MAPK1

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0005634 nucleus	IBA GO_REF:0000033	ACCEPT	Summary: MAPK1/ERK2 localizes to the nucleus after stimulation. Reason: Nuclear localization is supported, but should be interpreted as stimulus-dependent rather than constitutive. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs. file:human/MAPK1/MAPK1-deep-research-falcon.md Do not infer “nuclear” localization from phospho-ERK alone. Nuclear accumulation can be partially uncoupled from TEY phosphorylation and depends on docking interactions and anchors.
GO:0005737 cytoplasm	IBA GO_REF:0000033	ACCEPT	Summary: MAPK1/ERK2 is cytoplasmic in quiescent cells. Reason: Cytoplasmic localization is a core supported localization for ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0035556 intracellular signal transduction	IBA GO_REF:0000033	ACCEPT	Summary: MAPK1 functions in intracellular signal transduction downstream of extracellular cues. Reason: Intracellular signal transduction is a supported core process for MAPK1 as the ERK2 kinase in the canonical MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md annotate ERK2 to MAPK cascade / ERK1 and ERK2 cascade and intracellular signal transduction; optionally include response to growth factor where the evidence is directly about upstream stimuli triggering ERK activation/translocation rather than downstream phenotypes.
GO:0004674 protein serine/threonine kinase activity	IBA GO_REF:0000033	ACCEPT	Summary: MAPK1/ERK2 is an ATP-dependent protein serine/threonine kinase. Reason: Serine/threonine protein kinase activity is a core molecular function of MAPK1; the more specific MAP kinase activity term should also be retained where present. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro.
GO:0007166 cell surface receptor signaling pathway	IBA GO_REF:0000033	MODIFY	Summary: MAPK1 responds to cell-surface receptor inputs, but its core role is the downstream intracellular ERK/MAPK cascade. Reason: Cell surface receptor signaling is too broad and upstream-oriented for the MAPK1 core function; ERK/MAPK cascade terms are more specific. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: GRANULARITY MISMATCH Sources checked: PANTHER:PTN008603019 · PTN008603019 SUPPORTS SOURCE BUT NOT TARGET The PAINT node's descendant evidence (including human MAPK1/MAPK3 themselves) genuinely supports receptor-initiated signaling, but the term is too broad and upstream-oriented for the core function; the ERK cascade replacement terms carry the same biology at the right granularity. Proposed replacements: ERK1 and ERK2 cascade MAPK cascade Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md annotate ERK2 to MAPK cascade / ERK1 and ERK2 cascade and intracellular signal transduction; optionally include response to growth factor where the evidence is directly about upstream stimuli triggering ERK activation/translocation rather than downstream phenotypes.
GO:0004672 protein kinase activity	IEA GO_REF:0000120	MODIFY	Summary: MAPK1 is a protein kinase, but this term is less specific than the supported serine/threonine MAP kinase terms. Reason: The generic protein kinase term should be replaced by protein serine/threonine kinase activity and MAP kinase activity, which capture MAPK1 specificity. Proposed replacements: protein serine/threonine kinase activity MAP kinase activity Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro.
GO:0004707 MAP kinase activity	IEA GO_REF:0000120	ACCEPT	Summary: MAPK1/ERK2 has MAP kinase activity. Reason: MAP kinase activity is the most specific core catalytic function for MAPK1/ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro. file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses.
GO:0005524 ATP binding	IEA GO_REF:0000002	ACCEPT	Summary: MAPK1 binds ATP as the phosphoryl donor for kinase catalysis. Reason: ATP binding is integral to the active-site chemistry of ERK2 kinase activity. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro.
GO:0005634 nucleus	IEA GO_REF:0000120	ACCEPT	Summary: MAPK1/ERK2 localizes to the nucleus after stimulation. Reason: Nuclear localization is supported, but should be interpreted as stimulus-dependent rather than constitutive. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs. file:human/MAPK1/MAPK1-deep-research-falcon.md Do not infer “nuclear” localization from phospho-ERK alone. Nuclear accumulation can be partially uncoupled from TEY phosphorylation and depends on docking interactions and anchors.
GO:0005737 cytoplasm	IEA GO_REF:0000120	ACCEPT	Summary: MAPK1/ERK2 is cytoplasmic in quiescent cells. Reason: Cytoplasmic localization is a core supported localization for ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005769 early endosome	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: This early endosome annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the early endosome location is a context-/pathway-specific or high-throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005770 late endosome	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: This late endosome annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the late endosome location is a context-/pathway-specific or high- throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005794 Golgi apparatus	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: This Golgi apparatus annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the Golgi apparatus location is a context-/pathway-specific or high-throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005813 centrosome	IEA GO_REF:0000044	MARK AS OVER ANNOTATED	Summary: This centrosome annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the centrosome location is a context-/pathway-specific or high- throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005819 spindle	IEA GO_REF:0000044	MARK AS OVER ANNOTATED	Summary: This spindle annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the spindle location is a context-/pathway-specific or high- throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005901 caveola	IEA GO_REF:0000044	MARK AS OVER ANNOTATED	Summary: This caveola annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the caveola location is a context-/pathway-specific or high- throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005925 focal adhesion	IEA GO_REF:0000120	MARK AS OVER ANNOTATED	Summary: This focal adhesion annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the focal adhesion location is a context-/pathway-specific or high-throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0006357 regulation of transcription by RNA polymerase II	IEA GO_REF:0000108	MARK AS OVER ANNOTATED	Summary: MAPK1 can affect transcriptional programs through downstream substrates, but broad regulation of RNA polymerase II transcription overstates the direct function. Reason: The direct MAPK1 function is kinase activity and ERK/MAPK cascade signaling; broad transcriptional regulation should not be inferred from pathway membership alone. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0007173 epidermal growth factor receptor signaling pathway	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: MAPK1 is activated in receptor and growth-factor signaling contexts, but these are stimulus-specific pathway contexts rather than the core conserved ERK2 function. Reason: Growth-factor and receptor-specific signaling annotations can be retained as non-core contexts; the core MAPK1 process is the intracellular ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md annotate ERK2 to MAPK cascade / ERK1 and ERK2 cascade and intracellular signal transduction; optionally include response to growth factor where the evidence is directly about upstream stimuli triggering ERK activation/translocation rather than downstream phenotypes.
GO:0010759 positive regulation of macrophage chemotaxis	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: This is a context-dependent downstream process linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0032206 positive regulation of telomere maintenance	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: Positive regulation of telomere maintenance is inferred from a high-throughput RNAi telomerase screen (PMID:21531765) plus electronic annotation. Reason: This is a high-throughput screen-derived, indirect pathway outcome rather than a direct MAPK1 function; it should not be elevated to a core ERK2 annotation without direct mechanistic evidence. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0032872 regulation of stress-activated MAPK cascade	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: This automated annotation places canonical ERK2 in a stress-activated MAPK cascade context. Reason: GO:0032872 is a stress-activated MAPK cascade term associated with JNK/p38 signaling, whereas MAPK1/ERK2 is curated here as the canonical ERK/MAPK cascade kinase. The automated inference overstates the evidence.
GO:0034198 cellular response to amino acid starvation	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: This is an amino-acid-starvation response annotation linked to ERK signaling context. Reason: The starvation-response annotation may be retained as non-core where the source evidence supports this context, but it should not be treated as the core conserved MAPK1 function. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0045202 synapse	IEA GO_REF:0000108	MARK AS OVER ANNOTATED	Summary: This synapse annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the synapse location is a context-/pathway-specific or high- throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0051403 stress-activated MAPK cascade	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: This automated annotation places canonical ERK2 in the stress-activated MAPK cascade. Reason: GO:0051403 refers to stress-activated MAPK cascade biology associated with JNK/p38 signaling, not the canonical ERK1/ERK2 cascade. The automated inference should not be retained as a valid non-core MAPK1 annotation.
