id: Q9Y371
gene_symbol: SH3GLB1
product_type: PROTEIN
status: COMPLETE
taxon:
  id: NCBITaxon:9606
  label: Homo sapiens
description: >-
  SH3GLB1 encodes endophilin B1/Bif-1, an N-BAR/SH3 membrane-curvature adaptor that links
  UVRAG/BECN1/PI3KC3-C2 autophagy signaling to ATG9-positive Golgi membrane fission and autophagosome
  assembly. Its core functions include PI3KC3 activation, adaptor-mediated association with
  UVRAG/Beclin 1, and membrane fission/tubulation at Golgi/autophagosome membranes. SH3GLB1 also has
  supported non-core roles in mitochondrial outer membrane dynamics, Bax/Bak-dependent apoptosis,
  receptor degradation, cytokinesis, and older lipid-binding/acyltransferase biochemistry.
alternative_products:
- name: '1'
  id: Q9Y371-1
- name: '2'
  id: Q9Y371-2
  sequence_note: VSP_009276
- name: '3'
  id: Q9Y371-3
  sequence_note: VSP_044895
existing_annotations:
- term:
    id: GO:0006897
    label: endocytosis
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  review:
    summary: Endocytosis is directionally related but too broad for SH3GLB1.
    action: MODIFY
    reason: SH3GLB1/endophilin B1 contributes to membrane curvature, ATG9/Golgi fission, receptor degradation,
      and endocytic trafficking contexts; the broad endocytosis parent should be narrowed where possible.
    proposed_replacement_terms:
    - id: GO:0090148
      label: membrane fission
    - id: GO:0032801
      label: receptor catabolic process
    additional_reference_ids:
    - PMID:20643123
    - PMID:21068542
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:21068542
      supporting_text: tubulation and fragmentation of Atg9-positive Golgi membranes
    - reference_id: PMID:21068542
      supporting_text: Bif-1/Endophilin B1 and the PI3KC3 complex II
    - reference_id: PMID:20643123
      supporting_text: regulates both receptor degradation and cytokinesis
- term:
    id: GO:0016020
    label: membrane
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: is_active_in
  review:
    summary: Membrane is true but too broad for a peripheral N-BAR autophagy/mitochondrial membrane factor.
    action: MODIFY
    reason: 'Use the supported specific membrane locations: Golgi membrane, autophagosome membrane, and mitochondrial
      outer membrane, with the mitochondrial branch retained as non-core.'
    proposed_replacement_terms:
    - id: GO:0000139
      label: Golgi membrane
    - id: GO:0000421
      label: autophagosome membrane
    - id: GO:0005741
      label: mitochondrial outer membrane
    additional_reference_ids:
    - PMID:17891140
    - PMID:21068542
    - PMID:15452144
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Golgi apparatus membrane
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Mitochondrion outer membrane
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Cytoplasmic vesicle, autophagosome
- term:
    id: GO:0030674
    label: protein-macromolecule adaptor activity
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: enables
  review:
    summary: Protein-macromolecule adaptor activity is a good core molecular-function description for Bif-1.
    action: ACCEPT
    reason: SH3GLB1 uses BAR/SH3-mediated membrane and protein interactions to connect UVRAG/BECN1/PI3KC3-C2 
      with autophagy membrane remodeling.
    additional_reference_ids:
    - PMID:17891140
    - PMID:20643123
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:17891140
      supporting_text: interacts with Beclin 1 through ultraviolet irradiation resistance-associated gene 
        (UVRAG)
    - reference_id: PMID:20643123
      supporting_text: specific sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and BIF-1
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG; UVRAG bridges the interaction to BECN1
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: complex II (PI3KC3-C2)
    - reference_id: PMID:11604418
      supporting_text: directly bind and evaginate lipid bilayers into narrow tubules
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: induce membrane curvature and bind curved membranes
- term:
    id: GO:0061024
    label: membrane organization
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  review:
    summary: Membrane organization is supported but should be represented by the more specific 
      fission/tubulation terms.
    action: MODIFY
    reason: Bif-1/endophilin B1 is an N-BAR membrane-curvature protein; the specific supported processes are 
      membrane fission and positive regulation of membrane tubulation during ATG9/Golgi trafficking.
    proposed_replacement_terms:
    - id: GO:0090148
      label: membrane fission
    - id: GO:1903527
      label: positive regulation of membrane tubulation
    additional_reference_ids:
    - PMID:11604418
    - PMID:21068542
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:11604418
      supporting_text: directly bind and evaginate lipid bilayers into narrow tubules
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: induce membrane curvature and bind curved membranes
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: N-BAR domain
    - reference_id: PMID:21068542
      supporting_text: tubulation and fragmentation of Atg9-positive Golgi membranes
    - reference_id: PMID:21068542
      supporting_text: Bif-1/Endophilin B1 and the PI3KC3 complex II
- term:
    id: GO:0000139
    label: Golgi membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  review:
    summary: Golgi membrane localization is core for Bif-1-mediated ATG9 trafficking.
    action: ACCEPT
    reason: SH3GLB1 localizes to Golgi membranes and drives starvation-induced Atg9-positive Golgi membrane 
      fission for autophagosome biogenesis.
    additional_reference_ids:
    - PMID:21068542
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:21068542
      supporting_text: tubulation and fragmentation of Atg9-positive Golgi membranes
    - reference_id: PMID:21068542
      supporting_text: Bif-1/Endophilin B1 and the PI3KC3 complex II
    - reference_id: PMID:21068542
      supporting_text: the fission of Golgi membranes and the trafficking of Atg9 from the Golgi complex to 
        autophagosomes
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: regulate the trafficking of ATG9A from the Golgi complex
- term:
    id: GO:0000421
    label: autophagosome membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  review:
    summary: Autophagosome membrane localization is core for SH3GLB1/Bif-1 autophagy function.
    action: ACCEPT
    reason: Bif-1 localizes to autophagosomes after nutrient deprivation and supports autophagosome formation 
      through UVRAG/PI3KC3-C2.
    additional_reference_ids:
    - PMID:17891140
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:17891140
      supporting_text: localizes to autophagosomes
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Cytoplasmic vesicle, autophagosome
    - reference_id: PMID:17891140
      supporting_text: functions as a positive mediator of the class III PI(3) kinase (PI(3)KC3)
    - reference_id: PMID:17891140
      supporting_text: loss of Bif-1 suppresses autophagosome formation
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  qualifier: located_in
  review:
    summary: Cytoplasmic/cytosolic localization is broad but compatible with SH3GLB1.
    action: KEEP_AS_NON_CORE
    reason: More specific membrane locations drive the functional review; cytoplasm/cytosol is retained as 
      broad context only.
