id: Q8TB37
gene_symbol: NUBPL
product_type: PROTEIN
status: INITIALIZED
taxon:
  id: NCBITaxon:9606
  label: Homo sapiens
description: >-
  NUBPL (nucleotide-binding protein-like; also called IND1 homolog / huInd1) is a
  mitochondrial iron-sulfur cluster transfer protein of the Mrp/NBP35 family of P-loop
  NTPase Fe-S carrier proteins. Synthesized with an N-terminal mitochondrial transit
  peptide and imported into the mitochondrial matrix, it binds a single [4Fe-4S] cluster
  in a labile fashion via a conserved CXXC motif and acts as a dedicated assembly factor
  that delivers Fe-S cofactor(s) to subunits of respiratory chain Complex I
  (NADH:ubiquinone oxidoreductase). NUBPL is not a structural subunit of the Complex I
  holoenzyme; rather it is required for maturation of the peripheral (matrix) arm, and its
  loss leads to failure to accumulate Fe-S-containing subunits such as NDUFS1 and NDUFV1,
  reduced Complex I activity, remodeling of respiratory supercomplexes, and altered
  mitochondrial morphology. The protein also carries a Walker-A/P-loop ATP-binding motif
  characteristic of the family. In humans, biallelic loss-of-function variants in NUBPL
  cause mitochondrial complex I deficiency, nuclear type 21 (MC1DN21), a
  leukoencephalopathy with a distinctive MRI pattern.
alternative_products:
- name: '1'
  id: Q8TB37-1
- name: '2'
  id: Q8TB37-2
  sequence_note: VSP_020985, VSP_008797
existing_annotations:
- term:
    id: GO:0005739
    label: mitochondrion
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: is_active_in
  review:
    summary: >-
      Phylogenetic (IBA) localization to mitochondrion. Correct but generic relative to
      the experimentally supported and more specific mitochondrial matrix localization.
    action: MARK_AS_OVER_ANNOTATED
    reason: >-
      NUBPL is experimentally localized to mitochondrion (IDA, PMID:19752196) and more
      specifically to the mitochondrial matrix (Reactome TAS, ARBA IEA), consistent with
      a matrix-soluble assembly factor. The broad IBA "mitochondrion" call is superseded
      by the specific matrix annotation and is not treated as a distinct core localization.
    propagation_review:
      root_cause: NO_FAILURE_NON_CORE
      failure_modes:
      - GRANULARITY_MISMATCH
    supported_by:
    - reference_id: file:human/NUBPL/NUBPL-uniprot.txt
      supporting_text: "SUBCELLULAR LOCATION: Mitochondrion"
- term:
    id: GO:0032981
    label: mitochondrial respiratory chain complex I assembly
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  review:
    summary: >-
      Phylogenetic (IBA) assignment of the core biological process. This matches the
      experimentally established role of NUBPL/IND1 as an assembly factor critically
      required for respiratory Complex I.
    action: ACCEPT
    reason: >-
      Independently supported by experimental IMP evidence (PMID:19752196), where huInd1
      knockdown strongly decreased Complex I protein and activity. This is a core function.
    supported_by:
    - reference_id: PMID:19752196
      supporting_text: >-
        huInd1, that is critically required for the assembly of complex I
- term:
    id: GO:0016226
    label: iron-sulfur cluster assembly
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  review:
    summary: >-
      Phylogenetic (IBA) assignment of iron-sulfur cluster assembly. NUBPL binds a
      [4Fe-4S] cluster and delivers Fe-S cofactor(s) to Complex I subunits, so it
      participates in the Fe-S maturation of the Complex I assembly pathway.
    action: ACCEPT
    reason: >-
      Consistent with the family (Mrp/NBP35 Fe-S carrier proteins) and with the
      experimentally demonstrated Fe-S delivery role toward Complex I (PMID:19752196).
