PSMB5 encodes proteasome subunit beta type-5, the constitutive beta5 catalytic subunit of the human 20S proteasome core. After removal of its N-terminal propeptide, the mature chain begins with Thr60, the nucleophilic active-site residue that supports chymotrypsin-like threonine endopeptidase activity. PSMB5 therefore carries catalytic protease function as a member of the 20S/26S proteasome, in contrast to non-catalytic structural subunits such as PSMA1.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process | IBA GO_REF:0000033 | ACCEPT | Summary: proteasome-mediated ubiquitin-dependent protein catabolic process captures the proteasome-mediated protein catabolism process supported for this subunit as part of the proteasome. Reason: The gene product is a core 20S proteasome subunit, so proteasomal protein catabolism is an appropriate core biological-process context. |
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: nucleus is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | IBA GO_REF:0000033 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0004175 endopeptidase activity | IBA GO_REF:0000033 | MODIFY | Summary: endopeptidase activity is directionally related to the proteasome role but is less specific than the supported proteasome term. Reason: Replace the broad annotation with threonine-type endopeptidase activity, which better captures the gene product role supported by proteasome literature and existing specific GOA annotations. Proposed replacements: threonine-type endopeptidase activity Supporting Evidence: file:human/PSMB5/PSMB5-deep-research-falcon.md Within the 20S Ξ² rings, Ξ²1, Ξ²2, and Ξ²5 are the three catalytically active Ξ²-subunits. Ξ²5 (PSMB5) is assigned the CT-L activity, which preferentially cleaves peptide bonds after hydrophobic residues. |
| GO:0019774 proteasome core complex, beta-subunit complex | IBA GO_REF:0000033 | ACCEPT | Summary: proteasome core complex, beta-subunit complex is the appropriate core-complex membership annotation for PSMB5. Reason: Structural studies and UniProt summaries place the protein in the 20S proteasome core; this complex-membership annotation is central to the gene product role. Supporting Evidence: file:human/PSMB5/PSMB5-deep-research-falcon.md In the standard/constitutive proteasome, Ξ²5 is one of three catalytically active Ξ²-subunits (Ξ²1, Ξ²2, Ξ²5) and is responsible for the chymotrypsin-like (CT-L) activity. |
| GO:0004298 threonine-type endopeptidase activity | IEA GO_REF:0000002 | ACCEPT | Summary: PSMB5 is the beta5 catalytic subunit of the constitutive 20S proteasome and carries the threonine-type endopeptidase active site. Reason: The mature PSMB5 chain begins at Thr60, annotated as the nucleophilic active-site residue, and structural/activity studies support beta5 chymotrypsin-like proteasome activity. Supporting Evidence: file:human/PSMB5/PSMB5-deep-research-falcon.md Proteasome catalytic Ξ² subunits operate via an N-terminal nucleophile mechanism centered on the N-terminal threonine (Thr1). Mechanistically, Thr1 attacks the scissile peptide bond carbonyl to form an acyl-enzyme intermediate, which is subsequently hydrolyzed to release products and regenerate the catalytic threonine. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | ACCEPT | Summary: nucleus is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: cytoplasm is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0005839 proteasome core complex | IEA GO_REF:0000120 | ACCEPT | Summary: proteasome core complex is the appropriate core-complex membership annotation for PSMB5. Reason: Structural studies and UniProt summaries place the protein in the 20S proteasome core; this complex-membership annotation is central to the gene product role. Supporting Evidence: file:human/PSMB5/PSMB5-deep-research-falcon.md The target gene PSMB5 (proteasome 20S subunit beta 5) encodes the constitutive catalytic Ξ²5 subunit of the 20S proteasome core particle (CP), which forms the proteolytic core of the 26S proteasome. |
| GO:0010498 proteasomal protein catabolic process | IEA GO_REF:0000117 | ACCEPT | Summary: proteasomal protein catabolic process captures the proteasome-mediated protein catabolism process supported for this subunit as part of the proteasome. Reason: The gene product is a core 20S proteasome subunit, so proteasomal protein catabolism is an appropriate core biological-process context. Supporting Evidence: file:human/PSMB5/PSMB5-deep-research-falcon.md The eukaryotic 26S proteasome is the principal executor of ubiquitinβproteasome system (UPS) protein degradation. |
| GO:0030163 protein catabolic process | IEA GO_REF:0000002 | MODIFY | Summary: protein catabolic process is directionally related to the proteasome role but is less specific than the supported proteasome term. Reason: Replace the broad annotation with proteasomal protein catabolic process, which better captures the gene product role supported by proteasome literature and existing specific GOA annotations. Proposed replacements: proteasomal protein catabolic process |
