LRRK2

UniProt ID: Q5S007
Organism: Homo sapiens
Review Status: COMPLETE
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Gene Description

LRRK2 (Leucine-rich repeat serine/threonine-protein kinase 2) is a large multi-domain ROCO family protein with dual enzymatic activities - a serine/threonine protein kinase (EC 2.7.11.1) and a GTPase (EC 3.6.5.-). The protein contains ARM/ANK/LRR repeats, ROC (Ras of complex proteins) GTPase domain, COR (C-terminal of ROC) domain, kinase domain, and WD40 repeats. LRRK2 phosphorylates a subset of Rab GTPases (primarily Rab8A, Rab10, Rab12, Rab29) at their switch II regions, regulating endolysosomal trafficking and ciliogenesis. It is predominantly cytosolic but dynamically recruited to Golgi, endosomes, and lysosomes where its Rab substrates reside. Mutations in LRRK2 are the most common genetic cause of familial Parkinson's disease (PARK8).

Existing Annotations Review

GO Term Evidence Action Reason
GO:0035556 intracellular signal transduction
IBA
GO_REF:0000033
ACCEPT
Summary: LRRK2 is a multi-domain ROCO protein kinase that phosphorylates Rab GTPases (Rab8, Rab10, Rab12, Rab29) to regulate endolysosomal trafficking and ciliogenesis. This broad BP term accurately captures its signaling role.
Reason: LRRK2 functions as a serine/threonine kinase that transduces signals by phosphorylating Rab GTPases, thereby regulating downstream cellular processes. The IBA annotation is appropriate as LRRK2 is clearly involved in intracellular signal transduction via its kinase and GTPase activities.
Supporting Evidence:
PMID:30398148
Parkinson's disease-associated LRRK2 kinase phosphorylates multiple Rab GTPases, including Rab8A and Rab10.
file:human/LRRK2/LRRK2-deep-research-falcon.md
model: Edison Scientific Literature
GO:0005886 plasma membrane
IBA
GO_REF:0000033
KEEP AS NON CORE
Summary: Plasma membrane - LRRK2 can localize to membranes but cytosol is predominant.
Reason: Plasma membrane - LRRK2 can localize to membranes but cytosol is predominant.
GO:0030425 dendrite
IBA
GO_REF:0000033
KEEP AS NON CORE
Summary: Dendrite localization is tissue-specific in neurons, not a core localization.
Reason: Dendrite localization is tissue-specific in neurons, not a core localization.
GO:0007029 endoplasmic reticulum organization
IBA
GO_REF:0000033
ACCEPT
Summary: ER organization is related to LRRK2 role in Sec16A regulation at ER exit sites.
Reason: ER organization is related to LRRK2 role in Sec16A regulation at ER exit sites.
GO:0007030 Golgi organization
IBA
GO_REF:0000033
ACCEPT
Summary: Golgi organization - LRRK2 regulates Golgi-related trafficking through Rab phosphorylation.
Reason: Golgi organization - LRRK2 regulates Golgi-related trafficking through Rab phosphorylation.
GO:0010508 positive regulation of autophagy
IBA
GO_REF:0000033
MARK AS OVER ANNOTATED
Summary: Positive regulation of autophagy is a downstream effect of trafficking disruption, not core function.
Reason: Positive regulation of autophagy is a downstream effect of trafficking disruption, not core function.
GO:0031344 regulation of cell projection organization
IBA
GO_REF:0000033
ACCEPT
Summary: Regulation of cell projection organization - includes ciliogenesis.
Reason: Regulation of cell projection organization - includes ciliogenesis.
GO:0060159 regulation of dopamine receptor signaling pathway
IBA
GO_REF:0000033
KEEP AS NON CORE
Summary: Regulation of dopamine receptor signaling pathway - tissue-specific.
Reason: Regulation of dopamine receptor signaling pathway - tissue-specific.
GO:0060828 regulation of canonical Wnt signaling pathway
IBA
GO_REF:0000033
KEEP AS NON CORE
Summary: Regulation of canonical Wnt signaling pathway - LRRK2 acts as scaffold but not core function.
Reason: Regulation of canonical Wnt signaling pathway - LRRK2 acts as scaffold but not core function.
GO:1900242 regulation of synaptic vesicle endocytosis
IBA
GO_REF:0000033
ACCEPT
Summary: Regulation of synaptic vesicle endocytosis - well-documented LRRK2 function.
Reason: Regulation of synaptic vesicle endocytosis - well-documented LRRK2 function.
GO:0004706 JUN kinase kinase kinase activity
IBA
GO_REF:0000033
UNDECIDED
Summary: JUN kinase kinase kinase activity - reported but may not be physiological core function.
Reason: JUN kinase kinase kinase activity - reported but may not be physiological core function.
GO:0098794 postsynapse
IEA
GO_REF:0000108
KEEP AS NON CORE
Summary: Postsynapse is a tissue-specific neuronal localization.
Reason: Postsynapse is a tissue-specific neuronal localization.
GO:0000139 Golgi membrane
IEA
GO_REF:0000044
ACCEPT
Summary: Golgi membrane localization - LRRK2 is recruited to Golgi membranes where Rab29 resides.
Reason: Golgi membrane localization - LRRK2 is recruited to Golgi membranes where Rab29 resides.
GO:0000166 nucleotide binding
IEA
GO_REF:0000043
ACCEPT
Summary: Nucleotide binding - required for kinase and GTPase activities.
Reason: Nucleotide binding - required for kinase and GTPase activities.
GO:0003924 GTPase activity
IEA
GO_REF:0000116
ACCEPT
Summary: GTPase activity is a core function of LRRK2 ROC domain. The ROC domain binds and hydrolyzes GTP.
Reason: GTPase activity is a core function of LRRK2 ROC domain. The ROC domain binds and hydrolyzes GTP.
GO:0004672 protein kinase activity
IEA
GO_REF:0000120
ACCEPT
Summary: Protein kinase activity is a core function of LRRK2, phosphorylating Rab GTPases and other substrates.
Reason: Protein kinase activity is a core function of LRRK2, phosphorylating Rab GTPases and other substrates.
GO:0004674 protein serine/threonine kinase activity
IEA
GO_REF:0000120
ACCEPT
Summary: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Reason: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
GO:0005096 GTPase activator activity
IEA
GO_REF:0000043
UNDECIDED
Summary: GTPase activator activity - LRRK2 has intrinsic GTPase activity but GAP activity on other GTPases is unclear.
Reason: GTPase activator activity - LRRK2 has intrinsic GTPase activity but GAP activity on other GTPases is unclear.
GO:0005524 ATP binding
IEA
GO_REF:0000120
ACCEPT
Summary: ATP binding is required for LRRK2 kinase activity.
Reason: ATP binding is required for LRRK2 kinase activity.
GO:0005525 GTP binding
IEA
GO_REF:0000120
ACCEPT
Summary: GTP binding is essential for LRRK2 ROC domain function and regulates kinase activity.
Reason: GTP binding is essential for LRRK2 ROC domain function and regulates kinase activity.
GO:0005741 mitochondrial outer membrane
IEA
GO_REF:0000120
KEEP AS NON CORE
Summary: Mitochondrial outer membrane - reported but may be minor.
Reason: Mitochondrial outer membrane - reported but may be minor.
GO:0005764 lysosome
IEA
GO_REF:0000120
ACCEPT
Summary: Lysosome localization is consistent with LRRK2 role in endolysosomal trafficking.
Reason: Lysosome localization is consistent with LRRK2 role in endolysosomal trafficking.
GO:0005768 endosome
IEA
GO_REF:0000120
ACCEPT
Summary: Endosome localization is consistent with LRRK2 role in regulating Rab-dependent trafficking.
Reason: Endosome localization is consistent with LRRK2 role in regulating Rab-dependent trafficking.
GO:0005789 endoplasmic reticulum membrane
IEA
GO_REF:0000044
KEEP AS NON CORE
Summary: ER membrane - related to ER exit site function.
Reason: ER membrane - related to ER exit site function.
GO:0005829 cytosol
IEA
GO_REF:0000117
ACCEPT
Summary: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
Reason: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
GO:0005856 cytoskeleton
IEA
GO_REF:0000044
KEEP AS NON CORE
Summary: Cytoskeleton - microtubule association documented.
Reason: Cytoskeleton - microtubule association documented.
GO:0006914 autophagy
IEA
GO_REF:0000043
MARK AS OVER ANNOTATED
Summary: Autophagy effects are downstream of LRRK2 trafficking disruption, not a direct evolved function.
Reason: Autophagy effects are downstream of LRRK2 trafficking disruption, not a direct evolved function.
GO:0007030 Golgi organization
IEA
GO_REF:0000117
ACCEPT
Summary: Golgi organization - LRRK2 regulates Golgi-related trafficking through Rab phosphorylation.
Reason: Golgi organization - LRRK2 regulates Golgi-related trafficking through Rab phosphorylation.
GO:0007166 cell surface receptor signaling pathway
IEA
GO_REF:0000117
KEEP AS NON CORE
Summary: Cell surface receptor signaling pathway - very broad.
Reason: Cell surface receptor signaling pathway - very broad.
GO:0009653 anatomical structure morphogenesis
IEA
GO_REF:0000117
MARK AS OVER ANNOTATED
Summary: Anatomical structure morphogenesis is very broad.
Reason: Anatomical structure morphogenesis is very broad.
GO:0009968 negative regulation of signal transduction
IEA
GO_REF:0000117
KEEP AS NON CORE
Summary: Negative regulation of signal transduction - broad.
Reason: Negative regulation of signal transduction - broad.
GO:0010468 regulation of gene expression
IEA
GO_REF:0000117
KEEP AS NON CORE
Summary: Regulation of gene expression - very broad downstream effect.
Reason: Regulation of gene expression - very broad downstream effect.
GO:0016192 vesicle-mediated transport
IEA
GO_REF:0000117
ACCEPT
Summary: Vesicle-mediated transport - core function via Rab GTPase phosphorylation regulating trafficking.
Reason: Vesicle-mediated transport - core function via Rab GTPase phosphorylation regulating trafficking.
GO:0016301 kinase activity
IEA
GO_REF:0000043
ACCEPT
Summary: Kinase activity is a core function - LRRK2 is a dual-function kinase/GTPase.
Reason: Kinase activity is a core function - LRRK2 is a dual-function kinase/GTPase.
GO:0016740 transferase activity
IEA
GO_REF:0000043
ACCEPT
Summary: Transferase activity - parent term of kinase activity.
Reason: Transferase activity - parent term of kinase activity.
GO:0016787 hydrolase activity
IEA
GO_REF:0000043
ACCEPT
Summary: Hydrolase activity - parent term of GTPase activity.
Reason: Hydrolase activity - parent term of GTPase activity.
GO:0030154 cell differentiation
IEA
GO_REF:0000043
MARK AS OVER ANNOTATED
Summary: Cell differentiation is very broad.
Reason: Cell differentiation is very broad.
GO:0030159 signaling receptor complex adaptor activity
IEA
GO_REF:0000117
UNDECIDED
Summary: Signaling receptor complex adaptor activity - Wnt scaffold role.
Reason: Signaling receptor complex adaptor activity - Wnt scaffold role.
GO:0030162 regulation of proteolysis
IEA
GO_REF:0000117
KEEP AS NON CORE
Summary: Regulation of proteolysis - broad.
Reason: Regulation of proteolysis - broad.
GO:0030182 neuron differentiation
IEA
GO_REF:0000117
MARK AS OVER ANNOTATED
Summary: Neuron differentiation is developmental.
Reason: Neuron differentiation is developmental.
IEA
GO_REF:0000120
KEEP AS NON CORE
Summary: Axon localization is tissue-specific in neurons.
Reason: Axon localization is tissue-specific in neurons.
GO:0030425 dendrite
IEA
GO_REF:0000120
KEEP AS NON CORE
Summary: Dendrite localization is tissue-specific in neurons, not a core localization.
Reason: Dendrite localization is tissue-specific in neurons, not a core localization.
GO:0030672 synaptic vesicle membrane
IEA
GO_REF:0000044
KEEP AS NON CORE
Summary: Synaptic vesicle membrane is tissue-specific.
Reason: Synaptic vesicle membrane is tissue-specific.
GO:0031410 cytoplasmic vesicle
IEA
GO_REF:0000120
ACCEPT
Summary: Cytoplasmic vesicle - consistent with trafficking role.
Reason: Cytoplasmic vesicle - consistent with trafficking role.
GO:0035640 exploration behavior
IEA
GO_REF:0000117
MARK AS OVER ANNOTATED
Summary: Exploration behavior is a high-level organismal phenotype.
Reason: Exploration behavior is a high-level organismal phenotype.
GO:0042391 regulation of membrane potential
IEA
GO_REF:0000117
KEEP AS NON CORE
Summary: Regulation of membrane potential - neuronal effect.
Reason: Regulation of membrane potential - neuronal effect.
GO:0043195 terminal bouton
IEA
GO_REF:0000117
KEEP AS NON CORE
Summary: Terminal bouton is tissue-specific neuronal localization.
Reason: Terminal bouton is tissue-specific neuronal localization.
GO:0043204 perikaryon
IEA
GO_REF:0000120
KEEP AS NON CORE
Summary: Perikaryon (neuronal cell body) is tissue-specific localization.
Reason: Perikaryon (neuronal cell body) is tissue-specific localization.
GO:0045335 phagocytic vesicle
IEA
GO_REF:0000044
KEEP AS NON CORE
Summary: Phagocytic vesicle - macrophage-specific.
Reason: Phagocytic vesicle - macrophage-specific.
GO:0048513 animal organ development
IEA
GO_REF:0000117
MARK AS OVER ANNOTATED
Summary: Animal organ development is very broad.
Reason: Animal organ development is very broad.
GO:0050804 modulation of chemical synaptic transmission
IEA
GO_REF:0000117
KEEP AS NON CORE
Summary: Modulation of chemical synaptic transmission - tissue-specific.
Reason: Modulation of chemical synaptic transmission - tissue-specific.
GO:0051239 regulation of multicellular organismal process
IEA
GO_REF:0000117
KEEP AS NON CORE
Summary: Regulation of multicellular organismal process - very broad.
Reason: Regulation of multicellular organismal process - very broad.
GO:0051247 positive regulation of protein metabolic process
IEA
GO_REF:0000117
KEEP AS NON CORE
Summary: Positive regulation of protein metabolic process - broad.
Reason: Positive regulation of protein metabolic process - broad.
GO:0060627 regulation of vesicle-mediated transport
IEA
GO_REF:0000117
ACCEPT
Summary: Regulation of vesicle-mediated transport - core trafficking function.
Reason: Regulation of vesicle-mediated transport - core trafficking function.
GO:0070013 intracellular organelle lumen
IEA
GO_REF:0000117
KEEP AS NON CORE
Summary: Intracellular organelle lumen - very broad.
Reason: Intracellular organelle lumen - very broad.
GO:0106310 protein serine kinase activity
IEA
GO_REF:0000116
ACCEPT
Summary: Protein serine kinase activity - LRRK2 phosphorylates serine/threonine residues on Rab substrates.
Reason: Protein serine kinase activity - LRRK2 phosphorylates serine/threonine residues on Rab substrates.
GO:1902531 regulation of intracellular signal transduction
IEA
GO_REF:0000117
KEEP AS NON CORE
Summary: Regulation of intracellular signal transduction - broad.
Reason: Regulation of intracellular signal transduction - broad.
GO:1904887 Wnt signalosome assembly
IEA
GO_REF:0000117
KEEP AS NON CORE
Summary: Wnt signalosome assembly - via LRP6 bridging.
Reason: Wnt signalosome assembly - via LRP6 bridging.
GO:0005515 protein binding
IPI
PMID:16321986
The Parkinson disease causing LRRK2 mutation I2020T is assoc...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:16321986
Dec 1. The Parkinson disease causing LRRK2 mutation I2020T is associated with increased kinase activity.
GO:0005515 protein binding
IPI
PMID:16352719
Leucine-rich repeat kinase 2 (LRRK2) interacts with parkin, ...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:16352719
Leucine-rich repeat kinase 2 (LRRK2) interacts with parkin, and mutant LRRK2 induces neuronal degeneration.
GO:0005515 protein binding
IPI
PMID:17400507
Identification of potential protein interactors of Lrrk2.
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:17400507
Identification of potential protein interactors of Lrrk2.
GO:0005515 protein binding
IPI
PMID:18367605
The chaperone activity of heat shock protein 90 is critical ...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:18367605
The chaperone activity of heat shock protein 90 is critical for maintaining the stability of leucine-rich repeat kinase 2.
GO:0005515 protein binding
IPI
PMID:18445495
LRRK2 regulates synaptic vesicle endocytosis.
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:18445495
Epub 2008 Mar 5. LRRK2 regulates synaptic vesicle endocytosis.
GO:0005515 protein binding
IPI
PMID:19176810