      This is a core function, complementary to Complex I assembly.
    supported_by:
    - reference_id: PMID:19752196
      supporting_text: >-
        a possible role in the delivery of one or more Fe/S clusters to complex I subunits
- term:
    id: GO:0051539
    label: 4 iron, 4 sulfur cluster binding
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: enables
  review:
    summary: >-
      Phylogenetic (IBA) assignment of [4Fe-4S] cluster binding, the core molecular
      function. NUBPL binds one [4Fe-4S] cluster via a conserved CXXC motif.
    action: ACCEPT
    reason: >-
      Directly and independently supported by experimental IDA (PMID:19752196) and by the
      UniProt COFACTOR annotation ("Binds 1 [4Fe-4S] cluster"). This is a genuine, core MF.
    supported_by:
    - reference_id: file:human/NUBPL/NUBPL-uniprot.txt
      supporting_text: "Note=Binds 1 [4Fe-4S] cluster."
- term:
    id: GO:0005524
    label: ATP binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: enables
  review:
    summary: >-
      InterPro/keyword-based ATP binding. NUBPL is a Mrp/NBP35 P-loop NTPase with a
      Walker-A/P-loop motif and an annotated ATP-binding site (residues 75-82).
    action: KEEP_AS_NON_CORE
    reason: >-
      The ATP-binding P-loop is a real, conserved feature of the family, but nucleotide
      binding is ancillary to NUBPL's defining function (Fe-S cluster binding and delivery
      to Complex I). No experimental characterization of ATP hydrolysis by human NUBPL is
      available, so it is retained as a non-core feature-based annotation.
    propagation_review:
      root_cause: NO_FAILURE_NON_CORE
      failure_modes:
      - GRANULARITY_MISMATCH
    supported_by:
    - reference_id: file:human/NUBPL/NUBPL-uniprot.txt
      supporting_text: /ligand="ATP"
- term:
    id: GO:0005739
    label: mitochondrion
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  review:
    summary: >-
      UniProt Subcellular Location keyword mapping to mitochondrion. Correct but generic
      relative to the specific mitochondrial matrix localization.
    action: MARK_AS_OVER_ANNOTATED
    reason: >-
      Duplicates the experimentally supported mitochondrion localization at a broad
      granularity; the matrix-specific annotation is preferred as the core localization.
    propagation_review:
      root_cause: NO_FAILURE_NON_CORE
      failure_modes:
      - GRANULARITY_MISMATCH
    supported_by:
    - reference_id: file:human/NUBPL/NUBPL-uniprot.txt
      supporting_text: "SUBCELLULAR LOCATION: Mitochondrion"
- term:
    id: GO:0005759
    label: mitochondrial matrix
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: located_in
  review:
    summary: >-
      ARBA machine-learning assignment to mitochondrial matrix. Consistent with a
      matrix-soluble assembly factor that acts on the peripheral (matrix) arm of Complex I
      and with the Reactome TAS matrix annotations.
    action: ACCEPT
    reason: >-
      NUBPL is a soluble mitochondrial protein imported via a cleavable transit peptide,
      and its Complex I substrate subunits (NDUFS1, NDUFV1) mature in the matrix. The
      matrix is the most specific well-supported localization.
    propagation_review:
      root_cause: NO_FAILURE_CORE
    supported_by:
    - reference_id: file:human/NUBPL/NUBPL-uniprot.txt
      supporting_text: "TRANSIT         1..38"
- term:
    id: GO:0016226
    label: iron-sulfur cluster assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: involved_in
  review:
    summary: >-
      InterPro-based iron-sulfur cluster assembly, redundant with the IBA assignment of
      the same term. Core biological process for this Fe-S carrier protein.
    action: ACCEPT
    reason: >-
      Same conclusion as the IBA annotation of GO:0016226; supported by the [4Fe-4S]
      binding and delivery role toward Complex I.
    propagation_review:
      root_cause: NO_FAILURE_CORE
    supported_by:
    - reference_id: PMID:19752196
      supporting_text: >-
        a possible role in the delivery of one or more Fe/S clusters to complex I subunits
- term:
    id: GO:0032981
    label: mitochondrial respiratory chain complex I assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: involved_in
  review:
    summary: >-
      ARBA machine-learning assignment of Complex I assembly, redundant with the IBA and
      IMP annotations of the same term. Core biological process.
