RBPJ (recombination signal binding protein for immunoglobulin kappa J region; also CBF1/CSL, the human Suppressor of Hairless/LAG-1 ortholog) is the sequence-specific DNA-binding transcription factor of the canonical Notch pathway. Its N-terminal (NTD) and beta-trefoil (BTD) domains bind the core motif C/TGTGGGAA; the BTD and C-terminal domain (CTD) form the surface that captures the RAM and ankyrin regions of the Notch intracellular domain (NICD). In the absence of NICD, RBPJ acts as a repressor by recruiting corepressors such as SPEN/SHARP, NCOR2/SMRT, CIR, L3MBTL3-KDM1A and CtBP/CtIP with histone deacetylase/demethylase activities. NICD binding displaces corepressors and creates a composite groove that recruits Mastermind-like coactivators (MAML1-3), forming the CSL-NICD-MAML ternary complex that activates target genes such as HES1, HEY1 and NRARP; on paired, head-to-head sites the complexes dimerize cooperatively. RBPJ is exploited by Epstein-Barr virus EBNA2/EBNA3 proteins. In development, RBPJ-dependent Notch transcription controls cardiac, vascular, hematopoietic and many other cell-fate decisions; RBPJ variants cause Adams-Oliver syndrome.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000122 negative regulation of transcription by RNA polymerase II | IEA GO_REF:0000120 | ACCEPT | Summary: RBPJ represses Notch targets in the absence of NICD by recruiting corepressors. Reason: Core repressor activity of CSL proteins. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Acts as a transcriptional repressor when it is not associated with Notch proteins. PMID:10713164 CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex. |
| GO:0000122 negative regulation of transcription by RNA polymerase II | IMP PMID:18663143 Slug is a direct Notch target required for initiation of car... | ACCEPT | Summary: RBPJ represses Notch targets in the absence of NICD by recruiting corepressors. Reason: Core repressor activity of CSL proteins. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Acts as a transcriptional repressor when it is not associated with Notch proteins. PMID:10713164 CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex. |
| GO:0000122 negative regulation of transcription by RNA polymerase II | ISS GO_REF:0000024 | ACCEPT | Summary: RBPJ represses Notch targets in the absence of NICD by recruiting corepressors. Reason: Core repressor activity of CSL proteins. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Acts as a transcriptional repressor when it is not associated with Notch proteins. PMID:10713164 CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex. |
| GO:0000785 chromatin | ISA GO_REF:0000113 | ACCEPT | Summary: Chromatin (TFClass). Reason: RBPJ acts at target enhancers on chromatin. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. |
| GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding | IBA GO_REF:0000033 | ACCEPT | Summary: RBPJ binds its cognate cis-regulatory motif at Notch target promoters/enhancers. Reason: Sequence-specific DNA binding at RNA polymerase II cis-regulatory regions is the core MF of CSL proteins; conserved from flies to yeast-like CSL proteins (PTN000071433). Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. PMID:16530044 We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). |
| GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding | IDA PMID:16691198 A metabolic enzyme of the short-chain dehydrogenase/reductas... | ACCEPT | Summary: RBPJ binds its cognate cis-regulatory motif at Notch target promoters/enhancers. Reason: Sequence-specific DNA binding at RNA polymerase II cis-regulatory regions is the core MF of CSL proteins; conserved from flies to yeast-like CSL proteins (PTN000071433). Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. PMID:16530044 We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). |
| GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding | IEA GO_REF:0000120 | ACCEPT | Summary: RBPJ binds its cognate cis-regulatory motif at Notch target promoters/enhancers. Reason: Sequence-specific DNA binding at RNA polymerase II cis-regulatory regions is the core MF of CSL proteins; conserved from flies to yeast-like CSL proteins (PTN000071433). Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. PMID:16530044 We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). |
| GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific | IBA GO_REF:0000033 | ACCEPT | Summary: RBPJ is a DNA-binding transcription factor that represses or activates RNA polymerase II targets. Reason: Core MF of CSL; supported by phylogeny (PTN000071433), TFClass and experiments. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Acts as a transcriptional repressor when it is not associated with Notch proteins. file:human/RBPJ/RBPJ-uniprot.txt When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. |
| GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific | ISA GO_REF:0000113 | ACCEPT | Summary: RBPJ is a DNA-binding transcription factor that represses or activates RNA polymerase II targets. Reason: Core MF of CSL; supported by phylogeny (PTN000071433), TFClass and experiments. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Acts as a transcriptional repressor when it is not associated with Notch proteins. file:human/RBPJ/RBPJ-uniprot.txt When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. |
| GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific | IDA PMID:18663143 Slug is a direct Notch target required for initiation of car... | ACCEPT | Summary: In complex with NICD/MAML, RBPJ activates targets such as Slug/SNAI2, HEY genes and COX4I2. Reason: Activator activity is a core state of RBPJ; CSL knockdown abolishes Notch-induced Slug expression. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. PMID:18663143 Dll4-mediated induction of Slug mRNA and protein was dramatically reduced when CSL was knocked down using either of two lentiviral-delivered short hairpin RNA (shRNA) constructs |
| GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific | IEA GO_REF:0000120 | ACCEPT | Summary: In complex with NICD/MAML, RBPJ activates targets such as Slug/SNAI2, HEY genes and COX4I2. Reason: Activator activity is a core state of RBPJ; CSL knockdown abolishes Notch-induced Slug expression. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. PMID:18663143 Dll4-mediated induction of Slug mRNA and protein was dramatically reduced when CSL was knocked down using either of two lentiviral-delivered short hairpin RNA (shRNA) constructs |
| GO:0001525 angiogenesis | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Vascular development roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0001837 epithelial to mesenchymal transition | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0001974 blood vessel remodeling | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Vascular development roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0001974 blood vessel remodeling | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Vascular development roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0002193 MAML1-RBP-Jkappa- ICN1 complex | IBA GO_REF:0000033 | ACCEPT | Summary: RBPJ is the DNA-binding core of the CSL-NICD-MAML ternary activation complex. Reason: Structurally and biochemically defined core complex (PTN002580211 for the IBA). Supporting Evidence: PMID:16530044 We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). PMID:19907488 We demonstrate that direct, high-affinity binding of the hydrocarbon-stapled peptide SAHM1 prevents assembly of the active transcriptional complex. PMID:16510869 MAML1's promyogenic effects were completely blocked upon activation of Notch signaling, which was associated with recruitment of MAML1 away from MEF2C to the Notch transcriptional complex. |
