Rbpj (RBP-Jkappa; CSL/CBF1, the mammalian Suppressor of Hairless) is a ubiquitously expressed nuclear, sequence-specific DNA-binding transcription factor that recognises the CGTGGGAA motif and is the central DNA-binding effector of canonical Notch signalling. In the absence of Notch signal it represses target genes by recruiting corepressor complexes (e.g. SHARP/MINT with CtIP/CtBP, KyoT2, L3MBTL3 with KDM1A/LSD1). On Notch activation, the Notch intracellular domain binds Rbpj through its RAM and ankyrin repeats, inducing an allosteric change that recruits Mastermind-like coactivators and converts Rbpj into an activator within the CSL-NICD-MAML ternary complex, driving transcription of targets such as Hes and Hey genes. Independently of Notch, Rbpj also partners with the bHLH factor Ptf1a in the PTF1-J complex in early pancreas and neural tube. Loss of Rbpj abolishes canonical Notch signalling and causes pleiotropic defects in somitogenesis, cardiovascular development, neurogenesis, lymphocyte and myeloid differentiation and stem-cell maintenance.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000122 negative regulation of transcription by RNA polymerase II | IEA GO_REF:0000120 | ACCEPT | Summary: Rbpj represses Notch target genes in the absence of NICD by recruiting corepressor complexes (SHARP/CtIP/CtBP, KyoT2, L3MBTL3-KDM1A). Reason: Repression is one half of the core CSL switch function. Supporting Evidence: PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. |
| GO:0000122 negative regulation of transcription by RNA polymerase II | IMP PMID:14701881 Notch promotes epithelial-mesenchymal transition during card... | ACCEPT | Summary: Rbpj represses Notch target genes in the absence of NICD by recruiting corepressor complexes (SHARP/CtIP/CtBP, KyoT2, L3MBTL3-KDM1A). Reason: Repression is one half of the core CSL switch function. Supporting Evidence: PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. |
| GO:0000122 negative regulation of transcription by RNA polymerase II | IMP PMID:17336907 Notch signaling is essential for ventricular chamber develop... | ACCEPT | Summary: Rbpj represses Notch target genes in the absence of NICD by recruiting corepressor complexes (SHARP/CtIP/CtBP, KyoT2, L3MBTL3-KDM1A). Reason: Repression is one half of the core CSL switch function. Supporting Evidence: PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. |
| GO:0000122 negative regulation of transcription by RNA polymerase II | IMP PMID:21311046 Differential Notch signaling in the epicardium is required f... | ACCEPT | Summary: Rbpj represses Notch target genes in the absence of NICD by recruiting corepressor complexes (SHARP/CtIP/CtBP, KyoT2, L3MBTL3-KDM1A). Reason: Repression is one half of the core CSL switch function. Supporting Evidence: PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. |
| GO:0000122 negative regulation of transcription by RNA polymerase II | ISO GO_REF:0000119 | ACCEPT | Summary: Rbpj represses Notch target genes in the absence of NICD by recruiting corepressor complexes (SHARP/CtIP/CtBP, KyoT2, L3MBTL3-KDM1A). Reason: Repression is one half of the core CSL switch function. Supporting Evidence: PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. |
| GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding | IBA GO_REF:0000033 | ACCEPT | Summary: Rbpj is a sequence-specific DNA-binding protein recognising the CGTGGGAA (CSL) motif in Pol II promoters/enhancers of Notch targets and PTF1 targets. Reason: Sequence-specific DNA binding is the defining molecular property of CSL proteins. Supporting Evidence: PMID:8749394 The mammalian transcription factor RBP-J kappa binds to the DNA sequence motif CGTGGGAA and is involved in the regulation of gene expression |
| GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding | IDA PMID:17658279 Direct regulation of Gata3 expression determines the T helpe... | ACCEPT | Summary: Rbpj is a sequence-specific DNA-binding protein recognising the CGTGGGAA (CSL) motif in Pol II promoters/enhancers of Notch targets and PTF1 targets. Reason: Sequence-specific DNA binding is the defining molecular property of CSL proteins. Supporting Evidence: PMID:8749394 The mammalian transcription factor RBP-J kappa binds to the DNA sequence motif CGTGGGAA and is involved in the regulation of gene expression |
| GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding | IEA GO_REF:0000120 | ACCEPT | Summary: Rbpj is a sequence-specific DNA-binding protein recognising the CGTGGGAA (CSL) motif in Pol II promoters/enhancers of Notch targets and PTF1 targets. Reason: Sequence-specific DNA binding is the defining molecular property of CSL proteins. Supporting Evidence: PMID:8749394 The mammalian transcription factor RBP-J kappa binds to the DNA sequence motif CGTGGGAA and is involved in the regulation of gene expression |
| GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding | ISO GO_REF:0000119 | ACCEPT | Summary: Rbpj is a sequence-specific DNA-binding protein recognising the CGTGGGAA (CSL) motif in Pol II promoters/enhancers of Notch targets and PTF1 targets. Reason: Sequence-specific DNA binding is the defining molecular property of CSL proteins. Supporting Evidence: PMID:8749394 The mammalian transcription factor RBP-J kappa binds to the DNA sequence motif CGTGGGAA and is involved in the regulation of gene expression |
| GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific | IBA GO_REF:0000033 | ACCEPT | Summary: IBA (PANTHER:PTN000071433, CSL family node) Pol II-specific DNA-binding TF activity. Reason: Correct and appropriately general for a bifunctional (repressor/activator) DNA-binding transcription factor; core MF. Supporting Evidence: PMID:24290140 CSL dually functions as an activator and a repressor of transcription through differential interactions with coactivator or corepressor proteins, respectively. file:mouse/Rbpj/Rbpj-deep-research-falcon.md In the absence of Notch signaling, RBPJ remains constitutively bound to target-gene regulatory elements and actively represses transcription by recruiting corepressor complexes |
| GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific | IDA PMID:12730124 Notch signaling regulates left-right asymmetry determination... | ACCEPT | Summary: DNA-binding transcription activator activity. Rbpj activates transcription when bound by NICD and Mastermind (e.g. Nodal node enhancer, Slug, Notch targets). Reason: Rbpj acts as a DNA-binding activator in the CSL-NICD-MAML complex; accurate though context-dependent (it is also a repressor). Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific | IDA PMID:17336907 Notch signaling is essential for ventricular chamber develop... | ACCEPT | Summary: DNA-binding transcription activator activity. Rbpj activates transcription when bound by NICD and Mastermind (e.g. Nodal node enhancer, Slug, Notch targets). Reason: Rbpj acts as a DNA-binding activator in the CSL-NICD-MAML complex; accurate though context-dependent (it is also a repressor). Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific | IEA GO_REF:0000120 | ACCEPT | Summary: DNA-binding transcription activator activity. Rbpj activates transcription when bound by NICD and Mastermind (e.g. Nodal node enhancer, Slug, Notch targets). Reason: Rbpj acts as a DNA-binding activator in the CSL-NICD-MAML complex; accurate though context-dependent (it is also a repressor). Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific | ISO PMID:18663143 Slug is a direct Notch target required for initiation of car... | ACCEPT | Summary: DNA-binding transcription activator activity. Rbpj activates transcription when bound by NICD and Mastermind (e.g. Nodal node enhancer, Slug, Notch targets). Reason: Rbpj acts as a DNA-binding activator in the CSL-NICD-MAML complex; accurate though context-dependent (it is also a repressor). Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0001525 angiogenesis | IMP PMID:15466160 Haploinsufficient lethality and formation of arteriovenous m... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0001756 somitogenesis | IMP PMID:19779553 Notch is a critical component of the mouse somitogenesis osc... | KEEP AS NON CORE | Summary: Rbpj-/- embryos (lacking all canonical Notch activity) fail to form proper somites and lose clock gene oscillation. Reason: Genuine requirement via canonical Notch signalling in the segmentation clock, but a tissue-specific consequence of its core CSL function. Supporting Evidence: PMID:19779553 To more definitively test the relevance of Notch activity during the process of somitogenesis we decided to re-analyse homozygous null embryos from two other mutant lines widely accepted to develop in the complete absence of Notch activity, Rbpjβ/β and Psen1β/β;Psen2β/β |