| GO:0005515 protein binding | IPI PMID:14733938 Protein-protein interactions among human 20S proteasome subu... | MARK AS OVER ANNOTATED | Summary: The interaction evidence may show physical association, but generic protein binding is too low-information for the reviewed function of this proteasome subunit. Reason: For proteasome subunits, the informative annotations are catalytic activity, structural constituent activity, complex membership, and proteasomal protein catabolism; generic protein binding should not be treated as a core function. |
| GO:0005515 protein binding | IPI PMID:15231747 A protein interaction framework for human mRNA degradation. | MARK AS OVER ANNOTATED | Summary: The interaction evidence may show physical association, but generic protein binding is too low-information for the reviewed function of this proteasome subunit. Reason: For proteasome subunits, the informative annotations are catalytic activity, structural constituent activity, complex membership, and proteasomal protein catabolism; generic protein binding should not be treated as a core function. |
| GO:0005515 protein binding | IPI PMID:17948026 The proteasome maturation protein POMP facilitates major ste... | MARK AS OVER ANNOTATED | Summary: The interaction evidence may show physical association, but generic protein binding is too low-information for the reviewed function of this proteasome subunit. Reason: For proteasome subunits, the informative annotations are catalytic activity, structural constituent activity, complex membership, and proteasomal protein catabolism; generic protein binding should not be treated as a core function. |
| GO:0005515 protein binding | IPI PMID:20723761 Immunoproteasomes preserve protein homeostasis upon interfer... | MARK AS OVER ANNOTATED | Summary: The interaction evidence may show physical association, but generic protein binding is too low-information for the reviewed function of this proteasome subunit. Reason: For proteasome subunits, the informative annotations are catalytic activity, structural constituent activity, complex membership, and proteasomal protein catabolism; generic protein binding should not be treated as a core function. |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | MARK AS OVER ANNOTATED | Summary: The interaction evidence may show physical association, but generic protein binding is too low-information for the reviewed function of this proteasome subunit. Reason: For proteasome subunits, the informative annotations are catalytic activity, structural constituent activity, complex membership, and proteasomal protein catabolism; generic protein binding should not be treated as a core function. |
| GO:0005515 protein binding | IPI PMID:31473102 Characterization of Fully Recombinant Human 20S and 20S-PA20... | MARK AS OVER ANNOTATED | Summary: The interaction evidence may show physical association, but generic protein binding is too low-information for the reviewed function of this proteasome subunit. Reason: For proteasome subunits, the informative annotations are catalytic activity, structural constituent activity, complex membership, and proteasomal protein catabolism; generic protein binding should not be treated as a core function. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: The interaction evidence may show physical association, but generic protein binding is too low-information for the reviewed function of this proteasome subunit. Reason: For proteasome subunits, the informative annotations are catalytic activity, structural constituent activity, complex membership, and proteasomal protein catabolism; generic protein binding should not be treated as a core function. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MARK AS OVER ANNOTATED | Summary: The interaction evidence may show physical association, but generic protein binding is too low-information for the reviewed function of this proteasome subunit. Reason: For proteasome subunits, the informative annotations are catalytic activity, structural constituent activity, complex membership, and proteasomal protein catabolism; generic protein binding should not be treated as a core function. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | MARK AS OVER ANNOTATED | Summary: The interaction evidence may show physical association, but generic protein binding is too low-information for the reviewed function of this proteasome subunit. Reason: For proteasome subunits, the informative annotations are catalytic activity, structural constituent activity, complex membership, and proteasomal protein catabolism; generic protein binding should not be treated as a core function. |
| GO:0005515 protein binding | IPI PMID:35858375 Structural insights into the human PA28-20S proteasome enabl... | MARK AS OVER ANNOTATED | Summary: The interaction evidence may show physical association, but generic protein binding is too low-information for the reviewed function of this proteasome subunit. Reason: For proteasome subunits, the informative annotations are catalytic activity, structural constituent activity, complex membership, and proteasomal protein catabolism; generic protein binding should not be treated as a core function. |
