SLC16A1 encodes monocarboxylate transporter 1 (MCT1), a 12-transmembrane major facilitator superfamily carrier that catalyses bidirectional, proton-coupled transport of monocarboxylates across the plasma membrane, chiefly L-lactate and pyruvate but also acetate, the ketone bodies acetoacetate and beta-hydroxybutyrate, branched-chain 2-oxo acids and, at low pH, monoprotonated succinate. Direction is set by the substrate and proton gradients, so MCT1 serves as a lactate exporter in glycolytic cells and an importer in oxidative tissues, contributing to the Cori cycle, lactate shuttles and intracellular pH. Delivery to and activity at the cell surface require the ancillary glycoprotein basigin (BSG/CD147) or embigin. MCT1 is widely expressed, with basolateral and apical pools in polarised epithelia, but is transcriptionally silenced ("disallowed") in pancreatic beta cells; promoter variants that de-repress it there allow pyruvate and lactate to stimulate insulin secretion, causing exercise-induced hyperinsulinism (HHF7). Loss-of-function variants cause MCT1 deficiency with recurrent ketoacidosis.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005515 protein binding | IPI PMID:10921872 CD147 is tightly associated with lactate transporters MCT1 a... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:36115835 Quantitative fragmentomics allow affinity mapping of interac... | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005515 protein binding | IPI PMID:40355756 The solute carrier superfamily interactome. | REMOVE | Summary: Bare protein binding from interaction data (BSG/CD147 chaperone or high-throughput PDZ/SLC interactome screens). Reason: Protein binding conveys no functional information. The interactions (e.g. the BSG ancillary-protein interaction needed for surface expression, PDZ-domain fragment binding) may well be real, but removal of this uninformative term does not mean the interaction is false; the functionally relevant BSG association is captured in core function descriptions. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005813 centrosome | IDA PMID:23816619 The centriolar satellite protein SSX2IP promotes centrosome ... | UNDECIDED | Summary: Centrosome localization from a proteomic survey of spindle/centrosome proteins (SSX2IP study). Reason: The cached full text of PMID:23816619 (PMC3704989, about SSX2IP) contains no mention of MCT1, SLC16A1 or monocarboxylate transport, so the supporting observation is presumably in supplementary data that is not cached and could not be checked. A centrosomal location would be unexpected for a 12-TM plasma-membrane MFS carrier, and nothing elsewhere supports it, but an experimental annotation is not removed on that basis alone. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates |
| GO:0005886 plasma membrane | EXP PMID:10921872 CD147 is tightly associated with lactate transporters MCT1 a... | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | EXP PMID:12946269 The loop between helix 4 and helix 5 in the monocarboxylate ... | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | EXP PMID:17127621 The role of charged residues in the transmembrane helices of... | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | IBA GO_REF:0000033 | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | IDA GO_REF:0000052 | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | IDA PMID:15505343 Presence and localization of three lactic acid transporters ... | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | IDA PMID:23137377 Quantitative targeted absolute proteomic analysis of transpo... | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | IDA PMID:24390345 Sequence variants in SLC16A11 are a common risk factor for t... | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | IDA PMID:25371203 Functional characterization of 5-oxoproline transport via SL... | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | IDA PMID:25957687 Rod-derived cone viability factor promotes cone survival by ... | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | IDA PMID:28112518 LC-MS/MS Based Quantitation of ABC and SLC Transporter Prote... | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | IEA GO_REF:0000120 | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | ISS GO_REF:0000024 | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | ISS GO_REF:0000024 | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-204392 | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-373867 | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-373875 | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-5624211 | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0005886 plasma membrane | TAS Reactome:R-HSA-9645220 | ACCEPT | Summary: Plasma membrane localization of MCT1, the site where it carries out proton-coupled monocarboxylate transport. Reason: MCT1 is a multi-pass plasma membrane transporter; surface delivery requires BSG/EMB. Consistent with many independent experimental studies. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0006629 lipid metabolic process | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Lipid metabolic process (Ensembl Compara electronic transfer). Reason: MCT1 transports ketone bodies and short-chain fatty acids but performs no step of lipid metabolism; the term over-reaches from transport to metabolism. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate |
| GO:0007098 centrosome cycle | IMP PMID:23816619 The centriolar satellite protein SSX2IP promotes centrosome ... | UNDECIDED | Summary: Centrosome cycle (IMP) from the SSX2IP centrosome maturation study. Reason: The cached full text of PMID:23816619 (PMC3704989, about SSX2IP) contains no mention of MCT1, SLC16A1 or monocarboxylate transport, so the phenotype behind this IMP is presumably in supplementary screen data that is not cached and could not be checked. No mechanism is known by which a plasma-membrane monocarboxylate transporter would act in the centrosome cycle, but an experimental annotation is not removed on that basis alone. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Bidirectional proton-coupled monocarboxylate transporter |
| GO:0008028 monocarboxylic acid transmembrane transporter activity | IDA PMID:25371203 Functional characterization of 5-oxoproline transport via SL... | ACCEPT | Summary: Monocarboxylic acid transmembrane transporter activity. Reason: Core activity: MCT1 transports lactate, pyruvate, acetate and ketone bodies across the plasma membrane with a proton. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Bidirectional proton-coupled monocarboxylate transporter file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate PMID:8124722 Lactate and pyruvate cross cell membranes via a monocarboxylate transporter |
| GO:0008028 monocarboxylic acid transmembrane transporter activity | IEA GO_REF:0000120 | ACCEPT | Summary: Monocarboxylic acid transmembrane transporter activity. Reason: Core activity: MCT1 transports lactate, pyruvate, acetate and ketone bodies across the plasma membrane with a proton. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Bidirectional proton-coupled monocarboxylate transporter file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate PMID:8124722 Lactate and pyruvate cross cell membranes via a monocarboxylate transporter |
| GO:0008028 monocarboxylic acid transmembrane transporter activity | ISS GO_REF:0000024 | ACCEPT | Summary: Monocarboxylic acid transmembrane transporter activity. Reason: Core activity: MCT1 transports lactate, pyruvate, acetate and ketone bodies across the plasma membrane with a proton. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Bidirectional proton-coupled monocarboxylate transporter file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate PMID:8124722 Lactate and pyruvate cross cell membranes via a monocarboxylate transporter |
| GO:0008028 monocarboxylic acid transmembrane transporter activity | TAS PMID:8124722 Molecular characterization of a membrane transporter for lac... | ACCEPT | Summary: Monocarboxylic acid transmembrane transporter activity. Reason: Core activity: MCT1 transports lactate, pyruvate, acetate and ketone bodies across the plasma membrane with a proton. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Bidirectional proton-coupled monocarboxylate transporter file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate PMID:8124722 Lactate and pyruvate cross cell membranes via a monocarboxylate transporter |
| GO:0009925 basal plasma membrane | IDA PMID:26854723 Butyric acid increases transepithelial transport of ferulic ... | KEEP AS NON CORE | Summary: Basal plasma membrane from the Caco-2 butyrate study. Reason: Consistent with the basolateral localisation; a plasma membrane sub-domain and not separately core. Supporting Evidence: PMID:26854723 Immunofluorescence staining of cells revealed uniform |
| GO:0015129 lactate transmembrane transporter activity | IEA GO_REF:0000120 | ACCEPT | Summary: Lactate transmembrane transporter activity. Reason: L-lactate is the best-characterised MCT1 substrate (Km ~5 mM); MCT1 is a major lactate exporter. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate file:human/SLC16A1/SLC16A1-uniprot.txt major lactate exporter (By similarity) |
