SLC10A1 (NTCP, Na+/taurocholate cotransporting polypeptide) is the principal sodium-dependent bile salt uptake carrier of the mammalian liver. It is a multi-pass membrane protein of the bile acid:sodium symporter (BASS) family that resides in the basolateral (sinusoidal) plasma membrane of hepatocytes, where it imports conjugated and unconjugated bile salts from portal blood into the hepatocyte in co-transport with two sodium ions, driven by the inward sodium gradient [PMID:8132774, PMID:34060352, PMID:35545671]. Cryo-EM structures show a gated-pore mechanism in which the transporter opens a wide transmembrane pathway for the bile salt [PMID:35545671]. Substrate specificity is broad for a bile salt carrier and extends to non-bile-acid organic anions such as estrone-3-sulfate and to drugs including rosuvastatin, so the protein also contributes to hepatic clearance of organic anions and xenobiotics [PMID:14660639, PMID:9458785, PMID:34060352]. NTCP forms homodimers (and heterocomplexes with other SLC10 family members) in which individual subunits are independently functional, and heteromeric assembly can limit its delivery to the plasma membrane [PMID:22029531]. By performing the hepatocyte uptake step, SLC10A1 is one of the central transporters of enterohepatic bile salt cycling [PMID:33222321]. Human loss of function causes NTCP deficiency, characterized by extreme conjugated hypercholanemia with a mild and largely non-hepatic clinical phenotype, bile salt synthesis and intestinal FGF19 signalling remaining intact [PMID:24867799]. Independently of its transport role, human NTCP is the cell-surface entry receptor for hepatitis B and hepatitis D virus, binding the preS1 domain of the large viral envelope protein, which makes it a major antiviral drug target [PMID:23150796, PMID:35545671].
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput binary interaction screen (HuRI) reports an interaction between SLC10A1 and this partner [PMID:32296183]. The annotation records only the generic term protein binding and carries no information about SLC10A1's molecular function. Reason: GO:0005515 protein binding is uninformative: it states that a binding event was observed without identifying what SLC10A1 does. The source is a systematic proteome-scale Y2H map [PMID:32296183], which assigns no function to either partner, and nothing in it supports a more specific molecular-function term for SLC10A1. Removing the annotation does not assert that the reported interaction is false; it removes a row that adds no functional knowledge beyond SLC10A1's well-established role as the sodium-dependent hepatic bile salt uptake carrier [PMID:35545671, PMID:24867799]. Supporting Evidence: PMID:32296183 Here we present a human 'all-by-all' PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0005515 protein binding | IPI PMID:40355756 The solute carrier superfamily interactome. | REMOVE | Summary: Interaction-proteomics survey of 396 solute carriers reports SLC10A1 in a complex with UniProtKB:Q8N6S5 [PMID:40355756]; the annotation uses only the generic protein binding term. Reason: As with the HuRI rows, GO:0005515 conveys no functional information. The cited study is an affinity-proteomics survey whose stated aim is a global map of SLC-protein interactions rather than functional assignment [PMID:40355756], and it provides no basis for a specific molecular-function term for SLC10A1. Removal is a statement about the uninformativeness of the term, not about the validity of the interaction. Supporting Evidence: PMID:40355756 We aimed at obtaining a global survey of the SLC-protein interaction landscape and mapped the protein-protein interactions of 396 SLCs by interaction proteomics. |
