RPL36A encodes the 106-residue large ribosomal subunit protein eL42, historically called 60S ribosomal protein L36a or L44. It is a conserved structural constituent of the human cytosolic 60S subunit and participates in cytoplasmic translation. Human 80S and pre-60S cryo-EM structures place eL42 in assembled and maturing large subunits. Its primary localization is cytosolic, with endoplasmic-reticulum signal plausibly arising from translating ribosomes bound to rough ER; generic interaction and RNA-capture records do not establish independent extraribosomal functions.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0002181 cytoplasmic translation | IBA GO_REF:0000033 | ACCEPT | Summary: RPL36A/eL42 is a constitutive component of the cytosolic 60S ribosomal subunit and supports cytoplasmic protein synthesis. Reason: Reviewed UniProt and multiple human 80S/pre-60S structures directly establish large-subunit membership; the annotated process and molecular function describe the protein’s conserved core role. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0003735 structural constituent of ribosome | IBA GO_REF:0000033 | ACCEPT | Summary: RPL36A/eL42 is a constitutive component of the cytosolic 60S ribosomal subunit and supports cytoplasmic protein synthesis. Reason: Reviewed UniProt and multiple human 80S/pre-60S structures directly establish large-subunit membership; the annotated process and molecular function describe the protein’s conserved core role. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0022625 cytosolic large ribosomal subunit | IBA GO_REF:0000033 | ACCEPT | Summary: RPL36A/eL42 is a constitutive component of the cytosolic 60S ribosomal subunit and supports cytoplasmic protein synthesis. Reason: Reviewed UniProt and multiple human 80S/pre-60S structures directly establish large-subunit membership; the annotated process and molecular function describe the protein’s conserved core role. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0003735 structural constituent of ribosome | IEA GO_REF:0000002 | ACCEPT | Summary: RPL36A/eL42 is a constitutive component of the cytosolic 60S ribosomal subunit and supports cytoplasmic protein synthesis. Reason: Reviewed UniProt and multiple human 80S/pre-60S structures directly establish large-subunit membership; the annotated process and molecular function describe the protein’s conserved core role. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005840 ribosome | IEA GO_REF:0000002 | MODIFY | Summary: RPL36A is located in ribosomes, specifically in the cytosolic 60S subunit. Reason: The broad ribosome term is correct but less informative than the experimentally supported cytosolic large ribosomal subunit term. Proposed replacements: cytosolic large ribosomal subunit Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. |
| GO:0006412 translation | IEA GO_REF:0000002 | MODIFY | Summary: RPL36A participates in translation as a component of the cytosolic large ribosomal subunit. Reason: Cytoplasmic translation is the more precise process for a human cytosolic-ribosome constituent and is independently supported by multiple existing annotations. Proposed replacements: cytoplasmic translation Supporting Evidence: file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: A proteome-scale binary-interaction screen reports an RPL36A interaction. Reason: The high-throughput interaction can be retained as an observation, but it does not establish a partner-specific biochemical activity and generic protein binding is not informative. Supporting Evidence: PMID:32296183 A reference map of the human binary protein interactome. |
| GO:0002181 cytoplasmic translation | NAS PMID:25901680 Structure of the human 80S ribosome. | ACCEPT | Summary: RPL36A/eL42 is a constitutive component of the cytosolic 60S ribosomal subunit and supports cytoplasmic protein synthesis. Reason: Reviewed UniProt and multiple human 80S/pre-60S structures directly establish large-subunit membership; the annotated process and molecular function describe the protein’s conserved core role. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0005737 cytoplasm | NAS PMID:25901680 Structure of the human 80S ribosome. | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0006941 striated muscle contraction | NAS PMID:34081545 Knockdown of Muscle-Specific Ribosomal Protein L3-Like Enhan... | MARK AS OVER ANNOTATED | Summary: ComplexPortal propagated striated-muscle contraction from a study that perturbs muscle-specific RPL3L. Reason: The cited mouse study concerns Rpl3l, not RPL36A/eL42. Membership of RPL36A in a muscle ribosome does not establish a specific role in striated-muscle contraction for this constitutive subunit. Supporting Evidence: PMID:34081545 Muscle-Specific Ribosomal Protein L3-Like |
