RAB24

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0005776 autophagosome	IBA GO_REF:0000033	ACCEPT	Summary: autophagosome is a core RAB24 autophagy-associated location. Reason: RAB24 localizes to autophagic vacuoles/autophagic compartments and the autophagosome membrane context is directly relevant to its basal autophagic-compartment maturation/clearance role. Supporting Evidence: PMID:26325487 localization of RAB24 to autophagic vacuoles PMID:26325487 maturation and/or clearance of autophagic compartments under nutrient-rich conditions PMID:10660536 Posttranslational geranylgeranylation of Rab24 PMID:26325487 targeting of RAB24 to autophagic compartments
GO:0030139 endocytic vesicle	IBA GO_REF:0000033	KEEP AS NON CORE	Summary: Endocytic-vesicle activity is plausible Rab-effector context but not the core RAB24 PN function; falcon adds RAB24-specific late-endosomal (Rab7/RILP) degradation evidence. Reason: The IBA inference derives from a broad Rab/endocytic-vesicle family context. Beyond the rabenosyn-5 family-recognition data, the falcon deep research surfaces a more RAB24-specific late-endosomal role - RAB24 colocalizes with Rab7/LAMP1 and forms a complex with Rab7/RILP to support endosome-to-lysosome degradation (Amaya 2016). This strengthens an endocytic/late-endosomal context but remains a degradative late-compartment role rather than a defined early endocytic-vesicle trafficking step, so the core RAB24-specific process evidence is still autophagic-compartment maturation/clearance. The underlying Amaya 2016 paper is not in the cache and was not independently verified, so this is retained as non-core rather than promoted. Supporting Evidence: PMID:16034420 Rab GTPases interact with functionally diverse effectors PMID:16034420 rabenosyn-5 can achieve highly selective recognition file:human/RAB24/RAB24-deep-research-falcon.md RAB24 localizes to late endosomal compartments, where it colocalizes with markers such as Rab7 and LAMP1 file:human/RAB24/RAB24-deep-research-falcon.md Forms a complex with Rab7 and RILP on late endosomal membranes
GO:0000421 autophagosome membrane	IEA GO_REF:0000120	ACCEPT	Summary: autophagosome membrane is a core RAB24 autophagy-associated location, supported by immuno-EM on both limiting membranes. Reason: RAB24 localizes to autophagic vacuoles/autophagic compartments and the autophagosome membrane context is directly relevant to its basal autophagic-compartment maturation/clearance role. The falcon deep research adds that immuno-EM places RAB24 on both the inner and outer limiting membranes of autophagosomes/autophagic vacuoles, reinforcing this membrane localization. Supporting Evidence: PMID:26325487 localization of RAB24 to autophagic vacuoles PMID:26325487 maturation and/or clearance of autophagic compartments under nutrient-rich conditions PMID:10660536 Posttranslational geranylgeranylation of Rab24 PMID:26325487 targeting of RAB24 to autophagic compartments file:human/RAB24/RAB24-deep-research-falcon.md RAB24 localizes to both the inner and outer limiting membranes of autophagosomes and autophagic vacuoles
GO:0003924 GTPase activity	IEA GO_REF:0000002	ACCEPT	Summary: GTPase activity is consistent with RAB24 being an atypical Rab-family small GTPase. Reason: RAB24 is a Rab small GTPase; the evidence also cautions that it has unusually low GTP hydrolysis and is predominantly GTP-bound, but this does not negate the Rab GTPase/GTP-binding molecular-function annotation. Supporting Evidence: PMID:10660536 Rab24 exists predominantly in the GTP state PMID:10660536 The low GTPase activity is related to the presence of serine instead of glutamine
GO:0003925 G protein activity	IEA GO_REF:0000120	ACCEPT	Summary: G protein activity is consistent with RAB24 being an atypical Rab-family small GTPase. Reason: RAB24 is a Rab small GTPase; the evidence also cautions that it has unusually low GTP hydrolysis and is predominantly GTP-bound, but this does not negate the Rab GTPase/GTP-binding molecular-function annotation. Supporting Evidence: PMID:10660536 Rab24 exists predominantly in the GTP state PMID:10660536 The low GTPase activity is related to the presence of serine instead of glutamine
GO:0005525 GTP binding	IEA GO_REF:0000002	ACCEPT	Summary: GTP binding is consistent with RAB24 being an atypical Rab-family small GTPase. Reason: RAB24 is a Rab small GTPase; the evidence also cautions that it has unusually low GTP hydrolysis and is predominantly GTP-bound, but this does not negate the Rab GTPase/GTP-binding molecular-function annotation. Supporting Evidence: PMID:10660536 Rab24 exists predominantly in the GTP state PMID:10660536 The low GTPase activity is related to the presence of serine instead of glutamine