| GO:0005886 plasma membrane | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT places plasma-membrane activity for the NTCP/ASBT clade of SLC10 transporters; SLC10A1 inherits it. This matches the direct human and rodent evidence that NTCP functions in the hepatocyte basolateral plasma membrane [PMID:34060352, PMID:22029531]. Reason: The node placement is sound: sodium-dependent bile salt uptake is by definition a plasma-membrane activity, and human NTCP is directly shown to reside and act there (FRET of labelled subunits at the plasma membrane [PMID:22029531]; loss-of-function variants act by failing to reach the plasma membrane [PMID:24867799, PMID:14660639]). GO:0016323 basolateral plasma membrane is the more precise location and is separately annotated, so this parent term is appropriately retained alongside it. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000040759 SUPPORTS TRANSFER Ancestral SLC10 node for which plasma-membrane localization is experimentally grounded in both the human and rodent descendants. Supporting Evidence: PMID:22029531 FRET (fluorescence resonance energy transfer) using fluorescently labelled subunits further demonstrated that dimerization persists at the plasma membrane. PMID:24867799 Immunofluorescence studies and surface biotinylation experiments demonstrated that the mutant protein is virtually absent from the plasma membrane. PMID:34060352 The Na+/taurocholate cotransporting polypeptide (NTCP) is located in the basolateral membrane of hepatocytes, where it transports bile acids from the portal blood back into hepatocytes. |
| GO:0005886 plasma membrane | IDA PMID:22029531 Homo- and hetero-dimeric architecture of the human liver Na⁺... | ACCEPT | Summary: Direct assay: FRET between differentially tagged NTCP subunits showed that NTCP dimers are present at the plasma membrane of NTCP-expressing cells [PMID:22029531]. Reason: Localization of human NTCP to the plasma membrane is directly demonstrated in this study, and the same study shows that plasma-membrane abundance determines taurocholate uptake capacity [PMID:22029531]. This is the correct location for the gene's core transport activity. Supporting Evidence: PMID:22029531 FRET (fluorescence resonance energy transfer) using fluorescently labelled subunits further demonstrated that dimerization persists at the plasma membrane. PMID:22029531 Expression of SLC10A4 and NTCP Y307X resulted in a reduction of NTCP abundance at the plasma membrane and NTCP-mediated taurocholate uptake |
| GO:0005886 plasma membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Automatic mapping from the UniProt subcellular-location vocabulary (SL-0039, cell membrane) to GO:0005886. Reason: The underlying UniProt location assignment is correct: human NTCP is a plasma-membrane bile salt carrier, directly shown at the plasma membrane [PMID:22029531] and in the hepatocyte basolateral membrane [PMID:34060352]. The term is less precise than GO:0016323 but is not wrong, and GOA keeps both. Supporting Evidence: PMID:22029531 FRET (fluorescence resonance energy transfer) using fluorescently labelled subunits further demonstrated that dimerization persists at the plasma membrane. PMID:34060352 The Na+/taurocholate cotransporting polypeptide (NTCP) is located in the basolateral membrane of hepatocytes, where it transports bile acids from the portal blood back into hepatocytes. |
| GO:0005886 plasma membrane | TAS PMID:8132774 Molecular cloning, chromosomal localization, and functional ... | ACCEPT | Summary: Author statement from the cloning paper, which expressed human NTCP at the surface of Xenopus oocytes and measured Na+-dependent taurocholate uptake [PMID:8132774]. Reason: Uptake of taurocholate by cRNA-injected oocytes requires the transporter to be in the plasma membrane [PMID:8132774], and subsequent direct work confirms the plasma-membrane localization of human NTCP [PMID:22029531]. Supporting Evidence: PMID:8132774 Injection of in vitro transcribed cRNA into Xenopus laevis oocytes resulted in the expression of Na(+)-dependent taurocholate uptake. PMID:22029531 FRET (fluorescence resonance energy transfer) using fluorescently labelled subunits further demonstrated that dimerization persists at the plasma membrane. |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-194121 | ACCEPT | Summary: Reactome models SLC10A1-mediated co-transport of bile salt and two Na+ ions across the plasma membrane [Reactome:R-HSA-194121]. Reason: The