Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	IBA GO_REF:0000033	ACCEPT	Summary: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway is supported as part of the core VPS37B/ESCRT-I endosomal cargo-sorting role. Reason: VPS37B/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting and MVB biogenesis. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0000813 ESCRT I complex	IBA GO_REF:0000033	ACCEPT	Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A
GO:0006612 protein targeting to membrane	IBA GO_REF:0000033	MODIFY	Summary: protein targeting to membrane is too broad for VPS37B ESCRT-I function. Reason: The supported process is ESCRT-I-dependent endosomal cargo sorting into the MVB pathway, not generic protein targeting. Proposed replacements: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway multivesicular body assembly Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0006623 protein targeting to vacuole	IBA GO_REF:0000033	MODIFY	Summary: protein targeting to vacuole is too broad for VPS37B ESCRT-I function. Reason: The supported process is ESCRT-I-dependent endosomal cargo sorting into the MVB pathway, not generic protein targeting. Proposed replacements: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway multivesicular body assembly Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0000813 ESCRT I complex	IEA GO_REF:0000117	ACCEPT	Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A
GO:0016236 macroautophagy	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: Macroautophagy is over-annotated for VPS37B. Reason: The accessible ESCRT autophagy evidence is broad or VPS37A-specific; there is not enough direct evidence to make VPS37B a phagophore-closure/autophagosome assembly factor. Proposed replacements: ESCRT I complex membrane fission Supporting Evidence: PMID:20588296 viral budding, cytokinesis and, probably, autophagy PMID:31519728 the ESCRT-I subunit VPS37A as a critical component for phagophore closure PMID:31519728 required for autophagosome completion but dispensable for ESCRT-I complex formation
GO:0031902 late endosome membrane	IEA GO_REF:0000044	ACCEPT	Summary: late endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments
GO:0036258 multivesicular body assembly	IEA GO_REF:0000117	ACCEPT	Summary: multivesicular body assembly is supported as part of the core VPS37B/ESCRT-I endosomal cargo-sorting role. Reason: VPS37B/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting and MVB biogenesis. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0039702 viral budding via host ESCRT complex	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: viral budding via host ESCRT complex is supported as a secondary context but is not the core VPS37B proteostasis function. Reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy
GO:0048306 calcium-dependent protein binding	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: Calcium-dependent protein binding is supported but secondary. Reason: VPS37B participates in an ALG-2/ALIX/ESCRT-I calcium-dependent ternary complex, but this is not the core VPS37B proteostasis function. Supporting Evidence: PMID:23924735 VPS37B and VPS37C appeared to interact with ALG-2 PMID:23924735 adaptor protein that bridges ALIX and ESCRT-I
GO:0005515 protein binding	IPI PMID:21516116 Next-generation sequencing to generate interactome datasets.	MARK AS OVER ANNOTATED	Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0005515 protein binding	IPI PMID:25416956 A proteome-scale map of the human interactome network.	MARK AS OVER ANNOTATED	Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0005515 protein binding	IPI PMID:26871637 Widespread Expansion of Protein Interaction Capabilities by ...	MARK AS OVER ANNOTATED	Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0005515 protein binding	IPI PMID:28514442 Architecture of the human interactome defines protein commun...	MARK AS OVER ANNOTATED	Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0005515 protein binding	IPI PMID:31515488 Extensive disruption of protein interactions by genetic vari...	MARK AS OVER ANNOTATED	Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0005515 protein binding	IPI PMID:32296183 A reference map of the human binary protein interactome.	MARK AS OVER ANNOTATED	Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0005515 protein binding	IPI PMID:33961781 Dual proteome-scale networks reveal cell-specific remodeling...	MARK AS OVER ANNOTATED	Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0005515 protein binding	IPI PMID:35271311 OpenCell: Endogenous tagging for the cartography of human ce...	MARK AS OVER ANNOTATED	Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0000813 ESCRT I complex	IPI PMID:18005716 Identification of human MVB12 proteins as ESCRT-I subunits t...	ACCEPT	Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A
GO:0000813 ESCRT I complex	IPI PMID:21757351 UBAP1 is a component of an endosome-specific ESCRT-I complex...	ACCEPT	Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A
GO:0000813 ESCRT I complex	IPI PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo...	ACCEPT	Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A
GO:0010008 endosome membrane	NAS PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo...	ACCEPT	Summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments
GO:0036258 multivesicular body assembly	NAS PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo...	ACCEPT	Summary: multivesicular body assembly is supported as part of the core VPS37B/ESCRT-I endosomal cargo-sorting role. Reason: VPS37B/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting and MVB biogenesis. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	NAS PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo...	ACCEPT	Summary: protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway is supported as part of the core VPS37B/ESCRT-I endosomal cargo-sorting role. Reason: VPS37B/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting and MVB biogenesis. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0090148 membrane fission	NAS PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topolo...	ACCEPT	Summary: Membrane fission is supported for ESCRT-I reverse-topology membrane remodeling. Reason: VPS37B is part of the human ESCRT-I headpiece structure, and ESCRT-I scaffolds reverse-topology membrane remodeling upstream of ESCRT-III. Supporting Evidence: PMID:20588296 ESCRT-I and ESCRT-II direct membrane budding away from the cytosol PMID:32424346 A helical assembly of human ESCRT-I scaffolds reverse-topology membrane scission PMID:32424346 ESCRT-I is not merely a bridging adaptor
GO:0005515 protein binding	IPI PMID:18005716 Identification of human MVB12 proteins as ESCRT-I subunits t...	MARK AS OVER ANNOTATED	Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0016236 macroautophagy	TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a...	MARK AS OVER ANNOTATED	Summary: Macroautophagy is over-annotated for VPS37B. Reason: The accessible ESCRT autophagy evidence is broad or VPS37A-specific; there is not enough direct evidence to make VPS37B a phagophore-closure/autophagosome assembly factor. Proposed replacements: ESCRT I complex membrane fission Supporting Evidence: PMID:20588296 viral budding, cytokinesis and, probably, autophagy PMID:31519728 the ESCRT-I subunit VPS37A as a critical component for phagophore closure PMID:31519728 required for autophagosome completion but dispensable for ESCRT-I complex formation
GO:0000813 ESCRT I complex	TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a...	ACCEPT	Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A
GO:0036258 multivesicular body assembly	TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a...	ACCEPT	Summary: multivesicular body assembly is supported as part of the core VPS37B/ESCRT-I endosomal cargo-sorting role. Reason: VPS37B/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting and MVB biogenesis. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0039702 viral budding via host ESCRT complex	TAS PMID:20588296 Membrane budding and scission by the ESCRT machinery: it's a...	KEEP AS NON CORE	Summary: viral budding via host ESCRT complex is supported as a secondary context but is not the core VPS37B proteostasis function. Reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy
GO:0000813 ESCRT I complex	IDA PMID:18005716 Identification of human MVB12 proteins as ESCRT-I subunits t...	ACCEPT	Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A
GO:0005768 endosome	IDA PMID:17940959 Involvement of vacuolar protein sorting pathway in Ebola vir...	ACCEPT	Summary: endosome localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments
GO:0005886 plasma membrane	IDA PMID:17940959 Involvement of vacuolar protein sorting pathway in Ebola vir...	KEEP AS NON CORE	Summary: plasma membrane is supported as a secondary context but is not the core VPS37B proteostasis function. Reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy
GO:1903774 positive regulation of viral budding via host ESCRT complex	IMP PMID:15218037 The human endosomal sorting complex required for transport (...	KEEP AS NON CORE	Summary: positive regulation of viral budding via host ESCRT complex is supported as a secondary context but is not the core VPS37B proteostasis function. Reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy
GO:0005515 protein binding	IPI PMID:15218037 The human endosomal sorting complex required for transport (...	MARK AS OVER ANNOTATED	Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis
GO:0005737 cytoplasm	IDA PMID:15218037 The human endosomal sorting complex required for transport (...	KEEP AS NON CORE	Summary: cytoplasm is supported as a secondary context but is not the core VPS37B proteostasis function. Reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy
GO:0010008 endosome membrane	IDA PMID:15218037 The human endosomal sorting complex required for transport (...	ACCEPT	Summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments
GO:0019076 viral release from host cell	IMP PMID:15218037 The human endosomal sorting complex required for transport (...	KEEP AS NON CORE	Summary: viral release from host cell is supported as a secondary context but is not the core VPS37B proteostasis function. Reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy
GO:0048306 calcium-dependent protein binding	IPI PMID:23924735 VPS37 isoforms differentially modulate the ternary complex f...	KEEP AS NON CORE	Summary: Calcium-dependent protein binding is supported but secondary. Reason: VPS37B participates in an ALG-2/ALIX/ESCRT-I calcium-dependent ternary complex, but this is not the core VPS37B proteostasis function. Supporting Evidence: PMID:23924735 VPS37B and VPS37C appeared to interact with ALG-2 PMID:23924735 adaptor protein that bridges ALIX and ESCRT-I
GO:0070062 extracellular exosome	HDA PMID:23533145 In-depth proteomic analyses of exosomes isolated from expres...	KEEP AS NON CORE	Summary: extracellular exosome is supported as a secondary context but is not the core VPS37B proteostasis function. Reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy
GO:0000813 ESCRT I complex	IDA PMID:22232651 Structural basis for membrane targeting by the MVB12-associa...	ACCEPT	Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A
GO:0070062 extracellular exosome	HDA PMID:19056867 Large-scale proteomics and phosphoproteomics of urinary exos...	KEEP AS NON CORE	Summary: extracellular exosome is supported as a secondary context but is not the core VPS37B proteostasis function. Reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy
GO:0000813 ESCRT I complex	IDA PMID:22405001 The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a ...	ACCEPT	Summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation. Reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A
GO:0010008 endosome membrane	TAS Reactome:R-HSA-184269	ACCEPT	Summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments
GO:0010008 endosome membrane	TAS Reactome:R-HSA-3149434	ACCEPT	Summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments
GO:0010008 endosome membrane	TAS Reactome:R-HSA-3159232	ACCEPT	Summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments
GO:0010008 endosome membrane	TAS Reactome:R-HSA-917696	ACCEPT	Summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments
GO:0010008 endosome membrane	TAS Reactome:R-HSA-917730	ACCEPT	Summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function. Reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal membranes in the VPS4-dependent ESCRT cycle. Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Late endosome membrane file:human/VPS37B/VPS37B-uniprot.txt Recruited to the endosomal membrane PMID:15218037 VPS37B became trapped on aberrant endosomal compartments
GO:0030496 midbody	IDA PMID:19129479 Biochemical analyses of human IST1 and its function in cytok...	KEEP AS NON CORE	Summary: Midbody localization is a secondary ESCRT/cytokinesis context and is not core for VPS37B. Reason: ESCRT machinery functions in cytokinesis, and VPS37B interacts with IST1, but the core proteostasis review focus is endosomal ESCRT-I sorting and membrane remodeling. Supporting Evidence: PMID:15218037 recruit TSG101/ESCRT-I activity and thereby rescue the budding PMID:18005716 plays essential roles in HIV budding and endosomal protein sorting PMID:19129479 functions in the terminal stages of cytokinesis PMID:20588296 viral budding, cytokinesis and, probably, autophagy
GO:0005515 protein binding	IPI PMID:19129479 Biochemical analyses of human IST1 and its function in cytok...	MARK AS OVER ANNOTATED	Summary: Protein binding is too generic to represent VPS37B function. Reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal sorting/membrane remodeling, not generic protein binding from interaction assays. Proposed replacements: ESCRT I complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Supporting Evidence: file:human/VPS37B/VPS37B-uniprot.txt Component of the ESCRT-I complex file:human/VPS37B/VPS37B-uniprot.txt which consists of TSG101, VPS28, a VPS37 file:human/VPS37B/VPS37B-uniprot.txt Interacts with TSG101, VPS28, MVB12A and MVB12B PMID:15218037 VPS37A and VPS37B bind TSG101 PMID:15218037 TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG PMID:18005716 All ESCRT-I complexes contain three PMID:32424346 comprising TSG101-VPS28-VPS37B-MVB12A file:human/VPS37B/VPS37B-uniprot.txt Required for the sorting of endocytic file:human/VPS37B/VPS37B-uniprot.txt ubiquitinated cargos into multivesicular bodies PMID:15218037 sorting ubiquitylated protein cargoes into multivesicular bodies PMID:21757351 required for sorting EGFR to the MVB PMID:22405001 endosomal sorting of ubiquitinated cargo, MVB biogenesis

