TSG101 encodes the UEV-domain ESCRT-I subunit that binds ubiquitin/PTAP-type motifs and scaffolds ESCRT-I with VPS28, VPS37-family subunits, and MVB12/UBAP-family subunits. Its conserved core role is ESCRT-I-mediated sorting of ubiquitinated endocytic cargo into multivesicular bodies and reverse-topology membrane fission. TSG101 also participates in ESCRT-I-dependent phagophore/autophagosome closure, best represented biologically as autophagosome assembly rather than generic macroautophagy. Viral budding, cytokinesis, exosome release, and transcription/cell-growth annotations are supported non-core or over-broad branches.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000813 ESCRT I complex | IBA GO_REF:0000033 | ACCEPT | Summary: ESCRT-I complex membership is the central TSG101 cellular-component annotation. Reason: TSG101 is a stoichiometric core ESCRT-I subunit with VPS28, VPS37-family subunits, and MVB12/UBAP-family subunits. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0008333 endosome to lysosome transport | IBA GO_REF:0000033 | ACCEPT | Summary: endosome to lysosome transport is supported as part of the core ESCRT-I endosomal cargo-sorting role. Reason: TSG101/ESCRT-I sorts ubiquitinated endocytic cargo into MVBs and thereby supports endosome-to-lysosome/vacuolar delivery of cargo. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt multivesicular bodies PMID:12900395 indirectly regulates multivesicular body formation PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0000813 ESCRT I complex | IEA GO_REF:0000117 | ACCEPT | Summary: ESCRT-I complex membership is the central TSG101 cellular-component annotation. Reason: TSG101 is a stoichiometric core ESCRT-I subunit with VPS28, VPS37-family subunits, and MVB12/UBAP-family subunits. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0005634 nucleus | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: nucleus localization is supported but is not the core proteostasis annotation. Reason: These locations reflect broad cytosolic/nuclear/midbody/plasma-membrane contexts for TSG101 and ESCRT-associated functions, but the core review focus is ESCRT-I endosomal sorting and phagophore closure. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Mainly cytoplasmic file:human/TSG101/TSG101-uniprot.txt Nuclear localization is cell cycle-dependent file:human/TSG101/TSG101-uniprot.txt localization to the midbody during cytokinesis |
| GO:0005737 cytoplasm | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: cytoplasm localization is supported but is not the core proteostasis annotation. Reason: These locations reflect broad cytosolic/nuclear/midbody/plasma-membrane contexts for TSG101 and ESCRT-associated functions, but the core review focus is ESCRT-I endosomal sorting and phagophore closure. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Mainly cytoplasmic file:human/TSG101/TSG101-uniprot.txt Nuclear localization is cell cycle-dependent file:human/TSG101/TSG101-uniprot.txt localization to the midbody during cytokinesis |
| GO:0005813 centrosome | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: centrosome localization is supported but is not the core proteostasis annotation. Reason: These locations reflect broad cytosolic/nuclear/midbody/plasma-membrane contexts for TSG101 and ESCRT-associated functions, but the core review focus is ESCRT-I endosomal sorting and phagophore closure. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Mainly cytoplasmic file:human/TSG101/TSG101-uniprot.txt Nuclear localization is cell cycle-dependent file:human/TSG101/TSG101-uniprot.txt localization to the midbody during cytokinesis |
| GO:0006355 regulation of DNA-templated transcription | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: regulation of DNA-templated transcription is a legacy/non-core nuclear transcription annotation and likely overstates the current functional picture. Reason: The synthesized evidence supports ESCRT-I ubiquitin/cargo sorting and membrane scission; DNA/transcription terms are not central and are not well integrated with the current proteostasis review. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt May be involved in cell growth and differentiation file:human/TSG101/TSG101-uniprot.txt Acts as a negative growth regulator |
| GO:0015031 protein transport | IEA GO_REF:0000002 | MODIFY | Summary: Generic protein transport is true but should be replaced by MVB/endosome-to-lysosome cargo sorting terms. Reason: The specific TSG101 transport role is ESCRT-I sorting of ubiquitinated cargo through MVB/endolysosomal pathways, not undifferentiated protein transport. Proposed replacements: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway multivesicular body assembly Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt multivesicular bodies PMID:12900395 indirectly regulates multivesicular body formation PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0016236 macroautophagy | IEA GO_REF:0000117 | MODIFY | Summary: macroautophagy is too broad or late-fusion-biased for the TSG101 autophagy role. Reason: The best-supported PN autophagy interpretation is ESCRT-I-mediated phagophore/autophagosome closure, which is better represented by autophagosome assembly. Proposed replacements: autophagosome assembly Supporting Evidence: PMID:31519728 VPS37A directs ESCRT recruitment for phagophore closure PMID:31519728 the core ESCRT-I components TSG101 and VPS28 PMID:31519728 required for autophagosome completion PMID:31519728 phagophore closure in mammalian cells PMID:31010855 mediates AP sealing PMID:31010855 ESCRT catalyzes AP closure |
| GO:0031901 early endosome membrane | IEA GO_REF:0000044 | ACCEPT | Summary: early endosome membrane localization is supported and relevant to ESCRT-I cargo sorting. Reason: TSG101 cycles between cytosol and active endosomal membranes, including early/late endosome and MVB contexts where ESCRT-I functions. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Early endosome membrane file:human/TSG101/TSG101-uniprot.txt Late endosome membrane PMID:12900395 Tsg101 to late endosomes PMID:12900395 colocalize on a subpopulation of endosomes |
| GO:0031902 late endosome membrane | IEA GO_REF:0000044 | ACCEPT | Summary: late endosome membrane localization is supported and relevant to ESCRT-I cargo sorting. Reason: TSG101 cycles between cytosol and active endosomal membranes, including early/late endosome and MVB contexts where ESCRT-I functions. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Early endosome membrane file:human/TSG101/TSG101-uniprot.txt Late endosome membrane PMID:12900395 Tsg101 to late endosomes PMID:12900395 colocalize on a subpopulation of endosomes |
| GO:0036258 multivesicular body assembly | IEA GO_REF:0000117 | ACCEPT | Summary: multivesicular body assembly is supported as part of the core ESCRT-I endosomal cargo-sorting role. Reason: TSG101/ESCRT-I sorts ubiquitinated endocytic cargo into MVBs and thereby supports endosome-to-lysosome/vacuolar delivery of cargo. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt multivesicular bodies PMID:12900395 indirectly regulates multivesicular body formation PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0039702 viral budding via host ESCRT complex | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: viral budding via host ESCRT complex is supported but reflects host-pathogen exploitation of ESCRT-I. Reason: Viral budding/release annotations are well supported for TSG101 but are non-core for the human proteostasis-network review. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Involved in the budding of many viruses file:human/TSG101/TSG101-uniprot.txt interaction is essential for viral particle budding PMID:11595185 required for HIV-1 budding PMID:11595185 arrests HIV-1 budding at a late stage |
