TRAPPC1 is a small core subunit of the transport protein particle (TRAPP) complex. It contributes to TRAPP complex architecture and complex-level RAB1 guanine-nucleotide exchange/vesicle-trafficking functions, especially ER-to-Golgi vesicle-mediated transport. Autophagy component recruitment is best understood through TRAPP/TRAPPII/TRAPPIII complex membership rather than independent TRAPPC1 activity.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0030008 TRAPP complex | IBA GO_REF:0000033 | ACCEPT | Summary: TRAPPC1 is a core subunit of the TRAPP complex, and this cellular-component annotation captures the strongest gene-level function. Reason: Accept as core. Direct structural and review evidence place TRAPPC1/Mum2 in the conserved TRAPP core, and the PN mapping also resolves the autophagy-contextual leaf to TRAPP complex membership. Supporting Evidence: PMID:16828797 crystal structure of a human Bet3-Tpc6B heterodimer, which represents a core sub-complex in the assembly of TRAPP PMID:16828797 Bet3-Tpc6A and Bet3-Tpc6B, are able to recruit Mum2, a further TRAPP subunit PMID:27066478 TRAPP is a highly conserved modular multi-subunit protein complex PMID:27066478 Core TRAPP contains four small subunits that self assemble to a stable complex, which has a GEF activity on Ypt1 |
| GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport | IBA GO_REF:0000033 | ACCEPT | Summary: TRAPPC1 contributes to TRAPP-mediated ER-to-Golgi vesicle-mediated transport. Reason: Accept as a core process. TRAPPC complexes activate RAB1 and facilitate ER-to-Golgi traffic, and TRAPPC1 is a core TRAPP subunit. Supporting Evidence: PMID:27066478 Bet3, which mediates endoplasmic reticulum (ER)-to-Golgi transport Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:0005737 cytoplasm | IEA GO_REF:0000117 | ACCEPT | Summary: TRAPPC1 is a cytoplasmic/cytosolic TRAPP subunit associated with membrane-trafficking complexes. Reason: Accept as a broad supported cellular location for a peripheral trafficking-complex subunit. Supporting Evidence: PMID:27066478 TRAPP is a highly conserved modular multi-subunit protein complex Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it |
| GO:0005783 endoplasmic reticulum | IEA GO_REF:0000044 | ACCEPT | Summary: TRAPPC1 participates in ER-derived vesicle trafficking and UniProt maps it to the endoplasmic reticulum. Reason: Accept as a supported location for the ER-to-Golgi trafficking context. Supporting Evidence: PMID:27066478 Bet3, which mediates endoplasmic reticulum (ER)-to-Golgi transport Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694439 TRAPPC is a guanine-nucleotide exchange factor for RAB1 and is recruited to ER-derived vesicles by virtue of an interaction between the TRAPPC component TRAPPC3 and the coat protein SEC23 |
| GO:0005794 Golgi apparatus | IEA GO_REF:0000044 | ACCEPT | Summary: TRAPPC1 participates in ER-to-Golgi transport and is associated with Golgi/cis-Golgi TRAPP trafficking functions. Reason: Accept as a supported Golgi-location annotation for TRAPP-mediated ER-to-Golgi trafficking. Falcon deep research corroborates Golgi/ER-Golgi-interface localization for TRAPPC1 as part of TRAPP complexes (with cis-Golgi labeling reported for TRAPP-specific subunits in Drosophila). Supporting Evidence: PMID:27066478 Bet3, which mediates endoplasmic reticulum (ER)-to-Golgi transport Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic file:human/TRAPPC1/TRAPPC1-deep-research-falcon.md TRAPPC1, as part of TRAPP complexes, localizes primarily to the Golgi apparatus and the endoplasmic reticulum (ER)-Golgi interface |
| GO:0016192 vesicle-mediated transport | IEA GO_REF:0000002 | MODIFY | Summary: Vesicle-mediated transport is true but too broad for TRAPPC1; the evidence supports the ER-to-Golgi/TRAPP/RAB1 pathway more specifically. Reason: Modify to the more specific ER-to-Golgi vesicle-mediated transport term. Proposed replacements: endoplasmic reticulum to Golgi vesicle-mediated transport Supporting Evidence: PMID:27066478 Bet3, which mediates endoplasmic reticulum (ER)-to-Golgi transport Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it |
