id: J3KS48
gene_symbol: NARF
taxon:
  id: NCBITaxon:9606
  label: Homo sapiens
status: COMPLETE
description: The selected short NARF product has no demonstrated 4Fe–4S cluster binding. The accompanying
  function paragraph confuses NARF/IOP2 with the experimentally distinguished CIA paralog NARFL/IOP1.
source_documents:
- genes/human/NARF/NARF-protnlm-source.json
- genes/human/NARF/NARF-notes.md
references:
- id: GO_REF:0000044
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping,
    accompanied by conservative changes to GO terms applied by UniProt
  findings: []
- id: PMID:10514485
  title: Prenylated prelamin A interacts with Narf, a novel nuclear protein.
  findings:
  - statement: Human NARF constructs localize to nuclei and bind prenylated prelamin A; the paper does
      not establish activity of the selected 217-residue product.
    supporting_text: 'When a FLAG epitope-tagged Narf is expressed

      in HeLa cells, it is exclusively nuclear and partially co-localizes with the

      nuclear lamina.'
  full_text_unavailable: true
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: Primary report checked against the cached abstract/full text; the evidence scope is
      stated in the finding.
- id: PMID:18270200
  title: A role for IOP1 in mammalian cytosolic iron-sulfur protein biogenesis.
  findings:
  - statement: Compared with IOP1 depletion, IOP2/NARF depletion did not impair cytosolic or mitochondrial
      aconitase activity, distinguishing the paralogs in this tested CIA phenotype.
    supporting_text: 'Knockdown of IOP2, in contrast, had no

      effect on either.'
  full_text_unavailable: true
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: Primary report checked against the cached abstract/full text; the evidence scope is
      stated in the finding.
- id: file:human/NARF/NARF-bioinformatics/RESULTS.md
  title: Exact NARF product-to-reference sequence mapping
- id: file:human/NARF/NARF-protnlm-source.json
  title: Frozen exact-input ProtNLM output and UniProt sequence for J3KS48
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-10
  source_reference_id: file:human/NARF/NARF-protnlm-source.json
  predicted_term:
    id: GO:0051539
    label: 4 iron, 4 sulfur cluster binding
  predicted_term_type: GO_MF
  review:
    assessment: UNC
    confidence_score: 1
    summary: Hydrogenase-family homology makes iron-sulfur coordination a hypothesis, but it is not demonstrated
      for this NARF product. J3KS48 lacks much of the 456-residue reference protein. The IOP1/IOP2 knockdown
      comparison distinguishes CIA function but does not itself test cluster occupancy of NARF; it therefore
      cannot refute or validate this binding claim.
    supported_by:
    - reference_id: file:human/NARF/NARF-bioinformatics/RESULTS.md
      supporting_text: Selected J3KS48 has 217 residues; reference Q9UHQ1 has 456 residues.
    - reference_id: PMID:18270200
      supporting_text: 'Knockdown of IOP2, in contrast, had no

        effect on either.'