id: A5D8V6 gene_symbol: VPS37C product_type: PROTEIN status: COMPLETE taxon: id: NCBITaxon:9606 label: Homo sapiens description: VPS37C is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and MVB12/UBAP-family partners. The best-supported cellular function is ESCRT-I-dependent endosomal sorting of ubiquitinated cargo into multivesicular bodies, with late-endosome/endosome-membrane localization and a secondary viral budding context. Current local evidence does not support transferring the VPS37A-specific phagophore-closure role to VPS37C as a core annotation. existing_annotations: - term: id: GO:0043162 label: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: involved_in review: summary: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway is supported as part of the core VPS37C/ESCRT-I endosomal cargo-sorting role. action: ACCEPT reason: VPS37C/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting into multivesicular bodies. additional_reference_ids: &id001 - PMID:15509564 - PMID:18005716 - file:human/VPS37C/VPS37C-uniprot.txt - file:human/VPS37C/VPS37C-notes.md supported_by: &id002 - &id028 reference_id: file:human/VPS37C/VPS37C-uniprot.txt supporting_text: Required for the sorting of endocytic - &id029 reference_id: file:human/VPS37C/VPS37C-uniprot.txt supporting_text: ubiquitinated cargos into multivesicular bodies - &id030 reference_id: PMID:15509564 supporting_text: required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies - &id031 reference_id: PMID:15509564 supporting_text: binds to another class E VPS factor, namely Hrs - &id032 reference_id: PMID:18005716 supporting_text: plays essential roles in HIV budding and endosomal protein sorting - term: id: GO:0000813 label: ESCRT I complex evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: part_of review: summary: ESCRT-I complex membership is a central VPS37C cellular-component annotation. action: ACCEPT reason: VPS37C is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and fourth-subunit partners. additional_reference_ids: &id003 - PMID:15509564 - PMID:18005716 - PMID:22405001 - file:human/VPS37C/VPS37C-uniprot.txt - file:human/VPS37C/VPS37C-notes.md supported_by: &id004 - &id008 reference_id: file:human/VPS37C/VPS37C-uniprot.txt supporting_text: Component of the ESCRT-I complex - &id009 reference_id: file:human/VPS37C/VPS37C-uniprot.txt supporting_text: which consists of TSG101, VPS28, a VPS37 - &id010 reference_id: file:human/VPS37C/VPS37C-uniprot.txt supporting_text: Interacts with TSG101, VPS28, MVB12A and MVB12B - &id011 reference_id: PMID:15509564 supporting_text: VPS37C can form a ternary complex with Tsg101 and VPS28 - &id012 reference_id: PMID:15509564 supporting_text: Thus, this study identifies VPS37C as a functional component of mammalian ESCRT-I - &id026 reference_id: PMID:18005716 supporting_text: All ESCRT-I complexes contain three common subunits (TSG101, VPS28, and VPS37) - &id027 reference_id: PMID:22405001 supporting_text: complex with Vps23/TSG101, VPS28, and VPS37 - term: id: GO:0006612 label: protein targeting to membrane evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: involved_in review: summary: protein targeting to membrane is too broad for VPS37C ESCRT-I function. action: MODIFY reason: The supported process is ESCRT-I-dependent endosomal cargo sorting into the MVB pathway, not generic protein targeting. proposed_replacement_terms: - id: GO:0043162 label: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway - id: GO:0036258 label: multivesicular body assembly additional_reference_ids: *id001 supported_by: *id002 - term: id: GO:0006623 label: protein targeting to vacuole evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: involved_in review: summary: protein targeting to vacuole is too broad for VPS37C ESCRT-I function. action: MODIFY reason: The supported process is ESCRT-I-dependent endosomal cargo sorting into the MVB pathway, not generic protein targeting. proposed_replacement_terms: - id: GO:0043162 label: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway - id: GO:0036258 label: multivesicular body assembly additional_reference_ids: *id001 supported_by: *id002 - term: id: GO:0000813 label: ESCRT I complex evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: part_of review: summary: ESCRT-I complex membership is a central VPS37C cellular-component annotation. action: ACCEPT reason: VPS37C is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and fourth-subunit partners. additional_reference_ids: *id003 supported_by: *id004 - term: id: GO:0016236 label: macroautophagy evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in review: summary: Macroautophagy is over-annotated for VPS37C. action: MARK_AS_OVER_ANNOTATED reason: The accessible ESCRT autophagy evidence is broad or VPS37A-specific; there is not enough direct evidence to make VPS37C a phagophore-closure/autophagosome assembly factor. proposed_replacement_terms: - id: GO:0000813 label: ESCRT I complex additional_reference_ids: &id016 - PMID:20588296 - PMID:31519728 - PMID:32424346 - file:human/VPS37C/VPS37C-notes.md supported_by: &id017 - reference_id: PMID:20588296 supporting_text: viral budding, cytokinesis and, probably, autophagy - reference_id: PMID:20588296 supporting_text: direct neck closure reaction in autophagy - reference_id: PMID:31519728 supporting_text: identify the ESCRT-I subunit VPS37A as a critical component - reference_id: PMID:31519728 supporting_text: required for autophagosome completion - term: id: GO:0031902 label: late endosome membrane evidence_type: IEA original_reference_id: GO_REF:0000044 qualifier: located_in review: summary: late endosome membrane localization is supported and relevant to VPS37C/ESCRT-I function. action: ACCEPT reason: VPS37C is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to aberrant endosomes in ESCRT perturbation assays. additional_reference_ids: &id024 - PMID:15509564 - file:human/VPS37C/VPS37C-uniprot.txt - file:human/VPS37C/VPS37C-notes.md supported_by: &id025 - &id013 reference_id: file:human/VPS37C/VPS37C-uniprot.txt supporting_text: Late endosome membrane - &id014 reference_id: file:human/VPS37C/VPS37C-uniprot.txt supporting_text: Probably associates with membranes - &id015 reference_id: PMID:15509564 supporting_text: VPS37C is recruited to aberrant endosomes - term: id: GO:0036258 label: multivesicular body assembly evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in review: summary: multivesicular body assembly is supported as part of the core VPS37C/ESCRT-I endosomal cargo-sorting role. action: ACCEPT reason: VPS37C/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting into multivesicular bodies. additional_reference_ids: *id001 supported_by: *id002 - term: id: GO:0039702 label: viral budding via host ESCRT complex evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: involved_in review: summary: Viral budding via host ESCRT complex is supported as a secondary context but is not the core VPS37C proteostasis function. action: KEEP_AS_NON_CORE reason: VPS37C can support ESCRT-I-dependent viral budding, but the core cellular role is endosomal ESCRT-I cargo sorting. additional_reference_ids: &id018 - PMID:15509564 - PMID:18005716 - PMID:20588296 - file:human/VPS37C/VPS37C-uniprot.txt - file:human/VPS37C/VPS37C-notes.md supported_by: &id019 - reference_id: PMID:15509564 supporting_text: VPS37C is recruited to the plasma membrane - reference_id: PMID:15509564 supporting_text: direct fusion of VPS37C to HIV-1 Gag - reference_id: PMID:15509564 supporting_text: inhibited by VPS37C depletion - reference_id: PMID:18005716 supporting_text: plays essential roles in HIV budding and endosomal protein sorting - reference_id: PMID:20588296 supporting_text: viral budding, cytokinesis and, probably, autophagy - term: id: GO:0048306 label: calcium-dependent protein binding evidence_type: IEA original_reference_id: GO_REF:0000117 qualifier: enables review: summary: Calcium-dependent protein binding is supported but secondary. action: KEEP_AS_NON_CORE reason: VPS37C participates in an ALG-2/ALIX/ESCRT-I calcium-dependent ternary-complex context, but this is not the core VPS37C proteostasis function. additional_reference_ids: &id020 - PMID:23924735 - file:human/VPS37C/VPS37C-notes.md supported_by: &id021 - reference_id: PMID:23924735 supporting_text: VPS37B and VPS37C appeared to interact with ALG-2 - reference_id: PMID:23924735 supporting_text: adaptor protein that bridges ALIX and ESCRT-I - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:17853893 qualifier: enables review: summary: Protein binding is too generic to represent VPS37C function. action: MARK_AS_OVER_ANNOTATED reason: The informative annotations are ESCRT-I complex membership, endosomal MVB sorting, and specific ESCRT-I interaction contexts, not generic protein binding from interaction assays or screens. proposed_replacement_terms: &id005 - id: GO:0000813 label: ESCRT I complex additional_reference_ids: &id006 - PMID:15509564 - PMID:17853893 - PMID:25416956 - PMID:31515488 - PMID:32296183 - PMID:35271311 - file:human/VPS37C/VPS37C-notes.md supported_by: &id007 - reference_id: PMID:15509564 supporting_text: VPS37C can form a ternary complex with Tsg101 and VPS28 - reference_id: file:human/VPS37C/VPS37C-notes.md supporting_text: Generic `protein binding` rows are over-annotated - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:25416956 qualifier: enables review: summary: Protein binding is too generic to represent VPS37C function. action: MARK_AS_OVER_ANNOTATED reason: The informative annotations are ESCRT-I complex membership, endosomal MVB sorting, and specific ESCRT-I interaction contexts, not generic protein binding from interaction assays or screens. proposed_replacement_terms: *id005 additional_reference_ids: *id006 supported_by: *id007 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:31515488 qualifier: enables review: summary: Protein binding is too generic to represent VPS37C function. action: MARK_AS_OVER_ANNOTATED reason: The informative annotations are ESCRT-I complex membership, endosomal MVB sorting, and specific ESCRT-I interaction contexts, not generic protein binding from interaction assays or screens. proposed_replacement_terms: *id005 additional_reference_ids: *id006 supported_by: *id007 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:32296183 qualifier: enables review: summary: Protein binding is too generic to represent VPS37C function. action: MARK_AS_OVER_ANNOTATED reason: The informative annotations are ESCRT-I complex membership, endosomal MVB sorting, and specific ESCRT-I interaction contexts, not generic protein binding from interaction assays or screens. proposed_replacement_terms: *id005 additional_reference_ids: *id006 supported_by: *id007 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:35271311 qualifier: enables review: summary: Protein binding is too generic to represent VPS37C function. action: MARK_AS_OVER_ANNOTATED reason: The informative annotations are ESCRT-I complex membership, endosomal MVB sorting, and specific ESCRT-I interaction contexts, not generic protein binding from interaction assays or screens. proposed_replacement_terms: *id005 additional_reference_ids: *id006 supported_by: *id007 - term: id: GO:0005654 label: nucleoplasm evidence_type: IDA original_reference_id: GO_REF:0000052 qualifier: located_in review: summary: Nucleoplasm localization is not part of the core VPS37C ESCRT-I function. action: KEEP_AS_NON_CORE reason: The core supported localization is late endosome/endosome membrane; the nucleoplasm row comes from high-throughput localization mapping and should not drive the functional model. additional_reference_ids: - file:human/VPS37C/VPS37C-notes.md supported_by: - reference_id: file:human/VPS37C/VPS37C-notes.md supporting_text: Extracellular exosome and nucleoplasm rows come from high-throughput localization/proteomics and should remain non-core - term: id: GO:0000813 label: ESCRT I complex evidence_type: IPI original_reference_id: PMID:18005716 qualifier: part_of review: summary: ESCRT-I complex membership is a central VPS37C cellular-component annotation. action: ACCEPT reason: VPS37C is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and fourth-subunit partners. additional_reference_ids: *id003 supported_by: *id004 - term: id: GO:0000813 label: ESCRT I complex evidence_type: IPI original_reference_id: PMID:21757351 qualifier: part_of review: summary: ESCRT-I complex membership is correct for VPS37C, but PMID:21757351 is not the strongest VPS37C-specific support. action: ACCEPT reason: The term is correct from VPS37C-specific and shared ESCRT-I evidence; however, PMID:21757351 specifically defines a UBAP1/VPS37A endosome-specific complex and says it does not contain VPS37C. additional_reference_ids: - PMID:15509564 - PMID:18005716 - PMID:21757351 - file:human/VPS37C/VPS37C-uniprot.txt - file:human/VPS37C/VPS37C-notes.md supported_by: - *id008 - *id009 - *id010 - *id011 - *id012 - reference_id: PMID:21757351 supporting_text: contains VPS37A but not VPS37C - term: id: GO:0010008 label: endosome membrane evidence_type: NAS original_reference_id: PMID:32424346 qualifier: located_in review: summary: endosome membrane is correct for VPS37C, but PMID:32424346 is indirect for this isoform. action: ACCEPT reason: Endosome-membrane localization is supported by VPS37C-specific evidence; PMID:32424346 supports general ESCRT-I mechanism using a VPS37B-containing headpiece. additional_reference_ids: - PMID:15509564 - PMID:32424346 - file:human/VPS37C/VPS37C-uniprot.txt - file:human/VPS37C/VPS37C-notes.md supported_by: - *id013 - *id014 - *id015 - reference_id: PMID:32424346 supporting_text: comprising TSG101-VPS28-VPS37B-MVB12A - term: id: GO:0036258 label: multivesicular body assembly evidence_type: NAS original_reference_id: PMID:32424346 qualifier: involved_in review: summary: multivesicular body assembly is supported as part of the core VPS37C/ESCRT-I endosomal cargo-sorting role. action: ACCEPT reason: VPS37C/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting into multivesicular bodies. additional_reference_ids: *id001 supported_by: *id002 - term: id: GO:0043328 label: protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway evidence_type: NAS original_reference_id: PMID:32424346 qualifier: involved_in review: summary: protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway is supported as part of the core VPS37C/ESCRT-I endosomal cargo-sorting role. action: ACCEPT reason: VPS37C/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting into multivesicular bodies. additional_reference_ids: *id001 supported_by: *id002 - term: id: GO:0090148 label: membrane fission evidence_type: NAS original_reference_id: PMID:32424346 qualifier: involved_in review: summary: Membrane fission is a broad ESCRT-I mechanism annotation and is over-transferred for VPS37C as written. action: MARK_AS_OVER_ANNOTATED reason: The cited structural/autophagy work directly tested a VPS37B-containing headpiece and VPS28 interface mutants, not VPS37C; the safer VPS37C annotations are ESCRT-I complex and MVB sorting. proposed_replacement_terms: - id: GO:0000813 label: ESCRT I complex - id: GO:0036258 label: multivesicular body assembly additional_reference_ids: - PMID:32424346 - PMID:15509564 - file:human/VPS37C/VPS37C-notes.md supported_by: - reference_id: PMID:32424346 supporting_text: comprising TSG101-VPS28-VPS37B-MVB12A - reference_id: PMID:32424346 supporting_text: ESCRT-I is not merely a bridging adaptor - reference_id: PMID:15509564 supporting_text: functional component of mammalian ESCRT-I - term: id: GO:0016236 label: macroautophagy evidence_type: TAS original_reference_id: PMID:20588296 qualifier: involved_in review: summary: Macroautophagy is over-annotated for VPS37C. action: MARK_AS_OVER_ANNOTATED reason: The accessible ESCRT autophagy evidence is broad or VPS37A-specific; there is not enough direct evidence to make VPS37C a phagophore-closure/autophagosome assembly factor. proposed_replacement_terms: - id: GO:0000813 label: ESCRT I complex additional_reference_ids: *id016 supported_by: *id017 - term: id: GO:0000813 label: ESCRT I complex evidence_type: TAS original_reference_id: PMID:20588296 qualifier: part_of review: summary: ESCRT-I complex membership is a central VPS37C cellular-component annotation. action: ACCEPT reason: VPS37C is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and fourth-subunit partners. additional_reference_ids: *id003 supported_by: *id004 - term: id: GO:0036258 label: multivesicular body assembly evidence_type: TAS original_reference_id: PMID:20588296 qualifier: involved_in review: summary: multivesicular body assembly is supported as part of the core VPS37C/ESCRT-I endosomal cargo-sorting role. action: ACCEPT reason: VPS37C/ESCRT-I supports ubiquitin-dependent endosomal cargo sorting into multivesicular bodies. additional_reference_ids: *id001 supported_by: *id002 - term: id: GO:0039702 label: viral budding via host ESCRT complex evidence_type: TAS original_reference_id: PMID:20588296 qualifier: involved_in review: summary: Viral budding via host ESCRT complex is supported as a secondary context but is not the core VPS37C proteostasis function. action: KEEP_AS_NON_CORE reason: VPS37C can support ESCRT-I-dependent viral budding, but the core cellular role is endosomal ESCRT-I cargo sorting. additional_reference_ids: *id018 supported_by: *id019 - term: id: GO:0000813 label: ESCRT I complex evidence_type: IDA original_reference_id: PMID:18005716 qualifier: part_of review: summary: ESCRT-I complex membership is a central VPS37C cellular-component annotation. action: ACCEPT reason: VPS37C is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and fourth-subunit partners. additional_reference_ids: *id003 supported_by: *id004 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:23924735 qualifier: enables review: summary: Protein binding is too generic to represent VPS37C function. action: MARK_AS_OVER_ANNOTATED reason: The informative annotations are ESCRT-I complex membership, endosomal