id: P60766
gene_symbol: Cdc42
product_type: PROTEIN
status: COMPLETE
taxon:
  id: NCBITaxon:10090
  label: Mus musculus
description: 'Cdc42 is a conserved Rho-family small GTPase that functions as a GDP/GTP-regulated
  molecular switch. Active GTP-bound Cdc42 binds effectors such as WASP/N-WASP, PAK, and Par6/aPKC
  polarity complexes to organize cortical actin, filopodia, cell polarity, junctions, migration,
  endocytosis, cytokinesis, and spindle-related processes. Its outputs depend strongly on
  membrane targeting and isoform-specific C-terminal lipidation: the broadly expressed prenylated
  isoform acts at leading-edge/cortical membranes, while a brain-enriched palmitoylated isoform
  contributes to intracellular membrane and neuronal contexts.'
alternative_products:
- name: 2 (Placental)
  id: P60766-2, P21181-4
- name: 1 (Brain)
  id: P60766-1, P21181-1
  sequence_note: VSP_040585, VSP_040586
references:
- id: GO_REF:0000002
  title: Gene Ontology annotation through association of InterPro records with GO terms
  findings: []
- id: GO_REF:0000003
  title: Gene Ontology annotation based on Enzyme Commission mapping
  findings: []
- id: GO_REF:0000024
  title: Manual transfer of experimentally-verified manual GO annotation data to orthologs
    by curator judgment of sequence similarity
  findings: []
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
- id: GO_REF:0000043
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
  findings: []
- id: GO_REF:0000044
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary
    mapping, accompanied by conservative changes to GO terms applied by UniProt
  findings: []
- id: GO_REF:0000096
  title: Automated transfer of experimentally-verified manual GO annotation data to mouse-rat
    orthologs
  findings: []
- id: GO_REF:0000107
  title: Automatic transfer of experimentally verified manual GO annotation data to orthologs
    using Ensembl Compara
  findings: []
- id: GO_REF:0000117
  title: Electronic Gene Ontology annotations created by ARBA machine learning models
  findings: []
- id: GO_REF:0000119
  title: Automated transfer of experimentally-verified manual GO annotation data to mouse-human
    orthologs
  findings: []
- id: GO_REF:0000120
  title: Combined Automated Annotation using Multiple IEA Methods
  findings: []
- id: PMID:10490598
  title: The Borgs, a new family of Cdc42 and TC10 GTPase-interacting proteins.
  findings: []
- id: PMID:10699171
  title: Functional analysis of ARHGAP6, a novel GTPase-activating protein for RhoA.
  findings: []
- id: PMID:10954424
  title: The mammalian homologue of the Caenorhabditis elegans polarity protein PAR-6 is a
    binding partner for the Rho GTPases Cdc42 and Rac1.
  findings: []
- id: PMID:11035016
  title: A new family of Cdc42 effector proteins, CEPs, function in fibroblast and epithelial
    cell shape changes.
  findings: []
- id: PMID:12123608
  title: Plexin-B1 directly interacts with PDZ-RhoGEF/LARG to regulate RhoA and growth cone
    morphology.
  findings: []
- id: PMID:12637522
  title: Multifunctional roles for the PH domain of Dbs in regulating Rho GTPase activation.
  findings: []
- id: PMID:12773384
  title: TCGAP, a multidomain Rho GTPase-activating protein involved in insulin-stimulated
    glucose transport.
  findings: []
- id: PMID:15249579
  title: Vav GEFs are required for beta2 integrin-dependent functions of neutrophils.
  findings: []
- id: PMID:15537656
  title: A direct interaction between Cdc42 and vesicle-associated membrane protein 2 regulates
    SNARE-dependent insulin exocytosis.
  findings: []
- id: PMID:15710388
  title: 'Zizimin2: a novel, DOCK180-related Cdc42 guanine nucleotide exchange factor expressed
    predominantly in lymphocytes.'
  findings: []
- id: PMID:15728722
  title: Local phosphatidylinositol 3,4,5-trisphosphate accumulation recruits Vav2 and Vav3
    to activate Rac1/Cdc42 and initiate neurite outgrowth in nerve growth factor-stimulated
    PC12 cells.
  findings: []
- id: PMID:15775979
  title: E-cadherin is essential for in vivo epidermal barrier function by regulating tight
    junctions.
  findings: []
- id: PMID:15882626
  title: Nuclear movement regulated by Cdc42, MRCK, myosin, and actin flow establishes MTOC
    polarization in migrating cells.
  findings: []
- id: PMID:15996550
  title: The Rho-specific GEF Lfc interacts with neurabin and spinophilin to regulate dendritic
    spine morphology.
  findings: []
- id: PMID:16052498
  title: Expression, synaptic localization, and developmental regulation of Ack1/Pyk1, a cytoplasmic
    tyrosine kinase highly expressed in the developing and adult brain.
  findings: []
- id: PMID:16621792
  title: 'Membrane localization of adenomatous polyposis coli protein at cellular protrusions:
    targeting sequences and regulation by beta-catenin.'
  findings: []
- id: PMID:16892058
  title: The Rho-GTPase cdc42 regulates neural progenitor fate at the apical surface.
  findings: []
- id: PMID:17000758
  title: Ccpg1, a novel scaffold protein that regulates the activity of the Rho guanine nucleotide
    exchange factor Dbs.
  findings: []
- id: PMID:17634366
  title: Proteolipid protein is required for transport of sirtuin 2 into CNS myelin.
  findings: []
- id: PMID:18325335
  title: Affixin activates Rac1 via betaPIX in C2C12 myoblast.