pathway assertion matches the experimental literature on NTCP location and mechanism [PMID:22029531, PMID:35545671]. The basolateral child term is separately annotated and is the more informative location. Supporting Evidence: Reactome:R-HSA-194121 A molecule of extracellular bile salt (glyco- or taurocholate, or glyco- or taurochenodeoxycholate) and two sodium ions are transported into the cytosol, mediated by NTCP (Na+ / taurocholate cotransporter) in the plasma membrane. PMID:22029531 FRET (fluorescence resonance energy transfer) using fluorescently labelled subunits further demonstrated that dimerization persists at the plasma membrane. |
| GO:0008508 bile acid:sodium symporter activity | IBA GO_REF:0000033 | ACCEPT | Summary: Bile acid:sodium symporter activity propagated from the ancestral SLC10 bile-acid-transporter node, seeded by the experimentally characterized rat Ntcp and Asbt orthologs [GO_REF:0000033]. Reason: This is the core molecular function of SLC10A1 and the node placement is correct: human NTCP is strictly Na+-dependent and the cryo-EM transport cycle shows a bile-salt pore coupled to sodium sites [PMID:35545671]. Human-specific experimental evidence is also direct (Na+-dependent taurocholate uptake with Km ~6 uM in oocytes [PMID:8132774]). Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000040761 SUPPORTS TRANSFER Ancestral node for the Na+-dependent bile acid cotransporter clade; the symport activity is experimentally established in both human NTCP and the rodent orthologs used to place the IBD. Supporting Evidence: PMID:8132774 Injection of in vitro transcribed cRNA into Xenopus laevis oocytes resulted in the expression of Na(+)-dependent taurocholate uptake. PMID:8132774 Saturation kinetics indicated that the human NTCP has a higher affinity for taurocholate (apparent Km = 6 microM) than the previously cloned rat protein (apparent Km = 25 microM). PMID:35545671 NTCP undergoes a conformational transition opening a wide transmembrane pore that serves as the transport pathway for bile salts, and exposes key determinant residues for HBV/HDV binding to the outside of the cell. |
| GO:0008508 bile acid:sodium symporter activity | IEA GO_REF:0000107 | ACCEPT | Summary: Ensembl Compara transfer of the rat Ntcp (UniProtKB:P26435) bile acid:sodium symporter annotation to human SLC10A1. Reason: The orthology is one-to-one and the activity is independently demonstrated for the human protein [PMID:8132774, PMID:35545671], so the transferred term is correct even though it adds nothing beyond the direct human evidence. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:P26435 · Slc10a1 SUPPORTS TRANSFER Rat Ntcp, the originally cloned Na+/taurocholate cotransporter and a true ortholog of human SLC10A1. Supporting Evidence: PMID:8132774 Injection of in vitro transcribed cRNA into Xenopus laevis oocytes resulted in the expression of Na(+)-dependent taurocholate uptake. PMID:35545671 NTCP undergoes a conformational transition opening a wide transmembrane pore that serves as the transport pathway for bile salts, and exposes key determinant residues for HBV/HDV binding to the outside of the cell. |
| GO:0008508 bile acid:sodium symporter activity | TAS PMID:8132774 Molecular cloning, chromosomal localization, and functional ... | ACCEPT | Summary: The human NTCP cloning paper reports Na+-dependent, saturable taurocholate uptake (apparent Km ~6 uM) on expression in Xenopus oocytes [PMID:8132774]. Reason: This is the defining experiment for the gene's core activity, and sodium coupling has since been resolved structurally [PMID:35545671]. The term is at the right level of specificity for a Na+-coupled bile acid symporter. Supporting Evidence: PMID:8132774 Injection of in vitro transcribed cRNA into Xenopus laevis oocytes resulted in the expression of Na(+)-dependent taurocholate uptake. PMID:8132774 Saturation kinetics indicated that the human NTCP has a higher affinity for taurocholate (apparent Km = 6 microM) than the previously cloned rat protein (apparent Km = 25 microM). PMID:35545671 NTCP undergoes a conformational transition opening a wide transmembrane pore that serves as the transport pathway for bile salts, and exposes key determinant residues for HBV/HDV binding to the outside of the cell. |