The Parkinson disease protein leucine-rich repeat kinase 2 t...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:19176810
The Parkinson disease protein leucine-rich repeat kinase 2 transduces death signals via Fas-associated protein with death domain and caspase-8 in a cellular model of neurodegeneration.
GO:0005515 protein binding
IPI
PMID:19196961
CHIP regulates leucine-rich repeat kinase-2 ubiquitination, ...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:19196961
CHIP regulates leucine-rich repeat kinase-2 ubiquitination, degradation, and toxicity.
GO:0005515 protein binding
IPI
PMID:19559761
Interaction of elongation factor 1-alpha with leucine-rich r...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:19559761
Interaction of elongation factor 1-alpha with leucine-rich repeat kinase 2 impairs kinase activity and microtubule bundling in vitro.
GO:0005515 protein binding
IPI
PMID:19625296
Mutations in the LRRK2 Roc-COR tandem domain link Parkinson'...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:19625296
Jul 22. Mutations in the LRRK2 Roc-COR tandem domain link Parkinson's disease to Wnt signalling pathways.
GO:0005515 protein binding
IPI
PMID:19712061
Homo- and heterodimerization of ROCO kinases: LRRK2 kinase i...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:19712061
Epub 2009 Aug 27. Homo- and heterodimerization of ROCO kinases: LRRK2 kinase inhibition by the LRRK2 ROCO fragment.
GO:0005515 protein binding
IPI
PMID:20067578
MKK6 binds and regulates expression of Parkinson's disease-r...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:20067578
Epub 2010 Jan 7. MKK6 binds and regulates expression of Parkinson's disease-related protein LRRK2.
GO:0005515 protein binding
IPI
PMID:20144646
Heterodimerization of Lrrk1-Lrrk2: Implications for LRRK2-as...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:20144646
2010 Feb 6. Heterodimerization of Lrrk1-Lrrk2: Implications for LRRK2-associated Parkinson disease.
GO:0005515 protein binding
IPI
PMID:20173330
LRRK2 and the stress response: interaction with MKKs and JNK...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:20173330
LRRK2 and the stress response: interaction with MKKs and JNK-interacting proteins.
GO:0005515 protein binding
IPI
PMID:20642453
14-3-3 binding to LRRK2 is disrupted by multiple Parkinson's...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:20642453
14-3-3 binding to LRRK2 is disrupted by multiple Parkinson's disease-associated mutations and regulates cytoplasmic localization.
GO:0005515 protein binding
IPI
PMID:20671708
Pathogenic LRRK2 negatively regulates microRNA-mediated tran...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:20671708
Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression.
GO:0005515 protein binding
IPI
PMID:21048939
ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange facto...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:21048939
ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange factor for leucine-rich repeat kinase 2.
GO:0005515 protein binding
IPI
PMID:21307259
LRRK2 controls synaptic vesicle storage and mobilization wit...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:21307259
LRRK2 controls synaptic vesicle storage and mobilization within the recycling pool.
GO:0005515 protein binding
IPI
PMID:21370995
Expression of leucine-rich repeat kinase 2 (LRRK2) inhibits ...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:21370995
Expression of leucine-rich repeat kinase 2 (LRRK2) inhibits the processing of uMtCK to induce cell death in a cell culture model system.
GO:0005515 protein binding
IPI
PMID:21454543
Rac1 protein rescues neurite retraction caused by G2019S leu...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:21454543
2011 Mar 16. Rac1 protein rescues neurite retraction caused by G2019S leucine-rich repeat kinase 2 (LRRK2).
GO:0005515 protein binding
IPI
PMID:21658387
LRRK2 directly phosphorylates Akt1 as a possible physiologic...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:21658387
Epub 2011 Jun 2. LRRK2 directly phosphorylates Akt1 as a possible physiological substrate: impairment of the kinase activity by Parkinson's disease-associated mutations.
GO:0005515 protein binding
IPI
PMID:21850687
Mutations in LRRK2 increase phosphorylation of peroxiredoxin...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:21850687
Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death.
GO:0005515 protein binding
IPI
PMID:21983832
The kinase LRRK2 is a regulator of the transcription factor ...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:21983832
The kinase LRRK2 is a regulator of the transcription factor NFAT that modulates the severity of inflammatory bowel disease.
GO:0005515 protein binding
IPI
PMID:22228096
LRRK2 regulates mitochondrial dynamics and function through ...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:22228096
Jan 6. LRRK2 regulates mitochondrial dynamics and function through direct interaction with DLP1.
GO:0005515 protein binding
IPI
PMID:22303461
LRRK2 phosphorylates tubulin-associated tau but not the free...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:22303461
LRRK2 phosphorylates tubulin-associated tau but not the free molecule: LRRK2-mediated regulation of the tau-tubulin association and neurite outgrowth.
GO:0005515 protein binding
IPI
PMID:22363216
GTPase activity and neuronal toxicity of Parkinson's disease...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:22363216
Feb 9. GTPase activity and neuronal toxicity of Parkinson's disease-associated LRRK2 is regulated by ArfGAP1.
GO:0005515 protein binding
IPI
PMID:22612223
The G2385R variant of leucine-rich repeat kinase 2 associate...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:22612223
The G2385R variant of leucine-rich repeat kinase 2 associated with Parkinson's disease is a partial loss-of-function mutation.
GO:0005515 protein binding
IPI
PMID:22639965
Leucine-rich repeat kinase 2 disturbs mitochondrial dynamics...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:22639965
Epub 2012 Jun 22. Leucine-rich repeat kinase 2 disturbs mitochondrial dynamics via Dynamin-like protein.
GO:0005515 protein binding
IPI
PMID:22899650
LRRK2 functions as a Wnt signaling scaffold, bridging cytoso...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:22899650
Aug 16. LRRK2 functions as a Wnt signaling scaffold, bridging cytosolic proteins and membrane-localized LRP6.
GO:0005515 protein binding
IPI
PMID:22952686
Biochemical characterization of highly purified leucine-rich...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:22952686
Biochemical characterization of highly purified leucine-rich repeat kinases 1 and 2 demonstrates formation of homodimers.
GO:0005515 protein binding
IPI
PMID:22998870
LRRK2 controls an EndoA phosphorylation cycle in synaptic en...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:22998870
LRRK2 controls an EndoA phosphorylation cycle in synaptic endocytosis.
GO:0005515 protein binding
IPI
PMID:23075850
Progressive degeneration of human neural stem cells caused b...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:23075850
Progressive degeneration of human neural stem cells caused by pathogenic LRRK2.
GO:0005515 protein binding
IPI
PMID:23183827
LRRK2 interactions with Ξ±-synuclein in Parkinson's disease b...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:23183827
2012 Nov 27. LRRK2 interactions with Ξ±-synuclein in Parkinson's disease brains and in cell models.
GO:0005515 protein binding
IPI
PMID:23241358
GTPase activity regulates kinase activity and cellular pheno...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:23241358
Dec 13. GTPase activity regulates kinase activity and cellular phenotypes of Parkinson's disease-associated LRRK2.
GO:0005515 protein binding
IPI
PMID:23395371
RAB7L1 interacts with LRRK2 to modify intraneuronal protein ...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:23395371
RAB7L1 interacts with LRRK2 to modify intraneuronal protein sorting and Parkinson's disease risk.
GO:0005515 protein binding
IPI
PMID:23455607
Interplay of LRRK2 with chaperone-mediated autophagy.
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:23455607
Interplay of LRRK2 with chaperone-mediated autophagy.
GO:0005515 protein binding
IPI
PMID:23813973
Inhibition of excessive mitochondrial fission reduced aberra...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:23813973
Jun 27. Inhibition of excessive mitochondrial fission reduced aberrant autophagy and neuronal damage caused by LRRK2 G2019S mutation.
GO:0005515 protein binding
IPI
PMID:23937259
Identification of protein phosphatase 1 as a regulator of th...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:23937259
Identification of protein phosphatase 1 as a regulator of the LRRK2 phosphorylation cycle.
GO:0005515 protein binding
IPI
PMID:23949442
LRRK2 phosphorylates Snapin and inhibits interaction of Snap...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:23949442
LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25.
GO:0005515 protein binding
IPI
PMID:24113872
LRRK2 phosphorylates novel tau epitopes and promotes tauopat...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:24113872
2013 Oct 11. LRRK2 phosphorylates novel tau epitopes and promotes tauopathy.
GO:0005515 protein binding
IPI
PMID:24165324
Leucine-rich repeat kinaseΒ 2 regulates tau phosphorylation t...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:24165324
Leucine-rich repeat kinase 2 regulates tau phosphorylation through direct activation of glycogen synthase kinase-3Ξ².
GO:0005515 protein binding
IPI
PMID:24275654
A direct interaction between leucine-rich repeat kinase 2 an...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:24275654
2013 Nov 25. A direct interaction between leucine-rich repeat kinase 2 and specific Ξ²-tubulin isoforms regulates tubulin acetylation.
GO:0005515 protein binding
IPI
PMID:24282027
Functional interaction of Parkinson's disease-associated LRR...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:24282027
Nov 26. Functional interaction of Parkinson's disease-associated LRRK2 with members of the dynamin GTPase superfamily.
GO:0005515 protein binding
IPI
PMID:24351927
Parkinson-related LRRK2 mutation R1441C/G/H impairs PKA phos...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:24351927
Parkinson-related LRRK2 mutation R1441C/G/H impairs PKA phosphorylation of LRRK2 and disrupts its interaction with 14-3-3.
GO:0005515 protein binding
IPI
PMID:24459295
Thiol peroxidases ameliorate LRRK2 mutant-induced mitochondr...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:24459295
Jan 23. Thiol peroxidases ameliorate LRRK2 mutant-induced mitochondrial and dopaminergic neuronal degeneration in Drosophila.
GO:0005515 protein binding
IPI
PMID:24464040
LRRK2 regulates synaptogenesis and dopamine receptor activat...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:24464040
LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity.
GO:0005515 protein binding
IPI
PMID:24510904
Unbiased screen for interactors of leucine-rich repeat kinas...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:24510904
Unbiased screen for interactors of leucine-rich repeat kinase 2 supports a common pathway for sporadic and familial Parkinson disease.
GO:0005515 protein binding
IPI
PMID:24687852
Leucine-rich repeat kinase 2 binds to neuronal vesicles thro...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:24687852
Mar 31. Leucine-rich repeat kinase 2 binds to neuronal vesicles through protein interactions mediated by its C-terminal WD40 domain.
GO:0005515 protein binding
IPI
PMID:24695735
The Parkinson disease-linked LRRK2 protein mutation I2020T s...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:24695735
2014 Apr 2. The Parkinson disease-linked LRRK2 protein mutation I2020T stabilizes an active state conformation leading to increased kinase activity.
GO:0005515 protein binding
IPI
PMID:24725412
Ribosomal protein s15 phosphorylation mediates LRRK2 neurode...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:24725412
Ribosomal protein s15 phosphorylation mediates LRRK2 neurodegeneration in Parkinson's disease.
GO:0005515 protein binding
IPI
PMID:24754922
MAP1B rescues LRRK2 mutant-mediated cytotoxicity.
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:24754922
MAP1B rescues LRRK2 mutant-mediated cytotoxicity.
GO:0005515 protein binding
IPI
PMID:24794857
A Parkinson's disease gene regulatory network identifies the...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:24794857
May 2. A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity.
GO:0005515 protein binding
IPI
PMID:24904275
LRRK2 kinase activity regulates synaptic vesicle trafficking...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:24904275
eCollection 2014. LRRK2 kinase activity regulates synaptic vesicle trafficking and neurotransmitter release through modulation of LRRK2 macro-molecular complex.
GO:0005515 protein binding
IPI
PMID:24947832
Differential protein-protein interactions of LRRK1 and LRRK2...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:24947832
Differential protein-protein interactions of LRRK1 and LRRK2 indicate roles in distinct cellular signaling pathways.
GO:0005515 protein binding
IPI
PMID:25009464
LRRK2 kinase activity and biology are not uniformly predicte...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:25009464
eCollection 2014. LRRK2 kinase activity and biology are not uniformly predicted by its autophosphorylation and cellular phosphorylation site status.
GO:0005515 protein binding
IPI
PMID:25201882
Leucine-rich repeat kinase 2 regulates Sec16A at ER exit sit...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:25201882
Sep 8. Leucine-rich repeat kinase 2 regulates Sec16A at ER exit sites to allow ER-Golgi export.
GO:0005515 protein binding
IPI
PMID:25360523
LRRK2 transport is regulated by its novel interacting partne...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:25360523
eCollection 2014. LRRK2 transport is regulated by its novel interacting partner Rab32.
GO:0005515 protein binding
IPI
PMID:25446991
Threonine 56 phosphorylation of Bcl-2 is required for LRRK2 ...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:25446991
Threonine 56 phosphorylation of Bcl-2 is required for LRRK2 G2019S-induced mitochondrial depolarization and autophagy.
GO:0005515 protein binding
IPI
PMID:25500533
Phosphorylation of LRRK2 by casein kinase 1Ξ± regulates trans...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:25500533
Phosphorylation of LRRK2 by casein kinase 1Ξ± regulates trans-Golgi clustering via differential interaction with ARHGEF7.
GO:0005515 protein binding
IPI
PMID:25501810
LRRK2 functions in synaptic vesicle endocytosis through a ki...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:25501810
LRRK2 functions in synaptic vesicle endocytosis through a kinase-dependent mechanism.
GO:0005515 protein binding
IPI
PMID:25605758
An early endosome regulator, Rab5b, is an LRRK2 kinase subst...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:25605758
An early endosome regulator, Rab5b, is an LRRK2 kinase substrate.
GO:0005515 protein binding
IPI
PMID:26824392
Phosphoproteomics reveals that Parkinson's disease kinase LR...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:26824392
Phosphoproteomics reveals that Parkinson's disease kinase LRRK2 regulates a subset of Rab GTPases.
GO:0005515 protein binding
IPI
PMID:26894577
Identification of protein phosphatase 2A as an interacting p...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:26894577
Identification of protein phosphatase 2A as an interacting protein of leucine-rich repeat kinase 2.
