VTI1A encodes a VTI1-family SNARE that mediates vesicle transport through interactions with target-membrane SNAREs and promotes membrane fusion. Its best-supported core function is SNAP receptor/SNARE-complex activity in retrograde trafficking from endosomes or late endosomes to the trans-Golgi network/Golgi, with related roles in Golgi/TGN membrane traffic and selected non-conventional cell-surface trafficking routes. VTI1A also has direct support in autophagy/autophagosome context through STX13/Vti1a-associated autophagic flux, but it is not an ESCRT-III complex component.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0006891 intra-Golgi vesicle-mediated transport | IBA GO_REF:0000033 | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: intra-Golgi vesicle-mediated transport. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0006896 Golgi to vacuole transport | IBA GO_REF:0000033 | MODIFY | Summary: The yeast-derived Golgi-to-vacuole term captures a trafficking idea but is not the best human VTI1A process term. Reason: Human evidence supports endosome/late-endosome to trans-Golgi network retrograde transport rather than a literal Golgi-to-vacuole process. Proposed replacements: retrograde transport, endosome to Golgi Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 |
| GO:0016236 macroautophagy | IBA GO_REF:0000033 | ACCEPT | Summary: Directly supported autophagy/autophagosome context for VTI1A: macroautophagy. Reason: PMID:24095276 directly reports Vti1a knockdown blocking autophagic flux in the STX13/CHMP2B context and GOA includes autophagosome localization from that study. Supporting Evidence: PMID:24095276 Knockdown of syntaxin 13 (STX13) or its binding partner Vti1a in mammalian cells caused LC3-positive puncta to accumulate PMID:24095276 blocks autophagic flux |
| GO:0042147 retrograde transport, endosome to Golgi | IBA GO_REF:0000033 | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: retrograde transport, endosome to Golgi. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0048280 vesicle fusion with Golgi apparatus | IBA GO_REF:0000033 | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: vesicle fusion with Golgi apparatus. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0000149 SNARE binding | IBA GO_REF:0000033 | MODIFY | Summary: The annotation reflects SNARE-partner interactions, but SNAP receptor activity is the more informative molecular-function term for VTI1A. Reason: VTI1A is a SNARE whose core molecular function is SNAP receptor/SNARE complex activity in membrane fusion; SNARE binding alone is less precise. Proposed replacements: SNAP receptor activity Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport |
| GO:0005484 SNAP receptor activity | IBA GO_REF:0000033 | ACCEPT | Summary: Supported as the core VTI1A molecular function. Reason: VTI1A is a SNARE/SNAP receptor that forms cognate SNARE complexes for membrane fusion and vesicle transport. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0005789 endoplasmic reticulum membrane | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: endoplasmic reticulum membrane. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0012507 ER to Golgi transport vesicle membrane | IBA GO_REF:0000033 | ACCEPT | Summary: Supported location for VTI1A in non-conventional ER/Golgi or trafficking-vesicle routes. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 PMID:19138172 inhibited Kv4/KChIP1traffic to the plasma membrane UniProt:Q96AJ9 Cytoplasmic vesicle |
| GO:0031902 late endosome membrane | IBA GO_REF:0000033 | ACCEPT | Summary: Supported compartment for VTI1A SNARE-mediated trafficking: late endosome membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Cytoplasmic vesicle |
| GO:0000139 Golgi membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: Golgi membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0005484 SNAP receptor activity | IEA GO_REF:0000002 | ACCEPT | Summary: Supported as the core VTI1A molecular function. Reason: VTI1A is a SNARE/SNAP receptor that forms cognate SNARE complexes for membrane fusion and vesicle transport. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0005737 cytoplasm | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: cytoplasm. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0005794 Golgi apparatus | IEA GO_REF:0000002 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: Golgi apparatus. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0005829 cytosol | IEA GO_REF:0000108 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: cytosol. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0006886 intracellular protein transport | IEA GO_REF:0000002 | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: intracellular protein transport. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0012505 endomembrane system | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: endomembrane system. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0016020 membrane | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: membrane. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0016192 vesicle-mediated transport | IEA GO_REF:0000002 | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: vesicle-mediated transport. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0016197 endosomal transport | IEA GO_REF:0000117 | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: endosomal transport. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0031410 cytoplasmic vesicle | IEA GO_REF:0000044 | ACCEPT | Summary: Supported cytoplasmic vesicle localization for VTI1A trafficking routes. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Cytoplasmic vesicle PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0061025 membrane fusion | IEA GO_REF:0000117 | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: membrane fusion. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0098588 bounding membrane of organelle | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: bounding membrane of organelle. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0032456 endocytic recycling | IMP PMID:23677696 VAMP4 is required to maintain the ribbon structure of the Go... | MODIFY | Summary: The phenotype in the cited Golgi-ribbon paper is better described as early-endosome-to-TGN retrograde traffic than generic endocytic recycling. Reason: PMID:23677696 links Vti1a to the VAMP4/STX6/STX16 SNARE pathway for early-endosome-to-TGN retrograde trafficking and Golgi organization. Proposed replacements: retrograde transport, endosome to Golgi Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN |
| GO:0005515 protein binding | IPI PMID:24095276 Syntaxin 13, a genetic modifier of mutant CHMP2B in frontote... | MARK AS OVER ANNOTATED | Summary: The STX12/VTI1A interaction is relevant, but GO:0005515 is too generic for VTI1A molecular function. Reason: The useful curation should describe VTI1A as a SNAP receptor/SNARE component in membrane traffic and autophagy-associated trafficking, not generic protein binding. Supporting Evidence: file:human/VTI1A/VTI1A-notes.md Generic protein binding is too vague for VTI1A; the useful function is SNARE-mediated membrane traffic. PMID:24095276 Knockdown of syntaxin 13 (STX13) or its binding partner Vti1a |
| GO:0005776 autophagosome | IDA PMID:24095276 Syntaxin 13, a genetic modifier of mutant CHMP2B in frontote... | ACCEPT | Summary: Directly supported autophagy/autophagosome context for VTI1A: autophagosome. Reason: PMID:24095276 directly reports Vti1a knockdown blocking autophagic flux in the STX13/CHMP2B context and GOA includes autophagosome localization from that study. Supporting Evidence: PMID:24095276 Knockdown of syntaxin 13 (STX13) or its binding partner Vti1a in mammalian cells caused LC3-positive puncta to accumulate PMID:24095276 blocks autophagic flux |
| GO:0006914 autophagy | IMP PMID:24095276 Syntaxin 13, a genetic modifier of mutant CHMP2B in frontote... | ACCEPT | Summary: Directly supported autophagy/autophagosome context for VTI1A: autophagy. Reason: PMID:24095276 directly reports Vti1a knockdown blocking autophagic flux in the STX13/CHMP2B context and GOA includes autophagosome localization from that study. Supporting Evidence: PMID:24095276 Knockdown of syntaxin 13 (STX13) or its binding partner Vti1a in mammalian cells caused LC3-positive puncta to accumulate PMID:24095276 blocks autophagic flux |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-6811431 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: trans-Golgi network membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-6814671 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: trans-Golgi network membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-6814674 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: trans-Golgi network membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-6814676 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: trans-Golgi network membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-6814678 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: trans-Golgi network membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-6814682 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: trans-Golgi network membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-6814683 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: trans-Golgi network membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0032588 trans-Golgi network membrane | TAS Reactome:R-HSA-6814684 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: trans-Golgi network membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0000139 Golgi membrane | TAS Reactome:R-HSA-6811431 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: Golgi membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0000139 Golgi membrane | TAS Reactome:R-HSA-6811433 | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: Golgi membrane. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0050882 voluntary musculoskeletal movement | IMP PMID:22958904 VAMP1 mutation causes dominant hereditary spastic ataxia in ... | UNDECIDED | Summary: Mutant-phenotype annotation citing the study of a VAMP1 mutation in hereditary spastic ataxia (PMID:22958904; abstract only), whose abstract does not mention VTI1A. Reason: The cited paper identifies a VAMP1 mutation as the cause of a dominant hereditary spastic ataxia, and its abstract does not mention VTI1A; only the abstract is available. The annotation may have been attached to the wrong SNARE, but whether the full text reports an experiment involving VTI1A cannot be checked, and an experimental annotation is not treated as a mis-attribution on the strength of an abstract. The row is left undecided, and the possible error is noted for the source. Supporting Evidence: PMID:22958904 identifies vesicle-associated membrane protein 1 (VAMP1) |
| GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport | ISS GO_REF:0000024 | ACCEPT | Summary: Supported as a non-conventional ER/Golgi or early secretory trafficking context for VTI1A. Reason: PMID:19138172 supports a VAMP7/Vti1a route for KChIP1/Kv4 traffic distinct from conventional VSVG traffic. Supporting Evidence: PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 PMID:19138172 inhibited Kv4/KChIP1traffic to the plasma membrane UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers |
| GO:0048280 vesicle fusion with Golgi apparatus | ISS GO_REF:0000024 | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: vesicle fusion with Golgi apparatus. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0005768 endosome | ISS GO_REF:0000024 | ACCEPT | Summary: Supported compartment for VTI1A SNARE-mediated trafficking: endosome. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Cytoplasmic vesicle |
| GO:0008021 synaptic vesicle | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: synaptic vesicle. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0030136 clathrin-coated vesicle | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: clathrin-coated vesicle. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0031201 SNARE complex | ISS GO_REF:0000024 | ACCEPT | Summary: Supported as a core SNARE-complex context for VTI1A. Reason: VTI1A is part of STX10/STX16/VAMP3 and VAMP4/STX6/STX16 SNARE-complex contexts that mediate endosome-to-Golgi/TGN traffic. Supporting Evidence: PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Identified in a complex containing STX6, STX12, VAMP4 and VTI1A |
| GO:0043025 neuronal cell body | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: neuronal cell body. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0044306 neuron projection terminus | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: neuron projection terminus. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0048471 perinuclear region of cytoplasm | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: True or plausible but broad/specialized VTI1A location: perinuclear region of cytoplasm. Reason: More specific Golgi/TGN, late endosome/endosome, cytoplasmic vesicle, autophagosome, and SNARE-complex annotations capture the core function better. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:19138172 KChIP1-expressing vesicles co-localized with the SNARE proteins Vti1a and VAMP7 |