GO:0051493 regulation of cytoskeleton organization	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: This is a context-dependent downstream process (regulation of cytoskeleton organization) linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function, which is ERK2 MAP kinase activity in the ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0061514 interleukin-34-mediated signaling pathway	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: This is a context-dependent downstream process linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0070371 ERK1 and ERK2 cascade	IEA GO_REF:0000117	ACCEPT	Summary: MAPK1/ERK2 is a central component of the ERK1 and ERK2 cascade. Reason: ERK1 and ERK2 cascade is a core pathway term for MAPK1 and is more specific than generic signaling process terms. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md annotate ERK2 to MAPK cascade / ERK1 and ERK2 cascade and intracellular signal transduction; optionally include response to growth factor where the evidence is directly about upstream stimuli triggering ERK activation/translocation rather than downstream phenotypes.
GO:0070849 response to epidermal growth factor	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: MAPK1 is activated in receptor and growth-factor signaling contexts, but these are stimulus-specific pathway contexts rather than the core conserved ERK2 function. Reason: Growth-factor and receptor-specific signaling annotations can be retained as non-core contexts; the core MAPK1 process is the intracellular ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md annotate ERK2 to MAPK cascade / ERK1 and ERK2 cascade and intracellular signal transduction; optionally include response to growth factor where the evidence is directly about upstream stimuli triggering ERK activation/translocation rather than downstream phenotypes.
GO:0072584 caveolin-mediated endocytosis	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: This is a context-dependent downstream process (caveolin-mediated endocytosis) linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function, which is ERK2 MAP kinase activity in the ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0090170 regulation of Golgi inheritance	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: This is a context-dependent downstream process (regulation of Golgi inheritance) linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function, which is ERK2 MAP kinase activity in the ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0097542 ciliary tip	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: This ciliary tip annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the ciliary tip location is a context-/pathway-specific or high- throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0106310 protein serine kinase activity	IEA GO_REF:0000116	MODIFY	Summary: MAPK1 can phosphorylate serine residues, but the serine-only term is incomplete for ERK2. Reason: ERK2 is a proline-directed serine/threonine MAP kinase; GO:0004674 and GO:0004707 better capture the supported activity than a serine-only activity term. Proposed replacements: protein serine/threonine kinase activity MAP kinase activity Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro.
GO:0120041 positive regulation of macrophage proliferation	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: This is a context-dependent downstream process linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:2000641 regulation of early endosome to late endosome transport	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: This is a context-dependent downstream process (regulation of early endosome to late endosome transport) linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function, which is ERK2 MAP kinase activity in the ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0005515 protein binding	IPI PMID:10415025 Direct suppression of TCR-mediated activation of extracellul...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:10415025; ERK2 has many reported interacting partners. ERK2 partner recognition is mediated structurally by the docking groove (D-recruitment site), which binds short linear D-motifs in substrates and regulators; this is directly illustrated by ERK2 crystal structures with bound D-motif peptides (PDB 2Y9Q, 3TEI, 4FMQ) that define docking-groove binding specificity (PMID:23047924). Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support. PMID:23047924 Mitogen-activated protein kinases (MAPKs) have a docking groove that interacts with linear "docking" motifs in binding partners. PMID:23047924 Crystal structures of four complexes of MAPKs with docking peptides ... revealed that the regions located between consensus positions in the docking motifs showed conformational diversity.
GO:0005515 protein binding	IPI PMID:10601328 A novel regulatory mechanism of MAP kinases activation and n...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:10601328; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:12592337 The protein tyrosine phosphatase HePTP regulates nuclear tra...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:12592337; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:12840032 Constitutive induction of p-Erk1/2 accompanied by reduced ac...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:12840032; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:15713638 Specific inactivation and nuclear anchoring of extracellular...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:15713638; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:16038800 Conditional expression of MAP kinase phosphatase-2 protects ...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:16038800; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:16286470 Cooperation of ERK and SCFSkp2 for MKP-1 destruction provide...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:16286470; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:16288922 New insights into the catalytic activation of the MAPK phosp...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:16288922; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:17255944 A MAPK docking site is critical for downregulation of Capicu...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:17255944; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:17255949 Mxi2 promotes stimulus-independent ERK nuclear translocation...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:17255949; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:18060821 Structural insights into the enzymatic mechanism of the path...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:18060821; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:18084305 Structural basis for the catalytic mechanism of phosphothreo...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:18084305; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:18463290 Human biliverdin reductase is an ERK activator; hBVR is an E...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:18463290; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:18616943 Phosphorylation of U24 from Human Herpes Virus type 6 (HHV-6...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:18616943; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:19494114 Tumor suppressor density-enhanced phosphatase-1 (DEP-1) inhi...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:19494114; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:21826244 TRAPPC4-ERK2 interaction activates ERK1/2, modulates its nuc...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:21826244; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:21908610 Phosphorylation of the kinase interaction motif in mitogen-a...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:21908610; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:21988832 Toward an understanding of the protein interaction network o...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:21988832; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:22521293 ETS-dependent p16INK4a and p21waf1/cip1 gene expression upon...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:22521293; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:23241949 MEK1 inactivates Myt1 to regulate Golgi membrane fragmentati...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:23241949; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:23455922 Interlaboratory reproducibility of large-scale human protein...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:23455922; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:23519423 Protein-peptide complex crystallization: a case study on the...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:23519423; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:23560844 Metal-dependent protein phosphatase 1A functions as an extra...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:23560844; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:23575685 Structure of ERK2 bound to PEA-15 reveals a mechanism for ra...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:23575685; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:23584453 Interaction with Shc prevents aberrant Erk activation in the...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:23584453; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:23602568 The protein interaction landscape of the human CMGC kinase g...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:23602568; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:25241761 Using an in situ proximity ligation assay to systematically ...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:25241761; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:25852190 Integrative analysis of kinase networks in TRAIL-induced apo...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:25852190; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:26267535 ERK2-Dependent Phosphorylation of CSN6 Is Critical in Colore...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:26267535; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:26733474 Identification of allosteric ERK2 inhibitors through in sili...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:26733474; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:27880917 Phenotypic and Interaction Profiling of the Human Phosphatas...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:27880917; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:29997244 LuTHy: a double-readout bioluminescence-based two-hybrid tec...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:29997244; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:31980649 Extensive rewiring of the EGFR network in colorectal cancer ...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:31980649; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:32296183 A reference map of the human binary protein interactome.	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:32296183; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:32707033 Kinase Interaction Network Expands Functional and Disease Ro...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:32707033; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:32814053 Interactome Mapping Provides a Network of Neurodegenerative ...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:32814053; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:33961781; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:34591642 A protein network map of head and neck cancer reveals PIK3CA...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:34591642; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:35271311; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:38884001 Mapping adipocyte interactome networks by HaloTag-enrichment...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:38884001; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:40205054 Multimodal cell maps as a foundation for structural and func...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:40205054; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:9632734 Activation of extracellular signal-regulated kinase 2 by a n...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:9632734; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:9788880 Isolation of the human genes encoding the pyst1 and Pyst2 ph...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:9788880; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0042802 identical protein binding	IPI PMID:16627622 Direct phosphorylation and regulation of poly(ADP-ribose) po...	KEEP AS NON CORE	Summary: MAPK1/ERK2 self-associates (homodimerizes); identical protein binding is supported by PMID:16627622. Reason: ERK2 dimerization is a documented property relevant to its cytoplasmic retention and scaffold interactions, but it is a regulatory/structural property rather than the core catalytic MAP kinase function.
