UPF2 (regulator of nonsense transcripts 2; RENT2) is a core adaptor of the nonsense-mediated mRNA decay (NMD) pathway. Built around tandem MIF4G domains with a C-terminal UPF1-binding region, UPF2 bridges UPF1 (bound to the release factors eRF1/eRF3 at a terminating ribosome) to UPF3B and the exon-junction complex, nucleating the UPF1-UPF2-UPF3 surveillance complex that activates NMD. Binding of UPF2 (together with UPF3B) relieves UPF1 autoinhibition and stimulates its ATPase and RNA helicase activities, committing premature-termination-codon and certain long-3'UTR transcripts to decay. UPF2 acts mainly at the cytoplasmic/perinuclear face of the nuclear envelope and in the cytoplasm, binds spliced mRNA, and has additional reported associations (e.g. telomere/TERRA, mRNA export) peripheral to its core NMD adaptor role.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0035145 exon-exon junction complex | IBA GO_REF:0000033 | ACCEPT | Summary: UPF2 is functionally associated with the exon-junction complex; it is recruited by EJC-bound UPF3B and bridges the EJC to UPF1 during NMD. Reason: Central to UPF2 function as the EJC-UPF1 bridging adaptor in EJC-dependent NMD. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt Recruited by UPF3B associated with the EJC core |
| GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay | IEA GO_REF:0000002 | ACCEPT | Summary: Nonsense-mediated mRNA decay is the core biological process of UPF2; it nucleates the UPF1-UPF2-UPF3 surveillance complex that activates NMD. Reason: Core NMD process; UPF2 is an essential NMD factor. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt Involved in nonsense-mediated decay (NMD) |
| GO:0003723 RNA binding | IEA GO_REF:0000002 | ACCEPT | Summary: UPF2 binds spliced mRNA, consistent with its association with mRNP/EJC during NMD. Reason: RNA (spliced mRNA) binding is part of UPF2 engagement with target mRNPs. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt Binds spliced mRNA |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | ACCEPT | Summary: Cytoplasmic localization, where NMD occurs; UPF2 acts at the cytoplasmic face of the nuclear envelope and in the cytoplasm. Reason: Well-supported cytoplasmic/perinuclear localization. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, perinuclear region |
| GO:0048471 perinuclear region of cytoplasm | IEA GO_REF:0000044 | ACCEPT | Summary: Perinuclear region of cytoplasm; UPF2 concentrates at the cytoplasmic side of the nuclear envelope where it meets EJC-bound mRNPs. Reason: Directly documented perinuclear localization for UPF2. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, perinuclear region |
| GO:0005515 protein binding | IPI PMID:11163187 Human Upf proteins target an mRNA for nonsense-mediated deca... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:12417715 Identification of a human decapping complex associated with ... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:15231747 A protein interaction framework for human mRNA degradation. | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:15680326 Mammalian Staufen1 recruits Upf1 to specific mRNA 3'UTRs so ... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:16086026 Regulated degradation of replication-dependent histone mRNAs... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:16452507 Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to th... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:16601204 Functions of hUpf3a and hUpf3b in nonsense-mediated mRNA dec... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:17469741 Results of the determination of serum markers in patients wi... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:18066079 NMD factors UPF2 and UPF3 bridge UPF1 to the exon junction c... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:18256688 Interactions between UPF1, eRFs, PABP and the exon junction ... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:19150429 Human proline-rich nuclear receptor coregulatory protein 2 m... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:19417104 SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, re... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:19478851 The hierarchy of exon-junction complex assembly by the splic... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:19503078 A UPF3-mediated regulatory switch that maintains RNA surveil... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:19556969 Unusual bipartite mode of interaction between the nonsense-m... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:20371770 AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:20479275 Insights into the recruitment of the NMD machinery from the ... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:21145460 Upf1 ATPase-dependent mRNP disassembly is required for compl... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:22522823 The cryo-EM structure of the UPF-EJC complex shows UPF1 pois... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:26496610 A human interactome in three quantitative dimensions organiz... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0071025 RNA surveillance | NAS PMID:31131562 Nonsense-mediated mRNA decay: The challenge of telling right... | KEEP AS NON CORE | Summary: RNA surveillance, the broad quality-control category encompassing NMD in which UPF2 is an essential adaptor. Reason: Accurate high-level descriptor; the specific NMD term is the core annotation. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0071025 |