GO:0005819 spindle	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: spindle is transferred mouse oocyte/spindle biology and is not the central proteostasis function. Reason: UniProt and GOA support this as by-similarity mouse-oocyte/meiosis context. It may be biologically real in a reproductive setting, but it is secondary to RAB24 basal autophagic-compartment maturation/clearance for the human PN review. Supporting Evidence: file:human/RAB24/RAB24-uniprot.txt Involved in the modulation of meiotic apparatus assembly and meiotic progression during oocyte maturation file:human/RAB24/RAB24-uniprot.txt possibly through regulation of kinetochore-microtubule interaction
GO:0005829 cytosol	IEA GO_REF:0000044	KEEP AS NON CORE	Summary: cytosol is a broad RAB24 localization/context annotation. Reason: RAB24 can be cytosolic and membrane-associated, with perinuclear/autophagic-compartment localization reported by similarity, but these broad terms should not be treated as the core location when autophagosome/autophagosome membrane is available. Supporting Evidence: PMID:10660536 Posttranslational geranylgeranylation of Rab24 PMID:26325487 targeting of RAB24 to autophagic compartments file:human/RAB24/RAB24-uniprot.txt Only about 20% is recovered in the particulate fraction
GO:0016020 membrane	IEA GO_REF:0000044	KEEP AS NON CORE	Summary: membrane is a broad RAB24 localization/context annotation. Reason: RAB24 can be cytosolic and membrane-associated, with perinuclear/autophagic-compartment localization reported by similarity, but these broad terms should not be treated as the core location when autophagosome/autophagosome membrane is available. Supporting Evidence: PMID:10660536 Posttranslational geranylgeranylation of Rab24 PMID:26325487 targeting of RAB24 to autophagic compartments file:human/RAB24/RAB24-uniprot.txt Only about 20% is recovered in the particulate fraction
GO:0048471 perinuclear region of cytoplasm	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: perinuclear region of cytoplasm is a broad RAB24 localization/context annotation. Reason: RAB24 can be cytosolic and membrane-associated, with perinuclear/autophagic-compartment localization reported by similarity, but these broad terms should not be treated as the core location when autophagosome/autophagosome membrane is available. Supporting Evidence: PMID:10660536 Posttranslational geranylgeranylation of Rab24 PMID:26325487 targeting of RAB24 to autophagic compartments file:human/RAB24/RAB24-uniprot.txt Only about 20% is recovered in the particulate fraction
GO:0098588 bounding membrane of organelle	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: bounding membrane of organelle is a broad RAB24 localization/context annotation. Reason: RAB24 can be cytosolic and membrane-associated, with perinuclear/autophagic-compartment localization reported by similarity, but these broad terms should not be treated as the core location when autophagosome/autophagosome membrane is available. Supporting Evidence: PMID:10660536 Posttranslational geranylgeranylation of Rab24 PMID:26325487 targeting of RAB24 to autophagic compartments file:human/RAB24/RAB24-uniprot.txt Only about 20% is recovered in the particulate fraction
GO:0005515 protein binding	IPI PMID:20562859 Network organization of the human autophagy system.	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not describe the actual RAB24 molecular function. Reason: The interaction record may identify a binding partner, but protein binding is uninformative as a GO molecular function. RAB24 should be represented by Rab-family GTPase/GTP-binding activity and, where evidence is specific, autophagic-compartment localization/process terms.
GO:0005515 protein binding	IPI PMID:32296183 A reference map of the human binary protein interactome.	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not describe the actual RAB24 molecular function. Reason: The interaction record may identify a binding partner, but protein binding is uninformative as a GO molecular function. RAB24 should be represented by Rab-family GTPase/GTP-binding activity and, where evidence is specific, autophagic-compartment localization/process terms.
GO:0005515 protein binding	IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not describe the actual RAB24 molecular function. Reason: The interaction record may identify a binding partner, but protein binding is uninformative as a GO molecular function. RAB24 should be represented by Rab-family GTPase/GTP-binding activity and, where evidence is specific, autophagic-compartment localization/process terms.
