin fibres, cornified envelope, filaggrin) is specific to stratified epidermis/hair and reflects inclusion of all keratins in a generic Reactome entity set rather than KRT19-specific biology. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6814734 | ACCEPT | Summary: Reactome cornification/epidermal keratin reaction (Reactome:R-HSA-6814734) placing the generic keratin filament set, including KRT19, in the cytosol. Reason: The cytosolic location of keratin filaments is correct for KRT19, so the location is accepted. Note that the reaction context (hair keratin fibres, cornified envelope, filaggrin) is specific to stratified epidermis/hair and reflects inclusion of all keratins in a generic Reactome entity set rather than KRT19-specific biology. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6814764 | ACCEPT | Summary: Reactome cornification/epidermal keratin reaction (Reactome:R-HSA-6814764) placing the generic keratin filament set, including KRT19, in the cytosol. Reason: The cytosolic location of keratin filaments is correct for KRT19, so the location is accepted. Note that the reaction context (hair keratin fibres, cornified envelope, filaggrin) is specific to stratified epidermis/hair and reflects inclusion of all keratins in a generic Reactome entity set rather than KRT19-specific biology. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8942224 | ACCEPT | Summary: Reactome cornification/epidermal keratin reaction (Reactome:R-HSA-8942224) placing the generic keratin filament set, including KRT19, in the cytosol. Reason: The cytosolic location of keratin filaments is correct for KRT19, so the location is accepted. Note that the reaction context (hair keratin fibres, cornified envelope, filaggrin) is specific to stratified epidermis/hair and reflects inclusion of all keratins in a generic Reactome entity set rather than KRT19-specific biology. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0005856 cytoskeleton | IBA GO_REF:0000033 | ACCEPT | Summary: IBA: cytoskeleton. Reason: Correct general location; KRT19 is a component of the keratin IF cytoskeleton. More specific terms (keratin filament, IF cytoskeleton) are also annotated. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0005882 intermediate filament | TAS PMID:2448790 Sequence of the human 40-kDa keratin reveals an unusual stru... | ACCEPT | Summary: KRT19 is an intermediate filament protein (traceable author statement). Reason: KRT19 is a type I keratin IF subunit; intermediate filament is a correct core location (keratin filament GO:0045095 being the more specific child). Supporting Evidence: PMID:2448790 This in turn suggests an important and universal role for this intermediate filament subunit in all species. |
| GO:0005886 plasma membrane | IDA PMID:23377137 Expression of caspase-14 and keratin-19 in the human epiderm... | MARK AS OVER ANNOTATED | Summary: Immunohistochemistry of KRT19 in human fetal epidermis and appendages; annotated to plasma membrane. Reason: KRT19 is a cytoplasmic IF protein, not a plasma-membrane protein. Peripheral/membrane-proximal immunostaining reflects filaments anchored at desmosomes or the cell cortex. The abstract (full text not available here) describes KRT19 as a marker of germinative layers and gives no membrane-specific finding. This is not removed, because the curator saw the full text, but plasma membrane overstates the localization; cell periphery (GO:0071944, already annotated) is the appropriate level. Supporting Evidence: PMID:23377137 keratin-19 marked the germinative epithelial layers in the fetal epidermis and appendages and possibly the nests of epidermal stem cells |
| GO:0008307 structural constituent of muscle | IDA PMID:16000376 Specific interaction of the actin-binding domain of dystroph... | ACCEPT | Summary: K19 concentrates at costameres in striated muscle and binds the dystrophin ABD directly; expression in muscle fibres alters sarcolemmal organization. Reason: Well-supported structural role in striated muscle, reinforced by the K19-null mouse myopathy with costamere disruption (PMID:17971417). It is a tissue-specific, secondary role relative to KRT19's main function in epithelial IFs, accepted as a tissue-specific core function. Supporting Evidence: PMID:16000376 Cytokeratins 8 and 19 concentrate at costameres of striated muscle and copurify with the dystrophin-glycoprotein complex PMID:17971417 Our results suggest that keratin 19 in fast-twitch skeletal muscle helps organize costameres and links them to the contractile apparatus |