    action: ACCEPT
    reason: >-
      Same core conclusion as the experimental IMP (PMID:19752196) and IBA annotations.
    propagation_review:
      root_cause: NO_FAILURE_CORE
    supported_by:
    - reference_id: PMID:19752196
      supporting_text: >-
        huInd1, that is critically required for the assembly of complex I
- term:
    id: GO:0051536
    label: iron-sulfur cluster binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: enables
  review:
    summary: >-
      InterPro-based generic iron-sulfur cluster binding. This is the parent of the
      specific [4Fe-4S] cluster binding (GO:0051539) that is experimentally supported.
    action: MARK_AS_OVER_ANNOTATED
    reason: >-
      NUBPL binds specifically a [4Fe-4S] cluster (UniProt COFACTOR; IDA PMID:19752196),
      which is captured by the more precise GO:0051539. The broad parent term is
      uninformative and redundant relative to the specific child.
    propagation_review:
      root_cause: NO_FAILURE_NON_CORE
      failure_modes:
      - GRANULARITY_MISMATCH
    supported_by:
    - reference_id: file:human/NUBPL/NUBPL-uniprot.txt
      supporting_text: "Note=Binds 1 [4Fe-4S] cluster."
- term:
    id: GO:0140663
    label: ATP-dependent FeS chaperone activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: enables
  review:
    summary: >-
      InterPro-based ATP-dependent FeS chaperone activity, defined as "binding to and
      delivering metal ions to a target protein, driven by ATP hydrolysis." This is the
      most specific molecular-function term for NUBPL's role as an Fe-S carrier that
      delivers its [4Fe-4S] cluster to Complex I subunits.
    action: ACCEPT
    reason: >-
      Matches the experimentally supported function: NUBPL binds a [4Fe-4S] cluster and
      delivers Fe-S cofactor(s) to Complex I subunits (PMID:19752196), and belongs to the
      ATP-binding Mrp/NBP35 carrier family. This captures the carrier/delivery molecular
      activity better than bare Fe-S binding and is retained as core.
    propagation_review:
      root_cause: NO_FAILURE_CORE
    supported_by:
    - reference_id: PMID:19752196
      supporting_text: >-
        a possible role in the delivery of one or more Fe/S clusters to complex I subunits
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:32296183
  qualifier: enables
  review:
    summary: >-
      Bare "protein binding" from the HuRI binary (Y2H) interactome, capturing NUBPL
      interactions with NUBP2 (Q9Y5Y2) and a KDM8/JMJD5 isoform (Q8N371-3). Uninformative
      as a molecular function.
    action: MARK_AS_OVER_ANNOTATED
    reason: >-
      GO:0005515 conveys no specific function. The high-throughput binary interactions do
      not establish a defined mitochondrial molecular activity for NUBPL; per policy an
      experimental IPI is retained but flagged as over-annotated rather than removed.
    supported_by:
    - reference_id: file:human/NUBPL/NUBPL-uniprot.txt
      supporting_text: "Q8TB37; Q9Y5Y2: NUBP2; NbExp=6"
- term:
    id: GO:0005739
    label: mitochondrion
  evidence_type: IDA
  original_reference_id: GO_REF:0000052
  qualifier: located_in
  review:
    summary: >-
      Immunofluorescence-based (HPA, IDA) localization to mitochondrion. Directly
      supports mitochondrial localization of NUBPL.
    action: ACCEPT
    reason: >-
      Experimental (IDA) mitochondrial localization consistent with the UniProt
      subcellular location and the more specific matrix annotations. Retained; the matrix
      term is the finer-grained core localization.
    supported_by:
    - reference_id: file:human/NUBPL/NUBPL-uniprot.txt
      supporting_text: "SUBCELLULAR LOCATION: Mitochondrion"
- term:
    id: GO:0005739
    label: mitochondrion
  evidence_type: HTP
  original_reference_id: PMID:34800366
  qualifier: located_in
  review:
    summary: >-
      High-throughput (HTP) mitochondrial-proteome localization to mitochondrion from a
      quantitative high-confidence human mitochondrial proteome study.