Core Functions

VPS37B is a structural ESCRT-I subunit that helps organize the TSG101-VPS28-VPS37-MVB12/UBAP complex for ubiquitin-dependent endosomal cargo sorting, MVB assembly, and ESCRT-I-linked reverse-topology membrane remodeling.

Directly Involved In:

ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway multivesicular body assembly membrane fission

Cellular Locations:

endosome membrane late endosome membrane endosome

In Complex:

ESCRT I complex

Supporting Evidence:

file:human/VPS37B/VPS37B-uniprot.txt
Component of the ESCRT-I complex
file:human/VPS37B/VPS37B-uniprot.txt
which consists of TSG101, VPS28, a VPS37
file:human/VPS37B/VPS37B-uniprot.txt
Interacts with TSG101, VPS28, MVB12A and MVB12B
PMID:15218037
VPS37A and VPS37B bind TSG101
PMID:15218037
TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG
PMID:18005716
All ESCRT-I complexes contain three
PMID:32424346
comprising TSG101-VPS28-VPS37B-MVB12A
file:human/VPS37B/VPS37B-uniprot.txt
Required for the sorting of endocytic
file:human/VPS37B/VPS37B-uniprot.txt
ubiquitinated cargos into multivesicular bodies
PMID:15218037
sorting ubiquitylated protein cargoes into multivesicular bodies
PMID:21757351
required for sorting EGFR to the MVB
PMID:22405001
endosomal sorting of ubiquitinated cargo, MVB biogenesis
file:human/VPS37B/VPS37B-uniprot.txt
Late endosome membrane
file:human/VPS37B/VPS37B-uniprot.txt
Recruited to the endosomal membrane
PMID:15218037
VPS37B became trapped on aberrant endosomal compartments
PMID:20588296
ESCRT-I and ESCRT-II direct membrane budding away from the cytosol
PMID:32424346
A helical assembly of human ESCRT-I scaffolds reverse-topology membrane scission
PMID:32424346
ESCRT-I is not merely a bridging adaptor

References

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

PMID:15218037

The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding.

PMID:17940959

Involvement of vacuolar protein sorting pathway in Ebola virus release independent of TSG101 interaction.

PMID:18005716

Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding.

PMID:19056867

Large-scale proteomics and phosphoproteomics of urinary exosomes.

PMID:19129479

Biochemical analyses of human IST1 and its function in cytokinesis.

PMID:20588296

Membrane budding and scission by the ESCRT machinery: it's all in the neck.

PMID:21516116

Next-generation sequencing to generate interactome datasets.

PMID:21757351

UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.

PMID:22232651

Structural basis for membrane targeting by the MVB12-associated β-prism domain of the human ESCRT-I MVB12 subunit.

PMID:22405001

The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain.

PMID:23533145

In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.

PMID:23924735

VPS37 isoforms differentially modulate the ternary complex formation of ALIX, ALG-2, and ESCRT-I.

PMID:25416956

A proteome-scale map of the human interactome network.

PMID:26871637

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.

PMID:28514442

Architecture of the human interactome defines protein communities and disease networks.

PMID:31515488

Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.

PMID:32296183

A reference map of the human binary protein interactome.

PMID:32424346

A helical assembly of human ESCRT-I scaffolds reverse-topology membrane scission.