| GO:0030008 TRAPP complex | IEA GO_REF:0000120 | ACCEPT | Summary: TRAPPC1 is a core subunit of the TRAPP complex, and this cellular-component annotation captures the strongest gene-level function. Reason: Accept as core. Direct structural and review evidence place TRAPPC1/Mum2 in the conserved TRAPP core, and the PN mapping also resolves the autophagy-contextual leaf to TRAPP complex membership. Supporting Evidence: PMID:16828797 crystal structure of a human Bet3-Tpc6B heterodimer, which represents a core sub-complex in the assembly of TRAPP PMID:16828797 Bet3-Tpc6A and Bet3-Tpc6B, are able to recruit Mum2, a further TRAPP subunit PMID:27066478 TRAPP is a highly conserved modular multi-subunit protein complex PMID:27066478 Core TRAPP contains four small subunits that self assemble to a stable complex, which has a GEF activity on Ypt1 |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC1. Reason: Mark as over-annotated. Specific interaction biology should be represented by TRAPP/TRAPPII/TRAPPIII complex membership rather than GO:0005515. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC1. Reason: Mark as over-annotated. Specific interaction biology should be represented by TRAPP/TRAPPII/TRAPPIII complex membership rather than GO:0005515. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC1. Reason: Mark as over-annotated. Specific interaction biology should be represented by TRAPP/TRAPPII/TRAPPIII complex membership rather than GO:0005515. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC1. Reason: Mark as over-annotated. Specific interaction biology should be represented by TRAPP/TRAPPII/TRAPPIII complex membership rather than GO:0005515. |
| GO:0005737 cytoplasm | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | ACCEPT | Summary: TRAPPC1 is a cytoplasmic/cytosolic TRAPP subunit associated with membrane-trafficking complexes. Reason: Accept as a broad supported cellular location for a peripheral trafficking-complex subunit. Supporting Evidence: PMID:27066478 TRAPP is a highly conserved modular multi-subunit protein complex Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it |
| GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | ACCEPT | Summary: TRAPPC1 contributes to TRAPP-mediated ER-to-Golgi vesicle-mediated transport. Reason: Accept as a core process. TRAPPC complexes activate RAB1 and facilitate ER-to-Golgi traffic, and TRAPPC1 is a core TRAPP subunit. Supporting Evidence: PMID:27066478 Bet3, which mediates endoplasmic reticulum (ER)-to-Golgi transport Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III file:human/TRAPPC1/TRAPPC1-deep-research-falcon.md TRAPPIII activates Rab1 to regulate transport of newly synthesized proteins from the ER to the Golgi apparatus |
| GO:0006901 vesicle coat assembly | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | MODIFY | Summary: TRAPPC complexes interact with vesicle coats and RAB1 on ER-derived vesicles, but TRAPPC1 is not a coat-assembly factor. Reason: Modify to ER-to-Golgi vesicle-mediated transport. The coat-related evidence supports recruitment/traffic context rather than direct vesicle coat assembly. Proposed replacements: endoplasmic reticulum to Golgi vesicle-mediated transport Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a guanine-nucleotide exchange factor for RAB1 and is recruited to ER-derived vesicles by virtue of an interaction between the TRAPPC component TRAPPC3 and the coat protein SEC23 Reactome:R-HSA-8877475 TRAPPCII is recruited to ER-derived vesicles by virtue of an interaction between the TRAPPCII component TRAPPC3 and the COPII coat protein SEC23 |