GO:0005515 protein binding
IPI
PMID:27013965
Protective LRRK2 R1398H Variant Enhances GTPase and Wnt Sign...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:27013965
eCollection 2016. Protective LRRK2 R1398H Variant Enhances GTPase and Wnt Signaling Activity.
GO:0005515 protein binding
IPI
PMID:27273569
Ubiqutination via K27 and K29 chains signals aggregation and...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:27273569
Ubiqutination via K27 and K29 chains signals aggregation and neuronal protection of LRRK2 by WSB1.
GO:0005515 protein binding
IPI
PMID:27314038
G2385R and I2020T Mutations Increase LRRK2 GTPase Activity.
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:27314038
G2385R and I2020T Mutations Increase LRRK2 GTPase Activity.
GO:0005515 protein binding
IPI
PMID:27357661
Structural model of the dimeric Parkinson's protein LRRK2 re...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:27357661
Structural model of the dimeric Parkinson's protein LRRK2 reveals a compact architecture involving distant interdomain contacts.
GO:0005515 protein binding
IPI
PMID:27424887
LRRK2 and RAB7L1 coordinately regulate axonal morphology and...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:27424887
LRRK2 and RAB7L1 coordinately regulate axonal morphology and lysosome integrity in diverse cellular contexts.
GO:0005515 protein binding
IPI
PMID:28202711
Structural interface between LRRK2 and 14-3-3 protein.
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:28202711
Structural interface between LRRK2 and 14-3-3 protein.
GO:0005515 protein binding
IPI
PMID:29513927
Comparative Protein Interaction Network Analysis Identifies ...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:29513927
Apr 17. Comparative Protein Interaction Network Analysis Identifies Shared and Distinct Functions for the Human ROCO Proteins.
GO:0005515 protein binding
IPI
PMID:29519959
P62/SQSTM1 is a novel leucine-rich repeat kinase 2 (LRRK2) s...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:29519959
P62/SQSTM1 is a novel leucine-rich repeat kinase 2 (LRRK2) substrate that enhances neuronal toxicity.
GO:0005515 protein binding
IPI
PMID:29541021
The LRRK2 Variant E193K Prevents Mitochondrial Fission Upon ...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:29541021
eCollection 2018. The LRRK2 Variant E193K Prevents Mitochondrial Fission Upon MPP+ Treatment by Altering LRRK2 Binding to DRP1.
GO:0005515 protein binding
IPI
PMID:31046837
Parkinson's disease-associated LRRK2-G2019S mutant acts thro...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:31046837
Parkinson's disease-associated LRRK2-G2019S mutant acts through regulation of SERCA activity to control ER stress in astrocytes.
GO:0005515 protein binding
IPI
PMID:31552791
LRRK2 binds to the Rab32 subfamily in a GTP-dependent manner...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:31552791
Epub 2019 Sep 25. LRRK2 binds to the Rab32 subfamily in a GTP-dependent manner via its armadillo domain.
GO:0005515 protein binding
IPI
PMID:32017888
Structural Basis for Rab8a Recruitment of RILPL2 via LRRK2 P...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:32017888
2020 Feb 3. Structural Basis for Rab8a Recruitment of RILPL2 via LRRK2 Phosphorylation of Switch 2.
GO:0005515 protein binding
IPI
PMID:32814053
Interactome Mapping Provides a Network of Neurodegenerative ...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:32814053
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
GO:0005515 protein binding
IPI
PMID:33938021
Genomewide Association Studies of LRRK2 Modifiers of Parkins...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:33938021
Genomewide Association Studies of LRRK2 Modifiers of Parkinson's Disease.
GO:0042802 identical protein binding
IPI
PMID:16321986
The Parkinson disease causing LRRK2 mutation I2020T is assoc...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:16321986
Dec 1. The Parkinson disease causing LRRK2 mutation I2020T is associated with increased kinase activity.
GO:0042802 identical protein binding
IPI
PMID:18230735
Structure of the ROC domain from the Parkinson's disease-ass...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:18230735
Structure of the ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2 reveals a dimeric GTPase.
GO:0042802 identical protein binding
IPI
PMID:18397888
The Parkinson disease-associated leucine-rich repeat kinase ...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:18397888
2008 Apr 8. The Parkinson disease-associated leucine-rich repeat kinase 2 (LRRK2) is a dimer that undergoes intramolecular autophosphorylation.
GO:0042802 identical protein binding
IPI
PMID:19712061
Homo- and heterodimerization of ROCO kinases: LRRK2 kinase i...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:19712061
Epub 2009 Aug 27. Homo- and heterodimerization of ROCO kinases: LRRK2 kinase inhibition by the LRRK2 ROCO fragment.
GO:0042802 identical protein binding
IPI
PMID:19733152
The Parkinson's disease kinase LRRK2 autophosphorylates its ...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:19733152
The Parkinson's disease kinase LRRK2 autophosphorylates its GTPase domain at multiple sites.
GO:0042802 identical protein binding
IPI
PMID:20515039
Membrane localization of LRRK2 is associated with increased ...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:20515039
Membrane localization of LRRK2 is associated with increased formation of the highly active LRRK2 dimer and changes in its phosphorylation.
GO:0042802 identical protein binding
IPI
PMID:21073465
Insight into the mode of action of the LRRK2 Y1699C pathogen...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:21073465
Insight into the mode of action of the LRRK2 Y1699C pathogenic mutant.
GO:0042802 identical protein binding
IPI
PMID:22363216
GTPase activity and neuronal toxicity of Parkinson's disease...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:22363216
Feb 9. GTPase activity and neuronal toxicity of Parkinson's disease-associated LRRK2 is regulated by ArfGAP1.
GO:0042802 identical protein binding
IPI
PMID:22952686
Biochemical characterization of highly purified leucine-rich...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:22952686
Biochemical characterization of highly purified leucine-rich repeat kinases 1 and 2 demonstrates formation of homodimers.
GO:0042802 identical protein binding
IPI
PMID:23220480
Dominant-negative effects of LRRK2 heterodimers: a possible ...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:23220480
Dominant-negative effects of LRRK2 heterodimers: a possible mechanism of neurodegeneration in Parkinson's disease caused by LRRK2 I2020T mutation.
GO:0042802 identical protein binding
IPI
PMID:23241358
GTPase activity regulates kinase activity and cellular pheno...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:23241358
Dec 13. GTPase activity regulates kinase activity and cellular phenotypes of Parkinson's disease-associated LRRK2.
GO:0042802 identical protein binding
IPI
PMID:24275654
A direct interaction between leucine-rich repeat kinase 2 an...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:24275654
2013 Nov 25. A direct interaction between leucine-rich repeat kinase 2 and specific Ξ²-tubulin isoforms regulates tubulin acetylation.
GO:0042802 identical protein binding
IPI
PMID:24591621
Parkinson disease-associated mutation R1441H in LRRK2 prolon...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:24591621
Parkinson disease-associated mutation R1441H in LRRK2 prolongs the "active state" of its GTPase domain.
GO:0042802 identical protein binding
IPI
PMID:24904275
LRRK2 kinase activity regulates synaptic vesicle trafficking...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:24904275
eCollection 2014. LRRK2 kinase activity regulates synaptic vesicle trafficking and neurotransmitter release through modulation of LRRK2 macro-molecular complex.
GO:0042802 identical protein binding
IPI
PMID:26824392
Phosphoproteomics reveals that Parkinson's disease kinase LR...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:26824392
Phosphoproteomics reveals that Parkinson's disease kinase LRRK2 regulates a subset of Rab GTPases.
GO:0042802 identical protein binding
IPI
PMID:27013965
Protective LRRK2 R1398H Variant Enhances GTPase and Wnt Sign...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:27013965
eCollection 2016. Protective LRRK2 R1398H Variant Enhances GTPase and Wnt Signaling Activity.
GO:0042802 identical protein binding
IPI
PMID:27357661
Structural model of the dimeric Parkinson's protein LRRK2 re...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:27357661
Structural model of the dimeric Parkinson's protein LRRK2 reveals a compact architecture involving distant interdomain contacts.
GO:0005737 cytoplasm
IEA
GO_REF:0000107
ACCEPT
Summary: Cytoplasm is a major localization for LRRK2.
Reason: Cytoplasm is a major localization for LRRK2.
GO:0005743 mitochondrial inner membrane
IEA
GO_REF:0000107
KEEP AS NON CORE
Summary: Mitochondrial inner membrane - reported but may be minor.
Reason: Mitochondrial inner membrane - reported but may be minor.
GO:0005759 mitochondrial matrix
IEA
GO_REF:0000107
KEEP AS NON CORE
Summary: Mitochondrial matrix - reported but may be minor.
Reason: Mitochondrial matrix - reported but may be minor.
GO:0005783 endoplasmic reticulum
IEA
GO_REF:0000107
ACCEPT
Summary: Endoplasmic reticulum - documented localization at ER exit sites.
Reason: Endoplasmic reticulum - documented localization at ER exit sites.
GO:0005794 Golgi apparatus
IEA
GO_REF:0000120
ACCEPT
Summary: Golgi apparatus localization is supported - LRRK2 is recruited to Golgi by Rab29.
Reason: Golgi apparatus localization is supported - LRRK2 is recruited to Golgi by Rab29.
GO:0005802 trans-Golgi network
IEA
GO_REF:0000107
ACCEPT
Summary: Trans-Golgi network localization is supported by Rab29-mediated recruitment studies.
Reason: Trans-Golgi network localization is supported by Rab29-mediated recruitment studies.
GO:0005886 plasma membrane
IEA
GO_REF:0000120
KEEP AS NON CORE
Summary: Plasma membrane - LRRK2 can localize to membranes but cytosol is predominant.
Reason: Plasma membrane - LRRK2 can localize to membranes but cytosol is predominant.
GO:0007029 endoplasmic reticulum organization
IEA
GO_REF:0000120
ACCEPT
Summary: ER organization is related to LRRK2 role in Sec16A regulation at ER exit sites.
Reason: ER organization is related to LRRK2 role in Sec16A regulation at ER exit sites.
GO:0007283 spermatogenesis
IEA
GO_REF:0000107
MARK AS OVER ANNOTATED
Summary: Spermatogenesis is tissue-specific.
Reason: Spermatogenesis is tissue-specific.
GO:0008021 synaptic vesicle
IEA
GO_REF:0000107
KEEP AS NON CORE
Summary: Synaptic vesicle is tissue-specific in neurons.
Reason: Synaptic vesicle is tissue-specific in neurons.
GO:0008104 intracellular protein localization
IEA
GO_REF:0000107
ACCEPT
Summary: Intracellular protein localization - broad but relevant to trafficking role.
Reason: Intracellular protein localization - broad but relevant to trafficking role.
GO:0010508 positive regulation of autophagy
IEA
GO_REF:0000120
MARK AS OVER ANNOTATED
Summary: Positive regulation of autophagy is a downstream effect of trafficking disruption, not core function.
Reason: Positive regulation of autophagy is a downstream effect of trafficking disruption, not core function.
GO:0010977 negative regulation of neuron projection development
IEA
GO_REF:0000107
KEEP AS NON CORE
Summary: Negative regulation of neuron projection development - neuronal.
Reason: Negative regulation of neuron projection development - neuronal.
GO:0021756 striatum development
IEA
GO_REF:0000107
MARK AS OVER ANNOTATED
Summary: Striatum development is tissue-specific developmental effect.
Reason: Striatum development is tissue-specific developmental effect.
GO:0031267 small GTPase binding
IEA
GO_REF:0000120
ACCEPT
Summary: Small GTPase binding - LRRK2 binds Rab substrates and other small GTPases.
Reason: Small GTPase binding - LRRK2 binds Rab substrates and other small GTPases.
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
IEA
GO_REF:0000107
KEEP AS NON CORE
Summary: Positive regulation of proteasomal ubiquitin-dependent protein catabolic process - downstream.
Reason: Positive regulation of proteasomal ubiquitin-dependent protein catabolic process - downstream.
GO:0032760 positive regulation of tumor necrosis factor production
IEA
GO_REF:0000107
MARK AS OVER ANNOTATED
Summary: Positive regulation of TNF production is downstream immune effect.
Reason: Positive regulation of TNF production is downstream immune effect.
GO:0035556 intracellular signal transduction
IEA
GO_REF:0000120
ACCEPT
Summary: Intracellular signal transduction - LRRK2 functions as a kinase that phosphorylates Rab GTPases.
Reason: Intracellular signal transduction - LRRK2 functions as a kinase that phosphorylates Rab GTPases.
GO:0035564 regulation of kidney size
IEA
GO_REF:0000107
MARK AS OVER ANNOTATED
Summary: Regulation of kidney size is tissue-specific.
Reason: Regulation of kidney size is tissue-specific.
GO:0035641 locomotory exploration behavior
IEA
GO_REF:0000107
MARK AS OVER ANNOTATED
Summary: Locomotory exploration behavior is organismal phenotype.
Reason: Locomotory exploration behavior is organismal phenotype.
GO:0042802 identical protein binding
IEA
GO_REF:0000120
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
GO:0043025 neuronal cell body
IEA
GO_REF:0000107
KEEP AS NON CORE
Summary: Neuronal cell body is tissue-specific localization.
Reason: Neuronal cell body is tissue-specific localization.
GO:0045121 membrane raft
IEA
GO_REF:0000120
KEEP AS NON CORE
Summary: Membrane raft - reported localization.
Reason: Membrane raft - reported localization.
GO:0045202 synapse
IEA
GO_REF:0000107
KEEP AS NON CORE
Summary: Synapse is tissue-specific neuronal localization.
Reason: Synapse is tissue-specific neuronal localization.
GO:0051018 protein kinase A binding
IEA
GO_REF:0000120
KEEP AS NON CORE
Summary: Protein kinase A binding - interaction documented but not core.
Reason: Protein kinase A binding - interaction documented but not core.
GO:0051966 regulation of synaptic transmission, glutamatergic
IEA
GO_REF:0000107
KEEP AS NON CORE
Summary: Regulation of synaptic transmission, glutamatergic - tissue-specific.
Reason: Regulation of synaptic transmission, glutamatergic - tissue-specific.
GO:0060079 excitatory postsynaptic potential
IEA
GO_REF:0000107
KEEP AS NON CORE
Summary: Excitatory postsynaptic potential - tissue-specific.
Reason: Excitatory postsynaptic potential - tissue-specific.
GO:0060159 regulation of dopamine receptor signaling pathway
IEA
GO_REF:0000120
KEEP AS NON CORE
Summary: Regulation of dopamine receptor signaling pathway - tissue-specific.
Reason: Regulation of dopamine receptor signaling pathway - tissue-specific.
GO:0060628 regulation of ER to Golgi vesicle-mediated transport
IEA
GO_REF:0000107
ACCEPT
Summary: Regulation of ER to Golgi vesicle-mediated transport - via Sec16A regulation.
Reason: Regulation of ER to Golgi vesicle-mediated transport - via Sec16A regulation.
GO:0060828 regulation of canonical Wnt signaling pathway
IEA
GO_REF:0000120
KEEP AS NON CORE
Summary: Regulation of canonical Wnt signaling pathway - LRRK2 acts as scaffold but not core function.
Reason: Regulation of canonical Wnt signaling pathway - LRRK2 acts as scaffold but not core function.
GO:0061001 regulation of dendritic spine morphogenesis
IEA
GO_REF:0000120
KEEP AS NON CORE
Summary: Regulation of dendritic spine morphogenesis - neuronal effect.
Reason: Regulation of dendritic spine morphogenesis - neuronal effect.