MVB sorting, and specific ESCRT-I interaction contexts, not generic protein binding from interaction assays or screens. proposed_replacement_terms: - id: GO:0048306 label: calcium-dependent protein binding additional_reference_ids: *id020 supported_by: *id021 - term: id: GO:0048306 label: calcium-dependent protein binding evidence_type: IPI original_reference_id: PMID:23924735 qualifier: enables review: summary: Calcium-dependent protein binding is supported but secondary. action: KEEP_AS_NON_CORE reason: VPS37C participates in an ALG-2/ALIX/ESCRT-I calcium-dependent ternary-complex context, but this is not the core VPS37C proteostasis function. additional_reference_ids: *id020 supported_by: *id021 - term: id: GO:0070062 label: extracellular exosome evidence_type: HDA original_reference_id: PMID:23533145 qualifier: located_in review: summary: Extracellular exosome is a high-throughput proteomics/localization context and not a core VPS37C annotation. action: KEEP_AS_NON_CORE reason: Exosome detection is not the central VPS37C ESCRT-I endosomal sorting function. additional_reference_ids: &id022 - PMID:19056867 - PMID:23533145 - file:human/VPS37C/VPS37C-notes.md supported_by: &id023 - reference_id: PMID:19056867 supporting_text: Large-scale proteomics and phosphoproteomics of urinary exosomes - reference_id: PMID:23533145 supporting_text: In-depth proteomic analyses of exosomes - term: id: GO:0070062 label: extracellular exosome evidence_type: HDA original_reference_id: PMID:19056867 qualifier: located_in review: summary: Extracellular exosome is a high-throughput proteomics/localization context and not a core VPS37C annotation. action: KEEP_AS_NON_CORE reason: Exosome detection is not the central VPS37C ESCRT-I endosomal sorting function. additional_reference_ids: *id022 supported_by: *id023 - term: id: GO:0000813 label: ESCRT I complex evidence_type: IDA original_reference_id: PMID:22405001 qualifier: part_of review: summary: ESCRT-I complex membership is a central VPS37C cellular-component annotation. action: ACCEPT reason: VPS37C is a VPS37-family ESCRT-I subunit that complexes with TSG101, VPS28, and fourth-subunit partners. additional_reference_ids: *id003 supported_by: *id004 - term: id: GO:0010008 label: endosome membrane evidence_type: TAS original_reference_id: Reactome:R-HSA-184269 qualifier: located_in review: summary: endosome membrane localization is supported and relevant to VPS37C/ESCRT-I function. action: ACCEPT reason: VPS37C is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to aberrant endosomes in ESCRT perturbation assays. additional_reference_ids: *id024 supported_by: *id025 - term: id: GO:0010008 label: endosome membrane evidence_type: TAS original_reference_id: Reactome:R-HSA-3149434 qualifier: located_in review: summary: endosome membrane localization is supported and relevant to VPS37C/ESCRT-I function. action: ACCEPT reason: VPS37C is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to aberrant endosomes in ESCRT perturbation assays. additional_reference_ids: *id024 supported_by: *id025 - term: id: GO:0010008 label: endosome membrane evidence_type: TAS original_reference_id: Reactome:R-HSA-3159232 qualifier: located_in review: summary: endosome membrane localization is supported and relevant to VPS37C/ESCRT-I function. action: ACCEPT reason: VPS37C is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to aberrant endosomes in ESCRT perturbation assays. additional_reference_ids: *id024 supported_by: *id025 - term: id: GO:0010008 label: endosome membrane evidence_type: TAS original_reference_id: Reactome:R-HSA-917696 qualifier: located_in review: summary: endosome membrane localization is supported and relevant to VPS37C/ESCRT-I function. action: ACCEPT reason: VPS37C is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to aberrant endosomes in ESCRT perturbation assays. additional_reference_ids: *id024 supported_by: *id025 - term: id: GO:0010008 label: endosome membrane evidence_type: TAS original_reference_id: Reactome:R-HSA-917730 qualifier: located_in review: summary: endosome membrane localization is supported and relevant to VPS37C/ESCRT-I function. action: ACCEPT reason: VPS37C is a peripheral late-endosome/endosome-membrane ESCRT-I component recruited to aberrant endosomes in ESCRT perturbation assays. additional_reference_ids: *id024 supported_by: *id025 references: - id: GO_REF:0000033 title: Annotation inferences using phylogenetic trees findings: [] - id: GO_REF:0000044 