PTPRN2 is a membrane protein of neuroendocrine secretory vesicles that traffics between secretory granules and the plasma membrane. It regulates vesicle accumulation and secretion and has a phosphatase-like intracellular domain. Conventional phosphoprotein activity is impaired, whereas phosphoinositide phosphatase activity and regulation of membrane PI(4,5)P2 have been reported in mammalian cells.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004725 protein tyrosine phosphatase activity | IEA GO_REF:0000120 | REMOVE | Summary: protein tyrosine phosphatase activity: PTPRN2 does not exhibit conventional phosphoprotein-substrate activity because of changes in its PTP domain; the human cell study explicitly distinguishes this from reported phosphoinositide phosphatase activity. The electronic protein-phosphatase activity assignment overgeneralizes the domain. Reason: PTPRN2 does not exhibit conventional phosphoprotein-substrate activity because of changes in its PTP domain; the human cell study explicitly distinguishes this from reported phosphoinositide phosphatase activity. The electronic protein-phosphatase activity assignment overgeneralizes the domain. Supporting Evidence: PMID:26620550 PTPRN2 belongs to the protein tyrosine phosphatase family, but does not exhibit activity against phosphoprotein substrates due to several critical amino acid variations in the PTP domain (Magistrelli etΒ al, 1996). |
| GO:0005001 transmembrane receptor protein tyrosine phosphatase activity | TAS PMID:8798755 Cloning and characterization of islet cell antigen-related p... | UNDECIDED | Summary: transmembrane receptor protein tyrosine phosphatase activity: The 1996 study reports recombinant phosphatase activity but its cached abstract does not identify the substrate; later work distinguishes lipid from phosphoprotein activity. The original assay and potential regulatory participation must be resolved before rejecting this experimental activity/process annotation. Reason: The 1996 study reports recombinant phosphatase activity but its cached abstract does not identify the substrate; later work distinguishes lipid from phosphoprotein activity. The original assay and potential regulatory participation must be resolved before rejecting this experimental activity/process annotation. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. |
| GO:0005886 plasma membrane | TAS PMID:8798755 Cloning and characterization of islet cell antigen-related p... | ACCEPT | Summary: plasma membrane: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Reason: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. file:human/PTPRN2/PTPRN2-uniprot.txt CC -!- FUNCTION: Plays a role in vesicle-mediated secretory processes. CC Required for normal accumulation of secretory vesicles in hippocampus, CC pituitary and pancreatic islets. Required for the accumulation of CC normal levels of insulin-containing vesicles and preventing their CC degradation. Plays a role in insulin secretion in response to glucose CC stimuli. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-6800426 | ACCEPT | Summary: plasma membrane: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Reason: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. file:human/PTPRN2/PTPRN2-uniprot.txt CC -!- FUNCTION: Plays a role in vesicle-mediated secretory processes. CC Required for normal accumulation of secretory vesicles in hippocampus, CC pituitary and pancreatic islets. Required for the accumulation of CC normal levels of insulin-containing vesicles and preventing their CC degradation. Plays a role in insulin secretion in response to glucose CC stimuli. |
| GO:0006470 protein dephosphorylation | TAS PMID:8798755 Cloning and characterization of islet cell antigen-related p... | UNDECIDED | Summary: protein dephosphorylation: The 1996 study reports recombinant phosphatase activity but its cached abstract does not identify the substrate; later work distinguishes lipid from phosphoprotein activity. The original assay and potential regulatory participation must be resolved before rejecting this experimental activity/process annotation. Reason: The 1996 study reports recombinant phosphatase activity but its cached abstract does not identify the substrate; later work distinguishes lipid from phosphoprotein activity. The original assay and potential regulatory participation must be resolved before rejecting this experimental activity/process annotation. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. |
| GO:0007269 neurotransmitter secretion | IEA GO_REF:0000107 | ACCEPT | Summary: neurotransmitter secretion: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Reason: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. file:human/PTPRN2/PTPRN2-uniprot.txt CC -!- FUNCTION: Plays a role in vesicle-mediated secretory processes. CC Required for normal accumulation of secretory vesicles in hippocampus, CC pituitary and pancreatic islets. Required for the accumulation of CC normal levels of insulin-containing vesicles and preventing their CC degradation. Plays a role in insulin secretion in response to glucose CC stimuli. |
| GO:0007269 neurotransmitter secretion | ISS GO_REF:0000024 | ACCEPT | Summary: neurotransmitter secretion: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Reason: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. file:human/PTPRN2/PTPRN2-uniprot.txt CC -!- FUNCTION: Plays a role in vesicle-mediated secretory processes. CC Required for normal accumulation of secretory vesicles in hippocampus, CC pituitary and pancreatic islets. Required for the accumulation of CC normal levels of insulin-containing vesicles and preventing their CC degradation. Plays a role in insulin secretion in response to glucose CC stimuli. |
