SMIM41 (small integral membrane protein 41) is a 93-amino-acid (about 9 kDa) protein encoded on chromosome 12q13.13. It has a single predicted helical transmembrane segment (residues 38-58), preceded by an alanine/serine/proline-rich N-terminal segment and followed by a predicted disordered, acidic C-terminal tail. It is a member of the Reprimo family of small single-pass membrane proteins, alongside RPRM (Reprimo) and RPRML, but forms its own subfamily; the cell-cycle role proposed for Reprimo has not been tested for SMIM41 and cannot be inferred from family membership. Orthologues are annotated in many placental mammals, including mouse and rat. The protein has been detected by mass spectrometry, and its RNA is enhanced in lung and prostate. Its membrane localisation is predicted from sequence; the specific membrane, topology, partners and biological role are unknown, and no functional study of SMIM41 has been published.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0016020 membrane | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt subcellular-location mapping to membrane, based on the single predicted helical transmembrane segment (residues 38-58). Reason: Correct, though general. A 21-residue hydrophobic helix in a 93-residue protein makes SMIM41 a single-pass integral membrane protein, like the other Reprimo family members. No experiment identifies the specific membrane, so a more precise location cannot be given. This is the only annotation the gene carries and the only one the evidence supports. Supporting Evidence: file:human/SMIM41/SMIM41-uniprot.txt FT TRANSMEM 38..58 file:human/SMIM41/SMIM41-uniprot.txt CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane |
Loading supporting content…
Download this section (compressed HTML)Q: Does SMIM41 share any activity with Reprimo (RPRM), for example a role downstream of p53 or in cell-cycle arrest, or did it diverge after the subfamily split?
Q: Which lung and prostate cell types express SMIM41 protein, and in which membrane?
Experiment: Localise endogenously tagged SMIM41 in a lung epithelial cell line with organelle markers, test N-glycosylation of the Asn2 sequon to establish topology, and test whether its expression responds to p53 activation as Reprimo's does.
Hypothesis: SMIM41 is a single-pass membrane protein of a defined compartment in lung or prostate epithelium.
Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)