ID   TXD16_HUMAN             Reviewed;         825 AA.
AC   Q9P2K2; A5PKW9; A7E260; A7MD07; B9EH67; Q9H9W7;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   04-NOV-2008, sequence version 4.
DT   10-JUN-2026, entry version 165.
DE   RecName: Full=Thioredoxin domain-containing protein 16 {ECO:0000312|HGNC:HGNC:19965};
DE   Flags: Precursor;
GN   Name=TXNDC16 {ECO:0000312|HGNC:HGNC:19965};
GN   Synonyms=ERP90 {ECO:0000303|PubMed:21359175}, KIAA1344;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT LYS-486.
RC   TISSUE=Brain;
RX   PubMed=10718198; DOI=10.1093/dnares/7.1.65;
RA   Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.;
RT   "Prediction of the coding sequences of unidentified human genes. XVI. The
RT   complete sequences of 150 new cDNA clones from brain which code for large
RT   proteins in vitro.";
RL   DNA Res. 7:65-73(2000).
RN   [2]
RP   SEQUENCE REVISION.
RX   PubMed=12168954; DOI=10.1093/dnares/9.3.99;
RA   Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.;
RT   "Construction of expression-ready cDNA clones for KIAA genes: manual
RT   curation of 330 KIAA cDNA clones.";
RL   DNA Res. 9:99-106(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT LYS-486.
RC   TISSUE=Lung, and Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 456-825.
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [6]
RP   SUBCELLULAR LOCATION, INTERACTION WITH FOXRED2, DISULFIDE BONDS, AND
RP   GLYCOSYLATION.
RX   PubMed=21359175; DOI=10.1371/journal.pone.0017037;
RA   Riemer J., Hansen H.G., Appenzeller-Herzog C., Johansson L., Ellgaard L.;
RT   "Identification of the PDI-family member ERp90 as an interaction partner of
RT   ERFAD.";
RL   PLoS ONE 6:E17037-E17037(2011).
RN   [7]
RP   SUBCELLULAR LOCATION.
RX   PubMed=25122923; DOI=10.4049/jimmunol.1303098;
RA   Harz C., Ludwig N., Lang S., Werner T.V., Galata V., Backes C., Schmitt K.,
RA   Nickels R., Krause E., Jung M., Rettig J., Keller A., Menger M.,
RA   Zimmermann R., Meese E.;
RT   "Secretion and immunogenicity of the meningioma-associated antigen
RT   TXNDC16.";
RL   J. Immunol. 193:3146-3154(2014).
CC   -!- SUBUNIT: Interacts with FOXRED2. {ECO:0000269|PubMed:21359175}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:25122923}.
CC       Endoplasmic reticulum lumen {ECO:0000269|PubMed:21359175,
CC       ECO:0000269|PubMed:25122923}.
CC   -!- DOMAIN: Contains a masked and non-functional KDEL endoplasmic reticulum
CC       retrieval motif. {ECO:0000269|PubMed:25122923}.
CC   -!- PTM: Glycosylated. {ECO:0000269|PubMed:21359175}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB14101.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AB037765; BAA92582.2; -; mRNA.
DR   EMBL; CH471078; EAW65650.1; -; Genomic_DNA.
DR   EMBL; BC137081; AAI37082.1; -; mRNA.
DR   EMBL; BC137082; AAI37083.1; -; mRNA.
DR   EMBL; BC142650; AAI42651.1; -; mRNA.
DR   EMBL; BC150205; AAI50206.1; -; mRNA.
DR   EMBL; BC152428; AAI52429.1; -; mRNA.
DR   EMBL; AK022563; BAB14101.1; ALT_INIT; mRNA.
DR   CCDS; CCDS32083.1; -.
DR   RefSeq; NP_001153519.1; NM_001160047.1.
DR   RefSeq; NP_065835.2; NM_020784.3.
DR   AlphaFoldDB; Q9P2K2; -.
DR   SMR; Q9P2K2; -.
DR   BioGRID; 121602; 63.
DR   FunCoup; Q9P2K2; 728.