| GO:0008307 structural constituent of muscle | IEA GO_REF:0000107 | ACCEPT | Summary: Ensembl Compara transfer of structural constituent of muscle from mouse Krt19. Reason: Consistent with the human IDA (PMID:16000376) and mouse knockout data (PMID:17971417); accepted as the tissue-specific muscle structural role. Supporting Evidence: PMID:17971417 Our results suggest that keratin 19 in fast-twitch skeletal muscle helps organize costameres and links them to the contractile apparatus |
| GO:0016010 dystrophin-associated glycoprotein complex | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Ensembl Compara transfer of dystrophin-associated glycoprotein complex from mouse. Reason: K19 co-purifies with the dystrophin-glycoprotein complex and binds the dystrophin ABD, but it is an associated IF partner rather than a subunit of the DGC. Costamere (GO:0043034) better represents the localization. Supporting Evidence: PMID:16000376 Cytokeratins 8 and 19 concentrate at costameres of striated muscle and copurify with the dystrophin-glycoprotein complex PMID:17971417 The costameric dystrophin-dystroglycan complex, which co-purified with gamma-actin, keratin 8 and keratin 19 from striated muscles of wild-type mice |
| GO:0016327 apicolateral plasma membrane | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Ensembl Compara transfer of apicolateral plasma membrane from mouse. Reason: KRT19 is not a membrane protein; keratin filaments are concentrated subapically/at the cell cortex in polarized epithelia. A plasma membrane subcompartment overstates the localization. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0030018 Z disc | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Ensembl Compara transfer of Z disc from mouse. Reason: In striated muscle keratin filaments containing K19 link costameres (which align with Z discs) to the contractile apparatus; plausible, non-core muscle-specific localization. Supporting Evidence: PMID:21209367 Intermediate filaments, composed of desmin and of keratins, play important roles in linking contractile elements to each other and to the sarcolemma in striated muscle. file:human/KRT19/KRT19-uniprot.txt Specifically interacts with the actin-binding domain of dystrophin, helping to link the contractile apparatus to dystrophin at the costameres of striated muscle |
| GO:0030280 structural constituent of skin epidermis | IBA GO_REF:0000033 | MARK AS OVER ANNOTATED | Summary: IBA: structural constituent of skin epidermis propagated across the type I keratin node. Reason: KRT19 is a simple/ductal epithelial keratin. In the interfollicular epidermis it is restricted to fetal germinative layers and rare basal cell nests, declining with epidermal maturation, so it does not make a significant contribution to the structural integrity of the epidermis (unlike KRT10/KRT14). This family-level propagation over-reaches for KRT19; KRT19 also lacks the tail domain of epidermal keratins. Propagation Review Root cause: PROPAGATION BAD Failure modes: CONTEXT OR TISSUE MISMATCH Sources checked: PANTHER:PTN002757528 SUPPORTS SOURCE BUT NOT TARGET Type I keratin node seeded by epidermal keratins (e.g. KRT10 P13645); the epidermal structural role does not hold for the simple-epithelial, tailless KRT19. Supporting Evidence: PMID:23377137 keratin-19 expression gradually decreased with epidermal maturation through gestation PMID:2448790 The DNA sequence encodes a protein of 44,098 Da, which is unique in that it lacks the terminal non-alpha-helical tail segment found in all other keratins |