GO:0070971 endoplasmic reticulum exit site
IEA
GO_REF:0000120
ACCEPT
Summary: ER exit site localization - LRRK2 regulates Sec16A at ER exit sites.
Reason: ER exit site localization - LRRK2 regulates Sec16A at ER exit sites.
GO:0070973 protein localization to endoplasmic reticulum exit site
IEA
GO_REF:0000120
MODIFY
Summary: Protein localization to ER exit site - LRRK2 regulates Sec16A; the annotated term is now obsolete.
Reason: GO:0070973 protein localization to endoplasmic reticulum exit site was obsoleted (GO release 2026-07-26) because it conflated distinct processes; GO suggests context-specific replacements. PMID:25201882 shows LRRK2 anchors Sec16A at ER exit sites and that this regulates anterograde ER-to-Golgi export (LRRK2 depletion disperses Sec16A and impairs ER export), so the finding is best captured by GO:0060628 regulation of ER to Golgi vesicle-mediated transport.
Supporting Evidence:
PMID:25201882
LRRK2 regulates the anterograde ER-Golgi transport through anchoring Sec16A at the endoplasmic reticulum exit sites
GO:0090394 negative regulation of excitatory postsynaptic potential
IEA
GO_REF:0000107
KEEP AS NON CORE
Summary: Negative regulation of excitatory postsynaptic potential - tissue-specific.
Reason: Negative regulation of excitatory postsynaptic potential - tissue-specific.
GO:0098978 glutamatergic synapse
IEA
GO_REF:0000107
KEEP AS NON CORE
Summary: Glutamatergic synapse is tissue-specific.
Reason: Glutamatergic synapse is tissue-specific.
GO:0099523 presynaptic cytosol
IEA
GO_REF:0000120
KEEP AS NON CORE
Summary: Presynaptic cytosol is tissue-specific.
Reason: Presynaptic cytosol is tissue-specific.
GO:0140058 neuron projection arborization
IEA
GO_REF:0000107
MARK AS OVER ANNOTATED
Summary: Neuron projection arborization is developmental.
Reason: Neuron projection arborization is developmental.
GO:0141161 regulation of cAMP/PKA signal transduction
IEA
GO_REF:0000107
KEEP AS NON CORE
Summary: Regulation of cAMP/PKA signal transduction - related to dopamine signaling.
Reason: Regulation of cAMP/PKA signal transduction - related to dopamine signaling.
GO:1900242 regulation of synaptic vesicle endocytosis
IEA
GO_REF:0000107
ACCEPT
Summary: Regulation of synaptic vesicle endocytosis - well-documented LRRK2 function.
Reason: Regulation of synaptic vesicle endocytosis - well-documented LRRK2 function.
GO:1900244 positive regulation of synaptic vesicle endocytosis
IEA
GO_REF:0000107
KEEP AS NON CORE
Summary: Positive regulation of synaptic vesicle endocytosis - tissue-specific.
Reason: Positive regulation of synaptic vesicle endocytosis - tissue-specific.
GO:1902803 regulation of synaptic vesicle transport
IEA
GO_REF:0000107
KEEP AS NON CORE
Summary: Regulation of synaptic vesicle transport - tissue-specific.
Reason: Regulation of synaptic vesicle transport - tissue-specific.
GO:1903980 positive regulation of microglial cell activation
IEA
GO_REF:0000107
MARK AS OVER ANNOTATED
Summary: Positive regulation of microglial cell activation is downstream immune effect.
Reason: Positive regulation of microglial cell activation is downstream immune effect.
GO:1904644 cellular response to curcumin
IEA
GO_REF:0000107
MARK AS OVER ANNOTATED
Summary: Cellular response to curcumin is an experimental condition response.
Reason: Cellular response to curcumin is an experimental condition response.
GO:1904713 beta-catenin destruction complex binding
IEA
GO_REF:0000107
KEEP AS NON CORE
Summary: Beta-catenin destruction complex binding - scaffold role.
Reason: Beta-catenin destruction complex binding - scaffold role.
GO:1990904 ribonucleoprotein complex
IEA
GO_REF:0000107
KEEP AS NON CORE
Summary: Ribonucleoprotein complex - specific interaction.
Reason: Ribonucleoprotein complex - specific interaction.
GO:1990909 Wnt signalosome
IEA
GO_REF:0000120
KEEP AS NON CORE
Summary: Wnt signalosome localization - scaffold role.
Reason: Wnt signalosome localization - scaffold role.
GO:0004674 protein serine/threonine kinase activity
EXP
PMID:26751287
The LINK-A lncRNA activates normoxic HIF1Ξ± signalling in tri...
ACCEPT
Summary: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Reason: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Supporting Evidence:
PMID:26751287
The LINK-A lncRNA activates normoxic HIF1Ξ± signalling in triple-negative breast cancer.
GO:1905504 negative regulation of motile cilium assembly
TAS
PMID:30398148
A pathway for Parkinson's Disease LRRK2 kinase to block prim...
ACCEPT
Summary: Negative regulation of motile cilium assembly - core function via Rab8/Rab10/RILPL1 axis.
Reason: Negative regulation of motile cilium assembly - core function via Rab8/Rab10/RILPL1 axis.
Supporting Evidence:
PMID:30398148
A pathway for Parkinson's Disease LRRK2 kinase to block primary cilia and Sonic hedgehog signaling in the brain.
GO:0035542 regulation of SNARE complex assembly
IMP
PMID:23949442
LRRK2 phosphorylates Snapin and inhibits interaction of Snap...
KEEP AS NON CORE
Summary: Regulation of SNARE complex assembly - related to vesicle function.
Reason: Regulation of SNARE complex assembly - related to vesicle function.
Supporting Evidence:
PMID:23949442
LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25.
GO:0043410 positive regulation of MAPK cascade
IMP
PMID:21857923
Dysregulated LRRK2 signaling in response to endoplasmic reti...
UNDECIDED
Summary: Positive regulation of MAPK cascade - may be indirect.
Reason: Positive regulation of MAPK cascade - may be indirect.
Supporting Evidence:
PMID:21857923
Dysregulated LRRK2 signaling in response to endoplasmic reticulum stress leads to dopaminergic neuron degeneration in C.
GO:0043410 positive regulation of MAPK cascade
IMP
PMID:23628791
Down-regulation of LRRK2 in control and DAT transfected HEK ...
UNDECIDED
Summary: Positive regulation of MAPK cascade - may be indirect.
Reason: Positive regulation of MAPK cascade - may be indirect.
Supporting Evidence:
PMID:23628791
2013 Apr 27. Down-regulation of LRRK2 in control and DAT transfected HEK cells increases manganese-induced oxidative stress and cell toxicity.
GO:1901030 positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
IDA
PMID:21370995
Expression of leucine-rich repeat kinase 2 (LRRK2) inhibits ...
MARK AS OVER ANNOTATED
Summary: Positive regulation of mitochondrial outer membrane permeabilization in apoptotic signaling is disease-related.
Reason: Positive regulation of mitochondrial outer membrane permeabilization in apoptotic signaling is disease-related.
Supporting Evidence:
PMID:21370995
Expression of leucine-rich repeat kinase 2 (LRRK2) inhibits the processing of uMtCK to induce cell death in a cell culture model system.
GO:0042802 identical protein binding
IPI
PMID:38127736
Rab29-dependent asymmetrical activation of leucine-rich repe...
ACCEPT
Summary: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Reason: Identical protein binding - LRRK2 forms homodimers, important for regulation.
Supporting Evidence:
PMID:38127736
2023 Dec 21. Rab29-dependent asymmetrical activation of leucine-rich repeat kinase 2.
GO:0005515 protein binding
IPI
PMID:38858457
Systematic rare variant analyses identify RAB32 as a suscept...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:38858457
2024 Jun 10. Systematic rare variant analyses identify RAB32 as a susceptibility gene for familial Parkinson's disease.
GO:0141161 regulation of cAMP/PKA signal transduction
ISS
GO_REF:0000024
KEEP AS NON CORE
Summary: Regulation of cAMP/PKA signal transduction - related to dopamine signaling.
Reason: Regulation of cAMP/PKA signal transduction - related to dopamine signaling.
GO:0007266 Rho protein signal transduction
IDA
PMID:25500533
Phosphorylation of LRRK2 by casein kinase 1Ξ± regulates trans...
UNDECIDED
Summary: Rho protein signal transduction - may be downstream effect.
Reason: Rho protein signal transduction - may be downstream effect.
Supporting Evidence:
PMID:25500533
Phosphorylation of LRRK2 by casein kinase 1Ξ± regulates trans-Golgi clustering via differential interaction with ARHGEF7.
GO:0034614 cellular response to reactive oxygen species
IMP
PMID:24576675
LRRK2, but not pathogenic mutants, protects against H2O2 str...
MARK AS OVER ANNOTATED
Summary: Cellular response to reactive oxygen species is disease-related.
Reason: Cellular response to reactive oxygen species is disease-related.
Supporting Evidence:
PMID:24576675
LRRK2, but not pathogenic mutants, protects against H2O2 stress depending on mitochondrial function and endocytosis in a yeast model.
GO:2000377 regulation of reactive oxygen species metabolic process
IMP
PMID:24576675
LRRK2, but not pathogenic mutants, protects against H2O2 str...
MARK AS OVER ANNOTATED
Summary: Regulation of ROS metabolic process is downstream.
Reason: Regulation of ROS metabolic process is downstream.
Supporting Evidence:
PMID:24576675
LRRK2, but not pathogenic mutants, protects against H2O2 stress depending on mitochondrial function and endocytosis in a yeast model.
GO:0004674 protein serine/threonine kinase activity
IDA
PMID:27830463
LRRK2 enhances Nod1/2-mediated inflammatory cytokine product...
ACCEPT
Summary: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Reason: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Supporting Evidence:
PMID:27830463
Nov 9. LRRK2 enhances Nod1/2-mediated inflammatory cytokine production by promoting Rip2 phosphorylation.
GO:0030159 signaling receptor complex adaptor activity
IDA
PMID:22899650
LRRK2 functions as a Wnt signaling scaffold, bridging cytoso...
UNDECIDED
Summary: Signaling receptor complex adaptor activity - Wnt scaffold role.
Reason: Signaling receptor complex adaptor activity - Wnt scaffold role.
Supporting Evidence:
PMID:22899650
Aug 16. LRRK2 functions as a Wnt signaling scaffold, bridging cytosolic proteins and membrane-localized LRP6.
GO:0039706 co-receptor binding
TAS
PMID:24115276
The regulation and deregulation of Wnt signaling by PARK gen...
UNDECIDED
Summary: Co-receptor binding - Wnt-related, needs verification.
Reason: Co-receptor binding - Wnt-related, needs verification.
Supporting Evidence:
PMID:24115276
The regulation and deregulation of Wnt signaling by PARK genes in health and disease.
GO:0090263 positive regulation of canonical Wnt signaling pathway
IGI
PMID:22899650
LRRK2 functions as a Wnt signaling scaffold, bridging cytoso...
KEEP AS NON CORE
Summary: Positive regulation of canonical Wnt signaling pathway - scaffold role.
Reason: Positive regulation of canonical Wnt signaling pathway - scaffold role.
Supporting Evidence:
PMID:22899650
Aug 16. LRRK2 functions as a Wnt signaling scaffold, bridging cytosolic proteins and membrane-localized LRP6.
GO:0097413 Lewy body
IDA
PMID:27273569
Ubiqutination via K27 and K29 chains signals aggregation and...
KEEP AS NON CORE
Summary: Lewy body - disease-specific pathological structure.
Reason: Lewy body - disease-specific pathological structure.
Supporting Evidence:
PMID:27273569
Ubiqutination via K27 and K29 chains signals aggregation and neuronal protection of LRRK2 by WSB1.
GO:0004706 JUN kinase kinase kinase activity
IDA
PMID:19302196
The Parkinson disease-associated protein kinase LRRK2 exhibi...
UNDECIDED
Summary: JUN kinase kinase kinase activity - reported but may not be physiological core function.
Reason: JUN kinase kinase kinase activity - reported but may not be physiological core function.
Supporting Evidence:
PMID:19302196
Epub 2009 Mar 3. The Parkinson disease-associated protein kinase LRRK2 exhibits MAPKKK activity and phosphorylates MKK3/6 and MKK4/7, in vitro.
GO:0004709 MAP kinase kinase kinase activity
IDA
PMID:19302196
The Parkinson disease-associated protein kinase LRRK2 exhibi...
UNDECIDED
Summary: MAP kinase kinase kinase activity - reported in vitro but physiological relevance unclear.
Reason: MAP kinase kinase kinase activity - reported in vitro but physiological relevance unclear.
Supporting Evidence:
PMID:19302196
Epub 2009 Mar 3. The Parkinson disease-associated protein kinase LRRK2 exhibits MAPKKK activity and phosphorylates MKK3/6 and MKK4/7, in vitro.
GO:0007254 JNK cascade
IDA
PMID:19302196
The Parkinson disease-associated protein kinase LRRK2 exhibi...
UNDECIDED
Summary: JNK cascade - reported but physiological relevance as direct function unclear.
Reason: JNK cascade - reported but physiological relevance as direct function unclear.
Supporting Evidence:
PMID:19302196
Epub 2009 Mar 3. The Parkinson disease-associated protein kinase LRRK2 exhibits MAPKKK activity and phosphorylates MKK3/6 and MKK4/7, in vitro.
GO:0000287 magnesium ion binding
IMP
PMID:28720718
Phosphorylation of amyloid precursor protein by mutant LRRK2...
UNDECIDED
Summary: GO term GO:0000287 requires further review - no pre-defined decision available.
Reason: Term not in curated decision list, requires manual review.
Supporting Evidence:
PMID:28720718
Phosphorylation of amyloid precursor protein by mutant LRRK2 promotes AICD activity and neurotoxicity in Parkinson's disease.
GO:0004674 protein serine/threonine kinase activity
IMP
PMID:28720718
Phosphorylation of amyloid precursor protein by mutant LRRK2...
ACCEPT
Summary: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Reason: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Supporting Evidence:
PMID:28720718
Phosphorylation of amyloid precursor protein by mutant LRRK2 promotes AICD activity and neurotoxicity in Parkinson's disease.
GO:0005515 protein binding
IPI
PMID:26014385
LRRK2 Promotes Tau Accumulation, Aggregation and Release.
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:26014385
2015 May 27. LRRK2 Promotes Tau Accumulation, Aggregation and Release.
GO:0005515 protein binding
IPI
PMID:28720718
Phosphorylation of amyloid precursor protein by mutant LRRK2...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:28720718
Phosphorylation of amyloid precursor protein by mutant LRRK2 promotes AICD activity and neurotoxicity in Parkinson's disease.
GO:0005737 cytoplasm
IMP
PMID:26014385
LRRK2 Promotes Tau Accumulation, Aggregation and Release.
ACCEPT
Summary: Cytoplasm is a major localization for LRRK2.
Reason: Cytoplasm is a major localization for LRRK2.
Supporting Evidence:
PMID:26014385
2015 May 27. LRRK2 Promotes Tau Accumulation, Aggregation and Release.
GO:0006468 protein phosphorylation
IMP
PMID:28720718
Phosphorylation of amyloid precursor protein by mutant LRRK2...
ACCEPT
Summary: Protein phosphorylation - core enzymatic function of LRRK2 kinase domain.
Reason: Protein phosphorylation - core enzymatic function of LRRK2 kinase domain.
Supporting Evidence:
PMID:28720718
Phosphorylation of amyloid precursor protein by mutant LRRK2 promotes AICD activity and neurotoxicity in Parkinson's disease.
GO:0031647 regulation of protein stability
IMP
PMID:26014385
LRRK2 Promotes Tau Accumulation, Aggregation and Release.
KEEP AS NON CORE
Summary: Regulation of protein stability - tau-related.
Reason: Regulation of protein stability - tau-related.
Supporting Evidence:
PMID:26014385
2015 May 27. LRRK2 Promotes Tau Accumulation, Aggregation and Release.
GO:0007029 endoplasmic reticulum organization
IMP
PMID:25201882
Leucine-rich repeat kinase 2 regulates Sec16A at ER exit sit...
ACCEPT
Summary: ER organization is related to LRRK2 role in Sec16A regulation at ER exit sites.
Reason: ER organization is related to LRRK2 role in Sec16A regulation at ER exit sites.
Supporting Evidence:
PMID:25201882
Sep 8. Leucine-rich repeat kinase 2 regulates Sec16A at ER exit sites to allow ER-Golgi export.
GO:0060628 regulation of ER to Golgi vesicle-mediated transport
ISS
GO_REF:0000024
ACCEPT
Summary: Regulation of ER to Golgi vesicle-mediated transport - via Sec16A regulation.
Reason: Regulation of ER to Golgi vesicle-mediated transport - via Sec16A regulation.