| GO:0002193 MAML1-RBP-Jkappa- ICN1 complex | IDA PMID:16510869 The Notch coactivator, MAML1, functions as a novel coactivat... | ACCEPT | Summary: RBPJ is the DNA-binding core of the CSL-NICD-MAML ternary activation complex. Reason: Structurally and biochemically defined core complex (PTN002580211 for the IBA). Supporting Evidence: PMID:16530044 We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). PMID:19907488 We demonstrate that direct, high-affinity binding of the hydrocarbon-stapled peptide SAHM1 prevents assembly of the active transcriptional complex. PMID:16510869 MAML1's promyogenic effects were completely blocked upon activation of Notch signaling, which was associated with recruitment of MAML1 away from MEF2C to the Notch transcriptional complex. |
| GO:0003151 outflow tract morphogenesis | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0003151 outflow tract morphogenesis | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0003157 endocardium development | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0003160 endocardium morphogenesis | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0003160 endocardium morphogenesis | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0003176 aortic valve development | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0003176 aortic valve development | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0003177 pulmonary valve development | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0003177 pulmonary valve development | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0003198 epithelial to mesenchymal transition involved in endocardial cushion formation | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0003198 epithelial to mesenchymal transition involved in endocardial cushion formation | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0003214 cardiac left ventricle morphogenesis | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0003214 cardiac left ventricle morphogenesis | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0003222 ventricular trabecula myocardium morphogenesis | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0003222 ventricular trabecula myocardium morphogenesis | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0003677 DNA binding | IEA GO_REF:0000120 | ACCEPT | Summary: DNA binding. Reason: Correct, less specific than GO:0000978. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. |
| GO:0003677 DNA binding | TAS PMID:9874765 CIR, a corepressor linking the DNA binding factor CBF1 to th... | ACCEPT | Summary: DNA binding. Reason: Correct, less specific than GO:0000978. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. |
| GO:0003682 chromatin binding | IEA GO_REF:0000107 | ACCEPT | Summary: Chromatin binding (mouse orthology). Reason: RBPJ binds target enhancers in chromatin; consistent. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. |
| GO:0003700 DNA-binding transcription factor activity | IEA GO_REF:0000002 | ACCEPT | Summary: RBPJ is a DNA-binding transcription factor that represses or activates RNA polymerase II targets. Reason: Core MF of CSL; supported by phylogeny (PTN000071433), TFClass and experiments. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Acts as a transcriptional repressor when it is not associated with Notch proteins. file:human/RBPJ/RBPJ-uniprot.txt When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. |
| GO:0003700 DNA-binding transcription factor activity | TAS PMID:9874765 CIR, a corepressor linking the DNA binding factor CBF1 to th... | ACCEPT | Summary: RBPJ is a DNA-binding transcription factor that represses or activates RNA polymerase II targets. Reason: Core MF of CSL; supported by phylogeny (PTN000071433), TFClass and experiments. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Acts as a transcriptional repressor when it is not associated with Notch proteins. file:human/RBPJ/RBPJ-uniprot.txt When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. |
| GO:0005515 protein binding | IPI PMID:10713164 SKIP, a CBF1-associated protein, interacts with the ankyrin ... | MODIFY | Summary: RBPJ binds the NOTCH1 intracellular domain (NICD), the coactivator that converts it into an activator. Reason: Generic protein binding. NICD acts as a transcriptional coactivator for DNA-bound RBPJ; transcription coactivator binding is the informative MF (complex membership is captured by GO:1990433/GO:0002193). Proposed replacements: transcription coactivator binding Supporting Evidence: PMID:16530044 We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). file:human/RBPJ/RBPJ-uniprot.txt Interacts with activated NOTCH1, NOTCH2 or NOTCH3. |
| GO:0005515 protein binding | IPI PMID:10713164 SKIP, a CBF1-associated protein, interacts with the ankyrin ... | MODIFY | Summary: SKIP/SNW1 binds CBF1 and NotchIC and is required for NotchIC activity. Reason: Generic protein binding; SNW1 acts as a coactivator of NotchIC-RBPJ. Proposed replacements: transcription coactivator binding Supporting Evidence: PMID:10713164 We identified SKIP (Ski-interacting protein) as a CBF1 binding protein in a yeast two-hybrid screen. PMID:10713164 The results suggest a model in which NotchIC activates responsive promoters by competing with the SMRT-corepre |
| GO:0005515 protein binding | IPI PMID:10713164 SKIP, a CBF1-associated protein, interacts with the ankyrin ... | MODIFY | Summary: Binding of RBPJ to corepressors (NCOR2/SMRT, SPEN/SHARP, CIR, L3MBTL3, ATXN1/ATXN1L, KDM1A/LSD1). Reason: Generic protein binding; these partners are corepressors recruited by RBPJ to repress Notch targets in the absence of NICD, so transcription corepressor binding is the informative MF. Proposed replacements: transcription corepressor binding Supporting Evidence: PMID:10713164 CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex. PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. PMID:9874765 We have examined the mechanism of CBF1-mediated repression and show that CBF1 binds to a unique corepressor, CBF1 interacting corepressor (CIR). PMID:29030483 In the absence of NOTCH ICD, RBPJ recruits L3MBTL3 and the histone demethylase KDM1A (also known as LSD1) to the enhancers of Notch target genes, leading to H3K4me2 demethylation and to transcriptional repression. PMID:21475249 both ATXN1 and BOAT1 bind to the promoter region of Hey1 and inhibit the transcriptional output of Notch through direct interactions with CBF1 PMID:23022380 LSD1 functions as a corepressor when associated with CSL-repressor complex and as a NOTCH1 coactivator upon Notch activation. |
| GO:0005515 protein binding | IPI PMID:16510869 The Notch coactivator, MAML1, functions as a novel coactivat... | MODIFY | Summary: RBPJ binds the NOTCH1 intracellular domain (NICD), the coactivator that converts it into an activator. Reason: Generic protein binding. NICD acts as a transcriptional coactivator for DNA-bound RBPJ; transcription coactivator binding is the informative MF (complex membership is captured by GO:1990433/GO:0002193). Proposed replacements: transcription coactivator binding Supporting Evidence: PMID:16530044 We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). file:human/RBPJ/RBPJ-uniprot.txt Interacts with activated NOTCH1, NOTCH2 or NOTCH3. |
| GO:0005515 protein binding | IPI PMID:16530044 Structural basis for cooperativity in recruitment of MAML co... | MODIFY | Summary: Crystal structure of CSL-NICD-MAML1 on DNA. Reason: Generic protein binding; MAML1 is a transcription coactivator bound in the CSL/ANK groove. Proposed replacements: transcription coactivator binding Supporting Evidence: PMID:16530044 Together, CSL and ANK create a groove to bind the MAML-1 polypeptide as a kinked, 70 A helix. |
| GO:0005515 protein binding | IPI PMID:17284587 Cooperative assembly of higher-order Notch complexes functio... | MODIFY | Summary: RBPJ binds the NOTCH1 intracellular domain (NICD), the coactivator that converts it into an activator. Reason: Generic protein binding. NICD acts as a transcriptional coactivator for DNA-bound RBPJ; transcription coactivator binding is the informative MF (complex membership is captured by GO:1990433/GO:0002193). Proposed replacements: transcription coactivator binding Supporting Evidence: PMID:16530044 We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). file:human/RBPJ/RBPJ-uniprot.txt Interacts with activated NOTCH1, NOTCH2 or NOTCH3. |