  findings: []
- id: PMID:18541910
  title: Bves directly interacts with GEFT, and controls cell shape and movement through regulation
    of Rac1/Cdc42 activity.
  findings: []
- id: PMID:18604197
  title: IQGAP3 regulates cell proliferation through the Ras/ERK signalling cascade.
  findings: []
- id: PMID:18838382
  title: FGD2, a CDC42-specific exchange factor expressed by antigen-presenting cells, localizes
    to early endosomes and active membrane ruffles.
  findings: []
- id: PMID:18854161
  title: ACF7 regulates cytoskeletal-focal adhesion dynamics and migration and has ATPase
    activity.
  findings: []
- id: PMID:19144319
  title: The phagosomal proteome in interferon-gamma-activated macrophages.
  findings: []
- id: PMID:19244314
  title: Cdo promotes neuronal differentiation via activation of the p38 mitogen-activated
    protein kinase pathway.
  findings: []
- id: PMID:19796622
  title: Uncovering early response of gene regulatory networks in ESCs by systematic induction
    of transcription factors.
  findings: []
- id: PMID:19893577
  title: The adenomatous polyposis coli-associated exchange factors Asef and Asef2 are required
    for adenoma formation in Apc(Min/+)mice.
  findings: []
- id: PMID:21048939
  title: ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange factor for leucine-rich repeat
    kinase 2.
  findings: []
- id: PMID:21690310
  title: Tinman/Nkx2-5 acts via miR-1 and upstream of Cdc42 to regulate heart function across
    species.
  findings: []
- id: PMID:21828338
  title: "Cool-1/\u03B2PIX functions as a guanine nucleotide exchange factor in the cycling\
    \ of Cdc42 to regulate insulin secretion."
  findings: []
- id: PMID:22461490
  title: DOCK8 is a Cdc42 activator critical for interstitial dendritic cell migration during
    immune responses.
  findings: []
- id: PMID:22494997
  title: Age-related guanine nucleotide exchange factor, mouse Zizimin2, induces filopodia
    in bone marrow-derived dendritic cells.
  findings: []
- id: PMID:23219958
  title: CDC42 is required for structural patterning of the lung during development.
  findings: []
- id: PMID:23620790
  title: CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-cell adhesion
    and migration.
  findings: []
- id: PMID:23706742
  title: RAS and RHO families of GTPases directly regulate distinct phosphoinositide 3-kinase
    isoforms.
  findings: []
- id: PMID:23793062
  title: The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation
    via CD28 and the development of regulatory T cells.
  findings: []
- id: PMID:24076653
  title: CDC42 switches IRSp53 from inhibition of actin growth to elongation by clustering
    of VASP.
  findings: []
- id: PMID:24792215
  title: CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and actin-dependent
    events.
  findings: []
- id: PMID:25197047
  title: Essential role of sympathetic endothelin A receptors for adverse cardiac remodeling.
  findings: []
- id: PMID:25217619
  title: Phosphoinositide 3-kinase p85beta regulates invadopodium formation.
  findings: []
- id: PMID:25851601
  title: The RhoGEF DOCK10 is essential for dendritic spine morphogenesis.
  findings: []
- id: PMID:27917469
  title: Association of dysfunctional synapse defective 1 (SYDE1) with restricted fetal growth
    - SYDE1 regulates placental cell migration and invasion.
  findings: []
- id: PMID:31219639
  title: Cdc42 activation by endothelin regulates neural crest cell migration in the cardiac
    outflow tract.
  findings: []
- id: PMID:33531495
  title: Photoactivatable CaMKII induces synaptic plasticity in single synapses.
  findings: []
- id: PMID:37140151
  title: Biallelic variants in IQCN cause sperm flagellar assembly defects and male infertility.
  findings: []
- id: PMID:8464478
  title: Oncogene ect2 is related to regulators of small GTP-binding proteins.
  findings: []
- id: Reactome:R-MMU-3928643
  title: Activation of CDC42 by intersectin-1
  findings: []
- id: Reactome:R-MMU-442572
  title: Activation of WASP and N-WASP by CDC42
  findings: []
- id: Reactome:R-MMU-448947
  title: Interaction of Bnip-2 with Cdc42
  findings: []
- id: Reactome:R-MMU-5665684
  title: Fmnl1 binds Cdc42:GTP
  findings: []
- id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
  title: Falcon deep research summary for mouse Cdc42
  findings: []
existing_annotations:
- term:
    id: GO:0003924
    label: GTPase activity
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0005525
    label: GTP binding
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: GTP binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or
      localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0007165
    label: signal transduction
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: signal transduction is a broad ancestor for the Cdc42 signaling role.
    action: MODIFY
    reason: Cdc42 is best represented as a small GTPase/Rho-family signaling switch.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 belongs to the **Rho family** of small GTPases and acts as a
        **molecular switch** cycling between **GTP-bound (active)** and **GDP-bound (inactive)**
        states
    proposed_replacement_terms:
    - &id001
      id: GO:0007264
      label: small GTPase-mediated signal transduction
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0007015
    label: actin filament organization
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: actin filament organization is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0030010
    label: establishment of cell polarity
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: establishment of cell polarity is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42b supports **N-WASP-mediated endocytosis** from intracellular
        compartments.