| GO:1901740 negative regulation of myoblast fusion | NAS PMID:26684695 Expression of Muscle-Specific Ribosomal Protein L3-Like Impa... | MARK AS OVER ANNOTATED | Summary: ComplexPortal propagated a myoblast-fusion phenotype from ectopic expression of muscle-specific RPL3L. Reason: The full text perturbs RPL3L and does not assay RPL36A. Extending the phenotype to every constituent of the resulting ribosome is an over-annotation rather than RPL36A-specific evidence. Supporting Evidence: PMID:26684695 RPL3L expression significantly impaired myotube growth |
| GO:0005783 endoplasmic reticulum | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: HPA detects an endoplasmic-reticulum-associated pool of RPL36A. Reason: The localization is plausible for cytosolic ribosomes engaged on rough ER, but it does not establish stable ER residency or a distinct ER-specific function of RPL36A. Supporting Evidence: file:human/RPL36A/RPL36A-notes.md it can reflect rough-ER-bound cytosolic ribosomes. |
| GO:0005829 cytosol | IDA GO_REF:0000052 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0022626 cytosolic ribosome | IDA PMID:23636399 Structures of the human and Drosophila 80S ribosome. | ACCEPT | Summary: RPL36A/eL42 is a constitutive component of the cytosolic 60S ribosomal subunit and supports cytoplasmic protein synthesis. Reason: Reviewed UniProt and multiple human 80S/pre-60S structures directly establish large-subunit membership; the annotated process and molecular function describe the protein’s conserved core role. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0005737 cytoplasm | EXP PMID:23636399 Structures of the human and Drosophila 80S ribosome. | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0003735 structural constituent of ribosome | IDA PMID:32669547 Structural snapshots of human pre-60S ribosomal particles be... | ACCEPT | Summary: RPL36A/eL42 is a constitutive component of the cytosolic 60S ribosomal subunit and supports cytoplasmic protein synthesis. Reason: Reviewed UniProt and multiple human 80S/pre-60S structures directly establish large-subunit membership; the annotated process and molecular function describe the protein’s conserved core role. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0022625 cytosolic large ribosomal subunit | IDA PMID:32669547 Structural snapshots of human pre-60S ribosomal particles be... | ACCEPT | Summary: RPL36A/eL42 is a constitutive component of the cytosolic 60S ribosomal subunit and supports cytoplasmic protein synthesis. Reason: Reviewed UniProt and multiple human 80S/pre-60S structures directly establish large-subunit membership; the annotated process and molecular function describe the protein’s conserved core role. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0002181 cytoplasmic translation | IC PMID:23636399 Structures of the human and Drosophila 80S ribosome. | ACCEPT | Summary: RPL36A/eL42 is a constitutive component of the cytosolic 60S ribosomal subunit and supports cytoplasmic protein synthesis. Reason: Reviewed UniProt and multiple human 80S/pre-60S structures directly establish large-subunit membership; the annotated process and molecular function describe the protein’s conserved core role. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0003735 structural constituent of ribosome | IDA PMID:23636399 Structures of the human and Drosophila 80S ribosome. | ACCEPT | Summary: RPL36A/eL42 is a constitutive component of the cytosolic 60S ribosomal subunit and supports cytoplasmic protein synthesis. Reason: Reviewed UniProt and multiple human 80S/pre-60S structures directly establish large-subunit membership; the annotated process and molecular function describe the protein’s conserved core role. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0002181 cytoplasmic translation | IDA PMID:25957688 Structural snapshots of actively translating human ribosomes... | ACCEPT | Summary: RPL36A/eL42 is a constitutive component of the cytosolic 60S ribosomal subunit and supports cytoplasmic protein synthesis. Reason: Reviewed UniProt and multiple human 80S/pre-60S structures directly establish large-subunit membership; the annotated process and molecular function describe the protein’s conserved core role. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0022625 cytosolic large ribosomal subunit | IDA PMID:25957688 Structural snapshots of actively translating human ribosomes... | ACCEPT | Summary: RPL36A/eL42 is a constitutive component of the cytosolic 60S ribosomal subunit and supports cytoplasmic protein synthesis. Reason: Reviewed UniProt and multiple human 80S/pre-60S structures directly establish large-subunit membership; the annotated process and molecular function describe the protein’s conserved core role. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0005515 protein binding | IPI PMID:24965446 Host factors that interact with the pestivirus N-terminal pr... | MARK AS OVER ANNOTATED | Summary: A viral Npro pulldown recovered RPL36A together with many ribosomal and ribonucleoprotein components. Reason: The study explicitly allows indirect co-purification through a larger complex. This interaction observation does not define an independent RPL36A molecular function, and generic protein binding is uninformative. Supporting Evidence: PMID:24965446 It may be that Npro interacts with one or two of these RNA binding proteins, and the other proteins are pulled down in the same complex. |
| GO:0002181 cytoplasmic translation | IC PMID:12962325 Characterization and analysis of posttranslational modificat... | ACCEPT | Summary: RPL36A/eL42 is a constitutive component of the cytosolic 60S ribosomal subunit and supports cytoplasmic protein synthesis. Reason: Reviewed UniProt and multiple human 80S/pre-60S structures directly establish large-subunit membership; the annotated process and molecular function describe the protein’s conserved core role. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0003735 structural constituent of ribosome | IC PMID:12962325 Characterization and analysis of posttranslational modificat... | ACCEPT | Summary: RPL36A/eL42 is a constitutive component of the cytosolic 60S ribosomal subunit and supports cytoplasmic protein synthesis. Reason: Reviewed UniProt and multiple human 80S/pre-60S structures directly establish large-subunit membership; the annotated process and molecular function describe the protein’s conserved core role. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0003723 RNA binding | HDA PMID:22681889 The mRNA-bound proteome and its global occupancy profile on ... | MARK AS OVER ANNOTATED | Summary: An mRNA interactome captured RPL36A in the polyadenylated-RNA-associated proteome. Reason: For a constitutive ribosomal protein, whole-cell mRNA capture does not distinguish direct autonomous RNA binding from recovery of an assembled translating ribosome. Structural-constituent activity captures the supported core function. Supporting Evidence: PMID:22681889 The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-156826 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1799332 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-1799335 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-192704 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-192841 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-6791218 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-72671 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-72672 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-72673 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-927789 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-927813 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-927832 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-927836 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-927889 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9948291 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9948300 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9948301 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9948317 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9948318 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9948360 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9948362 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9948427 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9948458 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9954723 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9954727 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9954730 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9954919 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0005829 cytosol | TAS Reactome:R-HSA-9955731 | ACCEPT | Summary: RPL36A is a cytoplasmic/cytosolic ribosomal protein. Reason: Human ribosome structures and the reviewed UniProt localization support cytoplasmic placement; Reactome consistently represents the cytosolic ribosome pool. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Cytoplasm {ECO:0000269|PubMed:23636399}. |