| GO:0015129 lactate transmembrane transporter activity | ISS GO_REF:0000024 | ACCEPT | Summary: Lactate transmembrane transporter activity. Reason: L-lactate is the best-characterised MCT1 substrate (Km ~5 mM); MCT1 is a major lactate exporter. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate file:human/SLC16A1/SLC16A1-uniprot.txt major lactate exporter (By similarity) |
| GO:0015129 lactate transmembrane transporter activity | TAS PMID:25371203 Functional characterization of 5-oxoproline transport via SL... | ACCEPT | Summary: Lactate transmembrane transporter activity. Reason: L-lactate is the best-characterised MCT1 substrate (Km ~5 mM); MCT1 is a major lactate exporter. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate file:human/SLC16A1/SLC16A1-uniprot.txt major lactate exporter (By similarity) |
| GO:0015130 mevalonate transmembrane transporter activity | TAS PMID:1429658 cDNA cloning of MEV, a mutant protein that facilitates cellu... | KEEP AS NON CORE | Summary: Mevalonate transmembrane transporter activity (TAS, MEV cloning paper). Reason: The MEV paper describes a Phe360Cys gain-of-function MCT1 mutant (MEV) that transports mevalonate; wild-type MCT1 is not a physiological mevalonate transporter. Kept as non-core rather than removed because the curator's source reports the activity for this protein (mutant form). Supporting Evidence: PMID:1429658 cDNA cloning of MEV, a mutant protein that facilitates cellular uptake of PMID:8124722 A Phe to Cys |
| GO:0015141 succinate transmembrane transporter activity | IDA PMID:32946811 pH-Gated Succinate Secretion Regulates Muscle Remodeling in ... | ACCEPT | Summary: Succinate transmembrane transporter activity (IDA). Reason: In acidified exercising muscle, succinate becomes monoprotonated and is exported by MCT1 (pH-gated succinate secretion). Supporting Evidence: PMID:32946811 succinate is rendered a transport substrate for monocarboxylate file:human/SLC16A1/SLC16A1-uniprot.txt Facilitates the protonated monocarboxylate form of succinate export, |
| GO:0015293 symporter activity | IEA GO_REF:0000117 | ACCEPT | Summary: Symporter activity (UniRule). Reason: Correct general mechanism (H+-coupled symport); less specific than lactate:proton symporter. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Bidirectional proton-coupled monocarboxylate transporter |
| GO:0015295 solute:proton symporter activity | IEA GO_REF:0000107 | ACCEPT | Summary: Solute:proton symporter activity. Reason: Correct; MCT1 is a proton-coupled solute symporter. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Bidirectional proton-coupled monocarboxylate transporter |
| GO:0015355 secondary active monocarboxylate transmembrane transporter activity | IEA GO_REF:0000117 | ACCEPT | Summary: Secondary active monocarboxylate transmembrane transporter activity. Reason: Correct parent of the proton-coupled monocarboxylate symport; accepted (lactate:proton symporter is more specific). Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Bidirectional proton-coupled monocarboxylate transporter |
| GO:0015650 lactate:proton symporter activity | IBA GO_REF:0000033 | ACCEPT | Summary: Lactate:proton symporter activity, the most precise description of MCT1 transport mechanism. Reason: MCT1 cotransports L-lactate with H+ (Rhea:29415); proton symport is the defining MCT mechanism. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Bidirectional proton-coupled monocarboxylate transporter PMID:8124722 properties resemble those of the erythrocyte MCT, including proton symport |
| GO:0015650 lactate:proton symporter activity | IDA PMID:12946269 The loop between helix 4 and helix 5 in the monocarboxylate ... | ACCEPT | Summary: Lactate:proton symporter activity, the most precise description of MCT1 transport mechanism. Reason: MCT1 cotransports L-lactate with H+ (Rhea:29415); proton symport is the defining MCT mechanism. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Bidirectional proton-coupled monocarboxylate transporter PMID:8124722 properties resemble those of the erythrocyte MCT, including proton symport |
| GO:0015650 lactate:proton symporter activity | IEA GO_REF:0000107 | ACCEPT | Summary: Lactate:proton symporter activity, the most precise description of MCT1 transport mechanism. Reason: MCT1 cotransports L-lactate with H+ (Rhea:29415); proton symport is the defining MCT mechanism. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Bidirectional proton-coupled monocarboxylate transporter PMID:8124722 properties resemble those of the erythrocyte MCT, including proton symport |