| GO:0005515 protein binding | IPI PMID:21102556 RITA, a novel modulator of Notch signalling, acts via nuclea... | REMOVE | Summary: RITA1 binds RBPJ and exports it from the nucleus. Reason: Generic protein binding; the functional content (nuclear export as a negative modulation of Notch) is a property of RITA1. Interaction not disputed. Supporting Evidence: PMID:21102556 This shuttling RITA exports RBP-J/CBF-1 from the nucleus. |
| GO:0005515 protein binding | IPI PMID:21102556 RITA, a novel modulator of Notch signalling, acts via nuclea... | MODIFY | Summary: RBPJ binds the NOTCH1 intracellular domain (NICD), the coactivator that converts it into an activator. Reason: Generic protein binding. NICD acts as a transcriptional coactivator for DNA-bound RBPJ; transcription coactivator binding is the informative MF (complex membership is captured by GO:1990433/GO:0002193). Proposed replacements: transcription coactivator binding Supporting Evidence: PMID:16530044 We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). file:human/RBPJ/RBPJ-uniprot.txt Interacts with activated NOTCH1, NOTCH2 or NOTCH3. |
| GO:0005515 protein binding | IPI PMID:21102556 RITA, a novel modulator of Notch signalling, acts via nuclea... | REMOVE | Summary: RITA1 binds RBPJ and exports it from the nucleus. Reason: Generic protein binding; the functional content (nuclear export as a negative modulation of Notch) is a property of RITA1. Interaction not disputed. Supporting Evidence: PMID:21102556 This shuttling RITA exports RBP-J/CBF-1 from the nucleus. |
| GO:0005515 protein binding | IPI PMID:21102556 RITA, a novel modulator of Notch signalling, acts via nuclea... | MODIFY | Summary: Binding of RBPJ to corepressors (NCOR2/SMRT, SPEN/SHARP, CIR, L3MBTL3, ATXN1/ATXN1L, KDM1A/LSD1). Reason: Generic protein binding; these partners are corepressors recruited by RBPJ to repress Notch targets in the absence of NICD, so transcription corepressor binding is the informative MF. Proposed replacements: transcription corepressor binding Supporting Evidence: PMID:10713164 CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex. PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. PMID:9874765 We have examined the mechanism of CBF1-mediated repression and show that CBF1 binds to a unique corepressor, CBF1 interacting corepressor (CIR). PMID:29030483 In the absence of NOTCH ICD, RBPJ recruits L3MBTL3 and the histone demethylase KDM1A (also known as LSD1) to the enhancers of Notch target genes, leading to H3K4me2 demethylation and to transcriptional repression. PMID:21475249 both ATXN1 and BOAT1 bind to the promoter region of Hey1 and inhibit the transcriptional output of Notch through direct interactions with CBF1 PMID:23022380 LSD1 functions as a corepressor when associated with CSL-repressor complex and as a NOTCH1 coactivator upon Notch activation. |
| GO:0005515 protein binding | IPI PMID:21475249 Ataxin-1 and Brother of ataxin-1 are components of the Notch... | MODIFY | Summary: Binding of RBPJ to corepressors (NCOR2/SMRT, SPEN/SHARP, CIR, L3MBTL3, ATXN1/ATXN1L, KDM1A/LSD1). Reason: Generic protein binding; these partners are corepressors recruited by RBPJ to repress Notch targets in the absence of NICD, so transcription corepressor binding is the informative MF. Proposed replacements: transcription corepressor binding Supporting Evidence: PMID:10713164 CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex. PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. PMID:9874765 We have examined the mechanism of CBF1-mediated repression and show that CBF1 binds to a unique corepressor, CBF1 interacting corepressor (CIR). PMID:29030483 In the absence of NOTCH ICD, RBPJ recruits L3MBTL3 and the histone demethylase KDM1A (also known as LSD1) to the enhancers of Notch target genes, leading to H3K4me2 demethylation and to transcriptional repression. PMID:21475249 both ATXN1 and BOAT1 bind to the promoter region of Hey1 and inhibit the transcriptional output of Notch through direct interactions with CBF1 PMID:23022380 LSD1 functions as a corepressor when associated with CSL-repressor complex and as a NOTCH1 coactivator upon Notch activation. |
| GO:0005515 protein binding | IPI PMID:21475249 Ataxin-1 and Brother of ataxin-1 are components of the Notch... | MODIFY | Summary: RBPJ binds the NOTCH1 intracellular domain (NICD), the coactivator that converts it into an activator. Reason: Generic protein binding. NICD acts as a transcriptional coactivator for DNA-bound RBPJ; transcription coactivator binding is the informative MF (complex membership is captured by GO:1990433/GO:0002193). Proposed replacements: transcription coactivator binding Supporting Evidence: PMID:16530044 We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). file:human/RBPJ/RBPJ-uniprot.txt Interacts with activated NOTCH1, NOTCH2 or NOTCH3. |
| GO:0005515 protein binding | IPI PMID:21475249 Ataxin-1 and Brother of ataxin-1 are components of the Notch... | MODIFY | Summary: Binding of RBPJ to corepressors (NCOR2/SMRT, SPEN/SHARP, CIR, L3MBTL3, ATXN1/ATXN1L, KDM1A/LSD1). Reason: Generic protein binding; these partners are corepressors recruited by RBPJ to repress Notch targets in the absence of NICD, so transcription corepressor binding is the informative MF. Proposed replacements: transcription corepressor binding Supporting Evidence: PMID:10713164 CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex. PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. PMID:9874765 We have examined the mechanism of CBF1-mediated repression and show that CBF1 binds to a unique corepressor, CBF1 interacting corepressor (CIR). PMID:29030483 In the absence of NOTCH ICD, RBPJ recruits L3MBTL3 and the histone demethylase KDM1A (also known as LSD1) to the enhancers of Notch target genes, leading to H3K4me2 demethylation and to transcriptional repression. PMID:21475249 both ATXN1 and BOAT1 bind to the promoter region of Hey1 and inhibit the transcriptional output of Notch through direct interactions with CBF1 PMID:23022380 LSD1 functions as a corepressor when associated with CSL-repressor complex and as a NOTCH1 coactivator upon Notch activation. |
| GO:0005515 protein binding | IPI PMID:21475249 Ataxin-1 and Brother of ataxin-1 are components of the Notch... | MODIFY | Summary: Binding of RBPJ to corepressors (NCOR2/SMRT, SPEN/SHARP, CIR, L3MBTL3, ATXN1/ATXN1L, KDM1A/LSD1). Reason: Generic protein binding; these partners are corepressors recruited by RBPJ to repress Notch targets in the absence of NICD, so transcription corepressor binding is the informative MF. Proposed replacements: transcription corepressor binding Supporting Evidence: PMID:10713164 CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex. PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. PMID:9874765 We have examined the mechanism of CBF1-mediated repression and show that CBF1 binds to a unique corepressor, CBF1 interacting corepressor (CIR). PMID:29030483 In the absence of NOTCH ICD, RBPJ recruits L3MBTL3 and the histone demethylase KDM1A (also known as LSD1) to the enhancers of Notch target genes, leading to H3K4me2 demethylation and to transcriptional repression. PMID:21475249 both ATXN1 and BOAT1 bind to the promoter region of Hey1 and inhibit the transcriptional output of Notch through direct interactions with CBF1 PMID:23022380 LSD1 functions as a corepressor when associated with CSL-repressor complex and as a NOTCH1 coactivator upon Notch activation. |
| GO:0005515 protein binding | IPI PMID:22810586 Interpreting cancer genomes using systematic host network pe... | REMOVE | Summary: Binding of Epstein-Barr virus EBNA2/EBNA3 proteins to RBPJ. Reason: Generic protein binding; viral hijacking of RBPJ. Uninformative about RBPJ's own MF; interaction not disputed. Supporting Evidence: PMID:8627785 EBNA3A and EBNA3B can also inhibit the interaction of RBPJkappa with cognate DNA in vitro. |