    proposed_replacement_terms:
    - id: GO:0000139
      label: Golgi membrane
    - id: GO:0000421
      label: autophagosome membrane
    - id: GO:0005741
      label: mitochondrial outer membrane
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Cytoplasm {ECO:0000269|PubMed:15452144}
- term:
    id: GO:0005741
    label: mitochondrial outer membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  review:
    summary: Mitochondrial outer membrane localization is supported but non-core for this PN review.
    action: KEEP_AS_NON_CORE
    reason: SH3GLB1 has a real mitochondrial morphology/apoptosis branch, but the PN-relevant core is 
      PI3KC3-C2/autophagosome assembly.
    additional_reference_ids:
    - PMID:15452144
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:15452144
      supporting_text: required for maintenance of mitochondrial morphology
    - reference_id: PMID:15452144
      supporting_text: required for the regulation of the outer mitochondrial membrane dynamics
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: May be required for normal outer mitochondrial membrane dynamics
- term:
    id: GO:0030496
    label: midbody
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  review:
    summary: Midbody localization is supported for the PI3KC3-C2/BIF-1 cytokinesis branch but non-core.
    action: KEEP_AS_NON_CORE
    reason: The cytokinesis/midbody role is real but separate from the autophagy/ATG9 membrane fission core.
    additional_reference_ids:
    - PMID:20643123
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:20643123
      supporting_text: regulates both receptor degradation and cytokinesis
    - reference_id: PMID:20643123
      supporting_text: strong localisation of these proteins to the midbody
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:17891140
  qualifier: enables
  review:
    summary: The interaction evidence may be real, but generic protein binding is not an informative SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: Replace generic protein binding with specific adaptor activity, PI3KC3-C2 association, 
      homodimerization, membrane curvature/fission, or Bax/mitochondrial apoptosis context as appropriate.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:20562859
  qualifier: enables
  review:
    summary: The interaction evidence may be real, but generic protein binding is not an informative SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: Replace generic protein binding with specific adaptor activity, PI3KC3-C2 association, 
      homodimerization, membrane curvature/fission, or Bax/mitochondrial apoptosis context as appropriate.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:21988832
  qualifier: enables
  review:
    summary: The interaction evidence may be real, but generic protein binding is not an informative SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: Replace generic protein binding with specific adaptor activity, PI3KC3-C2 association, 
      homodimerization, membrane curvature/fission, or Bax/mitochondrial apoptosis context as appropriate.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:23414517
  qualifier: enables
  review:
    summary: The interaction evidence may be real, but generic protein binding is not an informative SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: Replace generic protein binding with specific adaptor activity, PI3KC3-C2 association, 
      homodimerization, membrane curvature/fission, or Bax/mitochondrial apoptosis context as appropriate.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:24056303
  qualifier: enables
  review:
    summary: The interaction evidence may be real, but generic protein binding is not an informative SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: Replace generic protein binding with specific adaptor activity, PI3KC3-C2 association, 
      homodimerization, membrane curvature/fission, or Bax/mitochondrial apoptosis context as appropriate.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:25416956
  qualifier: enables
  review:
    summary: The interaction evidence may be real, but generic protein binding is not an informative SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: Replace generic protein binding with specific adaptor activity, PI3KC3-C2 association, 
      homodimerization, membrane curvature/fission, or Bax/mitochondrial apoptosis context as appropriate.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:25910212
  qualifier: enables
  review:
    summary: The interaction evidence may be real, but generic protein binding is not an informative SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: Replace generic protein binding with specific adaptor activity, PI3KC3-C2 association, 
      homodimerization, membrane curvature/fission, or Bax/mitochondrial apoptosis context as appropriate.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:27107012
  qualifier: enables
  review:
    summary: The interaction evidence may be real, but generic protein binding is not an informative SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: Replace generic protein binding with specific adaptor activity, PI3KC3-C2 association, 
      homodimerization, membrane curvature/fission, or Bax/mitochondrial apoptosis context as appropriate.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:31515488
  qualifier: enables
  review:
    summary: The interaction evidence may be real, but generic protein binding is not an informative SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: Replace generic protein binding with specific adaptor activity, PI3KC3-C2 association, 
      homodimerization, membrane curvature/fission, or Bax/mitochondrial apoptosis context as appropriate.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:32296183
  qualifier: enables
  review:
    summary: The interaction evidence may be real, but generic protein binding is not an informative SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: Replace generic protein binding with specific adaptor activity, PI3KC3-C2 association, 
      homodimerization, membrane curvature/fission, or Bax/mitochondrial apoptosis context as appropriate.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:32814053
  qualifier: enables
  review:
    summary: The interaction evidence may be real, but generic protein binding is not an informative SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: Replace generic protein binding with specific adaptor activity, PI3KC3-C2 association, 
      homodimerization, membrane curvature/fission, or Bax/mitochondrial apoptosis context as appropriate.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:33961781
  qualifier: enables
  review:
    summary: The interaction evidence may be real, but generic protein binding is not an informative SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: Replace generic protein binding with specific adaptor activity, PI3KC3-C2 association, 
      homodimerization, membrane curvature/fission, or Bax/mitochondrial apoptosis context as appropriate.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:35271311
  qualifier: enables
  review:
    summary: The interaction evidence may be real, but generic protein binding is not an informative SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: Replace generic protein binding with specific adaptor activity, PI3KC3-C2 association, 
      homodimerization, membrane curvature/fission, or Bax/mitochondrial apoptosis context as appropriate.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:39526800
  qualifier: enables
  review:
    summary: The interaction evidence may be real, but generic protein binding is not an informative SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: Replace generic protein binding with specific adaptor activity, PI3KC3-C2 association, 
      homodimerization, membrane curvature/fission, or Bax/mitochondrial apoptosis context as appropriate.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:40205054
  qualifier: enables
  review:
    summary: The interaction evidence may be real, but generic protein binding is not an informative SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: Replace generic protein binding with specific adaptor activity, PI3KC3-C2 association, 
      homodimerization, membrane curvature/fission, or Bax/mitochondrial apoptosis context as appropriate.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1
- term:
    id: GO:0042802
    label: identical protein binding
  evidence_type: IPI
  original_reference_id: PMID:20562859
  qualifier: enables
  review:
    summary: Identical protein binding is too generic for SH3GLB1 oligomerization biology.
    action: MODIFY
    reason: SH3GLB1 forms homodimers/oligomers as part of endophilin membrane-curvature function; the 
      homodimerization term is more specific.
