LAMP3

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0005765 lysosomal membrane	EXP PMID:21930964 Reciprocal polarization of T and B cells at the immunologica...	ACCEPT	Summary: LAMP3 is a lysosomal membrane glycoprotein in dendritic cells and other cell types. Reason: Confocal microscopy shows lysosomal distribution in dendritic cells; LAMP3 is a type I membrane protein of the LAMP family with a lysosomal targeting tail. Supporting Evidence: PMID:9768752 Confocal microscopy confirmed the lysosomal distribution of the protein. PMID:9768752 DC-LAMP was found in the MHC class II compartment immediately before the translocation of MHC class II molecules to the cell surface, after which it concentrates into perinuclear lysosomes.
GO:0005765 lysosomal membrane	EXP PMID:9768752 A novel lysosome-associated membrane glycoprotein, DC-LAMP, ...	ACCEPT	Summary: LAMP3 is a lysosomal membrane glycoprotein in dendritic cells and other cell types. Reason: Confocal microscopy shows lysosomal distribution in dendritic cells; LAMP3 is a type I membrane protein of the LAMP family with a lysosomal targeting tail. Supporting Evidence: PMID:9768752 Confocal microscopy confirmed the lysosomal distribution of the protein. PMID:9768752 DC-LAMP was found in the MHC class II compartment immediately before the translocation of MHC class II molecules to the cell surface, after which it concentrates into perinuclear lysosomes.
GO:0005765 lysosomal membrane	IBA GO_REF:0000033	ACCEPT	Summary: LAMP3 is a lysosomal membrane glycoprotein in dendritic cells and other cell types. Reason: Confocal microscopy shows lysosomal distribution in dendritic cells; LAMP3 is a type I membrane protein of the LAMP family with a lysosomal targeting tail. Supporting Evidence: PMID:9768752 Confocal microscopy confirmed the lysosomal distribution of the protein. PMID:9768752 DC-LAMP was found in the MHC class II compartment immediately before the translocation of MHC class II molecules to the cell surface, after which it concentrates into perinuclear lysosomes.
GO:0005765 lysosomal membrane	IEA GO_REF:0000044	ACCEPT	Summary: LAMP3 is a lysosomal membrane glycoprotein in dendritic cells and other cell types. Reason: Confocal microscopy shows lysosomal distribution in dendritic cells; LAMP3 is a type I membrane protein of the LAMP family with a lysosomal targeting tail. Supporting Evidence: PMID:9768752 Confocal microscopy confirmed the lysosomal distribution of the protein. PMID:9768752 DC-LAMP was found in the MHC class II compartment immediately before the translocation of MHC class II molecules to the cell surface, after which it concentrates into perinuclear lysosomes.
GO:0005765 lysosomal membrane	TAS PMID:9721848 Isolation and characterization of a novel human lung-specifi...	ACCEPT	Summary: LAMP3 is a lysosomal membrane glycoprotein in dendritic cells and other cell types. Reason: Confocal microscopy shows lysosomal distribution in dendritic cells; LAMP3 is a type I membrane protein of the LAMP family with a lysosomal targeting tail. Supporting Evidence: PMID:9768752 Confocal microscopy confirmed the lysosomal distribution of the protein. PMID:9768752 DC-LAMP was found in the MHC class II compartment immediately before the translocation of MHC class II molecules to the cell surface, after which it concentrates into perinuclear lysosomes.
GO:0005769 early endosome	IDA PMID:21810281 Lysosome-associated membrane glycoprotein 3 is involved in i...	MODIFY	Summary: LAMP3-positive vesicles colocalize with incoming influenza NP early in infection. Reason: LAMP3 is a membrane protein, so the early endosome membrane term already carried is the precise form; the endosomal localization is retained as non-core. Proposed replacements: early endosome membrane Supporting Evidence: PMID:21810281 LAMP3 is an influenza A virus inducible gene, and plays an important role in viral post-entry steps.