| GO:0005515 protein binding | IPI PMID:23022380 NOTCH1 nuclear interactome reveals key regulators of its tra... | MODIFY | Summary: Binding of RBPJ to corepressors (NCOR2/SMRT, SPEN/SHARP, CIR, L3MBTL3, ATXN1/ATXN1L, KDM1A/LSD1). Reason: Generic protein binding; these partners are corepressors recruited by RBPJ to repress Notch targets in the absence of NICD, so transcription corepressor binding is the informative MF. Proposed replacements: transcription corepressor binding Supporting Evidence: PMID:10713164 CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex. PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. PMID:9874765 We have examined the mechanism of CBF1-mediated repression and show that CBF1 binds to a unique corepressor, CBF1 interacting corepressor (CIR). PMID:29030483 In the absence of NOTCH ICD, RBPJ recruits L3MBTL3 and the histone demethylase KDM1A (also known as LSD1) to the enhancers of Notch target genes, leading to H3K4me2 demethylation and to transcriptional repression. PMID:21475249 both ATXN1 and BOAT1 bind to the promoter region of Hey1 and inhibit the transcriptional output of Notch through direct interactions with CBF1 PMID:23022380 LSD1 functions as a corepressor when associated with CSL-repressor complex and as a NOTCH1 coactivator upon Notch activation. |
| GO:0005515 protein binding | IPI PMID:23022380 NOTCH1 nuclear interactome reveals key regulators of its tra... | MODIFY | Summary: RBPJ binds the NOTCH1 intracellular domain (NICD), the coactivator that converts it into an activator. Reason: Generic protein binding. NICD acts as a transcriptional coactivator for DNA-bound RBPJ; transcription coactivator binding is the informative MF (complex membership is captured by GO:1990433/GO:0002193). Proposed replacements: transcription coactivator binding Supporting Evidence: PMID:16530044 We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). file:human/RBPJ/RBPJ-uniprot.txt Interacts with activated NOTCH1, NOTCH2 or NOTCH3. |
| GO:0005515 protein binding | IPI PMID:23022380 NOTCH1 nuclear interactome reveals key regulators of its tra... | REMOVE | Summary: Proteomic co-purification with SMARCA4, PHF8 or FBXO42. Reason: Generic protein binding from interactome studies (likely indirect, within NICD complexes); uninformative. Supporting Evidence: PMID:23022380 LSD1 functions as a corepressor when associated with CSL-repressor complex and as a NOTCH1 coactivator upon Notch activation. |
| GO:0005515 protein binding | IPI PMID:23022380 NOTCH1 nuclear interactome reveals key regulators of its tra... | REMOVE | Summary: Proteomic co-purification with SMARCA4, PHF8 or FBXO42. Reason: Generic protein binding from interactome studies (likely indirect, within NICD complexes); uninformative. Supporting Evidence: PMID:23022380 LSD1 functions as a corepressor when associated with CSL-repressor complex and as a NOTCH1 coactivator upon Notch activation. |
| GO:0005515 protein binding | IPI PMID:25609649 Proteomic analyses reveal distinct chromatin-associated and ... | MODIFY | Summary: Binding of RBPJ to corepressors (NCOR2/SMRT, SPEN/SHARP, CIR, L3MBTL3, ATXN1/ATXN1L, KDM1A/LSD1). Reason: Generic protein binding; these partners are corepressors recruited by RBPJ to repress Notch targets in the absence of NICD, so transcription corepressor binding is the informative MF. Proposed replacements: transcription corepressor binding Supporting Evidence: PMID:10713164 CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex. PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. PMID:9874765 We have examined the mechanism of CBF1-mediated repression and show that CBF1 binds to a unique corepressor, CBF1 interacting corepressor (CIR). PMID:29030483 In the absence of NOTCH ICD, RBPJ recruits L3MBTL3 and the histone demethylase KDM1A (also known as LSD1) to the enhancers of Notch target genes, leading to H3K4me2 demethylation and to transcriptional repression. PMID:21475249 both ATXN1 and BOAT1 bind to the promoter region of Hey1 and inhibit the transcriptional output of Notch through direct interactions with CBF1 PMID:23022380 LSD1 functions as a corepressor when associated with CSL-repressor complex and as a NOTCH1 coactivator upon Notch activation. |
| GO:0005515 protein binding | IPI PMID:25609649 Proteomic analyses reveal distinct chromatin-associated and ... | MODIFY | Summary: RBPJ binds the NOTCH1 intracellular domain (NICD), the coactivator that converts it into an activator. Reason: Generic protein binding. NICD acts as a transcriptional coactivator for DNA-bound RBPJ; transcription coactivator binding is the informative MF (complex membership is captured by GO:1990433/GO:0002193). Proposed replacements: transcription coactivator binding Supporting Evidence: PMID:16530044 We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). file:human/RBPJ/RBPJ-uniprot.txt Interacts with activated NOTCH1, NOTCH2 or NOTCH3. |
| GO:0005515 protein binding | IPI PMID:25609649 Proteomic analyses reveal distinct chromatin-associated and ... | REMOVE | Summary: Proteomic co-purification with SMARCA4, PHF8 or FBXO42. Reason: Generic protein binding from interactome studies (likely indirect, within NICD complexes); uninformative. Supporting Evidence: PMID:23022380 LSD1 functions as a corepressor when associated with CSL-repressor complex and as a NOTCH1 coactivator upon Notch activation. |
| GO:0005515 protein binding | IPI PMID:25609649 Proteomic analyses reveal distinct chromatin-associated and ... | REMOVE | Summary: RITA1 binds RBPJ and exports it from the nucleus. Reason: Generic protein binding; the functional content (nuclear export as a negative modulation of Notch) is a property of RITA1. Interaction not disputed. Supporting Evidence: PMID:21102556 This shuttling RITA exports RBP-J/CBF-1 from the nucleus. |
| GO:0005515 protein binding | IPI PMID:25609649 Proteomic analyses reveal distinct chromatin-associated and ... | MODIFY | Summary: Binding of RBPJ to corepressors (NCOR2/SMRT, SPEN/SHARP, CIR, L3MBTL3, ATXN1/ATXN1L, KDM1A/LSD1). Reason: Generic protein binding; these partners are corepressors recruited by RBPJ to repress Notch targets in the absence of NICD, so transcription corepressor binding is the informative MF. Proposed replacements: transcription corepressor binding Supporting Evidence: PMID:10713164 CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex. PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. PMID:9874765 We have examined the mechanism of CBF1-mediated repression and show that CBF1 binds to a unique corepressor, CBF1 interacting corepressor (CIR). PMID:29030483 In the absence of NOTCH ICD, RBPJ recruits L3MBTL3 and the histone demethylase KDM1A (also known as LSD1) to the enhancers of Notch target genes, leading to H3K4me2 demethylation and to transcriptional repression. PMID:21475249 both ATXN1 and BOAT1 bind to the promoter region of Hey1 and inhibit the transcriptional output of Notch through direct interactions with CBF1 PMID:23022380 LSD1 functions as a corepressor when associated with CSL-repressor complex and as a NOTCH1 coactivator upon Notch activation. |
| GO:0005515 protein binding | IPI PMID:26302407 Combined CSL and p53 downregulation promotes cancer-associat... | REMOVE | Summary: RBPJ-p53 interaction in fibroblasts. Reason: Generic protein binding; uninformative. Supporting Evidence: PMID:26302407 It also physically interacts with p53, repressing its activity. |
