Cdc42 is a conserved Rho-family small GTPase that functions as a GDP/GTP-regulated molecular switch. Active GTP-bound Cdc42 binds effectors such as WASP/N-WASP, PAK, and Par6/aPKC polarity complexes to organize cortical actin, filopodia, cell polarity, junctions, migration, endocytosis, cytokinesis, and spindle-related processes. Its outputs depend strongly on membrane targeting and isoform-specific C-terminal lipidation: the broadly expressed prenylated isoform acts at leading-edge/cortical membranes, while a brain-enriched palmitoylated isoform contributes to intracellular membrane and neuronal contexts.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003924 GTPase activity | IBA GO_REF:0000033 | ACCEPT | Summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0005525 GTP binding | IBA GO_REF:0000033 | ACCEPT | Summary: GTP binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0007165 signal transduction | IBA GO_REF:0000033 | MODIFY | Summary: signal transduction is a broad ancestor for the Cdc42 signaling role. Reason: Cdc42 is best represented as a small GTPase/Rho-family signaling switch. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: GRANULARITY MISMATCH Sources checked: PANTHER:PTN000632671 SUPPORTS TRANSFER The signal-transduction IBD sits at this node, whose seeds run across Candida, Drosophila, mouse, rat and Arabidopsis members. Participation in intracellular signalling is thus an ancient, broadly supported property of the group and descends soundly to mouse Cdc42; the only fault is that the term is the top-level signal-transduction parent, which says nothing about the mechanism. MGI:MGI:106211 Β· mouse Cdc42 (this gene) SUPPORTS TRANSFER The target's own IBD seed. Mouse Cdc42 carries its own experimental annotation to Rho protein signal transduction (GO:0007266, TAS), a descendant of the proposed replacement term, and that evidence is one of the descendants the PAINT curator used when placing this node. It marks experimental grounding on the target itself and shows the propagated term simply sits higher in the same lineage. CGD:CAL0000192659 SUPPORTS TRANSFER Candida seed cited by identifier because the local index does not resolve it; part of the fungal coverage that dates the signalling role well before vertebrates. FB:FBgn0010333 SUPPORTS TRANSFER Drosophila seed, cited by identifier only; part of the invertebrate coverage of this node. Proposed replacements: small GTPase-mediated signal transduction Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 belongs to the **Rho family** of small GTPases and acts as a **molecular switch** cycling between **GTP-bound (active)** and **GDP-bound (inactive)** states |
| GO:0005886 plasma membrane | IBA GO_REF:0000033 | ACCEPT | Summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0007015 actin filament organization | IBA GO_REF:0000033 | ACCEPT | Summary: actin filament organization is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0030010 establishment of cell polarity | IBA GO_REF:0000033 | ACCEPT | Summary: establishment of cell polarity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42b supports **N-WASP-mediated endocytosis** from intracellular compartments. |
| GO:0006897 endocytosis | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: endocytosis is a supported Cdc42 cellular output, but it is not the core small GTPase catalytic function. Reason: Cdc42 GTP-bound effector signaling can influence trafficking and membrane dynamics, but this process should be treated as context-specific. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0019901 protein kinase binding | IBA GO_REF:0000033 | ACCEPT | Summary: protein kinase binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0051489 regulation of filopodium assembly | IBA GO_REF:0000033 | ACCEPT | Summary: regulation of filopodium assembly is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0000166 nucleotide binding | IEA GO_REF:0000043 | MODIFY | Summary: Nucleotide binding is too broad for Cdc42. Reason: Cdc42 specifically binds guanine nucleotides and functions through GTP/GDP cycling. Proposed replacements: GTP binding Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0003924 GTPase activity | IEA GO_REF:0000120 | ACCEPT | Summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0003925 G protein activity | IEA GO_REF:0000003 | ACCEPT | Summary: G protein activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0005525 GTP binding | IEA GO_REF:0000120 | ACCEPT | Summary: GTP binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0005737 cytoplasm | IEA GO_REF:0000120 | ACCEPT | Summary: cytoplasm is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0005813 centrosome | IEA GO_REF:0000120 | ACCEPT | Summary: centrosome is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0005819 spindle | IEA GO_REF:0000044 | REMOVE | Summary: This automated generic cellular-component transfer is too broad relative to the narrower Cdc42 localization annotations retained elsewhere. Reason: The local evidence does not justify keeping this broad automated component row as a separate accepted annotation; it was previously supported only by unrelated actin-effector biology. |
| GO:0005886 plasma membrane | IEA GO_REF:0000120 | ACCEPT | Summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0007264 small GTPase-mediated signal transduction | IEA GO_REF:0000002 | ACCEPT | Summary: small GTPase-mediated signal transduction is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0007399 nervous system development | IEA GO_REF:0000043 | KEEP AS NON CORE | Summary: nervous system development is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0009653 anatomical structure morphogenesis | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: anatomical structure morphogenesis is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0016787 hydrolase activity | IEA GO_REF:0000043 | MODIFY | Summary: Hydrolase activity is too broad for Cdc42. Reason: The relevant hydrolytic activity is GTPase activity in a Rho-family molecular switch. Proposed replacements: GTPase activity Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0030154 cell differentiation | IEA GO_REF:0000043 | KEEP AS NON CORE | Summary: cell differentiation is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0030425 dendrite | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: dendrite is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0030496 midbody | IEA GO_REF:0000120 | ACCEPT | Summary: midbody is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0031258 lamellipodium membrane | IEA GO_REF:0000044 | ACCEPT | Summary: lamellipodium membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0051130 positive regulation of cellular component organization | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: positive regulation of cellular component organization is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0071944 cell periphery | IEA GO_REF:0000117 | ACCEPT | Summary: cell periphery is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0005515 protein binding | IPI PMID:12123608 Plexin-B1 directly interacts with PDZ-RhoGEF/LARG to regulat... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:15537656 A direct interaction between Cdc42 and vesicle-associated me... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:18325335 Affixin activates Rac1 via betaPIX in C2C12 myoblast. | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:18541910 Bves directly interacts with GEFT, and controls cell shape a... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:18854161 ACF7 regulates cytoskeletal-focal adhesion dynamics and migr... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:19893577 The adenomatous polyposis coli-associated exchange factors A... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:23706742 RAS and RHO families of GTPases directly regulate distinct p... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0006911 phagocytosis, engulfment | IEA GO_REF:0000107 | ACCEPT | Summary: phagocytosis, engulfment is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0007015 actin filament organization | IEA GO_REF:0000107 | ACCEPT | Summary: actin filament organization is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0007229 integrin-mediated signaling pathway | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: integrin-mediated signaling pathway is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0010591 regulation of lamellipodium assembly | IEA GO_REF:0000107 | ACCEPT | Summary: regulation of lamellipodium assembly is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0010592 positive regulation of lamellipodium assembly | IEA GO_REF:0000107 | ACCEPT | Summary: positive regulation of lamellipodium assembly is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0015630 microtubule cytoskeleton | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: microtubule cytoskeleton is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0016020 membrane | IEA GO_REF:0000107 | ACCEPT | Summary: membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0017119 Golgi transport complex | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Golgi transport complex is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0019901 protein kinase binding | IEA GO_REF:0000120 | ACCEPT | Summary: protein kinase binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0030036 actin cytoskeleton organization | IEA GO_REF:0000107 | ACCEPT | Summary: actin cytoskeleton organization is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0030175 filopodium | IEA GO_REF:0000107 | ACCEPT | Summary: filopodium is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0030307 positive regulation of cell growth | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: positive regulation of cell growth is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0030335 positive regulation of cell migration | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: positive regulation of cell migration is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0031274 positive regulation of pseudopodium assembly | IEA GO_REF:0000107 | ACCEPT | Summary: positive regulation of pseudopodium assembly is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0031333 negative regulation of protein-containing complex assembly | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: negative regulation of protein-containing complex assembly is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0031996 thioesterase binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: thioesterase binding is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0032427 GBD domain binding | IEA GO_REF:0000107 | ACCEPT | Summary: GBD domain binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0032467 positive regulation of cytokinesis | IEA GO_REF:0000107 | ACCEPT | Summary: positive regulation of cytokinesis is supported for Cdc42, but this is a cell-cycle output rather than the small GTPase catalytic role itself. Reason: UniProt carries this transferred process annotation, but it is treated as a downstream cell-cycle output rather than core GTPase catalysis. Supporting Evidence: UniProt:P60766 GO; GO:0032467; P:positive regulation of cytokinesis; ISS:UniProtKB. |
| GO:0032956 regulation of actin cytoskeleton organization | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: regulation of actin cytoskeleton organization is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0032991 protein-containing complex | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: protein-containing complex is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0034191 apolipoprotein A-I receptor binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: apolipoprotein A-I receptor binding is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0034329 cell junction assembly | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: cell junction assembly is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0036464 cytoplasmic ribonucleoprotein granule | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: cytoplasmic ribonucleoprotein granule is retained as a non-core Cdc42-associated annotation. Reason: The annotation is plausible as a downstream or compartment-specific consequence of Cdc42 signaling rather than the core GTPase switch activity. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0038189 neuropilin signaling pathway | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: neuropilin signaling pathway is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0042802 identical protein binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: identical protein binding is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0043005 neuron projection | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: neuron projection is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0043025 neuronal cell body | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: neuronal cell body is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0044788 host-mediated perturbation of viral process | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: host-mediated perturbation of viral process is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0045198 establishment of epithelial cell apical/basal polarity | IEA GO_REF:0000107 | ACCEPT | Summary: establishment of epithelial cell apical/basal polarity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0051233 spindle midzone | IEA GO_REF:0000107 | ACCEPT | Summary: spindle midzone is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: UniProt:P60766 Moves to the central spindle as cells progressed through anaphase to telophase. |
| GO:0051489 regulation of filopodium assembly | IEA GO_REF:0000107 | ACCEPT | Summary: regulation of filopodium assembly is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0051491 positive regulation of filopodium assembly | IEA GO_REF:0000107 | ACCEPT | Summary: positive regulation of filopodium assembly is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0051492 regulation of stress fiber assembly | IEA GO_REF:0000107 | MODIFY | Summary: The stress-fiber wording is too specific for the available Cdc42 evidence; the supported biology is broader actin organization through Cdc42 effector pathways. Reason: Use a broader actin-organization term rather than treating stress-fiber assembly as a core Cdc42 output. Proposed replacements: actin filament organization Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0051496 positive regulation of stress fiber assembly | IEA GO_REF:0000107 | MODIFY | Summary: The stress-fiber wording is too specific for the available Cdc42 evidence; the supported biology is broader actin organization through Cdc42 effector pathways. Reason: Use a broader actin-organization term rather than treating stress-fiber assembly as a core Cdc42 output. Proposed replacements: actin filament organization Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0051988 regulation of attachment of spindle microtubules to kinetochore | IEA GO_REF:0000107 | ACCEPT | Summary: regulation of attachment of spindle microtubules to kinetochore is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: UniProt:P60766 Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. |
| GO:0061630 ubiquitin protein ligase activity | IEA GO_REF:0000107 | REMOVE | Summary: Cdc42 is a small GTPase, not a ubiquitin-protein ligase. Reason: No direct evidence supports assigning E3 ligase catalytic activity to Cdc42; this appears to be an over-transfer from associated pathways or interactors. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0072686 mitotic spindle | IEA GO_REF:0000107 | ACCEPT | Summary: mitotic spindle is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: UniProt:P60766 Cytoplasm, cytoskeleton, spindle |
| GO:1900026 positive regulation of substrate adhesion-dependent cell spreading | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: positive regulation of substrate adhesion-dependent cell spreading is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0015630 microtubule cytoskeleton | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: microtubule cytoskeleton is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0043085 positive regulation of catalytic activity | ISO GO_REF:0000096 | MODIFY | Summary: positive regulation of catalytic activity is a broad ancestor for the Cdc42 signaling role. Reason: Cdc42 is best represented as a small GTPase/Rho-family signaling switch. Proposed replacements: small GTPase-mediated signal transduction Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 belongs to the **Rho family** of small GTPases and acts as a **molecular switch** cycling between **GTP-bound (active)** and **GDP-bound (inactive)** states |
| GO:0000139 Golgi membrane | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: Golgi membrane is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0002040 sprouting angiogenesis | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: sprouting angiogenesis is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0003924 GTPase activity | ISO GO_REF:0000119 | ACCEPT | Summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0005525 GTP binding | ISO GO_REF:0000096 | ACCEPT | Summary: GTP binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0005525 GTP binding | ISO GO_REF:0000119 | ACCEPT | Summary: GTP binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0005737 cytoplasm | ISO GO_REF:0000119 | ACCEPT | Summary: cytoplasm is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0005813 centrosome | ISO GO_REF:0000119 | ACCEPT | Summary: centrosome is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0005886 plasma membrane | ISO GO_REF:0000096 | ACCEPT | Summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0005886 plasma membrane | ISO GO_REF:0000119 | ACCEPT | Summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0006911 phagocytosis, engulfment | ISO GO_REF:0000119 | ACCEPT | Summary: phagocytosis, engulfment is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0007015 actin filament organization | ISO GO_REF:0000096 | ACCEPT | Summary: actin filament organization is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0007015 actin filament organization | ISO GO_REF:0000119 | ACCEPT | Summary: actin filament organization is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0007030 Golgi organization | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: Golgi organization is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0007229 integrin-mediated signaling pathway | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: integrin-mediated signaling pathway is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0010591 regulation of lamellipodium assembly | ISO GO_REF:0000119 | ACCEPT | Summary: regulation of lamellipodium assembly is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0010592 