GO:0005515 protein binding	IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not describe the actual RAB24 molecular function. Reason: The interaction record may identify a binding partner, but protein binding is uninformative as a GO molecular function. RAB24 should be represented by Rab-family GTPase/GTP-binding activity and, where evidence is specific, autophagic-compartment localization/process terms.
GO:0001556 oocyte maturation	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: oocyte maturation is transferred mouse oocyte/spindle biology and is not the central proteostasis function. Reason: UniProt and GOA support this as by-similarity mouse-oocyte/meiosis context. It may be biologically real in a reproductive setting, but it is secondary to RAB24 basal autophagic-compartment maturation/clearance for the human PN review. Supporting Evidence: file:human/RAB24/RAB24-uniprot.txt Involved in the modulation of meiotic apparatus assembly and meiotic progression during oocyte maturation file:human/RAB24/RAB24-uniprot.txt possibly through regulation of kinetochore-microtubule interaction
GO:0005737 cytoplasm	IEA GO_REF:0000120	KEEP AS NON CORE	Summary: cytoplasm is a broad RAB24 localization/context annotation. Reason: RAB24 can be cytosolic and membrane-associated, with perinuclear/autophagic-compartment localization reported by similarity, but these broad terms should not be treated as the core location when autophagosome/autophagosome membrane is available. Supporting Evidence: PMID:10660536 Posttranslational geranylgeranylation of Rab24 PMID:26325487 targeting of RAB24 to autophagic compartments file:human/RAB24/RAB24-uniprot.txt Only about 20% is recovered in the particulate fraction
GO:0005776 autophagosome	IEA GO_REF:0000107	ACCEPT	Summary: autophagosome is a core RAB24 autophagy-associated location. Reason: RAB24 localizes to autophagic vacuoles/autophagic compartments and the autophagosome membrane context is directly relevant to its basal autophagic-compartment maturation/clearance role. Supporting Evidence: PMID:26325487 localization of RAB24 to autophagic vacuoles PMID:26325487 maturation and/or clearance of autophagic compartments under nutrient-rich conditions PMID:10660536 Posttranslational geranylgeranylation of Rab24 PMID:26325487 targeting of RAB24 to autophagic compartments
GO:0006914 autophagy	IEA GO_REF:0000107	MODIFY	Summary: Generic autophagy is too broad for the RAB24-specific evidence. Reason: The RAB24-specific study places the function at maturation and/or clearance of late autophagic compartments under basal nutrient-rich conditions, while formation and short starvation-induced autophagy are not affected. Replace the broad autophagy term with autophagosome maturation. Proposed replacements: autophagosome maturation Supporting Evidence: PMID:26325487 localization of RAB24 to autophagic vacuoles PMID:26325487 maturation and/or clearance of autophagic compartments under nutrient-rich conditions PMID:26325487 Formation of autophagosomes is shown to be unaffected by RAB24-silencing with siRNA
GO:0008608 attachment of spindle microtubules to kinetochore	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: attachment of spindle microtubules to kinetochore is transferred mouse oocyte/spindle biology and is not the central proteostasis function. Reason: UniProt and GOA support this as by-similarity mouse-oocyte/meiosis context. It may be biologically real in a reproductive setting, but it is secondary to RAB24 basal autophagic-compartment maturation/clearance for the human PN review. Supporting Evidence: file:human/RAB24/RAB24-uniprot.txt Involved in the modulation of meiotic apparatus assembly and meiotic progression during oocyte maturation file:human/RAB24/RAB24-uniprot.txt possibly through regulation of kinetochore-microtubule interaction
GO:0140013 meiotic nuclear division	IEA GO_REF:0000107	KEEP AS NON CORE	Summary: meiotic nuclear division is transferred mouse oocyte/spindle biology and is not the central proteostasis function. Reason: UniProt and GOA support this as by-similarity mouse-oocyte/meiosis context. It may be biologically real in a reproductive setting, but it is secondary to RAB24 basal autophagic-compartment maturation/clearance for the human PN review. Supporting Evidence: file:human/RAB24/RAB24-uniprot.txt Involved in the modulation of meiotic apparatus assembly and meiotic progression during oocyte maturation file:human/RAB24/RAB24-uniprot.txt possibly through regulation of kinetochore-microtubule interaction
