SMIM10L1 is a 68-amino-acid protein of the SMIM10 family (Pfam DUF4560), paralogous to SMIM10, with a disordered alanine/proline-rich N-terminus and a hydrophobic C-terminal half predicted to be alpha-helical and membrane-associated. It is expressed in all tissues. A high-confidence proteomic inventory of human mitochondria includes it. In adipose progenitor cells its transcript falls sharply after PTEN knockdown, and its own knockdown increases PI3K/AKT/mTOR signalling and enhances adipocyte differentiation. Its membrane topology, mitochondrial sub-compartment and molecular activity are unknown.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005739 mitochondrion | HTP PMID:34800366 Quantitative high-confidence human mitochondrial proteome an... | ACCEPT | Summary: High-throughput assignment to mitochondria from the MitoCoP high-confidence human mitochondrial proteome. Reason: MitoCoP assigns proteins by combining subtractive and spatial proteomics, import assays and curation under stringent criteria. The per-protein evidence for SMIM10L1 sits in supplementary tables not in our cache, so we defer to the curator. It is the only location evidence for SMIM10L1 and fits a small protein with a hydrophobic C-terminal segment. The mitochondrial sub-compartment is not established. Supporting Evidence: PMID:34800366 We defined a human mitochondrial high-confidence proteome (MitoCoP) of 1,134 protein-coding genes, including 91 proteins previously not localized to mitochondria experimentally |
Loading supporting content…
Download this section (compressed HTML)Q: Is SMIM10L1 an integral mitochondrial membrane protein, and in which membrane (outer or inner) and orientation does it sit?
Q: How does loss of a mitochondrial microprotein lower PTEN and increase PI3K/AKT/mTOR signalling in adipose progenitors: directly or through a metabolic signal?
Experiment: Fractionate mitochondria from cells expressing endogenously tagged SMIM10L1, test carbonate resistance and protease protection with outer- and inner-membrane markers, and confirm by immunofluorescence or proximity labelling.
Hypothesis: SMIM10L1 is a mitochondrial membrane protein.
Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)