VPS37B is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and MVB12/UBAP-family subunits. It supports ESCRT-I-dependent endosomal sorting of ubiquitinated cargo into multivesicular bodies and contributes to ESCRT-I membrane-remodeling contexts. Current local evidence does not support transferring the VPS37A-specific phagophore-closure role to VPS37B as a core annotation.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | IBA GO_REF:0000033 | ACCEPT | Summary: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway is supported as part of the core VPS37B/ESCRT-I endosomal cargo-sorting role. Reason: VPS37B/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting and MVB biogenesis. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0000813 ESCRT I complex | IBA GO_REF:0000033 | ACCEPT | Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A |
| GO:0006612 protein targeting to membrane | IBA GO_REF:0000033 | MODIFY | Summary: protein targeting to membrane is too broad for VPS37B ESCRT-I function. Reason: The supported process is ESCRT-I-dependent endosomal cargo sorting into the MVB pathway, not generic protein targeting. Proposed replacements: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway multivesicular body assembly Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0006623 protein targeting to vacuole | IBA GO_REF:0000033 | MODIFY | Summary: protein targeting to vacuole is too broad for VPS37B ESCRT-I function. Reason: The supported process is ESCRT-I-dependent endosomal cargo sorting into the MVB pathway, not generic protein targeting. Proposed replacements: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway multivesicular body assembly Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0000813 ESCRT I complex | IEA GO_REF:0000117 | ACCEPT | Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A |
| GO:0016236 macroautophagy | IEA GO_REF:0000117 | MARK AS OVER ANNOTATED | Summary: Macroautophagy is over-annotated for VPS37B. Reason: The accessible ESCRT autophagy evidence is broad or VPS37A-specific; there is not enough direct evidence to make VPS37B a phagophore-closure/autophagosome assembly factor. Proposed replacements: ESCRT I complex membrane fission Supporting Evidence: PMID:20588296 viral budding, cytokinesis and, probably, autophagy PMID:31519728 the ESCRT-I subunit VPS37A as a critical component for phagophore closure PMID:31519728 required for autophagosome completion but dispensable for ESCRT-I complex formation |
| GO:0031902 late endosome membrane | IEA GO_REF:0000044 | ACCEPT | Summary: late endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments |
| GO:0036258 multivesicular body assembly | IEA GO_REF:0000117 | ACCEPT | Summary: multivesicular body assembly is supported as part of the core VPS37B/ESCRT-I endosomal cargo-sorting role. Reason: VPS37B/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting and MVB biogenesis. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0039702 viral budding via host ESCRT complex | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: viral budding via host ESCRT complex is supported as a secondary context but is not the core VPS37B proteostasis function. Reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy |
| GO:0048306 calcium-dependent protein binding | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Calcium-dependent protein binding is supported but secondary. Reason: VPS37B participates in an ALG-2/ALIX/ESCRT-I calcium-dependent ternary complex, but this is not the core VPS37B proteostasis function. Supporting Evidence: PMID:23924735 VPS37B and VPS37C appeared to interact with ALG-2 PMID:23924735 adaptor protein that bridges ALIX and ESCRT-I |
| GO:0005515 protein binding | IPI PMID:21516116 Next-generation sequencing to generate interactome datasets. | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0005515 protein binding | IPI PMID:25416956 A proteome-scale map of the human interactome network. | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0005515 protein binding | IPI PMID:26871637 Widespread Expansion of Protein Interaction Capabilities by ... | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0005515 protein binding | IPI PMID:28514442 Architecture of the human interactome defines protein commun... | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0005515 protein binding | IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari... | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0005515 protein binding | IPI PMID:32296183 A reference map of the human binary protein interactome. | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0005515 protein binding | IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling... | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0005515 protein binding | IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce... | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0000813 ESCRT I complex | IPI PMID:18005716 Identification of human MVB12 proteins as ESCRT-I subunits t... | ACCEPT | Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A |