| GO:0005794 Golgi apparatus | IDA PMID:19224922 Differential effects of depletion of ARL1 and ARFRP1 on memb... | ACCEPT | Summary: Supported Golgi/TGN location for VTI1A: Golgi apparatus. Reason: This location matches VTI1A SNARE function in Golgi/TGN, endosomal, or trafficking-vesicle membranes. Supporting Evidence: UniProt:Q96AJ9 Golgi apparatus membrane PMID:23677696 syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure PMID:23677696 normal retrograde trafficking from the early endosome to the TGN UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0042147 retrograde transport, endosome to Golgi | IMP PMID:19224922 Differential effects of depletion of ARL1 and ARFRP1 on memb... | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: retrograde transport, endosome to Golgi. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0042147 retrograde transport, endosome to Golgi | IDA PMID:18195106 A syntaxin 10-SNARE complex distinguishes two distinct trans... | ACCEPT | Summary: Supported core VTI1A SNARE-mediated trafficking process: retrograde transport, endosome to Golgi. Reason: VTI1A participates in SNARE complexes mediating vesicle fusion and retrograde endosome-to-Golgi/TGN traffic. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Golgi apparatus membrane |
| GO:0005484 SNAP receptor activity | IDA PMID:15215310 Participation of the syntaxin 5/Ykt6/GS28/GS15 SNARE complex... | ACCEPT | Summary: Accepted for VTI1A on independent SNARE evidence; the cited PMID appears to describe GS15 rather than VTI1A. Reason: VTI1A clearly has SNAP receptor activity from UniProt and other VTI1A-specific evidence, but the original GS15 PMID should be reviewed as a possible source/reference error. Supporting Evidence: UniProt:Q96AJ9 mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane UniProt:Q96AJ9 to promote fusion of the lipid bilayers PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:15215310 syntaxin 5, GS28, Ykt6, and GS15 exist as a unique SNARE complex |
| GO:0031201 SNARE complex | TAS PMID:15215310 Participation of the syntaxin 5/Ykt6/GS28/GS15 SNARE complex... | ACCEPT | Summary: Accepted for VTI1A on independent SNARE-complex evidence; the cited PMID appears to describe GS15 rather than VTI1A. Reason: VTI1A is supported as a SNARE-complex component by VTI1A-specific evidence, but the original GS15 PMID should be reviewed as a possible source/reference error. Supporting Evidence: PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 UniProt:Q96AJ9 Identified in a complex containing STX6, STX12, VAMP4 and VTI1A PMID:15215310 syntaxin 5, GS28, Ykt6, and GS15 exist as a unique SNARE complex |
| GO:0042147 retrograde transport, endosome to Golgi | IDA PMID:15215310 Participation of the syntaxin 5/Ykt6/GS28/GS15 SNARE complex... | ACCEPT | Summary: Accepted for VTI1A on independent endosome-to-Golgi evidence; the cited PMID appears to describe GS15 rather than VTI1A. Reason: VTI1A-specific evidence from UniProt and PMID:18195106 supports retrograde endosome-to-Golgi transport, but the original GS15 PMID should be reviewed as a possible source/reference error. Supporting Evidence: UniProt:Q96AJ9 Involved in vesicular transport from the late endosomes to the trans-Golgi network PMID:18195106 complex comprised of syntaxin 10 (STX10), STX16, Vti1a, and VAMP3 is required for this MPR transport PMID:18195106 implicate a SNARE complex comprised of STX10, STX16, Vti1a, and VAMP3 PMID:15215310 syntaxin 5, GS28, Ykt6, and GS15 exist as a unique SNARE complex |
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Download this section (compressed HTML)Q: Does VTI1A directly mediate phagophore closure/autophagosome assembly, or is the observed autophagy phenotype an indirect consequence of STX13-associated endosomal SNARE trafficking?
Q: Should VTI1A annotations citing PMID:15215310 be migrated to the GS15/BET1L gene rather than VTI1A?
Q: Which VTI1A-containing SNARE complex is active in autophagy: STX13/STX12-VTI1A, STX10-STX16-VTI1A-VAMP3, VAMP7-VTI1A, or a distinct context-specific complex?
Experiment: Rescue VTI1A knockdown with wild-type and SNARE-domain/membrane-anchor mutants in MPR recycling, Golgi ribbon, and autophagic flux assays.
Hypothesis: VTI1A SNARE activity and membrane targeting are required for both endosome-to-TGN transport and autophagy-associated trafficking.
Experiment: Use endogenous tagging/proximity labeling during starvation or CHMP2B perturbation to identify VTI1A SNARE partners at autophagy-related membranes.
Hypothesis: VTI1A forms a context-specific SNARE complex during autophagic flux that can be separated from its canonical endosome-to-TGN complex.
Experiment: Compare VTI1A depletion with ESCRT-III perturbation for LC3 flux, phagophore closure, and ESCRT-III recruitment/turnover.
Hypothesis: VTI1A affects autophagy through membrane-fusion traffic rather than direct ESCRT-III complex membership.
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