positive regulation of lamellipodium assembly | ISO GO_REF:0000119 | ACCEPT | Summary: positive regulation of lamellipodium assembly is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0016020 membrane | ISO GO_REF:0000119 | ACCEPT | Summary: membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0017119 Golgi transport complex | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: Golgi transport complex is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0019901 protein kinase binding | ISO GO_REF:0000119 | ACCEPT | Summary: protein kinase binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0030036 actin cytoskeleton organization | ISO GO_REF:0000119 | ACCEPT | Summary: actin cytoskeleton organization is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0030141 secretory granule | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: secretory granule is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0030175 filopodium | ISO GO_REF:0000119 | ACCEPT | Summary: filopodium is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0030307 positive regulation of cell growth | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: positive regulation of cell growth is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0030335 positive regulation of cell migration | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: positive regulation of cell migration is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0030496 midbody | ISO GO_REF:0000119 | ACCEPT | Summary: midbody is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0031274 positive regulation of pseudopodium assembly | ISO GO_REF:0000119 | ACCEPT | Summary: positive regulation of pseudopodium assembly is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0031333 negative regulation of protein-containing complex assembly | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: negative regulation of protein-containing complex assembly is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0031435 mitogen-activated protein kinase kinase kinase binding | ISO GO_REF:0000096 | ACCEPT | Summary: mitogen-activated protein kinase kinase kinase binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0031996 thioesterase binding | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: thioesterase binding is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0032427 GBD domain binding | ISO GO_REF:0000119 | ACCEPT | Summary: GBD domain binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0032467 positive regulation of cytokinesis | ISO GO_REF:0000119 | ACCEPT | Summary: positive regulation of cytokinesis is supported for Cdc42, but this is a cell-cycle output rather than the small GTPase catalytic role itself. Reason: UniProt carries this transferred process annotation, but it is treated as a downstream cell-cycle output rather than core GTPase catalysis. Supporting Evidence: UniProt:P60766 GO; GO:0032467; P:positive regulation of cytokinesis; ISS:UniProtKB. |
| GO:0032956 regulation of actin cytoskeleton organization | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: regulation of actin cytoskeleton organization is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0032991 protein-containing complex | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: protein-containing complex is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0034191 apolipoprotein A-I receptor binding | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: apolipoprotein A-I receptor binding is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0034329 cell junction assembly | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: cell junction assembly is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0036464 cytoplasmic ribonucleoprotein granule | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: cytoplasmic ribonucleoprotein granule is retained as a non-core Cdc42-associated annotation. Reason: The annotation is plausible as a downstream or compartment-specific consequence of Cdc42 signaling rather than the core GTPase switch activity. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0038189 neuropilin signaling pathway | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: neuropilin signaling pathway is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0042802 identical protein binding | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: identical protein binding is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0043005 neuron projection | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: neuron projection is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0043025 neuronal cell body | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: neuronal cell body is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0043525 positive regulation of neuron apoptotic process | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: positive regulation of neuron apoptotic process is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0044788 host-mediated perturbation of viral process | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: host-mediated perturbation of viral process is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0045198 establishment of epithelial cell apical/basal polarity | ISO GO_REF:0000119 | ACCEPT | Summary: establishment of epithelial cell apical/basal polarity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0045740 positive regulation of DNA replication | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: positive regulation of DNA replication is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0046330 positive regulation of JNK cascade | ISO GO_REF:0000096 | UNDECIDED | Summary: The current local evidence supports context-specific Cdc42/p38 MAPK signaling, but does not establish a specific JNK cascade annotation. Reason: Retaining this JNK-specific automated transfer requires direct term-specific evidence. |
| GO:0051233 spindle midzone | ISO GO_REF:0000119 | ACCEPT | Summary: spindle midzone is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: UniProt:P60766 Moves to the central spindle as cells progressed through anaphase to telophase. |