| GO:0005515 protein binding | IPI PMID:26496610 A human interactome in three quantitative dimensions organiz... | MODIFY | Summary: RBPJ binds the NOTCH1 intracellular domain (NICD), the coactivator that converts it into an activator. Reason: Generic protein binding. NICD acts as a transcriptional coactivator for DNA-bound RBPJ; transcription coactivator binding is the informative MF (complex membership is captured by GO:1990433/GO:0002193). Proposed replacements: transcription coactivator binding Supporting Evidence: PMID:16530044 We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). file:human/RBPJ/RBPJ-uniprot.txt Interacts with activated NOTCH1, NOTCH2 or NOTCH3. |
| GO:0005515 protein binding | IPI PMID:29030483 RBPJ/CBF1 interacts with L3MBTL3/MBT1 to promote repression ... | MODIFY | Summary: Binding of RBPJ to corepressors (NCOR2/SMRT, SPEN/SHARP, CIR, L3MBTL3, ATXN1/ATXN1L, KDM1A/LSD1). Reason: Generic protein binding; these partners are corepressors recruited by RBPJ to repress Notch targets in the absence of NICD, so transcription corepressor binding is the informative MF. Proposed replacements: transcription corepressor binding Supporting Evidence: PMID:10713164 CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex. PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. PMID:9874765 We have examined the mechanism of CBF1-mediated repression and show that CBF1 binds to a unique corepressor, CBF1 interacting corepressor (CIR). PMID:29030483 In the absence of NOTCH ICD, RBPJ recruits L3MBTL3 and the histone demethylase KDM1A (also known as LSD1) to the enhancers of Notch target genes, leading to H3K4me2 demethylation and to transcriptional repression. PMID:21475249 both ATXN1 and BOAT1 bind to the promoter region of Hey1 and inhibit the transcriptional output of Notch through direct interactions with CBF1 PMID:23022380 LSD1 functions as a corepressor when associated with CSL-repressor complex and as a NOTCH1 coactivator upon Notch activation. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MODIFY | Summary: RBPJ binds the NOTCH1 intracellular domain (NICD), the coactivator that converts it into an activator. Reason: Generic protein binding. NICD acts as a transcriptional coactivator for DNA-bound RBPJ; transcription coactivator binding is the informative MF (complex membership is captured by GO:1990433/GO:0002193). Proposed replacements: transcription coactivator binding Supporting Evidence: PMID:16530044 We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). file:human/RBPJ/RBPJ-uniprot.txt Interacts with activated NOTCH1, NOTCH2 or NOTCH3. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | REMOVE | Summary: Proteomic co-purification with SMARCA4, PHF8 or FBXO42. Reason: Generic protein binding from interactome studies (likely indirect, within NICD complexes); uninformative. Supporting Evidence: PMID:23022380 LSD1 functions as a corepressor when associated with CSL-repressor complex and as a NOTCH1 coactivator upon Notch activation. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MODIFY | Summary: Binding of RBPJ to corepressors (NCOR2/SMRT, SPEN/SHARP, CIR, L3MBTL3, ATXN1/ATXN1L, KDM1A/LSD1). Reason: Generic protein binding; these partners are corepressors recruited by RBPJ to repress Notch targets in the absence of NICD, so transcription corepressor binding is the informative MF. Proposed replacements: transcription corepressor binding Supporting Evidence: PMID:10713164 CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex. PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. PMID:9874765 We have examined the mechanism of CBF1-mediated repression and show that CBF1 binds to a unique corepressor, CBF1 interacting corepressor (CIR). PMID:29030483 In the absence of NOTCH ICD, RBPJ recruits L3MBTL3 and the histone demethylase KDM1A (also known as LSD1) to the enhancers of Notch target genes, leading to H3K4me2 demethylation and to transcriptional repression. PMID:21475249 both ATXN1 and BOAT1 bind to the promoter region of Hey1 and inhibit the transcriptional output of Notch through direct interactions with CBF1 PMID:23022380 LSD1 functions as a corepressor when associated with CSL-repressor complex and as a NOTCH1 coactivator upon Notch activation. |
| GO:0005515 protein binding | IPI PMID:41086914 Pathogenic variants in KCTD1 disrupt cAMP signaling and cell... | REMOVE | Summary: KCTD1 interaction. Reason: Generic protein binding; uninformative. Supporting Evidence: PMID:41086914 We further identified that KCTD1 interacts with the Notch transcriptional repressor RBPJ. |
| GO:0005515 protein binding | IPI PMID:8627785 The amino-terminal domains of Epstein-Barr virus nuclear pro... | REMOVE | Summary: Binding of Epstein-Barr virus EBNA2/EBNA3 proteins to RBPJ. Reason: Generic protein binding; viral hijacking of RBPJ. Uninformative about RBPJ's own MF; interaction not disputed. Supporting Evidence: PMID:8627785 EBNA3A and EBNA3B can also inhibit the interaction of RBPJkappa with cognate DNA in vitro. |
| GO:0005515 protein binding | IPI PMID:8627785 The amino-terminal domains of Epstein-Barr virus nuclear pro... | REMOVE | Summary: Binding of Epstein-Barr virus EBNA2/EBNA3 proteins to RBPJ. Reason: Generic protein binding; viral hijacking of RBPJ. Uninformative about RBPJ's own MF; interaction not disputed. Supporting Evidence: PMID:8627785 EBNA3A and EBNA3B can also inhibit the interaction of RBPJkappa with cognate DNA in vitro. |
| GO:0005515 protein binding | IPI PMID:8627785 The amino-terminal domains of Epstein-Barr virus nuclear pro... | REMOVE | Summary: Binding of Epstein-Barr virus EBNA2/EBNA3 proteins to RBPJ. Reason: Generic protein binding; viral hijacking of RBPJ. Uninformative about RBPJ's own MF; interaction not disputed. Supporting Evidence: PMID:8627785 EBNA3A and EBNA3B can also inhibit the interaction of RBPJkappa with cognate DNA in vitro. |
| GO:0005515 protein binding | IPI PMID:8627785 The amino-terminal domains of Epstein-Barr virus nuclear pro... | REMOVE | Summary: Binding of Epstein-Barr virus EBNA2/EBNA3 proteins to RBPJ. Reason: Generic protein binding; viral hijacking of RBPJ. Uninformative about RBPJ's own MF; interaction not disputed. Supporting Evidence: PMID:8627785 EBNA3A and EBNA3B can also inhibit the interaction of RBPJkappa with cognate DNA in vitro. |
| GO:0005515 protein binding | IPI PMID:9874765 CIR, a corepressor linking the DNA binding factor CBF1 to th... | MODIFY | Summary: Binding of RBPJ to corepressors (NCOR2/SMRT, SPEN/SHARP, CIR, L3MBTL3, ATXN1/ATXN1L, KDM1A/LSD1). Reason: Generic protein binding; these partners are corepressors recruited by RBPJ to repress Notch targets in the absence of NICD, so transcription corepressor binding is the informative MF. Proposed replacements: transcription corepressor binding Supporting Evidence: PMID:10713164 CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex. PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. PMID:9874765 We have examined the mechanism of CBF1-mediated repression and show that CBF1 binds to a unique corepressor, CBF1 interacting corepressor (CIR). PMID:29030483 In the absence of NOTCH ICD, RBPJ recruits L3MBTL3 and the histone demethylase KDM1A (also known as LSD1) to the enhancers of Notch target genes, leading to H3K4me2 demethylation and to transcriptional repression. PMID:21475249 both ATXN1 and BOAT1 bind to the promoter region of Hey1 and inhibit the transcriptional output of Notch through direct interactions with CBF1 PMID:23022380 LSD1 functions as a corepressor when associated with CSL-repressor complex and as a NOTCH1 coactivator upon Notch activation. |