| GO:0030855 epithelial cell differentiation | IBA GO_REF:0000033 | MARK AS OVER ANNOTATED | Summary: IBA: epithelial cell differentiation propagated across a keratin family node. Reason: KRT19 is widely used as a marker of epithelial lineages (e.g. ductal plate cholangiocyte precursors, germinative epidermal layers), but being a marker is not participation. There is no evidence that KRT19 performs a step in epithelial cell differentiation. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: ROLE CONFLATION Sources checked: PANTHER:PTN008383948 SUPPORTS SOURCE BUT NOT TARGET Keratin node whose donors include phenotypes of differentiation-associated keratins; for KRT19 the evidence is marker expression, not participation in differentiation. Supporting Evidence: PMID:19185580 a transient sheet of CK19-expressing hepatoblasts that gives rise to mature bile ducts file:human/KRT19/KRT19-deep-research-falcon.md a CK19-positive stain does not by itself show that K19 caused the observed differentiation |
| GO:0042383 sarcolemma | IEA GO_REF:0000107 | ACCEPT | Summary: Ensembl Compara transfer of sarcolemma from mouse. Reason: K19 associates with dystrophin at the sarcolemma of striated muscle; supported by human and mouse data. Accepted as the location of the muscle structural role. Supporting Evidence: PMID:16000376 Our results show that dystrophin interacts through its ABD with K19 specifically and are consistent with the idea that cytokeratins associate with dystrophin at the sarcolemma of striated muscle. |
| GO:0043034 costamere | IDA PMID:16000376 Specific interaction of the actin-binding domain of dystroph... | ACCEPT | Summary: K8 and K19 concentrate at costameres of striated muscle. Reason: Direct evidence for costameric localization, supported by K19-null mouse costamere disruption. Accepted as the location of KRT19's tissue-specific structural role in striated muscle. Supporting Evidence: PMID:16000376 Cytokeratins 8 and 19 concentrate at costameres of striated muscle and copurify with the dystrophin-glycoprotein complex PMID:17971417 Costameres at the sarcolemma of keratin-19-null muscle, visualized with antibodies against spectrin or dystrophin, were disrupted |
| GO:0043627 response to estrogen | IEP PMID:10037815 Combining SSH and cDNA microarrays for rapid identification ... | MARK AS OVER ANNOTATED | Summary: KRT19 was among transcripts over-expressed in ER-positive vs ER-negative breast cancer cell lines in an SSH/microarray screen. Reason: The evidence is differential expression between ER-positive and ER-negative cell lines, not a change in KRT19 expression in response to estrogen treatment. Correlation with ER status does not establish a response to estrogen, and even an expression response would not reflect KRT19 function. Supporting Evidence: PMID:10037815 Ten clones were identified that were over-expressed by at least a factor of five in the ER-positive cell lines. |
| GO:0044877 protein-containing complex binding | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Ensembl Compara transfer of protein-containing complex binding from mouse (likely DGC/costamere association). Reason: Vague binding term; the underlying observation (association with the dystrophin-glycoprotein complex via the dystrophin ABD) is better represented by costamere and structural constituent of muscle. Not wrong but adds little. Supporting Evidence: PMID:16000376 Cytokeratins 8 and 19 concentrate at costameres of striated muscle and copurify with the dystrophin-glycoprotein complex |
| GO:0045095 keratin filament | IBA GO_REF:0000033 | ACCEPT | Summary: IBA: keratin filament. Reason: Core location. KRT19 is a type I keratin forming keratin filaments with type II keratins (KRT8 in simple epithelia). Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells file:human/KRT19/KRT19-uniprot.txt Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers |
| GO:0045109 intermediate filament organization | IBA GO_REF:0000033 | ACCEPT | Summary: IBA: intermediate filament organization. Reason: As an obligate IF subunit, KRT19 directly participates in assembly and organization of the keratin IF network (heterodimer to tetramer to filament). Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Heterodimers associate in an antiparallel manner to form heterotetramers, which further assemble into higher-order keratin intermediate filaments |
