SMIM3 (small integral membrane protein 3; also NID67, NGF-induced differentiation clone 67) encodes a 60-residue protein with a single predicted central transmembrane helix and little else; it is conserved in mammals (rat and human about 85% identical). The gene was identified in rat PC12 cells as a primary-response gene induced by NGF and FGF, and also by forskolin, calcium ionophore and ATP; its mRNA is most abundant in heart, ovary and adrenal gland. In vitro translation confirms that the open reading frame yields a ~6 kDa product. No molecular activity, binding partner of functional consequence, or biological process has been established. Its similarity to other small single-helix membrane proteins that modulate ion channels led to the suggestion of a channel-regulatory role, which remains untested. In acute myeloid leukemia, SMIM3 mRNA is elevated and associated with poorer survival, and shRNA knockdown slowed growth of AML cell lines, but this has not been linked to a molecular mechanism. The mouse ortholog marks one end of the Cd74-Nid67 interval used to model the 5q- myelodysplastic syndrome, whose phenotype is attributed to Rps14 haploinsufficiency rather than to Nid67.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005515 protein binding | IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in... | REMOVE | Summary: Binary interaction with BSCL2 (UniProtKB:Q96G97) from the CCSB-HI1 interactome screen (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:16189514 Using a stringent, high-throughput yeast two-hybrid system, we tested pairwise interactions among the products of approximately 8,100 currently available Gateway-cloned open reading frames |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: Binary interaction with MAL (UniProtKB:P21145) from the HI-II-14 human interactome map (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:25416956 Here, we describe a systematic map of ?14,000 high-quality human binary protein-protein interactions. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: Binary interaction with ATP6V0C (UniProtKB:P27449) from the HI-II-14 human interactome map (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:25416956 Here, we describe a systematic map of ?14,000 high-quality human binary protein-protein interactions. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: Binary interaction with EMP1 (UniProtKB:P54849) from the HI-II-14 human interactome map (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:25416956 Here, we describe a systematic map of ?14,000 high-quality human binary protein-protein interactions. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: Binary interaction with PMP22 (UniProtKB:Q01453) from the HI-II-14 human interactome map (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:25416956 Here, we describe a systematic map of ?14,000 high-quality human binary protein-protein interactions. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: Binary interaction with PNMA1 (UniProtKB:Q8ND90) from the HI-II-14 human interactome map (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:25416956 Here, we describe a systematic map of ?14,000 high-quality human binary protein-protein interactions. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: Binary interaction with BSCL2 (UniProtKB:Q96G97) from the HI-II-14 human interactome map (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:25416956 Here, we describe a systematic map of ?14,000 high-quality human binary protein-protein interactions. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: Binary interaction with BSCL2 (UniProtKB:Q96G97-4) from the HI-II-14 human interactome map (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:25416956 Here, we describe a systematic map of ?14,000 high-quality human binary protein-protein interactions. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: Binary interaction with LZTS2 (UniProtKB:Q9BRK4) from the HI-II-14 human interactome map (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:25416956 Here, we describe a systematic map of ?14,000 high-quality human binary protein-protein interactions. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | REMOVE | Summary: Binary interaction with CLEC7A (UniProtKB:Q9BXN2) from the HI-II-14 human interactome map (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:25416956 Here, we describe a systematic map of ?14,000 high-quality human binary protein-protein interactions. |
| GO:0005515 protein binding | IPI PMID:25910212 Widespread macromolecular interaction perturbations in human... | REMOVE | Summary: Binary interaction with SFTPC (UniProtKB:P11686) from a screen of wild-type and disease-mutant interaction profiles (Sahni et al. 2015); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:25910212 Here we functionally profile several thousand missense mutations across a spectrum of Mendelian disorders using various interaction assays. |
| GO:0005515 protein binding | IPI PMID:25910212 Widespread macromolecular interaction perturbations in human... | REMOVE | Summary: Binary interaction with PMP22 (UniProtKB:Q01453) from a screen of wild-type and disease-mutant interaction profiles (Sahni et al. 2015); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:25910212 Here we functionally profile several thousand missense mutations across a spectrum of Mendelian disorders using various interaction assays. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with TMEM218 (UniProtKB:A2RU14) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with BSCL2 (UniProtKB:J3KQ12) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with CLDN5 (UniProtKB:O00501) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with SLC22A1 (UniProtKB:O15245) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with GPR42 (UniProtKB:O15529) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with CLDN11 (UniProtKB:O75508) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with ZFPL1 (UniProtKB:O95159) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with CNIH1 (UniProtKB:O95406) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with ASGR2 (UniProtKB:P07307-3) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with CD79A (UniProtKB:P11912) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with CD53 (UniProtKB:P19397) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with MAL (UniProtKB:P21145) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with ATP6V0C (UniProtKB:P27449) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with CD82 (UniProtKB:P27701) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with IFNGR2 (UniProtKB:P38484) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with LPAR6 (UniProtKB:P43657) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with EMP1 (UniProtKB:P54849) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with EMP3 (UniProtKB:P54852) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with CLDN8 (UniProtKB:P56748) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with PLP1 (UniProtKB:P60201-2) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with PMP22 (UniProtKB:Q01453) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with SGCZ (UniProtKB:Q08AT0) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with BNIP3 (UniProtKB:Q12983) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with EBP (UniProtKB:Q15125) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with SGCB (UniProtKB:Q16585) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with STX1A (UniProtKB:Q16623) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with BTNL9 (UniProtKB:Q6UXG8-3) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with TMEM182 (UniProtKB:Q6ZP80) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with CLEC17A (UniProtKB:Q6ZS10) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with LSMEM2 (UniProtKB:Q8N112) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with KIR3DL3 (UniProtKB:Q8N743) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with CNIH3 (UniProtKB:Q8TBE1) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with FNDC9 (UniProtKB:Q8TBE3) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with TM4SF18 (UniProtKB:Q96CE8) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with GIMAP5 (UniProtKB:Q96F15) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with MS4A3 (UniProtKB:Q96HJ5) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with SEC22A (UniProtKB:Q96IW7) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with PDCD1LG2 (UniProtKB:Q9BQ51) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with CLEC7A (UniProtKB:Q9BXN2-6) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with SLC38A1 (UniProtKB:Q9H2H9) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with SPACA1 (UniProtKB:Q9HBV2) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with SMCO4 (UniProtKB:Q9NRQ5) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with RPRM (UniProtKB:Q9NS64) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with ATP1B4 (UniProtKB:Q9UN42) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with ERGIC3 (UniProtKB:Q9Y282) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | REMOVE | Summary: Binary interaction with TEX264 (UniProtKB:Q9Y6I9) from the HuRI human reference interactome (yeast two-hybrid); bare protein binding carries no functional information. Reason: Generic protein binding is uninformative, and no more specific molecular function can be derived from a high-throughput binary-interaction hit, so the row is removed rather than rewritten. Across the four interactome screens SMIM3 has about 55 partners, almost all of them unrelated membrane proteins (PMP22, MAL, BSCL2, CNIH1/3, EMP1/3, claudins, tetraspanins, ATP6V0C, SLC transporters, C-type lectins). This broad pattern is what a single hydrophobic transmembrane helix typically produces in yeast two-hybrid assays, and none of the pairs has been followed up with a functional readout. Removal does not imply that the reported interaction is false. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
| GO:0016020 membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Membrane location mapped from the UniProt subcellular-location annotation (single-pass membrane protein, by similarity/sequence). Reason: SMIM3 has one predicted transmembrane helix (residues 20-40) and essentially no other domain; the original cloning paper also inferred a transmembrane domain. Which membrane it resides in has not been determined, so the generic term is the appropriate level. Supporting Evidence: PMID:11288140 The central 29 amino acids are very hydrophobic and very likely comprise a transmembrane domain. UniProt:Q9BZL3 SUBCELLULAR LOCATION: Membrane |
| GO:0042802 identical protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: SMIM3 self-interaction detected once in the HuRI yeast two-hybrid screen. Reason: A single high-throughput Y2H self-pair for a 60-residue peptide whose only feature is a transmembrane helix. Homo-oligomerisation of single-pass helices is common and is not contradicted, but it has not been tested by an orthogonal method in membranes, and no functional consequence is known, so the term does not describe a function of SMIM3. Supporting Evidence: PMID:32296183 Specifically, yeast two-hybrid (Y2H) represents the only binary PPI assay that can be operated at sufficient throughput to systematically screen the human proteome for binary PPIs. |
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Download this section (compressed HTML)Q: Which membrane does endogenous SMIM3 occupy (plasma membrane, ER, Golgi), and what is its topology? No localization experiment with endogenous or tagged protein has been reported.
Q: Does SMIM3 modulate any ion channel or pump, as proposed by analogy to other single-helix membrane regulators (e.g. FXYD proteins, regulins)? Among the ~55 interactome partners, are any (e.g. ATP1B4, ATP6V0C, PMP22) reproducible in membranes by co-immunoprecipitation or proximity labelling?
Q: Is the reduced proliferation of AML cells after SMIM3 knockdown reproducible with independent guides or CRISPR knockout and rescued by an shRNA-resistant cDNA?
Experiment: Tag endogenous SMIM3 in a neuronal or adrenal cell line, determine its compartment by imaging, identify partners by proximity labelling (e.g. TurboID) with a membrane-protein control, and test candidate channel or pump activity electrophysiologically or by flux assays with and without SMIM3.
Hypothesis: SMIM3 is an NGF-inducible single-pass membrane protein that associates with and regulates a specific membrane transporter or channel.
Type: proximity labelling and functional assay
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