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005634 nucleus | IDA PMID:21102556 RITA, a novel modulator of Notch signalling, acts via nuclea... | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005634 nucleus | IDA PMID:32601208 Pharmacological disruption of the Notch transcription factor... | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005634 nucleus | IDA PMID:9874765 CIR, a corepressor linking the DNA binding factor CBF1 to th... | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005634 nucleus | IEA GO_REF:0000120 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | IDA GO_REF:0000052 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-1912394 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-212356 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-2197588 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-2220957 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-2220964 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-2220971 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-2220982 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-2976742 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-350058 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-4396371 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-4396379 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-4396392 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-4396393 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-4396401 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-4396402 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-8878220 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-8878237 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9017835 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9018542 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9021406 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9021451 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-HSA-9021480 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-NUL-2064264 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-NUL-2064916 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-NUL-2065178 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-NUL-4396363 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-NUL-9013647 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-NUL-9013660 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-NUL-9013699 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005654 nucleoplasm | TAS Reactome:R-NUL-9604511 | ACCEPT | Summary: Nuclear localization of RBPJ. Reason: RBPJ is mainly nuclear where it binds target genes. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0005667 transcription regulator complex | IEA GO_REF:0000120 | ACCEPT | Summary: Transcription regulator complex. Reason: Consistent with activator and corepressor complexes. Supporting Evidence: PMID:16530044 We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). |
| GO:0005730 nucleolus | IDA PMID:9874765 CIR, a corepressor linking the DNA binding factor CBF1 to th... | KEEP AS NON CORE | Summary: Nucleolar staining reported in the CIR study. Reason: Minor localization; not a site of core function. Supporting Evidence: PMID:9874765 We have examined the mechanism of CBF1-mediated repression and show that CBF1 binds to a unique corepressor, CBF1 interacting corepressor (CIR). |
| GO:0005737 cytoplasm | IDA PMID:21102556 RITA, a novel modulator of Notch signalling, acts via nuclea... | KEEP AS NON CORE | Summary: Cytoplasmic RBPJ after RITA1-mediated export. Reason: Regulatory relocalization that removes RBPJ from its site of action. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 This shuttling RITA exports RBP-J/CBF-1 from the nucleus. |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Cytoplasmic RBPJ after RITA1-mediated export. Reason: Regulatory relocalization that removes RBPJ from its site of action. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm PMID:21102556 This shuttling RITA exports RBP-J/CBF-1 from the nucleus. |
| GO:0006355 regulation of DNA-templated transcription | IEA GO_REF:0000002 | ACCEPT | Summary: Regulation of transcription. Reason: General but correct. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0006357 regulation of transcription by RNA polymerase II | IEA GO_REF:0000120 | ACCEPT | Summary: Regulation of transcription. Reason: General but correct. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0006357 regulation of transcription by RNA polymerase II | ISS GO_REF:0000024 | ACCEPT | Summary: Regulation of transcription. Reason: General but correct. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0007219 Notch signaling pathway | IBA GO_REF:0000033 | ACCEPT | Summary: RBPJ is the nuclear effector of canonical Notch signaling. Reason: Core process (PTN002580211 for IBA); direct evidence from complex-disrupting inhibitors and knockdown. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling PMID:32601208 we report the discovery of an orally active small-molecule inhibitor (termed CB-103) of the Notch transcription activation complex. PMID:18663143 Dll4-mediated induction of Slug mRNA and protein was dramatically reduced when CSL was knocked down using either of two lentiviral-delivered short hairpin RNA (shRNA) constructs |
| GO:0007219 Notch signaling pathway | IDA PMID:32601208 Pharmacological disruption of the Notch transcription factor... | ACCEPT | Summary: RBPJ is the nuclear effector of canonical Notch signaling. Reason: Core process (PTN002580211 for IBA); direct evidence from complex-disrupting inhibitors and knockdown. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling PMID:32601208 we report the discovery of an orally active small-molecule inhibitor (termed CB-103) of the Notch transcription activation complex. PMID:18663143 Dll4-mediated induction of Slug mRNA and protein was dramatically reduced when CSL was knocked down using either of two lentiviral-delivered short hairpin RNA (shRNA) constructs |
| GO:0007219 Notch signaling pathway | IMP PMID:18663143 Slug is a direct Notch target required for initiation of car... | ACCEPT | Summary: RBPJ is the nuclear effector of canonical Notch signaling. Reason: Core process (PTN002580211 for IBA); direct evidence from complex-disrupting inhibitors and knockdown. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling PMID:32601208 we report the discovery of an orally active small-molecule inhibitor (termed CB-103) of the Notch transcription activation complex. PMID:18663143 Dll4-mediated induction of Slug mRNA and protein was dramatically reduced when CSL was knocked down using either of two lentiviral-delivered short hairpin RNA (shRNA) constructs |
| GO:0007219 Notch signaling pathway | IMP PMID:21311046 Differential Notch signaling in the epicardium is required f... | ACCEPT | Summary: RBPJ is the nuclear effector of canonical Notch signaling. Reason: Core process (PTN002580211 for IBA); direct evidence from complex-disrupting inhibitors and knockdown. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling PMID:32601208 we report the discovery of an orally active small-molecule inhibitor (termed CB-103) of the Notch transcription activation complex. PMID:18663143 Dll4-mediated induction of Slug mRNA and protein was dramatically reduced when CSL was knocked down using either of two lentiviral-delivered short hairpin RNA (shRNA) constructs |
| GO:0007221 positive regulation of transcription of Notch receptor target | IDA PMID:21102556 RITA, a novel modulator of Notch signalling, acts via nuclea... | ACCEPT | Summary: Positive regulation of transcription of Notch receptor targets. Reason: The most precise BP for RBPJ's activator role. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0007221 positive regulation of transcription of Notch receptor target | IEA GO_REF:0000117 | ACCEPT | Summary: Positive regulation of transcription of Notch receptor targets. Reason: The most precise BP for RBPJ's activator role. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. PMID:21102556 In the nucleus, NICD binds to the recombination signal sequence-binding protein J (RBP-J)/CBF-1 transcription factor to induce expression of Notch target genes. |
| GO:0007507 heart development | IMP PMID:21311046 Differential Notch signaling in the epicardium is required f... | KEEP AS NON CORE | Summary: Rbpj-targeted mouse embryos show proepicardial/inflow defects. Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:21311046 Analysis of RBPJk-targeted embryos indicates that Notch ablation causes ectopic procardiogenic signaling in the proepicardium |
