ProtNLM2 External predictions
View prediction review YAML · NARF-protnlm-predictions-review.yaml · Review status: COMPLETE
The selected short NARF product has no demonstrated 4Fe–4S cluster binding. The accompanying function paragraph confuses NARF/IOP2 with the experimentally distinguished CIA paralog NARFL/IOP1.
Source documents: genes/human/NARF/NARF-protnlm-source.json · genes/human/NARF/NARF-notes.md
Review score: 2 = concordant with evidence; 1 = uncertain; 0 = discordant with evidence. This is an assessment score, not a model probability.
UNC — Uncertain Review score: 1/2
Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-10 · file:human/NARF/NARF-protnlm-source.json
Review rationale: Hydrogenase-family homology makes iron-sulfur coordination a hypothesis, but it is not demonstrated for this NARF product. J3KS48 lacks much of the 456-residue reference protein. The IOP1/IOP2 knockdown comparison distinguishes CIA function but does not itself test cluster occupancy of NARF; it therefore cannot refute or validate this binding claim.
Supporting Evidence:
- file:human/NARF/NARF-bioinformatics/RESULTS.md: "Selected J3KS48 has 217 residues; reference Q9UHQ1 has 456 residues."
- PMID:18270200: "Knockdown of IOP2, in contrast, had no effect on either."