TRAPPC5 is the human Trs31 ortholog and a small TRAPP core subunit. It supports TRAPP complex architecture, complex-level RAB1 guanine-nucleotide exchange, and ER-to-Golgi vesicle-mediated transport. Autophagy-related effects are best understood through TRAPP/TRAPPII/TRAPPIII complex membership and ER-Golgi trafficking rather than an independent TRAPPC5-specific autophagy function.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:1990071 TRAPPII protein complex | IBA GO_REF:0000033 | ACCEPT | Summary: TRAPPC5 is represented in TRAPPII protein complex through the shared TRAPP core. Reason: Accept as complex-context membership. Human TRAPPII/TRAPPIII are modeled as core TRAPP plus accessory subunits, and TRAPPC5 is a small core subunit. Supporting Evidence: PMID:21525244 with the mammalian orthologues named TRAPPC1, TRAPPC2, TRAPPC3, TRAPPC4, TRAPPC5, and TRAPPC6a/b PMID:27066478 TRAPP II, which contains core TRAPP plus TrappC9-10, and TRAPP III, which contains core TRAPP plus TrappC8, 11-13 Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:1990072 TRAPPIII protein complex | IBA GO_REF:0000033 | ACCEPT | Summary: TRAPPC5 is represented in TRAPPIII protein complex through the shared TRAPP core. Reason: Accept as complex-context membership. Human TRAPPII/TRAPPIII are modeled as core TRAPP plus accessory subunits, and TRAPPC5 is a small core subunit. Supporting Evidence: PMID:21525244 with the mammalian orthologues named TRAPPC1, TRAPPC2, TRAPPC3, TRAPPC4, TRAPPC5, and TRAPPC6a/b PMID:27066478 TRAPP II, which contains core TRAPP plus TrappC9-10, and TRAPP III, which contains core TRAPP plus TrappC8, 11-13 Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport | IBA GO_REF:0000033 | ACCEPT | Summary: TRAPPC5 contributes to TRAPP-dependent ER-to-Golgi trafficking. Reason: Accept as a core process. TRAPPC5 is a core TRAPP subunit, and TRAPP complex/RAB1 biology supports ER-to-Golgi vesicle-mediated transport. Supporting Evidence: PMID:21525244 in ER-to-Golgi trafficking at a very early stage Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it |
| GO:1990070 TRAPPI protein complex | IBA GO_REF:0000033 | MARK AS OVER ANNOTATED | Summary: TRAPPI protein complex is problematic as a human TRAPPC5 annotation. Reason: Mark as over-annotated. TRAPPC5 is a yeast TRAPP I core ortholog, but the mammalian literature questions the existence of a discrete TRAPP I complex in vivo. Supporting Evidence: PMID:21525244 absence of a TRAPP I-equivalent complex in mammalian cells PMID:27066478 TRAPP I cannot be separated from the mammalian TRAPP II and its existence in vivo in mammalian cells is currently in question PMID:27066478 there is currently no evidence for the existence of TRAPP I in mammalian cells |
| GO:0005783 endoplasmic reticulum | IEA GO_REF:0000044 | ACCEPT | Summary: endoplasmic reticulum localization is consistent with TRAPPC5 as a soluble/peripheral TRAPP trafficking subunit. Reason: Accept as a supported location for TRAPPC5-containing TRAPP reactions. Supporting Evidence: PMID:21525244 known human TRAPP components, including C3, C4, and C5 Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic |
| GO:0005794 Golgi apparatus | IEA GO_REF:0000044 | ACCEPT | Summary: Golgi apparatus localization is consistent with TRAPPC5 as a soluble/peripheral TRAPP trafficking subunit. Reason: Accept as a supported location for TRAPPC5-containing TRAPP reactions. Supporting Evidence: PMID:21525244 known human TRAPP components, including C3, C4, and C5 Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic |
| GO:0030008 TRAPP complex | IEA GO_REF:0000120 | ACCEPT | Summary: TRAPPC5 is a human TRAPP core subunit. Reason: Accept as core cellular-component membership. Mammalian TRAPP component work identifies C5/TRAPPC5 among known human TRAPP components. Supporting Evidence: PMID:21525244 with the mammalian orthologues named TRAPPC1, TRAPPC2, TRAPPC3, TRAPPC4, TRAPPC5, and TRAPPC6a/b PMID:21525244 known human TRAPP components, including C3, C4, and C5 PMID:27066478 A single TRAPP complex purified from mammalian cell lysate contains all the known subunits |
| GO:0048193 Golgi vesicle transport | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: Golgi vesicle transport is plausible TRAPP context but less specific than ER-to-Golgi transport for TRAPPC5. Reason: Keep as non-core. The core process is ER-to-Golgi/TRAPP/RAB1 transport; broader Golgi vesicle transport is a reasonable parent/context but not the most specific function. Supporting Evidence: PMID:21525244 in ER-to-Golgi trafficking at a very early stage Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC5 function. Reason: Mark as over-annotated. Proteome-scale interactions and TRAPP-subunit interactions should be interpreted through TRAPP complex membership, not generic protein binding. Supporting Evidence: PMID:21525244 known human TRAPP components, including C3, C4, and C5 |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC5 