| GO:0051489 regulation of filopodium assembly | ISO GO_REF:0000119 | ACCEPT | Summary: regulation of filopodium assembly is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0051491 positive regulation of filopodium assembly | ISO GO_REF:0000119 | ACCEPT | Summary: positive regulation of filopodium assembly is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0051492 regulation of stress fiber assembly | ISO GO_REF:0000119 | MODIFY | Summary: The stress-fiber wording is too specific for the available Cdc42 evidence; the supported biology is broader actin organization through Cdc42 effector pathways. Reason: Use a broader actin-organization term rather than treating stress-fiber assembly as a core Cdc42 output. Proposed replacements: actin filament organization Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0051496 positive regulation of stress fiber assembly | ISO GO_REF:0000119 | MODIFY | Summary: The stress-fiber wording is too specific for the available Cdc42 evidence; the supported biology is broader actin organization through Cdc42 effector pathways. Reason: Use a broader actin-organization term rather than treating stress-fiber assembly as a core Cdc42 output. Proposed replacements: actin filament organization Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0051683 establishment of Golgi localization | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: establishment of Golgi localization is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0051835 positive regulation of synapse structural plasticity | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: positive regulation of synapse structural plasticity is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0051988 regulation of attachment of spindle microtubules to kinetochore | ISO GO_REF:0000119 | ACCEPT | Summary: regulation of attachment of spindle microtubules to kinetochore is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: UniProt:P60766 Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. |
| GO:0061630 ubiquitin protein ligase activity | ISO GO_REF:0000119 | REMOVE | Summary: Cdc42 is a small GTPase, not a ubiquitin-protein ligase. Reason: No direct evidence supports assigning E3 ligase catalytic activity to Cdc42; this appears to be an over-transfer from associated pathways or interactors. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0072384 organelle transport along microtubule | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: organelle transport along microtubule is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0072686 mitotic spindle | ISO GO_REF:0000119 | ACCEPT | Summary: mitotic spindle is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: UniProt:P60766 Cytoplasm, cytoskeleton, spindle |
| GO:0090135 actin filament branching | ISO GO_REF:0000096 | ACCEPT | Summary: actin filament branching is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0090316 positive regulation of intracellular protein transport | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: positive regulation of intracellular protein transport is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0098685 Schaffer collateral - CA1 synapse | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: Schaffer collateral - CA1 synapse is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0099159 regulation of modification of postsynaptic structure | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: regulation of modification of postsynaptic structure is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:1900026 positive regulation of substrate adhesion-dependent cell spreading | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: positive regulation of substrate adhesion-dependent cell spreading is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:24076653 CDC42 switches IRSp53 from inhibition of actin growth to elo... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:37140151 Biallelic variants in IQCN cause sperm flagellar assembly de... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0098794 postsynapse | IDA PMID:33531495 Photoactivatable CaMKII induces synaptic plasticity in singl... | KEEP AS NON CORE | Summary: postsynapse is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0098794 postsynapse | IMP PMID:33531495 Photoactivatable CaMKII induces synaptic plasticity in singl... | KEEP AS NON CORE | Summary: postsynapse is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0098978 glutamatergic synapse | IDA PMID:25851601 The RhoGEF DOCK10 is essential for dendritic spine morphogen... | KEEP AS NON CORE | Summary: glutamatergic synapse is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0098978 glutamatergic synapse | IMP PMID:25851601 The RhoGEF DOCK10 is essential for dendritic spine morphogen... | KEEP AS NON CORE | Summary: glutamatergic synapse is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0098978 glutamatergic synapse | IDA PMID:33531495 Photoactivatable CaMKII induces synaptic plasticity in singl... | KEEP AS NON CORE | Summary: glutamatergic synapse is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0098978 glutamatergic synapse | IMP PMID:33531495 Photoactivatable CaMKII induces synaptic plasticity in singl... | KEEP AS NON CORE | Summary: glutamatergic synapse is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0099175 regulation of postsynapse organization | IDA PMID:25851601 The RhoGEF DOCK10 is essential for dendritic spine morphogen... | KEEP AS NON CORE | Summary: regulation of postsynapse organization is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0099175 regulation of postsynapse organization | IMP PMID:25851601 The RhoGEF DOCK10 is essential for dendritic spine morphogen... | KEEP AS NON CORE | Summary: regulation of postsynapse organization is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0003015 heart process | IMP PMID:25197047 Essential role of sympathetic endothelin A receptors for adv... | KEEP AS NON CORE | Summary: heart process is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0003015 heart process | IMP PMID:31219639 Cdc42 activation by endothelin regulates neural crest cell m... | KEEP AS NON CORE | Summary: heart process is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0003253 cardiac neural crest cell migration involved in outflow tract morphogenesis | IMP PMID:31219639 Cdc42 activation by endothelin regulates neural crest cell m... | KEEP AS NON CORE | Summary: cardiac neural crest cell migration