| GO:0005515 protein binding | IPI PMID:40205054 Multimodal cell maps as a foundation for structural and func... | MARK AS OVER ANNOTATED | Summary: The interaction evidence may show physical association, but generic protein binding is too low-information for the reviewed function of this proteasome subunit. Reason: For proteasome subunits, the informative annotations are catalytic activity, structural constituent activity, complex membership, and proteasomal protein catabolism; generic protein binding should not be treated as a core function. |
| GO:0008233 peptidase activity | IEA GO_REF:0000120 | MODIFY | Summary: peptidase activity is directionally related to the proteasome role but is less specific than the supported proteasome term. Reason: Replace the broad annotation with threonine-type endopeptidase activity, which better captures the gene product role supported by proteasome literature and existing specific GOA annotations. Proposed replacements: threonine-type endopeptidase activity |
| GO:0004298 threonine-type endopeptidase activity | IDA PMID:27493187 The inhibition mechanism of human 20S proteasomes enables ne... | ACCEPT | Summary: PSMB5 is the beta5 catalytic subunit of the constitutive 20S proteasome and carries the threonine-type endopeptidase active site. Reason: The mature PSMB5 chain begins at Thr60, annotated as the nucleophilic active-site residue, and structural/activity studies support beta5 chymotrypsin-like proteasome activity. Supporting Evidence: file:human/PSMB5/PSMB5-deep-research-falcon.md Ξ²5 (PSMB5) is assigned the CT-L activity, which preferentially cleaves peptide bonds after hydrophobic residues. |
| GO:0000502 proteasome complex | NAS PMID:29636472 Structural mechanism for nucleotide-driven remodeling of the... | KEEP AS NON CORE | Summary: proteasome complex is true complex membership but broader or more context-specific than the core 20S subunit identity. Reason: The core review should emphasize the specific 20S core subcomplex membership; broader proteasome or spermatoproteasome membership can be retained as non-core context. |
| GO:0000502 proteasome complex | NAS PMID:33729481 Proteasome in action: substrate degradation by the 26S prote... | KEEP AS NON CORE | Summary: proteasome complex is true complex membership but broader or more context-specific than the core 20S subunit identity. Reason: The core review should emphasize the specific 20S core subcomplex membership; broader proteasome or spermatoproteasome membership can be retained as non-core context. |
| GO:0000502 proteasome complex | NAS PMID:37228199 An abundance of free regulatory (19S) proteasome particles r... | KEEP AS NON CORE | Summary: proteasome complex is true complex membership but broader or more context-specific than the core 20S subunit identity. Reason: The core review should emphasize the specific 20S core subcomplex membership; broader proteasome or spermatoproteasome membership can be retained as non-core context. |
| GO:0005829 cytosol | NAS PMID:12032076 Properties of the hybrid form of the 26S proteasome containi... | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. Supporting Evidence: file:human/PSMB5/PSMB5-deep-research-falcon.md The active sites are located on the inner surface of the 20S core, sequestered in the central catalytic chamber; access is controlled by Ξ±-ring gating and by association with regulatory particles such as the 19S cap (forming the 26S proteasome). |
| GO:0008021 synaptic vesicle | NAS PMID:37228199 An abundance of free regulatory (19S) proteasome particles r... | KEEP AS NON CORE | Summary: synaptic vesicle is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0010498 proteasomal protein catabolic process | NAS PMID:33729481 Proteasome in action: substrate degradation by the 26S prote... | ACCEPT | Summary: proteasomal protein catabolic process captures the proteasome-mediated protein catabolism process supported for this subunit as part of the proteasome. Reason: The gene product is a core 20S proteasome subunit, so proteasomal protein catabolism is an appropriate core biological-process context. |
| GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process | NAS PMID:19489727 Recognition and processing of ubiquitin-protein conjugates b... | ACCEPT | Summary: proteasome-mediated ubiquitin-dependent protein catabolic process captures the proteasome-mediated protein catabolism process supported for this subunit as part of the proteasome. Reason: The gene product is a core 20S proteasome subunit, so proteasomal protein catabolism is an appropriate core biological-process context. |
| GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process | NAS PMID:33729481 Proteasome in action: substrate degradation by the 26S prote... | ACCEPT | Summary: proteasome-mediated ubiquitin-dependent protein catabolic process captures the proteasome-mediated protein catabolism process supported for this subunit as part of the proteasome. Reason: The gene product is a core 20S proteasome subunit, so proteasomal protein catabolism is an appropriate core biological-process context. |