| GO:0001756 somitogenesis | TAS PMID:15466160 Haploinsufficient lethality and formation of arteriovenous m... | KEEP AS NON CORE | Summary: Rbpj-/- embryos (lacking all canonical Notch activity) fail to form proper somites and lose clock gene oscillation. Reason: Genuine requirement via canonical Notch signalling in the segmentation clock, but a tissue-specific consequence of its core CSL function. Supporting Evidence: PMID:19779553 To more definitively test the relevance of Notch activity during the process of somitogenesis we decided to re-analyse homozygous null embryos from two other mutant lines widely accepted to develop in the complete absence of Notch activity, Rbpjβ/β and Psen1β/β;Psen2β/β |
| GO:0001837 epithelial to mesenchymal transition | IMP PMID:14701881 Notch promotes epithelial-mesenchymal transition during card... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0001947 heart looping | TAS PMID:15466160 Haploinsufficient lethality and formation of arteriovenous m... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0001974 blood vessel remodeling | IMP PMID:15466160 Haploinsufficient lethality and formation of arteriovenous m... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0002193 MAML1-RBP-Jkappa- ICN1 complex | IBA GO_REF:0000033 | ACCEPT | Summary: CSL-NICD-Mastermind ternary transcription complex. Reason: Rbpj is the DNA-binding core of this complex. Supporting Evidence: PMID:18381292 we show that the addition of a RAM peptide in trans facilitates formation of the CSL-NotchIC-Mastermind ternary complex in vitro. |
| GO:0002193 MAML1-RBP-Jkappa- ICN1 complex | IEA GO_REF:0000107 | ACCEPT | Summary: CSL-NICD-Mastermind ternary transcription complex. Reason: Rbpj is the DNA-binding core of this complex. Supporting Evidence: PMID:18381292 we show that the addition of a RAM peptide in trans facilitates formation of the CSL-NotchIC-Mastermind ternary complex in vitro. |
| GO:0002193 MAML1-RBP-Jkappa- ICN1 complex | ISO GO_REF:0000119 | ACCEPT | Summary: CSL-NICD-Mastermind ternary transcription complex. Reason: Rbpj is the DNA-binding core of this complex. Supporting Evidence: PMID:18381292 we show that the addition of a RAM peptide in trans facilitates formation of the CSL-NotchIC-Mastermind ternary complex in vitro. |
| GO:0002437 inflammatory response to antigenic stimulus | IMP PMID:17658279 Direct regulation of Gata3 expression determines the T helpe... | KEEP AS NON CORE | Summary: Immune/haematopoietic phenotypes of Rbpj conditional loss (marginal zone B cells, Th2/Gata3, dendritic cells, Gata2-dependent haematopoiesis). Reason: Consequences of loss of Notch-RBP-J transcriptional control in specific immune lineages; pleiotropic, non-core. |
| GO:0003129 heart induction | TAS PMID:15466160 Haploinsufficient lethality and formation of arteriovenous m... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0003139 secondary heart field specification | IGI PMID:22711842 Wnt/Ξ²-catenin and Bmp signals control distinct sets of trans... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0003139 secondary heart field specification | IMP PMID:22711842 Wnt/Ξ²-catenin and Bmp signals control distinct sets of trans... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0003151 outflow tract morphogenesis | IMP PMID:14701881 Notch promotes epithelial-mesenchymal transition during card... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0003157 endocardium development | IMP PMID:17336907 Notch signaling is essential for ventricular chamber develop... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0003160 endocardium morphogenesis | IMP PMID:14701881 Notch promotes epithelial-mesenchymal transition during card... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0003176 aortic valve development | IMP PMID:26491108 Notch-Tnf signalling is required for development and homeost... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0003177 pulmonary valve development | IMP PMID:26491108 Notch-Tnf signalling is required for development and homeost... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0003198 epithelial to mesenchymal transition involved in endocardial cushion formation | IMP PMID:14701881 Notch promotes epithelial-mesenchymal transition during card... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0003214 cardiac left ventricle morphogenesis | IMP PMID:21493891 Diet-induced aortic valve disease in mice haploinsufficient ... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0003222 ventricular trabecula myocardium morphogenesis | IMP PMID:17336907 Notch signaling is essential for ventricular chamber develop... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0003677 DNA binding | IDA PMID:9111338 RBP-L, a transcription factor related to RBP-Jkappa. | ACCEPT | Summary: Rbpj is a sequence-specific DNA-binding protein recognising the CGTGGGAA (CSL) motif in Pol II promoters/enhancers of Notch targets and PTF1 targets. Reason: Sequence-specific DNA binding is the defining molecular property of CSL proteins. Supporting Evidence: PMID:8749394 The mammalian transcription factor RBP-J kappa binds to the DNA sequence motif CGTGGGAA and is involved in the regulation of gene expression |
| GO:0003677 DNA binding | IEA GO_REF:0000002 | ACCEPT | Summary: Rbpj is a sequence-specific DNA-binding protein recognising the CGTGGGAA (CSL) motif in Pol II promoters/enhancers of Notch targets and PTF1 targets. Reason: Sequence-specific DNA binding is the defining molecular property of CSL proteins. Supporting Evidence: PMID:8749394 The mammalian transcription factor RBP-J kappa binds to the DNA sequence motif CGTGGGAA and is involved in the regulation of gene expression |
| GO:0003677 DNA binding | ISO PMID:11741889 HERP1 is a cell type-specific primary target of Notch. | ACCEPT | Summary: Rbpj is a sequence-specific DNA-binding protein recognising the CGTGGGAA (CSL) motif in Pol II promoters/enhancers of Notch targets and PTF1 targets. Reason: Sequence-specific DNA binding is the defining molecular property of CSL proteins. Supporting Evidence: PMID:8749394 The mammalian transcription factor RBP-J kappa binds to the DNA sequence motif CGTGGGAA and is involved in the regulation of gene expression |
| GO:0003682 chromatin binding | IDA PMID:17015435 Sustained Notch signaling in progenitors is required for seq... | ACCEPT | Summary: ChIP-based chromatin binding at Notch target loci. Reason: Consistent with Rbpj occupancy of target enhancers; non-specific but correct. Supporting Evidence: PMID:17336907 Our ChIP assays demonstrated that N1ICD/RBPJK binds to two conserved RBPJK responsive elements within the EphrinB2 genomic DNA PMID:23639443 Ptf1a is a component of a trimeric transcription complex (PTF1) that also includes an E-protein and Rbpj and binds a DNA motif containing an E-box and a TC-box |
| GO:0003682 chromatin binding | IDA PMID:23571214 BEND6 is a nuclear antagonist of Notch signaling during self... | ACCEPT | Summary: MGI row citing the BEND6 paper, whose chromatin immunoprecipitations all pull down BEND6 (anti-mBEND6, or anti-V5 on mBEND6-V5), not Rbpj/CBF1. Reason: Chromatin binding is true of Rbpj, which occupies Notch target enhancers (supported here by ChIP of RBPJK at EphrinB2 and by the PTF1 complex). The cited paper shows only that CBF1 is needed to recruit BEND6 to the Hes5 promoter; the chromatin-binding assays it reports are for BEND6, so the row is accepted on other evidence and flagged upstream. Supporting Evidence: PMID:17336907 Our ChIP assays demonstrated that N1ICD/RBPJK binds to two conserved RBPJK responsive elements within the EphrinB2 genomic DNA PMID:23639443 Ptf1a is a component of a trimeric transcription complex (PTF1) that also includes an E-protein and Rbpj and binds a DNA motif containing an E-box and a TC-box |
