ZDHHC23

UniProt ID: Q8IYP9
Organism: Homo sapiens
Review Status: IN PROGRESS
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Gene Description

ZDHHC23 is a membrane-associated DHHC protein S-palmitoyltransferase. It contributes to reversible lipidation of protein substrates, including the BK potassium-channel subunit KCNMA1, and helps regulate delivery of that channel to the cell surface.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0000139 Golgi membrane
IEA
GO_REF:0000044 		ACCEPT
Summary: The characterized DHHC palmitoyltransferase localizes to Golgi membranes, consistent with substrate lipidation in the secretory pathway.
Reason: The characterized DHHC palmitoyltransferase localizes to Golgi membranes, consistent with substrate lipidation in the secretory pathway.
Supporting Evidence:
file:human/ZDHHC23/ZDHHC23-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}. Golgi apparatus, trans-Golgi network membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}.
PMID:22399288
Here, we demonstrate that palmitoylation of the intracellular S0-S1 loop of BK channels is controlled by two of the 23 mammalian palmitoyl-transferases, zDHHC22 and zDHHC23. Palmitoylation by these acyl transferases is essential for efficient cell surface expression of BK channels.
GO:0005515 protein binding
IPI
PMID:32296183
A reference map of the human binary protein interactome. 		KEEP AS NON CORE
Summary: The high-throughput interaction observation is retained as a physical interaction but the generic protein-binding term does not specify the catalytic function.
Reason: The high-throughput interaction observation is retained as a physical interaction but the generic protein-binding term does not specify the catalytic function.
Supporting Evidence:
file:human/ZDHHC23/ZDHHC23-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}. Golgi apparatus, trans-Golgi network membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}.
GO:0005783 endoplasmic reticulum
IBA
GO_REF:0000033 		ACCEPT
Summary: The curated phylogenetic ER localization is compatible with the secretory-membrane DHHC protein; it is not contradicted by its additional Golgi localization.
Reason: The curated phylogenetic ER localization is compatible with the secretory-membrane DHHC protein; it is not contradicted by its additional Golgi localization.
Supporting Evidence:
file:human/ZDHHC23/ZDHHC23-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}. Golgi apparatus, trans-Golgi network membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}.
GO:0005794 Golgi apparatus
IBA
GO_REF:0000033 		ACCEPT
Summary: Golgi localization fits the experimentally investigated membrane palmitoylation machinery.
Reason: Golgi localization fits the experimentally investigated membrane palmitoylation machinery.
Supporting Evidence:
file:human/ZDHHC23/ZDHHC23-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}. Golgi apparatus, trans-Golgi network membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}.
PMID:22399288
Here, we demonstrate that palmitoylation of the intracellular S0-S1 loop of BK channels is controlled by two of the 23 mammalian palmitoyl-transferases, zDHHC22 and zDHHC23. Palmitoylation by these acyl transferases is essential for efficient cell surface expression of BK channels.
GO:0005794 Golgi apparatus
IEA
GO_REF:0000044 		ACCEPT
Summary: Golgi localization fits the experimentally investigated membrane palmitoylation machinery.
Reason: Golgi localization fits the experimentally investigated membrane palmitoylation machinery.
Supporting Evidence:
file:human/ZDHHC23/ZDHHC23-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}. Golgi apparatus, trans-Golgi network membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}.
PMID:22399288
Here, we demonstrate that palmitoylation of the intracellular S0-S1 loop of BK channels is controlled by two of the 23 mammalian palmitoyl-transferases, zDHHC22 and zDHHC23. Palmitoylation by these acyl transferases is essential for efficient cell surface expression of BK channels.
GO:0006612 protein targeting to membrane
IBA
GO_REF:0000033 		ACCEPT
Summary: Palmitoylation of the BK channel S0–S1 loop facilitates its cell-surface targeting.
Reason: Palmitoylation of the BK channel S0–S1 loop facilitates its cell-surface targeting.
Supporting Evidence:
file:human/ZDHHC23/ZDHHC23-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}. Golgi apparatus, trans-Golgi network membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}.
PMID:22399288
Here, we demonstrate that palmitoylation of the intracellular S0-S1 loop of BK channels is controlled by two of the 23 mammalian palmitoyl-transferases, zDHHC22 and zDHHC23. Palmitoylation by these acyl transferases is essential for efficient cell surface expression of BK channels.
GO:0016409 palmitoyltransferase activity
IEA
GO_REF:0000002 		MODIFY
Summary: The measured reaction is S-palmitoylation of protein cysteines.
Reason: The measured reaction is S-palmitoylation of protein cysteines.
Supporting Evidence:
file:human/ZDHHC23/ZDHHC23-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}. Golgi apparatus, trans-Golgi network membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}.
PMID:22399288
Here, we demonstrate that palmitoylation of the intracellular S0-S1 loop of BK channels is controlled by two of the 23 mammalian palmitoyl-transferases, zDHHC22 and zDHHC23. Palmitoylation by these acyl transferases is essential for efficient cell surface expression of BK channels.
GO:0018345 protein palmitoylation
IMP
PMID:22399288
Distinct acyl protein transferases and thioesterases control... 		ACCEPT
Summary: Knockdown/functional experiments identify zDHHC23 as a BK-channel palmitoylation enzyme.
Reason: Knockdown/functional experiments identify zDHHC23 as a BK-channel palmitoylation enzyme.
Supporting Evidence:
file:human/ZDHHC23/ZDHHC23-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}. Golgi apparatus, trans-Golgi network membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}.