- term:
    id: GO:0006897
    label: endocytosis
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: endocytosis is a supported Cdc42 cellular output, but it is not the core small
      GTPase catalytic function.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 GTP-bound effector signaling can influence trafficking and membrane dynamics,
      but this process should be treated as context-specific.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0019901
    label: protein kinase binding
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: protein kinase binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0051489
    label: regulation of filopodium assembly
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review:
    summary: regulation of filopodium assembly is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0000166
    label: nucleotide binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000043
  review:
    summary: Nucleotide binding is too broad for Cdc42.
    action: MODIFY
    reason: Cdc42 specifically binds guanine nucleotides and functions through GTP/GDP cycling.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
    proposed_replacement_terms:
    - id: GO:0005525
      label: GTP binding
- term:
    id: GO:0003924
    label: GTPase activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0003925
    label: G protein activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000003
  review:
    summary: G protein activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0005525
    label: GTP binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: GTP binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or
      localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: cytoplasm is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization
      biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0005813
    label: centrosome
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: centrosome is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization
      biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0005819
    label: spindle
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  review:
    summary: This automated generic cellular-component transfer is too broad relative
      to the narrower Cdc42 localization annotations retained elsewhere.
    action: REMOVE
    reason: The local evidence does not justify keeping this broad automated component
      row as a separate accepted annotation; it was previously supported only by
      unrelated actin-effector biology.
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0007264
    label: small GTPase-mediated signal transduction
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  review:
    summary: small GTPase-mediated signal transduction is consistent with core Cdc42 GTPase,
      cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0007399
    label: nervous system development
  evidence_type: IEA
  original_reference_id: GO_REF:0000043
  review:
    summary: nervous system development is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0009653
    label: anatomical structure morphogenesis
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: anatomical structure morphogenesis is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0016787
    label: hydrolase activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000043
  review:
    summary: Hydrolase activity is too broad for Cdc42.
    action: MODIFY
    reason: The relevant hydrolytic activity is GTPase activity in a Rho-family molecular
      switch.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
    proposed_replacement_terms:
    - &id002
      id: GO:0003924
      label: GTPase activity
- term:
    id: GO:0030154
    label: cell differentiation
  evidence_type: IEA
  original_reference_id: GO_REF:0000043
  review:
    summary: cell differentiation is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0030425
    label: dendrite
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  review:
    summary: dendrite is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0030496
    label: midbody
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: midbody is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization
      biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0031258
    label: lamellipodium membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  review:
    summary: lamellipodium membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0051130
    label: positive regulation of cellular component organization
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: positive regulation of cellular component organization is a supported context-specific
      Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0071944
    label: cell periphery
  evidence_type: IEA
  original_reference_id: GO_REF:0000117
  review:
    summary: cell periphery is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:12123608
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:15537656
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:18325335
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:18541910
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:18854161
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:19893577
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:23706742
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0006911
    label: phagocytosis, engulfment
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: phagocytosis, engulfment is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0007015
    label: actin filament organization
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: actin filament organization is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0007229
    label: integrin-mediated signaling pathway
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: integrin-mediated signaling pathway is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0010591
    label: regulation of lamellipodium assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: regulation of lamellipodium assembly is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0010592
    label: positive regulation of lamellipodium assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: positive regulation of lamellipodium assembly is consistent with core Cdc42 GTPase,
      cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0015630
    label: microtubule cytoskeleton
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: microtubule cytoskeleton is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0016020
    label: membrane
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization
      biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0017119
    label: Golgi transport complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: Golgi transport complex is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0019901
    label: protein kinase binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: protein kinase binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0030036
    label: actin cytoskeleton organization
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: actin cytoskeleton organization is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0030175
    label: filopodium
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: filopodium is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization
      biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0030307
    label: positive regulation of cell growth
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: positive regulation of cell growth is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0030335
    label: positive regulation of cell migration
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: positive regulation of cell migration is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0031274
    label: positive regulation of pseudopodium assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: positive regulation of pseudopodium assembly is consistent with core Cdc42 GTPase,
      cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0031333
    label: negative regulation of protein-containing complex assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: negative regulation of protein-containing complex assembly is a supported context-specific
      Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0031996
    label: thioesterase binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: thioesterase binding is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0032427
    label: GBD domain binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: GBD domain binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0032467
    label: positive regulation of cytokinesis
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: positive regulation of cytokinesis is supported for Cdc42, but this is a cell-cycle
      output rather than the small GTPase catalytic role itself.
    action: ACCEPT
    reason: UniProt carries this transferred process annotation, but it is treated as a
      downstream cell-cycle output rather than core GTPase catalysis.
    supported_by:
    - reference_id: UniProt:P60766
      supporting_text: 'GO; GO:0032467; P:positive regulation of cytokinesis; ISS:UniProtKB.'
- term:
    id: GO:0032956
    label: regulation of actin cytoskeleton organization
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: regulation of actin cytoskeleton organization is a supported context-specific
      Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0032991
    label: protein-containing complex
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: protein-containing complex is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0034191
    label: apolipoprotein A-I receptor binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: apolipoprotein A-I receptor binding is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0034329
    label: cell junction assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: cell junction assembly is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0036464
    label: cytoplasmic ribonucleoprotein granule
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: cytoplasmic ribonucleoprotein granule is retained as a non-core Cdc42-associated
      annotation.
    action: KEEP_AS_NON_CORE
    reason: The annotation is plausible as a downstream or compartment-specific consequence
      of Cdc42 signaling rather than the core GTPase switch activity.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0038189
    label: neuropilin signaling pathway
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: neuropilin signaling pathway is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0042802
    label: identical protein binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: identical protein binding is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0043005
    label: neuron projection
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: neuron projection is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0043025
    label: neuronal cell body
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: neuronal cell body is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0044788
    label: host-mediated perturbation of viral process
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: host-mediated perturbation of viral process is a supported context-specific Cdc42
      output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0045198
    label: establishment of epithelial cell apical/basal polarity
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: establishment of epithelial cell apical/basal polarity is consistent with core
      Cdc42 GTPase, cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0051233
    label: spindle midzone
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: spindle midzone is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: UniProt:P60766
      supporting_text: Moves to the central spindle as cells progressed through anaphase to telophase.
