MTIF3 encodes mitochondrial translation initiation factor 3 (mtIF3, IF3mt), a nuclear-encoded member of the IF-3 family imported into the mitochondrial matrix via an N-terminal presequence. Its conserved N- and C-terminal domains are flanked by mitochondria-specific extensions. mtIF3 binds the small (28S) mitoribosomal subunit with high affinity and acts as an anti-association factor, dissociating 55S mitoribosomes into 28S and 39S subunits. It also prevents initiator fMet-tRNA from binding to the 28S subunit in the absence of mRNA. Most leaderless mitochondrial mRNAs can be initiated on intact 55S monosomes without mtIF3, and MTIF3-knockout human cells retain mitochondrial translation. The factor is required for translation of ATP6 from the bicistronic ATP8/ATP6 transcript and for coordinated, correctly regulated initiation in vivo. Heart- and skeletal-muscle-specific loss of Mtif3 in mice causes uncoordinated mitochondrial protein synthesis, loss of specific respiratory complexes and cardiomyopathy.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003743 translation initiation factor activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference of translation initiation factor activity for the IF-3 family. Reason: Purified IF3mt promotes initiation complex formation on mitochondrial 55S ribosomes and is required for correct initiation in vivo. Supporting Evidence: PMID:12095986 Purified IF3(mt) promotes initiation complex formation on mitochondrial 55 S ribosomes in the presence of mitochondrial initiation factor 2 (IF2(mt)), [(35)S]fMet-tRNA, and either poly(A,U,G) or an in vitro transcript of the cytochrome oxidase subunit II gene as mRNA. |
| GO:0003743 translation initiation factor activity | IEA GO_REF:0000002 | ACCEPT | Summary: InterPro IF-3 domain mapping. Reason: Correct; consistent with biochemical evidence. Supporting Evidence: PMID:12095986 Purified IF3(mt) promotes initiation complex formation on mitochondrial 55 S ribosomes |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: Binary yeast two-hybrid interaction from a proteome-scale human interactome map. Reason: Uninformative protein binding term from a high-throughput yeast two-hybrid screen, with no demonstrated relevance to the function of a matrix-resident initiation factor. Supporting Evidence: PMID:25416956 Here, we describe a systematic map of ?14,000 high-quality human binary protein-protein interactions. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary yeast two-hybrid interaction from the HuRI all-by-all human reference interactome (one of 76 such rows for MTIF3). Reason: Uninformative protein binding term. The HuRI partners of MTIF3 are mostly membrane, secreted or extracellular proteins (for example APOD, LDLR, CD47, CXCL9, FETUB) that never share a compartment with a matrix-resident mitochondrial initiation factor. These yeast two-hybrid hits do not inform MTIF3 function. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. |
| GO:0005739 mitochondrion | HTP PMID:34800366 Quantitative high-confidence human mitochondrial proteome an... | ACCEPT | Summary: Detected in the high-confidence human mitochondrial proteome. Reason: Correct. Supporting Evidence: PMID:12095986 MitoProt II predicts a 97% probability that this protein will be localized in mitochondria |
| GO:0005739 mitochondrion | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference of mitochondrial localisation for the eukaryotic mitochondrial IF-3 subfamily. Reason: Correct. Supporting Evidence: file:human/MTIF3/MTIF3-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion |
| GO:0005739 mitochondrion | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt subcellular location mapping. Reason: Correct. Supporting Evidence: file:human/MTIF3/MTIF3-uniprot.txt SUBCELLULAR LOCATION: Mitochondrion |
| GO:0005739 mitochondrion | ISS PMID:12095986 Identification of mammalian mitochondrial translational init... | ACCEPT | Summary: Sequence-similarity localisation assigned with the IF3mt identification paper. The with/from field names bovine IF-2mt (P46198) rather than an IF-3 family member. Reason: The localisation is correct for MTIF3 whatever the cited similarity partner; the paper predicts mitochondrial targeting from the sequence. Supporting Evidence: PMID:12095986 MitoProt II predicts a 97% probability that this protein will be localized in mitochondria |
| GO:0006413 translational initiation | IEA GO_REF:0000002 | MODIFY | Summary: InterPro mapping of translational initiation. Reason: Correct but generic; MTIF3 acts only in mitochondrial translation. Proposed replacements: mitochondrial translational initiation Supporting Evidence: PMID:31903419 Ribosome profiling shows that MTIF3 is required for recognition and regulation of translation initiation of mitochondrial mRNAs and for coordinated assembly of OXPHOS complexes in vivo. |