| GO:0170010 nonsense-mediated decay complex | NAS PMID:35640974 Structures of nonsense-mediated mRNA decay factors UPF3B and... | ACCEPT | Summary: UPF2 is a component of the nonsense-mediated decay (UPF1-UPF2-UPF3) surveillance complex. Reason: UPF2 is a defining subunit of the NMD effector complex. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0170010 |
| GO:2000623 negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay | NAS PMID:31131562 Nonsense-mediated mRNA decay: The challenge of telling right... | KEEP AS NON CORE | Summary: Negative regulation of NMD; context-dependent modulation of NMD efficiency. Reason: NAS regulatory annotation; non-core relative to UPF2 driving NMD. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:2000623 |
| GO:2000624 positive regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay | NAS PMID:31131562 Nonsense-mediated mRNA decay: The challenge of telling right... | KEEP AS NON CORE | Summary: Positive regulation of NMD; UPF2 promotes NMD by stimulating UPF1 ATPase/helicase activity. Reason: NAS regulatory annotation consistent with UPF2 function; non-core relative to the direct NMD term. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:2000624 |
| GO:0005829 cytosol | IDA GO_REF:0000052 | ACCEPT | Summary: Cytosol localization, consistent with cytoplasmic NMD activity of UPF2. Reason: Cytosolic localization agrees with the principal site of UPF2 function. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, perinuclear region |
| GO:0005737 cytoplasm | ISS GO_REF:0000024 | ACCEPT | Summary: Cytoplasmic localization, where NMD occurs; UPF2 acts at the cytoplasmic face of the nuclear envelope and in the cytoplasm. Reason: Well-supported cytoplasmic/perinuclear localization. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, perinuclear region |
| GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay | IMP PMID:19556969 Unusual bipartite mode of interaction between the nonsense-m... | ACCEPT | Summary: Nonsense-mediated mRNA decay is the core biological process of UPF2; it nucleates the UPF1-UPF2-UPF3 surveillance complex that activates NMD. Reason: Core NMD process; UPF2 is an essential NMD factor. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt Involved in nonsense-mediated decay (NMD) |
| GO:0005515 protein binding | IPI PMID:25220460 The RNA helicase DHX34 activates NMD by promoting a transiti... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:23788676 The RNA helicase Ddx5/p68 binds to hUpf3 and enhances NMD of... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005634 nucleus | IDA PMID:21829167 Human UPF1 interacts with TPP1 and telomerase and sustains t... | KEEP AS NON CORE | Summary: Nuclear localization (IDA); a minor UPF2 pool / shuttling consistent with EJC association near the nuclear envelope. Reason: Minor nuclear pool; the core adaptor function acts at the cytoplasmic/perinuclear face. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005634 |
| GO:0042162 telomeric repeat DNA binding | IDA PMID:17916692 Telomeric repeat containing RNA and RNA surveillance factors... | KEEP AS NON CORE | Summary: Telomeric repeat DNA binding (IDA); part of a reported NMD-factor association with telomeres/TERRA. Reason: Telomere-associated activity (PMID:17916692) is a moonlighting role distinct from core NMD adaptor function. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0042162 |
| GO:0005515 protein binding | IPI PMID:20930030 SMG6 interacts with the exon junction complex via two conser... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005829 cytosol | TAS Reactome:R-HSA-927813 | ACCEPT | Summary: Cytosol localization, consistent with cytoplasmic NMD activity of UPF2. Reason: Cytosolic localization agrees with the principal site of UPF2 function. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, perinuclear region |
| GO:0005829 cytosol | TAS Reactome:R-HSA-927832 | ACCEPT | Summary: Cytosol localization, consistent with cytoplasmic NMD activity of UPF2. Reason: Cytosolic localization agrees with the principal site of UPF2 function. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, perinuclear region |
| GO:0005829 cytosol | TAS Reactome:R-HSA-927836 | ACCEPT | Summary: Cytosol localization, consistent with cytoplasmic NMD activity of UPF2. Reason: Cytosolic localization agrees with the principal site of UPF2 function. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, perinuclear region |