- term:
    id: GO:0051489
    label: regulation of filopodium assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: regulation of filopodium assembly is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0051491
    label: positive regulation of filopodium assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: positive regulation of filopodium assembly is consistent with core Cdc42 GTPase,
      cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0051492
    label: regulation of stress fiber assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: The stress-fiber wording is too specific for the available Cdc42 evidence; the
      supported biology is broader actin organization through Cdc42 effector pathways.
    action: MODIFY
    reason: Use a broader actin-organization term rather than treating stress-fiber assembly
      as a core Cdc42 output.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
    proposed_replacement_terms:
    - id: GO:0007015
      label: actin filament organization
- term:
    id: GO:0051496
    label: positive regulation of stress fiber assembly
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: The stress-fiber wording is too specific for the available Cdc42 evidence; the
      supported biology is broader actin organization through Cdc42 effector pathways.
    action: MODIFY
    reason: Use a broader actin-organization term rather than treating stress-fiber assembly
      as a core Cdc42 output.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
    proposed_replacement_terms:
    - id: GO:0007015
      label: actin filament organization
- term:
    id: GO:0051988
    label: regulation of attachment of spindle microtubules to kinetochore
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: regulation of attachment of spindle microtubules to kinetochore is consistent
      with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: UniProt:P60766
      supporting_text: Regulates the bipolar attachment of spindle microtubules to kinetochores
        before chromosome congression in metaphase.
- term:
    id: GO:0061630
    label: ubiquitin protein ligase activity
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: Cdc42 is a small GTPase, not a ubiquitin-protein ligase.
    action: REMOVE
    reason: No direct evidence supports assigning E3 ligase catalytic activity to Cdc42; this
      appears to be an over-transfer from associated pathways or interactors.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0072686
    label: mitotic spindle
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: mitotic spindle is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: UniProt:P60766
      supporting_text: Cytoplasm, cytoskeleton, spindle
- term:
    id: GO:1900026
    label: positive regulation of substrate adhesion-dependent cell spreading
  evidence_type: IEA
  original_reference_id: GO_REF:0000107
  review:
    summary: positive regulation of substrate adhesion-dependent cell spreading is a supported
      context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0015630
    label: microtubule cytoskeleton
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: microtubule cytoskeleton is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0043085
    label: positive regulation of catalytic activity
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of catalytic activity is a broad ancestor for the Cdc42 signaling
      role.
    action: MODIFY
    reason: Cdc42 is best represented as a small GTPase/Rho-family signaling switch.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 belongs to the **Rho family** of small GTPases and acts as a
        **molecular switch** cycling between **GTP-bound (active)** and **GDP-bound (inactive)**
        states
    proposed_replacement_terms:
    - *id001
- term:
    id: GO:0000139
    label: Golgi membrane
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: Golgi membrane is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0002040
    label: sprouting angiogenesis
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: sprouting angiogenesis is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0003924
    label: GTPase activity
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0005525
    label: GTP binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: GTP binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or
      localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0005525
    label: GTP binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: GTP binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or
      localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: cytoplasm is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization
      biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0005813
    label: centrosome
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: centrosome is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization
      biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0006911
    label: phagocytosis, engulfment
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: phagocytosis, engulfment is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0007015
    label: actin filament organization
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: actin filament organization is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0007015
    label: actin filament organization
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: actin filament organization is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0007030
    label: Golgi organization
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: Golgi organization is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0007229
    label: integrin-mediated signaling pathway
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: integrin-mediated signaling pathway is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0010591
    label: regulation of lamellipodium assembly
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: regulation of lamellipodium assembly is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0010592
    label: positive regulation of lamellipodium assembly
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: positive regulation of lamellipodium assembly is consistent with core Cdc42 GTPase,
      cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0016020
    label: membrane
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization
      biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0017119
    label: Golgi transport complex
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: Golgi transport complex is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0019901
    label: protein kinase binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: protein kinase binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0030036
    label: actin cytoskeleton organization
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: actin cytoskeleton organization is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0030141
    label: secretory granule
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: secretory granule is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0030175
    label: filopodium
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: filopodium is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization
      biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0030307
    label: positive regulation of cell growth
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: positive regulation of cell growth is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0030335
    label: positive regulation of cell migration
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: positive regulation of cell migration is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0030496
    label: midbody
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: midbody is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization
      biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0031274
    label: positive regulation of pseudopodium assembly
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: positive regulation of pseudopodium assembly is consistent with core Cdc42 GTPase,
      cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0031333
    label: negative regulation of protein-containing complex assembly
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: negative regulation of protein-containing complex assembly is a supported context-specific
      Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0031435
    label: mitogen-activated protein kinase kinase kinase binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: mitogen-activated protein kinase kinase kinase binding is consistent with core
      Cdc42 GTPase, cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0031996
    label: thioesterase binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: thioesterase binding is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0032427
    label: GBD domain binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: GBD domain binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0032467
    label: positive regulation of cytokinesis
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: positive regulation of cytokinesis is supported for Cdc42, but this is a cell-cycle
      output rather than the small GTPase catalytic role itself.
    action: ACCEPT
    reason: UniProt carries this transferred process annotation, but it is treated as a
      downstream cell-cycle output rather than core GTPase catalysis.
    supported_by:
    - reference_id: UniProt:P60766
      supporting_text: 'GO; GO:0032467; P:positive regulation of cytokinesis; ISS:UniProtKB.'