PMID:22399288
Here, we demonstrate that palmitoylation of the intracellular S0-S1 loop of BK channels is controlled by two of the 23 mammalian palmitoyl-transferases, zDHHC22 and zDHHC23. Palmitoylation by these acyl transferases is essential for efficient cell surface expression of BK channels.
GO:0019706 protein-cysteine S-palmitoyltransferase activity
EXP
PMID:22399288
Distinct acyl protein transferases and thioesterases control... 		ACCEPT
Summary: The catalytic DHHC protein mediates protein-cysteine S-palmitoylation, as supported by the BK-channel study.
Reason: The catalytic DHHC protein mediates protein-cysteine S-palmitoylation, as supported by the BK-channel study.
Supporting Evidence:
file:human/ZDHHC23/ZDHHC23-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}. Golgi apparatus, trans-Golgi network membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}.
PMID:22399288
Here, we demonstrate that palmitoylation of the intracellular S0-S1 loop of BK channels is controlled by two of the 23 mammalian palmitoyl-transferases, zDHHC22 and zDHHC23. Palmitoylation by these acyl transferases is essential for efficient cell surface expression of BK channels.
GO:0019706 protein-cysteine S-palmitoyltransferase activity
IBA
GO_REF:0000033 		ACCEPT
Summary: The catalytic DHHC protein mediates protein-cysteine S-palmitoylation, as supported by the BK-channel study.
Reason: The catalytic DHHC protein mediates protein-cysteine S-palmitoylation, as supported by the BK-channel study.
Supporting Evidence:
file:human/ZDHHC23/ZDHHC23-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}. Golgi apparatus, trans-Golgi network membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}.
PMID:22399288
Here, we demonstrate that palmitoylation of the intracellular S0-S1 loop of BK channels is controlled by two of the 23 mammalian palmitoyl-transferases, zDHHC22 and zDHHC23. Palmitoylation by these acyl transferases is essential for efficient cell surface expression of BK channels.
GO:0019706 protein-cysteine S-palmitoyltransferase activity
IEA
GO_REF:0000120 		ACCEPT
Summary: The catalytic DHHC protein mediates protein-cysteine S-palmitoylation, as supported by the BK-channel study.
Reason: The catalytic DHHC protein mediates protein-cysteine S-palmitoylation, as supported by the BK-channel study.
Supporting Evidence:
file:human/ZDHHC23/ZDHHC23-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}. Golgi apparatus, trans-Golgi network membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}.
PMID:22399288
Here, we demonstrate that palmitoylation of the intracellular S0-S1 loop of BK channels is controlled by two of the 23 mammalian palmitoyl-transferases, zDHHC22 and zDHHC23. Palmitoylation by these acyl transferases is essential for efficient cell surface expression of BK channels.
GO:0072659 protein localization to plasma membrane
IBA
GO_REF:0000033 		ACCEPT
Summary: The BK-channel experiments link zDHHC23-dependent palmitoylation to efficient channel surface expression.
Reason: The BK-channel experiments link zDHHC23-dependent palmitoylation to efficient channel surface expression.
Supporting Evidence:
file:human/ZDHHC23/ZDHHC23-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}. Golgi apparatus, trans-Golgi network membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}.
PMID:22399288
Here, we demonstrate that palmitoylation of the intracellular S0-S1 loop of BK channels is controlled by two of the 23 mammalian palmitoyl-transferases, zDHHC22 and zDHHC23. Palmitoylation by these acyl transferases is essential for efficient cell surface expression of BK channels.
GO:0072659 protein localization to plasma membrane
IMP
PMID:22399288
Distinct acyl protein transferases and thioesterases control... 		ACCEPT
Summary: The BK-channel experiments link zDHHC23-dependent palmitoylation to efficient channel surface expression.
Reason: The BK-channel experiments link zDHHC23-dependent palmitoylation to efficient channel surface expression.
Supporting Evidence:
file:human/ZDHHC23/ZDHHC23-uniprot.txt
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}. Golgi apparatus, trans-Golgi network membrane CC {ECO:0000305|PubMed:22399288}; Multi-pass membrane protein CC {ECO:0000255}.
PMID:22399288
Here, we demonstrate that palmitoylation of the intracellular S0-S1 loop of BK channels is controlled by two of the 23 mammalian palmitoyl-transferases, zDHHC22 and zDHHC23. Palmitoylation by these acyl transferases is essential for efficient cell surface expression of BK channels.

Core Functions

S-palmitoylation of protein substrates, including KCNMA1, supports their membrane trafficking.

Molecular Function:
protein-cysteine S-palmitoyltransferase activity
Supporting Evidence:
  • PMID:22399288
    Here, we demonstrate that palmitoylation of the intracellular S0-S1 loop of BK channels is controlled by two of the 23 mammalian palmitoyl-transferases, zDHHC22 and zDHHC23. Palmitoylation by these acyl transferases is essential for efficient cell surface expression of BK channels.

References

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Deep Research

Falcon

(ZDHHC23-deep-research-falcon.md)

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Manual

(ZDHHC23-deep-research-manual.md)

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📚 Additional Documentation

Notes

(ZDHHC23-notes.md)

ZDHHC23: evidence notes

Paired horse benchmark evidence review

The human reference supplies mechanistic evidence for the corresponding selected horse protein; the human conclusion alone is not validation of the horse sequence. The exact horse comparison is in genes/HORSE/ZDHHC23/ZDHHC23-bioinformatics/RESULTS.md. Research reports are source leads; annotation decisions cite the underlying publication or experimentally supported UniProt passages. Unresolved source-specific results retain UNDECIDED.

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