GO:0042802 identical protein binding	IPI PMID:26267534 Small Molecule Inhibition of ERK Dimerization Prevents Tumor...	KEEP AS NON CORE	Summary: MAPK1/ERK2 self-associates (homodimerizes); identical protein binding is supported by PMID:26267534. Reason: ERK2 dimerization is a documented property relevant to its cytoplasmic retention and scaffold interactions, but it is a regulatory/structural property rather than the core catalytic MAP kinase function.
GO:0001784 phosphotyrosine residue binding	IEA GO_REF:0000107	MARK AS OVER ANNOTATED	Summary: This term assigns phosphotyrosine residue binding to ERK2. Reason: ERK2 substrate and partner recognition is mediated by D-domain/KIM and DEF/FXFP docking motifs rather than by a dedicated phosphotyrosine-binding module; this automated assignment is not supported as a core ERK2 molecular function. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0004674 protein serine/threonine kinase activity	IEA GO_REF:0000107	ACCEPT	Summary: MAPK1/ERK2 is an ATP-dependent protein serine/threonine kinase. Reason: Serine/threonine protein kinase activity is a core molecular function of MAPK1; the more specific MAP kinase activity term should also be retained where present. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro.
GO:0005739 mitochondrion	IEA GO_REF:0000107	MARK AS OVER ANNOTATED	Summary: This mitochondrion annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the mitochondrion location is a context-/pathway-specific or high- throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005829 cytosol	IEA GO_REF:0000107	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment. Reason: Cytosolic localization is consistent with ERK2 being predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0008286 insulin receptor signaling pathway	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: MAPK1 is activated in receptor and growth-factor signaling contexts, but these are stimulus-specific pathway contexts rather than the core conserved ERK2 function. Reason: Growth-factor and receptor-specific signaling annotations can be retained as non-core contexts; the core MAPK1 process is the intracellular ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md annotate ERK2 to MAPK cascade / ERK1 and ERK2 cascade and intracellular signal transduction; optionally include response to growth factor where the evidence is directly about upstream stimuli triggering ERK activation/translocation rather than downstream phenotypes.
GO:0008353 RNA polymerase II CTD heptapeptide repeat kinase activity	IEA GO_REF:0000107	MARK AS OVER ANNOTATED	Summary: This term assigns RNA polymerase II CTD heptapeptide repeat kinase activity to ERK2. Reason: ERK2 is a proline-directed Ser/Thr MAP kinase; while it phosphorylates Ser/Thr-Pro motifs, dedicated RNA Pol II CTD kinase activity is not a core supported ERK2 function and this ISS/IEA assignment overstates substrate specificity. The supported catalytic terms are MAP kinase activity and protein serine/threonine kinase activity. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro.
GO:0014044 Schwann cell development	IEA GO_REF:0000107	MARK AS OVER ANNOTATED	Summary: This is a distal or cell-type-specific outcome rather than a core MAPK1 function. Reason: The available synthesis supports ERK/MAPK cascade membership as core, but warns against promoting broad developmental or disease-associated outcomes to core MAPK1 functions without direct MAPK1-specific evidence. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0016301 kinase activity	IEA GO_REF:0000107	MODIFY	Summary: MAPK1 is a kinase, but this term is too broad for ERK2. Reason: The broad kinase activity annotation should use the more informative serine/threonine MAP kinase terms. Proposed replacements: protein serine/threonine kinase activity MAP kinase activity Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro.
GO:0019902 phosphatase binding	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: MAPK1 binds MAP kinase phosphatases such as DUSP6/MKP-3. Reason: Phosphatase binding is a supported regulatory interaction, but it is not the primary catalytic function of MAPK1. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md DUSP6/MKP-3 as cytoplasmic tether:** MKP-3/DUSP6 binds ERK2 and can dephosphorylate and tether inactive ERK2 in the cytoplasm; mutating MKP-3 NES or KIM abolishes cytoplasmic anchoring.
GO:0031143 pseudopodium	IEA GO_REF:0000107	MARK AS OVER ANNOTATED	Summary: This pseudopodium annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the pseudopodium location is a context-/pathway-specific or high- throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0035094 response to nicotine	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: This is a context-dependent downstream process (response to nicotine) linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function, which is ERK2 MAP kinase activity in the ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0038133 ERBB2-ERBB3 signaling pathway	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: MAPK1 is activated in receptor and growth-factor signaling contexts, but these are stimulus-specific pathway contexts rather than the core conserved ERK2 function. Reason: Growth-factor and receptor-specific signaling annotations can be retained as non-core contexts; the core MAPK1 process is the intracellular ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md annotate ERK2 to MAPK cascade / ERK1 and ERK2 cascade and intracellular signal transduction; optionally include response to growth factor where the evidence is directly about upstream stimuli triggering ERK activation/translocation rather than downstream phenotypes.
GO:0042552 myelination	IEA GO_REF:0000107	MARK AS OVER ANNOTATED	Summary: This is a distal or cell-type-specific outcome rather than a core MAPK1 function. Reason: The available synthesis supports ERK/MAPK cascade membership as core, but warns against promoting broad developmental or disease-associated outcomes to core MAPK1 functions without direct MAPK1-specific evidence. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0042802 identical protein binding	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: MAPK1/ERK2 self-associates (homodimerizes); identical protein binding is supported by GO_REF:0000107. Reason: ERK2 dimerization is a documented property relevant to its cytoplasmic retention and scaffold interactions, but it is a regulatory/structural property rather than the core catalytic MAP kinase function.
GO:0043415 positive regulation of skeletal muscle tissue regeneration	IEA GO_REF:0000107	MARK AS OVER ANNOTATED	Summary: This is a distal or cell-type-specific outcome rather than a core MAPK1 function. Reason: The available synthesis supports ERK/MAPK cascade membership as core, but warns against promoting broad developmental or disease-associated outcomes to core MAPK1 functions without direct MAPK1-specific evidence. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0048009 insulin-like growth factor receptor signaling pathway	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: MAPK1 is activated in receptor and growth-factor signaling contexts, but these are stimulus-specific pathway contexts rather than the core conserved ERK2 function. Reason: Growth-factor and receptor-specific signaling annotations can be retained as non-core contexts; the core MAPK1 process is the intracellular ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md annotate ERK2 to MAPK cascade / ERK1 and ERK2 cascade and intracellular signal transduction; optionally include response to growth factor where the evidence is directly about upstream stimuli triggering ERK activation/translocation rather than downstream phenotypes.
GO:0150078 positive regulation of neuroinflammatory response	IEA GO_REF:0000107	MARK AS OVER ANNOTATED	Summary: This is a distal or cell-type-specific outcome rather than a core MAPK1 function. Reason: The available synthesis supports ERK/MAPK cascade membership as core, but warns against promoting broad developmental or disease-associated outcomes to core MAPK1 functions without direct MAPK1-specific evidence. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0004707 MAP kinase activity	ISS GO_REF:0000024	ACCEPT	Summary: MAPK1/ERK2 has MAP kinase activity. Reason: MAP kinase activity is the most specific core catalytic function for MAPK1/ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro. file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses.
