Mir100

Summary: Rfam2GO annotation placing the mature miRNA in RISC.

Reason: Mature miR-100 is loaded into Argonaute/RISC; this is core to how the gene product works.

Summary: Rfam2GO annotation of the generic microRNA process.

Reason: Correct and core for a mir-100 precursor.

Summary: Direct-assay support for miR-100-mediated repression of Smarca5.

Reason: Smarca5 was shown to be a direct target of miR-100, which is post-transcriptional silencing by the mature miRNA.

Summary: The functionally validated role from this paper, established by anti-miR suppression and overexpression.

Reason: miR-100 is one of the three miRNAs the study tested rather than merely profiled: suppression by anti-miRs blocked ESC differentiation and overexpression altered differentiation markers. This is the correct representation of what the paper shows about mir-100, and it is the reason the LIF row below is redundant as well as unsupported.

Summary: Batch expression row from the same paper whose validated finding is already captured by the IMP annotation above; the term also misdescribes the experiment.

Reason: This row and the IMP row above come from the same paper, and the contrast is the point. The IMP row records what was demonstrated - miR-100 is required for ESC differentiation via Smarca5. This IEP row is one of 291 annotations to a single term generated from the same study's expression survey. The term is also a poor description of the experiment: LIF was withdrawn to induce differentiation, so the miRNAs went up in the absence of LIF, which is not a cellular response to LIF. Even for a miRNA the paper did validate, the batch row adds a wrong term rather than support.

Proposed replacements: positive regulation of stem cell differentiation

Summary: Derived from an ageing expression survey that never names mir-100, and pointed at normal hearing when the study is about its loss.

Reason: Two independent problems. First, the PMC full text of the cited paper contains no mention of miR-100, so the annotation comes from a supplementary microarray table listing miRNAs whose abundance shifted with age; nothing connects mir-100 to auditory function. Second, the study is about degeneration of the organ of Corti during age-related hearing loss, and its own conclusion is explicitly correlative - expression changes precede functional change. Annotating mir-100 as acting upstream of or within the normal process of sound perception inverts what was measured. One of 100 IEP rows to this term from this paper.

Summary: mir-100 is not discussed anywhere in the cited paper; the row reflects membership of the detected-miRNA set.

Reason: The bottom tier of the 130-member IEP batch from this study. The paper detected 372 miRNAs, found 12 that changed, and functionally validated three; miR-100 is in none of those groups and the PMC full text does not mention it. The only fact behind this annotation is that mir-100 was present at detectable levels in hippocampal slices, which is true of hundreds of miRNAs and says nothing about LTP.

Summary: Localization of pri-miRNA transcripts to post-synaptic densities.

Reason: The cited study directly assays pri-miRNA localization at post-synaptic densities in adult mouse forebrain, which supports a synaptic location. Kept as non-core because localization is not the activity that defines mir-100.

Summary: Cannot be adjudicated; the cached full text does not mention mir-100.

Reason: PMC full text is cached for this reference and contains no mention of miR-100; the paper concerns miR-7b inhibiting Fos translation after hyperosmolar stimulation. Because the cached text may be incomplete and the curator saw the published figures, this is left undecided rather than removed.

Summary: Retained on the curator's authority; the cited paper does discuss miR-100.

Reason: Unlike several other references on this gene, the cached full text of this Dicer-ablation study does mention miR-100, so the curator had direct material. Kept as non-core because a Schwann-cell myelination phenotype from global Dicer loss is a pathway-level context rather than a demonstrated mir-100 activity.