| GO:0045892 negative regulation of DNA-templated transcription | IEA GO_REF:0000108 | MARK AS OVER ANNOTATED | Summary: negative regulation of DNA-templated transcription is a legacy/non-core nuclear transcription annotation and likely overstates the current functional picture. Reason: The synthesized evidence supports ESCRT-I ubiquitin/cargo sorting and membrane scission; DNA/transcription terms are not central and are not well integrated with the current proteostasis review. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt May be involved in cell growth and differentiation file:human/TSG101/TSG101-uniprot.txt Acts as a negative growth regulator |
| GO:0090543 Flemming body | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Flemming body localization is supported but is not the core proteostasis annotation. Reason: These locations reflect broad cytosolic/nuclear/midbody/plasma-membrane contexts for TSG101 and ESCRT-associated functions, but the core review focus is ESCRT-I endosomal sorting and phagophore closure. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Mainly cytoplasmic file:human/TSG101/TSG101-uniprot.txt Nuclear localization is cell cycle-dependent file:human/TSG101/TSG101-uniprot.txt localization to the midbody during cytokinesis |
| GO:0005515 protein binding | IPI PMID:12802020 TSG101 interaction with HRS mediates endosomal trafficking a... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:12900395 Hrs regulates multivesicular body formation via ESCRT recrui... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:15033475 Physical and functional interactions between Daxx and TSG101... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:15611048 Interactions of TOM1L1 with the multivesicular body sorting ... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:16118794 SIMPLE interacts with NEDD4 and TSG101: evidence for a role ... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:16189514 Towards a proteome-scale map of the human protein-protein in... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:17174262 HD-PTP and Alix share some membrane-traffic related proteins... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:17350572 Structural and biochemical studies of ALIX/AIP1 and its role... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:17853893 Human ESCRT and ALIX proteins interact with proteins of the ... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:19060904 An empirical framework for binary interactome mapping. | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:19549727 Analysis of the human E2 ubiquitin conjugating enzyme protei... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:20176808 TEX14 interacts with CEP55 to block cell abscission. | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:26871637 Widespread Expansion of Protein Interaction Capabilities by ... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:27107012 Pooled-matrix protein interaction screens using Barcode Fusi... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:35044719 Proteome-scale mapping of binding sites in the unstructured ... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:37100772 Large-scale phage-based screening reveals extensive pan-vira... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:37219487 Large-scale phosphomimetic screening identifies phospho-modu... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:39526800 A comprehensive two-hybrid analysis to explore the Legionell... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:40205054 Multimodal cell maps as a foundation for structural and func... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0003714 transcription corepressor activity | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: transcription corepressor activity is a legacy/non-core nuclear transcription annotation and likely overstates the current functional picture. Reason: The synthesized evidence supports ESCRT-I ubiquitin/cargo sorting and membrane scission; DNA/transcription terms are not central and are not well integrated with the current proteostasis review. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt May be involved in cell growth and differentiation file:human/TSG101/TSG101-uniprot.txt Acts as a negative growth regulator |
| GO:0046755 viral budding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: viral budding is supported but reflects host-pathogen exploitation of ESCRT-I. Reason: Viral budding/release annotations are well supported for TSG101 but are non-core for the human proteostasis-network review. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Involved in the budding of many viruses file:human/TSG101/TSG101-uniprot.txt interaction is essential for viral particle budding PMID:11595185 required for HIV-1 budding PMID:11595185 arrests HIV-1 budding at a late stage |
| GO:0070062 extracellular exosome | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Extracellular exosome detection is supported but is a non-core localization/context. Reason: TSG101 is commonly detected in exosome preparations and regulates exosomal release, but this is secondary to its ESCRT-I membrane-remodeling role. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Required for the exosomal release of SDCBP, CD63 and syndecan file:human/TSG101/TSG101-uniprot.txt extracellular release of microvesicles |
| GO:1990182 exosomal secretion | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: exosomal secretion is supported but non-core in this PN review. Reason: TSG101 contributes to exosome/microvesicle release, but the proteostasis core here is ESCRT-I endosomal cargo sorting and autophagophore closure. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Required for the exosomal release of SDCBP, CD63 and syndecan file:human/TSG101/TSG101-uniprot.txt extracellular release of microvesicles |
| GO:2000397 positive regulation of ubiquitin-dependent endocytosis | IEA GO_REF:0000107 | ACCEPT | Summary: positive regulation of ubiquitin-dependent endocytosis is supported as a cargo-sorting consequence of ESCRT-I function. Reason: TSG101-mediated ESCRT-I sorting of ubiquitinated receptors into the MVB/lysosomal pathway explains EGFR/endocytosis down-regulation annotations. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt multivesicular bodies PMID:12900395 indirectly regulates multivesicular body formation PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0005829 cytosol | IDA GO_REF:0000052 | KEEP AS NON CORE | Summary: cytosol localization is supported but is not the core proteostasis annotation. Reason: These locations reflect broad cytosolic/nuclear/midbody/plasma-membrane contexts for TSG101 and ESCRT-associated functions, but the core review focus is ESCRT-I endosomal sorting and phagophore closure. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Mainly cytoplasmic file:human/TSG101/TSG101-uniprot.txt Nuclear localization is cell cycle-dependent file:human/TSG101/TSG101-uniprot.txt localization to the midbody during cytokinesis |