- term:
    id: GO:0032956
    label: regulation of actin cytoskeleton organization
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: regulation of actin cytoskeleton organization is a supported context-specific
      Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0032991
    label: protein-containing complex
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: protein-containing complex is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0034191
    label: apolipoprotein A-I receptor binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: apolipoprotein A-I receptor binding is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0034329
    label: cell junction assembly
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: cell junction assembly is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0036464
    label: cytoplasmic ribonucleoprotein granule
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: cytoplasmic ribonucleoprotein granule is retained as a non-core Cdc42-associated
      annotation.
    action: KEEP_AS_NON_CORE
    reason: The annotation is plausible as a downstream or compartment-specific consequence
      of Cdc42 signaling rather than the core GTPase switch activity.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0038189
    label: neuropilin signaling pathway
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: neuropilin signaling pathway is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0042802
    label: identical protein binding
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: identical protein binding is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0043005
    label: neuron projection
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: neuron projection is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0043025
    label: neuronal cell body
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: neuronal cell body is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0043525
    label: positive regulation of neuron apoptotic process
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of neuron apoptotic process is a supported context-specific
      Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0044788
    label: host-mediated perturbation of viral process
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: host-mediated perturbation of viral process is a supported context-specific Cdc42
      output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0045198
    label: establishment of epithelial cell apical/basal polarity
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: establishment of epithelial cell apical/basal polarity is consistent with core
      Cdc42 GTPase, cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0045740
    label: positive regulation of DNA replication
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of DNA replication is a supported context-specific Cdc42
      output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0046330
    label: positive regulation of JNK cascade
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: The current local evidence supports context-specific Cdc42/p38 MAPK signaling,
      but does not establish a specific JNK cascade annotation.
    action: UNDECIDED
    reason: Retaining this JNK-specific automated transfer requires direct term-specific evidence.
- term:
    id: GO:0051233
    label: spindle midzone
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: spindle midzone is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: UniProt:P60766
      supporting_text: Moves to the central spindle as cells progressed through anaphase to telophase.
- term:
    id: GO:0051489
    label: regulation of filopodium assembly
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: regulation of filopodium assembly is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0051491
    label: positive regulation of filopodium assembly
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: positive regulation of filopodium assembly is consistent with core Cdc42 GTPase,
      cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0051492
    label: regulation of stress fiber assembly
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: The stress-fiber wording is too specific for the available Cdc42 evidence; the
      supported biology is broader actin organization through Cdc42 effector pathways.
    action: MODIFY
    reason: Use a broader actin-organization term rather than treating stress-fiber assembly
      as a core Cdc42 output.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
    proposed_replacement_terms:
    - id: GO:0007015
      label: actin filament organization
- term:
    id: GO:0051496
    label: positive regulation of stress fiber assembly
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: The stress-fiber wording is too specific for the available Cdc42 evidence; the
      supported biology is broader actin organization through Cdc42 effector pathways.
    action: MODIFY
    reason: Use a broader actin-organization term rather than treating stress-fiber assembly
      as a core Cdc42 output.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
    proposed_replacement_terms:
    - id: GO:0007015
      label: actin filament organization
- term:
    id: GO:0051683
    label: establishment of Golgi localization
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: establishment of Golgi localization is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0051835
    label: positive regulation of synapse structural plasticity
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of synapse structural plasticity is a supported context-specific
      Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0051988
    label: regulation of attachment of spindle microtubules to kinetochore
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: regulation of attachment of spindle microtubules to kinetochore is consistent
      with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: UniProt:P60766
      supporting_text: Regulates the bipolar attachment of spindle microtubules to kinetochores
        before chromosome congression in metaphase.
- term:
    id: GO:0061630
    label: ubiquitin protein ligase activity
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: Cdc42 is a small GTPase, not a ubiquitin-protein ligase.