    proposed_replacement_terms:
    - id: GO:0042803
      label: protein homodimerization activity
    additional_reference_ids:
    - PMID:11161816
    - PMID:19805544
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:11161816
      supporting_text: SH3GLB, a new endophilin-related protein family featuring an SH3 domain
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Homodimer, and heterodimer with SH3GLB2
    - reference_id: PMID:19805544
      supporting_text: Endo B1 oligomerization is induced by Bax
- term:
    id: GO:0042802
    label: identical protein binding
  evidence_type: IPI
  original_reference_id: PMID:23414517
  qualifier: enables
  review:
    summary: Identical protein binding is too generic for SH3GLB1 oligomerization biology.
    action: MODIFY
    reason: SH3GLB1 forms homodimers/oligomers as part of endophilin membrane-curvature function; the 
      homodimerization term is more specific.
    proposed_replacement_terms:
    - id: GO:0042803
      label: protein homodimerization activity
    additional_reference_ids:
    - PMID:11161816
    - PMID:19805544
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:11161816
      supporting_text: SH3GLB, a new endophilin-related protein family featuring an SH3 domain
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Homodimer, and heterodimer with SH3GLB2
    - reference_id: PMID:19805544
      supporting_text: Endo B1 oligomerization is induced by Bax
- term:
    id: GO:0042802
    label: identical protein binding
  evidence_type: IPI
  original_reference_id: PMID:25416956
  qualifier: enables
  review:
    summary: Identical protein binding is too generic for SH3GLB1 oligomerization biology.
    action: MODIFY
    reason: SH3GLB1 forms homodimers/oligomers as part of endophilin membrane-curvature function; the 
      homodimerization term is more specific.
    proposed_replacement_terms:
    - id: GO:0042803
      label: protein homodimerization activity
    additional_reference_ids:
    - PMID:11161816
    - PMID:19805544
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:11161816
      supporting_text: SH3GLB, a new endophilin-related protein family featuring an SH3 domain
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Homodimer, and heterodimer with SH3GLB2
    - reference_id: PMID:19805544
      supporting_text: Endo B1 oligomerization is induced by Bax
- term:
    id: GO:0042802
    label: identical protein binding
  evidence_type: IPI
  original_reference_id: PMID:32296183
  qualifier: enables
  review:
    summary: Identical protein binding is too generic for SH3GLB1 oligomerization biology.
    action: MODIFY
    reason: SH3GLB1 forms homodimers/oligomers as part of endophilin membrane-curvature function; the 
      homodimerization term is more specific.
    proposed_replacement_terms:
    - id: GO:0042803
      label: protein homodimerization activity
    additional_reference_ids:
    - PMID:11161816
    - PMID:19805544
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:11161816
      supporting_text: SH3GLB, a new endophilin-related protein family featuring an SH3 domain
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Homodimer, and heterodimer with SH3GLB2
    - reference_id: PMID:19805544
      supporting_text: Endo B1 oligomerization is induced by Bax
- term:
    id: GO:0005504
    label: fatty acid binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: enables
  review:
    summary: Fatty acid binding is supported as older endophilin B isoform biochemistry but is non-core.
    action: KEEP_AS_NON_CORE
    reason: The evidence is tied to endophilin B1 lipid binding/acyltransferase properties and brain-specific 
      splice variants, not the central PN autophagy role.
    additional_reference_ids:
    - PMID:12456676
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:12456676
      supporting_text: binds to palmitoyl-CoA
    - reference_id: PMID:12456676
      supporting_text: exhibits lysophosphatidic acid acyl transferase activity
    - reference_id: PMID:12456676
      supporting_text: brain-specific
- term:
    id: GO:0010508
    label: positive regulation of autophagy
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: involved_in
  review:
    summary: Positive regulation of autophagy is a core SH3GLB1/Bif-1 annotation.
    action: ACCEPT
    reason: Bif-1 activates PI3KC3 through UVRAG/BECN1 context, localizes to autophagosomes, and loss of Bif-1
      suppresses autophagosome formation.
    additional_reference_ids:
    - PMID:17891140
    - PMID:21068542
    - PMID:21499257
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:17891140
      supporting_text: functions as a positive mediator of the class III PI(3) kinase (PI(3)KC3)
    - reference_id: PMID:17891140
      supporting_text: loss of Bif-1 suppresses autophagosome formation
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Activates lipid kinase activity of PIK3C3
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: probably by associating with the PI3K complex II
    - reference_id: PMID:21068542
      supporting_text: tubulation and fragmentation of Atg9-positive Golgi membranes
    - reference_id: PMID:21068542
      supporting_text: Bif-1/Endophilin B1 and the PI3KC3 complex II
- term:
    id: GO:0016020
    label: membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: located_in
  review:
    summary: Membrane is true but too broad for a peripheral N-BAR autophagy/mitochondrial membrane factor.
    action: MODIFY
    reason: 'Use the supported specific membrane locations: Golgi membrane, autophagosome membrane, and mitochondrial
      outer membrane, with the mitochondrial branch retained as non-core.'
    proposed_replacement_terms:
    - id: GO:0000139
      label: Golgi membrane
    - id: GO:0000421
      label: autophagosome membrane
    - id: GO:0005741
      label: mitochondrial outer membrane
    additional_reference_ids:
    - PMID:17891140
    - PMID:21068542
    - PMID:15452144
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Golgi apparatus membrane
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Mitochondrion outer membrane
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Cytoplasmic vesicle, autophagosome
- term:
    id: GO:0032991
    label: protein-containing complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: part_of
  review:
    summary: Protein-containing complex is too generic for SH3GLB1 in the PI3KC3-C2 branch.
    action: MODIFY
    reason: Bif-1 associates with UVRAG/BECN1/PI3KC3-C2; the type II class III PI3K complex term is the 
      appropriate PN-specific replacement.
    proposed_replacement_terms:
    - id: GO:0034272
      label: phosphatidylinositol 3-kinase complex, class III, type II
    additional_reference_ids:
    - PMID:17891140
    - PMID:20643123
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:17891140
      supporting_text: interacts with Beclin 1 through ultraviolet irradiation resistance-associated gene 
        (UVRAG)
    - reference_id: PMID:20643123
      supporting_text: specific sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and BIF-1
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG; UVRAG bridges the interaction to BECN1
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: complex II (PI3KC3-C2)
- term:
    id: GO:0042171
    label: lysophosphatidic acid acyltransferase activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: enables
  review:
    summary: Lysophosphatidic acid acyltransferase activity is supported for endophilin B1 isoform/family 
      biochemistry but is non-core.
    action: KEEP_AS_NON_CORE
    reason: This activity is not the central proteostasis/autophagy function and should not override the 
      stronger Bif-1/PI3KC3-C2 membrane-fission evidence.