| GO:0003735 structural constituent of ribosome | NAS PMID:12962325 Characterization and analysis of posttranslational modificat... | ACCEPT | Summary: RPL36A/eL42 is a constitutive component of the cytosolic 60S ribosomal subunit and supports cytoplasmic protein synthesis. Reason: Reviewed UniProt and multiple human 80S/pre-60S structures directly establish large-subunit membership; the annotated process and molecular function describe the protein’s conserved core role. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0006412 translation | NAS PMID:12962325 Characterization and analysis of posttranslational modificat... | MODIFY | Summary: RPL36A participates in translation as a component of the cytosolic large ribosomal subunit. Reason: Cytoplasmic translation is the more precise process for a human cytosolic-ribosome constituent and is independently supported by multiple existing annotations. Proposed replacements: cytoplasmic translation Supporting Evidence: file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0022625 cytosolic large ribosomal subunit | HDA PMID:12962325 Characterization and analysis of posttranslational modificat... | ACCEPT | Summary: RPL36A/eL42 is a constitutive component of the cytosolic 60S ribosomal subunit and supports cytoplasmic protein synthesis. Reason: Reviewed UniProt and multiple human 80S/pre-60S structures directly establish large-subunit membership; the annotated process and molecular function describe the protein’s conserved core role. Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. file:human/RPL36A/RPL36A-deep-research-manual.md Evidence conclusion: RPL36A/eL42 is a structural component of the cytosolic 60S |
| GO:0005840 ribosome | NAS PMID:3542712 Characterisation of an mRNA encoding a human ribosomal prote... | MODIFY | Summary: RPL36A is located in ribosomes, specifically in the cytosolic 60S subunit. Reason: The broad ribosome term is correct but less informative than the experimentally supported cytosolic large ribosomal subunit term. Proposed replacements: cytosolic large ribosomal subunit Supporting Evidence: file:human/RPL36A/RPL36A-uniprot.txt Component of the large ribosomal subunit. |
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Download this section (compressed HTML)Q: What fraction of native human eL42 is supplied by RPL36A versus RPL36AL in each tissue?
Q: Does RPL36A have any reproducible extraribosomal function after controlling for whole-ribosome co-purification?
Q: Is the apparent ER pool entirely explained by rough-ER-bound polysomes?
Experiment: Develop paralog-specific targeted peptides and quantify each protein in affinity-purified ribosomes across primary human tissues, coupled to single-gene CRISPR perturbation and rescue.
Hypothesis: RPL36A and RPL36AL contribute unequally to eL42 occupancy in a tissue-dependent manner.
Type: Paralog-resolved ribosome proteomics
Experiment: Compare endogenous RPL36A imaging and membrane fractionation before and after puromycin-induced polysome runoff, translation arrest, and SRP-pathway perturbation.
Hypothesis: The RPL36A ER signal depends on active cotranslational targeting rather than stable ER residence.
Type: Translation-dependent localization analysis
Experiment: Map endogenous RPL36A RNA contacts by stringent UV crosslinking and nucleotide-resolution CLIP, then compare contact profiles after ribosome-disassembly conditions and with assembled-ribosome controls.
Hypothesis: mRNA interactome recovery reflects assembled-ribosome capture rather than a separate RNA-binding function.
Type: Ribosome-aware RNA crosslink mapping
What is not known — curated, literature-grounded statements of the open unknowns (the inverse of core functions).
Gap: The relative incorporation and tissue distribution of RPL36A versus its close paralog RPL36AL in native human ribosomes are unresolved.
OPEN BIOLOGY BP_DARK
What is known: Current human ribosome structures establish eL42 occupancy but do not generally distinguish which near-identical gene product supplies each observed chain.
Significance: Paralog-resolved occupancy is necessary before assigning tissue-specific or specialized-ribosome functions to either gene.
What would resolve it: Use paralog-specific peptides, targeted proteomics, and ribosome profiling after single-gene perturbation across human tissues and cell states.
Provenance (the field's own admissions):
Gap: The HPA endoplasmic-reticulum signal has not been separated from localization of rough-ER-bound translating ribosomes.
OPEN BIOLOGY CC_DARK
What is known: The current annotation reports ER association, but no experiment in the reviewed set shows an ER-resident RPL36A pool independent of cytosolic ribosomes.
Significance: Resolving this prevents a transient translation-site association from being mistaken for a distinct organelle function.
What would resolve it: Measure RPL36A localization after polysome runoff and disruption of SRP-dependent ER targeting, with biochemical fractionation and quantitative imaging.
Provenance (the field's own admissions):
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