| GO:0061136 regulation of proteasomal protein catabolic process | NAS PMID:12032076 Properties of the hybrid form of the 26S proteasome containi... | MARK AS OVER ANNOTATED | Summary: regulation of proteasomal protein catabolic process is a pathway- or physiology-level consequence of proteasome activity rather than a specific function of PSMB5. Reason: These annotations are largely projected from whole proteasome complexes or specialized proteasome variants; they should not be interpreted as individual subunit core functions. |
| GO:0000502 proteasome complex | NAS PMID:32134919 Cryo-EM structures of the human PA200 and PA200-20S complex ... | KEEP AS NON CORE | Summary: proteasome complex is true complex membership but broader or more context-specific than the core 20S subunit identity. Reason: The core review should emphasize the specific 20S core subcomplex membership; broader proteasome or spermatoproteasome membership can be retained as non-core context. |
| GO:0000502 proteasome complex | IPI PMID:34702852 The 20S as a stand-alone proteasome in cells can degrade the... | KEEP AS NON CORE | Summary: proteasome complex is true complex membership but broader or more context-specific than the core 20S subunit identity. Reason: The core review should emphasize the specific 20S core subcomplex membership; broader proteasome or spermatoproteasome membership can be retained as non-core context. |
| GO:0000502 proteasome complex | IPI PMID:35714770 Proteasome activator 28Ξ³ (PA28Ξ³) allosterically activates tr... | KEEP AS NON CORE | Summary: proteasome complex is true complex membership but broader or more context-specific than the core 20S subunit identity. Reason: The core review should emphasize the specific 20S core subcomplex membership; broader proteasome or spermatoproteasome membership can be retained as non-core context. |
| GO:0000502 proteasome complex | IPI PMID:35858375 Structural insights into the human PA28-20S proteasome enabl... | KEEP AS NON CORE | Summary: proteasome complex is true complex membership but broader or more context-specific than the core 20S subunit identity. Reason: The core review should emphasize the specific 20S core subcomplex membership; broader proteasome or spermatoproteasome membership can be retained as non-core context. |
| GO:0005634 nucleus | NAS PMID:32134919 Cryo-EM structures of the human PA200 and PA200-20S complex ... | ACCEPT | Summary: nucleus is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005634 nucleus | NAS PMID:35858375 Structural insights into the human PA28-20S proteasome enabl... | ACCEPT | Summary: nucleus is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005737 cytoplasm | NAS PMID:35858375 Structural insights into the human PA28-20S proteasome enabl... | KEEP AS NON CORE | Summary: cytoplasm is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0005839 proteasome core complex | IPI PMID:34702852 The 20S as a stand-alone proteasome in cells can degrade the... | ACCEPT | Summary: proteasome core complex is the appropriate core-complex membership annotation for PSMB5. Reason: Structural studies and UniProt summaries place the protein in the 20S proteasome core; this complex-membership annotation is central to the gene product role. |
| GO:0006281 DNA repair | NAS PMID:32134919 Cryo-EM structures of the human PA200 and PA200-20S complex ... | MARK AS OVER ANNOTATED | Summary: DNA repair is a pathway- or physiology-level consequence of proteasome activity rather than a specific function of PSMB5. Reason: These annotations are largely projected from whole proteasome complexes or specialized proteasome variants; they should not be interpreted as individual subunit core functions. |
| GO:0006915 apoptotic process | NAS PMID:32935661 Role of oncogenic REGΞ³ in cancer. | MARK AS OVER ANNOTATED | Summary: apoptotic process is a pathway- or physiology-level consequence of proteasome activity rather than a specific function of PSMB5. Reason: These annotations are largely projected from whole proteasome complexes or specialized proteasome variants; they should not be interpreted as individual subunit core functions. |
| GO:0006974 DNA damage response | NAS PMID:32134919 Cryo-EM structures of the human PA200 and PA200-20S complex ... | MARK AS OVER ANNOTATED | Summary: DNA damage response is a pathway- or physiology-level consequence of proteasome activity rather than a specific function of PSMB5. Reason: These annotations are largely projected from whole proteasome complexes or specialized proteasome variants; they should not be interpreted as individual subunit core functions. |
| GO:0006979 response to oxidative stress | IDA PMID:34702852 The 20S as a stand-alone proteasome in cells can degrade the... | MARK AS OVER ANNOTATED | Summary: response to oxidative stress is a pathway- or physiology-level consequence of proteasome activity rather than a specific function of PSMB5. Reason: These annotations are largely projected from whole proteasome complexes or specialized proteasome variants; they should not be interpreted as individual subunit core functions. |
