SMIM15 (formerly C5orf43) is a 74-amino-acid (about 8.6 kDa) protein of the SMIM15 family (Pfam UPF0542). It has a single predicted transmembrane helix (residues 20-40) followed by a predicted coiled-coil region and a short, disordered C-terminal tail rich in lysine and arginine. The transcript is detected in all tissues, with highest single-cell levels in oesophageal epithelium. The membrane in which SMIM15 resides, its orientation, and any binding partner, molecular activity or biological process are unknown; the gene appears in the literature only within multi-gene expression and genomic signatures.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0016020 membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Electronic mapping of the UniProt subcellular location "Membrane; Single-pass membrane protein", which is itself inferred from the predicted transmembrane helix. Reason: Correct but uninformative. A 21-residue hydrophobic helix is a reliable indicator of membrane integration. No experimental evidence identifies the organelle, so this generic term is the most specific one that can be supported. Supporting Evidence: UniProt:Q7Z3B0 SUBCELLULAR LOCATION: Membrane |
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Download this section (compressed HTML)Q: Where does endogenous SMIM15 reside, and does its basic C-terminal tail face the cytosol as the positive-inside rule would predict?
Experiment: Determine the localisation of endogenously tagged SMIM15 in an oesophageal epithelial cell line by co-staining with organelle markers, confirm membrane integration by carbonate extraction, and determine topology by protease protection.
Hypothesis: SMIM15 is an integral membrane protein of a defined organelle.
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