    action: REMOVE
    reason: No direct evidence supports assigning E3 ligase catalytic activity to Cdc42; this
      appears to be an over-transfer from associated pathways or interactors.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0072384
    label: organelle transport along microtubule
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: organelle transport along microtubule is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0072686
    label: mitotic spindle
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: mitotic spindle is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: UniProt:P60766
      supporting_text: Cytoplasm, cytoskeleton, spindle
- term:
    id: GO:0090135
    label: actin filament branching
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: actin filament branching is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0090316
    label: positive regulation of intracellular protein transport
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: positive regulation of intracellular protein transport is a supported context-specific
      Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0098685
    label: Schaffer collateral - CA1 synapse
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: Schaffer collateral - CA1 synapse is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0099159
    label: regulation of modification of postsynaptic structure
  evidence_type: ISO
  original_reference_id: GO_REF:0000096
  review:
    summary: regulation of modification of postsynaptic structure is a supported context-specific
      Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:1900026
    label: positive regulation of substrate adhesion-dependent cell spreading
  evidence_type: ISO
  original_reference_id: GO_REF:0000119
  review:
    summary: positive regulation of substrate adhesion-dependent cell spreading is a supported
      context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:24076653
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:37140151
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0098794
    label: postsynapse
  evidence_type: IDA
  original_reference_id: PMID:33531495
  review:
    summary: postsynapse is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0098794
    label: postsynapse
  evidence_type: IMP
  original_reference_id: PMID:33531495
  review:
    summary: postsynapse is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0098978
    label: glutamatergic synapse
  evidence_type: IDA
  original_reference_id: PMID:25851601
  review:
    summary: glutamatergic synapse is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0098978
    label: glutamatergic synapse
  evidence_type: IMP
  original_reference_id: PMID:25851601
  review:
    summary: glutamatergic synapse is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0098978
    label: glutamatergic synapse
  evidence_type: IDA
  original_reference_id: PMID:33531495
  review:
    summary: glutamatergic synapse is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0098978
    label: glutamatergic synapse
  evidence_type: IMP
  original_reference_id: PMID:33531495
  review:
    summary: glutamatergic synapse is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0099175
    label: regulation of postsynapse organization
  evidence_type: IDA
  original_reference_id: PMID:25851601
  review:
    summary: regulation of postsynapse organization is a supported context-specific Cdc42
      output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0099175
    label: regulation of postsynapse organization
  evidence_type: IMP
  original_reference_id: PMID:25851601
  review:
    summary: regulation of postsynapse organization is a supported context-specific Cdc42
      output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0003015
    label: heart process
  evidence_type: IMP
  original_reference_id: PMID:25197047
  review:
    summary: heart process is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0003015
    label: heart process
  evidence_type: IMP
  original_reference_id: PMID:31219639
  review:
    summary: heart process is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0003253
    label: cardiac neural crest cell migration involved in outflow tract morphogenesis
  evidence_type: IMP
  original_reference_id: PMID:31219639
  review:
    summary: cardiac neural crest cell migration involved in outflow tract morphogenesis is
      a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0006468
    label: protein phosphorylation
  evidence_type: IMP
  original_reference_id: PMID:31219639
  review:
    summary: Cdc42 does not itself catalyze protein phosphorylation.
    action: MODIFY
    reason: The evidence supports upstream small-GTPase signaling to kinase effectors such
      as PAK rather than direct kinase activity by Cdc42.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
    proposed_replacement_terms:
    - *id001
- term:
    id: GO:0031252
    label: cell leading edge
  evidence_type: IDA
  original_reference_id: PMID:31219639
  review:
    summary: cell leading edge is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0035050
    label: embryonic heart tube development
  evidence_type: IMP
  original_reference_id: PMID:31219639
  review:
    summary: embryonic heart tube development is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0086100
    label: endothelin receptor signaling pathway
  evidence_type: IMP
  original_reference_id: PMID:31219639
  review:
    summary: endothelin receptor signaling pathway is retained as a non-core Cdc42-associated
      annotation.
    action: KEEP_AS_NON_CORE
    reason: The annotation is plausible as a downstream or compartment-specific consequence
      of Cdc42 signaling rather than the core GTPase switch activity.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:15710388
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:18604197
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:21828338
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:8464478
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0003924
    label: GTPase activity
  evidence_type: ISO
  original_reference_id: PMID:27917469
  review:
    summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0048549
    label: positive regulation of pinocytosis
  evidence_type: IMP
  original_reference_id: PMID:24792215
  review:
    summary: positive regulation of pinocytosis is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0045335
    label: phagocytic vesicle
  evidence_type: IDA
  original_reference_id: PMID:19144319
  review:
    summary: phagocytic vesicle is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0071346
    label: cellular response to type II interferon
  evidence_type: IDA
  original_reference_id: PMID:19144319
  review:
    summary: cellular response to type II interferon is a supported context-specific Cdc42
      output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0006911
    label: phagocytosis, engulfment
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  review:
    summary: phagocytosis, engulfment is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0007015
    label: actin filament organization
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  review:
    summary: actin filament organization is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0034329
    label: cell junction assembly
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  review:
    summary: cell junction assembly is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0045198
    label: establishment of epithelial cell apical/basal polarity
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  review:
    summary: establishment of epithelial cell apical/basal polarity is consistent with core
      Cdc42 GTPase, cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0051491
    label: positive regulation of filopodium assembly
  evidence_type: IMP
  original_reference_id: PMID:22494997
  review:
    summary: positive regulation of filopodium assembly is consistent with core Cdc42 GTPase,
      cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0060997
    label: dendritic spine morphogenesis
  evidence_type: IMP
  original_reference_id: PMID:25851601
  review:
    summary: dendritic spine morphogenesis is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0003924
    label: GTPase activity
  evidence_type: IDA
  original_reference_id: PMID:18838382
  review:
    summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0003924
    label: GTPase activity
  evidence_type: IGI
  original_reference_id: PMID:18838382
  review:
    summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:18838382
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0000322
    label: storage vacuole
  evidence_type: IDA
  original_reference_id: PMID:21828338
  review:
    summary: storage vacuole is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005829
    label: cytosol
  evidence_type: IDA
  original_reference_id: PMID:21828338
  review:
    summary: cytosol is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization
      biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: IDA
  original_reference_id: PMID:21828338
  review:
    summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: IDA
  original_reference_id: PMID:25217619
  review:
    summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-3928643
  review:
    summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-442572
  review:
    summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-448947
  review:
    summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0005886
    label: plasma membrane
  evidence_type: TAS
  original_reference_id: Reactome:R-MMU-5665684
  review:
    summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0006897
    label: endocytosis
  evidence_type: IMP
  original_reference_id: PMID:24792215
  review:
    summary: PMID:24792215 directly links Cdc42 cycling to actin-dependent fluid-phase
      endocytosis.