GO:0005886 plasma membrane	IBA GO_REF:0000033	KEEP AS NON CORE	Summary: A pool of LAMP3 is present at the cell surface. Reason: LAMP3 was observed at the surface of THP-1 macrophages before relocalizing to Salmonella vacuoles, and LAMP proteins cycle via the plasma membrane; the surface pool is minor relative to lysosomes, with the glycosylated luminal domain facing outward. Supporting Evidence: PMID:27329040 LAMP-3, like LAMP-2, shifts its localization from the cell surface to alongside Salmonella.
GO:0005886 plasma membrane	IDA PMID:21810281 Lysosome-associated membrane glycoprotein 3 is involved in i...	KEEP AS NON CORE	Summary: A pool of LAMP3 is present at the cell surface. Reason: LAMP3 was observed at the surface of THP-1 macrophages before relocalizing to Salmonella vacuoles, and LAMP proteins cycle via the plasma membrane; the surface pool is minor relative to lysosomes, with the glycosylated luminal domain facing outward. Supporting Evidence: PMID:27329040 LAMP-3, like LAMP-2, shifts its localization from the cell surface to alongside Salmonella.
GO:0009986 cell surface	EXP PMID:27329040 LAMP-3 (Lysosome-Associated Membrane Protein 3) Promotes the...	KEEP AS NON CORE	Summary: A pool of LAMP3 is present at the cell surface. Reason: LAMP3 was observed at the surface of THP-1 macrophages before relocalizing to Salmonella vacuoles, and LAMP proteins cycle via the plasma membrane; the surface pool is minor relative to lysosomes, with the glycosylated luminal domain facing outward. Supporting Evidence: PMID:27329040 LAMP-3, like LAMP-2, shifts its localization from the cell surface to alongside Salmonella.
GO:0009986 cell surface	IEA GO_REF:0000044	KEEP AS NON CORE	Summary: A pool of LAMP3 is present at the cell surface. Reason: LAMP3 was observed at the surface of THP-1 macrophages before relocalizing to Salmonella vacuoles, and LAMP proteins cycle via the plasma membrane; the surface pool is minor relative to lysosomes, with the glycosylated luminal domain facing outward. Supporting Evidence: PMID:27329040 LAMP-3, like LAMP-2, shifts its localization from the cell surface to alongside Salmonella.
GO:0010506 regulation of autophagy	IMP PMID:25681212 Loss of lysosome-associated membrane protein 3 (LAMP3) enhan...	KEEP AS NON CORE	Summary: LAMP3 induction promotes autophagic flux during proteasome inhibition. Reason: As an ATF4/UPR target LAMP3 supports autophagic flux and survival; the mechanism is undefined. Supporting Evidence: PMID:25681212 Induction of LAMP3 mRNA following proteasomal inhibition was dependent on UPR transcription factor ATF4 signaling and induced autophagic flux.
GO:0010628 positive regulation of gene expression	IMP PMID:23651994 Gene network reconstruction reveals cell cycle and antiviral...	KEEP AS NON CORE	Summary: LAMP3 positively regulates interferon-dependent antiviral gene expression in cervical cancer cells. Reason: The full text shows siRNA knockdown of LAMP3 in HeLa cells reduced interferon-dependent antiviral gene expression (STAT1, IRF7, HERC5, ISG20, OAS1), identifying LAMP3 as a positive regulator of this gene-expression program; this is a genuine but cancer-context, mechanism-undefined role peripheral to LAMP3's core lysosomal/lamellar-body membrane function. Supporting Evidence: PMID:23651994 LAMP3 drives the IFN-dependent gene expression signature
GO:0012505 endomembrane system	IEA GO_REF:0000117	MODIFY	Summary: General membrane assignment. Reason: True but general; LAMP3 is an integral protein of lysosomal membranes, already accepted. Proposed replacements: lysosomal membrane Supporting Evidence: PMID:9768752 Confocal microscopy confirmed the lysosomal distribution of the protein.
GO:0016020 membrane	IEA GO_REF:0000002	MODIFY	Summary: General membrane assignment. Reason: True but general; LAMP3 is an integral protein of lysosomal membranes, already accepted. Proposed replacements: lysosomal membrane Supporting Evidence: PMID:9768752 Confocal microscopy confirmed the lysosomal distribution of the protein.