    additional_reference_ids:
    - PMID:12456676
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:12456676
      supporting_text: binds to palmitoyl-CoA
    - reference_id: PMID:12456676
      supporting_text: exhibits lysophosphatidic acid acyl transferase activity
    - reference_id: PMID:12456676
      supporting_text: brain-specific
- term:
    id: GO:0048102
    label: autophagic cell death
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: involved_in
  review:
    summary: Autophagic cell death overstates the supported Bif-1 autophagy/apoptosis evidence.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports positive regulation of autophagy/autophagosome assembly and a distinct 
      Bax/Bak mitochondrial apoptosis branch; direct autophagic cell death is a less precise and potentially 
      misleading term.
    proposed_replacement_terms:
    - id: GO:0010508
      label: positive regulation of autophagy
    - id: GO:2000786
      label: positive regulation of autophagosome assembly
    additional_reference_ids:
    - PMID:17891140
    - PMID:16227588
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:17891140
      supporting_text: functions as a positive mediator of the class III PI(3) kinase (PI(3)KC3)
    - reference_id: PMID:17891140
      supporting_text: loss of Bif-1 suppresses autophagosome formation
    - reference_id: PMID:16227588
      supporting_text: interacts with Bax
    - reference_id: PMID:16227588
      supporting_text: important component of the mitochondrial pathway for apoptosis
- term:
    id: GO:0141038
    label: phosphatidylinositol 3-kinase activator activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: enables
  review:
    summary: PI3K activator activity is core for SH3GLB1/Bif-1.
    action: ACCEPT
    reason: Bif-1 activates PI3KC3 via the UVRAG/Beclin1 PI3KC3-C2 context during autophagy.
    additional_reference_ids:
    - PMID:17891140
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:17891140
      supporting_text: functions as a positive mediator of the class III PI(3) kinase (PI(3)KC3)
    - reference_id: PMID:17891140
      supporting_text: loss of Bif-1 suppresses autophagosome formation
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Activates lipid kinase activity of PIK3C3
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: probably by associating with the PI3K complex II
    - reference_id: PMID:17891140
      supporting_text: interacts with Beclin 1 through ultraviolet irradiation resistance-associated gene 
        (UVRAG)
    - reference_id: PMID:20643123
      supporting_text: specific sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and BIF-1
- term:
    id: GO:1903778
    label: protein localization to vacuolar membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: involved_in
  review:
    summary: Protein localization to vacuolar membrane should be humanized/narrowed to autophagosome membrane 
      and autophagosome assembly.
    action: MODIFY
    reason: The human evidence is Atg9 trafficking from Golgi membranes toward autophagosome biogenesis, not a
      generic vacuolar membrane localization process.
    proposed_replacement_terms:
    - id: GO:2000786
      label: positive regulation of autophagosome assembly
    - id: GO:0000421
      label: autophagosome membrane
    additional_reference_ids:
    - PMID:21068542
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:21068542
      supporting_text: tubulation and fragmentation of Atg9-positive Golgi membranes
    - reference_id: PMID:21068542
      supporting_text: Bif-1/Endophilin B1 and the PI3KC3 complex II
    - reference_id: PMID:21068542
      supporting_text: the fission of Golgi membranes and the trafficking of Atg9 from the Golgi complex to 
        autophagosomes
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: regulate the trafficking of ATG9A from the Golgi complex
- term:
    id: GO:2000786
    label: positive regulation of autophagosome assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  qualifier: involved_in
  review:
    summary: Positive regulation of autophagosome assembly is a core SH3GLB1 process.
    action: ACCEPT
    reason: Bif-1/Endophilin B1 promotes Atg9-positive Golgi membrane fission and PI3KC3-C2-dependent 
      autophagosome biogenesis.
    additional_reference_ids:
    - PMID:17891140
    - PMID:21068542
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:17891140
      supporting_text: functions as a positive mediator of the class III PI(3) kinase (PI(3)KC3)
    - reference_id: PMID:17891140
      supporting_text: loss of Bif-1 suppresses autophagosome formation
    - reference_id: PMID:21068542
      supporting_text: tubulation and fragmentation of Atg9-positive Golgi membranes
    - reference_id: PMID:21068542
      supporting_text: Bif-1/Endophilin B1 and the PI3KC3 complex II
    - reference_id: PMID:21068542
      supporting_text: the fission of Golgi membranes and the trafficking of Atg9 from the Golgi complex to 
        autophagosomes
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: regulate the trafficking of ATG9A from the Golgi complex
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: IDA
  original_reference_id: GO_REF:0000052
  qualifier: located_in
  review:
    summary: Cytoplasmic/cytosolic localization is broad but compatible with SH3GLB1.
    action: KEEP_AS_NON_CORE
    reason: More specific membrane locations drive the functional review; cytoplasm/cytosol is retained as 
      broad context only.
    proposed_replacement_terms:
    - id: GO:0000139
      label: Golgi membrane
    - id: GO:0000421
      label: autophagosome membrane
    - id: GO:0005741
      label: mitochondrial outer membrane
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Cytoplasm {ECO:0000269|PubMed:15452144}
- term:
    id: GO:0000139
    label: Golgi membrane
  evidence_type: EXP
  original_reference_id: PMID:21068542
  qualifier: located_in
  review:
    summary: Golgi membrane localization is core for Bif-1-mediated ATG9 trafficking.
    action: ACCEPT
    reason: SH3GLB1 localizes to Golgi membranes and drives starvation-induced Atg9-positive Golgi membrane 
      fission for autophagosome biogenesis.
    additional_reference_ids:
    - PMID:21068542
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:21068542
      supporting_text: tubulation and fragmentation of Atg9-positive Golgi membranes
    - reference_id: PMID:21068542
      supporting_text: Bif-1/Endophilin B1 and the PI3KC3 complex II
    - reference_id: PMID:21068542
      supporting_text: the fission of Golgi membranes and the trafficking of Atg9 from the Golgi complex to 
        autophagosomes
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: regulate the trafficking of ATG9A from the Golgi complex
- term:
    id: GO:0031334
    label: positive regulation of protein-containing complex assembly
  evidence_type: IDA
  original_reference_id: PMID:19074440
  qualifier: involved_in
  review:
    summary: Positive regulation of protein-containing complex assembly is supported in Bax activation context
      but non-core.
    action: KEEP_AS_NON_CORE
    reason: This row reflects Bif-1/Bax apoptotic membrane-permeabilization biology rather than the PN 
      autophagy core.
    additional_reference_ids:
    - PMID:19074440
    - PMID:16227588
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:19074440
      supporting_text: stimulatory effect of Bif-1 on BAX-driven MOMP
    - reference_id: PMID:19074440
      supporting_text: BAX conformational activation
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
- term:
    id: GO:0032991
    label: protein-containing complex
  evidence_type: IDA
  original_reference_id: PMID:19805544
  qualifier: part_of
  review:
    summary: Protein-containing complex is too generic for Bax-induced Endo B1 oligomerization.
