Mir30e encodes the mouse mir-30e precursor, a member of the miR-30 family. Its mature product is a guide RNA directing Argonaute/RISC to complementary sites in target mRNAs, which is the only well-established function of this gene. No specific downstream process has been established for mir-30e by functional perturbation; its current GOA process annotations all derive from surveys in which mir-30e was one of many miRNAs whose abundance changed.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0016442 RISC complex | IEA GO_REF:0000115 | ACCEPT | Summary: Rfam2GO annotation placing the mature miRNA in RISC. Reason: Mature miR-30e is loaded into Argonaute/RISC; this is core to how the gene product works. |
| GO:0035195 miRNA-mediated post-transcriptional gene silencing | IEA GO_REF:0000115 | ACCEPT | Summary: Rfam2GO annotation of the generic microRNA process. Reason: Correct and, in the absence of any functionally established downstream process, the only core biological process for this gene. |
| GO:0009617 response to bacterium | IEP PMID:24205035 Profiling circulating microRNA expression in experimental se... | MARK AS OVER ANNOTATED | Summary: Bulk microarray table entry from a circulating-biomarker survey whose own controls argue against a bacterium-sensing mechanism. Reason: mir-30e is not one of the 10 miRNAs this paper carried forward to quantitative PCR validation; it appears only in the table of 45 miRNAs raised at 24 h. Even for the validated 10 the authors showed the increase persists in Tlr2, Tlr4 and NF-kB knockouts and concluded bacterial sensing was not the driver. So the observation behind this row is a change in the blood level of one miRNA among dozens, in a surgical sepsis model, with the bacterial mechanism explicitly excluded. One of 58 IEP rows to this term from this single paper. Supporting Evidence: PMID:24205035 At 24 h after injection, the levels of 45 miRNAs increased in the whole blood PMID:24205035 which indicated that the transcription of these miRNAs was not directly mediated by the TLR2/NF-ΞΊB or TLR4/NF-ΞΊB pathway, and pathways induced by exposure to the gram-positive or gram-negative bacteria |
| GO:0071361 cellular response to ethanol | IEP PMID:19091803 Ethanol exposure induces differential microRNA and target ge... | MARK AS OVER ANNOTATED | Summary: Expression change under ethanol exposure, with no evidence that mir-30e participates in the response. Reason: The cited study is a differential expression survey of ethanol-exposed embryos. A transcript whose level moves under ethanol is not thereby a component of the cellular ethanol response; GO's own guidance reserves "response to" terms for products required for the response to occur. The cached record is abstract-only, so this is marked as over-annotated rather than removed - the expression observation itself is not in doubt, only the inference from it. |
| GO:0060291 long-term synaptic potentiation | IEP PMID:25858512 miR-26a and miR-384-5p are required for LTP maintenance and ... | MARK AS OVER ANNOTATED | Summary: Genuinely differentially expressed during LTP, but never functionally tested; the paper itself calls such miRNAs candidates. Reason: This is the informative middle tier of the 130-member IEP batch from this paper. Unlike most of the cohort, mir-30e really did change - it is named as one of the six downregulated miRNAs among the 12 that changed during LTP - so the IEP evidence code is used correctly and the observation is sound. What is not established is agency: the authors tested only miR-26a, miR-384-5p and let-7a, and describe the rest as a catalogue of candidate LTP miRNAs. "Acts upstream of or within long-term synaptic potentiation" promotes a candidate to a participant. Marked over-annotated rather than removed precisely because the underlying expression change is real. Supporting Evidence: PMID:25858512 two of the downregulated miRNAs (miR-384-5p and miR-30e) belong to the same miRNA family PMID:25858512 presents a catalogue of candidate 'LTP miRNAs' |
| GO:0070482 response to oxygen levels | IDA PMID:19188439 MicroRNA-1 negatively regulates expression of the hypertroph... | UNDECIDED | Summary: Cannot be adjudicated from the cached record. Reason: The cached publication is abstract-only and concerns miR-1 regulating calmodulin and Mef2a, with no mention of miR-30 in the abstract. The curator will have read the full text, so this is left undecided pending full-text access rather than challenged. |
| GO:0071230 cellular response to amino acid stimulus | IDA PMID:20548288 Difference in expression of hepatic microRNAs miR-29c, miR-3... | UNDECIDED | Summary: Cannot be adjudicated from the cached record. Reason: The cached publication is abstract-only and its title concerns hepatic miR-29c, miR-34a, miR-155 and miR-200b in dietary NAFLD susceptibility, with no mention of miR-30. Left undecided pending full text. |
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Download this section (compressed HTML)Q: Does mir-30e have any demonstrated function in long-term potentiation beyond being downregulated during it, given that its family member miR-384-5p is required?
Suggested experts: synaptic plasticity researchers, microRNA biologists
Q: Do the miR-30 family members act redundantly, such that single-miRNA perturbation would miss a real contribution?
Suggested experts: microRNA biologists
Experiment: Antagomir or sponge inhibition of miR-30e in hippocampal slices, with LTP recording and spine imaging, matching the assays used for miR-26a and miR-384-5p
Hypothesis: miR-30e is dispensable for LTP maintenance despite being downregulated during LTP, distinguishing correlation from requirement
Type: electrophysiology
Experiment: Ago CLIP in hippocampal neurons to identify miR-30e-dependent target occupancy
Hypothesis: miR-30e has a distinct target set from miR-384-5p despite shared family membership
Type: high-throughput sequencing
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