| GO:0003682 chromatin binding | IDA PMID:23639443 Prdm13 mediates the balance of inhibitory and excitatory neu... | ACCEPT | Summary: ChIP-based chromatin binding at Notch target loci. Reason: Consistent with Rbpj occupancy of target enhancers; non-specific but correct. Supporting Evidence: PMID:17336907 Our ChIP assays demonstrated that N1ICD/RBPJK binds to two conserved RBPJK responsive elements within the EphrinB2 genomic DNA PMID:23639443 Ptf1a is a component of a trimeric transcription complex (PTF1) that also includes an E-protein and Rbpj and binds a DNA motif containing an E-box and a TC-box |
| GO:0003700 DNA-binding transcription factor activity | IEA GO_REF:0000002 | ACCEPT | Summary: InterPro-derived DNA-binding transcription factor activity. Reason: Correct, general parent of the Pol II-specific term. |
| GO:0005515 protein binding | IPI PMID:11318877 p48 subunit of mouse PTF1 binds to RBP-Jkappa/CBF-1, the int... | MODIFY | Summary: Rbpj binds Ptf1a/p48 (Q9QX98), a bHLH transcription factor. Reason: Generic protein binding; informative term is DNA-binding transcription factor binding. Proposed replacements: DNA-binding transcription factor binding Supporting Evidence: PMID:11318877 p48 bound to RBP-Jkappa more strongly than and in a distinct way from Notch1. |
| GO:0005515 protein binding | IPI PMID:17210915 Baf60c is a nuclear Notch signaling component required for t... | MODIFY | Summary: IPI rows with Notch1 (Q01705) and Baf60c/Smarcd3 (Q6P9Z1); Baf60c stabilises NICD-RBP-J interaction. Reason: Generic protein binding; the Notch1 interaction is informatively captured by Notch binding. The Baf60c interaction supports no specific MF term for Rbpj. Proposed replacements: Notch binding Supporting Evidence: PMID:17210915 functions to stabilize interactions between activated Notch and its DNA-binding partner, RBP-J. |
| GO:0005515 protein binding | IPI PMID:17210915 Baf60c is a nuclear Notch signaling component required for t... | MODIFY | Summary: IPI rows with Notch1 (Q01705) and Baf60c/Smarcd3 (Q6P9Z1); Baf60c stabilises NICD-RBP-J interaction. Reason: Generic protein binding; the Notch1 interaction is informatively captured by Notch binding. The Baf60c interaction supports no specific MF term for Rbpj. Proposed replacements: Notch binding Supporting Evidence: PMID:17210915 functions to stabilize interactions between activated Notch and its DNA-binding partner, RBP-J. |
| GO:0005515 protein binding | IPI PMID:17283045 Rbm15 modulates Notch-induced transcriptional activation and... | REMOVE | Summary: IPI with Rbm15 (Q0VBL3), which modulates Notch-induced transcriptional activation. Reason: Generic protein binding is uninformative and the interaction does not define a specific molecular function of Rbpj; removal does not dispute the interaction. |
| GO:0005515 protein binding | IPI PMID:17392792 Opposing LSD1 complexes function in developmental gene activ... | MODIFY | Summary: Rbpj binds corepressors: KyoT2 (Fhl1 isoform), L3MBTL3, and LSD1/KDM1A-containing corepressor complexes. Reason: Generic protein binding; these are corepressor interactions mediating Rbpj repression, best captured as transcription corepressor binding. Proposed replacements: transcription corepressor binding Supporting Evidence: PMID:24290140 our studies provide molecular insights into how KyoT2 binds CSL with high affinity and competes with coactivators, such as Notch, for binding CSL. PMID:29030483 L3MBTL3 competes with NOTCH ICD for binding to RBPJ |
| GO:0005515 protein binding | IPI PMID:22258620 Pten coordinates retinal neurogenesis by regulating Notch si... | MODIFY | Summary: Rbpj binds the intracellular domains of Notch1-4 (IPI with Notch1 Q01705, Notch2 O35516, Notch3 Q61982, Notch4 P31695). Reason: Generic protein binding; the informative MF is Notch binding. Proposed replacements: Notch binding Supporting Evidence: PMID:8749394 RBP-J kappa and Su(H) bind directly to the RAM23 regions of mouse Notch1 and Drosophila Notch, respectively. PMID:9111338 RBP-Jkappa is a sequence-specific DNA binding protein which plays a central role in signalling downstream of the Notch receptor by physically interacting with its intracellular region. |
| GO:0005515 protein binding | IPI PMID:24290140 Structure and function of the CSL-KyoT2 corepressor complex:... | MODIFY | Summary: Rbpj binds corepressors: KyoT2 (Fhl1 isoform), L3MBTL3, and LSD1/KDM1A-containing corepressor complexes. Reason: Generic protein binding; these are corepressor interactions mediating Rbpj repression, best captured as transcription corepressor binding. Proposed replacements: transcription corepressor binding Supporting Evidence: PMID:24290140 our studies provide molecular insights into how KyoT2 binds CSL with high affinity and competes with coactivators, such as Notch, for binding CSL. PMID:29030483 L3MBTL3 competes with NOTCH ICD for binding to RBPJ |
| GO:0005515 protein binding | IPI PMID:29030483 RBPJ/CBF1 interacts with L3MBTL3/MBT1 to promote repression ... | MODIFY | Summary: Rbpj binds corepressors: KyoT2 (Fhl1 isoform), L3MBTL3, and LSD1/KDM1A-containing corepressor complexes. Reason: Generic protein binding; these are corepressor interactions mediating Rbpj repression, best captured as transcription corepressor binding. Proposed replacements: transcription corepressor binding Supporting Evidence: PMID:24290140 our studies provide molecular insights into how KyoT2 binds CSL with high affinity and competes with coactivators, such as Notch, for binding CSL. PMID:29030483 L3MBTL3 competes with NOTCH ICD for binding to RBPJ |
| GO:0005515 protein binding | IPI PMID:8749394 Physical interaction between a novel domain of the receptor ... | MODIFY | Summary: Rbpj binds the intracellular domains of Notch1-4 (IPI with Notch1 Q01705, Notch2 O35516, Notch3 Q61982, Notch4 P31695). Reason: Generic protein binding; the informative MF is Notch binding. Proposed replacements: Notch binding Supporting Evidence: PMID:8749394 RBP-J kappa and Su(H) bind directly to the RAM23 regions of mouse Notch1 and Drosophila Notch, respectively. PMID:9111338 RBP-Jkappa is a sequence-specific DNA binding protein which plays a central role in signalling downstream of the Notch receptor by physically interacting with its intracellular region. |
| GO:0005515 protein binding | IPI PMID:9111338 RBP-L, a transcription factor related to RBP-Jkappa. | MODIFY | Summary: Rbpj binds the intracellular domains of Notch1-4 (IPI with Notch1 Q01705, Notch2 O35516, Notch3 Q61982, Notch4 P31695). Reason: Generic protein binding; the informative MF is Notch binding. Proposed replacements: Notch binding Supporting Evidence: PMID:8749394 RBP-J kappa and Su(H) bind directly to the RAM23 regions of mouse Notch1 and Drosophila Notch, respectively. PMID:9111338 RBP-Jkappa is a sequence-specific DNA binding protein which plays a central role in signalling downstream of the Notch receptor by physically interacting with its intracellular region. |
| GO:0005515 protein binding | IPI PMID:9111338 RBP-L, a transcription factor related to RBP-Jkappa. | MODIFY | Summary: Rbpj binds the intracellular domains of Notch1-4 (IPI with Notch1 Q01705, Notch2 O35516, Notch3 Q61982, Notch4 P31695). Reason: Generic protein binding; the informative MF is Notch binding. Proposed replacements: Notch binding Supporting Evidence: PMID:8749394 RBP-J kappa and Su(H) bind directly to the RAM23 regions of mouse Notch1 and Drosophila Notch, respectively. PMID:9111338 RBP-Jkappa is a sequence-specific DNA binding protein which plays a central role in signalling downstream of the Notch receptor by physically interacting with its intracellular region. |
| GO:0005515 protein binding | IPI PMID:9111338 RBP-L, a transcription factor related to RBP-Jkappa. | MODIFY | Summary: Rbpj binds the intracellular domains of Notch1-4 (IPI with Notch1 Q01705, Notch2 O35516, Notch3 Q61982, Notch4 P31695). Reason: Generic protein binding; the informative MF is Notch binding. Proposed replacements: Notch binding Supporting Evidence: PMID:8749394 RBP-J kappa and Su(H) bind directly to the RAM23 regions of mouse Notch1 and Drosophila Notch, respectively. PMID:9111338 RBP-Jkappa is a sequence-specific DNA binding protein which plays a central role in signalling downstream of the Notch receptor by physically interacting with its intracellular region. |