GO:0005829 cytosol	IDA PMID:22451653 Siglec-15 protein regulates formation of functional osteocla...	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment. Reason: Cytosolic localization is consistent with ERK2 being predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0043415 positive regulation of skeletal muscle tissue regeneration	ISS GO_REF:0000024	MARK AS OVER ANNOTATED	Summary: This is a distal or cell-type-specific outcome rather than a core MAPK1 function. Reason: The available synthesis supports ERK/MAPK cascade membership as core, but warns against promoting broad developmental or disease-associated outcomes to core MAPK1 functions without direct MAPK1-specific evidence. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0106310 protein serine kinase activity	ISS GO_REF:0000024	MODIFY	Summary: MAPK1 can phosphorylate serine residues, but the serine-only term is incomplete for ERK2. Reason: ERK2 is a proline-directed serine/threonine MAP kinase; GO:0004674 and GO:0004707 better capture the supported activity than a serine-only activity term. Proposed replacements: protein serine/threonine kinase activity MAP kinase activity Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro.
GO:0004674 protein serine/threonine kinase activity	IDA PMID:35831023 ERK2 MAP kinase regulates SUFU binding by multisite phosphor...	ACCEPT	Summary: MAPK1/ERK2 is an ATP-dependent protein serine/threonine kinase. Reason: Serine/threonine protein kinase activity is a core molecular function of MAPK1; the more specific MAP kinase activity term should also be retained where present. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro.
GO:0045880 positive regulation of smoothened signaling pathway	IDA PMID:35831023 ERK2 MAP kinase regulates SUFU binding by multisite phosphor...	KEEP AS NON CORE	Summary: ERK2 phosphorylation of GLI1 links MAPK1 to Smoothened/Hedgehog pathway output in this specific context. Reason: This is a supported pathway-specific downstream role from PMID:35831023, but the core MAPK1 function remains ERK2 serine/threonine MAP kinase activity in the ERK/MAPK cascade.
GO:0005654 nucleoplasm	IDA GO_REF:0000052	ACCEPT	Summary: MAPK1/ERK2 can accumulate in the nuclear compartment after stimulation. Reason: Nucleoplasmic localization is compatible with the supported stimulus-dependent nuclear accumulation of ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs. file:human/MAPK1/MAPK1-deep-research-falcon.md Do not infer “nuclear” localization from phospho-ERK alone. Nuclear accumulation can be partially uncoupled from TEY phosphorylation and depends on docking interactions and anchors.
GO:0005829 cytosol	IDA GO_REF:0000052	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment. Reason: Cytosolic localization is consistent with ERK2 being predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0004707 MAP kinase activity	TAS Reactome:R-HSA-111898	ACCEPT	Summary: MAPK1/ERK2 has MAP kinase activity. Reason: MAP kinase activity is the most specific core catalytic function for MAPK1/ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro. file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses.
GO:0004707 MAP kinase activity	TAS Reactome:R-HSA-445079	ACCEPT	Summary: MAPK1/ERK2 has MAP kinase activity. Reason: MAP kinase activity is the most specific core catalytic function for MAPK1/ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro. file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses.
GO:0004674 protein serine/threonine kinase activity	IDA PMID:38503280 A gut-derived hormone regulates cholesterol metabolism.	ACCEPT	Summary: MAPK1/ERK2 is an ATP-dependent protein serine/threonine kinase. Reason: Serine/threonine protein kinase activity is a core molecular function of MAPK1; the more specific MAP kinase activity term should also be retained where present. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro.
GO:0070371 ERK1 and ERK2 cascade	IDA PMID:15850461 The phosphorylation of CapZ-interacting protein (CapZIP) by ...	ACCEPT	Summary: MAPK1/ERK2 is a central component of the ERK1 and ERK2 cascade. Reason: ERK1 and ERK2 cascade is a core pathway term for MAPK1 and is more specific than generic signaling process terms. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md annotate ERK2 to MAPK cascade / ERK1 and ERK2 cascade and intracellular signal transduction; optionally include response to growth factor where the evidence is directly about upstream stimuli triggering ERK activation/translocation rather than downstream phenotypes.
GO:0150078 positive regulation of neuroinflammatory response	ISS GO_REF:0000024	MARK AS OVER ANNOTATED	Summary: This is a distal or cell-type-specific outcome rather than a core MAPK1 function. Reason: The available synthesis supports ERK/MAPK cascade membership as core, but warns against promoting broad developmental or disease-associated outcomes to core MAPK1 functions without direct MAPK1-specific evidence. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0004707 MAP kinase activity	IDA PMID:38503280 A gut-derived hormone regulates cholesterol metabolism.	ACCEPT	Summary: MAPK1/ERK2 has MAP kinase activity. Reason: MAP kinase activity is the most specific core catalytic function for MAPK1/ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro. file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses.
GO:0070098 chemokine-mediated signaling pathway	ISS GO_REF:0000024	KEEP AS NON CORE	Summary: This is a context-dependent downstream process (chemokine-mediated signaling pathway) linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function, which is ERK2 MAP kinase activity in the ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0000165 MAPK cascade	IDA PMID:15850461 The phosphorylation of CapZ-interacting protein (CapZIP) by ...	ACCEPT	Summary: MAPK1/ERK2 functions in the canonical MAPK cascade. Reason: MAPK cascade membership is a core biological process for ERK2, downstream of Ras-RAF-MEK signaling. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md annotate ERK2 to MAPK cascade / ERK1 and ERK2 cascade and intracellular signal transduction; optionally include response to growth factor where the evidence is directly about upstream stimuli triggering ERK activation/translocation rather than downstream phenotypes.
GO:0061514 interleukin-34-mediated signaling pathway	IGI PMID:26754294 miR-28-5p-IL-34-macrophage feedback loop modulates hepatocel...	KEEP AS NON CORE	Summary: This is a context-dependent downstream process linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0000165 MAPK cascade	IDA PMID:24854121 Endophilin-1 regulates blood-brain barrier permeability by c...	ACCEPT	Summary: MAPK1/ERK2 functions in the canonical MAPK cascade. Reason: MAPK cascade membership is a core biological process for ERK2, downstream of Ras-RAF-MEK signaling. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md annotate ERK2 to MAPK cascade / ERK1 and ERK2 cascade and intracellular signal transduction; optionally include response to growth factor where the evidence is directly about upstream stimuli triggering ERK activation/translocation rather than downstream phenotypes.
GO:0007173 epidermal growth factor receptor signaling pathway	IDA PMID:24854121 Endophilin-1 regulates blood-brain barrier permeability by c...	KEEP AS NON CORE	Summary: MAPK1 is activated in receptor and growth-factor signaling contexts, but these are stimulus-specific pathway contexts rather than the core conserved ERK2 function. Reason: Growth-factor and receptor-specific signaling annotations can be retained as non-core contexts; the core MAPK1 process is the intracellular ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md annotate ERK2 to MAPK cascade / ERK1 and ERK2 cascade and intracellular signal transduction; optionally include response to growth factor where the evidence is directly about upstream stimuli triggering ERK activation/translocation rather than downstream phenotypes.