| GO:0015718 monocarboxylic acid transport | IDA PMID:25371203 Functional characterization of 5-oxoproline transport via SL... | ACCEPT | Summary: Monocarboxylic acid transport. Reason: Core process carried out by MCT1. Supporting Evidence: PMID:8124722 Lactate and pyruvate cross cell membranes via a monocarboxylate transporter file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate |
| GO:0015718 monocarboxylic acid transport | IEA GO_REF:0000120 | ACCEPT | Summary: Monocarboxylic acid transport. Reason: Core process carried out by MCT1. Supporting Evidence: PMID:8124722 Lactate and pyruvate cross cell membranes via a monocarboxylate transporter file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate |
| GO:0015718 monocarboxylic acid transport | ISS GO_REF:0000024 | ACCEPT | Summary: Monocarboxylic acid transport. Reason: Core process carried out by MCT1. Supporting Evidence: PMID:8124722 Lactate and pyruvate cross cell membranes via a monocarboxylate transporter file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate |
| GO:0015718 monocarboxylic acid transport | TAS PMID:8124722 Molecular characterization of a membrane transporter for lac... | ACCEPT | Summary: Monocarboxylic acid transport. Reason: Core process carried out by MCT1. Supporting Evidence: PMID:8124722 Lactate and pyruvate cross cell membranes via a monocarboxylate transporter file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate |
| GO:0015728 mevalonate transport | TAS PMID:1429658 cDNA cloning of MEV, a mutant protein that facilitates cellu... | KEEP AS NON CORE | Summary: Mevalonate transport (TAS). Reason: As for mevalonate transporter activity: a property of the MEV gain-of-function mutant, not a physiological wild-type process. Supporting Evidence: PMID:1429658 cDNA cloning of MEV, a mutant protein that facilitates cellular uptake of |
| GO:0016020 membrane | HDA PMID:19946888 Defining the membrane proteome of NK cells. | ACCEPT | Summary: Generic membrane localization. Reason: Correct but uninformative; MCT1 is an integral plasma membrane protein. Accepted as a non-controversial general location. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates |
| GO:0016020 membrane | IEA GO_REF:0000002 | ACCEPT | Summary: Generic membrane localization. Reason: Correct but uninformative; MCT1 is an integral plasma membrane protein. Accepted as a non-controversial general location. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates |
| GO:0016020 membrane | TAS PMID:1429658 cDNA cloning of MEV, a mutant protein that facilitates cellu... | ACCEPT | Summary: Generic membrane localization. Reason: Correct but uninformative; MCT1 is an integral plasma membrane protein. Accepted as a non-controversial general location. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates |
| GO:0016020 membrane | TAS PMID:8124722 Molecular characterization of a membrane transporter for lac... | ACCEPT | Summary: Generic membrane localization. Reason: Correct but uninformative; MCT1 is an integral plasma membrane protein. Accepted as a non-controversial general location. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates |
| GO:0016323 basolateral plasma membrane | IBA GO_REF:0000033 | ACCEPT | Summary: Basolateral plasma membrane localization (e.g. intestinal and retinal pigment epithelium, blood-brain barrier). Reason: MCT1 localises to the basolateral membrane of polarised epithelia where it exports lactate, as in its originally described intestinal epithelial distribution. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Basolateral cell membrane PMID:8124722 MCT1 is abundant in erythrocytes, cardiac muscle, and basolateral intestinal epithelium |
| GO:0016323 basolateral plasma membrane | IEA GO_REF:0000120 | ACCEPT | Summary: Basolateral plasma membrane localization (e.g. intestinal and retinal pigment epithelium, blood-brain barrier). Reason: MCT1 localises to the basolateral membrane of polarised epithelia where it exports lactate, as in its originally described intestinal epithelial distribution. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Basolateral cell membrane PMID:8124722 MCT1 is abundant in erythrocytes, cardiac muscle, and basolateral intestinal epithelium |
| GO:0016323 basolateral plasma membrane | ISS GO_REF:0000024 | ACCEPT | Summary: Basolateral plasma membrane localization (e.g. intestinal and retinal pigment epithelium, blood-brain barrier). Reason: MCT1 localises to the basolateral membrane of polarised epithelia where it exports lactate, as in its originally described intestinal epithelial distribution. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Basolateral cell membrane PMID:8124722 MCT1 is abundant in erythrocytes, cardiac muscle, and basolateral intestinal epithelium |