| GO:0005634 nucleus | EXP PMID:17229889 Spongiform neurodegeneration-associated E3 ligase Mahogunin ... | KEEP AS NON CORE | Summary: nucleus localization is supported but is not the core proteostasis annotation. Reason: These locations reflect broad cytosolic/nuclear/midbody/plasma-membrane contexts for TSG101 and ESCRT-associated functions, but the core review focus is ESCRT-I endosomal sorting and phagophore closure. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Mainly cytoplasmic file:human/TSG101/TSG101-uniprot.txt Nuclear localization is cell cycle-dependent file:human/TSG101/TSG101-uniprot.txt localization to the midbody during cytokinesis |
| GO:0005737 cytoplasm | EXP PMID:17853893 Human ESCRT and ALIX proteins interact with proteins of the ... | KEEP AS NON CORE | Summary: cytoplasm localization is supported but is not the core proteostasis annotation. Reason: These locations reflect broad cytosolic/nuclear/midbody/plasma-membrane contexts for TSG101 and ESCRT-associated functions, but the core review focus is ESCRT-I endosomal sorting and phagophore closure. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Mainly cytoplasmic file:human/TSG101/TSG101-uniprot.txt Nuclear localization is cell cycle-dependent file:human/TSG101/TSG101-uniprot.txt localization to the midbody during cytokinesis |
| GO:0031901 early endosome membrane | EXP PMID:23166352 Charcot-Marie-Tooth disease-linked protein SIMPLE functions ... | ACCEPT | Summary: early endosome membrane localization is supported and relevant to ESCRT-I cargo sorting. Reason: TSG101 cycles between cytosol and active endosomal membranes, including early/late endosome and MVB contexts where ESCRT-I functions. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Early endosome membrane file:human/TSG101/TSG101-uniprot.txt Late endosome membrane PMID:12900395 Tsg101 to late endosomes PMID:12900395 colocalize on a subpopulation of endosomes |
| GO:0031902 late endosome membrane | EXP PMID:11916981 Mammalian class E vps proteins recognize ubiquitin and act i... | ACCEPT | Summary: late endosome membrane localization is supported and relevant to ESCRT-I cargo sorting. Reason: TSG101 cycles between cytosol and active endosomal membranes, including early/late endosome and MVB contexts where ESCRT-I functions. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Early endosome membrane file:human/TSG101/TSG101-uniprot.txt Late endosome membrane PMID:12900395 Tsg101 to late endosomes PMID:12900395 colocalize on a subpopulation of endosomes |
| GO:0031902 late endosome membrane | EXP PMID:17229889 Spongiform neurodegeneration-associated E3 ligase Mahogunin ... | ACCEPT | Summary: late endosome membrane localization is supported and relevant to ESCRT-I cargo sorting. Reason: TSG101 cycles between cytosol and active endosomal membranes, including early/late endosome and MVB contexts where ESCRT-I functions. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Early endosome membrane file:human/TSG101/TSG101-uniprot.txt Late endosome membrane PMID:12900395 Tsg101 to late endosomes PMID:12900395 colocalize on a subpopulation of endosomes |
| GO:0061631 ubiquitin conjugating enzyme activity | IKR NOT PMID:9241264 TSG101 may be the prototype of a class of dominant negative ... | ACCEPT | Summary: The negated ubiquitin-conjugating-enzyme annotation is correct for TSG101. Reason: This is a NOT annotation. TSG101 is a UEV-domain protein that binds ubiquitin/PTAP motifs but should not be curated as an active catalytic ubiquitin-conjugating enzyme. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt The UEV domain binds ubiquitin file:human/TSG101/TSG101-uniprot.txt P-[ST]-A-P peptide motif independently file:human/TSG101/TSG101-uniprot.txt Belongs to the ubiquitin-conjugating enzyme family. UEV |
| GO:0000813 ESCRT I complex | IPI PMID:18005716 Identification of human MVB12 proteins as ESCRT-I subunits t... | ACCEPT | Summary: ESCRT-I complex membership is the central TSG101 cellular-component annotation. Reason: TSG101 is a stoichiometric core ESCRT-I subunit with VPS28, VPS37-family subunits, and MVB12/UBAP-family subunits. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0000813 ESCRT I complex | IPI PMID:21757351 UBAP1 is a component of an endosome-specific ESCRT-I complex... | ACCEPT | Summary: ESCRT-I complex membership is the central TSG101 cellular-component annotation. Reason: TSG101 is a stoichiometric core ESCRT-I subunit with VPS28, VPS37-family subunits, and MVB12/UBAP-family subunits. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0000813 ESCRT I complex | IPI PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo... | ACCEPT | Summary: ESCRT-I complex membership is the central TSG101 cellular-component annotation. Reason: TSG101 is a stoichiometric core ESCRT-I subunit with VPS28, VPS37-family subunits, and MVB12/UBAP-family subunits. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0010008 endosome membrane | NAS PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo... | ACCEPT | Summary: endosome membrane localization is supported and relevant to ESCRT-I cargo sorting. Reason: TSG101 cycles between cytosol and active endosomal membranes, including early/late endosome and MVB contexts where ESCRT-I functions. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Early endosome membrane file:human/TSG101/TSG101-uniprot.txt Late endosome membrane PMID:12900395 Tsg101 to late endosomes PMID:12900395 colocalize on a subpopulation of endosomes |
| GO:0036258 multivesicular body assembly | NAS PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo... | ACCEPT | Summary: multivesicular body assembly is supported as part of the core ESCRT-I endosomal cargo-sorting role. Reason: TSG101/ESCRT-I sorts ubiquitinated endocytic cargo into MVBs and thereby supports endosome-to-lysosome/vacuolar delivery of cargo. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt multivesicular bodies PMID:12900395 indirectly regulates multivesicular body formation PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | NAS PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo... | ACCEPT | Summary: protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway is supported as part of the core ESCRT-I endosomal cargo-sorting role. Reason: TSG101/ESCRT-I sorts ubiquitinated endocytic cargo into MVBs and thereby supports endosome-to-lysosome/vacuolar delivery of cargo. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt multivesicular bodies PMID:12900395 indirectly regulates multivesicular body formation PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0090148 membrane fission | NAS PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo... | ACCEPT | Summary: Membrane fission is supported as the shared ESCRT reverse-topology membrane-remodeling activity. Reason: ESCRT-I participates upstream of ESCRT-III/VPS4-driven neck scission in MVB formation, cytokinesis/viral budding, and phagophore closure contexts. Supporting Evidence: PMID:20588296 ESCRT-I and ESCRT-II direct membrane budding away from the cytosol PMID:31519728 ESCRT-mediated membrane scission in phagophore closure PMID:31519728 the core ESCRT-I components TSG101 and VPS28 |