| GO:0006979 response to oxidative stress | NAS PMID:35858375 Structural insights into the human PA28-20S proteasome enabl... | MARK AS OVER ANNOTATED | Summary: response to oxidative stress is a pathway- or physiology-level consequence of proteasome activity rather than a specific function of PSMB5. Reason: These annotations are largely projected from whole proteasome complexes or specialized proteasome variants; they should not be interpreted as individual subunit core functions. |
| GO:0007283 spermatogenesis | NAS PMID:23706739 Acetylation-mediated proteasomal degradation of core histone... | MARK AS OVER ANNOTATED | Summary: spermatogenesis is a pathway- or physiology-level consequence of proteasome activity rather than a specific function of PSMB5. Reason: These annotations are largely projected from whole proteasome complexes or specialized proteasome variants; they should not be interpreted as individual subunit core functions. |
| GO:0010498 proteasomal protein catabolic process | NAS PMID:23706739 Acetylation-mediated proteasomal degradation of core histone... | ACCEPT | Summary: proteasomal protein catabolic process captures the proteasome-mediated protein catabolism process supported for this subunit as part of the proteasome. Reason: The gene product is a core 20S proteasome subunit, so proteasomal protein catabolism is an appropriate core biological-process context. |
| GO:0010498 proteasomal protein catabolic process | IDA PMID:34702852 The 20S as a stand-alone proteasome in cells can degrade the... | ACCEPT | Summary: proteasomal protein catabolic process captures the proteasome-mediated protein catabolism process supported for this subunit as part of the proteasome. Reason: The gene product is a core 20S proteasome subunit, so proteasomal protein catabolism is an appropriate core biological-process context. |
| GO:0010499 proteasomal ubiquitin-independent protein catabolic process | NAS PMID:31473102 Characterization of Fully Recombinant Human 20S and 20S-PA20... | ACCEPT | Summary: proteasomal ubiquitin-independent protein catabolic process captures the proteasome-mediated protein catabolism process supported for this subunit as part of the proteasome. Reason: The gene product is a core 20S proteasome subunit, so proteasomal protein catabolism is an appropriate core biological-process context. |
| GO:0030317 flagellated sperm motility | NAS PMID:23706739 Acetylation-mediated proteasomal degradation of core histone... | MARK AS OVER ANNOTATED | Summary: flagellated sperm motility is a pathway- or physiology-level consequence of proteasome activity rather than a specific function of PSMB5. Reason: These annotations are largely projected from whole proteasome complexes or specialized proteasome variants; they should not be interpreted as individual subunit core functions. |
| GO:0030317 flagellated sperm motility | NAS PMID:27003159 Proteasome activators, PA28Ξ³ and PA200, play indispensable r... | MARK AS OVER ANNOTATED | Summary: flagellated sperm motility is a pathway- or physiology-level consequence of proteasome activity rather than a specific function of PSMB5. Reason: These annotations are largely projected from whole proteasome complexes or specialized proteasome variants; they should not be interpreted as individual subunit core functions. |
| GO:0034515 proteasome storage granule | NAS PMID:31380390 Regulation of Proteasome Activity by (Post-)transcriptional ... | KEEP AS NON CORE | Summary: proteasome storage granule is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process | IDA PMID:34702852 The 20S as a stand-alone proteasome in cells can degrade the... | ACCEPT | Summary: proteasome-mediated ubiquitin-dependent protein catabolic process captures the proteasome-mediated protein catabolism process supported for this subunit as part of the proteasome. Reason: The gene product is a core 20S proteasome subunit, so proteasomal protein catabolism is an appropriate core biological-process context. Supporting Evidence: file:human/PSMB5/PSMB5-deep-research-falcon.md Its 20S core particle is a barrel-like complex of four stacked heptameric rings (Ξ±/Ξ²/Ξ²/Ξ±). The active sites are located on the inner surface of the 20S core, sequestered in the central catalytic chamber. |
| GO:0051321 meiotic cell cycle | NAS PMID:23706739 Acetylation-mediated proteasomal degradation of core histone... | MARK AS OVER ANNOTATED | Summary: meiotic cell cycle is a pathway- or physiology-level consequence of proteasome activity rather than a specific function of PSMB5. Reason: These annotations are largely projected from whole proteasome complexes or specialized proteasome variants; they should not be interpreted as individual subunit core functions. |