DR   IntAct; Q9P2K2; 53.
DR   STRING; 9606.ENSP00000281741; -.
DR   GlyCosmos; Q9P2K2; 1 site, No reported glycans.
DR   GlyGen; Q9P2K2; 1 site, 3 N-linked glycans (1 site).
DR   iPTMnet; Q9P2K2; -.
DR   PhosphoSitePlus; Q9P2K2; -.
DR   BioMuta; TXNDC16; -.
DR   DMDM; 212276524; -.
DR   jPOST; Q9P2K2; -.
DR   MassIVE; Q9P2K2; -.
DR   PaxDb; 9606-ENSP00000281741; -.
DR   PeptideAtlas; Q9P2K2; -.
DR   ProteomicsDB; 83828; -.
DR   Pumba; Q9P2K2; -.
DR   Antibodypedia; 149; 36 antibodies from 15 providers.
DR   DNASU; 57544; -.
DR   Ensembl; ENST00000281741.9; ENSP00000281741.4; ENSG00000087301.11.
DR   Ensembl; ENST00000936707.1; ENSP00000606766.1; ENSG00000087301.11.
DR   Ensembl; ENST00000956219.1; ENSP00000626278.1; ENSG00000087301.11.
DR   GeneID; 57544; -.
DR   KEGG; hsa:57544; -.
DR   MANE-Select; ENST00000281741.9; ENSP00000281741.4; NM_020784.3; NP_065835.2.
DR   UCSC; uc001wzs.4; human.
DR   AGR; HGNC:19965; -.
DR   ClinPGx; PA162407488; -.
DR   CTD; 57544; -.
DR   GeneCards; TXNDC16; -.
DR   HGNC; HGNC:19965; TXNDC16.
DR   HPA; ENSG00000087301; Low tissue specificity.
DR   MIM; 616179; gene.
DR   OpenTargets; ENSG00000087301; -.
DR   VEuPathDB; HostDB:ENSG00000087301; -.
DR   eggNOG; KOG0191; Eukaryota.
DR   GeneTree; ENSGT00390000006080; -.
DR   HOGENOM; CLU_018100_1_0_1; -.
DR   InParanoid; Q9P2K2; -.
DR   OMA; CRRTLMG; -.
DR   OrthoDB; 427280at2759; -.
DR   PAN-GO; Q9P2K2; 0 GO annotations based on evolutionary models.
DR   PhylomeDB; Q9P2K2; -.
DR   PathwayCommons; Q9P2K2; -.
DR   SignaLink; Q9P2K2; -.
DR   Agora; ENSG00000087301; -.
DR   BioGRID-ORCS; 57544; 10 hits in 1152 CRISPR screens.
DR   ChiTaRS; TXNDC16; human.
DR   GenomeRNAi; 57544; -.
DR   Pharos; Q9P2K2; Tdark.
DR   PRO; PR:Q9P2K2; -.
DR   Proteomes; UP000005640; Chromosome 14.
DR   RNAct; Q9P2K2; protein.
DR   Bgee; ENSG00000087301; Expressed in seminal vesicle and 179 other cell types or tissues.
DR   ExpressionAtlas; Q9P2K2; baseline and differential.
DR   GO; GO:0005788; C:endoplasmic reticulum lumen; IDA:UniProtKB.
DR   GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR   CDD; cd02961; PDI_a_family; 1.
DR   FunFam; 3.40.30.10:FF:000226; Thioredoxin domain containing 16; 1.
DR   Gene3D; 3.40.30.10; Glutaredoxin; 1.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   InterPro; IPR013766; Thioredoxin_domain.
DR   InterPro; IPR040090; TXNDC16.
DR   InterPro; IPR057642; TXNDC16_2nd.
DR   InterPro; IPR057645; TXNDC16_3rd.
DR   InterPro; IPR057639; TXNDC16_N.
DR   PANTHER; PTHR22699; THIOREDOXIN DOMAIN-CONTAINING PROTEIN 16; 1.