    action: KEEP_AS_NON_CORE
    reason: The process is supported for Cdc42-mediated actin cycling, but it remains a
      context-specific trafficking output rather than the core small-GTPase molecular function.
    supported_by:
    - reference_id: PMID:24792215
      supporting_text: The small GTPase Cdc42 regulates sequential synthesis and break down of actin allowing fluid-phase uptake to occur
- term:
    id: GO:0043209
    label: myelin sheath
  evidence_type: HDA
  original_reference_id: PMID:17634366
  review:
    summary: myelin sheath is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0031256
    label: leading edge membrane
  evidence_type: IDA
  original_reference_id: PMID:22461490
  review:
    summary: leading edge membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0036336
    label: dendritic cell migration
  evidence_type: IGI
  original_reference_id: PMID:22461490
  review:
    summary: dendritic cell migration is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005525
    label: GTP binding
  evidence_type: ISO
  original_reference_id: PMID:23620790
  review:
    summary: GTP binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or
      localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0005911
    label: cell-cell junction
  evidence_type: IDA
  original_reference_id: PMID:23793062
  review:
    summary: cell-cell junction is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0016020
    label: membrane
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  review:
    summary: membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization
      biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:1900026
    label: positive regulation of substrate adhesion-dependent cell spreading
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  review:
    summary: positive regulation of substrate adhesion-dependent cell spreading is a supported
      context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0060501
    label: positive regulation of epithelial cell proliferation involved in lung morphogenesis
  evidence_type: IMP
  original_reference_id: PMID:23219958
  review:
    summary: positive regulation of epithelial cell proliferation involved in lung morphogenesis
      is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:10954424
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:11035016
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0003161
    label: cardiac conduction system development
  evidence_type: IGI
  original_reference_id: PMID:21690310
  review:
    summary: cardiac conduction system development is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0060047
    label: heart contraction
  evidence_type: IGI
  original_reference_id: PMID:21690310
  review:
    summary: heart contraction is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0019901
    label: protein kinase binding
  evidence_type: IPI
  original_reference_id: PMID:21048939
  review:
    summary: protein kinase binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:17000758
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0030496
    label: midbody
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  review:
    summary: midbody is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization
      biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0032467
    label: positive regulation of cytokinesis
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  review:
    summary: positive regulation of cytokinesis is supported for Cdc42, but this is a cell-cycle
      output rather than the small GTPase catalytic role itself.
    action: ACCEPT
    reason: UniProt carries this transferred process annotation, but it is treated as a
      downstream cell-cycle output rather than core GTPase catalysis.
    supported_by:
    - reference_id: UniProt:P60766
      supporting_text: 'GO; GO:0032467; P:positive regulation of cytokinesis; ISS:UniProtKB.'
- term:
    id: GO:0051233
    label: spindle midzone
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  review:
    summary: spindle midzone is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: UniProt:P60766
      supporting_text: Moves to the central spindle as cells progressed through anaphase to telophase.
- term:
    id: GO:0051489
    label: regulation of filopodium assembly
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  review:
    summary: regulation of filopodium assembly is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0051988
    label: regulation of attachment of spindle microtubules to kinetochore
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  review:
    summary: regulation of attachment of spindle microtubules to kinetochore is consistent
      with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: UniProt:P60766
      supporting_text: Regulates the bipolar attachment of spindle microtubules to kinetochores
        before chromosome congression in metaphase.
- term:
    id: GO:0072686
    label: mitotic spindle
  evidence_type: ISS
  original_reference_id: GO_REF:0000024
  review:
    summary: mitotic spindle is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: UniProt:P60766
      supporting_text: Cytoplasm, cytoskeleton, spindle
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IDA
  original_reference_id: PMID:19796622
  review:
    summary: cytoplasm is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization
      biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0071944
    label: cell periphery
  evidence_type: IDA
  original_reference_id: PMID:19796622
  review:
    summary: cell periphery is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0005737
    label: cytoplasm
  evidence_type: IDA
  original_reference_id: PMID:15775979
  review:
    summary: cytoplasm is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization
      biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal
        prenyl group**
- term:
    id: GO:0043410
    label: positive regulation of MAPK cascade
  evidence_type: IDA
  original_reference_id: PMID:19244314
  review:
    summary: positive regulation of MAPK cascade is supported in a Cdo/JLP/Bnip-2 neuronal
      differentiation context, but it is not Cdc42's core molecular role.
    action: KEEP_AS_NON_CORE
    reason: The cited evidence supports a context-specific Cdc42/p38 MAPK signaling output.
    supported_by:
    - reference_id: PMID:19244314
      supporting_text: These molecules regulate Cdc42 and p38MAPK activities, which increase
        in a Cdo-dependent manner during neuronal differentiation of C17.2 cells and retinoic
        acid-treated P19 cells.