GO:0005515 protein binding	IPI PMID:26942675 Mitochondria-Translocated PGK1 Functions as a Protein Kinase...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:26942675; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0032206 positive regulation of telomere maintenance	IMP PMID:21531765 High-throughput RNAi screening reveals novel regulators of t...	MARK AS OVER ANNOTATED	Summary: Positive regulation of telomere maintenance is inferred from a high-throughput RNAi telomerase screen (PMID:21531765) plus electronic annotation. Reason: This is a high-throughput screen-derived, indirect pathway outcome rather than a direct MAPK1 function; it should not be elevated to a core ERK2 annotation without direct mechanistic evidence. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0045542 positive regulation of cholesterol biosynthetic process	IDA PMID:38503280 A gut-derived hormone regulates cholesterol metabolism.	KEEP AS NON CORE	Summary: Positive regulation of cholesterol biosynthesis is an ERK2-linked downstream metabolic outcome reported in PMID:38503280. Reason: This is a context-dependent downstream metabolic process mediated via ERK signaling rather than a core conserved MAPK1 molecular function; retained as non-core. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0005515 protein binding	IPI PMID:11489891 MKP-7, a novel mitogen-activated protein kinase phosphatase,...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:11489891; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005515 protein binding	IPI PMID:32721402 Enhanced MAPK1 Function Causes a Neurodevelopmental Disorder...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:32721402; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005634 nucleus	IDA PMID:32721402 Enhanced MAPK1 Function Causes a Neurodevelopmental Disorder...	ACCEPT	Summary: MAPK1/ERK2 localizes to the nucleus after stimulation. Reason: Nuclear localization is supported, but should be interpreted as stimulus-dependent rather than constitutive. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs. file:human/MAPK1/MAPK1-deep-research-falcon.md Do not infer “nuclear” localization from phospho-ERK alone. Nuclear accumulation can be partially uncoupled from TEY phosphorylation and depends on docking interactions and anchors.
GO:0005737 cytoplasm	IDA PMID:32721402 Enhanced MAPK1 Function Causes a Neurodevelopmental Disorder...	ACCEPT	Summary: MAPK1/ERK2 is cytoplasmic in quiescent cells. Reason: Cytoplasmic localization is a core supported localization for ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005515 protein binding	IPI PMID:32051553 The EGFR-ZNF263 signaling axis silences SIX3 in glioblastoma...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:32051553; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0004674 protein serine/threonine kinase activity	IDA PMID:7588608 ERF: an ETS domain protein with strong transcriptional repre...	ACCEPT	Summary: MAPK1/ERK2 is an ATP-dependent protein serine/threonine kinase. Reason: Serine/threonine protein kinase activity is a core molecular function of MAPK1; the more specific MAP kinase activity term should also be retained where present. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro.
GO:0010759 positive regulation of macrophage chemotaxis	IGI PMID:26754294 miR-28-5p-IL-34-macrophage feedback loop modulates hepatocel...	KEEP AS NON CORE	Summary: This is a context-dependent downstream process linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0120041 positive regulation of macrophage proliferation	IGI PMID:26754294 miR-28-5p-IL-34-macrophage feedback loop modulates hepatocel...	KEEP AS NON CORE	Summary: This is a context-dependent downstream process linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0005515 protein binding	IPI PMID:15133037 The major vault protein is a novel substrate for the tyrosin...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:15133037; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005829 cytosol	TAS Reactome:R-HSA-9652816	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005788 endoplasmic reticulum lumen	TAS Reactome:R-HSA-9636296	MARK AS OVER ANNOTATED	Summary: This is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization; secretory or granule-lumen locations are likely pathway-context over-annotations for soluble ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs. file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005829 cytosol	TAS Reactome:R-HSA-9635739	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0004674 protein serine/threonine kinase activity	IDA PMID:26996940 Regulation of Microtubule Assembly by Tau and not by Pin1.	ACCEPT	Summary: MAPK1/ERK2 is an ATP-dependent protein serine/threonine kinase. Reason: Serine/threonine protein kinase activity is a core molecular function of MAPK1; the more specific MAP kinase activity term should also be retained where present. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro.
GO:0005886 plasma membrane	ISS GO_REF:0000024	MARK AS OVER ANNOTATED	Summary: This plasma membrane annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the plasma membrane location is a context-/pathway-specific or high-throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005901 caveola	ISS GO_REF:0000024	MARK AS OVER ANNOTATED	Summary: This caveola annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the caveola location is a context-/pathway-specific or high- throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0007611 learning or memory	NAS PMID:11404397 Beta-amyloid activates the mitogen-activated protein kinase ...	KEEP AS NON CORE	Summary: This is a context-dependent downstream process linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0035094 response to nicotine	ISS GO_REF:0000024	KEEP AS NON CORE	Summary: This is a context-dependent downstream process (response to nicotine) linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function, which is ERK2 MAP kinase activity in the ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0034198 cellular response to amino acid starvation	IDA PMID:11096076 Glutamine-dependent antiapoptotic interaction of human gluta...	KEEP AS NON CORE	Summary: This is an amino-acid-starvation response annotation linked to ERK signaling context. Reason: The starvation-response annotation may be retained as non-core where the source evidence supports this context, but it should not be treated as the core conserved MAPK1 function. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0051403 stress-activated MAPK cascade	IDA PMID:11096076 Glutamine-dependent antiapoptotic interaction of human gluta...	MARK AS OVER ANNOTATED	Summary: This stress-activated MAPK cascade annotation on MAPK1/ERK2 derives from a paper focused on ASK1/JNK stress signaling, not the canonical ERK cascade. Reason: GO:0051403 (stress-activated MAPK cascade) is associated with JNK/p38 (SAPK) signaling. PMID:11096076 concerns the Gln-dependent interaction of glutaminyl-tRNA synthetase with apoptosis signal-regulating kinase 1 (ASK1) and JNK/SAPK, not ERK2; assigning the stress-activated MAPK cascade to canonical ERK2 over-annotates MAPK1. Supporting Evidence: PMID:11096076 signal-regulating kinase 1 (ASK1) and c-Jun N-terminal kinase (JNK; also known as stress-activated protein kinase (SAPK)) in Gln-deprived cells
GO:0005576 extracellular region	TAS Reactome:R-HSA-6798751	MARK AS OVER ANNOTATED	Summary: This is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization; secretory or granule-lumen locations are likely pathway-context over-annotations for soluble ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs. file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005576 extracellular region	TAS Reactome:R-HSA-6800434	MARK AS OVER ANNOTATED	Summary: This is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization; secretory or granule-lumen locations are likely pathway-context over-annotations for soluble ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs. file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0035578 azurophil granule lumen	TAS Reactome:R-HSA-6798751	MARK AS OVER ANNOTATED	Summary: This is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization; secretory or granule-lumen locations are likely pathway-context over-annotations for soluble ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs. file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:1904813 ficolin-1-rich granule lumen	TAS Reactome:R-HSA-6800434	MARK AS OVER ANNOTATED	Summary: This is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization; secretory or granule-lumen locations are likely pathway-context over-annotations for soluble ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs. file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005515 protein binding	IPI PMID:24735981 MFAP3L activation promotes colorectal cancer cell invasion a...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:24735981; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0006468 protein phosphorylation	IDA PMID:23184662 Phosphorylation of eukaryotic elongation factor 2 (eEF2) by ...	ACCEPT	Summary: MAPK1 directly phosphorylates protein substrates. Reason: Protein phosphorylation is the direct biochemical output of the MAPK1 kinase activity. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro. file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 directly phosphorylates defined downstream targets such as Elk-1/ETS-family and other transcription-factor substrates in vitro and in cells; docking motifs/exosites help determine substrate recognition.
GO:0005634 nucleus	HDA PMID:16791210 Dynamic proteomics in individual human cells uncovers widesp...	ACCEPT	Summary: MAPK1/ERK2 localizes to the nucleus after stimulation. Reason: Nuclear localization is supported, but should be interpreted as stimulus-dependent rather than constitutive. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs. file:human/MAPK1/MAPK1-deep-research-falcon.md Do not infer “nuclear” localization from phospho-ERK alone. Nuclear accumulation can be partially uncoupled from TEY phosphorylation and depends on docking interactions and anchors.
