id: Q92900 gene_symbol: UPF1 product_type: PROTEIN status: COMPLETE taxon: id: NCBITaxon:9606 label: Homo sapiens alternative_products: - name: '1' id: Q92900-1 - name: '2' id: Q92900-2 sequence_note: VSP_003393 description: UPF1 (regulator of nonsense transcripts 1; RENT1) is the central ATP-dependent RNA helicase and ATPase of the nonsense-mediated mRNA decay (NMD) pathway. A superfamily-1 (SF1) helicase with an N-terminal cysteine-histidine-rich (CH) zinc-binding regulatory domain, UPF1 is recruited to mRNAs at translation termination, where release factors (eRF1/eRF3) and the SMG1 kinase complex assemble the transient SURF complex. In EJC-dependent NMD the adaptor UPF2 bridges UPF1 to UPF3 and the downstream exon-junction complex, forming the UPF1-UPF2-UPF3 surveillance complex; UPF1 is then phosphorylated by SMG1 and the phospho-form is recognized by SMG5/SMG6/SMG7 to recruit decay enzymes and PP2A. UPF1 ATPase/helicase activity remodels and disassembles target mRNPs, committing premature-termination-codon-containing and certain long-3'UTR or staufen-bound transcripts to degradation, and also mediates replication-dependent histone mRNA decay at the end of S phase. UPF1 acts mainly on cytoplasmic translating mRNAs (with a smaller nuclear pool), concentrates in P-bodies in its hyperphosphorylated state, and has additional reported moonlighting roles at telomeres and in DNA replication; it can unwind both RNA and DNA in vitro. existing_annotations: - term: id: GO:0003724 label: RNA helicase activity evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: enables review: summary: RNA helicase activity is the core catalytic molecular function of UPF1, the central ATP-dependent helicase of nonsense-mediated mRNA decay. action: ACCEPT reason: Directly and repeatedly supported; UPF1 is an RNA-dependent helicase required for NMD. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: RNA-dependent helicase required for nonsense-mediated decay - term: id: GO:0000184 label: nuclear-transcribed mRNA catabolic process, nonsense-mediated decay evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: involved_in review: summary: Nonsense-mediated mRNA decay of PTC-containing transcripts is the central biological process of UPF1; it is recruited at termination and triggers decay. action: ACCEPT reason: Core biological process; UPF1 is the central NMD factor. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: recruited to mRNAs upon translation termination - term: id: GO:0000932 label: P-body evidence_type: IEA original_reference_id: GO_REF:0000044 qualifier: located_in review: summary: P-body localization; hyperphosphorylated UPF1 is targeted to P-bodies where mRNA decay factors concentrate. action: KEEP_AS_NON_CORE reason: Supported by UniProt note that the hyperphosphorylated form is targeted to P-bodies; a functional but non-core localization. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: Hyperphosphorylated form is - term: id: GO:0003677 label: DNA binding evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: enables review: summary: DNA binding (IEA); UPF1 can act on DNA substrates in vitro and has reported telomere/replication roles. action: KEEP_AS_NON_CORE reason: Supported in vitro but peripheral to the core RNA/NMD function. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: Also capable of unwinding double-stranded DNA and - term: id: GO:0003723 label: RNA binding evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: enables review: summary: RNA binding underlies UPF1 recruitment to target mRNAs; UPF1 binds RNA as part of its helicase function. action: ACCEPT reason: RNA binding is well supported (including HTP mRNA-interactome and direct assays) and is integral to UPF1 function. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0003723 - term: id: GO:0003724 label: RNA helicase activity evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: enables review: summary: RNA helicase activity is the core catalytic molecular function of UPF1, the central ATP-dependent helicase of nonsense-mediated mRNA decay. action: ACCEPT reason: Directly and repeatedly supported; UPF1 is an RNA-dependent helicase required for NMD. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: RNA-dependent helicase required for nonsense-mediated decay - term: id: GO:0004386 label: helicase activity evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: enables review: summary: General helicase activity, a parent of the specific RNA helicase activity that is core to UPF1. action: ACCEPT reason: Correct but less specific than RNA helicase activity; retained as a valid parent MF. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: RNA-dependent helicase required for nonsense-mediated decay - term: id: GO:0005524 label: ATP binding evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: enables review: summary: ATP binding, required for the ATPase/helicase cycle of UPF1. action: ACCEPT reason: ATP binding is integral to the SF1 helicase catalytic mechanism of UPF1. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: ATPase activity of UPF1 is - term: id: GO:0005634 label: nucleus evidence_type: IEA original_reference_id: GO_REF:0000044 qualifier: located_in review: summary: Nuclear localization; a minor UPF1 pool is nuclear (and UPF1 has nuclear/chromatin-associated roles). action: KEEP_AS_NON_CORE reason: Documented nuclear pool but the core NMD function is cytoplasmic; retained as non-core. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005634 - term: id: GO:0005737 label: cytoplasm evidence_type: IEA original_reference_id: GO_REF:0000120 qualifier: located_in review: summary: Cytoplasmic localization, a principal site of UPF1 action (NMD occurs on cytoplasmic translating mRNAs). action: ACCEPT reason: Well-supported cytoplasmic localization. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm' - term: id: GO:0008270 label: zinc ion binding evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: enables review: summary: Zinc ion binding (IEA); UPF1 has a CH (cysteine-histidine-rich) zinc-binding regulatory domain. action: ACCEPT reason: UPF1's N-terminal CH domain coordinates zinc and regulates helicase/UPF2 interaction. