TRAPPC3 is the human Bet3 homolog and a core TRAPP-complex subunit. It contributes to complex-level RAB1 guanine-nucleotide exchange factor activity, TRAPP/TRAPPII/TRAPPIII complex architecture, and ER-to-Golgi vesicle-mediated transport. Autophagophore-recruitment effects are best understood through TRAPP complex membership and TRAPPIII/RAB1 context rather than independent TRAPPC3-specific autophagy activity.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005085 guanyl-nucleotide exchange factor activity | IBA GO_REF:0000033 | ACCEPT | Summary: TRAPPC3 contributes to complex-level RAB1 guanine-nucleotide exchange factor activity as a TRAPP subunit. Reason: Accept with the contributes_to qualifier. The TRAPPC complex, not isolated TRAPPC3, provides the GEF activity for RAB1/Ypt1-family GTPases. Supporting Evidence: Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III PMID:21525244 the complex acts as a guanine nucleotide exchange factor (GEF) for Ypt1p |
| GO:0005829 cytosol | IBA GO_REF:0000033 | ACCEPT | Summary: TRAPPC3-containing TRAPP reactions occur on the cytosolic/cytoplasmic side of ER-Golgi trafficking membranes. Reason: Accept as supported location for a peripheral trafficking-complex subunit. Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport | IBA GO_REF:0000033 | ACCEPT | Summary: TRAPPC3-containing TRAPP complexes mediate ER-to-Golgi vesicle traffic through TRAPP/RAB1 biology. Reason: Accept as a core process for TRAPPC3. Multiple human/mammalian TRAPP sources and Reactome support ER-to-Golgi trafficking. Supporting Evidence: PMID:18930054 The transport protein particle (TRAPP) complex is required for proper vesicular transport from the ER to the Golgi PMID:21525244 C4orf41 and TTC-15 (which we call TRAPPC11 and TRAPPC12, respectively) in ER-to-Golgi trafficking at a very early stage Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it |
| GO:0006891 intra-Golgi vesicle-mediated transport | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Intra-Golgi transport is plausible through TRAPPII/Golgi trafficking context but is less central than ER-to-Golgi transport for human TRAPPC3. Reason: Keep as non-core. The TRAPPII/Golgi evidence supports a context-specific trafficking role, while the core TRAPPC3 process remains ER-to-Golgi/TRAPP/RAB1 transport. Supporting Evidence: PMID:21453443 for distinct tethering events at Golgi membranes PMID:18930054 different functions in the regulation of ER-to-Golgi traffic |
| GO:0030008 TRAPP complex | IBA GO_REF:0000033 | ACCEPT | Summary: TRAPPC3/Bet3 is a core TRAPP complex subunit. Reason: Accept as core cellular-component membership. This is the cleanest gene-level representation of the PN TRAPP component leaf. Supporting Evidence: PMID:16828797 crystal structure of a human Bet3-Tpc6B heterodimer, which represents a core sub-complex in the assembly of TRAPP PMID:16828797 Bet3-Tpc6A and Bet3-Tpc6B, are able to recruit Mum2, a further TRAPP subunit PMID:21525244 the mammalian orthologues named TRAPPC1, TRAPPC2, TRAPPC3, TRAPPC4, TRAPPC5, and TRAPPC6a/b |
| GO:0033106 cis-Golgi network membrane | IBA GO_REF:0000033 | ACCEPT | Summary: cis-Golgi network membrane is a supported active site for TRAPPC3-containing TRAPP complexes. Reason: Accept as a supported active location for TRAPP/RAB1 ER-to-Golgi trafficking. Supporting Evidence: PMID:18930054 The transport protein particle (TRAPP) complex is required for proper vesicular transport from the ER to the Golgi Reactome:R-HSA-5694439 TRAPPC is a guanine-nucleotide exchange factor for RAB1 and is recruited to ER-derived vesicles by virtue of an interaction between the TRAPPC component TRAPPC3 and the coat protein SEC23 |
| GO:0005783 endoplasmic reticulum | IEA GO_REF:0000044 | ACCEPT | Summary: TRAPPC3 participates in ER-derived vesicle trafficking and is mapped to the endoplasmic reticulum. Reason: Accept as a supported location for ER-to-Golgi trafficking. Supporting Evidence: PMID:18930054 The transport protein particle (TRAPP) complex is required for proper vesicular transport from the ER to the Golgi Reactome:R-HSA-5694439 TRAPPC is a guanine-nucleotide exchange factor for RAB1 and is recruited to ER-derived vesicles by virtue of an interaction between the TRAPPC component TRAPPC3 and the coat protein SEC23 |
| GO:0005794 Golgi apparatus | IEA GO_REF:0000044 | ACCEPT | Summary: TRAPPC3 functions in TRAPP trafficking at Golgi/cis-Golgi membranes. Reason: Accept as supported location for TRAPP-mediated ER-to-Golgi and Golgi trafficking. Supporting Evidence: PMID:18930054 The transport protein particle (TRAPP) complex is required for proper vesicular transport from the ER to the Golgi PMID:21453443 for distinct tethering events at Golgi membranes PMID:18930054 different functions in the regulation of ER-to-Golgi traffic |
