MIR155

RNAcentral ID: URS000062749E_9606
Organism: Homo sapiens
Review Status: COMPLETE
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Gene Description

MIR155 encodes miR-155, a microRNA whose core molecular role is to guide Argonaute/RISC to target RNAs for miRNA-mediated post-transcriptional gene regulation. MIR155 is important in immune responses and oncogenesis, but those are context-specific regulatory outcomes rather than separate molecular functions.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0035195 miRNA-mediated post-transcriptional gene silencing
IEA
GO_REF:0000115 		ACCEPT
Summary: RNAcentral/Rfam annotation for the primary biological process that MIR155 participates in.
Reason: MIR155 is a microRNA precursor and its mature product participates in miRNA-mediated post-transcriptional gene silencing.
Supporting Evidence:
PMID:23416982
MicroRNA-155 (miR-155) is an established oncomiR in breast cancer and regulates several pro-oncogenic pathways.
file:human/MIR155/MIR155-deep-research-falcon.md
Differential Ago HITS-CLIP/dCLIP comparing WT and miR-155-knockout activated CD4+ T cells identified miR-155-dependent Ago-binding sites, supporting miR-155-mediated post-transcriptional silencing.
GO:0016442 RISC complex
IEA
GO_REF:0000115 		ACCEPT
Summary: RNAcentral/Rfam annotation placing MIR155 in the RNA-induced silencing complex context.
Reason: Mature miRNAs guide RISC-mediated target repression, so this RNAcentral annotation is consistent with MIR155 biology.
Supporting Evidence:
file:human/MIR155/MIR155-deep-research-falcon.md
Direct Argonaute immunoprecipitation in human macrophages provides strong evidence that miR-155 strands are physically present in Argonaute/RISC complexes.
GO:0003730 mRNA 3'-UTR binding
NAS NEW
Summary: Added to align the core MIR155 molecular function with the annotation block. Mature miR-155 guides Argonaute/RISC to target RNAs, with target recognition mediated by base-pairing to sites that include mRNA 3'-UTRs.
Reason: Falcon found direct evidence for miR-155-dependent Argonaute occupancy at target sites, supporting the core mRNA 3'-UTR binding function used in the core_functions block.
Supporting Evidence:
file:human/MIR155/MIR155-deep-research-falcon.md
Differential Ago HITS-CLIP/dCLIP comparing WT and miR-155-knockout activated CD4+ T cells identified miR-155-dependent Ago-binding sites.

Core Functions

miRNA guide RNA activity in Argonaute/RISC-mediated post-transcriptional gene silencing

Molecular Function:
mRNA 3'-UTR binding
Directly Involved In:
miRNA-mediated post-transcriptional gene silencing
In Complex:
RISC complex
Supporting Evidence:
  • file:human/MIR155/MIR155-deep-research-falcon.md
    Strong direct evidence exists for miR-155 association with Argonaute/RISC and for miR-155-guided post-transcriptional regulation.

References

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Suggested Questions for Experts

Q: What are the complete set of target mRNAs regulated by MIR155 in different cell types?

Suggested experts: microRNA researchers, cancer biologists

Q: How does MIR155 expression change during immune cell activation and differentiation?

Suggested experts: immunologists, T cell biologists

Suggested Experiments

Experiment: CLIP-seq analysis to identify direct MIR155 target sites

Hypothesis: MIR155 directly binds to specific sequences in target mRNAs

Type: high-throughput sequencing

Experiment: Single-cell RNA-seq of immune cells with MIR155 knockdown

Hypothesis: MIR155 regulates immune cell differentiation programs

Type: single-cell analysis

Deep Research

Falcon

(MIR155-deep-research-falcon.md)

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