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0008270 - term: id: GO:0010468 label: regulation of gene expression evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in review: summary: Regulation of gene expression, a very general process annotation reflecting NMD-mediated control of transcript levels. action: KEEP_AS_NON_CORE reason: Too general to be a core annotation; subsumed by specific mRNA-decay terms. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0010468 - term: id: GO:0016787 label: hydrolase activity evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: enables review: summary: Generic hydrolase activity, an uninformative high-level parent of the ATPase/helicase activity. action: MARK_AS_OVER_ANNOTATED reason: Too general to be informative; the specific activities (RNA helicase, ATP hydrolysis) already capture this. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: ATPase activity of UPF1 is - term: id: GO:0016887 label: ATP hydrolysis activity evidence_type: IEA original_reference_id: GO_REF:0000116 qualifier: enables review: summary: ATP hydrolysis (ATPase) activity powers UPF1 translocation/unwinding and mRNP remodeling during NMD. action: ACCEPT reason: UPF1's ATPase activity is directly demonstrated and required for disassembly of mRNPs undergoing NMD. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: ATPase activity of UPF1 is - term: id: GO:0032574 label: 5'-3' RNA helicase activity evidence_type: IEA original_reference_id: GO_REF:0000003 qualifier: enables review: summary: UPF1 unwinds RNA with 5'-3' directionality; a precise statement of its helicase polarity. action: ACCEPT reason: Biochemically demonstrated 5'-3' RNA helicase activity; a useful specific child of RNA helicase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0032574 - term: id: GO:0043139 label: 5'-3' DNA helicase activity evidence_type: IEA original_reference_id: GO_REF:0000003 qualifier: enables review: summary: 5'-3' DNA helicase activity (IEA/IDA); UPF1 can unwind DNA in vitro. action: KEEP_AS_NON_CORE reason: In vitro DNA-unwinding activity; non-core relative to RNA helicase/NMD function. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: Also capable of unwinding double-stranded DNA and - term: id: GO:0048471 label: perinuclear region of cytoplasm evidence_type: IEA original_reference_id: GO_REF:0000044 qualifier: located_in review: summary: Perinuclear region of cytoplasm localization (by similarity). action: KEEP_AS_NON_CORE reason: Localization inferred by similarity; non-core. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0048471 - term: id: GO:1903313 label: positive regulation of mRNA metabolic process evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in review: summary: Positive regulation of mRNA metabolic process, a high-level process reflecting UPF1 control of mRNA turnover. action: KEEP_AS_NON_CORE reason: Correct but general; the specific NMD/mRNA-catabolism terms are more informative. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:1903313 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:11163187 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:12417715 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:12881429 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:15680326 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:16086026 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:16364915 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:16601204 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:17469741 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:18256688 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:18362360 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:18447585 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:19150429 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:19417104 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:19478851 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:19503078 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:19556969 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:21829167 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:23348841 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:23722113 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:24267889 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:33961781 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:35271311 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:40205054 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:16452507 qualifier: enables isoform: Q92900-2 review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:18066079 qualifier: enables isoform: Q92900-2 review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:18423202 qualifier: enables isoform: Q92900-2 review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:21145460 qualifier: enables isoform: Q92900-2 review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0032574 label: 5'-3' RNA helicase activity evidence_type: IDA original_reference_id: PMID:26138914 qualifier: enables review: summary: UPF1 unwinds RNA with 5'-3' directionality; a precise statement of its helicase polarity. action: ACCEPT reason: Biochemically demonstrated 5'-3' RNA helicase activity; a useful specific child of RNA helicase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0032574 - term: id: GO:0043139 label: 5'-3' DNA helicase activity evidence_type: IDA original_reference_id: PMID:26138914 qualifier: enables review: summary: 5'-3' DNA helicase activity (IEA/IDA); UPF1 can unwind DNA in vitro. action: KEEP_AS_NON_CORE reason: In vitro DNA-unwinding activity; non-core relative to RNA helicase/NMD function. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: Also capable of unwinding double-stranded DNA and - term: id: GO:0033678 label: 5'-3' DNA/RNA helicase activity evidence_type: IDA original_reference_id: PMID:18066079 qualifier: enables review: summary: 5'-3' DNA/RNA helicase activity; reflects UPF1's ability to translocate/unwind both nucleic acids. action: KEEP_AS_NON_CORE reason: Captures dual substrate unwinding; the RNA helicase activity is the core function. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: Also capable of unwinding double-stranded DNA and - term: id: GO:0033678 label: 5'-3' DNA/RNA helicase activity evidence_type: IDA original_reference_id: PMID:21419344 qualifier: enables review: summary: 5'-3' DNA/RNA helicase activity; reflects UPF1's ability to translocate/unwind both nucleic acids. action: KEEP_AS_NON_CORE reason: Captures dual substrate unwinding; the RNA helicase activity is the core function. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: Also capable of unwinding double-stranded DNA and - term: id: GO:0071025 label: RNA surveillance evidence_type: NAS original_reference_id: PMID:31131562 qualifier: involved_in review: summary: RNA surveillance, the broad quality-control category encompassing NMD in which UPF1 is the central factor. action: KEEP_AS_NON_CORE reason: Accurate high-level descriptor; the specific NMD term is the core annotation. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0071025 - term: id: GO:0170010 label: nonsense-mediated decay complex evidence_type: NAS original_reference_id: PMID:35640974 qualifier: part_of review: summary: UPF1 is a component of the nonsense-mediated decay (SURF/UPF1-UPF2-UPF3 surveillance) complex. action: ACCEPT reason: UPF1 is a defining subunit of the NMD effector complex. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0170010 - term: id: GO:2000623 label: negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay evidence_type: NAS original_reference_id: PMID:31131562 qualifier: involved_in review: summary: Negative regulation of NMD; reflects context-dependent modulation of NMD efficiency by UPF1/regulators. action: KEEP_AS_NON_CORE reason: NAS regulatory annotation; plausible in the context of NMD autoregulation but non-core relative to UPF1 driving NMD. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:2000623 - term: id: GO:2000624 label: positive regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay evidence_type: NAS original_reference_id: PMID:31131562 qualifier: involved_in review: summary: Positive regulation of NMD; UPF1 activity promotes NMD of target transcripts. action: KEEP_AS_NON_CORE reason: NAS regulatory annotation consistent with UPF1 function; non-core relative to the direct NMD term. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:2000624 - term: id: GO:0005654 label: nucleoplasm evidence_type: IDA original_reference_id: GO_REF:0000052 qualifier: located_in review: summary: Nucleoplasm localization (IDA), consistent with a nuclear UPF1 pool. action: KEEP_AS_NON_CORE reason: Minor nuclear localization; non-core relative to cytoplasmic NMD. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005654 - term: id: GO:0005829 label: cytosol evidence_type: IDA original_reference_id: GO_REF:0000052 qualifier: located_in review: summary: Cytosol, consistent with cytoplasmic NMD activity of UPF1. action: ACCEPT reason: Cytosolic localization agrees with the principal site of UPF1 function. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm' - term: id: GO:0005634 label: nucleus evidence_type: EXP original_reference_id: PMID:11163187 qualifier: located_in review: summary: Nuclear localization; a minor UPF1 pool is nuclear (and UPF1 has nuclear/chromatin-associated roles). action: KEEP_AS_NON_CORE reason: Documented nuclear pool but the core NMD function is cytoplasmic; retained as non-core. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005634 - term: id: GO:0005737 label: cytoplasm evidence_type: EXP original_reference_id: PMID:11163187 qualifier: located_in review: summary: Cytoplasmic localization, a principal site of UPF1 action (NMD occurs on cytoplasmic translating mRNAs). action: ACCEPT reason: Well-supported cytoplasmic localization. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm' - term: id: GO:0000184 label: nuclear-transcribed mRNA catabolic process, nonsense-mediated decay evidence_type: TAS original_reference_id: PMID:26255671 qualifier: involved_in review: summary: Nonsense-mediated mRNA decay of PTC-containing transcripts is the central biological process of UPF1; it is recruited at termination and triggers decay. action: ACCEPT reason: Core biological process; UPF1 is the central NMD factor. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: recruited to mRNAs upon translation termination - term: id: GO:0061158 label: 3'-UTR-mediated mRNA destabilization evidence_type: TAS original_reference_id: PMID:26255671 qualifier: involved_in review: summary: 3'-UTR-mediated mRNA destabilization; UPF1 (with UPF2/TDRD6) degrades mRNAs bearing long 3'UTRs, a recognized NMD-related branch. action: ACCEPT reason: Supported NMD-related destabilization function on long-3'UTR transcripts. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0061158 - term: id: GO:0071222 label: cellular response to lipopolysaccharide evidence_type: TAS original_reference_id: PMID:26255671 qualifier: involved_in review: summary: Cellular response to lipopolysaccharide (TAS); UPF1/NMD modulates inflammatory transcript stability. action: KEEP_AS_NON_CORE reason: Stimulus-response context for NMD targets; non-core. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0071222 - term: id: GO:0071347 label: cellular response to interleukin-1 evidence_type: TAS original_reference_id: PMID:26255671 qualifier: involved_in review: summary: Cellular response to interleukin-1 (TAS); NMD-related modulation of cytokine-response transcripts. action: KEEP_AS_NON_CORE reason: Stimulus-response context; non-core. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0071347 - term: id: GO:0016887 label: ATP hydrolysis activity evidence_type: IDA original_reference_id: PMID:30218034 qualifier: enables review: summary: ATP hydrolysis (ATPase) activity powers UPF1 translocation/unwinding and mRNP remodeling during NMD. action: ACCEPT reason: UPF1's ATPase activity is directly demonstrated and required for disassembly of mRNPs undergoing NMD. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: ATPase activity of UPF1 is - term: id: GO:0036121 label: double-stranded DNA helicase activity evidence_type: IDA original_reference_id: PMID:30218034 qualifier: enables review: summary: Double-stranded DNA helicase activity (IDA); UPF1 unwinds dsDNA in vitro. action: KEEP_AS_NON_CORE reason: In vitro dsDNA helicase activity; non-core. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: Also capable of unwinding double-stranded DNA and - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:26841701 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0044877 label: protein-containing complex binding evidence_type: IPI original_reference_id: PMID:26841701 qualifier: enables review: summary: Protein-containing complex binding; UPF1 engages multiprotein assemblies (EJC, SMG complexes). action: KEEP_AS_NON_CORE reason: Records complex association but is non-specific; the informative facts are the named complexes (EJC, SURF). supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0044877 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25220460 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:27974568 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0003723 label: RNA binding evidence_type: IDA original_reference_id: PMID:24726324 qualifier: enables review: summary: RNA binding underlies UPF1 recruitment to target mRNAs; UPF1 binds RNA as part of its helicase function. action: ACCEPT reason: RNA binding is well supported (including HTP mRNA-interactome and direct assays) and is integral to UPF1 function. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0003723 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:24726324 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0061014 label: positive regulation of mRNA catabolic process evidence_type: IDA original_reference_id: PMID:24726324 qualifier: involved_in review: summary: Positive regulation of mRNA catabolic process; UPF1 promotes decay of its target mRNAs. action: ACCEPT reason: Consistent with UPF1 driving mRNA degradation in NMD/SMD. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0061014 - term: id: GO:0061158 label: 3'-UTR-mediated mRNA destabilization evidence_type: IDA original_reference_id: PMID:24726324 qualifier: involved_in review: summary: 3'-UTR-mediated mRNA destabilization; UPF1 (with UPF2/TDRD6) degrades mRNAs bearing long 3'UTRs, a recognized NMD-related branch. action: ACCEPT reason: Supported NMD-related destabilization function on long-3'UTR transcripts. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0061158 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:19864460 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0032204 label: regulation of telomere maintenance evidence_type: IMP original_reference_id: PMID:17916692 qualifier: involved_in review: summary: Regulation of telomere maintenance (IMP); part of the UPF1 telomere function. action: KEEP_AS_NON_CORE reason: Moonlighting telomere role; non-core. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0032204 - term: id: GO:0044770 label: cell cycle phase transition evidence_type: IMP original_reference_id: PMID:16488880 qualifier: involved_in review: summary: Cell cycle phase transition (IMP); reflects UPF1 effects on cell-cycle progression (e.g. via histone mRNA decay / replication). action: KEEP_AS_NON_CORE reason: Downstream/indirect phenotype; non-core. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0044770 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:23788676 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25225333 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0061014 label: positive regulation of mRNA catabolic process evidence_type: IMP original_reference_id: PMID:25225333 qualifier: involved_in review: summary: Positive regulation of mRNA catabolic process; UPF1 promotes decay of its target mRNAs. action: ACCEPT reason: Consistent with UPF1 driving mRNA degradation in NMD/SMD. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0061014 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:26255671 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:24965446 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:26000482 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0000781 label: chromosome, telomeric region evidence_type: IDA original_reference_id: PMID:21829167 qualifier: located_in review: summary: Chromosome telomeric region localization, consistent with UPF1 telomere-associated role. action: KEEP_AS_NON_CORE reason: Telomere localization; moonlighting, non-core. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0000781 - term: id: GO:0032201 label: telomere maintenance via semi-conservative replication evidence_type: IDA original_reference_id: PMID:21829167 qualifier: involved_in review: summary: Telomere maintenance via semi-conservative replication; UPF1 supports telomere leading-strand synthesis. action: KEEP_AS_NON_CORE reason: Telomere-replication role (PMID:21829167); moonlighting, non-core. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0032201 - term: id: GO:0042162 label: telomeric repeat DNA binding evidence_type: IDA original_reference_id: PMID:17916692 qualifier: enables review: summary: Telomeric repeat DNA binding (IDA); part of a reported UPF1 role at telomeres with TPP1/telomerase. action: KEEP_AS_NON_CORE reason: Documented telomere-associated activity (PMID:21829167) but a moonlighting role distinct from core NMD. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: interacts with TPP1 and telomerase - term: id: GO:0042162 label: telomeric repeat DNA binding evidence_type: IDA original_reference_id: PMID:21829167 qualifier: enables review: summary: Telomeric repeat DNA binding (IDA); part of a reported UPF1 role at telomeres with TPP1/telomerase. action: KEEP_AS_NON_CORE reason: Documented telomere-associated activity (PMID:21829167) but a moonlighting role distinct from core NMD. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: interacts with TPP1 and telomerase - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-927813 qualifier: located_in review: summary: Cytosol, consistent with cytoplasmic NMD activity of UPF1. action: ACCEPT reason: Cytosolic localization agrees with the principal site of UPF1 function. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm' - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-927830 qualifier: located_in review: summary: Cytosol, consistent with cytoplasmic NMD activity of UPF1. action: ACCEPT reason: Cytosolic localization agrees with the principal site of UPF1 function. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm' - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-927836 qualifier: located_in review: summary: Cytosol, consistent with cytoplasmic NMD activity of UPF1. action: ACCEPT reason: Cytosolic localization agrees with the principal site of UPF1 function. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm' - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-927889 qualifier: located_in review: summary: Cytosol, consistent with cytoplasmic NMD activity of UPF1. action: ACCEPT reason: Cytosolic localization agrees with the principal site of UPF1 function. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm' - term: id: GO:0003723 label: RNA binding evidence_type: HDA original_reference_id: PMID:22658674 qualifier: enables review: summary: RNA binding underlies UPF1 recruitment to target mRNAs; UPF1 binds RNA as part of its helicase function. action: ACCEPT reason: RNA binding is well supported (including HTP mRNA-interactome and direct assays) and is integral to UPF1 function. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0003723 - term: id: GO:0003723 label: RNA binding evidence_type: HDA original_reference_id: PMID:22681889 qualifier: enables review: summary: RNA binding underlies UPF1 recruitment to target mRNAs; UPF1 binds RNA as part of its helicase function. action: ACCEPT reason: RNA binding is well supported (including HTP mRNA-interactome and direct assays) and is integral to UPF1 function. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0003723 - term: id: GO:0000956 label: nuclear-transcribed mRNA catabolic process evidence_type: IMP original_reference_id: PMID:23828042 qualifier: involved_in review: summary: Nuclear-transcribed mRNA catabolic process, a parent of the NMD process that UPF1 drives. action: KEEP_AS_NON_CORE reason: Correct but more general than the specific NMD term; retained as a valid parent process. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0000956 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:20930030 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-927789 qualifier: located_in review: summary: Cytosol, consistent with cytoplasmic NMD activity of UPF1. action: ACCEPT reason: Cytosolic localization agrees with the principal site of UPF1 function. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm' - term: id: GO:0005829 label: cytosol evidence_type: TAS original_reference_id: Reactome:R-HSA-927832 qualifier: located_in review: summary: Cytosol, consistent with cytoplasmic NMD activity of UPF1. action: ACCEPT reason: Cytosolic localization agrees with the principal site of UPF1 function. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm' - term: id: GO:0005634 label: nucleus evidence_type: IDA original_reference_id: PMID:18362360 qualifier: located_in review: summary: Nuclear localization; a minor UPF1 pool is nuclear (and UPF1 has nuclear/chromatin-associated roles). action: KEEP_AS_NON_CORE reason: Documented nuclear pool but the core NMD function is cytoplasmic; retained as non-core. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005634 - term: id: GO:0044530 label: supraspliceosomal complex evidence_type: IDA original_reference_id: PMID:18362360 qualifier: part_of review: summary: Supraspliceosomal complex; a nuclear assembly with which UPF1 was found associated. action: KEEP_AS_NON_CORE reason: Nuclear complex association; peripheral to the core cytoplasmic NMD function. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0044530 - term: id: GO:0003724 label: RNA helicase activity evidence_type: IDA original_reference_id: PMID:21419344 qualifier: enables review: summary: RNA helicase activity is the core catalytic molecular function of UPF1, the central ATP-dependent helicase of nonsense-mediated mRNA decay. action: ACCEPT reason: Directly and repeatedly supported; UPF1 is an RNA-dependent helicase required for NMD. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: RNA-dependent helicase required for nonsense-mediated decay - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:17932509 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0035145 label: exon-exon junction complex evidence_type: IDA original_reference_id: PMID:16601204 qualifier: part_of review: summary: Exon-exon junction complex; UPF1 functionally couples to the EJC during EJC-dependent NMD (via UPF2/UPF3). action: KEEP_AS_NON_CORE reason: UPF1 associates with the EJC-linked surveillance complex; recorded as a relevant but non-core part_of. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: exon junction complex - term: id: GO:0071044 label: histone mRNA catabolic process evidence_type: IMP original_reference_id: PMID:16086026 qualifier: involved_in review: summary: Histone mRNA catabolic process; UPF1 mediates replication-dependent histone mRNA degradation at the end of S phase, independent of UPF2. action: ACCEPT reason: Well-documented UPF1 function in histone mRNA decay; a genuine UPF1-dependent decay pathway. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: replication-dependent histone mRNA degradation - term: id: GO:0000184 label: nuclear-transcribed mRNA catabolic process, nonsense-mediated decay evidence_type: IMP original_reference_id: PMID:18369367 qualifier: involved_in review: summary: Nonsense-mediated mRNA decay of PTC-containing transcripts is the central biological process of UPF1; it is recruited at termination and triggers decay. action: ACCEPT reason: Core biological process; UPF1 is the central NMD factor. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: recruited to mRNAs upon translation termination - term: id: GO:0000184 label: nuclear-transcribed mRNA catabolic process, nonsense-mediated decay evidence_type: IDA original_reference_id: PMID:17468741 qualifier: involved_in review: summary: Nonsense-mediated mRNA decay of PTC-containing transcripts is the central biological process of UPF1; it is recruited at termination and triggers decay. action: ACCEPT reason: Core biological process; UPF1 is the central NMD factor. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: recruited to mRNAs upon translation termination - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:17468741 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0000184 label: nuclear-transcribed mRNA catabolic process, nonsense-mediated decay evidence_type: TAS original_reference_id: PMID:8855285 qualifier: involved_in review: summary: Nonsense-mediated mRNA decay of PTC-containing transcripts is the central biological process of UPF1; it is recruited at termination and triggers decay. action: ACCEPT reason: Core biological process; UPF1 is the central NMD factor. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: recruited to mRNAs upon translation termination - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:11544179 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0000785 label: chromatin evidence_type: IDA original_reference_id: PMID:16488880 qualifier: located_in review: summary: Chromatin localization (IDA) associated with UPF1 nuclear/replication roles. action: KEEP_AS_NON_CORE reason: Nuclear chromatin association; non-core relative to cytoplasmic NMD. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0000785 - term: id: GO:0003682 label: chromatin binding evidence_type: IDA original_reference_id: PMID:16488880 qualifier: enables review: summary: Chromatin binding (IDA), consistent with the chromatin-associated UPF1 pool. action: KEEP_AS_NON_CORE reason: Chromatin binding; moonlighting/non-core. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0003682 - term: id: GO:0003724 label: RNA helicase activity evidence_type: IDA original_reference_id: PMID:12554878 qualifier: enables review: summary: RNA helicase activity is the core catalytic molecular function of UPF1, the central ATP-dependent helicase of nonsense-mediated mRNA decay. action: ACCEPT reason: Directly and repeatedly supported; UPF1 is an RNA-dependent helicase required for NMD. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: RNA-dependent helicase required for nonsense-mediated decay - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:14636577 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:16488880 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0006260 label: DNA replication evidence_type: IMP original_reference_id: PMID:16488880 qualifier: involved_in review: summary: DNA replication (IMP); UPF1 has a reported role in S-phase/replication. action: KEEP_AS_NON_CORE reason: Reported replication role (also linked to histone mRNA decay timing); non-core relative to NMD. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0006260 - term: id: GO:0006281 label: DNA repair evidence_type: IDA original_reference_id: PMID:16488880 qualifier: involved_in review: summary: DNA repair (IDA); reported chromatin/replication-associated UPF1 activity. action: KEEP_AS_NON_CORE reason: Peripheral genome-maintenance role; non-core. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0006281 - term: id: GO:0006406 label: mRNA export from nucleus evidence_type: TAS original_reference_id: PMID:16488880 qualifier: involved_in review: summary: mRNA export from nucleus (TAS); a reported ancillary role in mRNA metabolism. action: KEEP_AS_NON_CORE reason: Peripheral mRNA-metabolism role; non-core relative to NMD. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0006406 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:9620853 qualifier: enables review: summary: Interaction evidence captured under the uninformative generic protein binding term. UPF1 is a hub with many physical partners; this records a real interaction but does not by itself specify a function. action: KEEP_AS_NON_CORE reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; kept as non-core because it records a genuine interaction but the core molecular function is the RNA helicase/ATPase activity. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0005515 - term: id: GO:0006449 label: regulation of translational termination evidence_type: IMP original_reference_id: PMID:9620853 qualifier: involved_in review: summary: Regulation of translational termination; UPF1 acts at terminating ribosomes (SURF complex with eRF1/eRF3) to couple termination to decay. action: ACCEPT reason: Supported by UPF1 association with release factors at termination as the trigger for NMD. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: recruited to mRNAs upon translation termination - term: id: GO:0000184 label: nuclear-transcribed mRNA catabolic process, nonsense-mediated decay evidence_type: NAS original_reference_id: PMID:10999600 qualifier: involved_in review: summary: Nonsense-mediated mRNA decay of PTC-containing transcripts is the central biological process of UPF1; it is recruited at termination and triggers decay. action: ACCEPT reason: Core biological process; UPF1 is the central NMD factor. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: recruited to mRNAs upon translation termination - term: id: GO:0003723 label: RNA binding evidence_type: NAS original_reference_id: PMID:10999600 qualifier: enables review: summary: RNA binding underlies UPF1 recruitment to target mRNAs; UPF1 binds RNA as part of its helicase function. action: ACCEPT reason: RNA binding is well supported (including HTP mRNA-interactome and direct assays) and is integral to UPF1 function. supported_by: - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0003723 - term: id: GO:0006449 label: regulation of translational termination evidence_type: NAS original_reference_id: PMID:10999600 qualifier: involved_in review: summary: Regulation of translational termination; UPF1 acts at terminating ribosomes (SURF complex with eRF1/eRF3) to couple termination to decay. action: ACCEPT reason: Supported by UPF1 association with release factors at termination as the trigger for NMD. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: recruited to mRNAs upon translation termination - term: id: GO:0004386 label: helicase activity evidence_type: NAS original_reference_id: PMID:9064659 qualifier: enables review: summary: General helicase activity, a parent of the specific RNA helicase activity that is core to UPF1. action: ACCEPT reason: Correct but less specific than RNA helicase activity; retained as a valid parent MF. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: RNA-dependent helicase required for nonsense-mediated decay - term: id: GO:0005737 label: cytoplasm evidence_type: NAS original_reference_id: PMID:9064659 qualifier: located_in review: summary: Cytoplasmic localization, a principal site of UPF1 action (NMD occurs on cytoplasmic translating mRNAs). action: ACCEPT reason: Well-supported cytoplasmic localization. supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: 'SUBCELLULAR LOCATION: Cytoplasm' core_functions: - description: ATP-dependent RNA helicase / ATPase that, recruited at translation termination, remodels and disassembles target mRNPs to drive nonsense-mediated mRNA decay. molecular_function: id: GO:0003724 label: RNA helicase activity locations: - id: GO:0005829 label: cytosol supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: RNA-dependent helicase required for nonsense-mediated decay - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: ATPase activity of UPF1 is - description: Central effector of nonsense-mediated mRNA decay of premature-termination-codon-containing transcripts; recruited at terminating ribosomes and, via SMG1 phosphorylation and the UPF2/UPF3-EJC surveillance complex, commits target mRNAs to degradation. molecular_function: id: GO:0003724 label: RNA helicase activity locations: - id: GO:0005829 label: cytosol supported_by: - reference_id: file:human/UPF1/UPF1-uniprot.txt supporting_text: recruited to mRNAs upon translation termination - reference_id: file:human/UPF1/UPF1-goa.tsv supporting_text: GO:0000184 proposed_new_terms: [] suggested_questions: - question: Which of UPF1’s non-NMD roles (telomere replication, DNA repair, histone mRNA decay) reflect distinct molecular activities versus indirect consequences of perturbing NMD? - question: How does the phosphorylation/dephosphorylation cycle of UPF1 (SMG1 vs PP2A) quantitatively gate the commitment of a target mRNA to decay? suggested_experiments: - description: Separation-of-function UPF1 mutants (helicase-dead vs phospho-site vs CH-domain) followed by transcriptome-wide NMD-target profiling to dissect catalytic from scaffolding roles. - description: Single-molecule assays of UPF1 ATPase-driven translocation/mRNP disassembly on reconstituted EJC-containing substrates to quantify the remodeling step that commits mRNAs to decay. references: - id: GO_REF:0000002 title: Gene Ontology annotation through association of InterPro records with GO terms findings: [] - id: GO_REF:0000003 title: Gene Ontology annotation based on Enzyme Commission mapping findings: [] - id: GO_REF:0000033 title: Annotation inferences using phylogenetic trees findings: [] - id: GO_REF:0000044 title: Gene Ontology annotation through association of UniProtKB keywords with GO terms findings: [] - id: GO_REF:0000052 title: Gene Ontology annotation based on curation of immunofluorescence data findings: [] - id: GO_REF:0000116 title: Gene Ontology annotation based on the InterPro2GO/ARBA mapping findings: [] - id: GO_REF:0000117 title: Electronic Gene Ontology annotations created by ARBA machine learning models findings: [] - id: GO_REF:0000120 title: Combined Automated Annotation using Multiple IEA Methods findings: [] - id: PMID:10999600 title: Characterization of the biochemical properties of the human Upf1 gene product that is involved in nonsense-mediated mRNA decay. findings: [] reference_review: relevance: HIGH correctness: VERIFIED review_notes: "Cached PMID_10999600 title matches; biochemical characterization of human UPF1's RNA-dependent ATPase/helicase activity, supporting the GO:0003724 helicase core function (consistent with GOA helicase/NMD rows)." - id: PMID:11163187 title: Human Upf proteins target an mRNA for nonsense-mediated decay when bound downstream of a termination codon. findings: [] - id: PMID:11544179 title: Human SMG-1, a novel phosphatidylinositol 3-kinase-related protein kinase, associates with components of the mRNA surveillance complex and is involved in the regulation of nonsense-mediated mRNA decay. findings: [] - id: PMID:12417715 title: Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay. findings: [] - id: PMID:12554878 title: 'Characterization of human Smg5/7a: a protein with similarities to Caenorhabditis elegans SMG5 and SMG7 that functions in the dephosphorylation of Upf1.' findings: [] - id: PMID:12881429 title: Interaction between Ski7p and Upf1p is required for nonsense-mediated 3'-to-5' mRNA decay in yeast. findings: [] - id: PMID:14636577 title: Phosphorylation of hUPF1 induces formation of mRNA surveillance complexes containing hSMG-5 and hSMG-7. findings: [] - id: PMID:15680326 title: Mammalian Staufen1 recruits Upf1 to specific mRNA 3'UTRs so as to elicit mRNA decay. findings: [] - id: PMID:16086026 title: Regulated degradation of replication-dependent histone mRNAs requires both ATR and Upf1. findings: [] - id: PMID:16364915 title: Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping. findings: [] - id: PMID:16452507 title: Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upf1 phosphorylation and nonsense-mediated mRNA decay. findings: [] - id: PMID:16488880 title: The human RNA surveillance factor UPF1 is required for S phase progression and genome stability. findings: [] - id: PMID:16601204 title: Functions of hUpf3a and hUpf3b in nonsense-mediated mRNA decay and translation. findings: [] - id: PMID:17468741 title: Human INT6/eIF3e is required for nonsense-mediated mRNA decay. findings: [] - id: PMID:17469741 title: Results of the determination of serum markers in patients with malignant melanoma. findings: [] reference_review: relevance: NONE correctness: WRONG_IDENTIFIER review_notes: Cited as a UPF1 protein binding (IPI) source but the resolved title is a melanoma serum-marker study unrelated to UPF1; appears to be an incorrect identifier in the GOA/IntAct record. - id: PMID:17916692 title: Telomeric repeat containing RNA and RNA surveillance factors at mammalian chromosome ends. findings: [] - id: PMID:17932509 title: Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells. findings: [] - id: PMID:18066079 title: NMD factors UPF2 and UPF3 bridge UPF1 to the exon junction complex and stimulate its RNA helicase activity. findings: [] - id: PMID:18256688 title: Interactions between UPF1, eRFs, PABP and the exon junction complex suggest an integrated model for mammalian NMD pathways. findings: [] - id: PMID:18362360 title: The editing enzyme ADAR1 and the mRNA surveillance protein hUpf1 interact in the cell nucleus. findings: [] - id: PMID:18369367 title: NMD resulting from encephalomyocarditis virus IRES-directed translation initiation seems to be restricted to CBP80/20-bound mRNA. findings: [] - id: PMID:18423202 title: Upf1 phosphorylation triggers translational repression during nonsense-mediated mRNA decay. findings: [] - id: PMID:18447585 title: A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. findings: [] - id: PMID:19150429 title: Human proline-rich nuclear receptor coregulatory protein 2 mediates an interaction between mRNA surveillance machinery and decapping complex. findings: [] - id: PMID:19417104 title: SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay. findings: [] - id: PMID:19478851 title: The hierarchy of exon-junction complex assembly by the spliceosome explains key features of mammalian nonsense-mediated mRNA decay. findings: [] - id: PMID:19503078 title: A UPF3-mediated regulatory switch that maintains RNA surveillance. findings: [] - id: PMID:19556969 title: Unusual bipartite mode of interaction between the nonsense-mediated decay factors, UPF1 and UPF2. findings: [] - id: PMID:19864460 title: Mammalian pre-mRNA 3' end processing factor CF I m 68 functions in mRNA export. findings: [] - id: PMID:20930030 title: SMG6 interacts with the exon junction complex via two conserved EJC-binding motifs (EBMs) required for nonsense-mediated mRNA decay. findings: [] - id: PMID:21145460 title: Upf1 ATPase-dependent mRNP disassembly is required for completion of nonsense- mediated mRNA decay. findings: [] reference_review: relevance: HIGH correctness: VERIFIED review_notes: "Cached PMID_21145460 title matches; demonstrates UPF1 ATPase-driven mRNP disassembly is required to complete NMD, directly supporting the helicase/ATPase effector core function." - id: PMID:21419344 title: Molecular mechanisms for the RNA-dependent ATPase activity of Upf1 and its regulation by Upf2. findings: [] - id: PMID:21829167 title: Human UPF1 interacts with TPP1 and telomerase and sustains telomere leading-strand replication. findings: [] - id: PMID:22658674 title: Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. findings: [] - id: PMID:22681889 title: The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. findings: [] - id: PMID:23348841 title: An unusual arrangement of two 14-3-3-like domains in the SMG5-SMG7 heterodimer is required for efficient nonsense-mediated mRNA decay. findings: [] - id: PMID:23722113 title: Non-structural protein 1 of influenza viruses inhibits rapid mRNA degradation mediated by double-stranded RNA-binding protein, staufen1. findings: [] - id: PMID:23788676 title: The RNA helicase Ddx5/p68 binds to hUpf3 and enhances NMD of Ddx17/p72 and Smg5 mRNA. findings: [] - id: PMID:23828042 title: DHX34 and NBAS form part of an autoregulatory NMD circuit that regulates endogenous RNA targets in human cells, zebrafish and Caenorhabditis elegans. findings: [] - id: PMID:24267889 title: Affinity proteomics reveals human host factors implicated in discrete stages of LINE-1 retrotransposition. findings: [] - id: PMID:24726324 title: MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs. findings: [] - id: PMID:24965446 title: Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex. findings: [] - id: PMID:25220460 title: The RNA helicase DHX34 activates NMD by promoting a transition from the surveillance to the decay-inducing complex. findings: [] - id: PMID:25225333 title: Plakophilins 1 and 3 bind to FXR1 and thereby influence the mRNA stability of desmosomal proteins. findings: [] - id: PMID:26000482 title: Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms. findings: [] - id: PMID:26138914 title: Human Upf1 is a highly processive RNA helicase and translocase with RNP remodelling activities. findings: [] reference_review: relevance: HIGH correctness: VERIFIED review_notes: "Cached PMID_26138914 title matches; characterizes human UPF1 as a processive RNA helicase/translocase with RNP-remodeling activity, supporting GO:0003724." - id: PMID:26255671 title: Regnase-1 and Roquin regulate inflammatory mRNAs. findings: [] - id: PMID:26841701 title: The RNA helicase DHX34 functions as a scaffold for SMG1-mediated UPF1 phosphorylation. findings: [] - id: PMID:27974568 title: IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10. findings: [] - id: PMID:30218034 title: UPF1-like helicase grip on nucleic acids dictates processivity. findings: [] - id: PMID:31131562 title: 'Nonsense-mediated mRNA decay: The challenge of telling right from wrong in a complex transcriptome.' findings: [] - id: PMID:33961781 title: Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. findings: [] - id: PMID:35271311 title: 'OpenCell: Endogenous tagging for the cartography of human cellular organization.' findings: [] - id: PMID:35640974 title: Structures of nonsense-mediated mRNA decay factors UPF3B and UPF3A in complex with UPF2 reveal molecular basis for competitive binding and for neurodevelopmental disorder-causing mutation. findings: [] - id: PMID:40205054 title: Multimodal cell maps as a foundation for structural and functional genomics. findings: [] - id: PMID:8855285 title: Mammalian orthologues of a yeast regulator of nonsense transcript stability. findings: [] - id: PMID:9064659 title: Cloning and characterization of HUPF1, a human homolog of the Saccharomyces cerevisiae nonsense mRNA-reducing UPF1 protein. findings: [] - id: PMID:9620853 title: The surveillance complex interacts with the translation release factors to enhance termination and degrade aberrant mRNAs. findings: [] - id: Reactome:R-HSA-927789 title: 'Reactome: Formation of the SURF complex (cytosol)' findings: [] - id: Reactome:R-HSA-927813 title: 'Reactome: nonsense-mediated decay (cytosol)' findings: [] - id: Reactome:R-HSA-927830 title: 'Reactome: nonsense-mediated decay (cytosol)' findings: [] - id: Reactome:R-HSA-927832 title: 'Reactome: nonsense-mediated decay (cytosol)' findings: [] - id: Reactome:R-HSA-927836 title: 'Reactome: nonsense-mediated decay (cytosol)' findings: [] - id: Reactome:R-HSA-927889 title: 'Reactome: nonsense-mediated decay (cytosol)' findings: []