ZNF788P

Summary: This row comes from endogenous CD-tagging (YFP inserted as an internal exon at the chromosomal locus in H1299 cells), not from overexpression. Clone "FLJ46419" (the ZNF788P alias) was one of 20 nuclear proteins tracked. The evidence is real, but it was attached in 2015 to the former 615-aa zinc-finger sequence of this entry, not to the current 82-aa KRAB fragment.

Reason: I read the full text (author-hosted PDF; the publications cache holds only the abstract). Each tagged protein was identified by 3' RACE from YFP to the poly(A) tail, and Fig. 3q lists "uncharacterized protein FLJ46419" as nuclear. A promoterless, ATG-less YFP exon fluoresces only when spliced in frame into a translated mRNA. The observation is therefore direct evidence that some protein encoded by the FLJ46419 transcript is translated and nuclear. Because 3' RACE reads downstream of the insertion, the tagged protein most likely includes the zinc-finger region (the pre-2018 UniProt sequence) and not the current 82-aa KRAB-A fragment, which has no nuclear-targeting zinc fingers. This conflicts with HGNC and Ensembl, which classify the locus as a pseudogene, and with UniProt's PE5 and 2018 remodelling. The 3' RACE read could also have matched a paralogous chr19 zinc-finger gene, and that cannot be checked from the paper. The annotation is neither removed nor accepted. UniProt should reconcile the HDA with the current sequence.

Summary: InterPro2GO mapping from a truncated KRAB-A box on a pseudogene product. The product has no zinc fingers or any other DNA-binding domain, no KRAB-B box, and a substitution in the KRAB-A MLE motif.

Reason: A KRAB domain represses transcription only when it is tethered to DNA, which in KRAB-zinc-finger proteins is done by the C2H2 array. The annotated 82-aa product lacks any DNA-binding domain. Its KRAB-A box ends at the equivalent of ZNF10 residue 68, so it lacks the end of the A box and the whole B box, and the central leucine of the conserved MLE motif is replaced by glutamine. The locus is classified as a pseudogene by HGNC and Ensembl, and protein existence is PE5. The InterPro signature correctly recognises a KRAB-A remnant, but that does not show participation in transcription regulation.