    action: MODIFY
    reason: The cited evidence supports Endo B1 oligomerization/lipid vesiculation in apoptosis, not a stable 
      named complex term.
    proposed_replacement_terms:
    - id: GO:0042803
      label: protein homodimerization activity
    additional_reference_ids:
    - PMID:19805544
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:19805544
      supporting_text: Endo B1 oligomerization is induced by Bax
    - reference_id: PMID:19805544
      supporting_text: massive vesiculation of liposomes
- term:
    id: GO:0045296
    label: cadherin binding
  evidence_type: HDA
  original_reference_id: PMID:25468996
  qualifier: enables
  review:
    summary: Cadherin binding is a high-throughput interaction over-annotation for SH3GLB1.
    action: MARK_AS_OVER_ANNOTATED
    reason: The HDA interactome row does not define SH3GLB1 core function; no supporting SH3GLB1 cadherin 
      mechanism is evident from the curated functional record.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Endophilin-B1
- term:
    id: GO:0031647
    label: regulation of protein stability
  evidence_type: HMP
  original_reference_id: PMID:24270810
  qualifier: involved_in
  review:
    summary: Regulation of protein stability from a high-throughput mitophagy screen is not a core SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The main text describes a genome-wide resource and mechanistic follow-up of other genes; 
      SH3GLB1-specific functional support is better captured by mitochondrial membrane dynamics and autophagy 
      membrane fission.
    additional_reference_ids:
    - PMID:24270810
    - PMID:15452144
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:24270810
      supporting_text: genome-wide small interfering RNA (siRNA) screens
    - reference_id: PMID:15452144
      supporting_text: required for maintenance of mitochondrial morphology
- term:
    id: GO:1903749
    label: positive regulation of protein localization to mitochondrion
  evidence_type: HMP
  original_reference_id: PMID:24270810
  qualifier: involved_in
  review:
    summary: Positive regulation of protein localization to mitochondrion is a high-throughput 
      mitophagy-screen overextension.
    action: MARK_AS_OVER_ANNOTATED
    reason: SH3GLB1 has supported mitochondrial outer membrane dynamics, but this HMP process term is not the 
      best representation of its function.
    proposed_replacement_terms:
    - id: GO:0005741
      label: mitochondrial outer membrane
    additional_reference_ids:
    - PMID:24270810
    - PMID:15452144
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:24270810
      supporting_text: parkin translocation to damaged mitochondria
    - reference_id: PMID:15452144
      supporting_text: required for the regulation of the outer mitochondrial membrane dynamics
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IDA
  original_reference_id: PMID:25468996
  qualifier: located_in
  review:
    summary: Cytoplasmic/cytosolic localization is broad but compatible with SH3GLB1.
    action: KEEP_AS_NON_CORE
    reason: More specific membrane locations drive the functional review; cytoplasm/cytosol is retained as 
      broad context only.
    proposed_replacement_terms:
    - id: GO:0000139
      label: Golgi membrane
    - id: GO:0000421
      label: autophagosome membrane
    - id: GO:0005741
      label: mitochondrial outer membrane
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Cytoplasm {ECO:0000269|PubMed:15452144}
- term:
    id: GO:0016241
    label: regulation of macroautophagy
  evidence_type: TAS
  original_reference_id: PMID:21499257
  qualifier: involved_in
  review:
    summary: Regulation of macroautophagy is supported and core but less specific than the PI3KC3-C2/ATG9 
      mechanism.
    action: ACCEPT
    reason: Cdk5 phosphorylation and Bif-1/PI3KC3-C2 studies support induced autophagy; the detailed mechanism
      is positive regulation of autophagosome assembly.
    additional_reference_ids:
    - PMID:21499257
    - PMID:17891140
    - PMID:21068542
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:21499257
      supporting_text: required for induced autophagy
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: required for autophagy induction in starved neurons
    - reference_id: PMID:17891140
      supporting_text: functions as a positive mediator of the class III PI(3) kinase (PI(3)KC3)
    - reference_id: PMID:17891140
      supporting_text: loss of Bif-1 suppresses autophagosome formation
- term:
    id: GO:0034198
    label: cellular response to amino acid starvation
  evidence_type: IMP
  original_reference_id: PMID:21068542
  qualifier: involved_in
  review:
    summary: Amino-acid starvation response is the experimental context for Bif-1 autophagy induction and is 
      non-core.
    action: KEEP_AS_NON_CORE
    reason: The functional annotation should emphasize ATG9 trafficking/autophagosome assembly rather than the
      starvation stimulus itself.
    additional_reference_ids:
    - PMID:21068542
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:21068542
      supporting_text: tubulation and fragmentation of Atg9-positive Golgi membranes
    - reference_id: PMID:21068542
      supporting_text: Bif-1/Endophilin B1 and the PI3KC3 complex II
    - reference_id: PMID:21068542
      supporting_text: the fission of Golgi membranes and the trafficking of Atg9 from the Golgi complex to 
        autophagosomes
- term:
    id: GO:0042149
    label: cellular response to glucose starvation
  evidence_type: IMP
  original_reference_id: PMID:21068542
  qualifier: involved_in
  review:
    summary: Glucose starvation response is an experimental context for Bif-1 autophagy induction and is 
      non-core.
    action: KEEP_AS_NON_CORE
    reason: The core process is starvation-induced ATG9/Golgi membrane fission and autophagosome assembly, not
      glucose starvation response per se.
    additional_reference_ids:
    - PMID:21068542
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:21068542
      supporting_text: tubulation and fragmentation of Atg9-positive Golgi membranes
    - reference_id: PMID:21068542
      supporting_text: Bif-1/Endophilin B1 and the PI3KC3 complex II
    - reference_id: PMID:21068542
      supporting_text: the fission of Golgi membranes and the trafficking of Atg9 from the Golgi complex to 
        autophagosomes
- term:
    id: GO:0090148
    label: membrane fission
  evidence_type: IMP
  original_reference_id: PMID:21068542
  qualifier: involved_in
  review:
    summary: Membrane fission is a core Bif-1/endophilin B1 process in ATG9 trafficking.
    action: ACCEPT
    reason: Bif-1 N-BAR-dependent Golgi membrane fission supplies Atg9-positive membranes for autophagosome 
      formation.
    additional_reference_ids:
    - PMID:11604418
    - PMID:21068542
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:11604418
      supporting_text: directly bind and evaginate lipid bilayers into narrow tubules
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: induce membrane curvature and bind curved membranes
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: N-BAR domain
    - reference_id: PMID:21068542
      supporting_text: tubulation and fragmentation of Atg9-positive Golgi membranes
    - reference_id: PMID:21068542
      supporting_text: Bif-1/Endophilin B1 and the PI3KC3 complex II
    - reference_id: PMID:21068542
      supporting_text: the fission of Golgi membranes and the trafficking of Atg9 from the Golgi complex to 
        autophagosomes
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: regulate the trafficking of ATG9A from the Golgi complex
- term:
    id: GO:1903527
    label: positive regulation of membrane tubulation
  evidence_type: IMP
  original_reference_id: PMID:21068542
  qualifier: involved_in
  review:
    summary: Positive regulation of membrane tubulation is core for the N-BAR domain-mediated autophagy 
      membrane source role.