| GO:0000813 ESCRT I complex | IPI PMID:21757351 UBAP1 is a component of an endosome-specific ESCRT-I complex... | ACCEPT | Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A |
| GO:0000813 ESCRT I complex | IPI PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo... | ACCEPT | Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A |
| GO:0010008 endosome membrane | NAS PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo... | ACCEPT | Summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments |
| GO:0036258 multivesicular body assembly | NAS PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo... | ACCEPT | Summary: multivesicular body assembly is supported as part of the core VPS37B/ESCRT-I endosomal cargo-sorting role. Reason: VPS37B/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting and MVB biogenesis. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway | NAS PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo... | ACCEPT | Summary: protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway is supported as part of the core VPS37B/ESCRT-I endosomal cargo-sorting role. Reason: VPS37B/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting and MVB biogenesis. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0090148 membrane fission | NAS PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo... | ACCEPT | Summary: Membrane fission is supported for ESCRT-I reverse-topology membrane remodeling. Reason: VPS37B is part of the human ESCRT-I headpiece structure, and ESCRT-I scaffolds reverse-topology membrane remodeling upstream of ESCRT-III. Supporting Evidence: PMID:20588296 ESCRT-I and ESCRT-II direct membrane budding away from the cytosol PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topology membrane scission PMID:32424346 ESCRT-I is not merely a bridging adaptor |
| GO:0005515 protein binding | IPI PMID:18005716 Identification of human MVB12 proteins as ESCRT-I subunits t... | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0016236 macroautophagy | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | MARK AS OVER ANNOTATED | Summary: Macroautophagy is over-annotated for VPS37B. Reason: The accessible ESCRT autophagy evidence is broad or VPS37A-specific; there is not enough direct evidence to make VPS37B a phagophore-closure/autophagosome assembly factor. Proposed replacements: ESCRT I complex membrane fission Supporting Evidence: PMID:20588296 viral budding, cytokinesis and, probably, autophagy PMID:31519728 the ESCRT-I subunit VPS37A as a critical component for phagophore closure PMID:31519728 required for autophagosome completion but dispensable for ESCRT-I complex formation |
| GO:0000813 ESCRT I complex | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | ACCEPT | Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A |
| GO:0036258 multivesicular body assembly | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | ACCEPT | Summary: multivesicular body assembly is supported as part of the core VPS37B/ESCRT-I endosomal cargo-sorting role. Reason: VPS37B/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting and MVB biogenesis. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0039702 viral budding via host ESCRT complex | TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a... | KEEP AS NON CORE | Summary: viral budding via host ESCRT complex is supported as a secondary context but is not the core VPS37B proteostasis function. Reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy |
| GO:0000813 ESCRT I complex | IDA PMID:18005716 Identification of human MVB12 proteins as ESCRT-I subunits t... | ACCEPT | Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A |
| GO:0005768 endosome | IDA PMID:17940959 Involvement of vacuolar protein sorting pathway in Ebola vir... | ACCEPT | Summary: endosome localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments |
| GO:0005886 plasma membrane | IDA PMID:17940959 Involvement of vacuolar protein sorting pathway in Ebola vir... | KEEP AS NON CORE | Summary: plasma membrane is supported as a secondary context but is not the core VPS37B proteostasis function. Reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy |
| GO:1903774 positive regulation of viral budding via host ESCRT complex | IMP PMID:15218037 The human endosomal sorting complex required for transport (... | KEEP AS NON CORE | Summary: positive regulation of viral budding via host ESCRT complex is supported as a secondary context but is not the core VPS37B proteostasis function. Reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy |
| GO:0005515 protein binding | IPI PMID:15218037 The human endosomal sorting complex required for transport (... | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
| GO:0005737 cytoplasm | IDA PMID:15218037 The human endosomal sorting complex required for transport (... | KEEP AS NON CORE | Summary: cytoplasm is supported as a secondary context but is not the core VPS37B proteostasis function. Reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy |
| GO:0010008 endosome membrane | IDA PMID:15218037 The human endosomal sorting complex required for transport (... | ACCEPT | Summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments |
| GO:0019076 viral release from host cell | IMP PMID:15218037 The human endosomal sorting complex required for transport (... | KEEP AS NON CORE | Summary: viral release from host cell is supported as a secondary context but is not the core VPS37B proteostasis function. Reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy |
| GO:0048306 calcium-dependent protein binding | IPI PMID:23924735 VPS37 isoforms differentially modulate the ternary complex f... | KEEP AS NON CORE | Summary: Calcium-dependent protein binding is supported but secondary. Reason: VPS37B participates in an ALG-2/ALIX/ESCRT-I calcium-dependent ternary complex, but this is not the core VPS37B proteostasis function. Supporting Evidence: PMID:23924735 VPS37B and VPS37C appeared to interact with ALG-2 PMID:23924735 adaptor protein that bridges ALIX and ESCRT-I |
| GO:0070062 extracellular exosome | HDA PMID:23533145 In-depth proteomic analyses of exosomes isolated from expres... | KEEP AS NON CORE | Summary: extracellular exosome is supported as a secondary context but is not the core VPS37B proteostasis function. Reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy |
| GO:0000813 ESCRT I complex | IDA PMID:22232651 Structural basis for membrane targeting by the MVB12-associa... | ACCEPT | Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A |
| GO:0070062 extracellular exosome | HDA PMID:19056867 Large-scale proteomics and phosphoproteomics of urinary exos... | KEEP AS NON CORE | Summary: extracellular exosome is supported as a secondary context but is not the core VPS37B proteostasis function. Reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy |
| GO:0000813 ESCRT I complex | IDA PMID:22405001 The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a ... | ACCEPT | Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A |
| GO:0010008 endosome membrane | TAS Reactome:R-HSA-184269 | ACCEPT | Summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments |
| GO:0010008 endosome membrane | TAS Reactome:R-HSA-3149434 | ACCEPT | Summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments |
| GO:0010008 endosome membrane | TAS Reactome:R-HSA-3159232 | ACCEPT | Summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments |
| GO:0010008 endosome membrane | TAS Reactome:R-HSA-917696 | ACCEPT | Summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments |
| GO:0010008 endosome membrane | TAS Reactome:R-HSA-917730 | ACCEPT | Summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments |
| GO:0030496 midbody | IDA PMID:19129479 Biochemical analyses of human IST1 and its function in cytok... | KEEP AS NON CORE | Summary: Midbody localization is a secondary ESCRT/cytokinesis context and is not core for VPS37B. Reason: ESCRT machinery functions in cytokinesis, and VPS37B interacts with IST1, but the core proteostasis review focus is endosomal ESCRT-I sorting and membrane remodeling. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy |
| GO:0005515 protein binding | IPI PMID:19129479 Biochemical analyses of human IST1 and its function in cytok... | MARK AS OVER ANNOTATED | Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis |
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Download this section (compressed HTML)Q: Should VPS37B macroautophagy annotations be retired or kept non-core given that the direct phagophore-closure evidence is VPS37A-specific?
Suggested experts: GO autophagy editors, GO ESCRT curators
Q: Should generic VPS37B protein-binding annotations be replaced by ESCRT-I complex membership and specific endosomal sorting terms?
Suggested experts: GO molecular function editors, UniProt curators
Experiment: Compare VPS37A, VPS37B, and VPS37C depletion/rescue in HT-LC3 autophagosome closure assays and parallel EGFR MVB-sorting assays, using endogenous expression or matched rescue levels.
Hypothesis: VPS37A, but not VPS37B, is the VPS37 paralog specialized for phagophore closure because VPS37B lacks the VPS37A PUEV-dependent targeting function.
Type: VPS37 paralog phagophore closure comparison
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