| GO:0048208 COPII vesicle coat assembly | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | MODIFY | Summary: The COPII evidence places TRAPPCII near SEC23/COPII vesicles, but does not show TRAPPC1 assembling the COPII coat. Reason: Modify to ER-to-Golgi vesicle-mediated transport, which captures the supported TRAPP/COPII/RAB1 trafficking role without overclaiming coat assembly. Proposed replacements: endoplasmic reticulum to Golgi vesicle-mediated transport Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a guanine-nucleotide exchange factor for RAB1 and is recruited to ER-derived vesicles by virtue of an interaction between the TRAPPC component TRAPPC3 and the coat protein SEC23 Reactome:R-HSA-8877475 TRAPPCII is recruited to ER-derived vesicles by virtue of an interaction between the TRAPPCII component TRAPPC3 and the COPII coat protein SEC23 |
| GO:0099022 obsolete vesicle tethering | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | MODIFY | Summary: This annotation uses an obsolete vesicle-tethering term, and the cited TRAPP review cautions that direct TRAPP tethering evidence is inconclusive. Reason: Modify to ER-to-Golgi vesicle-mediated transport, the safer supported process for TRAPPC1/TRAPP complex function. Proposed replacements: endoplasmic reticulum to Golgi vesicle-mediated transport Supporting Evidence: PMID:27066478 evidence that any TRAPP complex acts as a membrane tether is currently inconclusive Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic |
| GO:1990071 TRAPPII protein complex | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | ACCEPT | Summary: TRAPPC1 is represented in human TRAPPII complex models and ComplexPortal annotations. Reason: Accept as a supported TRAPP complex subtype membership, while recognizing that gene-level process conclusions should remain centered on ER-Golgi transport and TRAPP/RAB1 function. Supporting Evidence: PMID:27066478 TRAPP is a highly conserved modular multi-subunit protein complex PMID:27066478 Core TRAPP contains four small subunits that self assemble to a stable complex, which has a GEF activity on Ypt1 Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III Reactome:R-HSA-8877475 Interaction of TRAAPPCII with RAB1:GDP promotes release of GDP, allowing GTP to bind |
| GO:1990072 TRAPPIII protein complex | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | ACCEPT | Summary: TRAPPC1 is represented in human TRAPPIII complex models; this is the TRAPP subtype most relevant to the PN autophagy recruitment context. Reason: Accept as supported complex membership. The autophagy implication should be treated as context for TRAPPIII/RAB1 biology, not as a broad autophagy BP annotation for TRAPPC1. Falcon deep research likewise links autophagosome formation to the TRAPPIII holo-complex rather than to TRAPPC1 specifically. Supporting Evidence: PMID:27066478 TRAPP is a highly conserved modular multi-subunit protein complex PMID:27066478 Core TRAPP contains four small subunits that self assemble to a stable complex, which has a GEF activity on Ypt1 Reactome:R-HSA-8877475 RAB1 and the TRAPPCIII complex play a role in the formation of the pre-autophagosomal structure (PAS) and contribute to the localization of ATG9 PMID:27066478 the connection of the mammalian TRAPP III complex to autophagy is currently not clear file:human/TRAPPC1/TRAPPC1-deep-research-falcon.md TRAPPIII has been localized to autophagy-related membranes where it participates in autophagosome formation |
| GO:0005576 extracellular region | TAS Reactome:R-HSA-6798751 | MARK AS OVER ANNOTATED | Summary: The extracellular-region annotation comes from a broad Reactome neutrophil-degranulation event and is not supported as a core TRAPPC1 localization. Reason: Mark as over-annotated. TRAPPC1 is a TRAPP trafficking-complex subunit associated with cytosol/ER/Golgi contexts, not a secreted extracellular protein. Supporting Evidence: Reactome:R-HSA-6798751 Azurophil or primary granules were originally defined by their high content of myeloperoxidase |
