| Category | RAB24 characteristic | Evidence / notes | Citation |
|---|---|---|---|
| Basic protein information | Ras-related protein Rab-24; small GTPase of the Rab family within the Ras superfamily | Reviews describe RAB24 as an atypical Rab-family small GTPase involved in membrane trafficking; Rab proteins generally act as molecular switches cycling between GTP- and GDP-bound states | (pqac-00000002, pqac-00000008, pqac-00000013) |
| Basic protein information | Approx. 24 kDa molecular mass | Multiple reviews explicitly refer to “Rab24 GTPase (24 kDa)” | (pqac-00000006, pqac-00000007) |
| Basic protein information | Enzyme class: small GTPase / guanosine triphosphate hydrolase activity | Rab proteins hydrolyze GTP and are regulated by GEFs/GAPs/GDIs; this applies to RAB24 as a Rab-family member | (pqac-00000002, pqac-00000010, pqac-00000012) |
| Basic protein information | Atypical Rab with unusually low intrinsic GTPase activity and predominant GTP-bound state reported in earlier work summarized by later reviews | Review of RAB24 biology notes distinctive biochemical features compared with typical Rabs | (pqac-00000008, pqac-00000010) |
| Subcellular localization | Endoplasmic reticulum and ER/cis-Golgi region | RAB24 was first described at the ER, Golgi, and late endosomes; later reviews summarize localization at ER/cis-Golgi | (pqac-00000003, pqac-00000006, pqac-00000009) |
| Subcellular localization | Late endosomes / lysosome-directed late compartments | Primary work found Rab24 colocalization with Rab7- and LAMP1-positive vesicles and participation in endosome-lysosome degradation | (pqac-00000007) |
| Subcellular localization | Autophagic vacuoles, including LC3-positive autophagic compartments | Under starvation and basal conditions, RAB24 relocalizes to autophagic vacuoles and colocalizes with LC3 | (pqac-00000006, pqac-00000008, pqac-00000009) |
| Subcellular localization | Inner and outer limiting membranes of autophagic vacuoles | Immuno-EM showed RAB24 on both inner and outer autophagosomal/autophagic vacuole membranes | (pqac-00000009) |
| Primary molecular functions | Regulator of intracellular membrane trafficking | Rab-family reviews define Rabs as organizers of vesicle biogenesis, transport, tethering, and fusion; RAB24 is one of these membrane-trafficking regulators | (pqac-00000002, pqac-00000013) |
| Primary molecular functions | Facilitates clearance/maturation of late autophagic compartments, especially under basal nutrient-rich conditions | RAB24 knockdown increased late autophagic compartments without increasing autophagosome formation, supporting a late-stage clearance role | (pqac-00000009, pqac-00000008) |
| Primary molecular functions | Promotes endosomal degradative trafficking | Rab24 forms a complex with Rab7/RILP and is required for efficient DQ-BSA degradation and Rab7 membrane recruitment | (pqac-00000007) |
| Key biological processes / pathways | Basal macroautophagy | Reviews and primary work support a specific role in basal autophagy rather than early autophagosome biogenesis | (pqac-00000008, pqac-00000009) |
| Key biological processes / pathways | Autophagosome maturation / autophagic vacuole clearance | RAB24 is linked to maturation/clearance of degradative autophagic compartments and accumulation of late autophagic vacuoles when absent | (pqac-00000008, pqac-00000009) |
| Key biological processes / pathways | Endosome-to-lysosome degradative pathway | Rab24 functions with Rab7/RILP on late endosomal membranes in the last steps of endosomal degradation | (pqac-00000007) |
| Key biological processes / pathways | Xenophagy / infection-related autophagy contexts | RAB24 is listed among Rab proteins participating in infection-related autophagy responses, including GAS-targeting autophagy | (pqac-00000005, pqac-00000006) |
| Molecular interactions | Rab7 | Direct interaction reported; Rab24 supports Rab7 association with vesicular membranes in late endosomal degradation | (pqac-00000007) |
| Molecular interactions | RILP (Rab-interacting lysosomal protein) | Rab24 interacts with the Rab7/RILP complex, linking it to dynein-associated late endosomal trafficking | (pqac-00000007) |
| Molecular interactions | LC3 / MAP1LC3-positive autophagic membranes | Colocalization with LC3-positive puncta and autophagic vacuoles is repeatedly observed | (pqac-00000006, pqac-00000009, pqac-00000011) |
| Molecular interactions | Late endosomal/lysosomal markers such as LAMP1 | Colocalization with LAMP1-positive compartments supports a late endosomal/lysosomal role | (pqac-00000007) |
| Post-translational modifications | Prenylation at the C-terminus required for membrane targeting | Rab-family reviews explain prenylation-dependent membrane association; RAB24-specific work showed prenylation is necessary for localization to autophagic vacuoles | (pqac-00000001, pqac-00000008, pqac-00000009) |
| Post-translational modifications | Tyrosine phosphorylation at Y17 and/or Y172 | Review of Rab phosphorylation notes Rab24 phosphorylation at Y17/Y172; Y17 phosphorylation may affect intrinsic GTPase activity | (pqac-00000010) |
| Disease associations | Neurodegeneration / ataxia | Canine hereditary ataxia-causing RAB24 variants are associated with Purkinje neuron degeneration, autolysosome accumulation, and protein aggregates; authors suggest human relevance | (pqac-00000003, pqac-00000008) |
| Disease associations | Hepatocellular carcinoma | Reviews summarize evidence that elevated RAB24 promotes motility, invasion, cell-cycle progression, EMT, and poor prognosis in HCC contexts | (pqac-00000003, pqac-00000012) |
| Disease associations | Pancreatic adenocarcinoma prognosis | RAB24 has been reported as an independent low-risk/favorable factor in pancreatic adenocarcinoma according to summarized literature | (pqac-00000003) |
| Disease associations | Prostate cancer prognosis | High RAB24 expression has been reported as an unfavorable prognostic marker in prostate cancer | (pqac-00000003) |
| Disease associations | Fatty liver / NAFLD | Liver RAB24 levels positively correlate with body fat and are elevated in obese patients with NAFLD, according to summarized literature | (pqac-00000003) |
| Disease associations | Broad cancer relevance with context-dependent expression | Tissue survey/review data indicate higher RAB24 in some cancers (e.g., breast, skin, pediatric neuronal tumors) and lower in others, implying context-dependent roles | (pqac-00000003, pqac-00000004, pqac-00000013) |


*Table: This table summarizes the main experimentally supported and review-supported properties of human RAB24, including its localization, trafficking functions, regulatory features, and disease links. It is useful as a compact evidence map for functional annotation of UniProt Q969Q5.*