title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt findings: [] - id: GO_REF:0000052 title: Gene Ontology annotation based on curation of immunofluorescence data findings: [] - id: GO_REF:0000117 title: Electronic Gene Ontology annotations created by ARBA machine learning models findings: [] - id: PMID:17853893 title: Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis. findings: [] - id: PMID:18005716 title: Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding. findings: [] - id: PMID:19056867 title: Large-scale proteomics and phosphoproteomics of urinary exosomes. findings: [] - id: PMID:20588296 title: 'Membrane budding and scission by the ESCRT machinery: it''s all in the neck.' findings: [] - id: PMID:21757351 title: UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting. findings: [] - id: PMID:22405001 title: The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain. findings: [] - id: PMID:23533145 title: In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine. findings: [] - id: PMID:23924735 title: VPS37 isoforms differentially modulate the ternary complex formation of ALIX, ALG-2, and ESCRT-I. findings: [] - id: PMID:25416956 title: A proteome-scale map of the human interactome network. findings: [] - id: PMID:31515488 title: Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations. findings: [] - id: PMID:32296183 title: A reference map of the human binary protein interactome. findings: [] - id: PMID:32424346 title: A helical assembly of human ESCRT-I scaffolds reverse-topology membrane scission. findings: [] - id: PMID:35271311 title: 'OpenCell: Endogenous tagging for the cartography of human cellular organization.' findings: [] - id: Reactome:R-HSA-184269 title: Monoubiquitinated N-myristoyl GAG polyprotein is targeted to the late endosomal vesicle membrane by the ESCRT-I complex findings: [] - id: Reactome:R-HSA-3149434 title: Transport of GAG to the Plasma Membrane findings: [] - id: Reactome:R-HSA-3159232 title: Recruitment Of HIV Virion Budding Machinery findings: [] - id: Reactome:R-HSA-917696 title: Cargo Sequestration findings: [] - id: Reactome:R-HSA-917730 title: Cargo Recognition And Sorting findings: [] - id: PMID:15509564 title: Identification of human VPS37C, a component of endosomal sorting complex required for transport-I important for viral budding. findings: [] - id: PMID:31519728 title: VPS37A directs ESCRT recruitment for phagophore closure. findings: [] - id: file:human/VPS37C/VPS37C-uniprot.txt title: UniProtKB record for human VPS37C findings: [] - id: file:human/VPS37C/VPS37C-notes.md title: VPS37C review notes findings: [] core_functions: - description: VPS37C is a structural ESCRT-I subunit that helps organize TSG101-VPS28-VPS37-MVB12/UBAP-family complexes for ubiquitin-dependent endosomal cargo sorting and MVB assembly. directly_involved_in: - id: GO:0043162 label: ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway - id: GO:0036258 label: multivesicular body assembly locations: - id: GO:0010008 label: endosome membrane - id: GO:0031902 label: late endosome membrane in_complex: id: GO:0000813 label: ESCRT I complex supported_by: - *id008 - *id009 - *id010 - *id011 - *id012 - *id026 - *id027 - *id028 - *id029 - *id030 - *id031 - *id032 - *id013 - *id014 - *id015 proposed_new_terms: [] suggested_questions: - question: Should VPS37C macroautophagy annotations be retired or kept non-core given that the direct phagophore-closure evidence is VPS37A-specific? experts: - GO autophagy editors - GO ESCRT curators - question: Should generic VPS37C protein-binding annotations be replaced by ESCRT-I complex membership, calcium-dependent ALG-2 interaction context, and specific endosomal sorting terms? experts: - GO molecular function editors - UniProt curators suggested_experiments: - experiment_type: VPS37 paralog phagophore closure comparison hypothesis: VPS37A, but not necessarily VPS37C, is the VPS37 paralog specialized for phagophore closure. description: Compare VPS37A, VPS37B, and VPS37C depletion/rescue in HT-LC3 autophagosome closure assays and parallel EGFR or tetherin MVB-sorting assays, using endogenous expression or matched rescue levels. - experiment_type: VPS37C-specific MVB sorting assay hypothesis: VPS37C contributes to a subset of ESCRT-I cargo-sorting or viral-budding contexts distinct from the UBAP1/VPS37A endosome-specific complex. description: Use VPS37C-specific knockout-rescue with quantitative cargo degradation and HIV-1 Gag recruitment/release readouts, controlling for VPS37A and VPS37B compensation.