- term:
    id: GO:0003924
    label: GTPase activity
  evidence_type: ISA
  original_reference_id: PMID:12637522
  review:
    summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0007097
    label: nuclear migration
  evidence_type: IMP
  original_reference_id: PMID:16892058
  review:
    summary: nuclear migration is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0008104
    label: intracellular protein localization
  evidence_type: IMP
  original_reference_id: PMID:16621792
  review:
    summary: intracellular protein localization is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0034332
    label: adherens junction organization
  evidence_type: IMP
  original_reference_id: PMID:16892058
  review:
    summary: adherens junction organization is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0035088
    label: establishment or maintenance of apical/basal cell polarity
  evidence_type: IMP
  original_reference_id: PMID:16892058
  review:
    summary: establishment or maintenance of apical/basal cell polarity is consistent with
      core Cdc42 GTPase, cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0051647
    label: nucleus localization
  evidence_type: IMP
  original_reference_id: PMID:16892058
  review:
    summary: nucleus localization is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0007088
    label: regulation of mitotic nuclear division
  evidence_type: IMP
  original_reference_id: PMID:16892058
  review:
    summary: regulation of mitotic nuclear division is a supported context-specific Cdc42
      output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0045177
    label: apical part of cell
  evidence_type: IDA
  original_reference_id: PMID:16892058
  review:
    summary: apical part of cell is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0048664
    label: neuron fate determination
  evidence_type: IMP
  original_reference_id: PMID:16892058
  review:
    summary: neuron fate determination is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0007163
    label: establishment or maintenance of cell polarity
  evidence_type: TAS
  original_reference_id: PMID:10954424
  review:
    summary: establishment or maintenance of cell polarity is consistent with core Cdc42 GTPase,
      cytoskeletal, polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0016197
    label: endosomal transport
  evidence_type: TAS
  original_reference_id: PMID:10954424
  review:
    summary: endosomal transport is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0098609
    label: cell-cell adhesion
  evidence_type: TAS
  original_reference_id: PMID:10954424
  review:
    summary: cell-cell adhesion is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0007097
    label: nuclear migration
  evidence_type: IMP
  original_reference_id: PMID:15882626
  review:
    summary: nuclear migration is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:15996550
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0042995
    label: cell projection
  evidence_type: IDA
  original_reference_id: PMID:15728722
  review:
    summary: cell projection is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0051897
    label: positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
  evidence_type: IMP
  original_reference_id: PMID:15728722
  review:
    summary: positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal
      transduction is a supported context-specific Cdc42 output.
    action: KEEP_AS_NON_CORE
    reason: Cdc42 controls broad cellular architecture and developmental programs through
      actin, polarity, trafficking, and kinase-effector pathways, but this term is not the
      core molecular function.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:16052498
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0005515
    label: protein binding
  evidence_type: IPI
  original_reference_id: PMID:12773384
  review:
    summary: protein binding records a physical association but is too generic to describe
      the gene product function.
    action: MARK_AS_OVER_ANNOTATED
    reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone
      protein binding function; more informative molecular and pathway terms capture the biology.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: In its **GTP-bound state**, CDC42 binds effector proteins that drive
        downstream cytoskeletal and polarity responses
- term:
    id: GO:0003924
    label: GTPase activity
  evidence_type: IDA
  original_reference_id: PMID:15249579
  review:
    summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0030742
    label: GTP-dependent protein binding
  evidence_type: IPI
  original_reference_id: PMID:10954424
  review:
    summary: GTP-dependent protein binding is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0003924
    label: GTPase activity
  evidence_type: IDA
  original_reference_id: PMID:10699171
  review:
    summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0046847
    label: filopodium assembly
  evidence_type: IDA
  original_reference_id: PMID:10699171
  review:
    summary: filopodium assembly is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
- term:
    id: GO:0003924
    label: GTPase activity
  evidence_type: TAS
  original_reference_id: PMID:10490598
  review:
    summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity,
      or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate
        is **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- term:
    id: GO:0007266
    label: Rho protein signal transduction
  evidence_type: TAS
  original_reference_id: PMID:10490598
  review:
    summary: Rho protein signal transduction is consistent with core Cdc42 GTPase, cytoskeletal,
      polarity, or localization biology.
    action: ACCEPT
    reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane
      localization, or immediate actin/polarity outputs.
    supported_by:
    - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
      supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
        nucleation-promoting factors**.
core_functions:
- description: Cycles between GDP- and GTP-bound states and hydrolyzes GTP to act as a Rho-family
    molecular switch.
  molecular_function: *id002
  directly_involved_in:
  - id: GO:0007266
    label: Rho protein signal transduction
  locations:
  - id: GO:0005886
    label: plasma membrane
  - id: GO:0005829
    label: cytosol
  supported_by:
  - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
    supporting_text: CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is
      **GTP**, which it binds and hydrolyzes to **GDP + Pi**
- description: Recruits active-state effectors including WASP/N-WASP, PAK, and polarity complexes
    to organize actin, filopodia, cell polarity, and migration.
  molecular_function:
    id: GO:0030742
    label: GTP-dependent protein binding
  directly_involved_in:
  - id: GO:0007015
    label: actin filament organization
  - id: GO:0030010
    label: establishment of cell polarity
  - id: GO:0046847
    label: filopodium assembly
  locations:
  - id: GO:0005886
    label: plasma membrane
  - id: GO:0031256
    label: leading edge membrane
  supported_by:
  - reference_id: file:mouse/Cdc42/Cdc42-deep-research-falcon.md
    supporting_text: CDC42 is directly linked to actin nucleation pathways through **WASP-family
      nucleation-promoting factors**.
proposed_new_terms: []
suggested_questions:
- question: Which mouse Cdc42 annotations should be isoform-qualified for prenylated versus
    palmitoylated Cdc42 products?
- question: Which neuronal/synaptic Cdc42 terms are directly supported for the brain-enriched
    isoform rather than inferred from general Rho-family biology?
suggested_experiments:
- description: Use isoform-specific knock-in tags and rescue alleles to measure Cdc42 effector
    recruitment at leading edge, Golgi/endosomal, midbody, and neuronal compartments.
  hypothesis: Cdc42 pleiotropy is primarily explained by isoform- and lipidation-dependent
    localization rather than different intrinsic GTPase biochemistry.
  experiment_type: Live-cell imaging and isoform-specific rescue