| GO:0005515 protein binding | IPI PMID:15218037 The human endosomal sorting complex required for transport (... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:1903774 positive regulation of viral budding via host ESCRT complex | IMP PMID:11595185 Tsg101 and the vacuolar protein sorting pathway are essentia... | KEEP AS NON CORE | Summary: positive regulation of viral budding via host ESCRT complex is supported but reflects host-pathogen exploitation of ESCRT-I. Reason: Viral budding/release annotations are well supported for TSG101 but are non-core for the human proteostasis-network review. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Involved in the budding of many viruses file:human/TSG101/TSG101-uniprot.txt interaction is essential for viral particle budding PMID:11595185 required for HIV-1 budding PMID:11595185 arrests HIV-1 budding at a late stage |
| GO:0005515 protein binding | IPI PMID:18005716 Identification of human MVB12 proteins as ESCRT-I subunits t... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0046755 viral budding | IMP PMID:18005716 Identification of human MVB12 proteins as ESCRT-I subunits t... | KEEP AS NON CORE | Summary: viral budding is supported but reflects host-pathogen exploitation of ESCRT-I. Reason: Viral budding/release annotations are well supported for TSG101 but are non-core for the human proteostasis-network review. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Involved in the budding of many viruses file:human/TSG101/TSG101-uniprot.txt interaction is essential for viral particle budding PMID:11595185 required for HIV-1 budding PMID:11595185 arrests HIV-1 budding at a late stage |
| GO:0005515 protein binding | IPI PMID:22315426 Formation and release of arrestin domain-containing protein ... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0006858 extracellular transport | IMP PMID:22315426 Formation and release of arrestin domain-containing protein ... | KEEP AS NON CORE | Summary: extracellular transport is supported but non-core in this PN review. Reason: TSG101 contributes to exosome/microvesicle release, but the proteostasis core here is ESCRT-I endosomal cargo sorting and autophagophore closure. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Required for the exosomal release of SDCBP, CD63 and syndecan file:human/TSG101/TSG101-uniprot.txt extracellular release of microvesicles |
| GO:0007175 negative regulation of epidermal growth factor-activated receptor activity | IMP PMID:16973552 Human ESCRT-II complex and its role in human immunodeficienc... | ACCEPT | Summary: negative regulation of epidermal growth factor-activated receptor activity is supported as a cargo-sorting consequence of ESCRT-I function. Reason: TSG101-mediated ESCRT-I sorting of ubiquitinated receptors into the MVB/lysosomal pathway explains EGFR/endocytosis down-regulation annotations. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt multivesicular bodies PMID:12900395 indirectly regulates multivesicular body formation PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0044877 protein-containing complex binding | IDA PMID:16973552 Human ESCRT-II complex and its role in human immunodeficienc... | MARK AS OVER ANNOTATED | Summary: protein-containing complex binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:18641129 Differential requirements for Alix and ESCRT-III in cytokine... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0016236 macroautophagy | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | MODIFY | Summary: macroautophagy is too broad or late-fusion-biased for the TSG101 autophagy role. Reason: The best-supported PN autophagy interpretation is ESCRT-I-mediated phagophore/autophagosome closure, which is better represented by autophagosome assembly. Proposed replacements: autophagosome assembly Supporting Evidence: PMID:31519728 VPS37A directs ESCRT recruitment for phagophore closure PMID:31519728 the core ESCRT-I components TSG101 and VPS28 PMID:31519728 required for autophagosome completion PMID:31519728 phagophore closure in mammalian cells PMID:31010855 mediates AP sealing PMID:31010855 ESCRT catalyzes AP closure |
| GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway | IMP PMID:16554368 The ESCRT-III subunit hVps24 is required for degradation but... | ACCEPT | Summary: negative regulation of epidermal growth factor receptor signaling pathway is supported as a cargo-sorting consequence of ESCRT-I function. Reason: TSG101-mediated ESCRT-I sorting of ubiquitinated receptors into the MVB/lysosomal pathway explains EGFR/endocytosis down-regulation annotations. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt multivesicular bodies PMID:12900395 indirectly regulates multivesicular body formation PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0000813 ESCRT I complex | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | ACCEPT | Summary: ESCRT-I complex membership is the central TSG101 cellular-component annotation. Reason: TSG101 is a stoichiometric core ESCRT-I subunit with VPS28, VPS37-family subunits, and MVB12/UBAP-family subunits. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0036258 multivesicular body assembly | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | ACCEPT | Summary: multivesicular body assembly is supported as part of the core ESCRT-I endosomal cargo-sorting role. Reason: TSG101/ESCRT-I sorts ubiquitinated endocytic cargo into MVBs and thereby supports endosome-to-lysosome/vacuolar delivery of cargo. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt multivesicular bodies PMID:12900395 indirectly regulates multivesicular body formation PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0039702 viral budding via host ESCRT complex | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | KEEP AS NON CORE | Summary: viral budding via host ESCRT complex is supported but reflects host-pathogen exploitation of ESCRT-I. Reason: Viral budding/release annotations are well supported for TSG101 but are non-core for the human proteostasis-network review. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Involved in the budding of many viruses file:human/TSG101/TSG101-uniprot.txt interaction is essential for viral particle budding PMID:11595185 required for HIV-1 budding PMID:11595185 arrests HIV-1 budding at a late stage |
| GO:0000813 ESCRT I complex | TAS PMID:21118109 The role of ESCRT proteins in fusion events involving lysoso... | ACCEPT | Summary: ESCRT-I complex membership is the central TSG101 cellular-component annotation. Reason: TSG101 is a stoichiometric core ESCRT-I subunit with VPS28, VPS37-family subunits, and MVB12/UBAP-family subunits. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0097352 autophagosome maturation | TAS PMID:21118109 The role of ESCRT proteins in fusion events involving lysoso... | MODIFY | Summary: autophagosome maturation is too broad or late-fusion-biased for the TSG101 autophagy role. Reason: The best-supported PN autophagy interpretation is ESCRT-I-mediated phagophore/autophagosome closure, which is better represented by autophagosome assembly. Proposed replacements: autophagosome assembly Supporting Evidence: PMID:31519728 VPS37A directs ESCRT recruitment for phagophore closure PMID:31519728 the core ESCRT-I components TSG101 and VPS28 PMID:31519728 required for autophagosome completion PMID:31519728 phagophore closure in mammalian cells PMID:31010855 mediates AP sealing PMID:31010855 ESCRT catalyzes AP closure |