| GO:0016323 basolateral plasma membrane | ISS PMID:18619525 Subcellular localization of transporters along the rat blood... | ACCEPT | Summary: Basolateral plasma membrane localization (e.g. intestinal and retinal pigment epithelium, blood-brain barrier). Reason: MCT1 localises to the basolateral membrane of polarised epithelia where it exports lactate, as in its originally described intestinal epithelial distribution. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Basolateral cell membrane PMID:8124722 MCT1 is abundant in erythrocytes, cardiac muscle, and basolateral intestinal epithelium |
| GO:0016324 apical plasma membrane | IDA PMID:15901598 Expression and membrane localization of MCT isoforms along t... | ACCEPT | Summary: Apical plasma membrane localization in polarised epithelia (intestine, RPE). Reason: Experimentally observed apical membrane pool in several epithelia; a valid but tissue-dependent location of the transporter. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Apical cell membrane |
| GO:0016324 apical plasma membrane | IDA PMID:26854723 Butyric acid increases transepithelial transport of ferulic ... | ACCEPT | Summary: Apical plasma membrane localization in polarised epithelia (intestine, RPE). Reason: Experimentally observed apical membrane pool in several epithelia; a valid but tissue-dependent location of the transporter. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Apical cell membrane |
| GO:0016324 apical plasma membrane | IDA PMID:31678436 The circadian clock regulates RPE-mediated lactate transport... | ACCEPT | Summary: Apical plasma membrane localization in polarised epithelia (intestine, RPE). Reason: Experimentally observed apical membrane pool in several epithelia; a valid but tissue-dependent location of the transporter. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Apical cell membrane |
| GO:0016324 apical plasma membrane | IDA PMID:31791063 Quantitative Protein Expression in the Human Retinal Pigment... | ACCEPT | Summary: Apical plasma membrane localization in polarised epithelia (intestine, RPE). Reason: Experimentally observed apical membrane pool in several epithelia; a valid but tissue-dependent location of the transporter. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Apical cell membrane |
| GO:0016324 apical plasma membrane | IEA GO_REF:0000120 | ACCEPT | Summary: Apical plasma membrane localization in polarised epithelia (intestine, RPE). Reason: Experimentally observed apical membrane pool in several epithelia; a valid but tissue-dependent location of the transporter. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Apical cell membrane |
| GO:0016324 apical plasma membrane | ISS PMID:18619525 Subcellular localization of transporters along the rat blood... | ACCEPT | Summary: Apical plasma membrane localization in polarised epithelia (intestine, RPE). Reason: Experimentally observed apical membrane pool in several epithelia; a valid but tissue-dependent location of the transporter. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Apical cell membrane |
| GO:0016328 lateral plasma membrane | IDA PMID:26854723 Butyric acid increases transepithelial transport of ferulic ... | KEEP AS NON CORE | Summary: Lateral plasma membrane staining in Caco-2 cells. Reason: Reported in a single Caco-2 study; a sub-domain of the plasma membrane location already accepted. Not core. Supporting Evidence: PMID:26854723 Immunofluorescence staining of cells revealed uniform |
| GO:0022857 transmembrane transporter activity | IEA GO_REF:0000002 | ACCEPT | Summary: Transmembrane transporter activity (InterPro). Reason: Correct but very general; superseded by the specific monocarboxylate terms. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Bidirectional proton-coupled monocarboxylate transporter |
| GO:0030054 cell junction | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: Cell junction localization from the Human Protein Atlas imaging. Reason: HPA immunofluorescence; MCT1/BSG concentrate at intercalated discs in heart cells, so junctional enrichment is plausible but not a core location. Supporting Evidence: PMID:10921872 CD147 and MCT1 co-localize, concentrating at the |
| GO:0032094 response to food | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Response to food (electronic from rodent studies). Reason: Organismal physiological response in rodents (e.g. high-fat diet); indirect and non-core. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt lactate and pyruvate that contribute to the regulation of central |
| GO:0035873 lactate transmembrane transport | IEA GO_REF:0000107 | ACCEPT | Summary: Lactate transmembrane transport. Reason: Core process. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate |
| GO:0035879 plasma membrane lactate transport | IBA GO_REF:0000033 | ACCEPT | Summary: Plasma membrane lactate transport. Reason: Most specific process term for MCT1 core function: lactate import/export across the plasma membrane. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt major lactate exporter (By similarity) |
| GO:0035879 plasma membrane lactate transport | IEA GO_REF:0000120 | ACCEPT | Summary: Plasma membrane lactate transport. Reason: Most specific process term for MCT1 core function: lactate import/export across the plasma membrane. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt major lactate exporter (By similarity) |
| GO:0035879 plasma membrane lactate transport | ISS GO_REF:0000024 | ACCEPT | Summary: Plasma membrane lactate transport. Reason: Most specific process term for MCT1 core function: lactate import/export across the plasma membrane. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt major lactate exporter (By similarity) |
| GO:0035879 plasma membrane lactate transport | ISS GO_REF:0000024 | ACCEPT | Summary: Plasma membrane lactate transport. Reason: Most specific process term for MCT1 core function: lactate import/export across the plasma membrane. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt major lactate exporter (By similarity) |
| GO:0035879 plasma membrane lactate transport | TAS PMID:25371203 Functional characterization of 5-oxoproline transport via SL... | ACCEPT | Summary: Plasma membrane lactate transport. Reason: Most specific process term for MCT1 core function: lactate import/export across the plasma membrane. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates file:human/SLC16A1/SLC16A1-uniprot.txt major lactate exporter (By similarity) |
| GO:0042593 glucose homeostasis | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Glucose homeostasis (electronic from mouse). Reason: Mouse haploinsufficiency studies link MCT1 to systemic glucose/insulin regulation under high-fat diet; indirect consequence of monocarboxylate transport. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt lactate and pyruvate that contribute to the regulation of central |
| GO:0042802 identical protein binding | IEA GO_REF:0000107 | ACCEPT | Summary: Identical protein binding (electronic). Reason: MCT1 forms homodimers in the BSG-MCT1 complex; low-information but not wrong. Accepted as non-core structural property. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Expression at the cell surface |
| GO:0042867 pyruvate catabolic process | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Pyruvate catabolic process (electronic from rodent). Reason: MCT1 delivers pyruvate into or out of cells but does not catalyse its catabolism; transport is upstream of catabolism. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate |
| GO:0045202 synapse | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Synapse localization transferred electronically from rodent data. Reason: MCT1 is expressed on neural/glial membranes in the brain (lactate shuttle); synaptic enrichment is not its defining location. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates |
| GO:0046943 carboxylic acid transmembrane transporter activity | IEA GO_REF:0000107 | ACCEPT | Summary: Carboxylic acid transmembrane transporter activity. Reason: Correct parent term for monocarboxylate transport. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate |
| GO:0046943 carboxylic acid transmembrane transporter activity | ISS GO_REF:0000024 | ACCEPT | Summary: Carboxylic acid transmembrane transporter activity. Reason: Correct parent term for monocarboxylate transport. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate |
| GO:0050796 regulation of insulin secretion | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Regulation of insulin secretion (electronic from mouse). Reason: MCT1 is normally a beta-cell "disallowed" gene; its expression in beta cells (as in HHF7) allows pyruvate/lactate to trigger insulin release. In normal physiology the effect on insulin secretion is indirect, via whole-body monocarboxylate fluxes. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt lactate and pyruvate that contribute to the regulation of central PMID:17701893 induce SLC16A1 expression in beta cells, where this gene is not usually transcribed, permitting pyruvate uptake and pyruvate-stimulated insulin release despite ensuing hypoglycemia |
| GO:0051780 behavioral response to nutrient | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Behavioral response to nutrient (electronic from rodent). Reason: Organism-level phenotype in rodent models; indirect. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt lactate and pyruvate that contribute to the regulation of central |
