| Category | Key finding | Evidence type | Source (with URL/date if available) | Citation ID(s) |
|---|---|---|---|---|
| Verified identifiers | Human **RASA4B** is linked to **UniProt C9J798** and, via Open Targets evidence, **Ensembl ENSG00000170667**; Open Targets approved name: **RAS p21 protein activator 4B** | Curated target/disease platform; peer-reviewed proteomics review table | Open Targets target association output for RASA4B (accessed via tool; no publication date in output); O'Donoghue & Smolenski, *Biosci Rep* 2024-05, https://doi.org/10.1042/BSR20231420 | (pqac-00000000, pqac-00000004) |
| Distinction from RASA4 | The 2024 platelet GAP review lists **RASA4B (C9J798)** and **RASA4 (O43374)** as separate entries, supporting that retrieved evidence treats them as distinct identifiers rather than the same protein entry | Peer-reviewed review table | O'Donoghue & Smolenski, *Biosci Rep* 2024-05, https://doi.org/10.1042/BSR20231420 | (pqac-00000002, pqac-00000004, pqac-00000011) |
| Literature-reported status | An evolutionary genomics analysis states that **RASA4 and RASA4B both map to chromosome 7** and that **RASA4B appears to be a truncated version of RASA4 annotated as a pseudogene**; this creates an annotation conflict/ambiguity relative to UniProt C9J798 being listed as a protein entry | Peer-reviewed evolutionary/genomics analysis | Díez et al., *Nucleic Acids Res* 2011-03, https://doi.org/10.1093/nar/gkr154 | (pqac-00000007) |
| Domain architecture (database annotation) | User-provided UniProt/InterPro annotation for **C9J798/RASA4B** includes **PH domain, C2 domain, and Ras GTPase-activating (RasGAP) domain/superfamily**. In retrieved literature, these domains were **not directly confirmed experimentally for RASA4B** itself | Database/domain annotation supplied in prompt; cautious synthesis | UniProt C9J798 / InterPro terms provided by user: C2_dom (IPR000008), C2_domain_sf (IPR035892), PH-like_dom_sf (IPR011993), PH_domain (IPR001849), Ras_GTPase (IPR039360) | (pqac-00000004) |
| Family-level functional inference only | Retrieved RasGAP/GAP1-family reviews support that related proteins in this family use membrane-targeting/regulatory domains (C2/PH) to control Ras/Rap GAP activity, but **no direct RASA4B enzymology or localization study was retrieved** | Review-based family inference, not direct RASA4B evidence | King et al., *Sci Signal* 2013-02, https://doi.org/10.1126/scisignal.2003669; Molina-Ortiz et al., *PLoS Genet* 2018-01, https://doi.org/10.1371/journal.pgen.1007195 | (pqac-00000008, pqac-00000009) |
| Experimental/observational evidence in retrieved sources | In a 2024 review summarizing human platelet proteomics, **RASA4B is listed as detected/present in the platelet GAP landscape**, but the **protein copy number per platelet is not reported** (shown as “-”); nearby rows show copy numbers for RASA1 and RASA3, underscoring that the missing value is specific to available quantitation | Review table summarizing platelet proteomics datasets | O'Donoghue & Smolenski, *Biosci Rep* 2024-05, https://doi.org/10.1042/BSR20231420 | (pqac-00000004, pqac-00000011) |
| Platelet comparison context | In the same table, **RASA1 = 2921 copies/platelet** and **RASA3 = 8293 copies/platelet**, whereas **RASA4B** and **RASA4** lack reported copy numbers, indicating weaker or unquantified support for abundance despite inclusion in the GAP inventory | Quantitative review table | O'Donoghue & Smolenski, *Biosci Rep* 2024-05, https://doi.org/10.1042/BSR20231420 | (pqac-00000004, pqac-00000011) |
| Open Targets disease association | **Neurodegenerative disease** association score **0.3368**; evidence count **5**; linked literature in output includes **PMID: 34031600** and **PMID: 16041389** | Integrated target–disease evidence platform | Open Targets evidence output for RASA4B (tool context) | (pqac-00000000) |
| Open Targets disease association | **Circadian rhythm** association score **0.0798**; evidence count **5**; linked literature includes **PMID: 34031600**, **16041389** | Integrated target–disease evidence platform | Open Targets evidence output for RASA4B (tool context) | (pqac-00000000) |
| Open Targets disease association | **Immunodeficiency 35** association score **0.0626**; evidence count **5**; linked literature includes **PMID: 34031600**, **16041389** | Integrated target–disease evidence platform | Open Targets evidence output for RASA4B (tool context) | (pqac-00000000) |
| Open Targets disease association | **Immunodeficiency due to a late component of complements deficiency** association score **0.0722**; evidence count **5**; linked literature includes **PMID: 34031600**, **16041389** | Integrated target–disease evidence platform | Open Targets evidence output for RASA4B (tool context) | (pqac-00000000) |
| Open Targets disease association | **Autosomal recessive hyper-IgE syndrome** association score **0.0782**; evidence count **5**; linked literature includes **PMID: 34031600**, **16041389** | Integrated target–disease evidence platform | Open Targets evidence output for RASA4B (tool context) | (pqac-00000000) |
| Overall evidence appraisal | Direct experimental literature specifically on **human RASA4B/C9J798** is **very limited in retrieved sources**. The strongest direct evidence is identifier-level distinction from RASA4 and observational inclusion in platelet proteomics summaries; mechanistic function remains largely **database-annotated or inferred from related RasGAP family members** | Evidence synthesis | Synthesized from retrieved evidence above | (pqac-00000000, pqac-00000004, pqac-00000007, pqac-00000008, pqac-00000009, pqac-00000011) |


*Table: This table consolidates the strongest retrieved evidence specific to human RASA4B, including verified identifiers, distinction from RASA4, literature ambiguity about truncation/pseudogene status, database-annotated domains, platelet proteomics observations, and Open Targets disease associations. It is useful for separating direct evidence from cautious inference when annotating this poorly characterized target.*