involved in outflow tract morphogenesis is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0006468 protein phosphorylation | IMP PMID:31219639 Cdc42 activation by endothelin regulates neural crest cell m... | MODIFY | Summary: Cdc42 does not itself catalyze protein phosphorylation. Reason: The evidence supports upstream small-GTPase signaling to kinase effectors such as PAK rather than direct kinase activity by Cdc42. Proposed replacements: small GTPase-mediated signal transduction Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0031252 cell leading edge | IDA PMID:31219639 Cdc42 activation by endothelin regulates neural crest cell m... | ACCEPT | Summary: cell leading edge is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0035050 embryonic heart tube development | IMP PMID:31219639 Cdc42 activation by endothelin regulates neural crest cell m... | KEEP AS NON CORE | Summary: embryonic heart tube development is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0086100 endothelin receptor signaling pathway | IMP PMID:31219639 Cdc42 activation by endothelin regulates neural crest cell m... | KEEP AS NON CORE | Summary: endothelin receptor signaling pathway is retained as a non-core Cdc42-associated annotation. Reason: The annotation is plausible as a downstream or compartment-specific consequence of Cdc42 signaling rather than the core GTPase switch activity. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:15710388 Zizimin2: a novel, DOCK180-related Cdc42 guanine nucleotide ... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:18604197 IQGAP3 regulates cell proliferation through the Ras/ERK sign... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:21828338 Cool-1/Ξ²PIX functions as a guanine nucleotide exchange facto... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:8464478 Oncogene ect2 is related to regulators of small GTP-binding ... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0003924 GTPase activity | ISO PMID:27917469 Association of dysfunctional synapse defective 1 (SYDE1) wit... | ACCEPT | Summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0048549 positive regulation of pinocytosis | IMP PMID:24792215 CLN3 deficient cells display defects in the ARF1-Cdc42 pathw... | ACCEPT | Summary: positive regulation of pinocytosis is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0045335 phagocytic vesicle | IDA PMID:19144319 The phagosomal proteome in interferon-gamma-activated macrop... | ACCEPT | Summary: phagocytic vesicle is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0071346 cellular response to type II interferon | IDA PMID:19144319 The phagosomal proteome in interferon-gamma-activated macrop... | KEEP AS NON CORE | Summary: cellular response to type II interferon is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0006911 phagocytosis, engulfment | ISS GO_REF:0000024 | ACCEPT | Summary: phagocytosis, engulfment is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0007015 actin filament organization | ISS GO_REF:0000024 | ACCEPT | Summary: actin filament organization is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0034329 cell junction assembly | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: cell junction assembly is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0045198 establishment of epithelial cell apical/basal polarity | ISS GO_REF:0000024 | ACCEPT | Summary: establishment of epithelial cell apical/basal polarity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0051491 positive regulation of filopodium assembly | IMP PMID:22494997 Age-related guanine nucleotide exchange factor, mouse Zizimi... | ACCEPT | Summary: positive regulation of filopodium assembly is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0060997 dendritic spine morphogenesis | IMP PMID:25851601 The RhoGEF DOCK10 is essential for dendritic spine morphogen... | KEEP AS NON CORE | Summary: dendritic spine morphogenesis is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0003924 GTPase activity | IDA PMID:18838382 FGD2, a CDC42-specific exchange factor expressed by antigen-... | ACCEPT | Summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0003924 GTPase activity | IGI PMID:18838382 FGD2, a CDC42-specific exchange factor expressed by antigen-... | ACCEPT | Summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0005515 protein binding | IPI PMID:18838382 FGD2, a CDC42-specific exchange factor expressed by antigen-... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0000322 storage vacuole | IDA PMID:21828338 Cool-1/Ξ²PIX functions as a guanine nucleotide exchange facto... | KEEP AS NON CORE | Summary: storage vacuole is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005829 cytosol | IDA PMID:21828338 Cool-1/Ξ²PIX functions as a guanine nucleotide exchange facto... | ACCEPT | Summary: cytosol is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0005886 plasma membrane | IDA PMID:21828338 Cool-1/Ξ²PIX functions as a guanine nucleotide exchange facto... | ACCEPT | Summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0005886 plasma membrane | IDA PMID:25217619 Phosphoinositide 3-kinase p85beta regulates invadopodium for... | ACCEPT | Summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0005886 plasma membrane | TAS Reactome:R-MMU-3928643 | ACCEPT | Summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0005886 plasma membrane | TAS Reactome:R-MMU-442572 | ACCEPT | Summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0005886 plasma membrane | TAS Reactome:R-MMU-448947 | ACCEPT | Summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0005886 plasma membrane | TAS Reactome:R-MMU-5665684 | ACCEPT | Summary: plasma membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0006897 endocytosis | IMP PMID:24792215 CLN3 deficient cells display defects in the ARF1-Cdc42 pathw... | KEEP AS NON CORE | Summary: PMID:24792215 directly links Cdc42 cycling to actin-dependent fluid-phase endocytosis. Reason: The process is supported for Cdc42-mediated actin cycling, but it remains a context-specific trafficking output rather than the core small-GTPase molecular function. Supporting Evidence: PMID:24792215 The small GTPase Cdc42 regulates sequential synthesis and break down of actin allowing fluid-phase uptake to occur |