| GO:0008135 translation factor activity, RNA binding | IDA PMID:12095986 Identification of mammalian mitochondrial translational init... | MODIFY | Summary: Purified IF3mt stimulated initiation complex formation on mitochondrial ribosomes. Reason: The experiment demonstrates initiation activity specifically, so the child term translation initiation factor activity is more informative than the general translation factor term. Proposed replacements: translation initiation factor activity Supporting Evidence: PMID:12095986 Purified IF3(mt) promotes initiation complex formation on mitochondrial 55 S ribosomes |
| GO:0032790 ribosome disassembly | IDA PMID:12095986 Identification of mammalian mitochondrial translational init... | ACCEPT | Summary: IF3mt shifts the equilibrium between 55S mitoribosomes and their subunits toward dissociation. Reason: The GO definition of ribosome disassembly explicitly includes dissociation of ribosomal subunits. The anti-association and 55S-dissociating activity of IF3mt is shown by several independent biochemical studies, is abolished by C-terminal domain mutations that also block initiation complex formation, and is explained by the position of the C-terminal domain on the 28S subunit. Supporting Evidence: PMID:12095986 IF3(mt) shifts the equilibrium between the 55 S mitochondrial ribosome and its subunits toward subunit dissociation. PMID:18056078 IF3(mt) is very effective in dissociating 55S ribosomes. PMID:19239245 These mutations appear to lead to a factor defective in the ability to displace the large (39S) subunit of the ribosome from the 55S monosomes in an active process. |
| GO:0043024 ribosomal small subunit binding | IDA PMID:12095986 Identification of mammalian mitochondrial translational init... | MODIFY | Summary: IF3mt binds the 28S small subunit of the mitochondrial ribosome. Reason: Correct; the more precise term mitochondrial small ribosomal subunit binding states which subunit is bound. Binding is high-affinity (Kd about 30 nM) and explained structurally by unique contacts of the N-terminal domain. Proposed replacements: mitochondrial small ribosomal subunit binding Supporting Evidence: PMID:18056078 The K(d) for the binding of IF3(mt) to 28S subunits is approximately 30 nM. PMID:30677741 Unique contacts observed between the 28S subunit and N-terminal domain of IF3mt explain its unusually high affinity for the 28S subunit |
| GO:0070124 mitochondrial translational initiation | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference of mitochondrial translational initiation. Reason: Core process, supported by knockout and ribosome profiling in mice and by human knockout cells. Supporting Evidence: PMID:31903419 Ribosome profiling shows that MTIF3 is required for recognition and regulation of translation initiation of mitochondrial mRNAs and for coordinated assembly of OXPHOS complexes in vivo. PMID:36629253 mtIF3 activity is not required for translation of leaderless mitochondrial transcripts but is essential for translation of ATP6 in the case of the bicistronic ATP8/ATP6 transcript |
| GO:0005759 mitochondrial matrix | IC PMID:12095986 Identification of mammalian mitochondrial translational init... | NEW | Summary: Proposed matrix localisation, inferred from the activity of IF3mt on mitochondrial 28S subunits and 55S ribosomes, which reside in the matrix. Reason: GOA has only the parent term mitochondrion for MTIF3. Its site of action, the mitoribosome, is in the matrix, which is the precise compartment; MTIF2, with which it acts, carries mitochondrial matrix by IC. Supporting Evidence: PMID:12095986 IF3(mt) shifts the equilibrium between the 55 S mitochondrial ribosome and its subunits toward subunit dissociation. |
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Download this section (compressed HTML)Q: Why is mtIF3 dispensable for most leaderless mt-mRNAs yet essential for ATP6 initiation, and does it act at ATP6 by start-codon recognition or by recycling post-termination ribosomes for re-initiation?
Q: Mouse Mtif3 loss increases but uncoordinates mitochondrial protein synthesis, whereas human knockout cells show a selective ATP6 defect; do these differences reflect tissue context or species?
Experiment: Mitoribosome profiling in human MTIF3-knockout cells rescued with C-terminal-domain anti-association mutants (residues 170-175) or C-terminal-extension mutants (247-248), to separate the subunit-dissociation and initiator-tRNA-proofreading activities and map which of them is needed for ATP6 initiation.
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