GO:0070971 endoplasmic reticulum exit site
IDA
PMID:25201882
Leucine-rich repeat kinase 2 regulates Sec16A at ER exit sit...
ACCEPT
Summary: ER exit site localization - LRRK2 regulates Sec16A at ER exit sites.
Reason: ER exit site localization - LRRK2 regulates Sec16A at ER exit sites.
Supporting Evidence:
PMID:25201882
Sep 8. Leucine-rich repeat kinase 2 regulates Sec16A at ER exit sites to allow ER-Golgi export.
GO:0070973 protein localization to endoplasmic reticulum exit site
IMP
PMID:25201882
Leucine-rich repeat kinase 2 regulates Sec16A at ER exit sit...
MODIFY
Summary: Protein localization to ER exit site - LRRK2 regulates Sec16A; the annotated term is now obsolete.
Reason: GO:0070973 protein localization to endoplasmic reticulum exit site was obsoleted (GO release 2026-07-26) because it conflated distinct processes; GO suggests context-specific replacements. PMID:25201882 shows LRRK2 anchors Sec16A at ER exit sites and that this regulates anterograde ER-to-Golgi export (LRRK2 depletion disperses Sec16A and impairs ER export), so the finding is best captured by GO:0060628 regulation of ER to Golgi vesicle-mediated transport.
Supporting Evidence:
PMID:25201882
LRRK2 regulates the anterograde ER-Golgi transport through anchoring Sec16A at the endoplasmic reticulum exit sites
GO:0099400 caveola neck
IDA
PMID:19640926
LRRK2 regulates autophagic activity and localizes to specifi...
KEEP AS NON CORE
Summary: Caveola neck - specific localization.
Reason: Caveola neck - specific localization.
Supporting Evidence:
PMID:19640926
Jul 29. LRRK2 regulates autophagic activity and localizes to specific membrane microdomains in a novel human genomic reporter cellular model.
GO:1905289 regulation of CAMKK-AMPK signaling cascade
IMP
PMID:22012985
Leucine-rich repeat kinase 2 regulates autophagy through a c...
KEEP AS NON CORE
Summary: Regulation of CAMKK-AMPK signaling cascade - downstream effect.
Reason: Regulation of CAMKK-AMPK signaling cascade - downstream effect.
Supporting Evidence:
PMID:22012985
Leucine-rich repeat kinase 2 regulates autophagy through a calcium-dependent pathway involving NAADP.
GO:0005794 Golgi apparatus
IDA
PMID:23395371
RAB7L1 interacts with LRRK2 to modify intraneuronal protein ...
ACCEPT
Summary: Golgi apparatus localization is supported - LRRK2 is recruited to Golgi by Rab29.
Reason: Golgi apparatus localization is supported - LRRK2 is recruited to Golgi by Rab29.
Supporting Evidence:
PMID:23395371
RAB7L1 interacts with LRRK2 to modify intraneuronal protein sorting and Parkinson's disease risk.
GO:1905279 regulation of retrograde transport, endosome to Golgi
IGI
PMID:23395371
RAB7L1 interacts with LRRK2 to modify intraneuronal protein ...
ACCEPT
Summary: Regulation of retrograde transport, endosome to Golgi - via Rab29/RAB7L1 interaction.
Reason: Regulation of retrograde transport, endosome to Golgi - via Rab29/RAB7L1 interaction.
Supporting Evidence:
PMID:23395371
RAB7L1 interacts with LRRK2 to modify intraneuronal protein sorting and Parkinson's disease risk.
GO:0060828 regulation of canonical Wnt signaling pathway
TAS
PMID:22988876
The importance of Wnt signalling for neurodegeneration in Pa...
KEEP AS NON CORE
Summary: Regulation of canonical Wnt signaling pathway - LRRK2 acts as scaffold but not core function.
Reason: Regulation of canonical Wnt signaling pathway - LRRK2 acts as scaffold but not core function.
Supporting Evidence:
PMID:22988876
The importance of Wnt signalling for neurodegeneration in Parkinson's disease.
GO:0008017 microtubule binding
TAS
PMID:24115276
The regulation and deregulation of Wnt signaling by PARK gen...
KEEP AS NON CORE
Summary: Microtubule binding - documented but significance unclear.
Reason: Microtubule binding - documented but significance unclear.
Supporting Evidence:
PMID:24115276
The regulation and deregulation of Wnt signaling by PARK genes in health and disease.
GO:0060070 canonical Wnt signaling pathway
TAS
PMID:24115276
The regulation and deregulation of Wnt signaling by PARK gen...
KEEP AS NON CORE
Summary: Canonical Wnt signaling pathway - scaffold role.
Reason: Canonical Wnt signaling pathway - scaffold role.
Supporting Evidence:
PMID:24115276
The regulation and deregulation of Wnt signaling by PARK genes in health and disease.
GO:1904713 beta-catenin destruction complex binding
NAS
PMID:24115276
The regulation and deregulation of Wnt signaling by PARK gen...
KEEP AS NON CORE
Summary: Beta-catenin destruction complex binding - scaffold role.
Reason: Beta-catenin destruction complex binding - scaffold role.
Supporting Evidence:
PMID:24115276
The regulation and deregulation of Wnt signaling by PARK genes in health and disease.
GO:1904887 Wnt signalosome assembly
TAS
PMID:24115276
The regulation and deregulation of Wnt signaling by PARK gen...
KEEP AS NON CORE
Summary: Wnt signalosome assembly - via LRP6 bridging.
Reason: Wnt signalosome assembly - via LRP6 bridging.
Supporting Evidence:
PMID:24115276
The regulation and deregulation of Wnt signaling by PARK genes in health and disease.
GO:1990909 Wnt signalosome
NAS
PMID:24115276
The regulation and deregulation of Wnt signaling by PARK gen...
KEEP AS NON CORE
Summary: Wnt signalosome localization - scaffold role.
Reason: Wnt signalosome localization - scaffold role.
Supporting Evidence:
PMID:24115276
The regulation and deregulation of Wnt signaling by PARK genes in health and disease.
GO:1904887 Wnt signalosome assembly
IPI
PMID:22899650
LRRK2 functions as a Wnt signaling scaffold, bridging cytoso...
KEEP AS NON CORE
Summary: Wnt signalosome assembly - via LRP6 bridging.
Reason: Wnt signalosome assembly - via LRP6 bridging.
Supporting Evidence:
PMID:22899650
Aug 16. LRRK2 functions as a Wnt signaling scaffold, bridging cytosolic proteins and membrane-localized LRP6.
GO:0035751 regulation of lysosomal lumen pH
IMP
PMID:22012985
Leucine-rich repeat kinase 2 regulates autophagy through a c...
ACCEPT
Summary: Regulation of lysosomal lumen pH - via effects on lysosomal function.
Reason: Regulation of lysosomal lumen pH - via effects on lysosomal function.
Supporting Evidence:
PMID:22012985
Leucine-rich repeat kinase 2 regulates autophagy through a calcium-dependent pathway involving NAADP.
GO:1990909 Wnt signalosome
IDA
PMID:22899650
LRRK2 functions as a Wnt signaling scaffold, bridging cytoso...
KEEP AS NON CORE
Summary: Wnt signalosome localization - scaffold role.
Reason: Wnt signalosome localization - scaffold role.
Supporting Evidence:
PMID:22899650
Aug 16. LRRK2 functions as a Wnt signaling scaffold, bridging cytosolic proteins and membrane-localized LRP6.
GO:0005783 endoplasmic reticulum
IDA
PMID:19640926
LRRK2 regulates autophagic activity and localizes to specifi...
ACCEPT
Summary: Endoplasmic reticulum - documented localization at ER exit sites.
Reason: Endoplasmic reticulum - documented localization at ER exit sites.
Supporting Evidence:
PMID:19640926
Jul 29. LRRK2 regulates autophagic activity and localizes to specific membrane microdomains in a novel human genomic reporter cellular model.
GO:0005902 microvillus
IDA
PMID:19640926
LRRK2 regulates autophagic activity and localizes to specifi...
KEEP AS NON CORE
Summary: Microvillus - specific localization.
Reason: Microvillus - specific localization.
Supporting Evidence:
PMID:19640926
Jul 29. LRRK2 regulates autophagic activity and localizes to specific membrane microdomains in a novel human genomic reporter cellular model.
GO:0016242 negative regulation of macroautophagy
IMP
PMID:19640926
LRRK2 regulates autophagic activity and localizes to specifi...
MARK AS OVER ANNOTATED
Summary: Negative regulation of macroautophagy is downstream of trafficking effects.
Reason: Negative regulation of macroautophagy is downstream of trafficking effects.
Supporting Evidence:
PMID:19640926
Jul 29. LRRK2 regulates autophagic activity and localizes to specific membrane microdomains in a novel human genomic reporter cellular model.
GO:0044753 amphisome
IDA
PMID:19640926
LRRK2 regulates autophagic activity and localizes to specifi...
KEEP AS NON CORE
Summary: Amphisome - autophagy-related.
Reason: Amphisome - autophagy-related.
Supporting Evidence:
PMID:19640926
Jul 29. LRRK2 regulates autophagic activity and localizes to specific membrane microdomains in a novel human genomic reporter cellular model.
GO:0044754 autolysosome
IDA
PMID:19640926
LRRK2 regulates autophagic activity and localizes to specifi...
KEEP AS NON CORE
Summary: Autolysosome - autophagy-related.
Reason: Autolysosome - autophagy-related.
Supporting Evidence:
PMID:19640926
Jul 29. LRRK2 regulates autophagic activity and localizes to specific membrane microdomains in a novel human genomic reporter cellular model.
GO:0097487 multivesicular body, internal vesicle
IDA
PMID:19640926
LRRK2 regulates autophagic activity and localizes to specifi...
KEEP AS NON CORE
Summary: Multivesicular body, internal vesicle - endosomal.
Reason: Multivesicular body, internal vesicle - endosomal.
Supporting Evidence:
PMID:19640926
Jul 29. LRRK2 regulates autophagic activity and localizes to specific membrane microdomains in a novel human genomic reporter cellular model.
GO:0005886 plasma membrane
IDA
PMID:22899650
LRRK2 functions as a Wnt signaling scaffold, bridging cytoso...
KEEP AS NON CORE
Summary: Plasma membrane - LRRK2 can localize to membranes but cytosol is predominant.
Reason: Plasma membrane - LRRK2 can localize to membranes but cytosol is predominant.
Supporting Evidence:
PMID:22899650
Aug 16. LRRK2 functions as a Wnt signaling scaffold, bridging cytosolic proteins and membrane-localized LRP6.
GO:0016301 kinase activity
IMP
PMID:23916833
Inhibition of LRRK2 kinase activity stimulates macroautophag...
ACCEPT
Summary: Kinase activity is a core function - LRRK2 is a dual-function kinase/GTPase.
Reason: Kinase activity is a core function - LRRK2 is a dual-function kinase/GTPase.
Supporting Evidence:
PMID:23916833
Inhibition of LRRK2 kinase activity stimulates macroautophagy.
GO:1902902 negative regulation of autophagosome assembly
IMP
PMID:23916833
Inhibition of LRRK2 kinase activity stimulates macroautophag...
MARK AS OVER ANNOTATED
Summary: Negative regulation of autophagosome assembly is downstream effect.
Reason: Negative regulation of autophagosome assembly is downstream effect.
Supporting Evidence:
PMID:23916833
Inhibition of LRRK2 kinase activity stimulates macroautophagy.
GO:0009267 cellular response to starvation
IMP
PMID:24211199
Pathogenic Parkinson's disease mutations across the function...
MARK AS OVER ANNOTATED
Summary: Cellular response to starvation is experimental condition.
Reason: Cellular response to starvation is experimental condition.
Supporting Evidence:
PMID:24211199
Pathogenic Parkinson's disease mutations across the functional domains of LRRK2 alter the autophagic/lysosomal response to starvation.
GO:0010506 regulation of autophagy
IMP
PMID:24211199
Pathogenic Parkinson's disease mutations across the function...
MARK AS OVER ANNOTATED
Summary: Regulation of autophagy is downstream of trafficking/lysosomal effects.
Reason: Regulation of autophagy is downstream of trafficking/lysosomal effects.
Supporting Evidence:
PMID:24211199
Pathogenic Parkinson's disease mutations across the functional domains of LRRK2 alter the autophagic/lysosomal response to starvation.
GO:0004674 protein serine/threonine kinase activity
TAS
PMID:22899650
LRRK2 functions as a Wnt signaling scaffold, bridging cytoso...
ACCEPT
Summary: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Reason: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Supporting Evidence:
PMID:22899650
Aug 16. LRRK2 functions as a Wnt signaling scaffold, bridging cytosolic proteins and membrane-localized LRP6.
GO:0005525 GTP binding
TAS
PMID:22899650
LRRK2 functions as a Wnt signaling scaffold, bridging cytoso...
ACCEPT
Summary: GTP binding is essential for LRRK2 ROC domain function and regulates kinase activity.
Reason: GTP binding is essential for LRRK2 ROC domain function and regulates kinase activity.
Supporting Evidence:
PMID:22899650
Aug 16. LRRK2 functions as a Wnt signaling scaffold, bridging cytosolic proteins and membrane-localized LRP6.
GO:0005829 cytosol
IDA
PMID:22899650
LRRK2 functions as a Wnt signaling scaffold, bridging cytoso...
ACCEPT
Summary: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
Reason: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
Supporting Evidence:
PMID:22899650
Aug 16. LRRK2 functions as a Wnt signaling scaffold, bridging cytosolic proteins and membrane-localized LRP6.
GO:0004672 protein kinase activity
IDA
PMID:25500533
Phosphorylation of LRRK2 by casein kinase 1Ξ± regulates trans...
ACCEPT
Summary: Protein kinase activity is a core function of LRRK2, phosphorylating Rab GTPases and other substrates.
Reason: Protein kinase activity is a core function of LRRK2, phosphorylating Rab GTPases and other substrates.
Supporting Evidence:
PMID:25500533
Phosphorylation of LRRK2 by casein kinase 1Ξ± regulates trans-Golgi clustering via differential interaction with ARHGEF7.
GO:0005798 Golgi-associated vesicle
IDA
PMID:24510904
Unbiased screen for interactors of leucine-rich repeat kinas...
ACCEPT
Summary: Golgi-associated vesicle - consistent with trafficking role.
Reason: Golgi-associated vesicle - consistent with trafficking role.
Supporting Evidence:
PMID:24510904
Unbiased screen for interactors of leucine-rich repeat kinase 2 supports a common pathway for sporadic and familial Parkinson disease.
GO:0007030 Golgi organization
IMP
PMID:24510904
Unbiased screen for interactors of leucine-rich repeat kinas...
ACCEPT
Summary: Golgi organization - LRRK2 regulates Golgi-related trafficking through Rab phosphorylation.
Reason: Golgi organization - LRRK2 regulates Golgi-related trafficking through Rab phosphorylation.
Supporting Evidence:
PMID:24510904
Unbiased screen for interactors of leucine-rich repeat kinase 2 supports a common pathway for sporadic and familial Parkinson disease.
GO:0005737 cytoplasm
IDA
PMID:19625296
Mutations in the LRRK2 Roc-COR tandem domain link Parkinson'...
ACCEPT
Summary: Cytoplasm is a major localization for LRRK2.
Reason: Cytoplasm is a major localization for LRRK2.
Supporting Evidence:
PMID:19625296
Jul 22. Mutations in the LRRK2 Roc-COR tandem domain link Parkinson's disease to Wnt signalling pathways.
GO:0030426 growth cone
IDA
PMID:19625296
Mutations in the LRRK2 Roc-COR tandem domain link Parkinson'...
KEEP AS NON CORE
Summary: Growth cone is tissue-specific neuronal structure.
Reason: Growth cone is tissue-specific neuronal structure.
Supporting Evidence:
PMID:19625296
Jul 22. Mutations in the LRRK2 Roc-COR tandem domain link Parkinson's disease to Wnt signalling pathways.
GO:0043005 neuron projection
IDA
PMID:19625296
Mutations in the LRRK2 Roc-COR tandem domain link Parkinson'...
KEEP AS NON CORE
Summary: Neuron projection is tissue-specific in neurons.
Reason: Neuron projection is tissue-specific in neurons.
Supporting Evidence:
PMID:19625296
Jul 22. Mutations in the LRRK2 Roc-COR tandem domain link Parkinson's disease to Wnt signalling pathways.
GO:0090394 negative regulation of excitatory postsynaptic potential
ISS
GO_REF:0000024
KEEP AS NON CORE
Summary: Negative regulation of excitatory postsynaptic potential - tissue-specific.
Reason: Negative regulation of excitatory postsynaptic potential - tissue-specific.
GO:0007040 lysosome organization
IMP
PMID:25416817
Dysregulation of lysosomal morphology by pathogenic LRRK2 is...
ACCEPT
Summary: Lysosome organization - consistent with endolysosomal trafficking role.
Reason: Lysosome organization - consistent with endolysosomal trafficking role.
Supporting Evidence:
PMID:25416817