    action: KEEP_AS_NON_CORE
    reason: >-
      Corroborating high-throughput evidence for mitochondrial localization; consistent
      with the IDA annotations but non-core relative to the specific matrix localization.
    supported_by:
    - reference_id: file:human/NUBPL/NUBPL-uniprot.txt
      supporting_text: "SUBCELLULAR LOCATION: Mitochondrion"
- term:
    id: GO:0005759
    label: mitochondrial matrix
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-5690023
  qualifier: located_in
  review:
    summary: >-
      Reactome TAS placing NUBPL in the mitochondrial matrix, in the reaction where NUBPL
      binds the [4Fe-4S] cluster. Consistent with a matrix-soluble assembly factor.
    action: ACCEPT
    reason: >-
      Mitochondrial matrix is the specific, well-supported localization for this soluble
      assembly factor whose Complex I substrate subunits mature in the matrix.
    supported_by:
    - reference_id: file:human/NUBPL/NUBPL-uniprot.txt
      supporting_text: "TRANSIT         1..38"
- term:
    id: GO:0005759
    label: mitochondrial matrix
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-6788523
  qualifier: located_in
  review:
    summary: >-
      Reactome TAS matrix localization from the reaction "NUBPL transfers 4Fe-4S to
      Complex I subunits." Duplicate of the same matrix localization.
    action: ACCEPT
    reason: >-
      Same well-supported matrix localization; consistent with NUBPL's Fe-S delivery role.
    supported_by:
    - reference_id: file:human/NUBPL/NUBPL-uniprot.txt
      supporting_text: "TRANSIT         1..38"
- term:
    id: GO:0005759
    label: mitochondrial matrix
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-6788556
  qualifier: located_in
  review:
    summary: >-
      Reactome TAS matrix localization from the reaction "NUBPL transfers 4Fe-4S to
      NDUFV1, V2." Duplicate of the same matrix localization.
    action: ACCEPT
    reason: >-
      Same well-supported matrix localization; consistent with NUBPL's Fe-S delivery role.
    supported_by:
    - reference_id: file:human/NUBPL/NUBPL-uniprot.txt
      supporting_text: "TRANSIT         1..38"
- term:
    id: GO:0005739
    label: mitochondrion
  evidence_type: IDA
  original_reference_id: PMID:19752196
  qualifier: located_in
  review:
    summary: >-
      Experimental (IDA) mitochondrial localization from the primary characterization of
      huInd1 (Sheftel et al. 2009), which established the subcellular location of NUBPL.
    action: ACCEPT
    reason: >-
      This is the source experimental IDA underlying the UniProt "Mitochondrion"
      subcellular location (ECO:0000269|PubMed:19752196). Core mitochondrial localization.
    supported_by:
    - reference_id: file:human/NUBPL/NUBPL-uniprot.txt
      supporting_text: >-
        SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:19752196}
- term:
    id: GO:0007005
    label: mitochondrion organization
  evidence_type: IMP
  original_reference_id: PMID:19752196
  qualifier: involved_in
  review:
    summary: >-
      IMP annotation based on the altered mitochondrial morphology observed upon huInd1
      knockdown. This is a downstream, pleiotropic consequence of loss of Complex I rather
      than a direct core function of NUBPL.
    action: KEEP_AS_NON_CORE
    reason: >-
      NUBPL's direct role is Fe-S cluster binding and delivery for Complex I assembly; the
      "alteration of mitochondrial morphology" reported on knockdown is a secondary effect
      of respiratory-chain dysfunction, so this BP is retained as non-core rather than a
      defining function.
    supported_by:
    - reference_id: PMID:19752196
      supporting_text: >-
        remodeling of respiratory supercomplexes, and alteration of mitochondrial
        morphology
- term:
    id: GO:0032981
    label: mitochondrial respiratory chain complex I assembly
  evidence_type: IMP
  original_reference_id: PMID:19752196
  qualifier: involved_in
  review:
    summary: >-
      Experimental (IMP) evidence that NUBPL/huInd1 is required for Complex I assembly:
      RNAi knockdown caused strong decreases in Complex I protein and activity. This is a
      core biological process.