| GO:0030141 secretory granule | IBA GO_REF:0000033 | ACCEPT | Summary: secretory granule: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Reason: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. file:human/PTPRN2/PTPRN2-uniprot.txt CC -!- FUNCTION: Plays a role in vesicle-mediated secretory processes. CC Required for normal accumulation of secretory vesicles in hippocampus, CC pituitary and pancreatic islets. Required for the accumulation of CC normal levels of insulin-containing vesicles and preventing their CC degradation. Plays a role in insulin secretion in response to glucose CC stimuli. |
| GO:0030141 secretory granule | IEA GO_REF:0000120 | ACCEPT | Summary: secretory granule: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Reason: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. file:human/PTPRN2/PTPRN2-uniprot.txt CC -!- FUNCTION: Plays a role in vesicle-mediated secretory processes. CC Required for normal accumulation of secretory vesicles in hippocampus, CC pituitary and pancreatic islets. Required for the accumulation of CC normal levels of insulin-containing vesicles and preventing their CC degradation. Plays a role in insulin secretion in response to glucose CC stimuli. |
| GO:0030658 transport vesicle membrane | IEA GO_REF:0000044 | ACCEPT | Summary: transport vesicle membrane: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Reason: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. file:human/PTPRN2/PTPRN2-uniprot.txt CC -!- FUNCTION: Plays a role in vesicle-mediated secretory processes. CC Required for normal accumulation of secretory vesicles in hippocampus, CC pituitary and pancreatic islets. Required for the accumulation of CC normal levels of insulin-containing vesicles and preventing their CC degradation. Plays a role in insulin secretion in response to glucose CC stimuli. |
| GO:0030667 secretory granule membrane | IEA GO_REF:0000107 | ACCEPT | Summary: secretory granule membrane: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Reason: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. file:human/PTPRN2/PTPRN2-uniprot.txt CC -!- FUNCTION: Plays a role in vesicle-mediated secretory processes. CC Required for normal accumulation of secretory vesicles in hippocampus, CC pituitary and pancreatic islets. Required for the accumulation of CC normal levels of insulin-containing vesicles and preventing their CC degradation. Plays a role in insulin secretion in response to glucose CC stimuli. |
| GO:0030667 secretory granule membrane | ISS GO_REF:0000024 | ACCEPT | Summary: secretory granule membrane: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Reason: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. file:human/PTPRN2/PTPRN2-uniprot.txt CC -!- FUNCTION: Plays a role in vesicle-mediated secretory processes. CC Required for normal accumulation of secretory vesicles in hippocampus, CC pituitary and pancreatic islets. Required for the accumulation of CC normal levels of insulin-containing vesicles and preventing their CC degradation. Plays a role in insulin secretion in response to glucose CC stimuli. |
| GO:0030672 synaptic vesicle membrane | IEA GO_REF:0000120 | ACCEPT | Summary: synaptic vesicle membrane: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Reason: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. file:human/PTPRN2/PTPRN2-uniprot.txt CC -!- FUNCTION: Plays a role in vesicle-mediated secretory processes. CC Required for normal accumulation of secretory vesicles in hippocampus, CC pituitary and pancreatic islets. Required for the accumulation of CC normal levels of insulin-containing vesicles and preventing their CC degradation. Plays a role in insulin secretion in response to glucose CC stimuli. |
| GO:0030672 synaptic vesicle membrane | ISS GO_REF:0000024 | ACCEPT | Summary: synaptic vesicle membrane: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Reason: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. file:human/PTPRN2/PTPRN2-uniprot.txt CC -!- FUNCTION: Plays a role in vesicle-mediated secretory processes. CC Required for normal accumulation of secretory vesicles in hippocampus, CC pituitary and pancreatic islets. Required for the accumulation of CC normal levels of insulin-containing vesicles and preventing their CC degradation. Plays a role in insulin secretion in response to glucose CC stimuli. |
| GO:0035773 insulin secretion involved in cellular response to glucose stimulus | IBA GO_REF:0000033 | ACCEPT | Summary: insulin secretion involved in cellular response to glucose stimulus: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Reason: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. file:human/PTPRN2/PTPRN2-uniprot.txt CC -!- FUNCTION: Plays a role in vesicle-mediated secretory processes. CC Required for normal accumulation of secretory vesicles in hippocampus, CC pituitary and pancreatic islets. Required for the accumulation of CC normal levels of insulin-containing vesicles and preventing their CC degradation. Plays a role in insulin secretion in response to glucose CC stimuli. |