| GO:0005515 protein binding | IPI PMID:9111338 RBP-L, a transcription factor related to RBP-Jkappa. | MODIFY | Summary: Rbpj binds the intracellular domains of Notch1-4 (IPI with Notch1 Q01705, Notch2 O35516, Notch3 Q61982, Notch4 P31695). Reason: Generic protein binding; the informative MF is Notch binding. Proposed replacements: Notch binding Supporting Evidence: PMID:8749394 RBP-J kappa and Su(H) bind directly to the RAM23 regions of mouse Notch1 and Drosophila Notch, respectively. PMID:9111338 RBP-Jkappa is a sequence-specific DNA binding protein which plays a central role in signalling downstream of the Notch receptor by physically interacting with its intracellular region. |
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005634 nucleus | IC PMID:9111338 RBP-L, a transcription factor related to RBP-Jkappa. | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005634 nucleus | IDA PMID:17079689 Canonical notch signaling functions as a commitment switch i... | ACCEPT | Summary: Nuclear Rbpj in epidermis, mediating NICD/RBP-J target (Hes1) expression. Reason: Rbpj acts on chromatin in the nucleus. Supporting Evidence: PMID:17079689 In contrast, the NICD/RBP-J target gene Hes1 is expressed in spinous layers |
| GO:0005634 nucleus | IDA PMID:17336907 Notch signaling is essential for ventricular chamber develop... | ACCEPT | Summary: Nuclear Rbpj bound with N1ICD at EphrinB2 regulatory elements (ChIP) in developing heart. Reason: Rbpj acts on chromatin in the nucleus. Supporting Evidence: PMID:17336907 Our ChIP assays demonstrated that N1ICD/RBPJK binds to two conserved RBPJK responsive elements within the EphrinB2 genomic DNA |
| GO:0005634 nucleus | IDA PMID:9102301 Conservation of the Notch signalling pathway in mammalian ne... | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005634 nucleus | IEA GO_REF:0000120 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005634 nucleus | ISO GO_REF:0000119 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005634 nucleus | ISO PMID:11741889 HERP1 is a cell type-specific primary target of Notch. | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005634 nucleus | ISS GO_REF:0000024 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005654 nucleoplasm | IEA GO_REF:0000107 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005654 nucleoplasm | ISO GO_REF:0000119 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005654 nucleoplasm | TAS Reactome:R-MMU-2976706 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005654 nucleoplasm | TAS Reactome:R-MMU-2976717 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005654 nucleoplasm | TAS Reactome:R-MMU-2976723 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005654 nucleoplasm | TAS Reactome:R-MMU-4396387 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005654 nucleoplasm | TAS Reactome:R-MMU-9016915 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005654 nucleoplasm | TAS Reactome:R-MMU-9017420 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005654 nucleoplasm | TAS Reactome:R-MMU-9824206 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005654 nucleoplasm | TAS Reactome:R-MMU-9824230 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005654 nucleoplasm | TAS Reactome:R-MMU-9824231 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005654 nucleoplasm | TAS Reactome:R-MMU-9824278 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005654 nucleoplasm | TAS Reactome:R-MMU-9977960 | ACCEPT | Summary: Nuclear/nucleoplasmic localisation of a DNA-binding transcription factor. Reason: Rbpj acts on chromatin in the nucleus. |
| GO:0005667 transcription regulator complex | IDA PMID:17938243 Early pancreatic development requires the vertebrate Suppres... | ACCEPT | Summary: Rbpj is part of transcription regulator complexes: CSL-NICD-MAML activator, CSL-corepressor complexes, and the PTF1-J trimeric DNA-binding complex. Reason: Supported by biochemical evidence for multiple Rbpj-containing DNA-bound complexes. Supporting Evidence: PMID:17938243 active PTF1a requires interaction with RBPJ, the vertebrate Suppressor of Hairless, within a stable trimeric DNA-binding complex (PTF1). |
| GO:0005667 transcription regulator complex | IEA GO_REF:0000117 | ACCEPT | Summary: Rbpj is part of transcription regulator complexes: CSL-NICD-MAML activator, CSL-corepressor complexes, and the PTF1-J trimeric DNA-binding complex. Reason: Supported by biochemical evidence for multiple Rbpj-containing DNA-bound complexes. Supporting Evidence: PMID:17938243 active PTF1a requires interaction with RBPJ, the vertebrate Suppressor of Hairless, within a stable trimeric DNA-binding complex (PTF1). |
| GO:0005730 nucleolus | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Nucleolus, transferred from human RBPJ (likely high-throughput imaging). Reason: Not a known functional site for CSL; retain as a non-core observed location without functional implication. |
| GO:0005730 nucleolus | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: Nucleolus, transferred from human RBPJ (likely high-throughput imaging). Reason: Not a known functional site for CSL; retain as a non-core observed location without functional implication. |
| GO:0005737 cytoplasm | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: Cytoplasm (IEA/ISO/ISS). Reason: Some cytoplasmic pool may exist, but Rbpj functions in the nucleus; cytoplasm is not a site of its core activity. |
| GO:0005737 cytoplasm | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: Cytoplasm (IEA/ISO/ISS). Reason: Some cytoplasmic pool may exist, but Rbpj functions in the nucleus; cytoplasm is not a site of its core activity. |
| GO:0005737 cytoplasm | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Cytoplasm (IEA/ISO/ISS). Reason: Some cytoplasmic pool may exist, but Rbpj functions in the nucleus; cytoplasm is not a site of its core activity. |
| GO:0006355 regulation of DNA-templated transcription | IEA GO_REF:0000002 | ACCEPT | Summary: General regulation of transcription / gene expression. Reason: Correct but general; more specific positive and negative Pol II regulation terms apply. |
| GO:0006357 regulation of transcription by RNA polymerase II | IEA GO_REF:0000002 | ACCEPT | Summary: General regulation of transcription / gene expression. Reason: Correct but general; more specific positive and negative Pol II regulation terms apply. |
| GO:0006357 regulation of transcription by RNA polymerase II | IGI PMID:15689374 RBPjkappa-dependent Notch function regulates Gata2 and is es... | ACCEPT | Summary: General regulation of transcription / gene expression. Reason: Correct but general; more specific positive and negative Pol II regulation terms apply. |
| GO:0006357 regulation of transcription by RNA polymerase II | IMP PMID:21311046 Differential Notch signaling in the epicardium is required f... | ACCEPT | Summary: General regulation of transcription / gene expression. Reason: Correct but general; more specific positive and negative Pol II regulation terms apply. |
| GO:0006959 humoral immune response | IMP PMID:17658279 Direct regulation of Gata3 expression determines the T helpe... | KEEP AS NON CORE | Summary: Immune/haematopoietic phenotypes of Rbpj conditional loss (marginal zone B cells, Th2/Gata3, dendritic cells, Gata2-dependent haematopoiesis). Reason: Consequences of loss of Notch-RBP-J transcriptional control in specific immune lineages; pleiotropic, non-core. |
| GO:0007219 Notch signaling pathway | IBA GO_REF:0000033 | ACCEPT | Summary: Rbpj is the obligate nuclear effector of canonical Notch signalling; its loss phenocopies Notch1 loss and abolishes Notch-dependent transcription. Reason: Core biological process. Supporting Evidence: PMID:8749394 in the mouse, the phenotypes of homozygous mutant Notch1 embryos are very similar to those of homozygous mutant RBP-J kappa embryos. PMID:16287852 the intracellular part of Notch translocates to the nucleus, where it targets the DNA-binding protein RBP-Jkappa/CBF1. file:mouse/Rbpj/Rbpj-deep-research-falcon.md Rbpj encodes a sequence-specific DNA-binding transcription factor that serves as the central nuclear mediator of canonical Notch signaling in mammals |