| GO:0055085 transmembrane transport | IEA GO_REF:0000002 | ACCEPT | Summary: Transmembrane transport. Reason: Correct, general. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Bidirectional proton-coupled monocarboxylate transporter |
| GO:0070062 extracellular exosome | HDA PMID:20458337 MHC class II-associated proteins in B-cell exosomes and pote... | KEEP AS NON CORE | Summary: Extracellular exosome (HDA, B-cell exosome proteomics). Reason: Plasma membrane proteins are routinely detected in exosome proteomes; not a site of MCT1 function. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates |
| GO:0071422 succinate transmembrane transport | IDA PMID:32946811 pH-Gated Succinate Secretion Regulates Muscle Remodeling in ... | KEEP AS NON CORE | Summary: Succinate transmembrane transport. Reason: Real, but a context-specific (acidified muscle) extension of the core monocarboxylate transport function. Supporting Evidence: PMID:32946811 succinate is rendered a transport substrate for monocarboxylate |
| GO:0097434 succinate:proton symporter activity | EXP PMID:32946811 pH-Gated Succinate Secretion Regulates Muscle Remodeling in ... | ACCEPT | Summary: Succinate:proton symporter activity (EXP). Reason: Supported by the Reddy et al. study showing MCT1-mediated pH-gated succinate export; Rhea:29303. Supporting Evidence: PMID:32946811 succinate is rendered a transport substrate for monocarboxylate file:human/SLC16A1/SLC16A1-uniprot.txt Facilitates the protonated monocarboxylate form of succinate export, |
| GO:0150104 transport across blood-brain barrier | NAS PMID:26590417 Establishment and Dysfunction of the Blood-Brain Barrier. | KEEP AS NON CORE | Summary: Transport across blood-brain barrier (NAS, reviews). Reason: MCT1 is expressed in brain endothelium and moves monocarboxylates (lactate, ketone bodies) across the BBB; a tissue context of the core transport activity. Cited reviews are general BBB reviews. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates |
| GO:0150104 transport across blood-brain barrier | NAS PMID:30280653 Blood-Brain Barrier: From Physiology to Disease and Back. | KEEP AS NON CORE | Summary: Transport across blood-brain barrier (NAS, reviews). Reason: MCT1 is expressed in brain endothelium and moves monocarboxylates (lactate, ketone bodies) across the BBB; a tissue context of the core transport activity. Cited reviews are general BBB reviews. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt rapid transport across the plasma membrane of many monocarboxylates |
| GO:1901475 pyruvate transmembrane transport | IEA GO_REF:0000107 | ACCEPT | Summary: Pyruvate transmembrane transport. Reason: MCT1 transports pyruvate (Rhea:64720). This is also the process that underlies exercise-induced hyperinsulinism when MCT1 is ectopically expressed in beta cells. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate PMID:17701893 induce SLC16A1 expression in beta cells, where this gene is not usually transcribed, permitting pyruvate uptake and pyruvate-stimulated insulin release despite ensuing hypoglycemia |
| GO:1902600 proton transmembrane transport | IEA GO_REF:0000108 | ACCEPT | Summary: Proton transmembrane transport (GO_REF:0000108, inferred from symporter activity). Reason: Protons are co-transported with each monocarboxylate; a direct consequence of the symport mechanism. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt Bidirectional proton-coupled monocarboxylate transporter |
| GO:1905039 carboxylic acid transmembrane transport | IEA GO_REF:0000107 | ACCEPT | Summary: Carboxylic acid transmembrane transport. Reason: Correct parent process. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate |
| GO:1905039 carboxylic acid transmembrane transport | ISS GO_REF:0000024 | ACCEPT | Summary: Carboxylic acid transmembrane transport. Reason: Correct parent process. Supporting Evidence: file:human/SLC16A1/SLC16A1-uniprot.txt such as lactate, pyruvate, acetate and the ketone bodies acetoacetate |
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Download this section (compressed HTML)Q: Which transcriptional or epigenetic mechanisms keep SLC16A1 silenced in pancreatic beta cells, and do the HHF7 promoter variants disrupt repressor binding or create activator sites?
Experiment: Map the beta-cell-specific silencing element of SLC16A1 (e.g. CRISPR tiling of the promoter in human stem-cell-derived beta cells) and test whether HHF7 variants abolish repression, linking ectopic MCT1 expression quantitatively to pyruvate-stimulated insulin secretion.
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