| GO:0043405 regulation of MAP kinase activity | IMP PMID:17714434 Vps22/EAP30 in ESCRT-II mediates endosomal sorting of growth... | KEEP AS NON CORE | Summary: MAP kinase regulation is a downstream receptor-signaling consequence and not core. Reason: This likely reflects altered EGFR trafficking/signaling downstream of ESCRT function rather than a direct TSG101 molecular role. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt multivesicular bodies PMID:12900395 indirectly regulates multivesicular body formation PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0005515 protein binding | IPI PMID:16973552 Human ESCRT-II complex and its role in human immunodeficienc... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0019076 viral release from host cell | IMP PMID:11595185 Tsg101 and the vacuolar protein sorting pathway are essentia... | KEEP AS NON CORE | Summary: viral release from host cell is supported but reflects host-pathogen exploitation of ESCRT-I. Reason: Viral budding/release annotations are well supported for TSG101 but are non-core for the human proteostasis-network review. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Involved in the budding of many viruses file:human/TSG101/TSG101-uniprot.txt interaction is essential for viral particle budding PMID:11595185 required for HIV-1 budding PMID:11595185 arrests HIV-1 budding at a late stage |
| GO:0043130 ubiquitin binding | IDA PMID:11595185 Tsg101 and the vacuolar protein sorting pathway are essentia... | ACCEPT | Summary: Ubiquitin binding is a core TSG101 UEV-domain molecular function. Reason: TSG101 binds ubiquitin/ubiquitinated cargo through its UEV domain and uses that activity in ESCRT-I cargo sorting and PTAP-motif recruitment contexts. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin file:human/TSG101/TSG101-uniprot.txt The UEV domain binds ubiquitin PMID:11595185 UEV domain of Tsg101 binds PMID:11595185 to ubiquitin |
| GO:0046790 virion binding | IDA PMID:11595185 Tsg101 and the vacuolar protein sorting pathway are essentia... | KEEP AS NON CORE | Summary: virion binding is supported but reflects host-pathogen exploitation of ESCRT-I. Reason: Viral budding/release annotations are well supported for TSG101 but are non-core for the human proteostasis-network review. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Involved in the budding of many viruses file:human/TSG101/TSG101-uniprot.txt interaction is essential for viral particle budding PMID:11595185 required for HIV-1 budding PMID:11595185 arrests HIV-1 budding at a late stage |
| GO:0000813 ESCRT I complex | IDA PMID:18005716 Identification of human MVB12 proteins as ESCRT-I subunits t... | ACCEPT | Summary: ESCRT-I complex membership is the central TSG101 cellular-component annotation. Reason: TSG101 is a stoichiometric core ESCRT-I subunit with VPS28, VPS37-family subunits, and MVB12/UBAP-family subunits. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0005768 endosome | IDA PMID:17940959 Involvement of vacuolar protein sorting pathway in Ebola vir... | ACCEPT | Summary: endosome localization is supported and relevant to ESCRT-I cargo sorting. Reason: TSG101 cycles between cytosol and active endosomal membranes, including early/late endosome and MVB contexts where ESCRT-I functions. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Early endosome membrane file:human/TSG101/TSG101-uniprot.txt Late endosome membrane PMID:12900395 Tsg101 to late endosomes PMID:12900395 colocalize on a subpopulation of endosomes |
| GO:0005886 plasma membrane | IDA PMID:17940959 Involvement of vacuolar protein sorting pathway in Ebola vir... | KEEP AS NON CORE | Summary: plasma membrane localization is supported but is not the core proteostasis annotation. Reason: These locations reflect broad cytosolic/nuclear/midbody/plasma-membrane contexts for TSG101 and ESCRT-associated functions, but the core review focus is ESCRT-I endosomal sorting and phagophore closure. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Mainly cytoplasmic file:human/TSG101/TSG101-uniprot.txt Nuclear localization is cell cycle-dependent file:human/TSG101/TSG101-uniprot.txt localization to the midbody during cytokinesis |
| GO:1903774 positive regulation of viral budding via host ESCRT complex | IMP PMID:15218037 The human endosomal sorting complex required for transport (... | KEEP AS NON CORE | Summary: positive regulation of viral budding via host ESCRT complex is supported but reflects host-pathogen exploitation of ESCRT-I. Reason: Viral budding/release annotations are well supported for TSG101 but are non-core for the human proteostasis-network review. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Involved in the budding of many viruses file:human/TSG101/TSG101-uniprot.txt interaction is essential for viral particle budding PMID:11595185 required for HIV-1 budding PMID:11595185 arrests HIV-1 budding at a late stage |
| GO:0019076 viral release from host cell | IMP PMID:15218037 The human endosomal sorting complex required for transport (... | KEEP AS NON CORE | Summary: viral release from host cell is supported but reflects host-pathogen exploitation of ESCRT-I. Reason: Viral budding/release annotations are well supported for TSG101 but are non-core for the human proteostasis-network review. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Involved in the budding of many viruses file:human/TSG101/TSG101-uniprot.txt interaction is essential for viral particle budding PMID:11595185 required for HIV-1 budding PMID:11595185 arrests HIV-1 budding at a late stage |
| GO:0005769 early endosome | IDA PMID:11916981 Mammalian class E vps proteins recognize ubiquitin and act i... | ACCEPT | Summary: early endosome localization is supported and relevant to ESCRT-I cargo sorting. Reason: TSG101 cycles between cytosol and active endosomal membranes, including early/late endosome and MVB contexts where ESCRT-I functions. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Early endosome membrane file:human/TSG101/TSG101-uniprot.txt Late endosome membrane PMID:12900395 Tsg101 to late endosomes PMID:12900395 colocalize on a subpopulation of endosomes |
| GO:0043130 ubiquitin binding | IDA PMID:11916981 Mammalian class E vps proteins recognize ubiquitin and act i... | ACCEPT | Summary: Ubiquitin binding is a core TSG101 UEV-domain molecular function. Reason: TSG101 binds ubiquitin/ubiquitinated cargo through its UEV domain and uses that activity in ESCRT-I cargo sorting and PTAP-motif recruitment contexts. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin file:human/TSG101/TSG101-uniprot.txt The UEV domain binds ubiquitin PMID:11595185 UEV domain of Tsg101 binds PMID:11595185 to ubiquitin |