    action: ACCEPT
    reason: The N-BAR domain drives membrane binding/tubulation, and Bif-1-dependent Golgi 
      tubulation/fragmentation supports ATG9 trafficking during autophagy.
    additional_reference_ids:
    - PMID:11604418
    - PMID:21068542
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:11604418
      supporting_text: directly bind and evaginate lipid bilayers into narrow tubules
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: induce membrane curvature and bind curved membranes
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: N-BAR domain
    - reference_id: PMID:21068542
      supporting_text: tubulation and fragmentation of Atg9-positive Golgi membranes
    - reference_id: PMID:21068542
      supporting_text: Bif-1/Endophilin B1 and the PI3KC3 complex II
- term:
    id: GO:1903778
    label: protein localization to vacuolar membrane
  evidence_type: IMP
  original_reference_id: PMID:21068542
  qualifier: involved_in
  review:
    summary: Protein localization to vacuolar membrane should be humanized/narrowed to autophagosome membrane 
      and autophagosome assembly.
    action: MODIFY
    reason: The human evidence is Atg9 trafficking from Golgi membranes toward autophagosome biogenesis, not a
      generic vacuolar membrane localization process.
    proposed_replacement_terms:
    - id: GO:2000786
      label: positive regulation of autophagosome assembly
    - id: GO:0000421
      label: autophagosome membrane
    additional_reference_ids:
    - PMID:21068542
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:21068542
      supporting_text: tubulation and fragmentation of Atg9-positive Golgi membranes
    - reference_id: PMID:21068542
      supporting_text: Bif-1/Endophilin B1 and the PI3KC3 complex II
    - reference_id: PMID:21068542
      supporting_text: the fission of Golgi membranes and the trafficking of Atg9 from the Golgi complex to 
        autophagosomes
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: regulate the trafficking of ATG9A from the Golgi complex
- term:
    id: GO:2000786
    label: positive regulation of autophagosome assembly
  evidence_type: IMP
  original_reference_id: PMID:21068542
  qualifier: involved_in
  review:
    summary: Positive regulation of autophagosome assembly is a core SH3GLB1 process.
    action: ACCEPT
    reason: Bif-1/Endophilin B1 promotes Atg9-positive Golgi membrane fission and PI3KC3-C2-dependent 
      autophagosome biogenesis.
    additional_reference_ids:
    - PMID:17891140
    - PMID:21068542
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:17891140
      supporting_text: functions as a positive mediator of the class III PI(3) kinase (PI(3)KC3)
    - reference_id: PMID:17891140
      supporting_text: loss of Bif-1 suppresses autophagosome formation
    - reference_id: PMID:21068542
      supporting_text: tubulation and fragmentation of Atg9-positive Golgi membranes
    - reference_id: PMID:21068542
      supporting_text: Bif-1/Endophilin B1 and the PI3KC3 complex II
    - reference_id: PMID:21068542
      supporting_text: the fission of Golgi membranes and the trafficking of Atg9 from the Golgi complex to 
        autophagosomes
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: regulate the trafficking of ATG9A from the Golgi complex
- term:
    id: GO:0000421
    label: autophagosome membrane
  evidence_type: IDA
  original_reference_id: PMID:17891140
  qualifier: located_in
  review:
    summary: Autophagosome membrane localization is core for SH3GLB1/Bif-1 autophagy function.
    action: ACCEPT
    reason: Bif-1 localizes to autophagosomes after nutrient deprivation and supports autophagosome formation 
      through UVRAG/PI3KC3-C2.
    additional_reference_ids:
    - PMID:17891140
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:17891140
      supporting_text: localizes to autophagosomes
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Cytoplasmic vesicle, autophagosome
    - reference_id: PMID:17891140
      supporting_text: functions as a positive mediator of the class III PI(3) kinase (PI(3)KC3)
    - reference_id: PMID:17891140
      supporting_text: loss of Bif-1 suppresses autophagosome formation
- term:
    id: GO:0010508
    label: positive regulation of autophagy
  evidence_type: IMP
  original_reference_id: PMID:17891140
  qualifier: involved_in
  review:
    summary: Positive regulation of autophagy is a core SH3GLB1/Bif-1 annotation.
    action: ACCEPT
    reason: Bif-1 activates PI3KC3 through UVRAG/BECN1 context, localizes to autophagosomes, and loss of Bif-1
      suppresses autophagosome formation.
    additional_reference_ids:
    - PMID:17891140
    - PMID:21068542
    - PMID:21499257
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:17891140
      supporting_text: functions as a positive mediator of the class III PI(3) kinase (PI(3)KC3)
    - reference_id: PMID:17891140
      supporting_text: loss of Bif-1 suppresses autophagosome formation
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Activates lipid kinase activity of PIK3C3
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: probably by associating with the PI3K complex II
    - reference_id: PMID:21068542
      supporting_text: tubulation and fragmentation of Atg9-positive Golgi membranes
    - reference_id: PMID:21068542
      supporting_text: Bif-1/Endophilin B1 and the PI3KC3 complex II
- term:
    id: GO:0030496
    label: midbody
  evidence_type: IDA
  original_reference_id: PMID:20643123
  qualifier: located_in
  review:
    summary: Midbody localization is supported for the PI3KC3-C2/BIF-1 cytokinesis branch but non-core.
    action: KEEP_AS_NON_CORE
    reason: The cytokinesis/midbody role is real but separate from the autophagy/ATG9 membrane fission core.
    additional_reference_ids:
    - PMID:20643123
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:20643123
      supporting_text: regulates both receptor degradation and cytokinesis
    - reference_id: PMID:20643123
      supporting_text: strong localisation of these proteins to the midbody
- term:
    id: GO:0032465
    label: regulation of cytokinesis
  evidence_type: IMP
  original_reference_id: PMID:20643123
  qualifier: involved_in
  review:
    summary: Regulation of cytokinesis is supported but non-core.
    action: KEEP_AS_NON_CORE
    reason: A PI3KC3-C2/BIF-1-containing subcomplex regulates cytokinesis and receptor degradation, but this 
      is outside the main autophagy membrane fission role.
    additional_reference_ids:
    - PMID:20643123
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:20643123
      supporting_text: regulates both receptor degradation and cytokinesis
    - reference_id: PMID:20643123
      supporting_text: strong localisation of these proteins to the midbody
- term:
    id: GO:0032801
    label: receptor catabolic process
  evidence_type: IMP
  original_reference_id: PMID:20643123
  qualifier: involved_in
  review:
    summary: Receptor catabolic process is supported but non-core.