| GO:0030008 TRAPP complex | IEA GO_REF:0000120 | ACCEPT | Summary: TRAPPC3/Bet3 is a core TRAPP complex subunit. Reason: Accept as core cellular-component membership. This is the cleanest gene-level representation of the PN TRAPP component leaf. Supporting Evidence: PMID:16828797 crystal structure of a human Bet3-Tpc6B heterodimer, which represents a core sub-complex in the assembly of TRAPP PMID:16828797 Bet3-Tpc6A and Bet3-Tpc6B, are able to recruit Mum2, a further TRAPP subunit PMID:21525244 the mammalian orthologues named TRAPPC1, TRAPPC2, TRAPPC3, TRAPPC4, TRAPPC5, and TRAPPC6a/b |
| GO:0048193 Golgi vesicle transport | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: Golgi vesicle transport is a broad trafficking context supported by TRAPP/TRAPPII biology. Reason: Keep as non-core because it is broader/less precise than ER-to-Golgi vesicle-mediated transport and intra-Golgi TRAPPII context. Supporting Evidence: PMID:21453443 for distinct tethering events at Golgi membranes PMID:18930054 different functions in the regulation of ER-to-Golgi traffic |
| GO:0005515 protein binding | IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC3. Reason: Mark as over-annotated. Specific interaction biology should be represented as TRAPP/TRAPPII/TRAPPIII complex membership and TRAPP/RAB1 trafficking context, not generic GO:0005515. |
| GO:0005515 protein binding | IPI PMID:16828797 Structure of the Bet3-Tpc6B core of TRAPP: two Tpc6 paralogs... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC3. Reason: Mark as over-annotated. Specific interaction biology should be represented as TRAPP/TRAPPII/TRAPPIII complex membership and TRAPP/RAB1 trafficking context, not generic GO:0005515. |
| GO:0005515 protein binding | IPI PMID:16908848 Unique self-palmitoylation activity of the transport protein... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC3. Reason: Mark as over-annotated. Specific interaction biology should be represented as TRAPP/TRAPPII/TRAPPIII complex membership and TRAPP/RAB1 trafficking context, not generic GO:0005515. |
| GO:0005515 protein binding | IPI PMID:18930054 Distinct isocomplexes of the TRAPP trafficking factor coexis... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC3. Reason: Mark as over-annotated. Specific interaction biology should be represented as TRAPP/TRAPPII/TRAPPIII complex membership and TRAPP/RAB1 trafficking context, not generic GO:0005515. |
| GO:0005515 protein binding | IPI PMID:21453443 Organization and assembly of the TRAPPII complex. | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC3. Reason: Mark as over-annotated. Specific interaction biology should be represented as TRAPP/TRAPPII/TRAPPIII complex membership and TRAPP/RAB1 trafficking context, not generic GO:0005515. |
| GO:0005515 protein binding | IPI PMID:21827752 A yeast two hybrid screen identifies SPATA4 as a TRAPP inter... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC3. Reason: Mark as over-annotated. Specific interaction biology should be represented as TRAPP/TRAPPII/TRAPPIII complex membership and TRAPP/RAB1 trafficking context, not generic GO:0005515. |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC3. Reason: Mark as over-annotated. Specific interaction biology should be represented as TRAPP/TRAPPII/TRAPPIII complex membership and TRAPP/RAB1 trafficking context, not generic GO:0005515. |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC3. Reason: Mark as over-annotated. Specific interaction biology should be represented as TRAPP/TRAPPII/TRAPPIII complex membership and TRAPP/RAB1 trafficking context, not generic GO:0005515. |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC3. Reason: Mark as over-annotated. Specific interaction biology should be represented as TRAPP/TRAPPII/TRAPPIII complex membership and TRAPP/RAB1 trafficking context, not generic GO:0005515. |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC3. Reason: Mark as over-annotated. Specific interaction biology should be represented as TRAPP/TRAPPII/TRAPPIII complex membership and TRAPP/RAB1 trafficking context, not generic GO:0005515. |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC3. Reason: Mark as over-annotated. Specific interaction biology should be represented as TRAPP/TRAPPII/TRAPPIII complex membership and TRAPP/RAB1 trafficking context, not generic GO:0005515. |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC3. Reason: Mark as over-annotated. Specific interaction biology should be represented as TRAPP/TRAPPII/TRAPPIII complex membership and TRAPP/RAB1 trafficking context, not generic GO:0005515. |