| GO:0008508 bile acid:sodium symporter activity | TAS Reactome:R-HSA-194121 | ACCEPT | Summary: Reactome reaction asserting co-transport of a bile salt with two sodium ions into the hepatocyte cytosol by NTCP [Reactome:R-HSA-194121]. Reason: The stoichiometry and direction in the Reactome reaction match the experimentally and structurally characterized symport mechanism of human NTCP [PMID:8132774, PMID:35545671]; this is the gene's core molecular function. Supporting Evidence: Reactome:R-HSA-194121 A molecule of extracellular bile salt (glyco- or taurocholate, or glyco- or taurochenodeoxycholate) and two sodium ions are transported into the cytosol, mediated by NTCP (Na+ / taurocholate cotransporter) in the plasma membrane. PMID:35545671 NTCP undergoes a conformational transition opening a wide transmembrane pore that serves as the transport pathway for bile salts, and exposes key determinant residues for HBV/HDV binding to the outside of the cell. |
| GO:0015125 bile acid transmembrane transporter activity | IDA PMID:22029531 Homo- and hetero-dimeric architecture of the human liver Na⁺... | ACCEPT | Summary: NTCP-mediated taurocholate uptake was measured directly in NTCP-expressing cells, and reduced plasma-membrane NTCP abundance reduced that uptake [PMID:22029531]. Reason: Bile acid transmembrane transporter activity is directly supported, and the study also establishes that individual subunits of the NTCP dimer are functional [PMID:22029531]. The more specific sibling GO:0008508 (bile acid:sodium symporter activity) is separately annotated and captures the sodium coupling; keeping this parent term alongside it is appropriate for an IDA that measured transport without resolving the coupling ion. Supporting Evidence: PMID:22029531 Expression of SLC10A4 and NTCP Y307X resulted in a reduction of NTCP abundance at the plasma membrane and NTCP-mediated taurocholate uptake PMID:22029531 In conclusion, NTCP adopts a dimeric structure in which individual subunits are functional. |
| GO:0015125 bile acid transmembrane transporter activity | IEA GO_REF:0000107 | ACCEPT | Summary: Ensembl Compara transfer of bile acid transmembrane transporter activity from rat Ntcp (UniProtKB:P26435). Reason: Correct orthology-based transfer of an activity that is independently established for the human protein [PMID:8132774, PMID:14660639]. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:P26435 · Slc10a1 SUPPORTS TRANSFER Rat ortholog with direct bile acid transport assays. Supporting Evidence: PMID:8132774 Injection of in vitro transcribed cRNA into Xenopus laevis oocytes resulted in the expression of Na(+)-dependent taurocholate uptake. PMID:14660639 The key transporter responsible for hepatic uptake of bile acids from portal circulation is Na+-taurocholate cotransporting polypeptide (NTCP, SLC10A1). |
| GO:0015721 bile acid and bile salt transport | IBA GO_REF:0000033 | ACCEPT | Summary: Bile acid and bile salt transport propagated from the ancestral SLC10 bile acid transporter node [GO_REF:0000033]. SLC10A1 performs the hepatocyte uptake step of enterohepatic bile salt cycling [PMID:24867799, PMID:33222321]. Reason: SLC10A1 itself executes a transport step of this process — it is the carrier that moves conjugated bile salts from portal blood into the hepatocyte [PMID:24867799, PMID:34060352] — so the participation requirement is met directly. Human loss of function produces extreme plasma hypercholanemia, confirming that this step depends on SLC10A1 [PMID:24867799]. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN000040761 SUPPORTS TRANSFER Ancestral bile acid cotransporter node; bile salt transport is the defining shared function of the NTCP/ASBT clade. Supporting Evidence: PMID:24867799 The Na(+)-taurocholate cotransporting polypeptide SLC10A1 (NTCP) plays a key role in this process as the major transporter of conjugated bile salts from the plasma compartment into the hepatocyte. PMID:34060352 The Na+/taurocholate cotransporting polypeptide (NTCP) is located in the basolateral membrane of hepatocytes, where it transports bile acids from the portal blood back into hepatocytes. PMID:24867799 Total bile salts in plasma were extremely elevated (up to 1,500 |