GO:0005737 cytoplasm	HDA PMID:16791210 Dynamic proteomics in individual human cells uncovers widesp...	ACCEPT	Summary: MAPK1/ERK2 is cytoplasmic in quiescent cells. Reason: Cytoplasmic localization is a core supported localization for ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005737 cytoplasm	ISS GO_REF:0000024	ACCEPT	Summary: MAPK1/ERK2 is cytoplasmic in quiescent cells. Reason: Cytoplasmic localization is a core supported localization for ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0072686 mitotic spindle	ISS GO_REF:0000024	MARK AS OVER ANNOTATED	Summary: This mitotic spindle annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the mitotic spindle location is a context-/pathway-specific or high-throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005515 protein binding	IPI PMID:23847209 Pseudophosphatase STYX modulates cell-fate decisions and cel...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:23847209; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-198746	ACCEPT	Summary: MAPK1/ERK2 can accumulate in the nuclear compartment after stimulation; this is one of several Reactome reaction-level annotations placing ERK2 in the nucleoplasm. Reason: Nucleoplasmic localization is compatible with the supported stimulus-dependent nuclear accumulation of ERK2. The repeated Reactome nucleoplasm rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-198756	ACCEPT	Summary: MAPK1/ERK2 can accumulate in the nuclear compartment after stimulation; this is one of several Reactome reaction-level annotations placing ERK2 in the nucleoplasm. Reason: Nucleoplasmic localization is compatible with the supported stimulus-dependent nuclear accumulation of ERK2. The repeated Reactome nucleoplasm rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-199959	ACCEPT	Summary: MAPK1/ERK2 can accumulate in the nuclear compartment after stimulation; this is one of several Reactome reaction-level annotations placing ERK2 in the nucleoplasm. Reason: Nucleoplasmic localization is compatible with the supported stimulus-dependent nuclear accumulation of ERK2. The repeated Reactome nucleoplasm rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-203797	ACCEPT	Summary: MAPK1/ERK2 can accumulate in the nuclear compartment after stimulation; this is one of several Reactome reaction-level annotations placing ERK2 in the nucleoplasm. Reason: Nucleoplasmic localization is compatible with the supported stimulus-dependent nuclear accumulation of ERK2. The repeated Reactome nucleoplasm rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-3132737	ACCEPT	Summary: MAPK1/ERK2 can accumulate in the nuclear compartment after stimulation; this is one of several Reactome reaction-level annotations placing ERK2 in the nucleoplasm. Reason: Nucleoplasmic localization is compatible with the supported stimulus-dependent nuclear accumulation of ERK2. The repeated Reactome nucleoplasm rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-3857329	ACCEPT	Summary: MAPK1/ERK2 can accumulate in the nuclear compartment after stimulation; this is one of several Reactome reaction-level annotations placing ERK2 in the nucleoplasm. Reason: Nucleoplasmic localization is compatible with the supported stimulus-dependent nuclear accumulation of ERK2. The repeated Reactome nucleoplasm rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-450325	ACCEPT	Summary: MAPK1/ERK2 can accumulate in the nuclear compartment after stimulation; this is one of several Reactome reaction-level annotations placing ERK2 in the nucleoplasm. Reason: Nucleoplasmic localization is compatible with the supported stimulus-dependent nuclear accumulation of ERK2. The repeated Reactome nucleoplasm rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-5674387	ACCEPT	Summary: MAPK1/ERK2 can accumulate in the nuclear compartment after stimulation; this is one of several Reactome reaction-level annotations placing ERK2 in the nucleoplasm. Reason: Nucleoplasmic localization is compatible with the supported stimulus-dependent nuclear accumulation of ERK2. The repeated Reactome nucleoplasm rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-5675373	ACCEPT	Summary: MAPK1/ERK2 can accumulate in the nuclear compartment after stimulation; this is one of several Reactome reaction-level annotations placing ERK2 in the nucleoplasm. Reason: Nucleoplasmic localization is compatible with the supported stimulus-dependent nuclear accumulation of ERK2. The repeated Reactome nucleoplasm rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-9032751	ACCEPT	Summary: MAPK1/ERK2 can accumulate in the nuclear compartment after stimulation; this is one of several Reactome reaction-level annotations placing ERK2 in the nucleoplasm. Reason: Nucleoplasmic localization is compatible with the supported stimulus-dependent nuclear accumulation of ERK2. The repeated Reactome nucleoplasm rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-9610166	ACCEPT	Summary: MAPK1/ERK2 can accumulate in the nuclear compartment after stimulation; this is one of several Reactome reaction-level annotations placing ERK2 in the nucleoplasm. Reason: Nucleoplasmic localization is compatible with the supported stimulus-dependent nuclear accumulation of ERK2. The repeated Reactome nucleoplasm rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-9632910	ACCEPT	Summary: MAPK1/ERK2 can accumulate in the nuclear compartment after stimulation; this is one of several Reactome reaction-level annotations placing ERK2 in the nucleoplasm. Reason: Nucleoplasmic localization is compatible with the supported stimulus-dependent nuclear accumulation of ERK2. The repeated Reactome nucleoplasm rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-9725030	ACCEPT	Summary: MAPK1/ERK2 can accumulate in the nuclear compartment after stimulation; this is one of several Reactome reaction-level annotations placing ERK2 in the nucleoplasm. Reason: Nucleoplasmic localization is compatible with the supported stimulus-dependent nuclear accumulation of ERK2. The repeated Reactome nucleoplasm rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005654 nucleoplasm	TAS Reactome:R-HSA-9765960	ACCEPT	Summary: MAPK1/ERK2 can accumulate in the nuclear compartment after stimulation; this is one of several Reactome reaction-level annotations placing ERK2 in the nucleoplasm. Reason: Nucleoplasmic localization is compatible with the supported stimulus-dependent nuclear accumulation of ERK2. The repeated Reactome nucleoplasm rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005654 nucleoplasm	TAS Reactome:R-NUL-9009236	ACCEPT	Summary: MAPK1/ERK2 can accumulate in the nuclear compartment after stimulation; this is one of several Reactome reaction-level annotations placing ERK2 in the nucleoplasm. Reason: Nucleoplasmic localization is compatible with the supported stimulus-dependent nuclear accumulation of ERK2. The repeated Reactome nucleoplasm rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-109858	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-109862	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-109864	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-111898	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-1168459	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-2029469	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-3371531	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-418158	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-418163	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-418170	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-418176	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-418200	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-445079	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5654560	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5654562	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5654565	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5654566	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5672972	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5672973	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5672978	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5672980	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5674130	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5674132	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5674366	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5674373	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5674385	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5674387	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5674496	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5675194	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5675198	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5675206	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-5675376	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6802910	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6802911	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6802912	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6802914	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6802918	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6802919	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6802921	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6802922	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6802925	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6802926	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6802932	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6802933	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6802934	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6802935	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6802942	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6802943	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6803227	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6803230	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6803233	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6803234	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6811454	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-6811472	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9610152	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9610153	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9610154	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9610156	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9610166	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9626832	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9627089	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9632910	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9635743	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9636296	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9652165	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9656209	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9656211	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9656214	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9656215	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9657599	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9657603	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9657606	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9657608	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9731111	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9732753	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9734547	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9824582	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9824977	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9825759	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-HSA-9845033	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005829 cytosol	TAS Reactome:R-NUL-9619973	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment; this is one of many Reactome reaction-level annotations placing ERK2 in the cytosol. Reason: Cytosolic localization is a core supported localization for ERK2, which is predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. The numerous Reactome cytosol rows are group-accepted as consistent with this core localization. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0038127 ERBB signaling pathway	IDA PMID:15133037 The major vault protein is a novel substrate for the tyrosin...	KEEP AS NON CORE	Summary: MAPK1 is activated in receptor and growth-factor signaling contexts, but these are stimulus-specific pathway contexts rather than the core conserved ERK2 function. Reason: Growth-factor and receptor-specific signaling annotations can be retained as non-core contexts; the core MAPK1 process is the intracellular ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md annotate ERK2 to MAPK cascade / ERK1 and ERK2 cascade and intracellular signal transduction; optionally include response to growth factor where the evidence is directly about upstream stimuli triggering ERK activation/translocation rather than downstream phenotypes.