PMID:33961781

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

PMID:35271311

OpenCell: Endogenous tagging for the cartography of human cellular organization.

Reactome:R-HSA-184269

Monoubiquitinated N-myristoyl GAG polyprotein is targeted to the late endosomal vesicle membrane by the ESCRT-I complex

Reactome:R-HSA-3149434

Transport of GAG to the Plasma Membrane

Reactome:R-HSA-3159232

Recruitment Of HIV Virion Budding Machinery

Reactome:R-HSA-917696

Cargo Sequestration

Reactome:R-HSA-917730

Cargo Recognition And Sorting

PMID:31519728

VPS37A directs ESCRT recruitment for phagophore closure.

file:human/VPS37B/VPS37B-uniprot.txt

UniProtKB record for human VPS37B

file:human/VPS37B/VPS37B-notes.md

VPS37B review notes

Suggested Experiments

Experiment: Compare VPS37A, VPS37B, and VPS37C depletion/rescue in HT-LC3 autophagosome closure assays and parallel EGFR MVB-sorting assays, using endogenous expression or matched rescue levels.

Hypothesis: VPS37A, but not VPS37B, is the VPS37 paralog specialized for phagophore closure because VPS37B lacks the VPS37A PUEV-dependent targeting function.

Type: VPS37 paralog phagophore closure comparison

📚 Additional Documentation

Notes

(VPS37B-notes.md)

VPS37B review notes

Scope

VPS37B is reviewed in the PN ESCRT-I branch. PN entries without PMIDs were used as context only. The direct phagophore-closure evidence in this ESCRT-I neighborhood is VPS37A-specific; for VPS37B the supported core is ESCRT-I membership, endosomal MVB cargo sorting, and ESCRT-I membrane-remodeling context.

Evidence synthesis

VPS37B is a VPS37-family ESCRT-I subunit. UniProt describes it as a "Component of the ESCRT-I complex" and says it is "Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies" [file:human/VPS37B/VPS37B-uniprot.txt, "Component of the ESCRT-I complex"; file:human/VPS37B/VPS37B-uniprot.txt, "Required for the sorting of endocytic"]. UniProt also states that the ESCRT-I complex "consists of TSG101, VPS28, a VPS37 protein" and that VPS37B interacts with TSG101, VPS28, MVB12A, and MVB12B [file:human/VPS37B/VPS37B-uniprot.txt, "which consists of TSG101, VPS28, a VPS37"; file:human/VPS37B/VPS37B-uniprot.txt, "Interacts with TSG101, VPS28, MVB12A and MVB12B"]. This supports accepting ESCRT-I complex and MVB sorting rows.

The core VPS37B experimental paper defines it as a human Vps37 ortholog in ESCRT-I. The abstract reports that "VPS37A and VPS37B bind TSG101", that "TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG", that the proteins "comigrated together in soluble complexes of the correct size for human ESCRT-I", and that VPS37B becomes trapped on aberrant endosomal compartments in the presence of ATPase-defective VPS4A [PMID:15218037, "VPS37A and VPS37B bind TSG101"; PMID:15218037, "TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG"; PMID:15218037, "complexes of the correct size for human ESCRT-I"; PMID:15218037, "VPS37B became trapped on aberrant endosomal compartments"]. This supports endosome/endosome membrane localization and ESCRT-I complex membership. The same paper also supports viral budding, but that is a non-core host-pathogen context for this proteostasis review.

Structural ESCRT-I evidence supports VPS37B as part of the ESCRT-I headpiece. The human ESCRT-I structural paper describes the headpiece as "comprising TSG101-VPS28-VPS37B-MVB12A" and says ESCRT-I is "not merely a bridging adaptor" but forms assemblies that template membrane remodeling [PMID:32424346, "comprising TSG101-VPS28-VPS37B-MVB12A"; PMID:32424346, "ESCRT-I is not merely a bridging adaptor"]. This supports accepting membrane fission as a complex-level ESCRT-I remodeling annotation, but it does not make VPS37B itself a directly tested autophagosome-closure factor.

The macroautophagy rows should be treated cautiously. PMID:20588296 says ESCRT-III-mediated neck cleavage is important for MVBs, viral budding, cytokinesis, and "probably, autophagy" [PMID:20588296, "viral budding, cytokinesis and, probably, autophagy"]. The direct mammalian phagophore-closure study identifies VPS37A as the VPS37 subunit required for phagophore closure and says VPS37A is "required for autophagosome completion but dispensable for ESCRT-I complex formation" [PMID:31519728, "the ESCRT-I subunit VPS37A as a critical component for phagophore closure"; PMID:31519728, "required for autophagosome completion but dispensable for ESCRT-I complex formation"]. This argues against transferring the autophagosome assembly conclusion to VPS37B without additional evidence.

VPS37B and VPS37C have a secondary calcium-dependent ALIX/ALG-2 interaction context. PMID:23924735 reports that VPS37B and VPS37C interact with ALG-2 more strongly than TSG101 and that ALG-2 acts as a Ca2+-dependent adaptor bridging ALIX and ESCRT-I [PMID:23924735, "VPS37B and VPS37C appeared to interact with ALG-2"; PMID:23924735, "Ca²⁺-dependent adaptor protein that bridges ALIX and ESCRT-I"]. This supports keeping calcium-dependent protein binding as non-core rather than making it the main molecular function.

Generic protein binding rows are over-annotated. The meaningful curation targets are ESCRT-I complex membership, MVB/endosomal sorting, and ESCRT-I membrane remodeling. Viral budding, viral release, positive regulation of viral budding, exosome, plasma membrane, cytoplasm, and midbody rows are supported contexts but should remain non-core for the proteostasis review.

Falcon

Falcon deep research was started for VPS37B on 2026-06-02 but timed out after 600 seconds and did not produce a usable VPS37B-deep-research-falcon.md report. The review therefore relies on the local UniProt, GOA, cached-publication, and PN-context evidence summarized above.

Pn Notes

(VPS37B-pn-notes.md)

VPS37B PN Consistency Notes

Generated: 2026-06-18
Project: PROTEOSTASIS
Scope: PN consistency rereview against local AIGR review and available deep-research artifacts
UniProt: Q9H9H4
AIGR review status: COMPLETE
Review batch: proteostasis-pr-1217 (PR 1217)
Batch change status: added

Source Files Checked

AIGR review YAML: present - genes/human/VPS37B/VPS37B-ai-review.yaml
AIGR review HTML: present - genes/human/VPS37B/VPS37B-ai-review.html
Gene notes: present - genes/human/VPS37B/VPS37B-notes.md
GOA TSV: present - genes/human/VPS37B/VPS37B-goa.tsv
UniProt record: present - genes/human/VPS37B/VPS37B-uniprot.txt
PN dossier: projects/PROTEOSTASIS/reports/phase1_dossiers/VPS37B.md
PN consistency section: projects/PROTEOSTASIS/reports/phase1_dossiers/_sections/VPS37B.md
PN projection report: projects/PROTEOSTASIS/reports/pn_projection/pn_projected_gene_go_summary.tsv
PN mapping audit: projects/PROTEOSTASIS/reports/pn_mapping_audit/current_mapping_scrutiny.tsv

Deep Research Files

No *-deep-research*.md file found in this gene directory.