GO:0005829 cytosol	IDA GO_REF:0000052	KEEP AS NON CORE	Summary: cytosol is a broad RAB24 localization/context annotation. Reason: RAB24 can be cytosolic and membrane-associated, with perinuclear/autophagic-compartment localization reported by similarity, but these broad terms should not be treated as the core location when autophagosome/autophagosome membrane is available. Supporting Evidence: PMID:10660536 Posttranslational geranylgeranylation of Rab24 PMID:26325487 targeting of RAB24 to autophagic compartments file:human/RAB24/RAB24-uniprot.txt Only about 20% is recovered in the particulate fraction
GO:0003925 G protein activity	ISS GO_REF:0000024	ACCEPT	Summary: G protein activity is consistent with RAB24 being an atypical Rab-family small GTPase. Reason: RAB24 is a Rab small GTPase; the evidence also cautions that it has unusually low GTP hydrolysis and is predominantly GTP-bound, but this does not negate the Rab GTPase/GTP-binding molecular-function annotation. Supporting Evidence: PMID:10660536 Rab24 exists predominantly in the GTP state PMID:10660536 The low GTPase activity is related to the presence of serine instead of glutamine
GO:0003924 GTPase activity	ISS GO_REF:0000024	ACCEPT	Summary: GTPase activity is consistent with RAB24 being an atypical Rab-family small GTPase. Reason: RAB24 is a Rab small GTPase; the evidence also cautions that it has unusually low GTP hydrolysis and is predominantly GTP-bound, but this does not negate the Rab GTPase/GTP-binding molecular-function annotation. Supporting Evidence: PMID:10660536 Rab24 exists predominantly in the GTP state PMID:10660536 The low GTPase activity is related to the presence of serine instead of glutamine
GO:0016020 membrane	EXP PMID:10660536 Rab24 is an atypical member of the Rab GTPase family. Defici...	KEEP AS NON CORE	Summary: membrane is a broad RAB24 localization/context annotation. Reason: RAB24 can be cytosolic and membrane-associated, with perinuclear/autophagic-compartment localization reported by similarity, but these broad terms should not be treated as the core location when autophagosome/autophagosome membrane is available. Supporting Evidence: PMID:10660536 Posttranslational geranylgeranylation of Rab24 PMID:26325487 targeting of RAB24 to autophagic compartments file:human/RAB24/RAB24-uniprot.txt Only about 20% is recovered in the particulate fraction
GO:0001556 oocyte maturation	ISS GO_REF:0000024	KEEP AS NON CORE	Summary: oocyte maturation is transferred mouse oocyte/spindle biology and is not the central proteostasis function. Reason: UniProt and GOA support this as by-similarity mouse-oocyte/meiosis context. It may be biologically real in a reproductive setting, but it is secondary to RAB24 basal autophagic-compartment maturation/clearance for the human PN review. Supporting Evidence: file:human/RAB24/RAB24-uniprot.txt Involved in the modulation of meiotic apparatus assembly and meiotic progression during oocyte maturation file:human/RAB24/RAB24-uniprot.txt possibly through regulation of kinetochore-microtubule interaction
GO:0008608 attachment of spindle microtubules to kinetochore	ISS GO_REF:0000024	KEEP AS NON CORE	Summary: attachment of spindle microtubules to kinetochore is transferred mouse oocyte/spindle biology and is not the central proteostasis function. Reason: UniProt and GOA support this as by-similarity mouse-oocyte/meiosis context. It may be biologically real in a reproductive setting, but it is secondary to RAB24 basal autophagic-compartment maturation/clearance for the human PN review. Supporting Evidence: file:human/RAB24/RAB24-uniprot.txt Involved in the modulation of meiotic apparatus assembly and meiotic progression during oocyte maturation file:human/RAB24/RAB24-uniprot.txt possibly through regulation of kinetochore-microtubule interaction
GO:0140013 meiotic nuclear division	ISS GO_REF:0000024	KEEP AS NON CORE	Summary: meiotic nuclear division is transferred mouse oocyte/spindle biology and is not the central proteostasis function. Reason: UniProt and GOA support this as by-similarity mouse-oocyte/meiosis context. It may be biologically real in a reproductive setting, but it is secondary to RAB24 basal autophagic-compartment maturation/clearance for the human PN review. Supporting Evidence: file:human/RAB24/RAB24-uniprot.txt Involved in the modulation of meiotic apparatus assembly and meiotic progression during oocyte maturation file:human/RAB24/RAB24-uniprot.txt possibly through regulation of kinetochore-microtubule interaction
GO:0000421 autophagosome membrane	ISS GO_REF:0000024	ACCEPT	Summary: autophagosome membrane is a core RAB24 autophagy-associated location. Reason: RAB24 localizes to autophagic vacuoles/autophagic compartments and the autophagosome membrane context is directly relevant to its basal autophagic-compartment maturation/clearance role. Supporting Evidence: PMID:26325487 localization of RAB24 to autophagic vacuoles PMID:26325487 maturation and/or clearance of autophagic compartments under nutrient-rich conditions PMID:10660536 Posttranslational geranylgeranylation of Rab24 PMID:26325487 targeting of RAB24 to autophagic compartments