| GO:0045111 intermediate filament cytoskeleton | IDA GO_REF:0000052 | ACCEPT | Summary: Human Protein Atlas immunofluorescence localizes KRT19 to the intermediate filament cytoskeleton. Reason: Core localization consistent with its role as a keratin IF subunit. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
| GO:0045214 sarcomere organization | IDA PMID:16000376 Specific interaction of the actin-binding domain of dystroph... | KEEP AS NON CORE | Summary: Expression of K19 in muscle fibres in vivo disrupted organization of the sarcolemma; K19 links costameres to the contractile apparatus. Reason: The cited study shows K19 overexpression disrupts sarcolemmal organization (K8, not K19, incorporated into sarcomeres); knockout data show costamere disruption and subsarcolemmal reorganization. The curator's sarcomere organization call may reflect full-text details not visible here; retained as non-core muscle-specific process, noting that costamere/sarcolemma organization is the better-supported aspect. Supporting Evidence: PMID:16000376 Expressed in muscle fibers in vivo, K19 accumulated in the myoplasm in structures that contained dystrophin and spectrin and disrupted the organization of the sarcolemma. PMID:17971417 Our results suggest that keratin 19 in fast-twitch skeletal muscle helps organize costameres and links them to the contractile apparatus |
| GO:0070062 extracellular exosome | HDA PMID:19199708 Proteomic analysis of human parotid gland exosomes by multid... | KEEP AS NON CORE | Summary: Identified by MudPIT proteomics in human parotid saliva exosomes. Reason: High-throughput proteomic detection; keratins are common in exosome preparations. Not informative about function. Supporting Evidence: PMID:19199708 we catalogued 491 proteins in the exosome fraction of human parotid saliva |
| GO:0071944 cell periphery | IDA PMID:19185580 The microRNA-30 family is required for vertebrate hepatobili... | KEEP AS NON CORE | Summary: CK19 immunostaining in ductal plate cells in a study of miR-30 in hepatobiliary development. Reason: CK19 was used as a ductal-plate marker; peripheral filament staining is consistent with cortical keratin networks. Acceptable general location, non-core. Supporting Evidence: PMID:19185580 miR-30a was expressed in CK19-positive ductal plate cells |
| GO:1990357 terminal web | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Ensembl Compara transfer of terminal web from mouse. Reason: In intestinal and other polarized simple epithelia, keratin filaments (K8/K18/K19/K20) are concentrated in the apical terminal web region; plausible non-core location transferred from the mouse ortholog. Supporting Evidence: file:human/KRT19/KRT19-uniprot.txt Structural component of intermediate filaments in epithelial cells. Forms intermediate filaments by heterodimerizing with the type II keratin KRT8, providing mechanical integrity to epithelial cells |
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Download this section (compressed HTML)Q: What does the tailless KRT19 rod contribute that KRT18 does not, given that both pair with KRT8 in simple epithelia and single K19-null mice show little epithelial phenotype?
Q: Are the reported scaffolding interactions of K19 (TGM2-Src, beta-catenin/RAC1, cyclin D3) properties of filamentous K19 or of a soluble pool, and do they occur in non-transformed epithelia?
Q: Should GO represent the selective binding of K19 to the dystrophin actin-binding domain with a more specific molecular function than protein binding (no 'dystrophin binding' term currently exists)?
Experiment: Compare keratin network integrity, cell fragility and injury susceptibility in biliary organoids from Krt19-null versus wild-type mice, and in human cholangiocyte organoids with KRT19 knockout, under stretch and bile-acid challenge.
Hypothesis: K19 is required for keratin network resilience in cholangiocytes under mechanical or toxic stress.
Type: genetic perturbation
Experiment: Express assembly-incompetent K19 rod mutants versus wild type in KRT19-knockout MCF7 cells and test binding to beta-catenin, cyclin D3 and TGM2 by proximity labelling and co-immunoprecipitation.
Hypothesis: Filamentous rather than soluble K19 mediates signalling-scaffold interactions reported in cancer cells.
Type: biochemical / cell biology
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