AIGR Review Snapshot

Description: VPS37B is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and MVB12/UBAP-family subunits. It supports ESCRT-I-dependent endosomal sorting of ubiquitinated cargo into multivesicular bodies and contributes to ESCRT-I membrane-remodeling contexts. Current local evidence does not support transferring the VPS37A-specific phagophore-closure role to VPS37B as a core annotation.
Existing/core annotation action counts: ACCEPT: 24; KEEP_AS_NON_CORE: 11; MARK_AS_OVER_ANNOTATED: 13; MODIFY: 2

PN Consistency Summary

Consistency: PARTIAL CONTRADICTION. ESCRT-I membership is fully consistent (review ACCEPTs GO:0000813 across many evidence lines; deep notes confirm TSG101/VPS28 co-IP, PMID:15218037; structural headpiece PMID:32424346). But on autophagy the review and PN diverge: the review MARK_AS_OVER_ANNOTATED the GO:0016236 macroautophagy rows and does NOT add GO:0000045, explicitly stating the phagophore-closure role is VPS37A-specific and should not be transferred to VPS37B. PN nonetheless projects GO:0000045 to VPS37B via the shared sealing group.
PN story / NEW pressure: Over-reaches for autophagy. PN's group-level GO:0000045 projection asserts an autophagosome-assembly role the review judges unsupported for VPS37B (no gene-specific phagophore-closure data; only "probably autophagy" review-level statements). ESCRT-I (GO:0000813) is correctly already captured. No defensible NEW term beyond GO:0000813.
Evidence alignment: Partial. PN cites only PMID:32424346 (ESCRT-I scaffold), which the review uses for complex membership but explicitly notes used a VPS37B headpiece for general mechanism, not VPS37B autophagy. Review adds PMID:15218037, 23924735 (ALG-2) not in PN.
Verdict: Consistent on ESCRT-I; GO:0000045 projection over-reaches. Recommended edits: none to YAML (review correctly omits GO:0000045); flag PN sealing-group→GO:0000045 as non-propagating to VPS37B.

Full Consistency Review

UniProt: Q9H9H4 · batch: proteostasis-pr-1217 · review status: COMPLETE
PN placement: 1 row, ALP — Autophagosome closure maturation and lysosome fusion → Sealing of autophagophore membrane → ESCRT-I complex component. PN-node mapping: leaf=mapped→GO:0000813 ESCRT I complex; sealing group=mapped→GO:0000045 autophagosome assembly; class=context_only (GO:0016236). Projected: GO:0000045 (more_specific_than_existing_goa), GO:0000813 (already_in_goa_exact).
Consistency: PARTIAL CONTRADICTION. ESCRT-I membership is fully consistent (review ACCEPTs GO:0000813 across many evidence lines; deep notes confirm TSG101/VPS28 co-IP, PMID:15218037; structural headpiece PMID:32424346). But on autophagy the review and PN diverge: the review MARK_AS_OVER_ANNOTATED the GO:0016236 macroautophagy rows and does NOT add GO:0000045, explicitly stating the phagophore-closure role is VPS37A-specific and should not be transferred to VPS37B. PN nonetheless projects GO:0000045 to VPS37B via the shared sealing group.
PN story / NEW pressure: Over-reaches for autophagy. PN's group-level GO:0000045 projection asserts an autophagosome-assembly role the review judges unsupported for VPS37B (no gene-specific phagophore-closure data; only "probably autophagy" review-level statements). ESCRT-I (GO:0000813) is correctly already captured. No defensible NEW term beyond GO:0000813.
Mapping strategy: Flag. The leaf→GO:0000813 mapping is right and harmless (already in GOA). The group→GO:0000045 projection is the issue: it is broader than the review supports for this paralog, paralleling the rejected TOMM20/HSPA8/RAB7A "shared-node over-reach" precedent. GO:0000045 should not propagate to VPS37B on current evidence — restrict the sealing-group projection or mark VPS37B's group membership as non-propagating.
Evidence alignment: Partial. PN cites only PMID:32424346 (ESCRT-I scaffold), which the review uses for complex membership but explicitly notes used a VPS37B headpiece for general mechanism, not VPS37B autophagy. Review adds PMID:15218037, 23924735 (ALG-2) not in PN.
Verdict: Consistent on ESCRT-I; GO:0000045 projection over-reaches. Recommended edits: none to YAML (review correctly omits GO:0000045); flag PN sealing-group→GO:0000045 as non-propagating to VPS37B.

PN Dossier Context

review_batch: proteostasis-pr-1217
review_yaml: genes/human/VPS37B/VPS37B-ai-review.yaml
PN workbook rows: 1

PN row 1: Autophagy-Lysosome Pathway | Autophagosome closure maturation and lysosome fusion | Sealing of autophagophore membrane | ESCRT-I complex component

UniProt: Q9H9H4
In branches: ALP
Notes: Component of the ESCRT-I complex, involved in autophagosome closure
PN references (titles):
- A helical assembly of human ESCRT-I scaffolds reverse-topology membrane scission | Nature Structural & Molecular Biology
PN-node mapping records (path + ancestors):
- [type] Autophagy-Lysosome Pathway|Autophagosome closure maturation and lysosome fusion|Sealing of autophagophore membrane|ESCRT-I complex component
  status=mapped scope=ok_for_propagation_to_go GO=[GO:0000813 ESCRT I complex]
  rationale: This leaf is restricted to ESCRT-I components used in autophagophore sealing. The shared GO assertion is ESCRT I complex membership.
- [group] Autophagy-Lysosome Pathway|Autophagosome closure maturation and lysosome fusion|Sealing of autophagophore membrane
  status=mapped scope=ok_for_propagation_to_go GO=[GO:0000045 autophagosome assembly]
  rationale: This group captures autophagophore closure/sealing, a late step in autophagosome assembly. Autophagosome assembly is the safer process target than autophagosome-lysosome fusion.
- [class] Autophagy-Lysosome Pathway|Autophagosome closure maturation and lysosome fusion
  status=context_only scope=too_broad_to_propagate GO=[GO:0016236 macroautophagy]
  rationale: This class is a late macroautophagy context, but the subtree mixes docking, fusion, localization, membrane-composition, and unknown late-stage roles. The class-level relation is useful for display while propagation is restricted to narrower mechanism nodes.
- [branch] Autophagy-Lysosome Pathway
  status=no_mapping scope= GO=[]
  rationale: Reviewed as the top-level PN branch. It is a project taxonomy umbrella rather than a direct GO assertion; all propagation must come from manually curated child nodes.

Projected GO annotations (2)

GO:0000045 autophagosome assembly | scope=ok_for_propagation_to_go | goa_status=more_specific_than_existing_goa | from=Autophagy-Lysosome Pathway|Autophagosome closure maturation and lysosome fusion|Sealing of autophagophore membrane
GO:0000813 ESCRT I complex | scope=ok_for_propagation_to_go | goa_status=already_in_goa_exact | from=Autophagy-Lysosome Pathway|Autophagosome closure maturation and lysosome fusion|Sealing of autophagophore membrane|ESCRT-I complex component

Note

This file is generated from the current PROTEOSTASIS phase-1 dossier and local gene-review artifacts. Edit the source review, PN mapping, or dossier rather than this generated note when correcting the underlying curation.