GO:0030659 cytoplasmic vesicle membrane	EXP PMID:9768752 A novel lysosome-associated membrane glycoprotein, DC-LAMP, ...	KEEP AS NON CORE	Summary: LAMP3 is in cytoplasmic vesicle membranes, including MHC class II compartments. Reason: Supported by dendritic cell localization to MHC II-containing vesicles; the lysosomal and late endosomal membrane terms are more precise for the main pool. Supporting Evidence: PMID:9768752 DC-LAMP was found in the MHC class II compartment immediately before the translocation of MHC class II molecules to the cell surface, after which it concentrates into perinuclear lysosomes.
GO:0030659 cytoplasmic vesicle membrane	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: LAMP3 is in cytoplasmic vesicle membranes, including MHC class II compartments. Reason: Supported by dendritic cell localization to MHC II-containing vesicles; the lysosomal and late endosomal membrane terms are more precise for the main pool. Supporting Evidence: PMID:9768752 DC-LAMP was found in the MHC class II compartment immediately before the translocation of MHC class II molecules to the cell surface, after which it concentrates into perinuclear lysosomes.
GO:0031901 early endosome membrane	EXP PMID:21810281 Lysosome-associated membrane glycoprotein 3 is involved in i...	KEEP AS NON CORE	Summary: LAMP3 detected on early endosomal vesicles in lung epithelial cells. Reason: Curated from imaging in influenza-infected A549 cells; a minor or transit location compared with lysosomes. Supporting Evidence: PMID:21810281 LAMP3 is an influenza A virus inducible gene, and plays an important role in viral post-entry steps.
GO:0031901 early endosome membrane	IEA GO_REF:0000044	KEEP AS NON CORE	Summary: LAMP3 detected on early endosomal vesicles in lung epithelial cells. Reason: Curated from imaging in influenza-infected A549 cells; a minor or transit location compared with lysosomes. Supporting Evidence: PMID:21810281 LAMP3 is an influenza A virus inducible gene, and plays an important role in viral post-entry steps.
GO:0031902 late endosome membrane	IBA GO_REF:0000033	ACCEPT	Summary: LAMP3 is in late endosome/MHC class II compartment membranes. Reason: In dendritic cells LAMP3 occupies the MHC class II compartment, a late endosomal/lysosomal organelle, before reaching perinuclear lysosomes. Supporting Evidence: PMID:9768752 DC-LAMP was found in the MHC class II compartment immediately before the translocation of MHC class II molecules to the cell surface, after which it concentrates into perinuclear lysosomes.
GO:0031982 vesicle	IDA PMID:25681212 Loss of lysosome-associated membrane protein 3 (LAMP3) enhan...	MODIFY	Summary: LAMP3-positive vesicles observed in cells. Reason: LAMP3 is an integral membrane protein of these vesicles; the vesicle membrane term, already carried, is more precise. Proposed replacements: cytoplasmic vesicle membrane Supporting Evidence: PMID:25681212 Induction of LAMP3 mRNA following proteasomal inhibition was dependent on UPR transcription factor ATF4 signaling and induced autophagic flux.
GO:0035455 response to interferon-alpha	IMP PMID:23651994 Gene network reconstruction reveals cell cycle and antiviral...	KEEP AS NON CORE	Summary: LAMP3 participates in the cellular response to type I (alpha) interferon in cervical cancer cells. Reason: The full text stimulated cells with interferon and showed LAMP3 knockdown altered the interferon-dependent antiviral gene signature (which the authors report as a mixed type I and type II IFN pattern); deferring to the curator's term, this GOA row annotates the type I (alpha) component, placing LAMP3 within the cellular response to type I (alpha) interferon. A genuine but non-core, cancer-context role distinct from LAMP3's structural lysosomal membrane function. Supporting Evidence: PMID:23651994 between LAMP3 siRNA and control siRNA treated HeLa cells after interferon stimulation