GO:0004674 protein serine/threonine kinase activity	IDA PMID:18794356 Extracellular signal-regulated kinase 2 (ERK2) phosphorylati...	ACCEPT	Summary: MAPK1/ERK2 is an ATP-dependent protein serine/threonine kinase. Reason: Serine/threonine protein kinase activity is a core molecular function of MAPK1; the more specific MAP kinase activity term should also be retained where present. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro.
GO:0005515 protein binding	IPI PMID:18794356 Extracellular signal-regulated kinase 2 (ERK2) phosphorylati...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:18794356; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0005634 nucleus	IDA PMID:18794356 Extracellular signal-regulated kinase 2 (ERK2) phosphorylati...	ACCEPT	Summary: MAPK1/ERK2 localizes to the nucleus after stimulation. Reason: Nuclear localization is supported, but should be interpreted as stimulus-dependent rather than constitutive. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs. file:human/MAPK1/MAPK1-deep-research-falcon.md Do not infer “nuclear” localization from phospho-ERK alone. Nuclear accumulation can be partially uncoupled from TEY phosphorylation and depends on docking interactions and anchors.
GO:0005737 cytoplasm	IDA PMID:18794356 Extracellular signal-regulated kinase 2 (ERK2) phosphorylati...	ACCEPT	Summary: MAPK1/ERK2 is cytoplasmic in quiescent cells. Reason: Cytoplasmic localization is a core supported localization for ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0070849 response to epidermal growth factor	IDA PMID:18794356 Extracellular signal-regulated kinase 2 (ERK2) phosphorylati...	KEEP AS NON CORE	Summary: MAPK1 is activated in receptor and growth-factor signaling contexts, but these are stimulus-specific pathway contexts rather than the core conserved ERK2 function. Reason: Growth-factor and receptor-specific signaling annotations can be retained as non-core contexts; the core MAPK1 process is the intracellular ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md annotate ERK2 to MAPK cascade / ERK1 and ERK2 cascade and intracellular signal transduction; optionally include response to growth factor where the evidence is directly about upstream stimuli triggering ERK activation/translocation rather than downstream phenotypes.
GO:0005515 protein binding	IPI PMID:22253824 Localization of nucleoporin Tpr to the nuclear pore complex ...	KEEP AS NON CORE	Summary: This is a generic protein-binding (GO:0005515) interaction annotation for MAPK1/ERK2 from PMID:22253824; ERK2 has many reported interacting partners. Reason: The generic protein binding term is uninformative for a multi-partner kinase like ERK2 and is retained only as a non-core interaction record. ERK partner recognition is motif/exosite-driven (KIM/D-domain, DEF/FXFP) and where a specific docking, substrate, scaffold, or phosphatase interaction is established a more informative molecular function term should be used instead of bare protein binding. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Because docking is motif-driven and structurally/biochemically dissectable, using only “protein binding” is typically uninformative and risks over-annotation. Additionally, yeast two-hybrid and peptide-based assays may capture indirect interactions or partial interfaces, so direct “binding” annotations should be limited to partners with orthogonal support.
GO:0004674 protein serine/threonine kinase activity	IDA PMID:15850461 The phosphorylation of CapZ-interacting protein (CapZIP) by ...	ACCEPT	Summary: MAPK1/ERK2 is an ATP-dependent protein serine/threonine kinase. Reason: Serine/threonine protein kinase activity is a core molecular function of MAPK1; the more specific MAP kinase activity term should also be retained where present. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro.
GO:0005634 nucleus	TAS PMID:19565474 The ERK signaling cascade--views from different subcellular ...	ACCEPT	Summary: MAPK1/ERK2 localizes to the nucleus after stimulation. Reason: Nuclear localization is supported, but should be interpreted as stimulus-dependent rather than constitutive. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs. file:human/MAPK1/MAPK1-deep-research-falcon.md Do not infer “nuclear” localization from phospho-ERK alone. Nuclear accumulation can be partially uncoupled from TEY phosphorylation and depends on docking interactions and anchors.
GO:0005739 mitochondrion	TAS PMID:19565474 The ERK signaling cascade--views from different subcellular ...	MARK AS OVER ANNOTATED	Summary: This mitochondrion annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the mitochondrion location is a context-/pathway-specific or high- throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005769 early endosome	TAS PMID:19565474 The ERK signaling cascade--views from different subcellular ...	MARK AS OVER ANNOTATED	Summary: This early endosome annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the early endosome location is a context-/pathway-specific or high-throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005770 late endosome	TAS PMID:19565474 The ERK signaling cascade--views from different subcellular ...	MARK AS OVER ANNOTATED	Summary: This late endosome annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the late endosome location is a context-/pathway-specific or high- throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005794 Golgi apparatus	TAS PMID:19565474 The ERK signaling cascade--views from different subcellular ...	MARK AS OVER ANNOTATED	Summary: This Golgi apparatus annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the Golgi apparatus location is a context-/pathway-specific or high-throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005829 cytosol	TAS PMID:19565474 The ERK signaling cascade--views from different subcellular ...	ACCEPT	Summary: MAPK1/ERK2 is present in the cytosolic/cytoplasmic compartment. Reason: Cytosolic localization is consistent with ERK2 being predominantly cytoplasmic in quiescent cells before stimulus-dependent nuclear accumulation. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK is predominantly cytoplasmic in quiescent cells and accumulates in the nucleus after activation by certain stimuli; nuclear ERK phosphorylates nuclear targets and contributes to induced gene expression programs.
GO:0005856 cytoskeleton	TAS PMID:19565474 The ERK signaling cascade--views from different subcellular ...	MARK AS OVER ANNOTATED	Summary: This cytoskeleton annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the cytoskeleton location is a context-/pathway-specific or high- throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005901 caveola	TAS PMID:19565474 The ERK signaling cascade--views from different subcellular ...	MARK AS OVER ANNOTATED	Summary: This caveola annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the caveola location is a context-/pathway-specific or high- throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0005925 focal adhesion	TAS PMID:19565474 The ERK signaling cascade--views from different subcellular ...	MARK AS OVER ANNOTATED	Summary: This focal adhesion annotation is not a supported core MAPK1 localization in this review. Reason: Falcon synthesis supports cytoplasmic/cytosolic and stimulus-dependent nuclear localization as core; the focal adhesion location is a context-/pathway-specific or high-throughput-derived assignment that should not be promoted to a core ERK2 localization without direct MAPK1-specific evidence in that compartment. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Prefer cytoplasm/nucleus over highly specific compartments unless direct localization evidence exists for MAPK1 itself in that context.