| GO:0043209 myelin sheath | HDA PMID:17634366 Proteolipid protein is required for transport of sirtuin 2 i... | KEEP AS NON CORE | Summary: myelin sheath is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0031256 leading edge membrane | IDA PMID:22461490 DOCK8 is a Cdc42 activator critical for interstitial dendrit... | ACCEPT | Summary: leading edge membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0036336 dendritic cell migration | IGI PMID:22461490 DOCK8 is a Cdc42 activator critical for interstitial dendrit... | KEEP AS NON CORE | Summary: dendritic cell migration is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005525 GTP binding | ISO PMID:23620790 CBAP functions as a novel component in chemokine-induced ZAP... | ACCEPT | Summary: GTP binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0005911 cell-cell junction | IDA PMID:23793062 The lymphoid lineage-specific actin-uncapping protein Rltpr ... | KEEP AS NON CORE | Summary: cell-cell junction is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0016020 membrane | ISS GO_REF:0000024 | ACCEPT | Summary: membrane is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:1900026 positive regulation of substrate adhesion-dependent cell spreading | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: positive regulation of substrate adhesion-dependent cell spreading is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0060501 positive regulation of epithelial cell proliferation involved in lung morphogenesis | IMP PMID:23219958 CDC42 is required for structural patterning of the lung duri... | KEEP AS NON CORE | Summary: positive regulation of epithelial cell proliferation involved in lung morphogenesis is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:10954424 The mammalian homologue of the Caenorhabditis elegans polari... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:11035016 A new family of Cdc42 effector proteins, CEPs, function in f... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0003161 cardiac conduction system development | IGI PMID:21690310 Tinman/Nkx2-5 acts via miR-1 and upstream of Cdc42 to regula... | KEEP AS NON CORE | Summary: cardiac conduction system development is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0060047 heart contraction | IGI PMID:21690310 Tinman/Nkx2-5 acts via miR-1 and upstream of Cdc42 to regula... | KEEP AS NON CORE | Summary: heart contraction is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0019901 protein kinase binding | IPI PMID:21048939 ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange facto... | ACCEPT | Summary: protein kinase binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0005515 protein binding | IPI PMID:17000758 Ccpg1, a novel scaffold protein that regulates the activity ... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0030496 midbody | ISS GO_REF:0000024 | ACCEPT | Summary: midbody is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0032467 positive regulation of cytokinesis | ISS GO_REF:0000024 | ACCEPT | Summary: positive regulation of cytokinesis is supported for Cdc42, but this is a cell-cycle output rather than the small GTPase catalytic role itself. Reason: UniProt carries this transferred process annotation, but it is treated as a downstream cell-cycle output rather than core GTPase catalysis. Supporting Evidence: UniProt:P60766 GO; GO:0032467; P:positive regulation of cytokinesis; ISS:UniProtKB. |
| GO:0051233 spindle midzone | ISS GO_REF:0000024 | ACCEPT | Summary: spindle midzone is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: UniProt:P60766 Moves to the central spindle as cells progressed through anaphase to telophase. |
| GO:0051489 regulation of filopodium assembly | ISS GO_REF:0000024 | ACCEPT | Summary: regulation of filopodium assembly is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0051988 regulation of attachment of spindle microtubules to kinetochore | ISS GO_REF:0000024 | ACCEPT | Summary: regulation of attachment of spindle microtubules to kinetochore is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: UniProt:P60766 Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. |
| GO:0072686 mitotic spindle | ISS GO_REF:0000024 | ACCEPT | Summary: mitotic spindle is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: UniProt:P60766 Cytoplasm, cytoskeleton, spindle |
| GO:0005737 cytoplasm | IDA PMID:19796622 Uncovering early response of gene regulatory networks in ESC... | ACCEPT | Summary: cytoplasm is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0071944 cell periphery | IDA PMID:19796622 Uncovering early response of gene regulatory networks in ESC... | ACCEPT | Summary: cell periphery is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0005737 cytoplasm | IDA PMID:15775979 E-cadherin is essential for in vivo epidermal barrier functi... | ACCEPT | Summary: cytoplasm is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 associates with the **plasma membrane/cell cortex** via a **carboxy-terminal prenyl group** |
| GO:0043410 positive regulation of MAPK cascade | IDA PMID:19244314 Cdo promotes neuronal differentiation via activation of the ... | KEEP AS NON CORE | Summary: positive regulation of MAPK cascade is supported in a Cdo/JLP/Bnip-2 neuronal differentiation context, but it is not Cdc42's core molecular role. Reason: The cited evidence supports a context-specific Cdc42/p38 MAPK signaling output. Supporting Evidence: PMID:19244314 These molecules regulate Cdc42 and p38MAPK activities, which increase in a Cdo-dependent manner during neuronal differentiation of C17.2 cells and retinoic acid-treated P19 cells. |