    action: KEEP_AS_NON_CORE
    reason: BIF-1-containing PI3KC3-C2 contributes to degradative endocytic traffic, but the PN core is 
      autophagy/ATG9 membrane fission.
    additional_reference_ids:
    - PMID:20643123
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:20643123
      supporting_text: regulates both receptor degradation and cytokinesis
    - reference_id: PMID:20643123
      supporting_text: growth factor receptor degradation
- term:
    id: GO:0048102
    label: autophagic cell death
  evidence_type: IMP
  original_reference_id: PMID:17891140
  qualifier: involved_in
  review:
    summary: Autophagic cell death overstates the supported Bif-1 autophagy/apoptosis evidence.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports positive regulation of autophagy/autophagosome assembly and a distinct 
      Bax/Bak mitochondrial apoptosis branch; direct autophagic cell death is a less precise and potentially 
      misleading term.
    proposed_replacement_terms:
    - id: GO:0010508
      label: positive regulation of autophagy
    - id: GO:2000786
      label: positive regulation of autophagosome assembly
    additional_reference_ids:
    - PMID:17891140
    - PMID:16227588
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:17891140
      supporting_text: functions as a positive mediator of the class III PI(3) kinase (PI(3)KC3)
    - reference_id: PMID:17891140
      supporting_text: loss of Bif-1 suppresses autophagosome formation
    - reference_id: PMID:16227588
      supporting_text: interacts with Bax
    - reference_id: PMID:16227588
      supporting_text: important component of the mitochondrial pathway for apoptosis
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:11161816
  qualifier: enables
  review:
    summary: The interaction evidence may be real, but generic protein binding is not an informative SH3GLB1 
      function.
    action: MARK_AS_OVER_ANNOTATED
    reason: Replace generic protein binding with specific adaptor activity, PI3KC3-C2 association, 
      homodimerization, membrane curvature/fission, or Bax/mitochondrial apoptosis context as appropriate.
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds BAX
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Interacts with UVRAG
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IDA
  original_reference_id: PMID:11161816
  qualifier: located_in
  review:
    summary: Cytoplasmic/cytosolic localization is broad but compatible with SH3GLB1.
    action: KEEP_AS_NON_CORE
    reason: More specific membrane locations drive the functional review; cytoplasm/cytosol is retained as 
      broad context only.
    proposed_replacement_terms:
    - id: GO:0000139
      label: Golgi membrane
    - id: GO:0000421
      label: autophagosome membrane
    - id: GO:0005741
      label: mitochondrial outer membrane
    additional_reference_ids:
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Cytoplasm {ECO:0000269|PubMed:15452144}
- term:
    id: GO:0042803
    label: protein homodimerization activity
  evidence_type: IPI
  original_reference_id: PMID:11161816
  qualifier: enables
  review:
    summary: Protein homodimerization is supported and relevant to endophilin BAR-domain membrane remodeling.
    action: ACCEPT
    reason: SH3GLB1 homodimer/oligomer formation is part of the endophilin membrane-curvature mechanism and is
      more specific than identical protein binding.
    additional_reference_ids:
    - PMID:11161816
    - PMID:19805544
    - file:human/SH3GLB1/SH3GLB1-uniprot.txt
    - file:human/SH3GLB1/SH3GLB1-notes.md
    supported_by:
    - reference_id: PMID:11161816
      supporting_text: SH3GLB, a new endophilin-related protein family featuring an SH3 domain
    - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
      supporting_text: Homodimer, and heterodimer with SH3GLB2
    - reference_id: PMID:19805544
      supporting_text: Endo B1 oligomerization is induced by Bax
references:
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
- id: GO_REF:0000044
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, 
    accompanied by conservative changes to GO terms applied by UniProt
  findings: []
- id: GO_REF:0000052
  title: Gene Ontology annotation based on curation of immunofluorescence data
  findings: []
- id: GO_REF:0000107
  title: Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl 
    Compara
  findings: []
- id: GO_REF:0000120
  title: Combined Automated Annotation using Multiple IEA Methods
  findings: []
- id: PMID:11161816
  title: SH3GLB, a new endophilin-related protein family featuring an SH3 domain.
  findings: []
- id: PMID:17891140
  title: Bif-1 interacts with Beclin 1 through UVRAG and regulates autophagy and tumorigenesis.
  findings: []
- id: PMID:19074440
  title: Endophilin B1/Bif-1 stimulates BAX activation independently from its capacity to produce large scale 
    membrane morphological rearrangements.
  findings: []
- id: PMID:19805544
  title: Bax activates endophilin B1 oligomerization and lipid membrane vesiculation.
  findings: []
- id: PMID:20562859
  title: Network organization of the human autophagy system.
  findings: []
- id: PMID:20643123
  title: A phosphatidylinositol 3-kinase class III sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and 
    BIF-1 regulates cytokinesis and degradative endocytic traffic.
  findings: []
- id: PMID:21068542
  title: Bif-1 regulates Atg9 trafficking by mediating the fission of Golgi membranes during autophagy.
  findings: []
- id: PMID:21499257
  title: Cdk5-mediated phosphorylation of endophilin B1 is required for induced autophagy in models of 
    Parkinson's disease.
  findings: []
- id: PMID:21988832
  title: Toward an understanding of the protein interaction network of the human liver.
  findings: []
- id: PMID:23414517
  title: 'A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome.'
  findings: []
- id: PMID:24056303
  title: PtdIns(3)P-bound UVRAG coordinates Golgi-ER retrograde and Atg9 transport by differential 
    interactions with the ER tether and the beclin 1 complex.
  findings: []
- id: PMID:24270810
  title: High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
  findings: []
- id: PMID:25416956
  title: A proteome-scale map of the human interactome network.
  findings: []
- id: PMID:25468996
  title: E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
  findings: []
- id: PMID:25910212
  title: Widespread macromolecular interaction perturbations in human genetic disorders.
  findings: []
- id: PMID:27107012
  title: Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
  findings: []
- id: PMID:31515488
  title: Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum
    in human populations.
  findings: []
- id: PMID:32296183
  title: A reference map of the human binary protein interactome.
  findings: []
- id: PMID:32814053
  title: Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread 
    Protein Aggregation in Affected Brains.
  findings: []
- id: PMID:33961781
  title: Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
  findings: []
- id: PMID:35271311
  title: 'OpenCell: Endogenous tagging for the cartography of human cellular organization.'
  findings: []
- id: PMID:39526800
  title: A comprehensive two-hybrid analysis to explore the Legionella pneumophila effector-effector 
    interactome.