| GO:0007219 Notch signaling pathway | IEA GO_REF:0000107 | ACCEPT | Summary: Rbpj is the obligate nuclear effector of canonical Notch signalling; its loss phenocopies Notch1 loss and abolishes Notch-dependent transcription. Reason: Core biological process. Supporting Evidence: PMID:8749394 in the mouse, the phenotypes of homozygous mutant Notch1 embryos are very similar to those of homozygous mutant RBP-J kappa embryos. PMID:16287852 the intracellular part of Notch translocates to the nucleus, where it targets the DNA-binding protein RBP-Jkappa/CBF1. file:mouse/Rbpj/Rbpj-deep-research-falcon.md Rbpj encodes a sequence-specific DNA-binding transcription factor that serves as the central nuclear mediator of canonical Notch signaling in mammals |
| GO:0007219 Notch signaling pathway | IGI PMID:25406395 RBPJ in mouse Sertoli cells is required for proper regulatio... | ACCEPT | Summary: Rbpj is the obligate nuclear effector of canonical Notch signalling; its loss phenocopies Notch1 loss and abolishes Notch-dependent transcription. Reason: Core biological process. Supporting Evidence: PMID:8749394 in the mouse, the phenotypes of homozygous mutant Notch1 embryos are very similar to those of homozygous mutant RBP-J kappa embryos. PMID:16287852 the intracellular part of Notch translocates to the nucleus, where it targets the DNA-binding protein RBP-Jkappa/CBF1. file:mouse/Rbpj/Rbpj-deep-research-falcon.md Rbpj encodes a sequence-specific DNA-binding transcription factor that serves as the central nuclear mediator of canonical Notch signaling in mammals |
| GO:0007219 Notch signaling pathway | IMP PMID:14701881 Notch promotes epithelial-mesenchymal transition during card... | ACCEPT | Summary: Rbpj is the obligate nuclear effector of canonical Notch signalling; its loss phenocopies Notch1 loss and abolishes Notch-dependent transcription. Reason: Core biological process. Supporting Evidence: PMID:8749394 in the mouse, the phenotypes of homozygous mutant Notch1 embryos are very similar to those of homozygous mutant RBP-J kappa embryos. PMID:16287852 the intracellular part of Notch translocates to the nucleus, where it targets the DNA-binding protein RBP-Jkappa/CBF1. file:mouse/Rbpj/Rbpj-deep-research-falcon.md Rbpj encodes a sequence-specific DNA-binding transcription factor that serves as the central nuclear mediator of canonical Notch signaling in mammals |
| GO:0007219 Notch signaling pathway | IMP PMID:15466160 Haploinsufficient lethality and formation of arteriovenous m... | ACCEPT | Summary: Rbpj is the obligate nuclear effector of canonical Notch signalling; its loss phenocopies Notch1 loss and abolishes Notch-dependent transcription. Reason: Core biological process. Supporting Evidence: PMID:8749394 in the mouse, the phenotypes of homozygous mutant Notch1 embryos are very similar to those of homozygous mutant RBP-J kappa embryos. PMID:16287852 the intracellular part of Notch translocates to the nucleus, where it targets the DNA-binding protein RBP-Jkappa/CBF1. file:mouse/Rbpj/Rbpj-deep-research-falcon.md Rbpj encodes a sequence-specific DNA-binding transcription factor that serves as the central nuclear mediator of canonical Notch signaling in mammals |
| GO:0007219 Notch signaling pathway | IMP PMID:19124651 Progenitor cell proliferation in the retina is dependent on ... | ACCEPT | Summary: Rbpj is the obligate nuclear effector of canonical Notch signalling; its loss phenocopies Notch1 loss and abolishes Notch-dependent transcription. Reason: Core biological process. Supporting Evidence: PMID:8749394 in the mouse, the phenotypes of homozygous mutant Notch1 embryos are very similar to those of homozygous mutant RBP-J kappa embryos. PMID:16287852 the intracellular part of Notch translocates to the nucleus, where it targets the DNA-binding protein RBP-Jkappa/CBF1. file:mouse/Rbpj/Rbpj-deep-research-falcon.md Rbpj encodes a sequence-specific DNA-binding transcription factor that serves as the central nuclear mediator of canonical Notch signaling in mammals |
| GO:0007219 Notch signaling pathway | IMP PMID:19154718 Hey2 regulation by FGF provides a Notch-independent mechanis... | ACCEPT | Summary: Rbpj is the obligate nuclear effector of canonical Notch signalling; its loss phenocopies Notch1 loss and abolishes Notch-dependent transcription. Reason: Core biological process. Supporting Evidence: PMID:8749394 in the mouse, the phenotypes of homozygous mutant Notch1 embryos are very similar to those of homozygous mutant RBP-J kappa embryos. PMID:16287852 the intracellular part of Notch translocates to the nucleus, where it targets the DNA-binding protein RBP-Jkappa/CBF1. file:mouse/Rbpj/Rbpj-deep-research-falcon.md Rbpj encodes a sequence-specific DNA-binding transcription factor that serves as the central nuclear mediator of canonical Notch signaling in mammals |
| GO:0007219 Notch signaling pathway | IMP PMID:21311046 Differential Notch signaling in the epicardium is required f... | ACCEPT | Summary: Rbpj is the obligate nuclear effector of canonical Notch signalling; its loss phenocopies Notch1 loss and abolishes Notch-dependent transcription. Reason: Core biological process. Supporting Evidence: PMID:8749394 in the mouse, the phenotypes of homozygous mutant Notch1 embryos are very similar to those of homozygous mutant RBP-J kappa embryos. PMID:16287852 the intracellular part of Notch translocates to the nucleus, where it targets the DNA-binding protein RBP-Jkappa/CBF1. file:mouse/Rbpj/Rbpj-deep-research-falcon.md Rbpj encodes a sequence-specific DNA-binding transcription factor that serves as the central nuclear mediator of canonical Notch signaling in mammals |
| GO:0007219 Notch signaling pathway | IMP PMID:25406395 RBPJ in mouse Sertoli cells is required for proper regulatio... | ACCEPT | Summary: Rbpj is the obligate nuclear effector of canonical Notch signalling; its loss phenocopies Notch1 loss and abolishes Notch-dependent transcription. Reason: Core biological process. Supporting Evidence: PMID:8749394 in the mouse, the phenotypes of homozygous mutant Notch1 embryos are very similar to those of homozygous mutant RBP-J kappa embryos. PMID:16287852 the intracellular part of Notch translocates to the nucleus, where it targets the DNA-binding protein RBP-Jkappa/CBF1. file:mouse/Rbpj/Rbpj-deep-research-falcon.md Rbpj encodes a sequence-specific DNA-binding transcription factor that serves as the central nuclear mediator of canonical Notch signaling in mammals |
| GO:0007219 Notch signaling pathway | ISO GO_REF:0000119 | ACCEPT | Summary: Rbpj is the obligate nuclear effector of canonical Notch signalling; its loss phenocopies Notch1 loss and abolishes Notch-dependent transcription. Reason: Core biological process. Supporting Evidence: PMID:8749394 in the mouse, the phenotypes of homozygous mutant Notch1 embryos are very similar to those of homozygous mutant RBP-J kappa embryos. PMID:16287852 the intracellular part of Notch translocates to the nucleus, where it targets the DNA-binding protein RBP-Jkappa/CBF1. file:mouse/Rbpj/Rbpj-deep-research-falcon.md Rbpj encodes a sequence-specific DNA-binding transcription factor that serves as the central nuclear mediator of canonical Notch signaling in mammals |
| GO:0007219 Notch signaling pathway | TAS PMID:15466160 Haploinsufficient lethality and formation of arteriovenous m... | ACCEPT | Summary: Rbpj is the obligate nuclear effector of canonical Notch signalling; its loss phenocopies Notch1 loss and abolishes Notch-dependent transcription. Reason: Core biological process. Supporting Evidence: PMID:8749394 in the mouse, the phenotypes of homozygous mutant Notch1 embryos are very similar to those of homozygous mutant RBP-J kappa embryos. PMID:16287852 the intracellular part of Notch translocates to the nucleus, where it targets the DNA-binding protein RBP-Jkappa/CBF1. file:mouse/Rbpj/Rbpj-deep-research-falcon.md Rbpj encodes a sequence-specific DNA-binding transcription factor that serves as the central nuclear mediator of canonical Notch signaling in mammals |
| GO:0007221 positive regulation of transcription of Notch receptor target | IEA GO_REF:0000120 | ACCEPT | Summary: Positive regulation of transcription of Notch receptor target. Reason: Precisely describes Rbpj's role as the DNA-binding component that activates Notch target genes together with NICD and MAML. Supporting Evidence: PMID:12730124 Analysis of the enhancer regulating node-specific Nodal expression revealed the presence of binding sites for the RBP-J protein, the primary transcriptional mediator of Notch signaling. |