| GO:0017053 transcription repressor complex | IDA PMID:16691198 A metabolic enzyme of the short-chain dehydrogenase/reductas... | ACCEPT | Summary: RBPJ in corepressor-containing repressor complexes. Reason: RBPJ-SHARP/SMRT/CIR corepressor complexes are well established; accepted (the specific cited study concerns hRoDH-E2). Supporting Evidence: PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. PMID:9874765 We have examined the mechanism of CBF1-mediated repression and show that CBF1 binds to a unique corepressor, CBF1 interacting corepressor (CIR). |
| GO:0030279 negative regulation of ossification | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Negative regulation of ossification (mouse). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0030279 negative regulation of ossification | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Negative regulation of ossification (mouse). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0030513 positive regulation of BMP signaling pathway | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Positive regulation of BMP/ERBB/ephrin signaling (mouse Rbpj). Reason: Cross-pathway effects of Notch target gene expression in cardiac development; indirect for RBPJ. Supporting Evidence: PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0030513 positive regulation of BMP signaling pathway | ISS GO_REF:0000024 | MARK AS OVER ANNOTATED | Summary: Positive regulation of BMP/ERBB/ephrin signaling (mouse Rbpj). Reason: Cross-pathway effects of Notch target gene expression in cardiac development; indirect for RBPJ. Supporting Evidence: PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0035912 dorsal aorta morphogenesis | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Vascular development roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0035912 dorsal aorta morphogenesis | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Vascular development roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0036302 atrioventricular canal development | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0036302 atrioventricular canal development | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0043467 regulation of generation of precursor metabolites and energy | IDA PMID:23303788 Oxygen-dependent expression of cytochrome c oxidase subunit ... | MARK AS OVER ANNOTATED | Summary: RBPJ activates COX4I2 transcription under 4% oxygen. Reason: The direct activity is transcriptional activation of one gene; a metabolic-energy process term overstates it. Supporting Evidence: PMID:23303788 We demonstrate that RBPJ and CHCHD2 function towards activating the ORE at 4% oxygen, whereas CXXC5 functions as an inhibitor. |
| GO:0043565 sequence-specific DNA binding | IDA PMID:23303788 Oxygen-dependent expression of cytochrome c oxidase subunit ... | ACCEPT | Summary: Sequence-specific DNA binding (e.g. the COX4I2 oxygen responsive element). Reason: Consistent with the core DNA-binding function. Supporting Evidence: PMID:23303788 Here, we have identified three transcription factors that bind this conserved ORE, namely recombination signal sequence-binding protein JΞΊ (RBPJ), coiled-coil-helix-coiled-coil-helix domain 2 (CHCHD2) and CXXC finger protein 5 (CXXC5). file:human/RBPJ/RBPJ-uniprot.txt Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. |
| GO:0043565 sequence-specific DNA binding | IEA GO_REF:0000107 | ACCEPT | Summary: Sequence-specific DNA binding (e.g. the COX4I2 oxygen responsive element). Reason: Consistent with the core DNA-binding function. Supporting Evidence: PMID:23303788 Here, we have identified three transcription factors that bind this conserved ORE, namely recombination signal sequence-binding protein JΞΊ (RBPJ), coiled-coil-helix-coiled-coil-helix domain 2 (CHCHD2) and CXXC finger protein 5 (CXXC5). file:human/RBPJ/RBPJ-uniprot.txt Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. |
| GO:0045892 negative regulation of DNA-templated transcription | IDA PMID:10713164 SKIP, a CBF1-associated protein, interacts with the ankyrin ... | ACCEPT | Summary: RBPJ represses Notch targets in the absence of NICD by recruiting corepressors. Reason: Core repressor activity of CSL proteins. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Acts as a transcriptional repressor when it is not associated with Notch proteins. PMID:10713164 CBF1 mediates transcriptional repression through the recruitment of an SMRT-histone deacetylase-containing corepressor complex. |
| GO:0045893 positive regulation of DNA-templated transcription | IDA PMID:32601208 Pharmacological disruption of the Notch transcription factor... | ACCEPT | Summary: RBPJ activates transcription with NICD/MAML (and at the COX4I2 ORE). Reason: Core activator activity. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. PMID:23303788 We demonstrate that RBPJ and CHCHD2 function towards activating the ORE at 4% oxygen, whereas CXXC5 functions as an inhibitor. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IDA PMID:23303788 Oxygen-dependent expression of cytochrome c oxidase subunit ... | ACCEPT | Summary: RBPJ activates transcription with NICD/MAML (and at the COX4I2 ORE). Reason: Core activator activity. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. PMID:23303788 We demonstrate that RBPJ and CHCHD2 function towards activating the ORE at 4% oxygen, whereas CXXC5 functions as an inhibitor. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IEA GO_REF:0000107 | ACCEPT | Summary: RBPJ activates transcription with NICD/MAML (and at the COX4I2 ORE). Reason: Core activator activity. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. PMID:23303788 We demonstrate that RBPJ and CHCHD2 function towards activating the ORE at 4% oxygen, whereas CXXC5 functions as an inhibitor. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IMP PMID:18663143 Slug is a direct Notch target required for initiation of car... | ACCEPT | Summary: Notch-induced activation of target genes requires CSL/RBPJ. Reason: CSL knockdown abolishes Notch-induced target induction. Supporting Evidence: PMID:18663143 Dll4-mediated induction of Slug mRNA and protein was dramatically reduced when CSL was knocked down using either of two lentiviral-delivered short hairpin RNA (shRNA) constructs |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IMP PMID:20890042 Integration of a Notch-dependent mesenchymal gene program an... | ACCEPT | Summary: Notch-induced activation of target genes requires CSL/RBPJ. Reason: CSL knockdown abolishes Notch-induced target induction. Supporting Evidence: PMID:18663143 Dll4-mediated induction of Slug mRNA and protein was dramatically reduced when CSL was knocked down using either of two lentiviral-delivered short hairpin RNA (shRNA) constructs |
| GO:0045944 positive regulation of transcription by RNA polymerase II | ISS GO_REF:0000024 | ACCEPT | Summary: RBPJ activates transcription with NICD/MAML (and at the COX4I2 ORE). Reason: Core activator activity. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. PMID:23303788 We demonstrate that RBPJ and CHCHD2 function towards activating the ORE at 4% oxygen, whereas CXXC5 functions as an inhibitor. |