| GO:0061136 regulation of proteasomal protein catabolic process | IDA PMID:35714770 Proteasome activator 28Ξ³ (PA28Ξ³) allosterically activates tr... | MARK AS OVER ANNOTATED | Summary: regulation of proteasomal protein catabolic process is a pathway- or physiology-level consequence of proteasome activity rather than a specific function of PSMB5. Reason: These annotations are largely projected from whole proteasome complexes or specialized proteasome variants; they should not be interpreted as individual subunit core functions. |
| GO:0061136 regulation of proteasomal protein catabolic process | NAS PMID:35714770 Proteasome activator 28Ξ³ (PA28Ξ³) allosterically activates tr... | MARK AS OVER ANNOTATED | Summary: regulation of proteasomal protein catabolic process is a pathway- or physiology-level consequence of proteasome activity rather than a specific function of PSMB5. Reason: These annotations are largely projected from whole proteasome complexes or specialized proteasome variants; they should not be interpreted as individual subunit core functions. |
| GO:0071357 cellular response to type I interferon | NAS PMID:31380390 Regulation of Proteasome Activity by (Post-)transcriptional ... | MARK AS OVER ANNOTATED | Summary: cellular response to type I interferon is a pathway- or physiology-level consequence of proteasome activity rather than a specific function of PSMB5. Reason: These annotations are largely projected from whole proteasome complexes or specialized proteasome variants; they should not be interpreted as individual subunit core functions. |
| GO:1990111 spermatoproteasome complex | NAS PMID:35377789 Proteasome complexes experience profound structural and func... | KEEP AS NON CORE | Summary: spermatoproteasome complex is true complex membership but broader or more context-specific than the core 20S subunit identity. Reason: The core review should emphasize the specific 20S core subcomplex membership; broader proteasome or spermatoproteasome membership can be retained as non-core context. |
| GO:2000045 regulation of G1/S transition of mitotic cell cycle | NAS PMID:32935661 Role of oncogenic REGΞ³ in cancer. | MARK AS OVER ANNOTATED | Summary: regulation of G1/S transition of mitotic cell cycle is a pathway- or physiology-level consequence of proteasome activity rather than a specific function of PSMB5. Reason: These annotations are largely projected from whole proteasome complexes or specialized proteasome variants; they should not be interpreted as individual subunit core functions. |
| GO:0005654 nucleoplasm | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: nucleoplasm is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0010499 proteasomal ubiquitin-independent protein catabolic process | TAS Reactome:R-HSA-9912633 | ACCEPT | Summary: proteasomal ubiquitin-independent protein catabolic process captures the proteasome-mediated protein catabolism process supported for this subunit as part of the proteasome. Reason: The gene product is a core 20S proteasome subunit, so proteasomal protein catabolism is an appropriate core biological-process context. |
| GO:0043248 proteasome assembly | TAS Reactome:R-HSA-9907900 | KEEP AS NON CORE | Summary: PSMB5 maturation and incorporation are relevant to proteasome biogenesis, but assembly is not the mature subunit catalytic function. Reason: Retain proteasome assembly as non-core context because PSMB5 has a propeptide and assembly dependencies, while the reviewed core function is beta5 proteolysis in the mature 20S core. Supporting Evidence: file:human/PSMB5/PSMB5-deep-research-falcon.md Catalytic Ξ² subunits are synthesized with N-terminal propeptides that are removed by autolysis after correct assembly. The propeptides are described as being autolyzed between a glycine residue and a threonine residue, yielding the mature catalytic subunit with Thr1 at the N-terminus; Ξ²5 propeptides also contribute to proteasome assembly. |
| GO:0005634 nucleus | EXP PMID:12181345 Clastosome: a subtype of nuclear body enriched in 19S and 20... | ACCEPT | Summary: nucleus is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005737 cytoplasm | EXP PMID:12181345 Clastosome: a subtype of nuclear body enriched in 19S and 20... | KEEP AS NON CORE | Summary: cytoplasm is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0004175 endopeptidase activity | TAS Reactome:R-HSA-9912636 | MODIFY | Summary: endopeptidase activity is directionally related to the proteasome role but is less specific than the supported proteasome term. Reason: Replace the broad annotation with threonine-type endopeptidase activity, which better captures the gene product role supported by proteasome literature and existing specific GOA annotations. Proposed replacements: threonine-type endopeptidase activity |