| GO:0003924 GTPase activity | ISA PMID:12637522 Multifunctional roles for the PH domain of Dbs in regulating... | ACCEPT | Summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0007097 nuclear migration | IMP PMID:16892058 The Rho-GTPase cdc42 regulates neural progenitor fate at the... | KEEP AS NON CORE | Summary: nuclear migration is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0008104 intracellular protein localization | IMP PMID:16621792 Membrane localization of adenomatous polyposis coli protein ... | KEEP AS NON CORE | Summary: intracellular protein localization is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0034332 adherens junction organization | IMP PMID:16892058 The Rho-GTPase cdc42 regulates neural progenitor fate at the... | KEEP AS NON CORE | Summary: adherens junction organization is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0035088 establishment or maintenance of apical/basal cell polarity | IMP PMID:16892058 The Rho-GTPase cdc42 regulates neural progenitor fate at the... | ACCEPT | Summary: establishment or maintenance of apical/basal cell polarity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0051647 nucleus localization | IMP PMID:16892058 The Rho-GTPase cdc42 regulates neural progenitor fate at the... | KEEP AS NON CORE | Summary: nucleus localization is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0007088 regulation of mitotic nuclear division | IMP PMID:16892058 The Rho-GTPase cdc42 regulates neural progenitor fate at the... | KEEP AS NON CORE | Summary: regulation of mitotic nuclear division is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0045177 apical part of cell | IDA PMID:16892058 The Rho-GTPase cdc42 regulates neural progenitor fate at the... | KEEP AS NON CORE | Summary: apical part of cell is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0048664 neuron fate determination | IMP PMID:16892058 The Rho-GTPase cdc42 regulates neural progenitor fate at the... | KEEP AS NON CORE | Summary: neuron fate determination is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0007163 establishment or maintenance of cell polarity | TAS PMID:10954424 The mammalian homologue of the Caenorhabditis elegans polari... | ACCEPT | Summary: establishment or maintenance of cell polarity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0016197 endosomal transport | TAS PMID:10954424 The mammalian homologue of the Caenorhabditis elegans polari... | KEEP AS NON CORE | Summary: endosomal transport is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0098609 cell-cell adhesion | TAS PMID:10954424 The mammalian homologue of the Caenorhabditis elegans polari... | KEEP AS NON CORE | Summary: cell-cell adhesion is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0007097 nuclear migration | IMP PMID:15882626 Nuclear movement regulated by Cdc42, MRCK, myosin, and actin... | KEEP AS NON CORE | Summary: nuclear migration is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:15996550 The Rho-specific GEF Lfc interacts with neurabin and spinoph... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0042995 cell projection | IDA PMID:15728722 Local phosphatidylinositol 3,4,5-trisphosphate accumulation ... | KEEP AS NON CORE | Summary: cell projection is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0051897 positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction | IMP PMID:15728722 Local phosphatidylinositol 3,4,5-trisphosphate accumulation ... | KEEP AS NON CORE | Summary: positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction is a supported context-specific Cdc42 output. Reason: Cdc42 controls broad cellular architecture and developmental programs through actin, polarity, trafficking, and kinase-effector pathways, but this term is not the core molecular function. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:16052498 Expression, synaptic localization, and developmental regulat... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0005515 protein binding | IPI PMID:12773384 TCGAP, a multidomain Rho GTPase-activating protein involved ... | MARK AS OVER ANNOTATED | Summary: protein binding records a physical association but is too generic to describe the gene product function. Reason: The evidence supports regulated signaling-complex assembly, not a meaningful standalone protein binding function; more informative molecular and pathway terms capture the biology. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md In its **GTP-bound state**, CDC42 binds effector proteins that drive downstream cytoskeletal and polarity responses |
| GO:0003924 GTPase activity | IDA PMID:15249579 Vav GEFs are required for beta2 integrin-dependent functions... | ACCEPT | Summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0030742 GTP-dependent protein binding | IPI PMID:10954424 The mammalian homologue of the Caenorhabditis elegans polari... | ACCEPT | Summary: GTP-dependent protein binding is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0003924 GTPase activity | IDA PMID:10699171 Functional analysis of ARHGAP6, a novel GTPase-activating pr... | ACCEPT | Summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0046847 filopodium assembly | IDA PMID:10699171 Functional analysis of ARHGAP6, a novel GTPase-activating pr... | ACCEPT | Summary: filopodium assembly is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
| GO:0003924 GTPase activity | TAS PMID:10490598 The Borgs, a new family of Cdc42 and TC10 GTPase-interacting... | ACCEPT | Summary: GTPase activity is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is a **GTP-hydrolyzing enzyme** (small GTPase). Its substrate is **GTP**, which it binds and hydrolyzes to **GDP + Pi** |
| GO:0007266 Rho protein signal transduction | TAS PMID:10490598 The Borgs, a new family of Cdc42 and TC10 GTPase-interacting... | ACCEPT | Summary: Rho protein signal transduction is consistent with core Cdc42 GTPase, cytoskeletal, polarity, or localization biology. Reason: The term directly reflects Cdc42 GDP/GTP switch activity, effector binding, membrane localization, or immediate actin/polarity outputs. Supporting Evidence: file:mouse/Cdc42/Cdc42-deep-research-falcon.md CDC42 is directly linked to actin nucleation pathways through **WASP-family nucleation-promoting factors**. |
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Download this section (compressed HTML)Q: Which mouse Cdc42 annotations should be isoform-qualified for prenylated versus palmitoylated Cdc42 products?
Q: Which neuronal/synaptic Cdc42 terms are directly supported for the brain-enriched isoform rather than inferred from general Rho-family biology?
Experiment: Use isoform-specific knock-in tags and rescue alleles to measure Cdc42 effector recruitment at leading edge, Golgi/endosomal, midbody, and neuronal compartments.
Hypothesis: Cdc42 pleiotropy is primarily explained by isoform- and lipidation-dependent localization rather than different intrinsic GTPase biochemistry.
Type: Live-cell imaging and isoform-specific rescue
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