GO:0032872 regulation of stress-activated MAPK cascade	TAS PMID:19565474 The ERK signaling cascade--views from different subcellular ...	MARK AS OVER ANNOTATED	Summary: This TAS annotation assigns regulation of the stress-activated MAPK cascade to MAPK1/ERK2, citing a general ERK compartmentalization review. Reason: GO:0032872 (regulation of stress-activated MAPK cascade) is associated with JNK/p38 (SAPK) biology rather than the canonical ERK cascade. PMID:19565474 is a review of ERK signaling across subcellular compartments and does not establish ERK2 as a regulator of the stress-activated (JNK/p38) MAPK cascade; this is an over-annotation for canonical ERK2, consistent with the corresponding IEA row. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses.
GO:0051493 regulation of cytoskeleton organization	TAS PMID:19565474 The ERK signaling cascade--views from different subcellular ...	KEEP AS NON CORE	Summary: This is a context-dependent downstream process (regulation of cytoskeleton organization) linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function, which is ERK2 MAP kinase activity in the ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0072584 caveolin-mediated endocytosis	TAS PMID:19565474 The ERK signaling cascade--views from different subcellular ...	KEEP AS NON CORE	Summary: This is a context-dependent downstream process (caveolin-mediated endocytosis) linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function, which is ERK2 MAP kinase activity in the ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0090170 regulation of Golgi inheritance	TAS PMID:19565474 The ERK signaling cascade--views from different subcellular ...	KEEP AS NON CORE	Summary: This is a context-dependent downstream process (regulation of Golgi inheritance) linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function, which is ERK2 MAP kinase activity in the ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:2000641 regulation of early endosome to late endosome transport	TAS PMID:19565474 The ERK signaling cascade--views from different subcellular ...	KEEP AS NON CORE	Summary: This is a context-dependent downstream process (regulation of early endosome to late endosome transport) linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function, which is ERK2 MAP kinase activity in the ERK/MAPK cascade. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0010800 positive regulation of peptidyl-threonine phosphorylation	IDA PMID:16314496 Activation of TRAP/mediator subunit TRAP220/Med1 is regulate...	KEEP AS NON CORE	Summary: MAPK1 can increase phosphorylation of downstream threonine-containing protein substrates. Reason: The term is consistent with ERK2 substrate phosphorylation, but it is less central than the direct kinase activity and ERK/MAPK cascade annotations. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro. file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 directly phosphorylates defined downstream targets such as Elk-1/ETS-family and other transcription-factor substrates in vitro and in cells; docking motifs/exosites help determine substrate recognition.
GO:0070371 ERK1 and ERK2 cascade	IDA PMID:16314496 Activation of TRAP/mediator subunit TRAP220/Med1 is regulate...	ACCEPT	Summary: MAPK1/ERK2 is a central component of the ERK1 and ERK2 cascade. Reason: ERK1 and ERK2 cascade is a core pathway term for MAPK1 and is more specific than generic signaling process terms. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md annotate ERK2 to MAPK cascade / ERK1 and ERK2 cascade and intracellular signal transduction; optionally include response to growth factor where the evidence is directly about upstream stimuli triggering ERK activation/translocation rather than downstream phenotypes.
GO:0019902 phosphatase binding	IPI PMID:19494114 Tumor suppressor density-enhanced phosphatase-1 (DEP-1) inhi...	KEEP AS NON CORE	Summary: MAPK1 binds MAP kinase phosphatases such as DUSP6/MKP-3. Reason: Phosphatase binding is a supported regulatory interaction, but it is not the primary catalytic function of MAPK1. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md DUSP6/MKP-3 as cytoplasmic tether:** MKP-3/DUSP6 binds ERK2 and can dephosphorylate and tether inactive ERK2 in the cytoplasm; mutating MKP-3 NES or KIM abolishes cytoplasmic anchoring.
GO:0008353 RNA polymerase II CTD heptapeptide repeat kinase activity	ISS GO_REF:0000024	MARK AS OVER ANNOTATED	Summary: This term assigns RNA polymerase II CTD heptapeptide repeat kinase activity to ERK2. Reason: ERK2 is a proline-directed Ser/Thr MAP kinase; while it phosphorylates Ser/Thr-Pro motifs, dedicated RNA Pol II CTD kinase activity is not a core supported ERK2 function and this ISS/IEA assignment overstates substrate specificity. The supported catalytic terms are MAP kinase activity and protein serine/threonine kinase activity. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro.
GO:0004707 MAP kinase activity	TAS PMID:10706854 Eotaxin induces degranulation and chemotaxis of eosinophils ...	ACCEPT	Summary: MAPK1/ERK2 has MAP kinase activity. Reason: MAP kinase activity is the most specific core catalytic function for MAPK1/ERK2. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is a Ser/Thr MAPK that transfers the γ-phosphate of ATP to Ser/Thr-Pro motifs; like other protein kinases it binds ATP in an active-site cleft, with MgATP2− serving as the relevant phosphoryl donor in vitro. file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses.
GO:0006915 apoptotic process	TAS PMID:10958679 Hsp72-mediated suppression of c-Jun N-terminal kinase is imp...	KEEP AS NON CORE	Summary: This is a context-dependent downstream process linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0006935 chemotaxis	TAS PMID:10706854 Eotaxin induces degranulation and chemotaxis of eosinophils ...	KEEP AS NON CORE	Summary: This is a context-dependent downstream process linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.
GO:0007165 signal transduction	TAS PMID:1540184 Extracellular signal-regulated kinases in T cells: character...	MODIFY	Summary: MAPK1 participates in signal transduction, but the generic term should be replaced by more specific intracellular MAPK/ERK signaling terms. Reason: Generic signal transduction underspecifies MAPK1; intracellular signal transduction and ERK/MAPK cascade terms capture the supported biology more accurately. Proposed replacements: intracellular signal transduction ERK1 and ERK2 cascade Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md ERK2 is consistently positioned as a downstream effector in the Ras–Raf–MEK–ERK signal transduction cascade, converting extracellular cues (including growth factors/serum) into intracellular phosphorylation events and responses. file:human/MAPK1/MAPK1-deep-research-falcon.md annotate ERK2 to MAPK cascade / ERK1 and ERK2 cascade and intracellular signal transduction; optionally include response to growth factor where the evidence is directly about upstream stimuli triggering ERK activation/translocation rather than downstream phenotypes.
GO:0007268 chemical synaptic transmission	TAS PMID:10051431 Regulation of extracellular-signal regulated kinase and c-Ju...	KEEP AS NON CORE	Summary: This is a context-dependent downstream process linked to ERK signaling. Reason: The process may be retained as non-core where the source evidence supports a specific context, but it should not be treated as the core conserved MAPK1 function. Supporting Evidence: file:human/MAPK1/MAPK1-deep-research-falcon.md Many sources link ERK signaling to proliferation/differentiation and disease contexts, but these are typically emergent outcomes of the pathway rather than direct MAPK1 enzymatic functions.

Gene Description

Existing Annotations Review

Core Functions

MAPK1/ERK2 is an ATP-dependent, proline-directed serine/threonine MAP kinase. After MEK1/2-mediated activation, ERK2 phosphorylates protein substrates and transmits canonical Ras-RAF-MEK-ERK signals to cytosolic and nuclear targets.

ATP binding is a core active-site function of MAPK1 because ATP/MgATP provides the phosphoryl group used for ERK2 serine/threonine protein kinase catalysis.

References

Suggested Questions for Experts

Suggested Experiments

Deep Research

Falcon

📚 Additional Documentation

Notes

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