📄 View Raw YAML

id: Q9H9H4
gene_symbol: VPS37B
product_type: PROTEIN
status: COMPLETE
taxon:
  id: NCBITaxon:9606
  label: Homo sapiens
description: VPS37B is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and MVB12/UBAP-family
  subunits. It supports ESCRT-I-dependent endosomal sorting of ubiquitinated cargo into multivesicular bodies 
  and contributes to ESCRT-I membrane-remodeling contexts. Current local evidence does not support 
  transferring the VPS37A-specific phagophore-closure role to VPS37B as a core annotation.
existing_annotations:
- term:
    id: GO:0043162
    label: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  review:
    summary: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway is 
      supported as part of the core VPS37B/ESCRT-I endosomal cargo-sorting role.
    action: ACCEPT
    reason: VPS37B/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting and MVB biogenesis.
    additional_reference_ids: &id001
    - PMID:15218037
    - PMID:21757351
    - PMID:22405001
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    supported_by: &id002
    - &id012
      reference_id: file:human/VPS37B/VPS37B-uniprot.txt
      supporting_text: Required for the sorting of endocytic
    - &id013
      reference_id: file:human/VPS37B/VPS37B-uniprot.txt
      supporting_text: ubiquitinated cargos into multivesicular bodies
    - &id014
      reference_id: PMID:15218037
      supporting_text: sorting ubiquitylated protein cargoes into multivesicular bodies
    - &id015
      reference_id: PMID:21757351
      supporting_text: required for sorting EGFR to the MVB
    - &id016
      reference_id: PMID:22405001
      supporting_text: endosomal sorting of ubiquitinated cargo, MVB biogenesis
- term:
    id: GO:0000813
    label: ESCRT I complex
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: part_of
  review:
    summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation.
    action: ACCEPT
    reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes.
    additional_reference_ids: &id003
    - PMID:15218037
    - PMID:18005716
    - PMID:21757351
    - PMID:22405001
    - PMID:32424346
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    supported_by: &id004
    - &id005
      reference_id: file:human/VPS37B/VPS37B-uniprot.txt
      supporting_text: Component of the ESCRT-I complex
    - &id006
      reference_id: file:human/VPS37B/VPS37B-uniprot.txt
      supporting_text: which consists of TSG101, VPS28, a VPS37
    - &id007
      reference_id: file:human/VPS37B/VPS37B-uniprot.txt
      supporting_text: Interacts with TSG101, VPS28, MVB12A and MVB12B
    - &id008
      reference_id: PMID:15218037
      supporting_text: VPS37A and VPS37B bind TSG101
    - &id009
      reference_id: PMID:15218037
      supporting_text: TSG101 and VPS28 co-immunoprecipitated with VPS37B-FLAG
    - &id010
      reference_id: PMID:18005716
      supporting_text: All ESCRT-I complexes contain three
    - &id011
      reference_id: PMID:32424346
      supporting_text: comprising TSG101-VPS28-VPS37B-MVB12A
- term:
    id: GO:0006612
    label: protein targeting to membrane
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  review:
    summary: protein targeting to membrane is too broad for VPS37B ESCRT-I function.
    action: MODIFY
    reason: The supported process is ESCRT-I-dependent endosomal cargo sorting into the MVB pathway, not 
      generic protein targeting.
    proposed_replacement_terms:
    - id: GO:0043162
      label: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
    - id: GO:0036258
      label: multivesicular body assembly
    additional_reference_ids: *id001
    supported_by: *id002
- term:
    id: GO:0006623
    label: protein targeting to vacuole
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: involved_in
  review:
    summary: protein targeting to vacuole is too broad for VPS37B ESCRT-I function.
    action: MODIFY
    reason: The supported process is ESCRT-I-dependent endosomal cargo sorting into the MVB pathway, not 
      generic protein targeting.
    proposed_replacement_terms:
    - id: GO:0043162
      label: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
    - id: GO:0036258
      label: multivesicular body assembly
    additional_reference_ids: *id001
    supported_by: *id002
- term:
    id: GO:0000813
    label: ESCRT I complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: part_of
  review:
    summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation.
    action: ACCEPT
    reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes.
    additional_reference_ids: *id003
    supported_by: *id004
- term:
    id: GO:0016236
    label: macroautophagy
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: involved_in
  review:
    summary: Macroautophagy is over-annotated for VPS37B.
    action: MARK_AS_OVER_ANNOTATED
    reason: The accessible ESCRT autophagy evidence is broad or VPS37A-specific; there is not enough direct 
      evidence to make VPS37B a phagophore-closure/autophagosome assembly factor.
    proposed_replacement_terms:
    - id: GO:0000813
      label: ESCRT I complex
    - id: GO:0090148
      label: membrane fission
    additional_reference_ids: &id020
    - PMID:20588296
    - PMID:31519728
    - PMID:32424346
    - file:human/VPS37B/VPS37B-notes.md
    supported_by: &id021
    - reference_id: PMID:20588296
      supporting_text: viral budding, cytokinesis and, probably, autophagy
    - reference_id: PMID:31519728
      supporting_text: the ESCRT-I subunit VPS37A as a critical component for phagophore closure
    - reference_id: PMID:31519728
      supporting_text: required for autophagosome completion but dispensable for ESCRT-I complex formation
- term:
    id: GO:0031902
    label: late endosome membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  review:
    summary: late endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function.
    action: ACCEPT
    reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal 
      membranes in the VPS4-dependent ESCRT cycle.
    additional_reference_ids: &id018
    - PMID:15218037
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    supported_by: &id019
    - &id026
      reference_id: file:human/VPS37B/VPS37B-uniprot.txt
      supporting_text: Late endosome membrane
    - &id027
      reference_id: file:human/VPS37B/VPS37B-uniprot.txt
      supporting_text: Recruited to the endosomal membrane
    - &id028
      reference_id: PMID:15218037
      supporting_text: VPS37B became trapped on aberrant endosomal compartments
- term:
    id: GO:0036258
    label: multivesicular body assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: involved_in
  review:
    summary: multivesicular body assembly is supported as part of the core VPS37B/ESCRT-I endosomal 
      cargo-sorting role.
    action: ACCEPT
    reason: VPS37B/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting and MVB biogenesis.
    additional_reference_ids: *id001
    supported_by: *id002
- term:
    id: GO:0039702
    label: viral budding via host ESCRT complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: involved_in
  review:
    summary: viral budding via host ESCRT complex is supported as a secondary context but is not the core 
      VPS37B proteostasis function.
    action: KEEP_AS_NON_CORE
    reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect 
      ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role.
    additional_reference_ids: &id022
    - PMID:15218037
    - PMID:18005716
    - PMID:19129479
    - PMID:20588296
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    supported_by: &id023
    - reference_id: PMID:15218037
      supporting_text: recruit TSG101/ESCRT-I activity and thereby rescue the budding
    - reference_id: PMID:18005716
      supporting_text: plays essential roles in HIV budding and endosomal protein sorting
    - reference_id: PMID:19129479
      supporting_text: functions in the terminal stages of cytokinesis
    - reference_id: PMID:20588296
      supporting_text: viral budding, cytokinesis and, probably, autophagy
- term:
    id: GO:0048306
    label: calcium-dependent protein binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  qualifier: enables
  review:
    summary: Calcium-dependent protein binding is supported but secondary.
    action: KEEP_AS_NON_CORE
    reason: VPS37B participates in an ALG-2/ALIX/ESCRT-I calcium-dependent ternary complex, but this is not 
      the core VPS37B proteostasis function.
    additional_reference_ids: &id024
    - PMID:23924735
    - file:human/VPS37B/VPS37B-notes.md
    supported_by: &id025
    - reference_id: PMID:23924735
      supporting_text: VPS37B and VPS37C appeared to interact with ALG-2
    - reference_id: PMID:23924735
      supporting_text: adaptor protein that bridges ALIX and ESCRT-I
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:21516116
  qualifier: enables
  review:
    summary: Protein binding is too generic to represent VPS37B function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal 
      sorting/membrane remodeling, not generic protein binding from interaction assays.
    proposed_replacement_terms: &id017
    - id: GO:0000813
      label: ESCRT I complex
    - id: GO:0043162
      label: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
    additional_reference_ids:
    - PMID:15218037
    - PMID:18005716
    - PMID:21757351
    - PMID:22405001
    - PMID:32424346