Dysregulation of lysosomal morphology by pathogenic LRRK2 is corrected by TPC2 inhibition.
GO:0019722 calcium-mediated signaling
IMP
PMID:25416817
Dysregulation of lysosomal morphology by pathogenic LRRK2 is...
KEEP AS NON CORE
Summary: Calcium-mediated signaling - via NAADP pathway.
Reason: Calcium-mediated signaling - via NAADP pathway.
Supporting Evidence:
PMID:25416817
Dysregulation of lysosomal morphology by pathogenic LRRK2 is corrected by TPC2 inhibition.
GO:0046039 GTP metabolic process
IDA
PMID:21048939
ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange facto...
ACCEPT
Summary: GTP metabolic process - related to GTPase activity.
Reason: GTP metabolic process - related to GTPase activity.
Supporting Evidence:
PMID:21048939
ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange factor for leucine-rich repeat kinase 2.
GO:0007005 mitochondrion organization
IMP
PMID:22764206
Pharmacological rescue of mitochondrial deficits in iPSC-der...
MARK AS OVER ANNOTATED
Summary: Mitochondrion organization effects are likely secondary.
Reason: Mitochondrion organization effects are likely secondary.
Supporting Evidence:
PMID:22764206
Pharmacological rescue of mitochondrial deficits in iPSC-derived neural cells from patients with familial Parkinson's disease.
GO:0034599 cellular response to oxidative stress
IMP
PMID:22764206
Pharmacological rescue of mitochondrial deficits in iPSC-der...
MARK AS OVER ANNOTATED
Summary: Cellular response to oxidative stress is a pleiotropic disease-related effect.
Reason: Cellular response to oxidative stress is a pleiotropic disease-related effect.
Supporting Evidence:
PMID:22764206
Pharmacological rescue of mitochondrial deficits in iPSC-derived neural cells from patients with familial Parkinson's disease.
GO:0051646 mitochondrion localization
IMP
PMID:22764206
Pharmacological rescue of mitochondrial deficits in iPSC-der...
MARK AS OVER ANNOTATED
Summary: Mitochondrion localization effects are secondary.
Reason: Mitochondrion localization effects are secondary.
Supporting Evidence:
PMID:22764206
Pharmacological rescue of mitochondrial deficits in iPSC-derived neural cells from patients with familial Parkinson's disease.
GO:0005829 cytosol
TAS
Reactome:R-HSA-8857565
ACCEPT
Summary: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
Reason: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
GO:0005829 cytosol
TAS
Reactome:R-HSA-8857577
ACCEPT
Summary: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
Reason: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
GO:0005829 cytosol
TAS
Reactome:R-HSA-8857583
ACCEPT
Summary: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
Reason: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
GO:0005829 cytosol
TAS
Reactome:R-HSA-9634702
ACCEPT
Summary: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
Reason: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
GO:0034599 cellular response to oxidative stress
IMP
PMID:21857923
Dysregulated LRRK2 signaling in response to endoplasmic reti...
MARK AS OVER ANNOTATED
Summary: Cellular response to oxidative stress is a pleiotropic disease-related effect.
Reason: Cellular response to oxidative stress is a pleiotropic disease-related effect.
Supporting Evidence:
PMID:21857923
Dysregulated LRRK2 signaling in response to endoplasmic reticulum stress leads to dopaminergic neuron degeneration in C.
GO:1902236 negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
IMP
PMID:21857923
Dysregulated LRRK2 signaling in response to endoplasmic reti...
MARK AS OVER ANNOTATED
Summary: Negative regulation of ER stress-induced intrinsic apoptotic signaling pathway is disease-related.
Reason: Negative regulation of ER stress-induced intrinsic apoptotic signaling pathway is disease-related.
Supporting Evidence:
PMID:21857923
Dysregulated LRRK2 signaling in response to endoplasmic reticulum stress leads to dopaminergic neuron degeneration in C.
GO:0014041 regulation of neuron maturation
IMP
PMID:21168496
Adult neurogenesis and neurite outgrowth are impaired in LRR...
MARK AS OVER ANNOTATED
Summary: Regulation of neuron maturation is developmental.
Reason: Regulation of neuron maturation is developmental.
Supporting Evidence:
PMID:21168496
2010 Dec 16. Adult neurogenesis and neurite outgrowth are impaired in LRRK2 G2019S mice.
GO:0021772 olfactory bulb development
IMP
PMID:21168496
Adult neurogenesis and neurite outgrowth are impaired in LRR...
MARK AS OVER ANNOTATED
Summary: Olfactory bulb development is tissue-specific.
Reason: Olfactory bulb development is tissue-specific.
Supporting Evidence:
PMID:21168496
2010 Dec 16. Adult neurogenesis and neurite outgrowth are impaired in LRRK2 G2019S mice.
GO:0022028 tangential migration from the subventricular zone to the olfactory bulb
IMP
PMID:21168496
Adult neurogenesis and neurite outgrowth are impaired in LRR...
MARK AS OVER ANNOTATED
Summary: Tangential migration from subventricular zone is developmental.
Reason: Tangential migration from subventricular zone is developmental.
Supporting Evidence:
PMID:21168496
2010 Dec 16. Adult neurogenesis and neurite outgrowth are impaired in LRRK2 G2019S mice.
GO:0061001 regulation of dendritic spine morphogenesis
IMP
PMID:21168496
Adult neurogenesis and neurite outgrowth are impaired in LRR...
KEEP AS NON CORE
Summary: Regulation of dendritic spine morphogenesis - neuronal effect.
Reason: Regulation of dendritic spine morphogenesis - neuronal effect.
Supporting Evidence:
PMID:21168496
2010 Dec 16. Adult neurogenesis and neurite outgrowth are impaired in LRRK2 G2019S mice.
GO:2000172 regulation of branching morphogenesis of a nerve
IMP
PMID:21168496
Adult neurogenesis and neurite outgrowth are impaired in LRR...
MARK AS OVER ANNOTATED
Summary: Regulation of branching morphogenesis of a nerve is developmental.
Reason: Regulation of branching morphogenesis of a nerve is developmental.
Supporting Evidence:
PMID:21168496
2010 Dec 16. Adult neurogenesis and neurite outgrowth are impaired in LRRK2 G2019S mice.
GO:0061001 regulation of dendritic spine morphogenesis
ISS
GO_REF:0000024
KEEP AS NON CORE
Summary: Regulation of dendritic spine morphogenesis - neuronal effect.
Reason: Regulation of dendritic spine morphogenesis - neuronal effect.
GO:0070585 protein localization to mitochondrion
TAS
PMID:24252804
The role of oxidative stress in Parkinson's disease.
MARK AS OVER ANNOTATED
Summary: Protein localization to mitochondrion is secondary.
Reason: Protein localization to mitochondrion is secondary.
Supporting Evidence:
PMID:24252804
The role of oxidative stress in Parkinson's disease.
GO:0090140 regulation of mitochondrial fission
TAS
PMID:24252804
The role of oxidative stress in Parkinson's disease.
MARK AS OVER ANNOTATED
Summary: Regulation of mitochondrial fission is secondary effect.
Reason: Regulation of mitochondrial fission is secondary effect.
Supporting Evidence:
PMID:24252804
The role of oxidative stress in Parkinson's disease.
GO:0043195 terminal bouton
TAS
PMID:21563316
Synaptic vesicle trafficking and Parkinson's disease.
KEEP AS NON CORE
Summary: Terminal bouton is tissue-specific neuronal localization.
Reason: Terminal bouton is tissue-specific neuronal localization.
Supporting Evidence:
PMID:21563316
Synaptic vesicle trafficking and Parkinson's disease.
GO:0071287 cellular response to manganese ion
IMP
PMID:23628791
Down-regulation of LRRK2 in control and DAT transfected HEK ...
MARK AS OVER ANNOTATED
Summary: Cellular response to manganese ion is experimental.
Reason: Cellular response to manganese ion is experimental.
Supporting Evidence:
PMID:23628791
2013 Apr 27. Down-regulation of LRRK2 in control and DAT transfected HEK cells increases manganese-induced oxidative stress and cell toxicity.
GO:1902803 regulation of synaptic vesicle transport
TAS
PMID:21563316
Synaptic vesicle trafficking and Parkinson's disease.
KEEP AS NON CORE
Summary: Regulation of synaptic vesicle transport - tissue-specific.
Reason: Regulation of synaptic vesicle transport - tissue-specific.
Supporting Evidence:
PMID:21563316
Synaptic vesicle trafficking and Parkinson's disease.
GO:1903351 cellular response to dopamine
IMP
PMID:23628791
Down-regulation of LRRK2 in control and DAT transfected HEK ...
MARK AS OVER ANNOTATED
Summary: Cellular response to dopamine is tissue-specific.
Reason: Cellular response to dopamine is tissue-specific.
Supporting Evidence:
PMID:23628791
2013 Apr 27. Down-regulation of LRRK2 in control and DAT transfected HEK cells increases manganese-induced oxidative stress and cell toxicity.
GO:0006897 endocytosis
IMP
PMID:24576675
LRRK2, but not pathogenic mutants, protects against H2O2 str...
KEEP AS NON CORE
Summary: Endocytosis - related to synaptic vesicle endocytosis role.
Reason: Endocytosis - related to synaptic vesicle endocytosis role.
Supporting Evidence:
PMID:24576675
LRRK2, but not pathogenic mutants, protects against H2O2 stress depending on mitochondrial function and endocytosis in a yeast model.
GO:0006897 endocytosis
IGI
PMID:24576675
LRRK2, but not pathogenic mutants, protects against H2O2 str...
KEEP AS NON CORE
Summary: Endocytosis - related to synaptic vesicle endocytosis role.
Reason: Endocytosis - related to synaptic vesicle endocytosis role.
Supporting Evidence:
PMID:24576675
LRRK2, but not pathogenic mutants, protects against H2O2 stress depending on mitochondrial function and endocytosis in a yeast model.
GO:0034599 cellular response to oxidative stress
IMP
PMID:21362567
LRRK2 mutant iPSC-derived DA neurons demonstrate increased s...
MARK AS OVER ANNOTATED
Summary: Cellular response to oxidative stress is a pleiotropic disease-related effect.
Reason: Cellular response to oxidative stress is a pleiotropic disease-related effect.
Supporting Evidence:
PMID:21362567
LRRK2 mutant iPSC-derived DA neurons demonstrate increased susceptibility to oxidative stress.
GO:0036479 peroxidase inhibitor activity
IDA
PMID:21850687
Mutations in LRRK2 increase phosphorylation of peroxiredoxin...
UNDECIDED
Summary: Peroxidase inhibitor activity - reported but unclear if core function.
Reason: Peroxidase inhibitor activity - reported but unclear if core function.
Supporting Evidence:
PMID:21850687
Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death.
GO:1903748 negative regulation of protein localization to mitochondrion
IDA
PMID:21370995
Expression of leucine-rich repeat kinase 2 (LRRK2) inhibits ...
UNDECIDED
Summary: GO term GO:1903748 requires further review - no pre-defined decision available.
Reason: Term not in curated decision list, requires manual review.
Supporting Evidence:
PMID:21370995
Expression of leucine-rich repeat kinase 2 (LRRK2) inhibits the processing of uMtCK to induce cell death in a cell culture model system.
GO:1902499 positive regulation of protein autoubiquitination
IDA
PMID:16352719
Leucine-rich repeat kinase 2 (LRRK2) interacts with parkin, ...
KEEP AS NON CORE
Summary: Positive regulation of protein autoubiquitination - parkin-related.
Reason: Positive regulation of protein autoubiquitination - parkin-related.
Supporting Evidence:
PMID:16352719
Leucine-rich repeat kinase 2 (LRRK2) interacts with parkin, and mutant LRRK2 induces neuronal degeneration.
GO:0044325 transmembrane transporter binding
IPI
PMID:21370995
Expression of leucine-rich repeat kinase 2 (LRRK2) inhibits ...
KEEP AS NON CORE
Summary: Transmembrane transporter binding - various interactions.
Reason: Transmembrane transporter binding - various interactions.
Supporting Evidence:
PMID:21370995
Expression of leucine-rich repeat kinase 2 (LRRK2) inhibits the processing of uMtCK to induce cell death in a cell culture model system.
GO:0010955 negative regulation of protein processing
IDA
PMID:21370995
Expression of leucine-rich repeat kinase 2 (LRRK2) inhibits ...
KEEP AS NON CORE
Summary: Negative regulation of protein processing - specific effect.
Reason: Negative regulation of protein processing - specific effect.
Supporting Evidence:
PMID:21370995
Expression of leucine-rich repeat kinase 2 (LRRK2) inhibits the processing of uMtCK to induce cell death in a cell culture model system.
GO:1903748 negative regulation of protein localization to mitochondrion
IC
PMID:21370995
Expression of leucine-rich repeat kinase 2 (LRRK2) inhibits ...
UNDECIDED
Summary: GO term GO:1903748 requires further review - no pre-defined decision available.
Reason: Term not in curated decision list, requires manual review.
Supporting Evidence:
PMID:21370995
Expression of leucine-rich repeat kinase 2 (LRRK2) inhibits the processing of uMtCK to induce cell death in a cell culture model system.
GO:0008104 intracellular protein localization
ISS
GO_REF:0000024
ACCEPT
Summary: Intracellular protein localization - broad but relevant to trafficking role.
Reason: Intracellular protein localization - broad but relevant to trafficking role.
GO:0031398 positive regulation of protein ubiquitination
IDA
PMID:20173330
LRRK2 and the stress response: interaction with MKKs and JNK...
KEEP AS NON CORE
Summary: Positive regulation of protein ubiquitination - related to parkin interaction.
Reason: Positive regulation of protein ubiquitination - related to parkin interaction.
Supporting Evidence:
PMID:20173330
LRRK2 and the stress response: interaction with MKKs and JNK-interacting proteins.
GO:0035556 intracellular signal transduction
ISS
GO_REF:0000024
ACCEPT
Summary: Intracellular signal transduction - LRRK2 functions as a kinase that phosphorylates Rab GTPases.
Reason: Intracellular signal transduction - LRRK2 functions as a kinase that phosphorylates Rab GTPases.
GO:0051966 regulation of synaptic transmission, glutamatergic
ISS
GO_REF:0000024
KEEP AS NON CORE
Summary: Regulation of synaptic transmission, glutamatergic - tissue-specific.
Reason: Regulation of synaptic transmission, glutamatergic - tissue-specific.
GO:0060079 excitatory postsynaptic potential
ISS
GO_REF:0000024
KEEP AS NON CORE
Summary: Excitatory postsynaptic potential - tissue-specific.
Reason: Excitatory postsynaptic potential - tissue-specific.
GO:0060159 regulation of dopamine receptor signaling pathway
ISS
GO_REF:0000024
KEEP AS NON CORE
Summary: Regulation of dopamine receptor signaling pathway - tissue-specific.
Reason: Regulation of dopamine receptor signaling pathway - tissue-specific.
GO:0004674 protein serine/threonine kinase activity
IDA
PMID:21850687
Mutations in LRRK2 increase phosphorylation of peroxiredoxin...
ACCEPT
Summary: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Reason: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Supporting Evidence:
PMID:21850687
Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death.
GO:0005829 cytosol
IDA
PMID:21850687
Mutations in LRRK2 increase phosphorylation of peroxiredoxin...
ACCEPT
Summary: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
Reason: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
Supporting Evidence:
PMID:21850687
Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death.
GO:0005741 mitochondrial outer membrane
ISS
GO_REF:0000024
KEEP AS NON CORE
Summary: Mitochondrial outer membrane - reported but may be minor.
Reason: Mitochondrial outer membrane - reported but may be minor.
GO:0005743 mitochondrial inner membrane
ISS
GO_REF:0000024
KEEP AS NON CORE
Summary: Mitochondrial inner membrane - reported but may be minor.
Reason: Mitochondrial inner membrane - reported but may be minor.
GO:0005759 mitochondrial matrix
ISS
GO_REF:0000024
KEEP AS NON CORE
Summary: Mitochondrial matrix - reported but may be minor.
Reason: Mitochondrial matrix - reported but may be minor.
GO:0005764 lysosome
ISS
GO_REF:0000024
ACCEPT
Summary: Lysosome localization is consistent with LRRK2 role in endolysosomal trafficking.