| GO:0035773 insulin secretion involved in cellular response to glucose stimulus | IEA GO_REF:0000107 | ACCEPT | Summary: insulin secretion involved in cellular response to glucose stimulus: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Reason: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. file:human/PTPRN2/PTPRN2-uniprot.txt CC -!- FUNCTION: Plays a role in vesicle-mediated secretory processes. CC Required for normal accumulation of secretory vesicles in hippocampus, CC pituitary and pancreatic islets. Required for the accumulation of CC normal levels of insulin-containing vesicles and preventing their CC degradation. Plays a role in insulin secretion in response to glucose CC stimuli. |
| GO:0035773 insulin secretion involved in cellular response to glucose stimulus | ISS GO_REF:0000024 | ACCEPT | Summary: insulin secretion involved in cellular response to glucose stimulus: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Reason: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. file:human/PTPRN2/PTPRN2-uniprot.txt CC -!- FUNCTION: Plays a role in vesicle-mediated secretory processes. CC Required for normal accumulation of secretory vesicles in hippocampus, CC pituitary and pancreatic islets. Required for the accumulation of CC normal levels of insulin-containing vesicles and preventing their CC degradation. Plays a role in insulin secretion in response to glucose CC stimuli. |
| GO:0043235 signaling receptor complex | IDA PMID:23382219 Structural basis for endosomal trafficking of diverse transm... | UNDECIDED | Summary: signaling receptor complex: The available evidence examined for PTPRN2 does not resolve the exact claim of signaling receptor complex. The specific experiment or traced orthology/phylogenetic inference behind PMID:23382219 is needed; the annotation is not rejected from absence in a summary or from a different main focus of the paper. Reason: The available evidence examined for PTPRN2 does not resolve the exact claim of signaling receptor complex. The specific experiment or traced orthology/phylogenetic inference behind PMID:23382219 is needed; the annotation is not rejected from absence in a summary or from a different main focus of the paper. |
| GO:0045202 synapse | IBA GO_REF:0000033 | ACCEPT | Summary: synapse: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Reason: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. file:human/PTPRN2/PTPRN2-uniprot.txt CC -!- FUNCTION: Plays a role in vesicle-mediated secretory processes. CC Required for normal accumulation of secretory vesicles in hippocampus, CC pituitary and pancreatic islets. Required for the accumulation of CC normal levels of insulin-containing vesicles and preventing their CC degradation. Plays a role in insulin secretion in response to glucose CC stimuli. |
| GO:0051046 regulation of secretion | IBA GO_REF:0000033 | ACCEPT | Summary: regulation of secretion: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Reason: PTPRN2 is a secretory-vesicle membrane protein that traffics to the plasma membrane and regulates neuroendocrine secretion. Its vesicle role is supported independently of any protein tyrosine phosphatase claim. Supporting Evidence: PMID:26620550 As a transmembrane protein, PTPRN2 shuttles between secretory vesicles and the plasma membrane. file:human/PTPRN2/PTPRN2-uniprot.txt CC -!- FUNCTION: Plays a role in vesicle-mediated secretory processes. CC Required for normal accumulation of secretory vesicles in hippocampus, CC pituitary and pancreatic islets. Required for the accumulation of CC normal levels of insulin-containing vesicles and preventing their CC degradation. Plays a role in insulin secretion in response to glucose CC stimuli. |
| GO:0101003 ficolin-1-rich granule membrane | TAS Reactome:R-HSA-6800426 | UNDECIDED | Summary: ficolin-1-rich granule membrane: The available evidence examined for PTPRN2 does not resolve the exact claim of ficolin-1-rich granule membrane. The specific experiment or traced orthology/phylogenetic inference behind Reactome:R-HSA-6800426 is needed; the annotation is not rejected from absence in a summary or from a different main focus of the paper. Reason: The available evidence examined for PTPRN2 does not resolve the exact claim of ficolin-1-rich granule membrane. The specific experiment or traced orthology/phylogenetic inference behind Reactome:R-HSA-6800426 is needed; the annotation is not rejected from absence in a summary or from a different main focus of the paper. |
| GO:0052866 phosphatidylinositol phosphate phosphatase activity | IMP PMID:26620550 PTPRN2 and PLCΞ²1 promote metastatic breast cancer cell migra... | NEW | Summary: Human PTPRN2 has phosphoinositide phosphatase activity distinct from its impaired protein-phosphatase activity. Reason: The study connects PTPRN2-dependent PI(4,5)P2 levels to actin remodeling and tests catalytic-domain mutation. The broad phosphoinositide-phosphatase MF avoids inferring an unverified phosphate-position specificity. Supporting Evidence: PMID:26620550 Both PTPRN2 and PLCΞ²1 are enzymes with previously demonstrated activities for the substrate PI(4,5)P2 (Rebecchi & Pentyala, 2000; Caromile etΒ al, 2010). PMID:26620550 Mutation of the catalytic cysteine residue to the structurally similar serine residue abrogates PTPRN2's ability to dephosphorylate PI(4,5)P2 (Caromile etΒ al, 2010) PMID:26620550 We found that while wildβtype PTPRN2 overexpression was sufficient to increase invasion, migration, and metastatic lung colonization in mice, these effects were dependent on PTPRN2's catalytic activity as equivalent overexpression of PTPRN2C945A failed to enhance these phenotypes (FigΒ 2DβF). PMID:26620550 Both PTPRN2 and PLCΞ²1 also demonstrated localization to the plasma membrane in breast cancer cells, in addition to some cytoplasmic localization (FigΒ EV3A). |
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