SMIM1 (small integral membrane protein 1) is a 78-amino-acid single-pass membrane protein of the erythrocyte plasma membrane that carries the Vel blood group antigen. It is expressed mainly in the erythroid lineage, with highest levels in bone marrow and induction during erythroid differentiation of CD34+ progenitors. The protein has a cytoplasmic N-terminal region bearing phosphoserines (Ser6, Ser17, Ser22, Ser27) and an acetylated initiator methionine, a single transmembrane helix near the C-terminus, and a very short extracellular C-terminal segment that displays the Vel epitope (type II / tail-anchored topology). SMIM1 forms disulfide-linked homodimers, through Cys77 and a transmembrane GxxxG motif, and dimerization promotes surface display of the Vel antigen. Homozygosity for a 17-bp frameshift deletion in exon 3 abolishes the protein and causes the Vel-negative phenotype, in which anti-Vel alloantibodies can cause haemolytic transfusion reactions. Its molecular function is unknown. Indirect evidence links it to red cell formation: an intronic eQTL that lowers SMIM1 expression is associated with lower mean corpuscular haemoglobin concentration, and smim1 morpholino knockdown in zebrafish mildly reduces red cell numbers. Humans lacking SMIM1 are healthy but show higher body weight, lower circulating thyroid hormone and reduced resting energy expenditure.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:A0A0S2Z4U3) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:O15393-2) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:O15552) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:O95377) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:P04233-2) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:P15151) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:P15941-11) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:P21964) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:P32942) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:P43628) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:P48165) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:P49447) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:P54219-3) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q13323) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q13520) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q15125) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q16623) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q3KNW5) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q4KMG9) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q5JX71) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q68DC2) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q6P9G4) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q6Y1H2) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q6ZSS7) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q7Z4F1) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q7Z5P4) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q86VR2) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q86WA9) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q86WV6) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q8N183) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q8N5K1) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q8N5M9) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q8N661) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q8NC24) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q8TAF8) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q8TBE3) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q8TBP5) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q8WWH4) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q96B21) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q96BA8) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q96DZ7) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q96KR6) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q96MV8) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q9BXK5) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q9GZY8-5) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q9H6H4) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q9HCJ2) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q9NR31) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q9NW97) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q9NY72) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q9Y282) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q9Y286) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q9Y320) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: High-throughput yeast two-hybrid hit (UniProtKB:Q9Y3Q0) from the HuRI human reference interactome; bare protein binding carries no functional information. Reason: Generic protein binding is uninformative and no more specific molecular function can be derived from a high-throughput Y2H pair. SMIM1 has 54 HuRI partners and none has any functional link to it. They are almost all unrelated membrane proteins (gap junction proteins, SLC transporters, syntaxin, ER/Golgi membrane proteins, mitochondrial outer-membrane proteins), which is the pattern expected when a single hydrophobic transmembrane helix is assayed in a yeast nuclear system. The only interaction with independent support is SMIM1 self-association, which is captured by the protein homodimerization activity row. Removal does not imply the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005886 plasma membrane | IBA GO_REF:0000033 | ACCEPT | Summary: Plasma membrane location propagated from the SMIM1 family node; SMIM1 is an established red cell plasma membrane protein. Reason: Consistent with direct experimental evidence on human SMIM1: it is purified from red cell membranes and displays the Vel antigen at the cell surface as a single-pass type II membrane protein. The IBA lists SMIM1 itself (UniProtKB:B2RUZ4) among its sources, which reflects this experimental grounding. Supporting Evidence: PMID:26452714 In conclusion, all our experimental data argue for SMIM1 being a type II membrane protein that displays the Vel antigen on its C-terminus. PMID:23563606 evolutionarily conserved transmembrane protein expressed on RBCs |
| GO:0005886 plasma membrane | IDA PMID:23563606 Homozygosity for a null allele of SMIM1 defines the Vel-nega... | ACCEPT | Summary: SMIM1 detected in red cell membranes; absent from Vel-negative RBC membranes. Reason: The abstract reports a transmembrane protein expressed on RBCs with a null phenotype in Vel-negative RBC membranes. The full text is not cached; the curated plasma membrane location is consistent with all other studies. Supporting Evidence: PMID:23563606 evolutionarily conserved transmembrane protein expressed on RBCs PMID:23563606 confirmed a null phenotype in RBC membranes, and SMIM1 overexpression induced |