function. Reason: Mark as over-annotated. Proteome-scale interactions and TRAPP-subunit interactions should be interpreted through TRAPP complex membership, not generic protein binding. Supporting Evidence: PMID:21525244 known human TRAPP components, including C3, C4, and C5 |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC5 function. Reason: Mark as over-annotated. Proteome-scale interactions and TRAPP-subunit interactions should be interpreted through TRAPP complex membership, not generic protein binding. Supporting Evidence: PMID:21525244 known human TRAPP components, including C3, C4, and C5 |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC5 function. Reason: Mark as over-annotated. Proteome-scale interactions and TRAPP-subunit interactions should be interpreted through TRAPP complex membership, not generic protein binding. Supporting Evidence: PMID:21525244 known human TRAPP components, including C3, C4, and C5 |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MARK AS OVER ANNOTATED | Summary: Generic protein binding is not informative for TRAPPC5 function. Reason: Mark as over-annotated. Proteome-scale interactions and TRAPP-subunit interactions should be interpreted through TRAPP complex membership, not generic protein binding. Supporting Evidence: PMID:21525244 known human TRAPP components, including C3, C4, and C5 |
| GO:0005737 cytoplasm | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | ACCEPT | Summary: cytoplasm localization is consistent with TRAPPC5 as a soluble/peripheral TRAPP trafficking subunit. Reason: Accept as a supported location for TRAPPC5-containing TRAPP reactions. Supporting Evidence: PMID:21525244 known human TRAPP components, including C3, C4, and C5 Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic |
| GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | ACCEPT | Summary: TRAPPC5 contributes to TRAPP-dependent ER-to-Golgi trafficking. Reason: Accept as a core process. TRAPPC5 is a core TRAPP subunit, and TRAPP complex/RAB1 biology supports ER-to-Golgi vesicle-mediated transport. Supporting Evidence: PMID:21525244 in ER-to-Golgi trafficking at a very early stage Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it |
| GO:0006901 vesicle coat assembly | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | MODIFY | Summary: vesicle coat assembly overstates TRAPPC5 as a coat-assembly factor. Reason: Modify to ER-to-Golgi vesicle-mediated transport. TRAPPC5 participates in TRAPP-dependent trafficking, but the evidence does not show direct coat assembly by TRAPPC5. Proposed replacements: endoplasmic reticulum to Golgi vesicle-mediated transport Supporting Evidence: PMID:21525244 in ER-to-Golgi trafficking at a very early stage Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-8877475 TRAPPCII is recruited to ER-derived vesicles by virtue of an interaction between the TRAPPCII component TRAPPC3 and the COPII coat protein SEC23 |
| GO:0048208 COPII vesicle coat assembly | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | MODIFY | Summary: COPII vesicle coat assembly overstates TRAPPC5 as a coat-assembly factor. Reason: Modify to ER-to-Golgi vesicle-mediated transport. TRAPPC5 participates in TRAPP-dependent trafficking, but the evidence does not show direct coat assembly by TRAPPC5. Proposed replacements: endoplasmic reticulum to Golgi vesicle-mediated transport Supporting Evidence: PMID:21525244 in ER-to-Golgi trafficking at a very early stage Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-8877475 TRAPPCII is recruited to ER-derived vesicles by virtue of an interaction between the TRAPPCII component TRAPPC3 and the COPII coat protein SEC23 |
| GO:0099022 obsolete vesicle tethering | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | MODIFY | Summary: The obsolete vesicle-tethering annotation should not be kept as-is for TRAPPC5. Reason: Modify to ER-to-Golgi vesicle-mediated transport, the supported TRAPPC5/TRAPP process. Mammalian TRAPP tethering remains unresolved and the term is obsolete. Proposed replacements: endoplasmic reticulum to Golgi vesicle-mediated transport Supporting Evidence: PMID:27066478 evidence that any TRAPP complex acts as a membrane tether is currently inconclusive PMID:21525244 in ER-to-Golgi trafficking at a very early stage Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic |
| GO:1990071 TRAPPII protein complex | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | ACCEPT | Summary: TRAPPC5 is represented in TRAPPII protein complex through the shared TRAPP core. Reason: Accept as complex-context membership. Human TRAPPII/TRAPPIII are modeled as core TRAPP plus accessory subunits, and TRAPPC5 is a small core subunit. Supporting Evidence: PMID:21525244 with the mammalian orthologues named TRAPPC1, TRAPPC2, TRAPPC3, TRAPPC4, TRAPPC5, and TRAPPC6a/b PMID:27066478 TRAPP II, which contains core TRAPP plus TrappC9-10, and TRAPP III, which contains core TRAPP plus TrappC8, 11-13 Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:1990072 TRAPPIII protein complex | NAS PMID:27066478 TRAPP Complexes in Secretion and Autophagy. | ACCEPT | Summary: TRAPPC5 is represented in TRAPPIII protein complex through the shared TRAPP core. Reason: Accept as complex-context membership. Human TRAPPII/TRAPPIII are modeled as core TRAPP plus accessory subunits, and TRAPPC5 is a small core subunit. Supporting Evidence: PMID:21525244 with the mammalian orthologues named TRAPPC1, TRAPPC2, TRAPPC3, TRAPPC4, TRAPPC5, and TRAPPC6a/b PMID:27066478 TRAPP II, which contains core TRAPP plus TrappC9-10, and TRAPP III, which contains core TRAPP plus TrappC8, 11-13 Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:0030008 TRAPP complex | IDA PMID:21525244 C4orf41 and TTC-15 are mammalian TRAPP components with a rol... | ACCEPT | Summary: TRAPPC5 is a human TRAPP core subunit. Reason: Accept as core cellular-component membership. Mammalian TRAPP component work identifies C5/TRAPPC5 among known human TRAPP components. Supporting Evidence: PMID:21525244 with the mammalian orthologues named TRAPPC1, TRAPPC2, TRAPPC3, TRAPPC4, TRAPPC5, and TRAPPC6a/b PMID:21525244 known human TRAPP components, including C3, C4, and C5 PMID:27066478 A single TRAPP complex purified from mammalian cell lysate contains all the known subunits |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5694409 | ACCEPT | Summary: cytosol localization is consistent with TRAPPC5 as a soluble/peripheral TRAPP trafficking subunit. Reason: Accept as a supported location for TRAPPC5-containing TRAPP reactions. Supporting Evidence: Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5694418 | ACCEPT | Summary: cytosol localization is consistent with TRAPPC5 as a soluble/peripheral TRAPP trafficking subunit. Reason: Accept as a supported location for TRAPPC5-containing TRAPP reactions. Supporting Evidence: Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5694439 | ACCEPT | Summary: cytosol localization is consistent with TRAPPC5 as a soluble/peripheral TRAPP trafficking subunit. Reason: Accept as a supported location for TRAPPC5-containing TRAPP reactions. Supporting Evidence: Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:0005829 cytosol | TAS Reactome:R-HSA-5694441 | ACCEPT | Summary: cytosol localization is consistent with TRAPPC5 as a soluble/peripheral TRAPP trafficking subunit. Reason: Accept as a supported location for TRAPPC5-containing TRAPP reactions. Supporting Evidence: Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
| GO:0005829 cytosol | TAS Reactome:R-HSA-8877475 | ACCEPT | Summary: cytosol localization is consistent with TRAPPC5 as a soluble/peripheral TRAPP trafficking subunit. Reason: Accept as a supported location for TRAPPC5-containing TRAPP reactions. Supporting Evidence: Reactome:R-HSA-5694409 The TRAPPC complex acts as a guanine-nucleotide exchange factor for RAB1, activating it Reactome:R-HSA-5694439 TRAPPC is a multi-subunit tethering complex that facilitates ER-to-Golgi traffic Reactome:R-HSA-8877475 RAB1 nucleotide exchange is stimulated in these pathways by the GEF activity of the multisubunit TRAPPC complexes II and III |
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Download this section (compressed HTML)Q: Should TRAPPC5 receive a contributes_to RAB1 guanine-nucleotide exchange factor activity annotation based on Trs31-dependent yeast GEF evidence plus mammalian TRAPP complex models?
Suggested experts: GO transport editors, Reactome TRAPP curators
Q: Should human TRAPPI protein complex annotations be removed or replaced when the literature questions a discrete mammalian TRAPP I complex?
Suggested experts: GO cellular-component editors, ComplexPortal curators
Q: Should PN TRAPP-component autophagy context be represented only by TRAPPIII membership for TRAPPC5 unless TRAPPC5-specific autophagy experiments are available?
Suggested experts: GO autophagy editors, TRAPP biology experts
Experiment: Reconstitute TRAPPC5-containing human TRAPP complexes and measure RAB1 GDP-GTP exchange after TRAPPC5 depletion or interface mutation.
Hypothesis: TRAPPC5 is required as a core TRAPP subunit for complex-level RAB1 GEF activity.
Type: complex reconstitution and RAB1 GEF assay
Experiment: Use TRAPPC5 knockout/rescue cells to assay ER-to-Golgi cargo transport, TRAPP complex stability, and Golgi/ER localization of TRAPP subunits.
Hypothesis: TRAPPC5 loss disrupts TRAPP-dependent ER-to-Golgi vesicle-mediated transport by destabilizing or mislocalizing the TRAPP core.
Type: cellular trafficking rescue assay
Experiment: Biochemically separate human TRAPPII and TRAPPIII pools containing TRAPPC5 and test whether any discrete TRAPPI-like pool exists in vivo.
Hypothesis: TRAPPC5 is present in human TRAPPII/TRAPPIII assemblies, while a discrete mammalian TRAPPI complex is absent or not stable in vivo.
Type: complex purification and native fractionation
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