Reason: Lysosome localization is consistent with LRRK2 role in endolysosomal trafficking.
GO:0005768 endosome
ISS
GO_REF:0000024
ACCEPT
Summary: Endosome localization is consistent with LRRK2 role in regulating Rab-dependent trafficking.
Reason: Endosome localization is consistent with LRRK2 role in regulating Rab-dependent trafficking.
GO:0005783 endoplasmic reticulum
ISS
GO_REF:0000024
ACCEPT
Summary: Endoplasmic reticulum - documented localization at ER exit sites.
Reason: Endoplasmic reticulum - documented localization at ER exit sites.
GO:0005794 Golgi apparatus
ISS
GO_REF:0000024
ACCEPT
Summary: Golgi apparatus localization is supported - LRRK2 is recruited to Golgi by Rab29.
Reason: Golgi apparatus localization is supported - LRRK2 is recruited to Golgi by Rab29.
IDA
PMID:17120249
Localization of LRRK2 to membranous and vesicular structures...
KEEP AS NON CORE
Summary: Axon localization is tissue-specific in neurons.
Reason: Axon localization is tissue-specific in neurons.
Supporting Evidence:
PMID:17120249
Localization of LRRK2 to membranous and vesicular structures in mammalian brain.
GO:0030425 dendrite
IDA
PMID:17120249
Localization of LRRK2 to membranous and vesicular structures...
KEEP AS NON CORE
Summary: Dendrite localization is tissue-specific in neurons, not a core localization.
Reason: Dendrite localization is tissue-specific in neurons, not a core localization.
Supporting Evidence:
PMID:17120249
Localization of LRRK2 to membranous and vesicular structures in mammalian brain.
GO:0031410 cytoplasmic vesicle
ISS
GO_REF:0000024
ACCEPT
Summary: Cytoplasmic vesicle - consistent with trafficking role.
Reason: Cytoplasmic vesicle - consistent with trafficking role.
GO:0043204 perikaryon
IDA
PMID:17120249
Localization of LRRK2 to membranous and vesicular structures...
KEEP AS NON CORE
Summary: Perikaryon (neuronal cell body) is tissue-specific localization.
Reason: Perikaryon (neuronal cell body) is tissue-specific localization.
Supporting Evidence:
PMID:17120249
Localization of LRRK2 to membranous and vesicular structures in mammalian brain.
GO:0016301 kinase activity
IDA
PMID:17114044
The familial Parkinsonism gene LRRK2 regulates neurite proce...
ACCEPT
Summary: Kinase activity is a core function - LRRK2 is a dual-function kinase/GTPase.
Reason: Kinase activity is a core function - LRRK2 is a dual-function kinase/GTPase.
Supporting Evidence:
PMID:17114044
The familial Parkinsonism gene LRRK2 regulates neurite process morphology.
ISS
GO_REF:0000024
KEEP AS NON CORE
Summary: Axon localization is tissue-specific in neurons.
Reason: Axon localization is tissue-specific in neurons.
GO:0030425 dendrite
ISS
GO_REF:0000024
KEEP AS NON CORE
Summary: Dendrite localization is tissue-specific in neurons, not a core localization.
Reason: Dendrite localization is tissue-specific in neurons, not a core localization.
GO:0043204 perikaryon
ISS
GO_REF:0000024
KEEP AS NON CORE
Summary: Perikaryon (neuronal cell body) is tissue-specific localization.
Reason: Perikaryon (neuronal cell body) is tissue-specific localization.
GO:0048812 neuron projection morphogenesis
IMP
PMID:17114044
The familial Parkinsonism gene LRRK2 regulates neurite proce...
MARK AS OVER ANNOTATED
Summary: Neuron projection morphogenesis is developmental.
Reason: Neuron projection morphogenesis is developmental.
Supporting Evidence:
PMID:17114044
The familial Parkinsonism gene LRRK2 regulates neurite process morphology.
GO:0000149 SNARE binding
IPI
PMID:21307259
LRRK2 controls synaptic vesicle storage and mobilization wit...
KEEP AS NON CORE
Summary: SNARE binding - related to vesicle function.
Reason: SNARE binding - related to vesicle function.
Supporting Evidence:
PMID:21307259
LRRK2 controls synaptic vesicle storage and mobilization within the recycling pool.
GO:0003779 actin binding
IPI
PMID:21307259
LRRK2 controls synaptic vesicle storage and mobilization wit...
KEEP AS NON CORE
Summary: Actin binding - documented interaction.
Reason: Actin binding - documented interaction.
Supporting Evidence:
PMID:21307259
LRRK2 controls synaptic vesicle storage and mobilization within the recycling pool.
GO:0017075 syntaxin-1 binding
IPI
PMID:21307259
LRRK2 controls synaptic vesicle storage and mobilization wit...
KEEP AS NON CORE
Summary: Syntaxin-1 binding - vesicle-related.
Reason: Syntaxin-1 binding - vesicle-related.
Supporting Evidence:
PMID:21307259
LRRK2 controls synaptic vesicle storage and mobilization within the recycling pool.
GO:0030276 clathrin binding
IPI
PMID:21307259
LRRK2 controls synaptic vesicle storage and mobilization wit...
KEEP AS NON CORE
Summary: Clathrin binding - endocytosis-related.
Reason: Clathrin binding - endocytosis-related.
Supporting Evidence:
PMID:21307259
LRRK2 controls synaptic vesicle storage and mobilization within the recycling pool.
GO:1902803 regulation of synaptic vesicle transport
ISS
GO_REF:0000024
KEEP AS NON CORE
Summary: Regulation of synaptic vesicle transport - tissue-specific.
Reason: Regulation of synaptic vesicle transport - tissue-specific.
GO:1902823 negative regulation of late endosome to lysosome transport
TAS
PMID:23949442
LRRK2 phosphorylates Snapin and inhibits interaction of Snap...
KEEP AS NON CORE
Summary: Negative regulation of late endosome to lysosome transport - downstream effect.
Reason: Negative regulation of late endosome to lysosome transport - downstream effect.
Supporting Evidence:
PMID:23949442
LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25.
GO:0004674 protein serine/threonine kinase activity
IDA
PMID:23949442
LRRK2 phosphorylates Snapin and inhibits interaction of Snap...
ACCEPT
Summary: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Reason: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Supporting Evidence:
PMID:23949442
LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25.
GO:2000300 regulation of synaptic vesicle exocytosis
IMP
PMID:23949442
LRRK2 phosphorylates Snapin and inhibits interaction of Snap...
KEEP AS NON CORE
Summary: Regulation of synaptic vesicle exocytosis - tissue-specific.
Reason: Regulation of synaptic vesicle exocytosis - tissue-specific.
Supporting Evidence:
PMID:23949442
LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25.
GO:0051900 regulation of mitochondrial depolarization
IMP
PMID:22736029
G2019S leucine-rich repeat kinase 2 causes uncoupling protei...
MARK AS OVER ANNOTATED
Summary: Regulation of mitochondrial depolarization is disease-related.
Reason: Regulation of mitochondrial depolarization is disease-related.
Supporting Evidence:
PMID:22736029
Jun 26. G2019S leucine-rich repeat kinase 2 causes uncoupling protein-mediated mitochondrial depolarization.
GO:0005829 cytosol
IDA
PMID:24403142
Mutant LRRK2 toxicity in neurons depends on LRRK2 levels and...
ACCEPT
Summary: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
Reason: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
Supporting Evidence:
PMID:24403142
Mutant LRRK2 toxicity in neurons depends on LRRK2 levels and synuclein but not kinase activity or inclusion bodies.
GO:0051018 protein kinase A binding
IPI
PMID:24464040
LRRK2 regulates synaptogenesis and dopamine receptor activat...
KEEP AS NON CORE
Summary: Protein kinase A binding - interaction documented but not core.
Reason: Protein kinase A binding - interaction documented but not core.
Supporting Evidence:
PMID:24464040
LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity.
GO:0005829 cytosol
IDA
PMID:22736029
G2019S leucine-rich repeat kinase 2 causes uncoupling protei...
ACCEPT
Summary: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
Reason: Cytosol is the predominant localization of LRRK2, from which it is recruited to membranes.
Supporting Evidence:
PMID:22736029
Jun 26. G2019S leucine-rich repeat kinase 2 causes uncoupling protein-mediated mitochondrial depolarization.
GO:0031966 mitochondrial membrane
IDA
PMID:22736029
G2019S leucine-rich repeat kinase 2 causes uncoupling protei...
KEEP AS NON CORE
Summary: Mitochondrial membrane - reported.
Reason: Mitochondrial membrane - reported.
Supporting Evidence:
PMID:22736029
Jun 26. G2019S leucine-rich repeat kinase 2 causes uncoupling protein-mediated mitochondrial depolarization.
GO:1902692 regulation of neuroblast proliferation
IMP
PMID:21168496
Adult neurogenesis and neurite outgrowth are impaired in LRR...
MARK AS OVER ANNOTATED
Summary: Regulation of neuroblast proliferation is developmental.
Reason: Regulation of neuroblast proliferation is developmental.
Supporting Evidence:
PMID:21168496
2010 Dec 16. Adult neurogenesis and neurite outgrowth are impaired in LRRK2 G2019S mice.
GO:0005615 extracellular space
HDA
PMID:22664934
Comparison of tear protein levels in breast cancer patients ...
KEEP AS NON CORE
Summary: Extracellular space - exosome secretion.
Reason: Extracellular space - exosome secretion.
Supporting Evidence:
PMID:22664934
Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
GO:0070062 extracellular exosome
HDA
PMID:19056867
Large-scale proteomics and phosphoproteomics of urinary exos...
KEEP AS NON CORE
Summary: Extracellular exosome - secreted in exosomes.
Reason: Extracellular exosome - secreted in exosomes.
Supporting Evidence:
PMID:19056867
2008 Dec 3. Large-scale proteomics and phosphoproteomics of urinary exosomes.
GO:0032839 dendrite cytoplasm
IDA
PMID:21696411
LRRK2 expression in idiopathic and G2019S positive Parkinson...
KEEP AS NON CORE
Summary: Dendrite cytoplasm is tissue-specific.
Reason: Dendrite cytoplasm is tissue-specific.
Supporting Evidence:
PMID:21696411
LRRK2 expression in idiopathic and G2019S positive Parkinson's disease subjects: a morphological and quantitative study.
GO:0043025 neuronal cell body
IDA
PMID:21696411
LRRK2 expression in idiopathic and G2019S positive Parkinson...
KEEP AS NON CORE
Summary: Neuronal cell body is tissue-specific localization.
Reason: Neuronal cell body is tissue-specific localization.
Supporting Evidence:
PMID:21696411
LRRK2 expression in idiopathic and G2019S positive Parkinson's disease subjects: a morphological and quantitative study.
GO:0004674 protein serine/threonine kinase activity
IDA
PMID:22423108
ArfGAP1 is a GTPase activating protein for LRRK2: reciprocal...
ACCEPT
Summary: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Reason: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Supporting Evidence:
PMID:22423108
ArfGAP1 is a GTPase activating protein for LRRK2: reciprocal regulation of ArfGAP1 by LRRK2.
GO:0034260 negative regulation of GTPase activity
IDA
PMID:22423108
ArfGAP1 is a GTPase activating protein for LRRK2: reciprocal...
UNDECIDED
Summary: Negative regulation of GTPase activity - needs verification.
Reason: Negative regulation of GTPase activity - needs verification.
Supporting Evidence:
PMID:22423108
ArfGAP1 is a GTPase activating protein for LRRK2: reciprocal regulation of ArfGAP1 by LRRK2.
GO:0010508 positive regulation of autophagy
IMP
PMID:22012985
Leucine-rich repeat kinase 2 regulates autophagy through a c...
MARK AS OVER ANNOTATED
Summary: Positive regulation of autophagy is a downstream effect of trafficking disruption, not core function.
Reason: Positive regulation of autophagy is a downstream effect of trafficking disruption, not core function.
Supporting Evidence:
PMID:22012985
Leucine-rich repeat kinase 2 regulates autophagy through a calcium-dependent pathway involving NAADP.
GO:0044325 transmembrane transporter binding
IPI
PMID:22012985
Leucine-rich repeat kinase 2 regulates autophagy through a c...
KEEP AS NON CORE
Summary: Transmembrane transporter binding - various interactions.
Reason: Transmembrane transporter binding - various interactions.
Supporting Evidence:
PMID:22012985
Leucine-rich repeat kinase 2 regulates autophagy through a calcium-dependent pathway involving NAADP.
GO:0003924 GTPase activity
IDA
PMID:21048939
ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange facto...
ACCEPT
Summary: GTPase activity is a core function of LRRK2 ROC domain. The ROC domain binds and hydrolyzes GTP.
Reason: GTPase activity is a core function of LRRK2 ROC domain. The ROC domain binds and hydrolyzes GTP.
Supporting Evidence:
PMID:21048939
ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange factor for leucine-rich repeat kinase 2.
GO:0004674 protein serine/threonine kinase activity
IDA
PMID:21048939
ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange facto...
ACCEPT
Summary: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Reason: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Supporting Evidence:
PMID:21048939
ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange factor for leucine-rich repeat kinase 2.
GO:0031267 small GTPase binding
IPI
PMID:21048939
ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange facto...
ACCEPT
Summary: Small GTPase binding - LRRK2 binds Rab substrates and other small GTPases.
Reason: Small GTPase binding - LRRK2 binds Rab substrates and other small GTPases.
Supporting Evidence:
PMID:21048939
ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange factor for leucine-rich repeat kinase 2.
GO:0043005 neuron projection
IDA
PMID:21048939
ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange facto...
KEEP AS NON CORE
Summary: Neuron projection is tissue-specific in neurons.
Reason: Neuron projection is tissue-specific in neurons.
Supporting Evidence:
PMID:21048939
ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange factor for leucine-rich repeat kinase 2.
GO:0043025 neuronal cell body
IDA
PMID:21048939
ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange facto...
KEEP AS NON CORE
Summary: Neuronal cell body is tissue-specific localization.
Reason: Neuronal cell body is tissue-specific localization.
Supporting Evidence:
PMID:21048939
ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange factor for leucine-rich repeat kinase 2.
GO:0004672 protein kinase activity
IDA
PMID:21850687
Mutations in LRRK2 increase phosphorylation of peroxiredoxin...
ACCEPT
Summary: Protein kinase activity is a core function of LRRK2, phosphorylating Rab GTPases and other substrates.
Reason: Protein kinase activity is a core function of LRRK2, phosphorylating Rab GTPases and other substrates.
Supporting Evidence:
PMID:21850687
Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death.
GO:0005739 mitochondrion
IDA
PMID:21850687
Mutations in LRRK2 increase phosphorylation of peroxiredoxin...
KEEP AS NON CORE
Summary: Mitochondrion - reported but not primary localization.
Reason: Mitochondrion - reported but not primary localization.
Supporting Evidence:
PMID:21850687
Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death.
GO:0035640 exploration behavior
IMP
PMID:20659558
Impaired dopaminergic neurotransmission and microtubule-asso...
MARK AS OVER ANNOTATED
Summary: Exploration behavior is a high-level organismal phenotype.
Reason: Exploration behavior is a high-level organismal phenotype.
Supporting Evidence:
PMID:20659558
2010 Jul 24. Impaired dopaminergic neurotransmission and microtubule-associated protein tau alterations in human LRRK2 transgenic mice.
GO:0060161 positive regulation of dopamine receptor signaling pathway
IMP
PMID:20659558
Impaired dopaminergic neurotransmission and microtubule-asso...
KEEP AS NON CORE
Summary: Positive regulation of dopamine receptor signaling pathway - tissue-specific.
Reason: Positive regulation of dopamine receptor signaling pathway - tissue-specific.
Supporting Evidence:
PMID:20659558
2010 Jul 24. Impaired dopaminergic neurotransmission and microtubule-associated protein tau alterations in human LRRK2 transgenic mice.
GO:0010508 positive regulation of autophagy
ISS
GO_REF:0000024
MARK AS OVER ANNOTATED