| GO:1903551 regulation of extracellular exosome assembly | IMP PMID:24105262 Analysis of ESCRT functions in exosome biogenesis, compositi... | KEEP AS NON CORE | Summary: regulation of extracellular exosome assembly is supported but non-core in this PN review. Reason: TSG101 contributes to exosome/microvesicle release, but the proteostasis core here is ESCRT-I endosomal cargo sorting and autophagophore closure. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Required for the exosomal release of SDCBP, CD63 and syndecan file:human/TSG101/TSG101-uniprot.txt extracellular release of microvesicles |
| GO:1903543 positive regulation of exosomal secretion | IMP PMID:22660413 Syndecan-syntenin-ALIX regulates the biogenesis of exosomes. | KEEP AS NON CORE | Summary: positive regulation of exosomal secretion is supported but non-core in this PN review. Reason: TSG101 contributes to exosome/microvesicle release, but the proteostasis core here is ESCRT-I endosomal cargo sorting and autophagophore closure. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Required for the exosomal release of SDCBP, CD63 and syndecan file:human/TSG101/TSG101-uniprot.txt extracellular release of microvesicles |
| GO:1903543 positive regulation of exosomal secretion | IMP PMID:24105262 Analysis of ESCRT functions in exosome biogenesis, compositi... | KEEP AS NON CORE | Summary: positive regulation of exosomal secretion is supported but non-core in this PN review. Reason: TSG101 contributes to exosome/microvesicle release, but the proteostasis core here is ESCRT-I endosomal cargo sorting and autophagophore closure. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Required for the exosomal release of SDCBP, CD63 and syndecan file:human/TSG101/TSG101-uniprot.txt extracellular release of microvesicles |
| GO:0070062 extracellular exosome | IDA PMID:23092844 Monitoring the Rab27 associated exosome pathway using nanopa... | KEEP AS NON CORE | Summary: Extracellular exosome detection is supported but is a non-core localization/context. Reason: TSG101 is commonly detected in exosome preparations and regulates exosomal release, but this is secondary to its ESCRT-I membrane-remodeling role. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Required for the exosomal release of SDCBP, CD63 and syndecan file:human/TSG101/TSG101-uniprot.txt extracellular release of microvesicles |
| GO:0070062 extracellular exosome | HDA PMID:23533145 In-depth proteomic analyses of exosomes isolated from expres... | KEEP AS NON CORE | Summary: Extracellular exosome detection is supported but is a non-core localization/context. Reason: TSG101 is commonly detected in exosome preparations and regulates exosomal release, but this is secondary to its ESCRT-I membrane-remodeling role. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Required for the exosomal release of SDCBP, CD63 and syndecan file:human/TSG101/TSG101-uniprot.txt extracellular release of microvesicles |
| GO:0070062 extracellular exosome | IDA PMID:16501490 Collection, storage, preservation, and normalization of huma... | KEEP AS NON CORE | Summary: Extracellular exosome detection is supported but is a non-core localization/context. Reason: TSG101 is commonly detected in exosome preparations and regulates exosomal release, but this is secondary to its ESCRT-I membrane-remodeling role. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Required for the exosomal release of SDCBP, CD63 and syndecan file:human/TSG101/TSG101-uniprot.txt extracellular release of microvesicles |
| GO:0005515 protein binding | IPI PMID:14519844 Divergent retroviral late-budding domains recruit vacuolar p... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0042803 protein homodimerization activity | IPI PMID:14519844 Divergent retroviral late-budding domains recruit vacuolar p... | KEEP AS NON CORE | Summary: Homodimerization is supported as a structural interaction but is not the core functional annotation. Reason: Retain this as a non-core interaction property; ESCRT-I complex membership and ubiquitin binding capture the main TSG101 role. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Self-associates |
| GO:0005515 protein binding | IPI PMID:14505570 The protein network of HIV budding. | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0000813 ESCRT I complex | IDA PMID:20654576 Distinct functions of human MVB12A and MVB12B in the ESCRT-I... | ACCEPT | Summary: ESCRT-I complex membership is the central TSG101 cellular-component annotation. Reason: TSG101 is a stoichiometric core ESCRT-I subunit with VPS28, VPS37-family subunits, and MVB12/UBAP-family subunits. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0000813 ESCRT I complex | IDA PMID:22232651 Structural basis for membrane targeting by the MVB12-associa... | ACCEPT | Summary: ESCRT-I complex membership is the central TSG101 cellular-component annotation. Reason: TSG101 is a stoichiometric core ESCRT-I subunit with VPS28, VPS37-family subunits, and MVB12/UBAP-family subunits. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0043130 ubiquitin binding | IDA PMID:20654576 Distinct functions of human MVB12A and MVB12B in the ESCRT-I... | ACCEPT | Summary: Ubiquitin binding is a core TSG101 UEV-domain molecular function. Reason: TSG101 binds ubiquitin/ubiquitinated cargo through its UEV domain and uses that activity in ESCRT-I cargo sorting and PTAP-motif recruitment contexts. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin file:human/TSG101/TSG101-uniprot.txt The UEV domain binds ubiquitin PMID:11595185 UEV domain of Tsg101 binds PMID:11595185 to ubiquitin |
| GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | IC PMID:20654576 Distinct functions of human MVB12A and MVB12B in the ESCRT-I... | ACCEPT | Summary: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway is supported as part of the core ESCRT-I endosomal cargo-sorting role. Reason: TSG101/ESCRT-I sorts ubiquitinated endocytic cargo into MVBs and thereby supports endosome-to-lysosome/vacuolar delivery of cargo. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt multivesicular bodies PMID:12900395 indirectly regulates multivesicular body formation PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0070062 extracellular exosome | HDA PMID:19056867 Large-scale proteomics and phosphoproteomics of urinary exos... | KEEP AS NON CORE | Summary: Extracellular exosome detection is supported but is a non-core localization/context. Reason: TSG101 is commonly detected in exosome preparations and regulates exosomal release, but this is secondary to its ESCRT-I membrane-remodeling role. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Required for the exosomal release of SDCBP, CD63 and syndecan file:human/TSG101/TSG101-uniprot.txt extracellular release of microvesicles |
| GO:0010008 endosome membrane | TAS Reactome:R-HSA-184269 | ACCEPT | Summary: endosome membrane localization is supported and relevant to ESCRT-I cargo sorting. Reason: TSG101 cycles between cytosol and active endosomal membranes, including early/late endosome and MVB contexts where ESCRT-I functions. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Early endosome membrane file:human/TSG101/TSG101-uniprot.txt Late endosome membrane PMID:12900395 Tsg101 to late endosomes PMID:12900395 colocalize on a subpopulation of endosomes |