| GO:0007221 positive regulation of transcription of Notch receptor target | IGI PMID:12794108 Notch-regulated ankyrin-repeat protein inhibits Notch1 signa... | ACCEPT | Summary: Positive regulation of transcription of Notch receptor target. Reason: Precisely describes Rbpj's role as the DNA-binding component that activates Notch target genes together with NICD and MAML. Supporting Evidence: PMID:12730124 Analysis of the enhancer regulating node-specific Nodal expression revealed the presence of binding sites for the RBP-J protein, the primary transcriptional mediator of Notch signaling. |
| GO:0007221 positive regulation of transcription of Notch receptor target | ISO GO_REF:0000119 | ACCEPT | Summary: Positive regulation of transcription of Notch receptor target. Reason: Precisely describes Rbpj's role as the DNA-binding component that activates Notch target genes together with NICD and MAML. Supporting Evidence: PMID:12730124 Analysis of the enhancer regulating node-specific Nodal expression revealed the presence of binding sites for the RBP-J protein, the primary transcriptional mediator of Notch signaling. |
| GO:0007221 positive regulation of transcription of Notch receptor target | ISS GO_REF:0000024 | ACCEPT | Summary: Positive regulation of transcription of Notch receptor target. Reason: Precisely describes Rbpj's role as the DNA-binding component that activates Notch target genes together with NICD and MAML. Supporting Evidence: PMID:12730124 Analysis of the enhancer regulating node-specific Nodal expression revealed the presence of binding sites for the RBP-J protein, the primary transcriptional mediator of Notch signaling. |
| GO:0007507 heart development | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0007507 heart development | IMP PMID:14701881 Notch promotes epithelial-mesenchymal transition during card... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0007507 heart development | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0008284 positive regulation of cell population proliferation | IGI PMID:15509736 Fibroblast growth factor receptor signaling promotes radial ... | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0008284 positive regulation of cell population proliferation | IGI PMID:15509736 Fibroblast growth factor receptor signaling promotes radial ... | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0008285 negative regulation of cell population proliferation | IMP PMID:17079689 Canonical notch signaling functions as a commitment switch i... | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0009912 auditory receptor cell fate commitment | IMP PMID:19154718 Hey2 regulation by FGF provides a Notch-independent mechanis... | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0009957 epidermal cell fate specification | IMP PMID:17079689 Canonical notch signaling functions as a commitment switch i... | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0010468 regulation of gene expression | IMP PMID:9102301 Conservation of the Notch signalling pathway in mammalian ne... | ACCEPT | Summary: General regulation of transcription / gene expression. Reason: Correct but general; more specific positive and negative Pol II regulation terms apply. |
| GO:0010628 positive regulation of gene expression | IMP PMID:23117660 Transcriptome analysis for Notch3 target genes identifies Gr... | ACCEPT | Summary: Smooth-muscle RBP-Jkappa is required for expression of Notch3 target genes. Reason: Consistent with Rbpj as the transcriptional effector of Notch3 signalling. Supporting Evidence: PMID:23117660 We first investigated the requirement of smooth muscle RBP-JΞΊ for the expression of Notch3 target genes. |
| GO:0010977 negative regulation of neuron projection development | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: ISO from rat Rbpj for neuron projection and smooth muscle cell behaviours. Reason: Context-specific outcomes of Notch-RBP-J signalling; non-core. |
| GO:0014912 negative regulation of smooth muscle cell migration | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: ISO from rat Rbpj for neuron projection and smooth muscle cell behaviours. Reason: Context-specific outcomes of Notch-RBP-J signalling; non-core. |
| GO:0017053 transcription repressor complex | IEA GO_REF:0000107 | ACCEPT | Summary: Rbpj is part of transcription regulator complexes: CSL-NICD-MAML activator, CSL-corepressor complexes, and the PTF1-J trimeric DNA-binding complex. Reason: Supported by biochemical evidence for multiple Rbpj-containing DNA-bound complexes. Supporting Evidence: PMID:17938243 active PTF1a requires interaction with RBPJ, the vertebrate Suppressor of Hairless, within a stable trimeric DNA-binding complex (PTF1). |
| GO:0017053 transcription repressor complex | ISO GO_REF:0000119 | ACCEPT | Summary: Rbpj is part of transcription regulator complexes: CSL-NICD-MAML activator, CSL-corepressor complexes, and the PTF1-J trimeric DNA-binding complex. Reason: Supported by biochemical evidence for multiple Rbpj-containing DNA-bound complexes. Supporting Evidence: PMID:17938243 active PTF1a requires interaction with RBPJ, the vertebrate Suppressor of Hairless, within a stable trimeric DNA-binding complex (PTF1). |
| GO:0021983 pituitary gland development | IMP PMID:17015435 Sustained Notch signaling in progenitors is required for seq... | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0030097 hemopoiesis | IMP PMID:15689374 RBPjkappa-dependent Notch function regulates Gata2 and is es... | KEEP AS NON CORE | Summary: Immune/haematopoietic phenotypes of Rbpj conditional loss (marginal zone B cells, Th2/Gata3, dendritic cells, Gata2-dependent haematopoiesis). Reason: Consequences of loss of Notch-RBP-J transcriptional control in specific immune lineages; pleiotropic, non-core. |
| GO:0030182 neuron differentiation | IMP PMID:9102301 Conservation of the Notch signalling pathway in mammalian ne... | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0030183 B cell differentiation | IMP PMID:11967543 Notch-RBP-J signaling is involved in cell fate determination... | KEEP AS NON CORE | Summary: Immune/haematopoietic phenotypes of Rbpj conditional loss (marginal zone B cells, Th2/Gata3, dendritic cells, Gata2-dependent haematopoiesis). Reason: Consequences of loss of Notch-RBP-J transcriptional control in specific immune lineages; pleiotropic, non-core. |
| GO:0030216 keratinocyte differentiation | IMP PMID:17079689 Canonical notch signaling functions as a commitment switch i... | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0030279 negative regulation of ossification | IMP PMID:21493891 Diet-induced aortic valve disease in mice haploinsufficient ... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0030513 positive regulation of BMP signaling pathway | IMP PMID:17336907 Notch signaling is essential for ventricular chamber develop... | KEEP AS NON CORE | Summary: Rbpj loss reduces expression of signalling components (e.g. Bmp10, Nrg1/ErbB, ephrinB2, Wnt pathway targets) in the developing heart, altering those pathways. Reason: Indirect, transcriptional cross-regulation of other pathways via Notch targets; not a direct role of Rbpj in those signalling pathways. |
| GO:0032991 protein-containing complex | ISO GO_REF:0000096 | ACCEPT | Summary: Rbpj is part of transcription regulator complexes: CSL-NICD-MAML activator, CSL-corepressor complexes, and the PTF1-J trimeric DNA-binding complex. Reason: Supported by biochemical evidence for multiple Rbpj-containing DNA-bound complexes. Supporting Evidence: PMID:17938243 active PTF1a requires interaction with RBPJ, the vertebrate Suppressor of Hairless, within a stable trimeric DNA-binding complex (PTF1). |
| GO:0032993 protein-DNA complex | ISO GO_REF:0000096 | ACCEPT | Summary: Rbpj is part of transcription regulator complexes: CSL-NICD-MAML activator, CSL-corepressor complexes, and the PTF1-J trimeric DNA-binding complex. Reason: Supported by biochemical evidence for multiple Rbpj-containing DNA-bound complexes. Supporting Evidence: PMID:17938243 active PTF1a requires interaction with RBPJ, the vertebrate Suppressor of Hairless, within a stable trimeric DNA-binding complex (PTF1). |