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    - PMID:15218037
    - PMID:21757351
    - PMID:22405001
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    supported_by:
    - *id005
    - *id006
    - *id007
    - *id008
    - *id009
    - *id010
    - *id011
    - *id012
    - *id013
    - *id014
    - *id015
    - *id016
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:25416956
  qualifier: enables
  review:
    summary: Protein binding is too generic to represent VPS37B function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal 
      sorting/membrane remodeling, not generic protein binding from interaction assays.
    proposed_replacement_terms: *id017
    additional_reference_ids:
    - PMID:15218037
    - PMID:18005716
    - PMID:21757351
    - PMID:22405001
    - PMID:32424346
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    - PMID:15218037
    - PMID:21757351
    - PMID:22405001
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    supported_by:
    - *id005
    - *id006
    - *id007
    - *id008
    - *id009
    - *id010
    - *id011
    - *id012
    - *id013
    - *id014
    - *id015
    - *id016
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:26871637
  qualifier: enables
  review:
    summary: Protein binding is too generic to represent VPS37B function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal 
      sorting/membrane remodeling, not generic protein binding from interaction assays.
    proposed_replacement_terms: *id017
    additional_reference_ids:
    - PMID:15218037
    - PMID:18005716
    - PMID:21757351
    - PMID:22405001
    - PMID:32424346
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    - PMID:15218037
    - PMID:21757351
    - PMID:22405001
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    supported_by:
    - *id005
    - *id006
    - *id007
    - *id008
    - *id009
    - *id010
    - *id011
    - *id012
    - *id013
    - *id014
    - *id015
    - *id016
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:28514442
  qualifier: enables
  review:
    summary: Protein binding is too generic to represent VPS37B function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal 
      sorting/membrane remodeling, not generic protein binding from interaction assays.
    proposed_replacement_terms: *id017
    additional_reference_ids:
    - PMID:15218037
    - PMID:18005716
    - PMID:21757351
    - PMID:22405001
    - PMID:32424346
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    - PMID:15218037
    - PMID:21757351
    - PMID:22405001
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    supported_by:
    - *id005
    - *id006
    - *id007
    - *id008
    - *id009
    - *id010
    - *id011
    - *id012
    - *id013
    - *id014
    - *id015
    - *id016
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:31515488
  qualifier: enables
  review:
    summary: Protein binding is too generic to represent VPS37B function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal 
      sorting/membrane remodeling, not generic protein binding from interaction assays.
    proposed_replacement_terms: *id017
    additional_reference_ids:
    - PMID:15218037
    - PMID:18005716
    - PMID:21757351
    - PMID:22405001
    - PMID:32424346
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    - PMID:15218037
    - PMID:21757351
    - PMID:22405001
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    supported_by:
    - *id005
    - *id006
    - *id007
    - *id008
    - *id009
    - *id010
    - *id011
    - *id012
    - *id013
    - *id014
    - *id015
    - *id016
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:32296183
  qualifier: enables
  review:
    summary: Protein binding is too generic to represent VPS37B function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal 
      sorting/membrane remodeling, not generic protein binding from interaction assays.
    proposed_replacement_terms: *id017
    additional_reference_ids:
    - PMID:15218037
    - PMID:18005716
    - PMID:21757351
    - PMID:22405001
    - PMID:32424346
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    - PMID:15218037
    - PMID:21757351
    - PMID:22405001
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    supported_by:
    - *id005
    - *id006
    - *id007
    - *id008
    - *id009
    - *id010
    - *id011
    - *id012
    - *id013
    - *id014
    - *id015
    - *id016
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:33961781
  qualifier: enables
  review:
    summary: Protein binding is too generic to represent VPS37B function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal 
      sorting/membrane remodeling, not generic protein binding from interaction assays.
    proposed_replacement_terms: *id017
    additional_reference_ids:
    - PMID:15218037
    - PMID:18005716
    - PMID:21757351
    - PMID:22405001
    - PMID:32424346
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    - PMID:15218037
    - PMID:21757351
    - PMID:22405001
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    supported_by:
    - *id005
    - *id006
    - *id007
    - *id008
    - *id009
    - *id010
    - *id011
    - *id012
    - *id013
    - *id014
    - *id015
    - *id016
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:35271311
  qualifier: enables
  review:
    summary: Protein binding is too generic to represent VPS37B function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal 
      sorting/membrane remodeling, not generic protein binding from interaction assays.
    proposed_replacement_terms: *id017
    additional_reference_ids:
    - PMID:15218037
    - PMID:18005716
    - PMID:21757351
    - PMID:22405001
    - PMID:32424346
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    - PMID:15218037
    - PMID:21757351
    - PMID:22405001
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    supported_by:
    - *id005
    - *id006
    - *id007
    - *id008
    - *id009
    - *id010
    - *id011
    - *id012
    - *id013
    - *id014
    - *id015
    - *id016
- term:
    id: GO:0000813
    label: ESCRT I complex
  evidence_type: IPI
  original_reference_id: PMID:18005716
  qualifier: part_of
  review:
    summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation.
    action: ACCEPT
    reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes.
    additional_reference_ids: *id003
    supported_by: *id004
- term:
    id: GO:0000813
    label: ESCRT I complex
  evidence_type: IPI
  original_reference_id: PMID:21757351
  qualifier: part_of
  review:
    summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation.
    action: ACCEPT
    reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes.
    additional_reference_ids: *id003
    supported_by: *id004
- term:
    id: GO:0000813
    label: ESCRT I complex
  evidence_type: IPI
  original_reference_id: PMID:32424346
  qualifier: part_of
  review:
    summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation.
    action: ACCEPT
    reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes.
    additional_reference_ids: *id003
    supported_by: *id004
- term:
    id: GO:0010008
    label: endosome membrane
  evidence_type: NAS
  original_reference_id: PMID:32424346
  qualifier: located_in
  review:
    summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function.
    action: ACCEPT
    reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal 
      membranes in the VPS4-dependent ESCRT cycle.
    additional_reference_ids: *id018
    supported_by: *id019
- term:
    id: GO:0036258
    label: multivesicular body assembly
  evidence_type: NAS
  original_reference_id: PMID:32424346
  qualifier: involved_in
  review:
    summary: multivesicular body assembly is supported as part of the core VPS37B/ESCRT-I endosomal 
      cargo-sorting role.
    action: ACCEPT
    reason: VPS37B/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting and MVB biogenesis.
    additional_reference_ids: *id001
    supported_by: *id002
- term:
    id: GO:0043328
    label: protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the 
      multivesicular body sorting pathway
  evidence_type: NAS
  original_reference_id: PMID:32424346
  qualifier: involved_in
  review:
    summary: protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the 
      multivesicular body sorting pathway is supported as part of the core VPS37B/ESCRT-I endosomal 
      cargo-sorting role.
    action: ACCEPT
    reason: VPS37B/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting and MVB biogenesis.
    additional_reference_ids: *id001
    supported_by: *id002
- term:
    id: GO:0090148
    label: membrane fission
  evidence_type: NAS
  original_reference_id: PMID:32424346
  qualifier: involved_in
  review:
    summary: Membrane fission is supported for ESCRT-I reverse-topology membrane remodeling.
    action: ACCEPT
    reason: VPS37B is part of the human ESCRT-I headpiece structure, and ESCRT-I scaffolds reverse-topology 
      membrane remodeling upstream of ESCRT-III.
    additional_reference_ids:
    - PMID:20588296
    - PMID:32424346
    - file:human/VPS37B/VPS37B-notes.md
    supported_by:
    - &id029
      reference_id: PMID:20588296
      supporting_text: ESCRT-I and ESCRT-II direct membrane budding away from the cytosol
    - &id030
      reference_id: PMID:32424346
      supporting_text: A helical assembly of human ESCRT-I scaffolds reverse-topology membrane scission
    - &id031