| GO:0010008 endosome membrane | TAS Reactome:R-HSA-3149434 | ACCEPT | Summary: endosome membrane localization is supported and relevant to ESCRT-I cargo sorting. Reason: TSG101 cycles between cytosol and active endosomal membranes, including early/late endosome and MVB contexts where ESCRT-I functions. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Early endosome membrane file:human/TSG101/TSG101-uniprot.txt Late endosome membrane PMID:12900395 Tsg101 to late endosomes PMID:12900395 colocalize on a subpopulation of endosomes |
| GO:0010008 endosome membrane | TAS Reactome:R-HSA-3159232 | ACCEPT | Summary: endosome membrane localization is supported and relevant to ESCRT-I cargo sorting. Reason: TSG101 cycles between cytosol and active endosomal membranes, including early/late endosome and MVB contexts where ESCRT-I functions. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Early endosome membrane file:human/TSG101/TSG101-uniprot.txt Late endosome membrane PMID:12900395 Tsg101 to late endosomes PMID:12900395 colocalize on a subpopulation of endosomes |
| GO:0010008 endosome membrane | TAS Reactome:R-HSA-917696 | ACCEPT | Summary: endosome membrane localization is supported and relevant to ESCRT-I cargo sorting. Reason: TSG101 cycles between cytosol and active endosomal membranes, including early/late endosome and MVB contexts where ESCRT-I functions. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Early endosome membrane file:human/TSG101/TSG101-uniprot.txt Late endosome membrane PMID:12900395 Tsg101 to late endosomes PMID:12900395 colocalize on a subpopulation of endosomes |
| GO:0010008 endosome membrane | TAS Reactome:R-HSA-917730 | ACCEPT | Summary: endosome membrane localization is supported and relevant to ESCRT-I cargo sorting. Reason: TSG101 cycles between cytosol and active endosomal membranes, including early/late endosome and MVB contexts where ESCRT-I functions. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Early endosome membrane file:human/TSG101/TSG101-uniprot.txt Late endosome membrane PMID:12900395 Tsg101 to late endosomes PMID:12900395 colocalize on a subpopulation of endosomes |
| GO:0070062 extracellular exosome | IDA PMID:15326289 Identification and proteomic profiling of exosomes in human ... | KEEP AS NON CORE | Summary: Extracellular exosome detection is supported but is a non-core localization/context. Reason: TSG101 is commonly detected in exosome preparations and regulates exosomal release, but this is secondary to its ESCRT-I membrane-remodeling role. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Required for the exosomal release of SDCBP, CD63 and syndecan file:human/TSG101/TSG101-uniprot.txt extracellular release of microvesicles |
| GO:0000813 ESCRT I complex | IDA PMID:21757351 UBAP1 is a component of an endosome-specific ESCRT-I complex... | ACCEPT | Summary: ESCRT-I complex membership is the central TSG101 cellular-component annotation. Reason: TSG101 is a stoichiometric core ESCRT-I subunit with VPS28, VPS37-family subunits, and MVB12/UBAP-family subunits. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | IMP PMID:21757351 UBAP1 is a component of an endosome-specific ESCRT-I complex... | ACCEPT | Summary: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway is supported as part of the core ESCRT-I endosomal cargo-sorting role. Reason: TSG101/ESCRT-I sorts ubiquitinated endocytic cargo into MVBs and thereby supports endosome-to-lysosome/vacuolar delivery of cargo. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt multivesicular bodies PMID:12900395 indirectly regulates multivesicular body formation PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0031625 ubiquitin protein ligase binding | IPI PMID:17229889 Spongiform neurodegeneration-associated E3 ligase Mahogunin ... | KEEP AS NON CORE | Summary: Ubiquitin ligase binding is supported as regulatory context but not core. Reason: TSG101 interacts with LRSAM1/MGRN1 and can be monoubiquitinated, but this regulatory binding is secondary to ESCRT-I cargo sorting. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Interacts with LRSAM1 file:human/TSG101/TSG101-uniprot.txt Interacts with MGRN1 file:human/TSG101/TSG101-uniprot.txt Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1 |
| GO:0005769 early endosome | IDA PMID:17229889 Spongiform neurodegeneration-associated E3 ligase Mahogunin ... | ACCEPT | Summary: early endosome localization is supported and relevant to ESCRT-I cargo sorting. Reason: TSG101 cycles between cytosol and active endosomal membranes, including early/late endosome and MVB contexts where ESCRT-I functions. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Early endosome membrane file:human/TSG101/TSG101-uniprot.txt Late endosome membrane PMID:12900395 Tsg101 to late endosomes PMID:12900395 colocalize on a subpopulation of endosomes |
| GO:0005515 protein binding | IPI PMID:18256029 Identification of Alix-type and Non-Alix-type ALG-2-binding ... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0048306 calcium-dependent protein binding | IPI PMID:19520058 Penta-EF-hand protein ALG-2 functions as a Ca2+-dependent ad... | MARK AS OVER ANNOTATED | Summary: calcium-dependent protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005515 protein binding | IPI PMID:18077552 Regulation of Tsg101 expression by the steadiness box: a rol... | MARK AS OVER ANNOTATED | Summary: protein binding is too generic to represent TSG101 function. Reason: The interaction evidence should be represented by ubiquitin binding, ESCRT-I complex membership, MVB sorting, viral budding context, or cytokinesis context rather than generic binding terms. Proposed replacements: ubiquitin binding ESCRT I complex Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Component of the ESCRT-I complex file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt which consists of TSG101, VPS28, a VPS37 protein PMID:12900395 ESCRT-I subunit Tsg101 PMID:12900395 the initial recruitment of ESCRT-I to endosomes file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin |
| GO:0005737 cytoplasm | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: cytoplasm localization is supported but is not the core proteostasis annotation. Reason: These locations reflect broad cytosolic/nuclear/midbody/plasma-membrane contexts for TSG101 and ESCRT-associated functions, but the core review focus is ESCRT-I endosomal sorting and phagophore closure. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Mainly cytoplasmic file:human/TSG101/TSG101-uniprot.txt Nuclear localization is cell cycle-dependent file:human/TSG101/TSG101-uniprot.txt localization to the midbody during cytokinesis |
| GO:0005737 cytoplasm | IDA PMID:18077552 Regulation of Tsg101 expression by the steadiness box: a rol... | KEEP AS NON CORE | Summary: cytoplasm localization is supported but is not the core proteostasis annotation. Reason: These locations reflect broad cytosolic/nuclear/midbody/plasma-membrane contexts for TSG101 and ESCRT-associated functions, but the core review focus is ESCRT-I endosomal sorting and phagophore closure. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Mainly cytoplasmic file:human/TSG101/TSG101-uniprot.txt Nuclear localization is cell cycle-dependent file:human/TSG101/TSG101-uniprot.txt localization to the midbody during cytokinesis |