| GO:0035578 azurophil granule lumen | TAS Reactome:R-HSA-6798751 | MARK AS OVER ANNOTATED | Summary: The azurophil-granule-lumen annotation is inherited from a broad Reactome degranulation context and lacks TRAPPC1-specific support. Reason: Mark as over-annotated. It does not fit the core TRAPPC1/TRAPP complex localization or trafficking function. Supporting Evidence: Reactome:R-HSA-6798751 Azurophil or primary granules were originally defined by their high content of myeloperoxidase |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5694409 | ACCEPT | Summary: Reactome places TRAPPC1-containing TRAPPC events in the cytosol/cytosolic face of ER-Golgi trafficking reactions. Reason: Accept as a supported location for TRAPPC/RAB1 trafficking reactions. Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III Reactome:R-HSA-8877475 Interaction of TRAAPPCII with RAB1:GDP promotes release of GDP, allowing GTP to bind |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5694418 | ACCEPT | Summary: Reactome places TRAPPC1-containing TRAPPC events in the cytosol/cytosolic face of ER-Golgi trafficking reactions. Reason: Accept as a supported location for TRAPPC/RAB1 trafficking reactions. Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III Reactome:R-HSA-8877475 Interaction of TRAAPPCII with RAB1:GDP promotes release of GDP, allowing GTP to bind |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5694439 | ACCEPT | Summary: Reactome places TRAPPC1-containing TRAPPC events in the cytosol/cytosolic face of ER-Golgi trafficking reactions. Reason: Accept as a supported location for TRAPPC/RAB1 trafficking reactions. Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III Reactome:R-HSA-8877475 Interaction of TRAAPPCII with RAB1:GDP promotes release of GDP, allowing GTP to bind |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5694441 | ACCEPT | Summary: Reactome places TRAPPC1-containing TRAPPC events in the cytosol/cytosolic face of ER-Golgi trafficking reactions. Reason: Accept as a supported location for TRAPPC/RAB1 trafficking reactions. Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III Reactome:R-HSA-8877475 Interaction of TRAAPPCII with RAB1:GDP promotes release of GDP, allowing GTP to bind |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8877475 | ACCEPT | Summary: Reactome places TRAPPC1-containing TRAPPC events in the cytosol/cytosolic face of ER-Golgi trafficking reactions. Reason: Accept as a supported location for TRAPPC/RAB1 trafficking reactions. Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III Reactome:R-HSA-8877475 Interaction of TRAAPPCII with RAB1:GDP promotes release of GDP, allowing GTP to bind |
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Download this section (compressed HTML)Q: Should TRAPPC1 and other shared TRAPP subunits receive contributes_to annotations for TRAPPC complex RAB1 guanine-nucleotide exchange activity?
Suggested experts: GO transport editors, Reactome TRAPP curators
Q: Should TRAPP subunit PN autophagy contexts be captured only as TRAPPIII complex membership unless direct human autophagophore/PAS evidence is available?
Suggested experts: GO autophagy editors, GO cellular-component editors
Q: Should current TRAPPC1 vesicle coat assembly and obsolete vesicle tethering annotations be replaced by ER-to-Golgi vesicle-mediated transport or more specific TRAPP/RAB1 terms?
Suggested experts: ComplexPortal curators, GO transport editors
Experiment: Reconstitute human TRAPPII/TRAPPIII complexes with and without TRAPPC1 and measure RAB1 GDP-GTP exchange activity.
Hypothesis: TRAPPC1 is required as a core scaffold subunit for TRAPPC complex-level RAB1 GEF activity rather than acting as an independent catalytic subunit.
Type: complex reconstitution and RAB1 GEF assay
Experiment: Use TRAPPC1 knockout and rescue cells to assay ER-to-Golgi cargo transport, COPII-vesicle association, and Golgi localization of TRAPP components.
Hypothesis: TRAPPC1 loss disrupts TRAPP-dependent ER-to-Golgi vesicle-mediated transport by destabilizing or mislocalizing TRAPP complexes.
Type: cellular trafficking rescue assay
Experiment: Measure ATG9 localization and autophagophore initiation markers in TRAPPC1 rescue cells while separately monitoring ER-to-Golgi trafficking defects.
Hypothesis: Any TRAPPC1 autophagy phenotype is mediated through TRAPPIII/RAB1 trafficking context and should not be generalized to a broad autophagy core function without direct evidence.
Type: autophagy-context separation assay
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