| GO:0034392 negative regulation of smooth muscle cell apoptotic process | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: ISO from rat Rbpj for neuron projection and smooth muscle cell behaviours. Reason: Context-specific outcomes of Notch-RBP-J signalling; non-core. |
| GO:0035019 somatic stem cell population maintenance | IMP PMID:21402740 Notch signaling in stomach epithelial stem cell homeostasis. | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0035912 dorsal aorta morphogenesis | IMP PMID:15466160 Haploinsufficient lethality and formation of arteriovenous m... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0036302 atrioventricular canal development | IMP PMID:14701881 Notch promotes epithelial-mesenchymal transition during card... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0042127 regulation of cell population proliferation | IGI PMID:25406395 RBPJ in mouse Sertoli cells is required for proper regulatio... | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0042742 defense response to bacterium | IMP PMID:11967543 Notch-RBP-J signaling is involved in cell fate determination... | KEEP AS NON CORE | Summary: Immune/haematopoietic phenotypes of Rbpj conditional loss (marginal zone B cells, Th2/Gata3, dendritic cells, Gata2-dependent haematopoiesis). Reason: Consequences of loss of Notch-RBP-J transcriptional control in specific immune lineages; pleiotropic, non-core. |
| GO:0043011 myeloid dendritic cell differentiation | IMP PMID:23913046 Notch2-dependent classical dendritic cells orchestrate intes... | KEEP AS NON CORE | Summary: Immune/haematopoietic phenotypes of Rbpj conditional loss (marginal zone B cells, Th2/Gata3, dendritic cells, Gata2-dependent haematopoiesis). Reason: Consequences of loss of Notch-RBP-J transcriptional control in specific immune lineages; pleiotropic, non-core. |
| GO:0043467 regulation of generation of precursor metabolites and energy | IEA GO_REF:0000107 | MARK AS OVER ANNOTATED | Summary: Orthology transfer from human RBPJ (Q06330), whose source is an IDA (PMID:23303788) showing RBPJ binds the oxygen-responsive element of COX4I2 and activates its transcription at 4% oxygen. Reason: The underlying experiment shows transcriptional activation of a single gene (COX4I2); a process term about generation of precursor metabolites and energy overstates it. Harmonised with the human RBPJ review, which marks the source IDA as over-annotated for the same reason. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: GRANULARITY MISMATCH Sources checked: UniProtKB:Q06330 Β· RBPJ SOURCE WEAK OR INFERRED Human source IDA (PMID:23303788) shows activation of one gene (COX4I2); the energy-metabolism process term overstates it in both source and target. Supporting Evidence: PMID:23303788 We demonstrate that RBPJ and CHCHD2 function towards activating the ORE at 4% oxygen, |
| GO:0043467 regulation of generation of precursor metabolites and energy | ISO GO_REF:0000119 | MARK AS OVER ANNOTATED | Summary: Orthology transfer from human RBPJ (Q06330), whose source is an IDA (PMID:23303788) showing RBPJ binds the oxygen-responsive element of COX4I2 and activates its transcription at 4% oxygen. Reason: The underlying experiment shows transcriptional activation of a single gene (COX4I2); a process term about generation of precursor metabolites and energy overstates it. Harmonised with the human RBPJ review, which marks the source IDA as over-annotated for the same reason. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: GRANULARITY MISMATCH Sources checked: UniProtKB:Q06330 Β· RBPJ SOURCE WEAK OR INFERRED Human source IDA (PMID:23303788) shows activation of one gene (COX4I2); the energy-metabolism process term overstates it in both source and target. Supporting Evidence: PMID:23303788 We demonstrate that RBPJ and CHCHD2 function towards activating the ORE at 4% oxygen, |
| GO:0043565 sequence-specific DNA binding | IDA PMID:17658278 Notch directly regulates Gata3 expression during T helper 2 ... | ACCEPT | Summary: Rbpj is a sequence-specific DNA-binding protein recognising the CGTGGGAA (CSL) motif in Pol II promoters/enhancers of Notch targets and PTF1 targets. Reason: Sequence-specific DNA binding is the defining molecular property of CSL proteins. Supporting Evidence: PMID:8749394 The mammalian transcription factor RBP-J kappa binds to the DNA sequence motif CGTGGGAA and is involved in the regulation of gene expression |
| GO:0043565 sequence-specific DNA binding | IDA PMID:17938243 Early pancreatic development requires the vertebrate Suppres... | ACCEPT | Summary: Rbpj is a sequence-specific DNA-binding protein recognising the CGTGGGAA (CSL) motif in Pol II promoters/enhancers of Notch targets and PTF1 targets. Reason: Sequence-specific DNA binding is the defining molecular property of CSL proteins. Supporting Evidence: PMID:8749394 The mammalian transcription factor RBP-J kappa binds to the DNA sequence motif CGTGGGAA and is involved in the regulation of gene expression |
| GO:0043565 sequence-specific DNA binding | IDA PMID:18381292 RAM-induced allostery facilitates assembly of a notch pathwa... | ACCEPT | Summary: Rbpj is a sequence-specific DNA-binding protein recognising the CGTGGGAA (CSL) motif in Pol II promoters/enhancers of Notch targets and PTF1 targets. Reason: Sequence-specific DNA binding is the defining molecular property of CSL proteins. Supporting Evidence: PMID:8749394 The mammalian transcription factor RBP-J kappa binds to the DNA sequence motif CGTGGGAA and is involved in the regulation of gene expression |
| GO:0043565 sequence-specific DNA binding | IEA GO_REF:0000107 | ACCEPT | Summary: Rbpj is a sequence-specific DNA-binding protein recognising the CGTGGGAA (CSL) motif in Pol II promoters/enhancers of Notch targets and PTF1 targets. Reason: Sequence-specific DNA binding is the defining molecular property of CSL proteins. Supporting Evidence: PMID:8749394 The mammalian transcription factor RBP-J kappa binds to the DNA sequence motif CGTGGGAA and is involved in the regulation of gene expression |
| GO:0043565 sequence-specific DNA binding | ISO GO_REF:0000119 | ACCEPT | Summary: Rbpj is a sequence-specific DNA-binding protein recognising the CGTGGGAA (CSL) motif in Pol II promoters/enhancers of Notch targets and PTF1 targets. Reason: Sequence-specific DNA binding is the defining molecular property of CSL proteins. Supporting Evidence: PMID:8749394 The mammalian transcription factor RBP-J kappa binds to the DNA sequence motif CGTGGGAA and is involved in the regulation of gene expression |
| GO:0044877 protein-containing complex binding | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: Protein-containing complex binding (ISO from rat). Reason: Vague; the informative interactions are Notch binding and corepressor/TF binding. |
| GO:0045165 cell fate commitment | IMP PMID:17015435 Sustained Notch signaling in progenitors is required for seq... | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0045596 negative regulation of cell differentiation | IMP PMID:10476967 Notch signalling controls pancreatic cell differentiation. | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0045596 negative regulation of cell differentiation | IMP PMID:17015435 Sustained Notch signaling in progenitors is required for seq... | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0045892 negative regulation of DNA-templated transcription | IDA PMID:9111338 RBP-L, a transcription factor related to RBP-Jkappa. | ACCEPT | Summary: Rbpj represses Notch target genes in the absence of NICD by recruiting corepressor complexes (SHARP/CtIP/CtBP, KyoT2, L3MBTL3-KDM1A). Reason: Repression is one half of the core CSL switch function. Supporting Evidence: PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. |
| GO:0045892 negative regulation of DNA-templated transcription | IEA GO_REF:0000107 | ACCEPT | Summary: Rbpj represses Notch target genes in the absence of NICD by recruiting corepressor complexes (SHARP/CtIP/CtBP, KyoT2, L3MBTL3-KDM1A). Reason: Repression is one half of the core CSL switch function. Supporting Evidence: PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. |