    action: ACCEPT
    reason: >-
      This IMP is the primary experimental basis for NUBPL's function as a Complex I
      assembly factor and underlies the disease association (MC1DN21).
    supported_by:
    - reference_id: PMID:19752196
      supporting_text: >-
        Knockdown of huInd1 in HeLa cells by RNA interference technology led to strong
        decreases in complex I protein and activity levels
- term:
    id: GO:0051539
    label: 4 iron, 4 sulfur cluster binding
  evidence_type: IDA
  original_reference_id: PMID:19752196
  qualifier: enables
  review:
    summary: >-
      Experimental (IDA) evidence that NUBPL/huInd1 binds an Fe-S cluster via a conserved
      CXXC motif. This is the core molecular function, matching the UniProt COFACTOR
      annotation of one [4Fe-4S] cluster.
    action: ACCEPT
    reason: >-
      Directly demonstrated cofactor binding; the CXXC-motif cysteines (Cys-244/Cys-247)
      are required for Complex I assembly, tying the [4Fe-4S] binding to function.
    supported_by:
    - reference_id: PMID:19752196
      supporting_text: >-
        huInd1 can bind an Fe/S cluster via a conserved CXXC motif in a labile fashion
core_functions:
- description: >-
    Binds a single [4Fe-4S] cluster via a conserved CXXC motif and acts as an
    ATP-binding Mrp/NBP35-family Fe-S carrier that delivers Fe-S cofactor(s) to
    respiratory Complex I subunits during their maturation in the mitochondrial matrix.
  molecular_function:
    id: GO:0140663
    label: ATP-dependent FeS chaperone activity
  directly_involved_in:
  - id: GO:0032981
    label: mitochondrial respiratory chain complex I assembly
  - id: GO:0016226
    label: iron-sulfur cluster assembly
  locations:
  - id: GO:0005759
    label: mitochondrial matrix
  supported_by:
  - reference_id: PMID:19752196
    supporting_text: >-
      a possible role in the delivery of one or more Fe/S clusters to complex I subunits
  - reference_id: file:human/NUBPL/NUBPL-uniprot.txt
    supporting_text: "Note=Binds 1 [4Fe-4S] cluster."
- description: >-
    Binds a [4Fe-4S] iron-sulfur cluster, the cofactor that NUBPL carries and transfers;
    the cluster is coordinated by a conserved CXXC motif (Cys-244/Cys-247) required for
    Complex I assembly.
  molecular_function:
    id: GO:0051539
    label: 4 iron, 4 sulfur cluster binding
  directly_involved_in:
  - id: GO:0032981
    label: mitochondrial respiratory chain complex I assembly
  locations:
  - id: GO:0005759
    label: mitochondrial matrix
  supported_by:
  - reference_id: PMID:19752196
    supporting_text: >-
      huInd1 can bind an Fe/S cluster via a conserved CXXC motif in a labile fashion
  - reference_id: file:human/NUBPL/NUBPL-uniprot.txt
    supporting_text: "Note=Binds 1 [4Fe-4S] cluster."
references:
- id: GO_REF:0000002
  title: Gene Ontology annotation through association of InterPro records with GO
    terms
  findings: []
  reference_review:
    relevance: MEDIUM
    correctness: VERIFIED
    review_notes: >-
      InterPro2GO pipeline. Correctly recovers family-level MF/BP terms (ATP binding,
      Fe-S binding, ATP-dependent FeS chaperone activity, Fe-S cluster assembly). The
      generic parent GO:0051536 is redundant with the specific GO:0051539.
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: >-
      PAN-GO/IBA pipeline; correctly recovers the core functions (4Fe-4S binding,
      Complex I assembly, Fe-S cluster assembly, mitochondrial localization).