Summary: Positive regulation of autophagy is a downstream effect of trafficking disruption, not core function.
Reason: Positive regulation of autophagy is a downstream effect of trafficking disruption, not core function.
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
ISS
GO_REF:0000024
KEEP AS NON CORE
Summary: Positive regulation of proteasomal ubiquitin-dependent protein catabolic process - downstream.
Reason: Positive regulation of proteasomal ubiquitin-dependent protein catabolic process - downstream.
GO:0035564 regulation of kidney size
ISS
GO_REF:0000024
MARK AS OVER ANNOTATED
Summary: Regulation of kidney size is tissue-specific.
Reason: Regulation of kidney size is tissue-specific.
GO:0005515 protein binding
IPI
PMID:21159966
LRRK2 kinase regulates synaptic morphology through distinct ...
KEEP AS NON CORE
Summary: Protein binding is uninformative - LRRK2 has many documented interactors.
Reason: Protein binding is uninformative - LRRK2 has many documented interactors.
Supporting Evidence:
PMID:21159966
LRRK2 kinase regulates synaptic morphology through distinct substrates at the presynaptic and postsynaptic compartments of the Drosophila neuromuscular junction.
GO:0015631 tubulin binding
IDA
PMID:21159966
LRRK2 kinase regulates synaptic morphology through distinct ...
KEEP AS NON CORE
Summary: Tubulin binding - documented but not core function.
Reason: Tubulin binding - documented but not core function.
Supporting Evidence:
PMID:21159966
LRRK2 kinase regulates synaptic morphology through distinct substrates at the presynaptic and postsynaptic compartments of the Drosophila neuromuscular junction.
GO:0042391 regulation of membrane potential
IMP
PMID:21159966
LRRK2 kinase regulates synaptic morphology through distinct ...
KEEP AS NON CORE
Summary: Regulation of membrane potential - neuronal effect.
Reason: Regulation of membrane potential - neuronal effect.
Supporting Evidence:
PMID:21159966
LRRK2 kinase regulates synaptic morphology through distinct substrates at the presynaptic and postsynaptic compartments of the Drosophila neuromuscular junction.
GO:0048312 intracellular distribution of mitochondria
IMP
PMID:21159966
LRRK2 kinase regulates synaptic morphology through distinct ...
MARK AS OVER ANNOTATED
Summary: Intracellular distribution of mitochondria is secondary.
Reason: Intracellular distribution of mitochondria is secondary.
Supporting Evidence:
PMID:21159966
LRRK2 kinase regulates synaptic morphology through distinct substrates at the presynaptic and postsynaptic compartments of the Drosophila neuromuscular junction.
GO:0007528 neuromuscular junction development
IMP
PMID:21159966
LRRK2 kinase regulates synaptic morphology through distinct ...
MARK AS OVER ANNOTATED
Summary: Neuromuscular junction development is tissue-specific.
Reason: Neuromuscular junction development is tissue-specific.
Supporting Evidence:
PMID:21159966
LRRK2 kinase regulates synaptic morphology through distinct substrates at the presynaptic and postsynaptic compartments of the Drosophila neuromuscular junction.
GO:0005525 GTP binding
IDA
PMID:17260967
GTP binding is essential to the protein kinase activity of L...
ACCEPT
Summary: GTP binding is essential for LRRK2 ROC domain function and regulates kinase activity.
Reason: GTP binding is essential for LRRK2 ROC domain function and regulates kinase activity.
Supporting Evidence:
PMID:17260967
GTP binding is essential to the protein kinase activity of LRRK2, a causative gene product for familial Parkinson's disease.
GO:0034211 GTP-dependent protein kinase activity
IDA
PMID:17260967
GTP binding is essential to the protein kinase activity of L...
ACCEPT
Summary: GTP-dependent protein kinase activity reflects the intramolecular regulation between ROC GTPase and kinase domains.
Reason: GTP-dependent protein kinase activity reflects the intramolecular regulation between ROC GTPase and kinase domains.
Supporting Evidence:
PMID:17260967
GTP binding is essential to the protein kinase activity of LRRK2, a causative gene product for familial Parkinson's disease.
GO:0006979 response to oxidative stress
IMP
PMID:19692353
Leucine-Rich Repeat Kinase 2 interacts with Parkin, DJ-1 and...
MARK AS OVER ANNOTATED
Summary: Response to oxidative stress is pleiotropic/disease-related.
Reason: Response to oxidative stress is pleiotropic/disease-related.
Supporting Evidence:
PMID:19692353
Aug 19. Leucine-Rich Repeat Kinase 2 interacts with Parkin, DJ-1 and PINK-1 in a Drosophila melanogaster model of Parkinson's disease.
GO:0008340 determination of adult lifespan
IMP
PMID:19692353
Leucine-Rich Repeat Kinase 2 interacts with Parkin, DJ-1 and...
MARK AS OVER ANNOTATED
Summary: Determination of adult lifespan is pleiotropic.
Reason: Determination of adult lifespan is pleiotropic.
Supporting Evidence:
PMID:19692353
Aug 19. Leucine-Rich Repeat Kinase 2 interacts with Parkin, DJ-1 and PINK-1 in a Drosophila melanogaster model of Parkinson's disease.
GO:0040012 regulation of locomotion
IMP
PMID:19692353
Leucine-Rich Repeat Kinase 2 interacts with Parkin, DJ-1 and...
MARK AS OVER ANNOTATED
Summary: Regulation of locomotion is high-level organismal phenotype.
Reason: Regulation of locomotion is high-level organismal phenotype.
Supporting Evidence:
PMID:19692353
Aug 19. Leucine-Rich Repeat Kinase 2 interacts with Parkin, DJ-1 and PINK-1 in a Drosophila melanogaster model of Parkinson's disease.
GO:0004674 protein serine/threonine kinase activity
IDA
PMID:19576176
Lrrk2 phosphorylates alpha synuclein at serine 129: Parkinso...
ACCEPT
Summary: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Reason: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Supporting Evidence:
PMID:19576176
Lrrk2 phosphorylates alpha synuclein at serine 129: Parkinson disease implications.
GO:0034211 GTP-dependent protein kinase activity
IMP
PMID:17200152
Parkinson's disease-associated mutations in LRRK2 link enhan...
ACCEPT
Summary: GTP-dependent protein kinase activity reflects the intramolecular regulation between ROC GTPase and kinase domains.
Reason: GTP-dependent protein kinase activity reflects the intramolecular regulation between ROC GTPase and kinase domains.
Supporting Evidence:
PMID:17200152
Jan 2. Parkinson's disease-associated mutations in LRRK2 link enhanced GTP-binding and kinase activities to neuronal toxicity.
GO:0000165 MAPK cascade
IDA
PMID:17200152
Parkinson's disease-associated mutations in LRRK2 link enhan...
UNDECIDED
Summary: MAPK cascade - reported but may be downstream effect.
Reason: MAPK cascade - reported but may be downstream effect.
Supporting Evidence:
PMID:17200152
Jan 2. Parkinson's disease-associated mutations in LRRK2 link enhanced GTP-binding and kinase activities to neuronal toxicity.
GO:0004672 protein kinase activity
IDA
PMID:16269541
Parkinson's disease-associated mutations in leucine-rich rep...
ACCEPT
Summary: Protein kinase activity is a core function of LRRK2, phosphorylating Rab GTPases and other substrates.
Reason: Protein kinase activity is a core function of LRRK2, phosphorylating Rab GTPases and other substrates.
Supporting Evidence:
PMID:16269541
Parkinson's disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity.
GO:0004672 protein kinase activity
IDA
PMID:17442267
The R1441C mutation of LRRK2 disrupts GTP hydrolysis.
ACCEPT
Summary: Protein kinase activity is a core function of LRRK2, phosphorylating Rab GTPases and other substrates.
Reason: Protein kinase activity is a core function of LRRK2, phosphorylating Rab GTPases and other substrates.
Supporting Evidence:
PMID:17442267
The R1441C mutation of LRRK2 disrupts GTP hydrolysis.
GO:0004674 protein serine/threonine kinase activity
IDA
PMID:17200152
Parkinson's disease-associated mutations in LRRK2 link enhan...
ACCEPT
Summary: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Reason: Protein serine/threonine kinase activity is a core enzymatic function of LRRK2. The kinase domain phosphorylates Rab GTPases at their switch II regions.
Supporting Evidence:
PMID:17200152
Jan 2. Parkinson's disease-associated mutations in LRRK2 link enhanced GTP-binding and kinase activities to neuronal toxicity.
GO:0005096 GTPase activator activity
IDA
PMID:17442267
The R1441C mutation of LRRK2 disrupts GTP hydrolysis.
UNDECIDED
Summary: GTPase activator activity - LRRK2 has intrinsic GTPase activity but GAP activity on other GTPases is unclear.
Reason: GTPase activator activity - LRRK2 has intrinsic GTPase activity but GAP activity on other GTPases is unclear.
Supporting Evidence:
PMID:17442267
The R1441C mutation of LRRK2 disrupts GTP hydrolysis.
GO:0005525 GTP binding
IDA
PMID:16980962
Kinase activity of mutant LRRK2 mediates neuronal toxicity.
ACCEPT
Summary: GTP binding is essential for LRRK2 ROC domain function and regulates kinase activity.
Reason: GTP binding is essential for LRRK2 ROC domain function and regulates kinase activity.
Supporting Evidence:
PMID:16980962
Kinase activity of mutant LRRK2 mediates neuronal toxicity.
GO:0005525 GTP binding
IDA
PMID:17442267
The R1441C mutation of LRRK2 disrupts GTP hydrolysis.
ACCEPT
Summary: GTP binding is essential for LRRK2 ROC domain function and regulates kinase activity.
Reason: GTP binding is essential for LRRK2 ROC domain function and regulates kinase activity.
Supporting Evidence:
PMID:17442267
The R1441C mutation of LRRK2 disrupts GTP hydrolysis.
GO:0032473 cytoplasmic side of mitochondrial outer membrane
IDA
PMID:16269541
Parkinson's disease-associated mutations in leucine-rich rep...
KEEP AS NON CORE
Summary: Cytoplasmic side of mitochondrial outer membrane - reported.
Reason: Cytoplasmic side of mitochondrial outer membrane - reported.
Supporting Evidence:
PMID:16269541
Parkinson's disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity.
GO:0043068 positive regulation of programmed cell death
IDA
PMID:17200152
Parkinson's disease-associated mutations in LRRK2 link enhan...
MARK AS OVER ANNOTATED
Summary: Positive regulation of programmed cell death is disease-related.
Reason: Positive regulation of programmed cell death is disease-related.
Supporting Evidence:
PMID:17200152
Jan 2. Parkinson's disease-associated mutations in LRRK2 link enhanced GTP-binding and kinase activities to neuronal toxicity.
GO:0046777 protein autophosphorylation
IDA
PMID:16269541
Parkinson's disease-associated mutations in leucine-rich rep...
ACCEPT
Summary: Protein autophosphorylation is well-documented for LRRK2, including Ser1292 as an activity marker.
Reason: Protein autophosphorylation is well-documented for LRRK2, including Ser1292 as an activity marker.
Supporting Evidence:
PMID:16269541
Parkinson's disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity.
GO:0046777 protein autophosphorylation
IMP
PMID:16980962
Kinase activity of mutant LRRK2 mediates neuronal toxicity.
ACCEPT
Summary: Protein autophosphorylation is well-documented for LRRK2, including Ser1292 as an activity marker.
Reason: Protein autophosphorylation is well-documented for LRRK2, including Ser1292 as an activity marker.
Supporting Evidence:
PMID:16980962
Kinase activity of mutant LRRK2 mediates neuronal toxicity.
GO:0046777 protein autophosphorylation
IDA
PMID:17200152
Parkinson's disease-associated mutations in LRRK2 link enhan...
ACCEPT
Summary: Protein autophosphorylation is well-documented for LRRK2, including Ser1292 as an activity marker.
Reason: Protein autophosphorylation is well-documented for LRRK2, including Ser1292 as an activity marker.
Supporting Evidence:
PMID:17200152
Jan 2. Parkinson's disease-associated mutations in LRRK2 link enhanced GTP-binding and kinase activities to neuronal toxicity.
GO:0046777 protein autophosphorylation
IDA
PMID:17442267
The R1441C mutation of LRRK2 disrupts GTP hydrolysis.
ACCEPT
Summary: Protein autophosphorylation is well-documented for LRRK2, including Ser1292 as an activity marker.
Reason: Protein autophosphorylation is well-documented for LRRK2, including Ser1292 as an activity marker.
Supporting Evidence:
PMID:17442267
The R1441C mutation of LRRK2 disrupts GTP hydrolysis.
GO:0004672 protein kinase activity
IDA
PMID:16321986
The Parkinson disease causing LRRK2 mutation I2020T is assoc...
ACCEPT
Summary: Protein kinase activity is a core function of LRRK2, phosphorylating Rab GTPases and other substrates.
Reason: Protein kinase activity is a core function of LRRK2, phosphorylating Rab GTPases and other substrates.
Supporting Evidence:
PMID:16321986
Dec 1. The Parkinson disease causing LRRK2 mutation I2020T is associated with increased kinase activity.
GO:0005737 cytoplasm
IDA
PMID:16321986
The Parkinson disease causing LRRK2 mutation I2020T is assoc...
ACCEPT
Summary: Cytoplasm is a major localization for LRRK2.
Reason: Cytoplasm is a major localization for LRRK2.
Supporting Evidence:
PMID:16321986
Dec 1. The Parkinson disease causing LRRK2 mutation I2020T is associated with increased kinase activity.
GO:0005737 cytoplasm
IDA
PMID:16352719
Leucine-rich repeat kinase 2 (LRRK2) interacts with parkin, ...
ACCEPT
Summary: Cytoplasm is a major localization for LRRK2.
Reason: Cytoplasm is a major localization for LRRK2.
Supporting Evidence:
PMID:16352719
Leucine-rich repeat kinase 2 (LRRK2) interacts with parkin, and mutant LRRK2 induces neuronal degeneration.
GO:0031398 positive regulation of protein ubiquitination
IDA
PMID:16352719
Leucine-rich repeat kinase 2 (LRRK2) interacts with parkin, ...
KEEP AS NON CORE
Summary: Positive regulation of protein ubiquitination - related to parkin interaction.
Reason: Positive regulation of protein ubiquitination - related to parkin interaction.
Supporting Evidence:
PMID:16352719
Leucine-rich repeat kinase 2 (LRRK2) interacts with parkin, and mutant LRRK2 induces neuronal degeneration.
GO:0042803 protein homodimerization activity
IPI
PMID:16321986
The Parkinson disease causing LRRK2 mutation I2020T is assoc...
ACCEPT
Summary: Protein homodimerization activity - LRRK2 dimerization is well-documented.
Reason: Protein homodimerization activity - LRRK2 dimerization is well-documented.
Supporting Evidence:
PMID:16321986
Dec 1. The Parkinson disease causing LRRK2 mutation I2020T is associated with increased kinase activity.
GO:0046777 protein autophosphorylation
IDA
PMID:16321986
The Parkinson disease causing LRRK2 mutation I2020T is assoc...
ACCEPT
Summary: Protein autophosphorylation is well-documented for LRRK2, including Ser1292 as an activity marker.
Reason: Protein autophosphorylation is well-documented for LRRK2, including Ser1292 as an activity marker.
Supporting Evidence:
PMID:16321986
Dec 1. The Parkinson disease causing LRRK2 mutation I2020T is associated with increased kinase activity.

Core Functions

LRRK2 contains a kinase domain that phosphorylates Rab GTPases at threonine residues in their switch II regions. Primary substrates include Rab8A (Thr72), Rab10 (Thr73), Rab12 (Thr73), and Rab29 (Thr71).

The ROC (Ras of complex proteins) domain of LRRK2 binds and hydrolyzes GTP. GTP binding and hydrolysis regulate kinase domain activity through intramolecular signaling.

Molecular Function:
GTPase activity

LRRK2 ROC domain binds GTP, which is essential for regulating kinase activity. GTP binding status affects the conformation and enzymatic output of the protein.

Molecular Function:
GTP binding

References

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Deep Research

Falcon

(LRRK2-deep-research-falcon.md)

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πŸ“š Additional Documentation

Bioreason Rl Predictions

(LRRK2-bioreason-rl-predictions.md)

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Bioreason Rl Review

(LRRK2-bioreason-rl-review.md)

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πŸ“„ View Raw YAML

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