| GO:0015721 bile acid and bile salt transport | IEA GO_REF:0000107 | ACCEPT | Summary: Ensembl Compara transfer of bile acid and bile salt transport from rat Ntcp (UniProtKB:P26435). Reason: Correct transfer between true orthologs for a process the human protein demonstrably carries out [PMID:24867799, PMID:35545671]. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:P26435 · Slc10a1 SUPPORTS TRANSFER Rat Ntcp; hepatic bile salt uptake is its characterized function. Supporting Evidence: PMID:24867799 The Na(+)-taurocholate cotransporting polypeptide SLC10A1 (NTCP) plays a key role in this process as the major transporter of conjugated bile salts from the plasma compartment into the hepatocyte. PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. |
| GO:0015721 bile acid and bile salt transport | TAS Reactome:R-HSA-159418 | ACCEPT | Summary: Reactome places SLC10A1 as the hepatocyte uptake transporter in 'Recycling of bile acids and salts' [Reactome:R-HSA-159418]. Reason: This is the correct process assignment and the correct role within it: NTCP performs the basolateral uptake step of enterohepatic cycling [Reactome:R-HSA-159418, PMID:33222321]. Supporting Evidence: Reactome:R-HSA-159418 uptake by hepatocytes mediated by Na+-taurocholate transporting protein (NTPC - SLC10A1) PMID:33222321 The most prominent biochemical feature was the extremely elevated total bile salt level in plasma (up to 1,500 |
| GO:0016020 membrane | IEA GO_REF:0000120 | ACCEPT | Summary: Automated InterPro/ARBA assignment of the generic term membrane from the bile acid:Na+ symporter domain signature (IPR002657). Reason: Not wrong, but much less informative than the plasma-membrane and basolateral plasma-membrane terms already annotated from direct evidence [PMID:22029531, PMID:34060352]. Broad IEA parents of correctly annotated children are acceptable, so this is retained rather than modified. Supporting Evidence: PMID:22029531 FRET (fluorescence resonance energy transfer) using fluorescently labelled subunits further demonstrated that dimerization persists at the plasma membrane. PMID:34060352 The Na+/taurocholate cotransporting polypeptide (NTCP) is located in the basolateral membrane of hepatocytes, where it transports bile acids from the portal blood back into hepatocytes. |
| GO:0016323 basolateral plasma membrane | IBA GO_REF:0000033 | ACCEPT | Summary: Basolateral plasma membrane activity propagated from a PAINT node within the SLC10 family (PANTHER:PTN002570905), consistent with NTCP's hepatocyte sinusoidal localization [PMID:34060352]. Reason: This is the most informative and biologically correct location for SLC10A1: NTCP sits in the basolateral (sinusoidal) membrane of hepatocytes facing portal blood, where it takes up bile acids [PMID:34060352, PMID:14660639]. The node placement is appropriate because the NTCP sub-branch (as distinct from the apical ileal ASBT branch) is basolateral. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN002570905 SUPPORTS TRANSFER NTCP-specific sub-node; basolateral membrane localization is the experimentally supported location for this branch, in contrast to the apical localization of the ileal ASBT branch. Supporting Evidence: PMID:34060352 The Na+/taurocholate cotransporting polypeptide (NTCP) is located in the basolateral membrane of hepatocytes, where it transports bile acids from the portal blood back into hepatocytes. PMID:14660639 The key transporter responsible for hepatic uptake of bile acids from portal circulation is Na+-taurocholate cotransporting polypeptide (NTCP, SLC10A1). |