| GO:0005829 cytosol | TAS Reactome:R-HSA-927889 | ACCEPT | Summary: Cytosol localization, consistent with cytoplasmic NMD activity of UPF2. Reason: Cytosolic localization agrees with the principal site of UPF2 function. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, perinuclear region |
| GO:0005515 protein binding | IPI PMID:17468741 Human INT6/eIF3e is required for nonsense-mediated mRNA deca... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0035145 exon-exon junction complex | IDA PMID:16601204 Functions of hUpf3a and hUpf3b in nonsense-mediated mRNA dec... | ACCEPT | Summary: UPF2 is functionally associated with the exon-junction complex; it is recruited by EJC-bound UPF3B and bridges the EJC to UPF1 during NMD. Reason: Central to UPF2 function as the EJC-UPF1 bridging adaptor in EJC-dependent NMD. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt Recruited by UPF3B associated with the EJC core |
| GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay | IMP PMID:18369367 NMD resulting from encephalomyocarditis virus IRES-directed ... | ACCEPT | Summary: Nonsense-mediated mRNA decay is the core biological process of UPF2; it nucleates the UPF1-UPF2-UPF3 surveillance complex that activates NMD. Reason: Core NMD process; UPF2 is an essential NMD factor. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt Involved in nonsense-mediated decay (NMD) |
| GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay | TAS PMID:17468741 Human INT6/eIF3e is required for nonsense-mediated mRNA deca... | ACCEPT | Summary: Nonsense-mediated mRNA decay is the core biological process of UPF2; it nucleates the UPF1-UPF2-UPF3 surveillance complex that activates NMD. Reason: Core NMD process; UPF2 is an essential NMD factor. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt Involved in nonsense-mediated decay (NMD) |
| GO:0005515 protein binding | IPI PMID:11544179 Human SMG-1, a novel phosphatidylinositol 3-kinase-related p... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay | TAS PMID:16488880 The human RNA surveillance factor UPF1 is required for S pha... | ACCEPT | Summary: Nonsense-mediated mRNA decay is the core biological process of UPF2; it nucleates the UPF1-UPF2-UPF3 surveillance complex that activates NMD. Reason: Core NMD process; UPF2 is an essential NMD factor. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt Involved in nonsense-mediated decay (NMD) |
| GO:0005515 protein binding | IPI PMID:14636577 Phosphorylation of hUPF1 induces formation of mRNA surveilla... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005515 protein binding | IPI PMID:16488880 The human RNA surveillance factor UPF1 is required for S pha... | KEEP AS NON CORE | Summary: Interaction captured under the uninformative generic protein binding term. UPF2 is a central NMD adaptor with many partners (UPF1, UPF3A/B, EJC, SMG factors); this records a real interaction but does not by itself specify function. Reason: Bare protein binding (GO:0005515) is uninformative per curation guidelines; retained as non-core because the informative function is its adaptor role bridging UPF1 to UPF3-EJC. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005515 |
| GO:0005634 nucleus | IDA PMID:14636577 Phosphorylation of hUPF1 induces formation of mRNA surveilla... | KEEP AS NON CORE | Summary: Nuclear localization (IDA); a minor UPF2 pool / shuttling consistent with EJC association near the nuclear envelope. Reason: Minor nuclear pool; the core adaptor function acts at the cytoplasmic/perinuclear face. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0005634 |
| GO:0005737 cytoplasm | IDA PMID:14636577 Phosphorylation of hUPF1 induces formation of mRNA surveilla... | ACCEPT | Summary: Cytoplasmic localization, where NMD occurs; UPF2 acts at the cytoplasmic face of the nuclear envelope and in the cytoplasm. Reason: Well-supported cytoplasmic/perinuclear localization. Supporting Evidence: file:human/UPF2/UPF2-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm, perinuclear region |
| GO:0006406 mRNA export from nucleus | TAS PMID:16488880 The human RNA surveillance factor UPF1 is required for S pha... | KEEP AS NON CORE | Summary: mRNA export from nucleus (TAS); a reported ancillary role linked to UPF2/NMD-factor mRNP metabolism. Reason: Peripheral mRNA-metabolism role; non-core relative to NMD adaptor function. Supporting Evidence: file:human/UPF2/UPF2-goa.tsv GO:0006406 |
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Download this section (compressed HTML)Q: How is the competitive binding of UPF3A versus UPF3B to UPF2 regulated, and how does it tune NMD efficiency on different transcripts?
Q: Which UPF2-dependent NMD branches require the EJC versus operating in EJC-independent NMD?
Experiment: Domain-resolved UPF2 mutants (MIF4G vs UPF1-binding vs UPF3-binding) with transcriptome-wide NMD-target profiling to separate bridging from UPF1-activation functions.
Experiment: In vitro reconstitution measuring UPF2/UPF3B stimulation of UPF1 ATPase/helicase activity on EJC-containing substrates to quantify the activation step.
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