| GO:0005886 plasma membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Plasma membrane from the UniProt subcellular location mapping (Cell membrane; single-pass type II membrane protein). Reason: The UniProt location is based on experimental topology and localization data, which support plasma membrane. Supporting Evidence: PMID:26452714 In conclusion, all our experimental data argue for SMIM1 being a type II membrane protein that displays the Vel antigen on its C-terminus. |
| GO:0005886 plasma membrane | IMP PMID:26452714 SMIM1 is a type II transmembrane phosphoprotein and displays... | ACCEPT | Summary: Topology and mutagenesis study in K-562 cells establishing SMIM1 as a type II plasma membrane protein displaying the Vel antigen. Reason: Direct evidence from flow cytometry of tagged and mutant SMIM1 in erythroleukaemia cells. Supporting Evidence: PMID:26452714 In conclusion, all our experimental data argue for SMIM1 being a type II membrane protein that displays the Vel antigen on its C-terminus. PMID:26452714 given that the expression of SMIM1 at the cell surface is essential for displaying the Vel antigen |
| GO:0009986 cell surface | IBA GO_REF:0000033 | ACCEPT | Summary: Cell surface location propagated from the SMIM1 family node; supported by flow cytometry of Vel antigen on red cells and transfected cells. Reason: The Vel epitope on the extracellular C-terminus of SMIM1 is detected on intact cells by flow cytometry, so SMIM1 is exposed at the cell surface. The target appears among its own IBA sources because it carries experimental annotations for this term. Supporting Evidence: PMID:23505126 Flow cytometry analysis of K-562 cells stably transfected with the SMIM1 cDNA showed cell-surface expression of the Vel antigen PMID:26452714 given that the expression of SMIM1 at the cell surface is essential for displaying the Vel antigen |
| GO:0009986 cell surface | IDA PMID:23505126 Disruption of SMIM1 causes the Vel- blood type. | ACCEPT | Summary: Flow cytometry shows Vel antigen on the surface of SMIM1-transfected K-562 cells and Vel+ red cells. Reason: Direct detection of the SMIM1-borne Vel epitope on intact cells. Supporting Evidence: PMID:23505126 Flow cytometry analysis of K-562 cells stably transfected with the SMIM1 cDNA showed cell-surface expression of the Vel antigen |
| GO:0009986 cell surface | IMP PMID:23563606 Homozygosity for a null allele of SMIM1 defines the Vel-nega... | ACCEPT | Summary: SMIM1 overexpression induces Vel antigen at the cell surface; loss of SMIM1 gives Vel-null red cells. Reason: Surface display of the Vel epitope depends on SMIM1 expression. Full text not cached, but the abstract supports the claim. Supporting Evidence: PMID:23563606 confirmed a null phenotype in RBC membranes, and SMIM1 overexpression induced |
| GO:0009986 cell surface | IMP PMID:26452714 SMIM1 is a type II transmembrane phosphoprotein and displays... | ACCEPT | Summary: Mutational analysis of SMIM1 surface expression (phosphosites, cysteines, C-terminal lysines) by flow cytometry. Reason: Cell-surface exposure of the SMIM1 C-terminus carrying the Vel antigen is shown directly. Supporting Evidence: PMID:26452714 given that the expression of SMIM1 at the cell surface is essential for displaying the Vel antigen PMID:26452714 In conclusion, all our experimental data argue for SMIM1 being a type II membrane protein that displays the Vel antigen on its C-terminus. |
| GO:0042803 protein homodimerization activity | IDA PMID:23563606 Homozygosity for a null allele of SMIM1 defines the Vel-nega... | KEEP AS NON CORE | Summary: SMIM1 forms disulfide-linked homodimers (about 32 kDa non-reduced versus 18 kDa reduced). Reason: Self-association is reproducibly shown: disulfide-dependent dimers in red cell membranes, Cys77 and a transmembrane GxxxG motif mediating dimerization, and spontaneous homodimers in a cell-free system. Dimerization promotes surface presentation of the Vel epitope. It is a structural property of the protein rather than its unknown physiological function, so it is kept as non-core. Supporting Evidence: PMID:23505126 we now know that SMIM1 can form molecular complexes (likely homodimers) via disulphide bonds PMID:31879955 dimerization is mediated both by an extracellular Cys77-dependent, homomeric PMID:32301496 is a tail-anchored transmembrane protein and readily forms homodimers in a cell-free system |
| GO:0048823 nucleate erythrocyte development | IBA GO_REF:0000033 | MODIFY | Summary: Nucleate erythrocyte development, propagated by IBA from zebrafish smim1 (ZFIN) morpholino knockdown data. Reason: The term is restricted to development of nucleated erythrocytes, the mature red cell type of fish, amphibians, reptiles and birds; human erythrocytes enucleate, so the term describes the wrong cell type for a human gene. The underlying signal is a mild reduction in red cell number after smim1 morpholino knockdown in zebrafish, supported in human by an intronic eQTL that lowers SMIM1 expression and is associated with lower mean corpuscular haemoglobin concentration. The taxon-neutral parent process, erythrocyte differentiation, captures this. The mechanism is unknown and the effect is small (Vel-negative humans have no recognised haematological disease), so this is a weakly supported, non-core process assignment; a full replacement term is proposed rather than removal because the IBA node placement within the vertebrate SMIM1 clade is not contradicted. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: LINEAGE OR TAXON MISMATCH Sources checked: PANTHER:PTN002866670 SUPPORTS SOURCE BUT NOT TARGET Vertebrate SMIM1 ancestral node; human SMIM1 is inside the clade, but the nucleate-erythrocyte term does not fit mammals, whose erythrocytes enucleate. ZFIN:ZDB-GENE-081022-87 SUPPORTS SOURCE BUT NOT TARGET Zebrafish smim1; experimental basis is morpholino knockdown with a mild reduction in red cell number, appropriate for a nucleated-erythrocyte species. Proposed replacements: erythrocyte differentiation Supporting Evidence: PMID:23563608 In vivo zebrafish smim1 knock down studies showed a mild reduction in the number of red blood cells, identifying SMIM1 as a novel regulator of red blood cell formation. |
Loading supporting content…
Download this section (compressed HTML)Q: Does SMIM1 have a molecular activity of its own (for example as a regulator of a red cell transporter or channel), or is it a structural component of an erythroid membrane complex?
Q: Is the zebrafish morpholino red cell phenotype reproduced in a stable smim1 mutant or a Smim1 knockout mouse, and is erythrocyte differentiation the right process term for human SMIM1?
Q: How does SMIM1 absence lower circulating thyroid hormone and energy expenditure, given its predominantly erythroid expression?
Experiment: Proximity labelling or co-immunoprecipitation of endogenous SMIM1 from primary erythroblasts to identify red cell membrane partners, replacing the non-specific Y2H interactome.
Experiment: Generate Smim1 knockout mice (or stable zebrafish mutants) and measure erythropoiesis, red cell indices, thyroid hormone levels and energy expenditure.
Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)