id: F8WDQ9
gene_symbol: UBE2F
taxon:
  id: NCBITaxon:9606
  label: Homo sapiens
status: COMPLETE
description: The short UBE2F product lacks the catalytic cysteine and most of the E2 core. Intrinsic ubiquitin
  transfer is unsupported and contradicted by sequence and NEDD8 specificity; indirect ubiquitination
  involvement cannot be established for this product.
source_documents:
- genes/human/UBE2F/UBE2F-protnlm-source.json
- genes/human/UBE2F/UBE2F-notes.md
references:
- id: PMID:19250909
  title: E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.
  findings:
  - statement: Biochemical and cellular experiments identify full-length UBE2F as a NEDD8-conjugating
      E2, not a ubiquitin E2.
    supporting_text: 'the previously uncharacterized E2 UBE2F is a NEDD8-conjugating enzyme in vitro

      and in vivo.'
  full_text_unavailable: true
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: Primary report checked against the cached abstract/full text; the evidence scope is
      stated in the finding.
- id: PMID:23300442
  title: Inhibition of a NEDD8 Cascade Restores Restriction of HIV by APOBEC3G.
  findings:
  - statement: Full-length UBE2F indirectly enables a ubiquitin-ligase pathway through cullin neddylation;
      this is distinct from intrinsic ubiquitin transfer.
    supporting_text: 'conjugation of NEDD8 to Cullin-5 by the NEDD8-conjugating

      enzyme UBE2F is required for HIV Vif-mediated degradation'
  full_text_unavailable: false
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: Primary report checked against the cached abstract/full text; the evidence scope is
      stated in the finding.
- id: file:human/UBE2F/UBE2F-bioinformatics/RESULTS.md
  title: Exact UBE2F product-to-reference sequence mapping
- id: file:human/UBE2F/UBE2F-protnlm-source.json
  title: Frozen exact-input ProtNLM output and UniProt sequence for F8WDQ9
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-10
  source_reference_id: file:human/UBE2F/UBE2F-protnlm-source.json
  predicted_term:
    id: GO:0004842
    label: ubiquitin-protein transferase activity
  predicted_term_type: GO_MF
  review:
    assessment: NPI
    error_type: DOMAIN_ARCHITECTURE_MISMATCH
    confidence_score: 0
    summary: F8WDQ9 lacks the reference UBE2F catalytic Cys116 and a complete UBC core. Moreover, the
      characterized full-length enzyme conjugates NEDD8, not ubiquitin. A UBC-domain match does not establish
      ubiquitin-protein transferase activity.
    supported_by:
    - &id001
      reference_id: file:human/UBE2F/UBE2F-bioinformatics/RESULTS.md
      supporting_text: '| Active site: Glycyl thioester intermediate | 116–116 | 0 / 1 | 0 |'
    - reference_id: PMID:19250909
      supporting_text: 'the previously uncharacterized E2 UBE2F is a NEDD8-conjugating enzyme in vitro

        and in vivo.'
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-10
  source_reference_id: file:human/UBE2F/UBE2F-protnlm-source.json
  predicted_term:
    id: GO:0016567
    label: protein ubiquitination
  predicted_term_type: GO_BP
  review:
    assessment: UNC
    confidence_score: 1
    summary: Full-length UBE2F supports CUL5-dependent ubiquitination through NEDD8 conjugation, so this
      biological process is not intrinsically incompatible with the gene. The 101-residue selected product
      lacks the catalytic E2 site and has no demonstrated assembly or regulatory function; its participation
      in protein ubiquitination is unresolved.
    supported_by:
    - *id001
    - reference_id: PMID:23300442
      supporting_text: 'conjugation of NEDD8 to Cullin-5 by the NEDD8-conjugating

        enzyme UBE2F is required for HIV Vif-mediated degradation'