GO:0006914 autophagy	ISS GO_REF:0000024	MODIFY	Summary: Generic autophagy is too broad for the RAB24-specific evidence. Reason: The RAB24-specific study places the function at maturation and/or clearance of late autophagic compartments under basal nutrient-rich conditions, while formation and short starvation-induced autophagy are not affected. Replace the broad autophagy term with autophagosome maturation. Proposed replacements: autophagosome maturation Supporting Evidence: PMID:26325487 localization of RAB24 to autophagic vacuoles PMID:26325487 maturation and/or clearance of autophagic compartments under nutrient-rich conditions PMID:26325487 Formation of autophagosomes is shown to be unaffected by RAB24-silencing with siRNA
GO:0048471 perinuclear region of cytoplasm	ISS GO_REF:0000024	KEEP AS NON CORE	Summary: perinuclear region of cytoplasm is a broad RAB24 localization/context annotation. Reason: RAB24 can be cytosolic and membrane-associated, with perinuclear/autophagic-compartment localization reported by similarity, but these broad terms should not be treated as the core location when autophagosome/autophagosome membrane is available. Supporting Evidence: PMID:10660536 Posttranslational geranylgeranylation of Rab24 PMID:26325487 targeting of RAB24 to autophagic compartments file:human/RAB24/RAB24-uniprot.txt Only about 20% is recovered in the particulate fraction
GO:0005739 mitochondrion	HTP PMID:34800366 Quantitative high-confidence human mitochondrial proteome an...	MARK AS OVER ANNOTATED	Summary: Mitochondrion is not supported as a core RAB24 location. Reason: This high-throughput proteome-derived row does not match the RAB24-specific localization/function evidence, which centers on cytosol, membrane association, and autophagic compartments. Supporting Evidence: file:human/RAB24/RAB24-uniprot.txt Cytoplasm, cytosol PMID:26325487 localization of RAB24 to autophagic vacuoles
GO:0005886 plasma membrane	TAS Reactome:R-HSA-6798743	MARK AS OVER ANNOTATED	Summary: plasma membrane is Reactome degranulation context, not a specific RAB24 core location. Reason: The reviewed RAB24 evidence supports autophagic-compartment/autophagosome membrane localization. Reactome secretory-granule/plasma-membrane context should not be propagated as a core RAB24 cellular-component annotation. Supporting Evidence: PMID:26325487 localization of RAB24 to autophagic vacuoles PMID:26325487 maturation and/or clearance of autophagic compartments under nutrient-rich conditions
GO:0030667 secretory granule membrane	TAS Reactome:R-HSA-6798743	MARK AS OVER ANNOTATED	Summary: secretory granule membrane is Reactome degranulation context, not a specific RAB24 core location. Reason: The reviewed RAB24 evidence supports autophagic-compartment/autophagosome membrane localization. Reactome secretory-granule/plasma-membrane context should not be propagated as a core RAB24 cellular-component annotation. Supporting Evidence: PMID:26325487 localization of RAB24 to autophagic vacuoles PMID:26325487 maturation and/or clearance of autophagic compartments under nutrient-rich conditions
GO:0005515 protein binding	IPI PMID:16034420 Structural basis of family-wide Rab GTPase recognition by ra...	MARK AS OVER ANNOTATED	Summary: Generic protein binding does not describe the actual RAB24 molecular function. Reason: The interaction record may identify a binding partner, but protein binding is uninformative as a GO molecular function. RAB24 should be represented by Rab-family GTPase/GTP-binding activity and, where evidence is specific, autophagic-compartment localization/process terms. Supporting Evidence: PMID:16034420 Rab GTPases interact with functionally diverse effectors PMID:16034420 rabenosyn-5 can achieve highly selective recognition

Gene Description

Existing Annotations Review

Core Functions

RAB24 is an atypical Rab-family small GTPase whose GTP-bound state supports targeting to autophagic compartments and late basal autophagic-compartment maturation/clearance.

RAB24 nucleotide binding is required for its autophagic-compartment targeting, but the protein is atypical because it has weak GTP hydrolysis and remains predominantly GTP-bound.

References

Suggested Questions for Experts

Suggested Experiments

Deep Research

Falcon

📚 Additional Documentation

Notes

Pn Notes

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