- id: GO_REF:0000044
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location
    vocabulary mapping, accompanied by conservative changes to GO terms applied by
    UniProt
  findings: []
  reference_review:
    relevance: MEDIUM
    correctness: VERIFIED
    review_notes: >-
      SubCell keyword mapping to mitochondrion; correct but generic relative to the
      matrix-specific localization.
- id: GO_REF:0000052
  title: Gene Ontology annotation based on curation of immunofluorescence data
  findings: []
  reference_review:
    relevance: MEDIUM
    correctness: VERIFIED
    review_notes: >-
      HPA immunofluorescence supporting mitochondrial localization (IDA).
- id: GO_REF:0000117
  title: Electronic Gene Ontology annotations created by ARBA machine learning models
  findings: []
  reference_review:
    relevance: MEDIUM
    correctness: VERIFIED
    review_notes: >-
      ARBA machine-learning annotations for mitochondrial matrix localization and
      Complex I assembly; consistent with experimental and Reactome evidence.
- id: PMID:19752196
  title: Human ind1, an iron-sulfur cluster assembly factor for respiratory complex
    I.
  findings:
  - statement: >-
      huInd1 (NUBPL) is a mitochondrial P-loop NTPase critically required for the
      assembly of respiratory Complex I; knockdown decreases Complex I protein and
      activity and loss of peripheral-arm subunits.
    reference_section_type: ABSTRACT
    supporting_text: >-
      huInd1, that is critically required for the assembly of complex I. huInd1 can
      bind an Fe/S cluster via a conserved CXXC motif in a labile fashion.
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: >-
      Primary characterization of human NUBPL/IND1. Only the abstract is cached
      (full_text_available: false); all supporting_text quotes are verbatim from the
      cached abstract. Establishes Fe-S binding, Complex I assembly requirement, and
      mitochondrial localization (source of the UniProt ECO:0000269 annotations).
- id: PMID:32296183
  title: A reference map of the human binary protein interactome.
  findings:
  - statement: >-
      Large-scale binary (Y2H) interactome map (HuRI); the source of the NUBPL protein
      binding (IPI) annotations to NUBP2 and a KDM8 isoform.
    reference_section_type: ABSTRACT
    supporting_text: >-
      we present a human 'all-by-all' reference interactome map of human binary protein
      interactions, or 'HuRI'.
  reference_review:
    relevance: LOW
    correctness: VERIFIED
    review_notes: >-
      High-throughput binary interactome; supports the existence of interactions but not
      a specific molecular function for NUBPL. Underlies the over-annotated bare
      "protein binding" (GO:0005515) IPI rows.
- id: PMID:34800366
  title: Quantitative high-confidence human mitochondrial proteome and its dynamics
    in cellular context.
  findings:
  - statement: >-
      High-confidence human mitochondrial proteome; corroborates mitochondrial
      localization of NUBPL (HTP).
    reference_section_type: ABSTRACT
    supporting_text: >-
      Quantitative high-confidence human mitochondrial proteome and its dynamics in
      cellular context.
  reference_review:
    relevance: MEDIUM
    correctness: VERIFIED
    review_notes: >-
      High-throughput mitochondrial proteome; supports mitochondrial localization but is
      non-core relative to the specific matrix annotation.
- id: Reactome:R-HSA-5690023
  title: NUBPL binds 4Fe-4S
  findings: []
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: >-
      Reactome reaction describing NUBPL binding the [4Fe-4S] cofactor in the
      mitochondrial matrix; consistent with UniProt COFACTOR and the primary literature.
- id: Reactome:R-HSA-6788523
  title: NUBPL transfers 4Fe-4S to Complex I subunits
  findings: []
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: >-
      Reactome reaction describing NUBPL transferring the [4Fe-4S] cluster to Complex I
      subunits (NDUFS1/S2/S7/S8); supports the Fe-S delivery / Complex I assembly role.
- id: Reactome:R-HSA-6788556
  title: NUBPL transfers 4Fe-4S to NDUFV1, V2
  findings: []
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: >-
      Reactome reaction describing NUBPL transferring the [4Fe-4S] cluster to NDUFV1/V2;
      supports the Fe-S delivery / Complex I assembly role.