      reference_id: PMID:32424346
      supporting_text: ESCRT-I is not merely a bridging adaptor
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:18005716
  qualifier: enables
  review:
    summary: Protein binding is too generic to represent VPS37B function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal 
      sorting/membrane remodeling, not generic protein binding from interaction assays.
    proposed_replacement_terms: *id017
    additional_reference_ids:
    - PMID:15218037
    - PMID:18005716
    - PMID:21757351
    - PMID:22405001
    - PMID:32424346
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    - PMID:15218037
    - PMID:21757351
    - PMID:22405001
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    supported_by:
    - *id005
    - *id006
    - *id007
    - *id008
    - *id009
    - *id010
    - *id011
    - *id012
    - *id013
    - *id014
    - *id015
    - *id016
- term:
    id: GO:0016236
    label: macroautophagy
  evidence_type: TAS
  original_reference_id: PMID:20588296
  qualifier: involved_in
  review:
    summary: Macroautophagy is over-annotated for VPS37B.
    action: MARK_AS_OVER_ANNOTATED
    reason: The accessible ESCRT autophagy evidence is broad or VPS37A-specific; there is not enough direct 
      evidence to make VPS37B a phagophore-closure/autophagosome assembly factor.
    proposed_replacement_terms:
    - id: GO:0000813
      label: ESCRT I complex
    - id: GO:0090148
      label: membrane fission
    additional_reference_ids: *id020
    supported_by: *id021
- term:
    id: GO:0000813
    label: ESCRT I complex
  evidence_type: TAS
  original_reference_id: PMID:20588296
  qualifier: part_of
  review:
    summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation.
    action: ACCEPT
    reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes.
    additional_reference_ids: *id003
    supported_by: *id004
- term:
    id: GO:0036258
    label: multivesicular body assembly
  evidence_type: TAS
  original_reference_id: PMID:20588296
  qualifier: involved_in
  review:
    summary: multivesicular body assembly is supported as part of the core VPS37B/ESCRT-I endosomal 
      cargo-sorting role.
    action: ACCEPT
    reason: VPS37B/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting and MVB biogenesis.
    additional_reference_ids: *id001
    supported_by: *id002
- term:
    id: GO:0039702
    label: viral budding via host ESCRT complex
  evidence_type: TAS
  original_reference_id: PMID:20588296
  qualifier: involved_in
  review:
    summary: viral budding via host ESCRT complex is supported as a secondary context but is not the core 
      VPS37B proteostasis function.
    action: KEEP_AS_NON_CORE
    reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect 
      ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role.
    additional_reference_ids: *id022
    supported_by: *id023
- term:
    id: GO:0000813
    label: ESCRT I complex
  evidence_type: IDA
  original_reference_id: PMID:18005716
  qualifier: part_of
  review:
    summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation.
    action: ACCEPT
    reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes.
    additional_reference_ids: *id003
    supported_by: *id004
- term:
    id: GO:0005768
    label: endosome
  evidence_type: IDA
  original_reference_id: PMID:17940959
  qualifier: located_in
  review:
    summary: endosome localization is supported and relevant to VPS37B/ESCRT-I function.
    action: ACCEPT
    reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal 
      membranes in the VPS4-dependent ESCRT cycle.
    additional_reference_ids: *id018
    supported_by: *id019
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: IDA
  original_reference_id: PMID:17940959
  qualifier: located_in
  review:
    summary: plasma membrane is supported as a secondary context but is not the core VPS37B proteostasis 
      function.
    action: KEEP_AS_NON_CORE
    reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect 
      ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role.
    additional_reference_ids: *id022
    supported_by: *id023
- term:
    id: GO:1903774
    label: positive regulation of viral budding via host ESCRT complex
  evidence_type: IMP
  original_reference_id: PMID:15218037
  qualifier: involved_in
  review:
    summary: positive regulation of viral budding via host ESCRT complex is supported as a secondary context 
      but is not the core VPS37B proteostasis function.
    action: KEEP_AS_NON_CORE
    reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect 
      ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role.
    additional_reference_ids: *id022
    supported_by: *id023
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:15218037
  qualifier: enables
  review:
    summary: Protein binding is too generic to represent VPS37B function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal 
      sorting/membrane remodeling, not generic protein binding from interaction assays.
    proposed_replacement_terms: *id017
    additional_reference_ids:
    - PMID:15218037
    - PMID:18005716
    - PMID:21757351
    - PMID:22405001
    - PMID:32424346
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    - PMID:15218037
    - PMID:21757351
    - PMID:22405001
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    supported_by:
    - *id005
    - *id006
    - *id007
    - *id008
    - *id009
    - *id010
    - *id011
    - *id012
    - *id013
    - *id014
    - *id015
    - *id016
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IDA
  original_reference_id: PMID:15218037
  qualifier: located_in
  review:
    summary: cytoplasm is supported as a secondary context but is not the core VPS37B proteostasis function.
    action: KEEP_AS_NON_CORE
    reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect 
      ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role.
    additional_reference_ids: *id022
    supported_by: *id023
- term:
    id: GO:0010008
    label: endosome membrane
  evidence_type: IDA
  original_reference_id: PMID:15218037
  qualifier: located_in
  review:
    summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function.
    action: ACCEPT
    reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal 
      membranes in the VPS4-dependent ESCRT cycle.
    additional_reference_ids: *id018
    supported_by: *id019
- term:
    id: GO:0019076
    label: viral release from host cell
  evidence_type: IMP
  original_reference_id: PMID:15218037
  qualifier: involved_in
  review:
    summary: viral release from host cell is supported as a secondary context but is not the core VPS37B 
      proteostasis function.
    action: KEEP_AS_NON_CORE
    reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect 
      ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role.
    additional_reference_ids: *id022
    supported_by: *id023
- term:
    id: GO:0048306
    label: calcium-dependent protein binding
  evidence_type: IPI
  original_reference_id: PMID:23924735
  qualifier: enables
  review:
    summary: Calcium-dependent protein binding is supported but secondary.
    action: KEEP_AS_NON_CORE
    reason: VPS37B participates in an ALG-2/ALIX/ESCRT-I calcium-dependent ternary complex, but this is not 
      the core VPS37B proteostasis function.
    additional_reference_ids: *id024
    supported_by: *id025
- term:
    id: GO:0070062
    label: extracellular exosome
  evidence_type: HDA
  original_reference_id: PMID:23533145
  qualifier: located_in
  review:
    summary: extracellular exosome is supported as a secondary context but is not the core VPS37B proteostasis
      function.
    action: KEEP_AS_NON_CORE
    reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect 
      ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role.
    additional_reference_ids: *id022
    supported_by: *id023
- term:
    id: GO:0000813
    label: ESCRT I complex
  evidence_type: IDA
  original_reference_id: PMID:22232651
  qualifier: part_of
  review:
    summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation.
    action: ACCEPT
    reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes.
    additional_reference_ids: *id003
    supported_by: *id004
- term:
    id: GO:0070062
    label: extracellular exosome
  evidence_type: HDA
  original_reference_id: PMID:19056867
  qualifier: located_in
  review:
    summary: extracellular exosome is supported as a secondary context but is not the core VPS37B proteostasis
      function.
    action: KEEP_AS_NON_CORE
    reason: Viral budding/release, exosome, plasma membrane, and broad cytoplasmic contexts use or detect 
      ESCRT machinery but are secondary to the core endosomal ESCRT-I sorting role.
    additional_reference_ids: *id022
    supported_by: *id023
- term:
    id: GO:0000813
    label: ESCRT I complex
  evidence_type: IDA
  original_reference_id: PMID:22405001
  qualifier: part_of
  review:
    summary: ESCRT-I complex membership is the central VPS37B cellular-component annotation.
    action: ACCEPT
    reason: VPS37B is a VPS37-family ESCRT-I subunit in TSG101-VPS28-VPS37-MVB12/UBAP variant complexes.
    additional_reference_ids: *id003
    supported_by: *id004
- term:
    id: GO:0010008
    label: endosome membrane
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-184269
  qualifier: located_in
  review:
    summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function.
    action: ACCEPT
    reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal 
      membranes in the VPS4-dependent ESCRT cycle.
    additional_reference_ids: *id018
    supported_by: *id019
- term:
    id: GO:0010008
    label: endosome membrane
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-3149434
  qualifier: located_in
  review:
    summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function.
    action: ACCEPT
    reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal 
      membranes in the VPS4-dependent ESCRT cycle.
    additional_reference_ids: *id018
    supported_by: *id019
- term:
    id: GO:0010008
    label: endosome membrane
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-3159232
  qualifier: located_in
  review:
    summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function.
    action: ACCEPT
    reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal 
      membranes in the VPS4-dependent ESCRT cycle.
    additional_reference_ids: *id018
    supported_by: *id019
- term:
    id: GO:0010008
    label: endosome membrane
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-917696
  qualifier: located_in
  review:
    summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function.
    action: ACCEPT
    reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal 
      membranes in the VPS4-dependent ESCRT cycle.
    additional_reference_ids: *id018
    supported_by: *id019
- term:
    id: GO:0010008
    label: endosome membrane
  evidence_type: TAS
  original_reference_id: Reactome:R-HSA-917730
  qualifier: located_in
  review:
    summary: endosome membrane localization is supported and relevant to VPS37B/ESCRT-I function.
    action: ACCEPT
    reason: VPS37B is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to endosomal 
      membranes in the VPS4-dependent ESCRT cycle.
    additional_reference_ids: *id018
    supported_by: *id019
- term:
    id: GO:0030496
    label: midbody
  evidence_type: IDA
  original_reference_id: PMID:19129479
  qualifier: located_in
  review:
    summary: Midbody localization is a secondary ESCRT/cytokinesis context and is not core for VPS37B.
    action: KEEP_AS_NON_CORE
    reason: ESCRT machinery functions in cytokinesis, and VPS37B interacts with IST1, but the core 
      proteostasis review focus is endosomal ESCRT-I sorting and membrane remodeling.
    additional_reference_ids: *id022
    supported_by: *id023
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:19129479
  qualifier: enables
  review:
    summary: Protein binding is too generic to represent VPS37B function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The informative function is ESCRT-I complex membership and ESCRT-I-mediated endosomal 
      sorting/membrane remodeling, not generic protein binding from interaction assays.
    proposed_replacement_terms: *id017
    additional_reference_ids:
    - PMID:15218037
    - PMID:18005716
    - PMID:21757351
    - PMID:22405001
    - PMID:32424346
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    - PMID:15218037
    - PMID:21757351
    - PMID:22405001
    - file:human/VPS37B/VPS37B-uniprot.txt
    - file:human/VPS37B/VPS37B-notes.md
    supported_by:
    - *id005
    - *id006
    - *id007
    - *id008
    - *id009
    - *id010
    - *id011
    - *id012
    - *id013
    - *id014
    - *id015
    - *id016
references:
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
- id: GO_REF:0000044
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, 
    accompanied by conservative changes to GO terms applied by UniProt
  findings: []
- id: GO_REF:0000117
  title: Electronic Gene Ontology annotations created by ARBA machine learning models
  findings: []
- id: PMID:15218037
  title: The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding.
  findings: []
- id: PMID:17940959
  title: Involvement of vacuolar protein sorting pathway in Ebola virus release independent of TSG101 
    interaction.
  findings: []
- id: PMID:18005716
  title: Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding.
  findings: []
- id: PMID:19056867
  title: Large-scale proteomics and phosphoproteomics of urinary exosomes.
  findings: []
- id: PMID:19129479
  title: Biochemical analyses of human IST1 and its function in cytokinesis.
  findings: []
- id: PMID:20588296
  title: "Membrane budding and scission by the ESCRT machinery: it's all in the neck."
  findings: []
- id: PMID:21516116
  title: Next-generation sequencing to generate interactome datasets.
  findings: []
- id: PMID:21757351
  title: UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.
  findings: []
- id: PMID:22232651
  title: Structural basis for membrane targeting by the MVB12-associated β-prism domain of the human ESCRT-I 
    MVB12 subunit.
  findings: []
- id: PMID:22405001
  title: The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain.
  findings: []
- id: PMID:23533145
  title: In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
  findings: []
- id: PMID:23924735
  title: VPS37 isoforms differentially modulate the ternary complex formation of ALIX, ALG-2, and ESCRT-I.
  findings: []
- id: PMID:25416956
  title: A proteome-scale map of the human interactome network.
  findings: []
- id: PMID:26871637
  title: Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
  findings: []
- id: PMID:28514442
  title: Architecture of the human interactome defines protein communities and disease networks.
  findings: []
- id: PMID:31515488
  title: Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum
    in human populations.
  findings: []
- id: PMID:32296183
  title: A reference map of the human binary protein interactome.
  findings: []
- id: PMID:32424346
  title: A helical assembly of human ESCRT-I scaffolds reverse-topology membrane scission.
  findings: []
- id: PMID:33961781
  title: Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
  findings: []
- id: PMID:35271311
  title: 'OpenCell: Endogenous tagging for the cartography of human cellular organization.'
  findings: []
- id: Reactome:R-HSA-184269
  title: Monoubiquitinated N-myristoyl GAG polyprotein is targeted to the late endosomal vesicle membrane by 
    the ESCRT-I complex
  findings: []
- id: Reactome:R-HSA-3149434
  title: Transport of GAG to the Plasma Membrane
  findings: []
- id: Reactome:R-HSA-3159232
  title: Recruitment Of HIV Virion Budding Machinery
  findings: []
- id: Reactome:R-HSA-917696
  title: Cargo Sequestration
  findings: []
- id: Reactome:R-HSA-917730
  title: Cargo Recognition And Sorting
  findings: []
- id: PMID:31519728
  title: VPS37A directs ESCRT recruitment for phagophore closure.
  findings: []
- id: file:human/VPS37B/VPS37B-uniprot.txt
  title: UniProtKB record for human VPS37B
  findings: []
- id: file:human/VPS37B/VPS37B-notes.md
  title: VPS37B review notes
  findings: []
core_functions:
- description: VPS37B is a structural ESCRT-I subunit that helps organize the TSG101-VPS28-VPS37-MVB12/UBAP 
    complex for ubiquitin-dependent endosomal cargo sorting, MVB assembly, and ESCRT-I-linked reverse-topology
    membrane remodeling.
  directly_involved_in:
  - id: GO:0043162
    label: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
  - id: GO:0036258
    label: multivesicular body assembly
  - id: GO:0090148
    label: membrane fission
  locations:
  - id: GO:0010008
    label: endosome membrane
  - id: GO:0031902
    label: late endosome membrane
  - id: GO:0005768
    label: endosome
  in_complex:
    id: GO:0000813
    label: ESCRT I complex
  supported_by:
  - *id005
  - *id006
  - *id007
  - *id008
  - *id009
  - *id010
  - *id011
  - *id012
  - *id013
  - *id014
  - *id015
  - *id016
  - *id026
  - *id027
  - *id028
  - *id029
  - *id030
  - *id031
proposed_new_terms: []
suggested_questions:
- question: Should VPS37B macroautophagy annotations be retired or kept non-core given that the direct 
    phagophore-closure evidence is VPS37A-specific?
  experts:
  - GO autophagy editors
  - GO ESCRT curators
- question: Should generic VPS37B protein-binding annotations be replaced by ESCRT-I complex membership and 
    specific endosomal sorting terms?
  experts:
  - GO molecular function editors
  - UniProt curators
suggested_experiments:
- experiment_type: VPS37 paralog phagophore closure comparison
  hypothesis: VPS37A, but not VPS37B, is the VPS37 paralog specialized for phagophore closure because VPS37B 
    lacks the VPS37A PUEV-dependent targeting function.
  description: Compare VPS37A, VPS37B, and VPS37C depletion/rescue in HT-LC3 autophagosome closure assays and 
    parallel EGFR MVB-sorting assays, using endogenous expression or matched rescue levels.

VPS37B

Gene Description

Existing Annotations Review

Core Functions

VPS37B is a structural ESCRT-I subunit that helps organize the TSG101-VPS28-VPS37-MVB12/UBAP complex for ubiquitin-dependent endosomal cargo sorting, MVB assembly, and ESCRT-I-linked reverse-topology membrane remodeling.

References

Suggested Questions for Experts

Suggested Experiments

📚 Additional Documentation

Notes

VPS37B review notes

Scope

Evidence synthesis

Falcon

Pn Notes

VPS37B PN Consistency Notes

Source Files Checked

Deep Research Files

AIGR Review Snapshot

PN Consistency Summary

Full Consistency Review

PN Dossier Context

PN row 1: Autophagy-Lysosome Pathway | Autophagosome closure maturation and lysosome fusion | Sealing of autophagophore membrane | ESCRT-I complex component

Projected GO annotations (2)

Note

📄 View Raw YAML