| GO:0000139 Golgi membrane | IEA GO_REF:0000107 | ACCEPT | Summary: Golgi membrane localization is consistent with TRAPPC3/Bet3 role in TRAPP trafficking complexes. Reason: Accept as a more specific Golgi location for the TRAPP trafficking role. Supporting Evidence: PMID:21453443 for distinct tethering events at Golgi membranes PMID:18930054 different functions in the regulation of ER-to-Golgi traffic |
| GO:0005829 cytosol | IEA GO_REF:0000107 | ACCEPT | Summary: TRAPPC3-containing TRAPP reactions occur on the cytosolic/cytoplasmic side of ER-Golgi trafficking membranes. Reason: Accept as supported location for a peripheral trafficking-complex subunit. Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:0005737 cytoplasm | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | ACCEPT | Summary: TRAPPC3-containing TRAPP reactions occur on the cytosolic/cytoplasmic side of ER-Golgi trafficking membranes. Reason: Accept as supported location for a peripheral trafficking-complex subunit. Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | ACCEPT | Summary: TRAPPC3-containing TRAPP complexes mediate ER-to-Golgi vesicle traffic through TRAPP/RAB1 biology. Reason: Accept as a core process for TRAPPC3. Multiple human/mammalian TRAPP sources and Reactome support ER-to-Golgi trafficking. Supporting Evidence: PMID:18930054 The transport protein particle (TRAPP) complex is required for proper vesicular transport from the ER to the Golgi PMID:21525244 C4orf41 and TTC-15 (which we call TRAPPC11 and TRAPPC12, respectively) in ER-to-Golgi trafficking at a very early stage Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it |
| GO:0006901 vesicle coat assembly | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | MODIFY | Summary: TRAPPC3 interacts with COPII/SEC23-associated ER-derived vesicle trafficking machinery, but it is not a vesicle coat assembly factor. Reason: Modify to ER-to-Golgi vesicle-mediated transport to capture the supported trafficking role without overclaiming coat assembly. Proposed replacements: endoplasmic reticulum to Golgi vesicle-mediated transport Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a guanine-nucleotide exchange factor for RAB1 and is recruited to ER-derived vesicles by virtue of an interaction between the TRAPPC component TRAPPC3 and the coat protein SEC23 Reactome:R-HSA-8877475 TRAPPCII is recruited to ER-derived vesicles by virtue of an interaction between the TRAPPCII component TRAPPC3 and the COPII coat protein SEC23 |
| GO:0048208 COPII vesicle coat assembly | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | MODIFY | Summary: The COPII evidence supports TRAPPC3-SEC23 recruitment to ER-derived vesicles, not direct COPII coat assembly by TRAPPC3. Reason: Modify to ER-to-Golgi vesicle-mediated transport. Proposed replacements: endoplasmic reticulum to Golgi vesicle-mediated transport Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a guanine-nucleotide exchange factor for RAB1 and is recruited to ER-derived vesicles by virtue of an interaction between the TRAPPC component TRAPPC3 and the coat protein SEC23 Reactome:R-HSA-8877475 TRAPPCII is recruited to ER-derived vesicles by virtue of an interaction between the TRAPPCII component TRAPPC3 and the COPII coat protein SEC23 |
| GO:0099022 obsolete vesicle tethering | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | MODIFY | Summary: This obsolete vesicle-tethering annotation should not be kept as-is, and direct mammalian TRAPP tethering evidence remains unresolved. Reason: Modify to ER-to-Golgi vesicle-mediated transport, the safer supported TRAPPC3 process. Proposed replacements: endoplasmic reticulum to Golgi vesicle-mediated transport Supporting Evidence: PMID:27066478 evidence that any TRAPP complex acts as a membrane tether is currently inconclusive PMID:21525244 Direct demonstration of mammalian TRAPP as a vesicle tether has yet to be shown Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic |
| GO:1990071 TRAPPII protein complex | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | ACCEPT | Summary: TRAPPC3 is represented in TRAPPII complex models and ComplexPortal annotations. Reason: Accept as supported TRAPP subtype membership; process-level claims should remain tied to TRAPP/RAB1 trafficking. Supporting Evidence: PMID:21453443 for distinct tethering events at Golgi membranes Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:1990072 TRAPPIII protein complex | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | ACCEPT | Summary: TRAPPC3 is represented in TRAPPIII complex models, which are relevant to the PN autophagy-recruitment context. Reason: Accept as supported complex membership while keeping autophagy as context rather than assigning a broad autophagy BP. Supporting Evidence: Reactome:R-HSA-8877475 RAB1 and the TRAPPCIII complex play a role in the formation of the pre-autophagosomal structure (PAS) and contribute to the localization of ATG9 PMID:27066478 the connection of the mammalian TRAPP III complex to autophagy is currently not clear |