| GO:0048513 animal organ development | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Animal organ development (ARBA). Reason: Broad; non-core. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0060045 positive regulation of cardiac muscle cell proliferation | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0060045 positive regulation of cardiac muscle cell proliferation | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0060379 cardiac muscle cell myoblast differentiation | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0060379 cardiac muscle cell myoblast differentiation | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0060412 ventricular septum morphogenesis | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0060716 labyrinthine layer blood vessel development | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Vascular development roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0060716 labyrinthine layer blood vessel development | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Vascular development roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0060844 arterial endothelial cell fate commitment | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Vascular development roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0061344 regulation of cell adhesion involved in heart morphogenesis | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding | IPI PMID:16287852 RBP-Jkappa/SHARP recruits CtIP/CtBP corepressors to silence ... | MODIFY | Summary: RBPJ binds SHARP/SPEN, which recruits CtIP/CtBP corepressors. Reason: SPEN is a corepressor rather than a DNA-binding transcription factor, so transcription corepressor binding is the correct MF. Proposed replacements: transcription corepressor binding Supporting Evidence: PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. PMID:16287852 We and others have identified SHARP as a component of this complex. |
| GO:0072554 blood vessel lumenization | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Vascular development roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0072554 blood vessel lumenization | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Vascular development roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0097101 blood vessel endothelial cell fate specification | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Vascular development roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0097101 blood vessel endothelial cell fate specification | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Vascular development roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: file:human/RBPJ/RBPJ-uniprot.txt Transcriptional regulator that plays a central role in Notch signaling |
| GO:0120163 negative regulation of cold-induced thermogenesis | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Adipose Rbpj deletion causes WAT browning in mice. Reason: Rbpj-mediated Notch transcription represses thermogenic genes in adipocytes; physiological, non-core. Supporting Evidence: PMID:25038826 adipose-specific inactivation of Notch1 or its signaling mediator Rbpj in mice results in browning of WAT and elevated expression of uncoupling protein 1 (Ucp1) |
| GO:0120163 negative regulation of cold-induced thermogenesis | ISS PMID:25038826 Inhibition of Notch signaling promotes browning of white adi... | KEEP AS NON CORE | Summary: Adipose Rbpj deletion causes WAT browning in mice. Reason: Rbpj-mediated Notch transcription represses thermogenic genes in adipocytes; physiological, non-core. Supporting Evidence: PMID:25038826 adipose-specific inactivation of Notch1 or its signaling mediator Rbpj in mice results in browning of WAT and elevated expression of uncoupling protein 1 (Ucp1) |
| GO:0140297 DNA-binding transcription factor binding | IDA PMID:23303788 Oxygen-dependent expression of cytochrome c oxidase subunit ... | KEEP AS NON CORE | Summary: RBPJ co-occupies the COX4I2 oxygen responsive element with CHCHD2 and CXXC5. Reason: Reported co-binding of DNA-binding factors at one promoter; non-core. Supporting Evidence: PMID:23303788 Here, we have identified three transcription factors that bind this conserved ORE, namely recombination signal sequence-binding protein JΞΊ (RBPJ), coiled-coil-helix-coiled-coil-helix domain 2 (CHCHD2) and CXXC finger protein 5 (CXXC5). |
| GO:0140297 DNA-binding transcription factor binding | IPI PMID:16691198 A metabolic enzyme of the short-chain dehydrogenase/reductas... | KEEP AS NON CORE | Summary: Interaction with the retinol dehydrogenase hRoDH-E2 (SDR16C5). Reason: The partner is a metabolic enzyme proposed to act as a corepressor, not a classical DNA-binding transcription factor; retained as non-core, with transcription corepressor binding being a better description of this partner. Supporting Evidence: PMID:16691198 This study provides evidence that an enzyme, hRoDH-E2, with cytoplasmic phosphorylated and reduced forms of NAD-dependent retinol dehydrogenase activity may function in the nucleus as a transcriptional repressor. |
| GO:1901186 positive regulation of ERBB signaling pathway | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Positive regulation of BMP/ERBB/ephrin signaling (mouse Rbpj). Reason: Cross-pathway effects of Notch target gene expression in cardiac development; indirect for RBPJ. Supporting Evidence: PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:1901186 positive regulation of ERBB signaling pathway | ISS GO_REF:0000024 | MARK AS OVER ANNOTATED | Summary: Positive regulation of BMP/ERBB/ephrin signaling (mouse Rbpj). Reason: Cross-pathway effects of Notch target gene expression in cardiac development; indirect for RBPJ. Supporting Evidence: PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:1901189 positive regulation of ephrin receptor signaling pathway | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Positive regulation of BMP/ERBB/ephrin signaling (mouse Rbpj). Reason: Cross-pathway effects of Notch target gene expression in cardiac development; indirect for RBPJ. Supporting Evidence: PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:1901189 positive regulation of ephrin receptor signaling pathway | ISS GO_REF:0000024 | MARK AS OVER ANNOTATED | Summary: Positive regulation of BMP/ERBB/ephrin signaling (mouse Rbpj). Reason: Cross-pathway effects of Notch target gene expression in cardiac development; indirect for RBPJ. Supporting Evidence: PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:1990433 CSL-Notch-Mastermind transcription factor complex | IPI PMID:19907488 Direct inhibition of the NOTCH transcription factor complex. | ACCEPT | Summary: RBPJ is the DNA-binding core of the CSL-NICD-MAML ternary activation complex. Reason: Structurally and biochemically defined core complex (PTN002580211 for the IBA). Supporting Evidence: PMID:16530044 We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). PMID:19907488 We demonstrate that direct, high-affinity binding of the hydrocarbon-stapled peptide SAHM1 prevents assembly of the active transcriptional complex. PMID:16510869 MAML1's promyogenic effects were completely blocked upon activation of Notch signaling, which was associated with recruitment of MAML1 away from MEF2C to the Notch transcriptional complex. |
| GO:2000138 positive regulation of cell proliferation involved in heart morphogenesis | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
| GO:2000138 positive regulation of cell proliferation involved in heart morphogenesis | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Cardiac morphogenesis/EMT roles (mouse Rbpj). Reason: Developmental role transferred from mouse Rbpj. RBPJ executes this process only as the transcription factor of Notch signaling in the relevant cells; it is a real but downstream, tissue-specific outcome, retained as non-core. Supporting Evidence: PMID:18663143 We further demonstrate that Slug, but not Snail, is directly up-regulated by Notch in endothelial cells and that Slug expression is required for Notch-mediated repression of the vascular endothelial cadherin promoter PMID:20890042 Notch deletion abrogates endocardial Hey gene transcription and extends Bmp2 expression to the ventricular endocardium. |
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Download this section (compressed HTML)Q: Which RBPJ-bound enhancers are repressed at steady state in human cells, and how much of Notch target activation reflects de-repression versus active recruitment?
Q: Is RBPJ's activation of COX4I2 under hypoxia Notch-dependent?
Experiment: Map RBPJ occupancy and corepressor/coactivator exchange (SPEN, L3MBTL3, MAML1) by CUT&RUN before and after gamma-secretase inhibitor washout in human T-ALL cells.
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