| GO:0016323 basolateral plasma membrane | IEA GO_REF:0000107 | ACCEPT | Summary: Ensembl Compara transfer of basolateral plasma membrane localization from rat Ntcp (UniProtKB:P26435). Reason: The localization is conserved and directly reported for human NTCP in hepatocytes [PMID:34060352]; human disease variants act by failing to reach the plasma membrane [PMID:24867799]. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:P26435 · Slc10a1 SUPPORTS TRANSFER Rat Ntcp is basolateral in hepatocytes, as is the human protein. Supporting Evidence: PMID:34060352 The Na+/taurocholate cotransporting polypeptide (NTCP) is located in the basolateral membrane of hepatocytes, where it transports bile acids from the portal blood back into hepatocytes. PMID:24867799 Immunofluorescence studies and surface biotinylation experiments demonstrated that the mutant protein is virtually absent from the plasma membrane. |
| GO:0016323 basolateral plasma membrane | ISS GO_REF:0000024 | ACCEPT | Summary: Curator-judged sequence-similarity transfer of basolateral plasma membrane localization from rat Ntcp (UniProtKB:P26435). Reason: Same conclusion as the Compara transfer and consistent with direct human data [PMID:34060352]; the curator's orthology judgment is correct for this one-to-one ortholog pair. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:P26435 · Slc10a1 SUPPORTS TRANSFER Rat ortholog with established basolateral hepatocyte localization. Supporting Evidence: PMID:34060352 The Na+/taurocholate cotransporting polypeptide (NTCP) is located in the basolateral membrane of hepatocytes, where it transports bile acids from the portal blood back into hepatocytes. |
| GO:0031667 response to nutrient levels | IEA GO_REF:0000107 | REMOVE | Summary: Response to nutrient levels, transferred automatically from rat Ntcp (UniProtKB:P26435). The donor annotation reflects changes in rat Ntcp expression/activity under altered nutritional states, not a function SLC10A1 executes within that response. Reason: This is an expression-response annotation propagated as process participation. SLC10A1 is the transporter whose abundance is modulated; it does none of the work of sensing or responding to nutrient levels, and no step of that response is catalysed or structurally enabled by NTCP. The gene's characterized activity is Na+-coupled bile salt uptake [PMID:8132774, PMID:35545671], and the human literature describes no nutrient-sensing role; indeed NTCP deficiency leaves bile salt synthesis and intestinal bile salt signalling intact [PMID:24867799]. 'Being regulated by X' is not 'involved in the response to X'. Propagation Review Root cause: PROPAGATION BAD Failure modes: ROLE CONFLATION Sources checked: UniProtKB:P26435 · Slc10a1 SUPPORTS SOURCE BUT NOT TARGET Donor rat annotation records transcriptional/functional modulation of Ntcp under altered nutrient states rather than participation of the transporter in a nutrient response pathway. Supporting Evidence: PMID:35545671 Human Na+-taurocholate co-transporting polypeptide (NTCP) is the main bile salt uptake system in liver. PMID:24867799 The Na(+)-taurocholate cotransporting polypeptide SLC10A1 (NTCP) plays a key role in this process as the major transporter of conjugated bile salts from the plasma compartment into the hepatocyte. |
| GO:0043627 response to estrogen | IEA GO_REF:0000107 | REMOVE | Summary: Response to estrogen, transferred automatically from rat Ntcp (UniProtKB:P26435). Rat Ntcp expression is down-regulated by estrogens, and human NTCP transports the estrogen conjugate estrone-3-sulfate [PMID:14660639]. Reason: Two distinct facts underlie this term and neither licenses it. Being transcriptionally repressed by estrogen is regulation of the gene, not participation by the gene product in an estrogen response. Transporting estrone-3-sulfate [PMID:14660639] is a substrate relationship whose correct GO representation is a transporter-activity term (already covered by the bile acid/organic anion transport annotations), not a 'response to estrogen' process term. Propagation Review Root cause: PROPAGATION BAD Failure modes: ROLE CONFLATION Sources checked: UniProtKB:P26435 · Slc10a1 SUPPORTS SOURCE BUT NOT TARGET Donor annotation reflects hormonal regulation of rat Ntcp expression, not a function Ntcp performs in responding to estrogen. Supporting Evidence: PMID:14660639 exhibited a near complete loss of function for bile acid uptake yet fully normal transport function for the non-bile acid substrate estrone sulfate |