| GO:0005515 protein binding | IPI PMID:21525244 C4orf41 and TTC-15 are mammalian TRAPP components with a rol... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC3. Reason: Mark as over-annotated. Specific interaction biology should be represented as TRAPP/TRAPPII/TRAPPIII complex membership and TRAPP/RAB1 trafficking context, not generic GO:0005515. |
| GO:0030008 TRAPP complex | IDA PMID:21525244 C4orf41 and TTC-15 are mammalian TRAPP components with a rol... | ACCEPT | Summary: TRAPPC3/Bet3 is a core TRAPP complex subunit. Reason: Accept as core cellular-component membership. This is the cleanest gene-level representation of the PN TRAPP component leaf. Supporting Evidence: PMID:16828797 crystal structure of a human Bet3-Tpc6B heterodimer, which represents a core sub-complex in the assembly of TRAPP PMID:16828797 Bet3-Tpc6A and Bet3-Tpc6B, are able to recruit Mum2, a further TRAPP subunit PMID:21525244 the mammalian orthologues named TRAPPC1, TRAPPC2, TRAPPC3, TRAPPC4, TRAPPC5, and TRAPPC6a/b |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5694409 | ACCEPT | Summary: TRAPPC3-containing TRAPP reactions occur on the cytosolic/cytoplasmic side of ER-Golgi trafficking membranes. Reason: Accept as supported location for a peripheral trafficking-complex subunit. Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5694418 | ACCEPT | Summary: TRAPPC3-containing TRAPP reactions occur on the cytosolic/cytoplasmic side of ER-Golgi trafficking membranes. Reason: Accept as supported location for a peripheral trafficking-complex subunit. Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5694439 | ACCEPT | Summary: TRAPPC3-containing TRAPP reactions occur on the cytosolic/cytoplasmic side of ER-Golgi trafficking membranes. Reason: Accept as supported location for a peripheral trafficking-complex subunit. Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5694441 | ACCEPT | Summary: TRAPPC3-containing TRAPP reactions occur on the cytosolic/cytoplasmic side of ER-Golgi trafficking membranes. Reason: Accept as supported location for a peripheral trafficking-complex subunit. Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8877475 | ACCEPT | Summary: TRAPPC3-containing TRAPP reactions occur on the cytosolic/cytoplasmic side of ER-Golgi trafficking membranes. Reason: Accept as supported location for a peripheral trafficking-complex subunit. Supporting Evidence: Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
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Download this section (compressed HTML)Q: Should TRAPPC3 and related TRAPP core subunits retain contributes_to RAB1 GEF annotations even when the complex-level catalytic subunit assignment is not fully resolved?
Suggested experts: GO transport editors, Reactome TRAPP curators
Q: Should TRAPPIII complex membership be considered sufficient for PN autophagophore-recruitment context without assigning broad autophagy process terms?
Suggested experts: GO autophagy editors, ComplexPortal curators
Q: Should TRAPPC3 coat-assembly annotations be replaced by ER-to-Golgi vesicle-mediated transport or by a more specific TRAPPC/COPII recruitment term if one exists?
Suggested experts: GO transport editors, ComplexPortal curators
Experiment: Reconstitute TRAPPC3-containing TRAPP complexes and measure RAB1 GDP-GTP exchange with TRAPPC3 depletion/rescue or interface mutants.
Hypothesis: TRAPPC3 is required as a core TRAPP subunit for complex-level RAB1 GEF activity.
Type: complex reconstitution and RAB1 GEF assay
Experiment: Test TRAPPC3 mutants that disrupt TRAPPC6/TRAPPC1 interfaces for effects on ER-to-Golgi cargo trafficking, SEC23/COPII association, and Golgi membrane recruitment.
Hypothesis: TRAPPC3 scaffolding of core TRAPP interactions is required for ER-to-Golgi vesicle-mediated transport.
Type: trafficking rescue and complex-interface assay
Experiment: Separate TRAPPII and TRAPPIII pools in TRAPPC3 rescue cells and assay ATG9 localization plus canonical ER-to-Golgi transport in parallel.
Hypothesis: TRAPPC3 autophagy phenotypes depend on TRAPPIII/RAB1 context and should be distinguished from core ER-to-Golgi transport defects.
Type: TRAPP subtype-specific autophagy/traffic assay
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