VTI1A encodes a VTI1-family SNARE that mediates vesicle transport through interactions with target-membrane SNAREs and promotes membrane fusion. Its best-supported core function is SNAP receptor/SNARE-complex activity in retrograde trafficking from endosomes or late endosomes to the trans-Golgi network/Golgi, with related roles in Golgi/TGN membrane traffic and selected non-conventional cell-surface trafficking routes. VTI1A also has direct support in autophagy/autophagosome context through STX13/Vti1a-associated autophagic flux, but it is not an ESCRT-III complex component.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0006891 intra-Golgi vesicle-mediated transport | IBA GO_REF:0000033 | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: intra-Golgi vesicle-mediated transport. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0006896 Golgi to vacuole transport | IBA GO_REF:0000033 | MODIFY | Summary: The yeast-derived Golgi-to-vacuole term captures a trafficking idea but is not the best human VTI1A process term. Reason: Human evidence supports endosome/late-endosome to trans-Golgi network retrograde transport rather than a literal Golgi-to-vacuole process. Proposed replacements: retrograde transport, endosome to Golgi Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 |
| GO:0016236 macroautophagy | IBA GO_REF:0000033 | ACCEPT | Summary: Directly supported autophagy/autophagosome context for VTI1A: macroautophagy. Reason: PMID:24095276 directly reports Vti1a knockdown blocking autophagic flux in the STX13/CHMP2B context and GOA includes autophagosome localization from that study. Supporting Evidence: PMID:24095276 Knockdown of syntaxin 13 (STX13) or its binding partner Vti1a in mammalian cells caused LC3-positive puncta to accumulate PMID:24095276 blocks autophagic flux |
| GO:0042147 retrograde transport, endosome to Golgi | IBA GO_REF:0000033 | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: retrograde transport, endosome to Golgi. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0048280 vesicle fusion with Golgi apparatus | IBA GO_REF:0000033 | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: vesicle fusion with Golgi apparatus. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0000149 SNARE binding | IBA GO_REF:0000033 | MODIFY | Summary: The annotation reflects SNARE-partner interactions, but SNAP receptor activity is the more informative molecular-function term for VTI1A. Reason: VTI1A is a SNARE whose core molecular function is SNAP receptor/SNARE complex activity in membrane fusion; SNARE binding alone is less precise. Proposed replacements: SNAP receptor activity Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport |
| GO:0005484 SNAP receptor activity | IBA GO_REF:0000033 | ACCEPT | Summary: Supported as the core VTI1A molecular function. Reason: VTI1A is a SNARE/SNAP receptor that forms cognate SNARE complexes for membrane fusion and vesicle transport. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0005789 endoplasmic reticulum membrane | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: endoplasmic reticulum membrane. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0012507 ER to Golgi transport vesicle membrane | IBA GO_REF:0000033 | ACCEPT | Summary: Supported location for VTI1A in non-conventional ER/Golgi or trafficking-vesicle routes. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 PMID:19138172 inhibited Kv4/KChIP1traffic to the plasma membrane UniProt:Q96AJ9 Cytoplasmic vesicle |
| GO:0031902 late endosome membrane | IBA GO_REF:0000033 | ACCEPT | Summary: Supported compartment for VTI1A SNARE-mediated trafficking: late endosome membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Cytoplasmic vesicle |
| GO:0000139 Golgi membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: Golgi membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0005484 SNAP receptor activity | IEA GO_REF:0000002 | ACCEPT | Summary: Supported as the core VTI1A molecular function. Reason: VTI1A is a SNARE/SNAP receptor that forms cognate SNARE complexes for membrane fusion and vesicle transport. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0005737 cytoplasm | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: cytoplasm. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0005794 Golgi apparatus | IEA GO_REF:0000002 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: Golgi apparatus. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0005829 cytosol | IEA GO_REF:0000108 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: cytosol. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0006886 intracellular protein transport | IEA GO_REF:0000002 | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: intracellular protein transport. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0012505 endomembrane system | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: endomembrane system. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0016020 membrane | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: membrane. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0016192 vesicle-mediated transport | IEA GO_REF:0000002 | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: vesicle-mediated transport. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0016197 endosomal transport | IEA GO_REF:0000117 | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: endosomal transport. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0031410 cytoplasmic vesicle | IEA GO_REF:0000044 | ACCEPT | Summary: Supported cytoplasmic vesicle localization for VTI1A trafficking routes. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Cytoplasmic vesicle PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0061025 membrane fusion | IEA GO_REF:0000117 | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: membrane fusion. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0098588 bounding membrane of organelle | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: bounding membrane of organelle. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0032456 endocytic recycling | IMP PMID:23677696 VAMP4 is required to maintain the ribbon structure of the Go... | MODIFY | Summary: The phenotype in the cited Golgi-ribbon paper is better described as early-endosome-to-TGN retrograde traffic than generic endocytic recycling. Reason: PMID:23677696 links Vti1a to the VAMP4/STX6/STX16 SNARE pathway for early-endosome-to-TGN retrograde trafficking and Golgi organization. Proposed replacements: retrograde transport, endosome to Golgi Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN |
| GO:0005515 protein binding | IPI PMID:24095276 Syntaxin 13, a genetic modifier of mutant CHMP2B in frontote... | MARK AS OVER ANNOTATED | Summary: The STX12/VTI1A interaction is relevant, but GO:0005515 is too generic for VTI1A molecular function. Reason: The useful curation should describe VTI1A as a SNAP receptor/SNARE component in membrane traffic and autophagy-associated trafficking, not generic protein binding. Supporting Evidence: file:human/VTI1A/VTI1A-notes.md Generic protein binding is too vague for VTI1A; the useful function is SNARE-mediated membrane traffic. PMID:24095276 Knockdown of syntaxin 13 (STX13) or its binding partner Vti1a |
| GO:0005776 autophagosome | IDA PMID:24095276 Syntaxin 13, a genetic modifier of mutant CHMP2B in frontote... | ACCEPT | Summary: Directly supported autophagy/autophagosome context for VTI1A: autophagosome. Reason: PMID:24095276 directly reports Vti1a knockdown blocking autophagic flux in the STX13/CHMP2B context and GOA includes autophagosome localization from that study. Supporting Evidence: PMID:24095276 Knockdown of syntaxin 13 (STX13) or its binding partner Vti1a in mammalian cells caused LC3-positive puncta to accumulate PMID:24095276 blocks autophagic flux |
| GO:0006914 autophagy | IMP PMID:24095276 Syntaxin 13, a genetic modifier of mutant CHMP2B in frontote... | ACCEPT | Summary: Directly supported autophagy/autophagosome context for VTI1A: autophagy. Reason: PMID:24095276 directly reports Vti1a knockdown blocking autophagic flux in the STX13/CHMP2B context and GOA includes autophagosome localization from that study. Supporting Evidence: PMID:24095276 Knockdown of syntaxin 13 (STX13) or its binding partner Vti1a in mammalian cells caused LC3-positive puncta to accumulate PMID:24095276 blocks autophagic flux |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-6811431 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: trans-Golgi network membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-6814671 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: trans-Golgi network membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-6814674 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: trans-Golgi network membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-6814676 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: trans-Golgi network membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-6814678 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: trans-Golgi network membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-6814682 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: trans-Golgi network membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-6814683 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: trans-Golgi network membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-6814684 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: trans-Golgi network membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0000139 Golgi membrane | TAS Reactome:R-HSA-6811431 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: Golgi membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0000139 Golgi membrane | TAS Reactome:R-HSA-6811433 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: Golgi membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0050882 voluntary musculoskeletal movement | IMP PMID:22958904 VAMP1 mutation causes dominant hereditary spastic ataxia in ... | REMOVE | Summary: The cited paper supports VAMP1, not VTI1A, in hereditary spastic ataxia/movement phenotypes. Reason: The cached PMID identifies VAMP1 as the responsible gene and contains no VTI1A support; this phenotype should not be propagated to VTI1A. Supporting Evidence: PMID:22958904 identifies vesicle-associated membrane protein 1 (VAMP1) |
| GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport | ISS GO_REF:0000024 | ACCEPT | Summary: Supported as a non-conventional ER/Golgi or early secretory trafficking context for VTI1A. Reason: PMID:19138172 supports a VAMP7/Vti1a route for KChIP1/Kv4 traffic distinct from conventional VSVG traffic. Supporting Evidence: PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 PMID:19138172 inhibited Kv4/KChIP1traffic to the plasma membrane UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers |
| GO:0048280 vesicle fusion with Golgi apparatus | ISS GO_REF:0000024 | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: vesicle fusion with Golgi apparatus. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0005768 endosome | ISS GO_REF:0000024 | ACCEPT | Summary: Supported compartment for VTI1A SNARE-mediated trafficking: endosome. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Cytoplasmic vesicle |
| GO:0008021 synaptic vesicle | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: synaptic vesicle. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0030136 clathrin-coated vesicle | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: clathrin-coated vesicle. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0031201 SNARE complex | ISS GO_REF:0000024 | ACCEPT | Summary: Supported as a core SNARE-complex context for VTI1A. Reason: VTI1A is part of STX10/STX16/VAMP3 and VAMP4/STX6/STX16 SNARE-complex contexts that mediate endosome-to-Golgi/TGN traffic. Supporting Evidence: PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Identified in a complex containing STX6, STX12, VAMP4 and VTI1A |
| GO:0043025 neuronal cell body | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: neuronal cell body. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0044306 neuron projection terminus | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: neuron projection terminus. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0048471 perinuclear region of cytoplasm | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: perinuclear region of cytoplasm. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0005794 Golgi apparatus | IDA PMID:19224922 Differential effects of depletion of ARL1 and ARFRP1 on memb... | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: Golgi apparatus. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0042147 retrograde transport, endosome to Golgi | IMP PMID:19224922 Differential effects of depletion of ARL1 and ARFRP1 on memb... | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: retrograde transport, endosome to Golgi. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0042147 retrograde transport, endosome to Golgi | IDA PMID:18195106 A syntaxin 10-SNARE complex distinguishes two distinct trans... | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: retrograde transport, endosome to Golgi. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0005484 SNAP receptor activity | IDA PMID:15215310 Participation of the syntaxin 5/Ykt6/GS28/GS15 SNARE complex... | ACCEPT | Summary: Accepted for VTI1A on independent SNARE evidence; the cited PMID appears to describe GS15 rather than VTI1A. Reason: VTI1A clearly has SNAP receptor activity from UniProt and other VTI1A-specific evidence, but the original GS15 PMID should be reviewed as a possible source/reference error. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:15215310 syntaxin 5, GS28, Ykt6, and GS15 exist as a unique SNARE complex |
| GO:0031201 SNARE complex | TAS PMID:15215310 Participation of the syntaxin 5/Ykt6/GS28/GS15 SNARE complex... | ACCEPT | Summary: Accepted for VTI1A on independent SNARE-complex evidence; the cited PMID appears to describe GS15 rather than VTI1A. Reason: VTI1A is supported as a SNARE-complex component by VTI1A-specific evidence, but the original GS15 PMID should be reviewed as a possible source/reference error. Supporting Evidence: PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Identified in a complex containing STX6, STX12, VAMP4 and VTI1A PMID:15215310 syntaxin 5, GS28, Ykt6, and GS15 exist as a unique SNARE complex |
| GO:0042147 retrograde transport, endosome to Golgi | IDA PMID:15215310 Participation of the syntaxin 5/Ykt6/GS28/GS15 SNARE complex... | ACCEPT | Summary: Accepted for VTI1A on independent endosome-to-Golgi evidence; the cited PMID appears to describe GS15 rather than VTI1A. Reason: VTI1A-specific evidence from UniProt and PMID:18195106 supports retrograde endosome-to-Golgi transport, but the original GS15 PMID should be reviewed as a possible source/reference error. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 PMID:15215310 syntaxin 5, GS28, Ykt6, and GS15 exist as a unique SNARE complex |
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Download this section (compressed HTML)Q: Does VTI1A directly mediate phagophore closure/autophagosome assembly, or is the observed autophagy phenotype an indirect consequence of STX13-associated endosomal SNARE trafficking?
Q: Should VTI1A annotations citing PMID:15215310 be migrated to the GS15/BET1L gene rather than VTI1A?
Q: Which VTI1A-containing SNARE complex is active in autophagy: STX13/STX12-VTI1A, STX10-STX16-VTI1A-VAMP3, VAMP7-VTI1A, or a distinct context-specific complex?
Experiment: Rescue VTI1A knockdown with wild-type and SNARE-domain/membrane-anchor mutants in MPR recycling, Golgi ribbon, and autophagic flux assays.
Hypothesis: VTI1A SNARE activity and membrane targeting are required for both endosome-to-TGN transport and autophagy-associated trafficking.
Experiment: Use endogenous tagging/proximity labeling during starvation or CHMP2B perturbation to identify VTI1A SNARE partners at autophagy-related membranes.
Hypothesis: VTI1A forms a context-specific SNARE complex during autophagic flux that can be separated from its canonical endosome-to-TGN complex.
Experiment: Compare VTI1A depletion with ESCRT-III perturbation for LC3 flux, phagophore closure, and ESCRT-III recruitment/turnover.
Hypothesis: VTI1A affects autophagy through membrane-fusion traffic rather than direct ESCRT-III complex membership.
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