| GO:0004175 endopeptidase activity | TAS Reactome:R-HSA-9908101 | MODIFY | Summary: endopeptidase activity is directionally related to the proteasome role but is less specific than the supported proteasome term. Reason: Replace the broad annotation with threonine-type endopeptidase activity, which better captures the gene product role supported by proteasome literature and existing specific GOA annotations. Proposed replacements: threonine-type endopeptidase activity |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1168640 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1234159 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1236970 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1504193 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-174105 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-174202 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-174203 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-174255 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-180573 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-180603 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-209061 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-2130282 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-264458 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-353125 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-3640874 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-450466 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-4608855 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-4641256 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-4641260 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5362448 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5387392 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5607724 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5607731 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5610754 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5610758 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5610760 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5635868 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5658430 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5665854 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5665871 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5668481 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5668520 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5687112 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5689539 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-68948 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-69016 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-75825 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8850992 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8852354 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8854044 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8854071 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8866553 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8866858 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8932355 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8956140 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8956184 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8957265 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9755303 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9755306 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9766223 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-983150 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9907898 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9907919 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9908101 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9908178 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9908709 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9908721 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9912636 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9929352 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9929486 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9931314 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9934893 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9954728 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-NUL-212917 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-NUL-5610751 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005829 cytosol | TAS Reactome:R-NUL-9011324 | ACCEPT | Summary: cytosol is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005634 nucleus | IDA PMID:34711951 AKIRIN2 controls the nuclear import of proteasomes in verteb... | ACCEPT | Summary: nucleus is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0005737 cytoplasm | IDA PMID:34711951 AKIRIN2 controls the nuclear import of proteasomes in verteb... | KEEP AS NON CORE | Summary: cytoplasm is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0005839 proteasome core complex | IDA PMID:34711951 AKIRIN2 controls the nuclear import of proteasomes in verteb... | ACCEPT | Summary: proteasome core complex is the appropriate core-complex membership annotation for PSMB5. Reason: Structural studies and UniProt summaries place the protein in the 20S proteasome core; this complex-membership annotation is central to the gene product role. |