| GO:0046755 viral budding | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: viral budding is supported but reflects host-pathogen exploitation of ESCRT-I. Reason: Viral budding/release annotations are well supported for TSG101 but are non-core for the human proteostasis-network review. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Involved in the budding of many viruses file:human/TSG101/TSG101-uniprot.txt interaction is essential for viral particle budding PMID:11595185 required for HIV-1 budding PMID:11595185 arrests HIV-1 budding at a late stage |
| GO:0005770 late endosome | IMP PMID:15126635 Nedd4.1-mediated ubiquitination and subsequent recruitment o... | ACCEPT | Summary: late endosome localization is supported and relevant to ESCRT-I cargo sorting. Reason: TSG101 cycles between cytosol and active endosomal membranes, including early/late endosome and MVB contexts where ESCRT-I functions. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Early endosome membrane file:human/TSG101/TSG101-uniprot.txt Late endosome membrane PMID:12900395 Tsg101 to late endosomes PMID:12900395 colocalize on a subpopulation of endosomes |
| GO:0046755 viral budding | IMP PMID:15126635 Nedd4.1-mediated ubiquitination and subsequent recruitment o... | KEEP AS NON CORE | Summary: viral budding is supported but reflects host-pathogen exploitation of ESCRT-I. Reason: Viral budding/release annotations are well supported for TSG101 but are non-core for the human proteostasis-network review. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Involved in the budding of many viruses file:human/TSG101/TSG101-uniprot.txt interaction is essential for viral particle budding PMID:11595185 required for HIV-1 budding PMID:11595185 arrests HIV-1 budding at a late stage |
| GO:0005771 multivesicular body | TAS PMID:15611048 Interactions of TOM1L1 with the multivesicular body sorting ... | ACCEPT | Summary: multivesicular body localization is supported and relevant to ESCRT-I cargo sorting. Reason: TSG101 cycles between cytosol and active endosomal membranes, including early/late endosome and MVB contexts where ESCRT-I functions. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Early endosome membrane file:human/TSG101/TSG101-uniprot.txt Late endosome membrane PMID:12900395 Tsg101 to late endosomes PMID:12900395 colocalize on a subpopulation of endosomes |
| GO:0043130 ubiquitin binding | TAS PMID:15611048 Interactions of TOM1L1 with the multivesicular body sorting ... | ACCEPT | Summary: Ubiquitin binding is a core TSG101 UEV-domain molecular function. Reason: TSG101 binds ubiquitin/ubiquitinated cargo through its UEV domain and uses that activity in ESCRT-I cargo sorting and PTAP-motif recruitment contexts. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt Binds to ubiquitinated cargo proteins file:human/TSG101/TSG101-uniprot.txt Interacts with ubiquitin file:human/TSG101/TSG101-uniprot.txt The UEV domain binds ubiquitin PMID:11595185 UEV domain of Tsg101 binds PMID:11595185 to ubiquitin |
| GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | IDA PMID:15611048 Interactions of TOM1L1 with the multivesicular body sorting ... | ACCEPT | Summary: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway is supported as part of the core ESCRT-I endosomal cargo-sorting role. Reason: TSG101/ESCRT-I sorts ubiquitinated endocytic cargo into MVBs and thereby supports endosome-to-lysosome/vacuolar delivery of cargo. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt required for the sorting of endocytic ubiquitinated cargos into file:human/TSG101/TSG101-uniprot.txt multivesicular bodies PMID:12900395 indirectly regulates multivesicular body formation PMID:12900395 the initial recruitment of ESCRT-I to endosomes |
| GO:0003677 DNA binding | TAS PMID:10888872 DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a c... | MARK AS OVER ANNOTATED | Summary: DNA binding is a legacy/non-core nuclear transcription annotation and likely overstates the current functional picture. Reason: The synthesized evidence supports ESCRT-I ubiquitin/cargo sorting and membrane scission; DNA/transcription terms are not central and are not well integrated with the current proteostasis review. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt May be involved in cell growth and differentiation file:human/TSG101/TSG101-uniprot.txt Acts as a negative growth regulator |
| GO:0003714 transcription corepressor activity | TAS PMID:10888872 DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a c... | MARK AS OVER ANNOTATED | Summary: transcription corepressor activity is a legacy/non-core nuclear transcription annotation and likely overstates the current functional picture. Reason: The synthesized evidence supports ESCRT-I ubiquitin/cargo sorting and membrane scission; DNA/transcription terms are not central and are not well integrated with the current proteostasis review. Supporting Evidence: file:human/TSG101/TSG101-uniprot.txt May be involved in cell growth and differentiation file:human/TSG101/TSG101-uniprot.txt Acts as a negative growth regulator |
| GO:0000045 autophagosome assembly | IMP PMID:31519728 VPS37A directs ESCRT recruitment for phagophore closure. | NEW | Summary: TSG101 should be added to autophagosome assembly for the ESCRT-I phagophore-closure role. Reason: The PN leaf is autophagophore sealing, and mammalian evidence shows core ESCRT-I components including TSG101 are required for autophagosome completion/phagophore closure. Supporting Evidence: PMID:31519728 VPS37A directs ESCRT recruitment for phagophore closure PMID:31519728 the core ESCRT-I components TSG101 and VPS28 PMID:31519728 required for autophagosome completion PMID:31519728 phagophore closure in mammalian cells PMID:31010855 mediates AP sealing PMID:31010855 ESCRT catalyzes AP closure |
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Download this section (compressed HTML)Q: Should the TSG101 autophagy annotation be changed from broad macroautophagy/autophagosome maturation to GO:0000045 autophagosome assembly for phagophore closure?
Suggested experts: GO autophagy editors, GO ESCRT curators
Q: Should generic TSG101 protein-binding annotations be retired in favor of ubiquitin binding, ESCRT-I complex membership, and specific pathway contexts?
Suggested experts: GO molecular function editors, UniProt curators
Q: Should viral budding, cytokinesis, and exosome annotations remain non-core branches in proteostasis-focused TSG101 curation?
Suggested experts: GO viral process editors, GO cell-cycle editors, GO extracellular vesicle editors
Experiment: Use acute TSG101 depletion/rescue with UEV-domain and VPS28/VPS37-binding mutants, then measure HT-LC3 closure, EGFR MVB sorting, and ESCRT-III/VPS4 recruitment side by side.
Hypothesis: TSG101 UEV-domain ubiquitin/PTAP-motif binding and ESCRT-I assembly surfaces make separable contributions to phagophore closure and MVB cargo sorting.
Type: TSG101 separation-of-function phagophore closure rescue
Experiment: Endogenously tag TSG101, VPS28, VPS37A, CHMP2A, and LC3 reporters and quantify recruitment order during starvation-induced phagophore closure.
Hypothesis: Core ESCRT-I components including TSG101 transiently localize to closing LC3-positive phagophores before ESCRT-III/VPS4 recruitment.
Type: endogenous ESCRT-I autophagy recruitment imaging
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