| GO:0045471 response to ethanol | IEA GO_REF:0000107 | REMOVE | Summary: Response to ethanol, transferred automatically from rat Ntcp (UniProtKB:P26435), where the underlying observation is altered Ntcp expression/transport in ethanol-treated animals. Reason: SLC10A1 performs no step of an ethanol response: it neither metabolizes ethanol nor transports it. The donor annotation records that the transporter is affected by ethanol exposure, which is regulation of the gene product rather than participation. No human evidence connects NTCP to ethanol handling; its characterized substrates are conjugated and unconjugated bile salts and sulfated steroids [PMID:14660639, PMID:9458785]. Propagation Review Root cause: PROPAGATION BAD Failure modes: ROLE CONFLATION Sources checked: UniProtKB:P26435 · Slc10a1 SUPPORTS SOURCE BUT NOT TARGET Donor rat annotation captures an expression change on ethanol exposure, not an activity of Ntcp within an ethanol response. Supporting Evidence: PMID:14660639 exhibited a near complete loss of function for bile acid uptake yet fully normal transport function for the non-bile acid substrate estrone sulfate PMID:9458785 Whereas the multispecific liver Na(+)-bile acid cotransporter may participate in hepatic clearance of organic anion metabolites and xenobiotics, the ileal and renal Na(+)-bile acid cotransporter retains a narrow specificity for reclamation of bile acids. |
| GO:0071466 cellular response to xenobiotic stimulus | IEA GO_REF:0000107 | MODIFY | Summary: Cellular response to xenobiotic stimulus, transferred from rat Ntcp (UniProtKB:P26435). Human NTCP is a multispecific carrier that takes up drugs and other organic anions in addition to bile salts [PMID:9458785, PMID:34060352]. Reason: Unlike the nutrient/estrogen/ethanol rows, there is a real transport function here rather than only a regulatory effect: the liver Na+-bile acid cotransporter is explicitly described as multispecific and as participating in hepatic clearance of organic anion metabolites and xenobiotics [PMID:9458785], and human NTCP transports drugs such as rosuvastatin [PMID:34060352]. But the function NTCP executes is uptake of the xenobiotic, not a cellular response to it, so the process term is the wrong kind. Replace with the transport process term that describes what the protein actually does. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: ROLE CONFLATION Sources checked: UniProtKB:P26435 · Slc10a1 SUPPORTS SOURCE BUT NOT TARGET Donor annotation frames xenobiotic handling as a cellular response rather than as the transport activity the carrier performs. Proposed replacements: xenobiotic transport Supporting Evidence: PMID:9458785 Whereas the multispecific liver Na(+)-bile acid cotransporter may participate in hepatic clearance of organic anion metabolites and xenobiotics, the ileal and renal Na(+)-bile acid cotransporter retains a narrow specificity for reclamation of bile acids. PMID:34060352 258 ± 42 µM and 199 ± 13 µM for the drug rosuvastatin |
| GO:0001618 virus receptor activity | IDA PMID:23150796 Sodium taurocholate cotransporting polypeptide is a function... | NEW | Summary: Human NTCP is the cellular entry receptor for hepatitis B virus and its satellite hepatitis D virus: the preS1 receptor-binding region of the large envelope protein cross-links specifically to NTCP, silencing NTCP blocks infection, and ectopic NTCP renders non-susceptible hepatoma cells susceptible [PMID:23150796]. Cryo-EM structures show the preS1 determinant residues exposed on the outward-open transporter [PMID:35545671]. Reason: This is a well-established, highly specific molecular function of the human protein that is absent from the current GOA set, which contains only transport and localization terms. NTCP itself does the work of the receptor step: it binds the viral preS1 domain directly (photo-cross-linking and structural evidence) and its presence