| GO:0006508 proteolysis | IDA PMID:17540904 Regulation of CD8+ T cell development by thymus-specific pro... | MODIFY | Summary: proteolysis is directionally related to the proteasome role but is less specific than the supported proteasome term. Reason: Replace the broad annotation with proteasomal protein catabolic process, which better captures the gene product role supported by proteasome literature and existing specific GOA annotations. Proposed replacements: proteasomal protein catabolic process |
| GO:0008233 peptidase activity | IDA PMID:17540904 Regulation of CD8+ T cell development by thymus-specific pro... | MODIFY | Summary: peptidase activity is directionally related to the proteasome role but is less specific than the supported proteasome term. Reason: Replace the broad annotation with threonine-type endopeptidase activity, which better captures the gene product role supported by proteasome literature and existing specific GOA annotations. Proposed replacements: threonine-type endopeptidase activity |
| GO:0000502 proteasome complex | IDA PMID:17323924 Mass spectrometric characterization of the affinity-purified... | KEEP AS NON CORE | Summary: proteasome complex is true complex membership but broader or more context-specific than the core 20S subunit identity. Reason: The core review should emphasize the specific 20S core subcomplex membership; broader proteasome or spermatoproteasome membership can be retained as non-core context. |
| GO:0005634 nucleus | HDA PMID:21630459 Proteomic characterization of the human sperm nucleus. | ACCEPT | Summary: nucleus is a core cellular context for the assembled proteasome containing PSMB5. Reason: The reviewed core function occurs in cytosolic and nuclear proteasome pools, supported by proteasome localization and nuclear-import evidence. |
| GO:0070062 extracellular exosome | HDA PMID:19056867 Large-scale proteomics and phosphoproteomics of urinary exos... | KEEP AS NON CORE | Summary: extracellular exosome is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-174058 | KEEP AS NON CORE | Summary: nucleoplasm is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-187574 | KEEP AS NON CORE | Summary: nucleoplasm is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-188191 | KEEP AS NON CORE | Summary: nucleoplasm is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-5635854 | KEEP AS NON CORE | Summary: nucleoplasm is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-68825 | KEEP AS NON CORE | Summary: nucleoplasm is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-69600 | KEEP AS NON CORE | Summary: nucleoplasm is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-8939801 | KEEP AS NON CORE | Summary: nucleoplasm is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-8952408 | KEEP AS NON CORE | Summary: nucleoplasm is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9762096 | KEEP AS NON CORE | Summary: nucleoplasm is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0005654 nucleoplasm | TAS Reactome:R-NUL-9604648 | KEEP AS NON CORE | Summary: nucleoplasm is a supported localization or active-location annotation for proteasomes but not the gene-specific core function. Reason: Proteasomes operate in cytosolic and nuclear compartments; localization annotations should be retained as context without replacing the core catalytic/structural role. |
| GO:0005839 proteasome core complex | ISS GO_REF:0000024 | ACCEPT | Summary: proteasome core complex is the appropriate core-complex membership annotation for PSMB5. Reason: Structural studies and UniProt summaries place the protein in the 20S proteasome core; this complex-membership annotation is central to the gene product role. |
| GO:0019774 proteasome core complex, beta-subunit complex | ISS GO_REF:0000024 | ACCEPT | Summary: proteasome core complex, beta-subunit complex is the appropriate core-complex membership annotation for PSMB5. Reason: Structural studies and UniProt summaries place the protein in the 20S proteasome core; this complex-membership annotation is central to the gene product role. |
| GO:0000502 proteasome complex | TAS PMID:8811196 Structure and functions of the 20S and 26S proteasomes. | KEEP AS NON CORE | Summary: proteasome complex is true complex membership but broader or more context-specific than the core 20S subunit identity. Reason: The core review should emphasize the specific 20S core subcomplex membership; broader proteasome or spermatoproteasome membership can be retained as non-core context. |
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Download this section (compressed HTML)Q: When should broad peptidase annotations on catalytic proteasome beta subunits be replaced by threonine-type endopeptidase activity in GOA cleanup?
Suggested experts: Schrader J, Chari A, Drag M
Experiment: Compare PSMB5 active-site or inhibitor-pocket mutants with PSMA1 alpha-ring interface mutants in matched cells or purified complexes, measuring chymotrypsin-like, caspase-like, and trypsin-like proteasome activities separately.
Hypothesis: PSMB5-specific perturbations should affect chymotrypsin-like proteasome activity more directly than non-catalytic alpha-ring subunit perturbations.
Type: comparative mutagenesis and proteasome activity profiling
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