  findings: []
- id: PMID:40205054
  title: Multimodal cell maps as a foundation for structural and functional genomics.
  findings: []
- id: PMID:11259440
  title: Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein 
    that associates with Bax.
  findings: []
- id: PMID:11604418
  title: Generation of high curvature membranes mediated by direct endophilin bilayer interactions.
  findings: []
- id: PMID:12456676
  title: Characterization of Endophilin B1b, a brain-specific membrane-associated lysophosphatidic acid acyl 
    transferase with properties distinct from endophilin A1.
  findings: []
- id: PMID:15452144
  title: Endophilin B1 is required for the maintenance of mitochondrial morphology.
  findings: []
- id: PMID:16227588
  title: Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis.
  findings: []
- id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
  title: UniProtKB record for human SH3GLB1
  findings: []
- id: file:human/SH3GLB1/SH3GLB1-notes.md
  title: SH3GLB1 review notes
  findings: []
core_functions:
- molecular_function:
    id: GO:0030674
    label: protein-macromolecule adaptor activity
  description: SH3GLB1/Bif-1 acts as a BAR/SH3 adaptor that connects UVRAG/BECN1/PI3KC3-C2 to autophagy 
    membrane remodeling and ATG9-positive Golgi membrane trafficking.
  directly_involved_in:
  - id: GO:0010508
    label: positive regulation of autophagy
  - id: GO:2000786
    label: positive regulation of autophagosome assembly
  - id: GO:0090148
    label: membrane fission
  - id: GO:1903527
    label: positive regulation of membrane tubulation
  locations:
  - id: GO:0000139
    label: Golgi membrane
  - id: GO:0000421
    label: autophagosome membrane
  in_complex:
    id: GO:0034272
    label: phosphatidylinositol 3-kinase complex, class III, type II
  supported_by:
  - &id005
    reference_id: PMID:17891140
    supporting_text: interacts with Beclin 1 through ultraviolet irradiation resistance-associated gene 
      (UVRAG)
  - &id006
    reference_id: PMID:20643123
    supporting_text: specific sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and BIF-1
  - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
    supporting_text: Interacts with UVRAG; UVRAG bridges the interaction to BECN1
  - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
    supporting_text: complex II (PI3KC3-C2)
  - &id001
    reference_id: PMID:17891140
    supporting_text: functions as a positive mediator of the class III PI(3) kinase (PI(3)KC3)
  - &id002
    reference_id: PMID:17891140
    supporting_text: loss of Bif-1 suppresses autophagosome formation
  - &id003
    reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
    supporting_text: Activates lipid kinase activity of PIK3C3
  - &id004
    reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
    supporting_text: probably by associating with the PI3K complex II
  - reference_id: PMID:21068542
    supporting_text: tubulation and fragmentation of Atg9-positive Golgi membranes
  - reference_id: PMID:21068542
    supporting_text: Bif-1/Endophilin B1 and the PI3KC3 complex II
  - reference_id: PMID:21068542
    supporting_text: the fission of Golgi membranes and the trafficking of Atg9 from the Golgi complex to 
      autophagosomes
  - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
    supporting_text: regulate the trafficking of ATG9A from the Golgi complex
- molecular_function:
    id: GO:0141038
    label: phosphatidylinositol 3-kinase activator activity
  description: Bif-1 positively regulates PIK3C3/VPS34 lipid kinase activity through the UVRAG-containing 
    PI3KC3-C2 autophagy complex.
  directly_involved_in:
  - id: GO:0010508
    label: positive regulation of autophagy
  - id: GO:2000786
    label: positive regulation of autophagosome assembly
  locations:
  - id: GO:0000421
    label: autophagosome membrane
  in_complex:
    id: GO:0034272
    label: phosphatidylinositol 3-kinase complex, class III, type II
  supported_by:
  - *id001
  - *id002
  - *id003
  - *id004
  - *id005
  - *id006
- molecular_function:
    id: GO:0042803
    label: protein homodimerization activity
  description: Endophilin B1 dimerization/oligomerization supports N-BAR membrane curvature, tubulation, and 
    fission functions relevant to autophagy membrane supply and mitochondrial membrane remodeling.
  directly_involved_in:
  - id: GO:1903527
    label: positive regulation of membrane tubulation
  - id: GO:0090148
    label: membrane fission
  locations:
  - id: GO:0000139
    label: Golgi membrane
  - id: GO:0005741
    label: mitochondrial outer membrane
  supported_by:
  - reference_id: PMID:11161816
    supporting_text: SH3GLB, a new endophilin-related protein family featuring an SH3 domain
  - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
    supporting_text: Homodimer, and heterodimer with SH3GLB2
  - reference_id: PMID:19805544
    supporting_text: Endo B1 oligomerization is induced by Bax
  - reference_id: PMID:11604418
    supporting_text: directly bind and evaginate lipid bilayers into narrow tubules
  - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
    supporting_text: induce membrane curvature and bind curved membranes
  - reference_id: file:human/SH3GLB1/SH3GLB1-uniprot.txt
    supporting_text: N-BAR domain
proposed_new_terms: []
suggested_questions:
- question: Should SH3GLB1 be directly curated to GO:0034272 class III PI3K complex type II, or only to PI3KC3
    activator/adaptor activity with PI3KC3-C2 context?
  experts:
  - GO autophagy editors
  - ComplexPortal curators
  - Reactome autophagy curators
- question: Is membrane fission or positive regulation of membrane tubulation the best GO process for 
    Bif-1-mediated Atg9-positive Golgi membrane remodeling during autophagosome assembly?
  experts:
  - GO autophagy editors
  - membrane trafficking experts
- question: Should autophagic cell death annotations for Bif-1 be replaced by positive regulation of autophagy
    plus separate Bax/Bak mitochondrial apoptosis annotations?
  experts:
  - GO apoptosis editors
  - GO autophagy editors
suggested_experiments:
- description: Rescue SH3GLB1 knockout cells with N-BAR, SH3, and dimerization mutants and measure PI3KC3 
    activity, Atg9 redistribution, Golgi membrane fission, and LC3/WIPI autophagosome assembly.
  experiment_type: Bif-1 autophagy membrane-fission rescue assay
  hypothesis: Bif-1 requires both N-BAR membrane remodeling and SH3-dependent UVRAG/PI3KC3-C2 engagement to 
    support Atg9 trafficking and autophagosome assembly.
- description: Compare wild type and Bax-binding/mitochondrial-targeting SH3GLB1 mutants for mitochondrial 
    morphology, Bax/Bak activation, and autophagic flux in the same background.
  experiment_type: separation-of-function apoptosis versus autophagy assay
  hypothesis: SH3GLB1 mitochondrial apoptosis and PI3KC3-C2 autophagy roles are genetically separable.