DR   PANTHER; PTHR22699:SF1; THIOREDOXIN DOMAIN-CONTAINING PROTEIN 16; 1.
DR   Pfam; PF00085; Thioredoxin; 1.
DR   Pfam; PF13848; Thioredoxin_6; 1.
DR   Pfam; PF24509; TXNDC16_2nd; 1.
DR   Pfam; PF24510; TXNDC16_3rd; 1.
DR   Pfam; PF24508; TXNDC16_N; 1.
DR   SUPFAM; SSF52833; Thioredoxin-like; 1.
PE   1: Evidence at protein level;
KW   Disulfide bond; Endoplasmic reticulum; Glycoprotein;
KW   Proteomics identification; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255, ECO:0000269|PubMed:25122923"
FT   CHAIN           28..825
FT                   /note="Thioredoxin domain-containing protein 16"
FT                   /id="PRO_0000257789"
FT   DOMAIN          392..495
FT                   /note="Thioredoxin"
FT   REGION          762..787
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           816..819
FT                   /note="Mediates endoplasmic reticulum retention"
FT                   /evidence="ECO:0000269|PubMed:25122923"
FT   COMPBIAS        767..787
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        460
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        449..456
FT                   /evidence="ECO:0000269|PubMed:21359175"
FT   VARIANT         136
FT                   /note="S -> N (in dbSNP:rs28759013)"
FT                   /id="VAR_061899"
FT   VARIANT         152
FT                   /note="N -> Y (in dbSNP:rs28593180)"
FT                   /id="VAR_061900"
FT   VARIANT         486
FT                   /note="E -> K (in dbSNP:rs7155490)"
FT                   /evidence="ECO:0000269|PubMed:10718198,
FT                   ECO:0000269|PubMed:15489334"
FT                   /id="VAR_028919"
FT   CONFLICT        180
FT                   /note="A -> G (in Ref. 1; BAA92582)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   825 AA;  93572 MW;  B86F6DA47B5098D6 CRC64;
     MFSGFNVFRV GISFVIMCIF YMPTVNSLPE LSPQKYFSTL QPGKASLAYF CQADSPRTSV
     FLEELNEAVR PLQDYGISVA KVNCVKEEIS RYCGKEKDLM KAYLFKGNIL LREFPTDTLF
     DVNAIVAHVL FALLFSEVKY ITNLEDLQNI ENALKGKANI IFSYVRAIGI PEHRAVMEAA
     FVYGTTYQFV LTTEIALLES IGSEDVEYAH LYFFHCKLVL DLTQQCRRTL MEQPLTTLNI
     HLFIKTMKAP LLTEVAEDPQ QVSTVHLQLG LPLVFIVSQQ ATYEADRRTA EWVAWRLLGK
     AGVLLLLRDS LEVNIPQDAN VVFKRAEEGV PVEFLVLHDV DLIISHVENN MHIEEIQEDE
     DNDMEGPDID VQDDEVAETV FRDRKRKLPL ELTVELTEET FNATVMASDS IVLFYAGWQA
     VSMAFLQSYI DVAVKLKGTS TMLLTRINCA DWSDVCTKQN VTEFPIIKMY KKGENPVSYA
     GMLGTEDLLK FIQLNRISYP VNITSIQEAE EYLSGELYKD LILYSSVSVL GLFSPTMKTA
     KEDFSEAGNY LKGYVITGIY SEEDVLLLST KYAASLPALL LARHTEGKIE SIPLASTHAQ
     DIVQIITDAL LEMFPEITVE NLPSYFRLQK PLLILFSDGT VNPQYKKAIL TLVKQKYLDS
     FTPCWLNLKN TPVGRGILRA YFDPLPPLPL LVLVNLHSGG QVFAFPSDQA IIEENLVLWL
     KKLEAGLENH ITILPAQEWK PPLPAYDFLS MIDAATSQRG TRKVPKCMKE TDVQENDKEQ
     HEDKSAVRKE PIETLRIKHW NRSNWFKEAE KSFRRDKELG CSKVN
//