GO:0046718 symbiont entry into host cell	IMP NOT PMID:21810281 Lysosome-associated membrane glycoprotein 3 is involved in i...	ACCEPT	Summary: Curated NOT annotation; LAMP3 is not involved in symbiont entry into the host cell. Reason: This is a curated NOT (negated) annotation, and the evidence supports the negation. The study places the LAMP3 requirement at viral post-entry steps (NP nuclear accumulation), not entry, so the negation of symbiont entry is correct; keep it as the machine-readable record. LAMP3's actual effect is on later steps of the viral life cycle. Supporting Evidence: PMID:21810281 LAMP3 is an influenza A virus inducible gene, and plays an important role in viral post-entry steps.
GO:0048471 perinuclear region of cytoplasm	IDA PMID:25681212 Loss of lysosome-associated membrane protein 3 (LAMP3) enhan...	KEEP AS NON CORE	Summary: LAMP3 concentrates in perinuclear lysosomes. Reason: Consistent with the perinuclear lysosomal distribution in mature dendritic cells. Supporting Evidence: PMID:9768752 DC-LAMP was found in the MHC class II compartment immediately before the translocation of MHC class II molecules to the cell surface, after which it concentrates into perinuclear lysosomes.
GO:0072594 establishment of protein localization to organelle	IBA GO_REF:0000033	MARK AS OVER ANNOTATED	Summary: Protein localization to organelle propagated from LAMP2 (chaperone-mediated autophagy). Reason: The source activity is LAMP-2A's CMA receptor function, which depends on the LAMP-2A-specific cytosolic tail; there is no evidence that LAMP3 targets proteins to organelles. Propagation Review Root cause: PROPAGATION BAD Failure modes: FUNCTIONAL DIVERGENCE Sources checked: MGI:MGI:96748 SUPPORTS SOURCE BUT NOT TARGET Mouse Lamp2 CMA-related protein targeting; LAMP3 lacks the LAMP-2A cytosolic tail features for substrate binding. Supporting Evidence: PMID:9768752 Confocal microscopy confirmed the lysosomal distribution of the protein.
GO:0097233 alveolar lamellar body membrane	ISS GO_REF:0000024	ACCEPT	Summary: LAMP3 is in the limiting membrane of lamellar bodies of alveolar type II cells (rat data). Reason: LAMP3 is a characteristic marker of type II pneumocytes, where lamellar bodies are lysosome-related organelles; the transfer from rat is consistent with human lung expression of the gene. Supporting Evidence: PMID:9721848 We have isolated and characterized a novel human lung-specific gene
GO:0098588 bounding membrane of organelle	IEA GO_REF:0000117	MODIFY	Summary: General membrane assignment. Reason: True but general; LAMP3 is an integral protein of lysosomal membranes, already accepted. Proposed replacements: lysosomal membrane Supporting Evidence: PMID:9768752 Confocal microscopy confirmed the lysosomal distribution of the protein.
GO:1901799 negative regulation of proteasomal protein catabolic process	IMP PMID:25681212 Loss of lysosome-associated membrane protein 3 (LAMP3) enhan...	UNDECIDED	Summary: Link between LAMP3 and proteasomal degradation. Reason: The cached abstract shows LAMP3 is induced by proteasome inhibition and promotes autophagic flux, not that LAMP3 negatively regulates proteasomal catabolism; the full-text basis for this IMP was not inspected. Supporting Evidence: PMID:25681212 Induction of LAMP3 mRNA following proteasomal inhibition was dependent on UPR transcription factor ATF4 signaling and induced autophagic flux.
GO:1903900 regulation of viral life cycle	IMP PMID:21810281 Lysosome-associated membrane glycoprotein 3 is involved in i...	KEEP AS NON CORE	Summary: LAMP3 supports influenza A virus post-entry replication. Reason: Knockdown reduced viral NP nuclear accumulation and titers; a host-factor role during infection. Supporting Evidence: PMID:21810281 LAMP3 is an influenza A virus inducible gene, and plays an important role in viral post-entry steps.

Gene Description

Existing Annotations Review

Core Functions

Lysosomal/late endosomal membrane glycoprotein of dendritic cells, B cells and alveolar type II cells (lamellar bodies), of undefined molecular activity, that modulates autophagic flux and the endolysosomal compartment during dendritic cell maturation and stress.

References

Suggested Questions for Experts

Suggested Experiments

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