is both necessary and sufficient for susceptibility in cell culture [PMID:23150796]. UniProt curates the same function as a (microbial infection) FUNCTION block. Comparator check: other non-immune host proteins serving as entry receptors alongside their own enzymatic or transport function carry this term by IDA, e.g. ACE2 (UniProtKB:Q9BYF1) and CD4 (UniProtKB:P01730), so the term is conventionally applied to exactly this role. Supporting Evidence: PMID:23150796 Silencing NTCP inhibited HBV and HDV infection, while exogenous NTCP expression rendered nonsusceptible hepatocarcinoma cells susceptible to these viral infections. PMID:35545671 NTCP undergoes a conformational transition opening a wide transmembrane pore that serves as the transport pathway for bile salts, and exposes key determinant residues for HBV/HDV binding to the outside of the cell. |
| GO:0046718 symbiont entry into host cell | IDA PMID:23150796 Sodium taurocholate cotransporting polypeptide is a function... | NEW | Summary: By acting as the preS1-binding entry receptor, NTCP mediates entry of HBV and HDV into hepatocytes; NTCP knockdown abolishes and NTCP expression confers infection [PMID:23150796]. Humanizing residues 157-165 of the non-functional monkey orthologue is sufficient to support both viral infections [PMID:23150796]. Reason: Participation test: NTCP performs a step of viral entry rather than merely being required for it — it is the surface receptor that engages the viral envelope determinant, and the conformational state of the transporter governs preS1 recognition [PMID:35545671]. Comparator check: ACE2 (UniProtKB:Q9BYF1) and CD4 (UniProtKB:P01730) both carry GO:0046718 for the same receptor role, so this is the conventional process term for host entry receptors rather than an idiosyncratic addition. Proposed as non-core relative to bile salt uptake: it is a host-pathogen interaction exploited by the virus, not the physiological role for which the transporter evolved. Supporting Evidence: PMID:23150796 Silencing NTCP inhibited HBV and HDV infection, while exogenous NTCP expression rendered nonsusceptible hepatocarcinoma cells susceptible to these viral infections. PMID:35545671 NTCP undergoes a conformational transition opening a wide transmembrane pore that serves as the transport pathway for bile salts, and exposes key determinant residues for HBV/HDV binding to the outside of the cell. |
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Download this section (compressed HTML)Q: Should NTCP-mediated uptake of non-bile-acid substrates (estrone-3-sulfate, rosuvastatin) be captured as a distinct molecular function annotation, and if so with which transporter-activity term, now that the generic organic anion transport terms have been obsoleted?
Q: Does GO wish to record substrate scope for multispecific carriers via has_input extensions on the transporter activity annotation, rather than through process terms such as cellular response to xenobiotic stimulus?
Q: NTCP forms homodimers and heteromers with SLC10A4/SLC10A6 that modulate its surface delivery [PMID:22029531]. Is there sufficient evidence for a protein-containing complex annotation, given that individual subunits are reported to be independently functional?
Experiment: Quantitative substrate profiling of purified human NTCP reconstituted in proteoliposomes to delimit the physiological substrate range (bile salts vs sulfated steroids vs drugs) and the sodium stoichiometry for each substrate class.
Experiment: Structure-guided separation-of-function mutants that abolish preS1 binding while retaining bile salt symport (and vice versa), to test whether the receptor and transport functions can be uncoupled and to support distinct GO annotations for each.
Experiment: Hepatocyte-specific conditional Slc10a1 models with bile salt flux measurements under defined nutritional and hormonal states, to determine whether the reported nutrient-, estrogen- and ethanol-responsiveness of the transporter reflects anything beyond regulation of its expression.
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