| GO:0045892 negative regulation of DNA-templated transcription | ISO GO_REF:0000119 | ACCEPT | Summary: Rbpj represses Notch target genes in the absence of NICD by recruiting corepressor complexes (SHARP/CtIP/CtBP, KyoT2, L3MBTL3-KDM1A). Reason: Repression is one half of the core CSL switch function. Supporting Evidence: PMID:16287852 In the absence of Notch, RBP-Jkappa represses Notch target genes through the recruitment of a corepressor complex. |
| GO:0045893 positive regulation of DNA-templated transcription | IEA GO_REF:0000107 | ACCEPT | Summary: Rbpj activates target gene transcription as part of the CSL-NICD-MAML complex. Reason: Activation of Notch target genes is the core transcriptional output of Rbpj. Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0045893 positive regulation of DNA-templated transcription | ISO GO_REF:0000096 | ACCEPT | Summary: Rbpj activates target gene transcription as part of the CSL-NICD-MAML complex. Reason: Activation of Notch target genes is the core transcriptional output of Rbpj. Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0045893 positive regulation of DNA-templated transcription | ISO GO_REF:0000119 | ACCEPT | Summary: Rbpj activates target gene transcription as part of the CSL-NICD-MAML complex. Reason: Activation of Notch target genes is the core transcriptional output of Rbpj. Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0045893 positive regulation of DNA-templated transcription | ISO PMID:11741889 HERP1 is a cell type-specific primary target of Notch. | ACCEPT | Summary: Rbpj activates target gene transcription as part of the CSL-NICD-MAML complex. Reason: Activation of Notch target genes is the core transcriptional output of Rbpj. Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IDA PMID:12730124 Notch signaling regulates left-right asymmetry determination... | ACCEPT | Summary: Rbpj activates target gene transcription as part of the CSL-NICD-MAML complex. Reason: Activation of Notch target genes is the core transcriptional output of Rbpj. Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IDA PMID:16287852 RBP-Jkappa/SHARP recruits CtIP/CtBP corepressors to silence ... | ACCEPT | Summary: Rbpj activates target gene transcription as part of the CSL-NICD-MAML complex. Reason: Activation of Notch target genes is the core transcriptional output of Rbpj. Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IEA GO_REF:0000107 | ACCEPT | Summary: Rbpj activates target gene transcription as part of the CSL-NICD-MAML complex. Reason: Activation of Notch target genes is the core transcriptional output of Rbpj. Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IMP PMID:14701881 Notch promotes epithelial-mesenchymal transition during card... | ACCEPT | Summary: Rbpj activates target gene transcription as part of the CSL-NICD-MAML complex. Reason: Activation of Notch target genes is the core transcriptional output of Rbpj. Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IMP PMID:15466160 Haploinsufficient lethality and formation of arteriovenous m... | ACCEPT | Summary: Rbpj activates target gene transcription as part of the CSL-NICD-MAML complex. Reason: Activation of Notch target genes is the core transcriptional output of Rbpj. Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IMP PMID:17336907 Notch signaling is essential for ventricular chamber develop... | ACCEPT | Summary: Rbpj activates target gene transcription as part of the CSL-NICD-MAML complex. Reason: Activation of Notch target genes is the core transcriptional output of Rbpj. Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IMP PMID:17685488 Monitoring Notch1 activity in development: evidence for a fe... | ACCEPT | Summary: Rbpj activates target gene transcription as part of the CSL-NICD-MAML complex. Reason: Activation of Notch target genes is the core transcriptional output of Rbpj. Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IMP PMID:20890042 Integration of a Notch-dependent mesenchymal gene program an... | ACCEPT | Summary: Rbpj activates target gene transcription as part of the CSL-NICD-MAML complex. Reason: Activation of Notch target genes is the core transcriptional output of Rbpj. Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IMP PMID:21311046 Differential Notch signaling in the epicardium is required f... | ACCEPT | Summary: Rbpj activates target gene transcription as part of the CSL-NICD-MAML complex. Reason: Activation of Notch target genes is the core transcriptional output of Rbpj. Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | IMP PMID:26491108 Notch-Tnf signalling is required for development and homeost... | ACCEPT | Summary: Rbpj activates target gene transcription as part of the CSL-NICD-MAML complex. Reason: Activation of Notch target genes is the core transcriptional output of Rbpj. Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | ISO GO_REF:0000119 | ACCEPT | Summary: Rbpj activates target gene transcription as part of the CSL-NICD-MAML complex. Reason: Activation of Notch target genes is the core transcriptional output of Rbpj. Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0045944 positive regulation of transcription by RNA polymerase II | ISS GO_REF:0000024 | ACCEPT | Summary: Rbpj activates target gene transcription as part of the CSL-NICD-MAML complex. Reason: Activation of Notch target genes is the core transcriptional output of Rbpj. Supporting Evidence: PMID:18381292 CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. |
| GO:0048505 regulation of timing of cell differentiation | IMP PMID:17015435 Sustained Notch signaling in progenitors is required for seq... | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0048513 animal organ development | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0048661 positive regulation of smooth muscle cell proliferation | ISO GO_REF:0000096 | KEEP AS NON CORE | Summary: ISO from rat Rbpj for neuron projection and smooth muscle cell behaviours. Reason: Context-specific outcomes of Notch-RBP-J signalling; non-core. |
| GO:0048733 sebaceous gland development | IMP PMID:17079689 Canonical notch signaling functions as a commitment switch i... | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0048820 hair follicle maturation | IMP PMID:17079689 Canonical notch signaling functions as a commitment switch i... | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0048844 artery morphogenesis | IMP PMID:23117660 Transcriptome analysis for Notch3 target genes identifies Gr... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0060045 positive regulation of cardiac muscle cell proliferation | IMP PMID:17336907 Notch signaling is essential for ventricular chamber develop... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0060379 cardiac muscle cell myoblast differentiation | IMP PMID:21311046 Differential Notch signaling in the epicardium is required f... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0060412 ventricular septum morphogenesis | IMP PMID:26491108 Notch-Tnf signalling is required for development and homeost... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0060486 club cell differentiation | IMP PMID:22797898 Neuroepithelial body microenvironment is a niche for a disti... | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0060716 labyrinthine layer blood vessel development | IMP PMID:15466160 Haploinsufficient lethality and formation of arteriovenous m... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0060844 arterial endothelial cell fate commitment | IMP PMID:15466160 Haploinsufficient lethality and formation of arteriovenous m... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0061344 regulation of cell adhesion involved in heart morphogenesis | IMP PMID:14701881 Notch promotes epithelial-mesenchymal transition during card... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0061371 determination of heart left/right asymmetry | TAS PMID:15466160 Haploinsufficient lethality and formation of arteriovenous m... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: DNA-binding transcription factor binding; Rbpj binds the bHLH factor Ptf1a (p48) in the PTF1-J complex. Reason: Orthology transfer from human RBPJ (Q06330). The human source rows are SPEN/SHARP binding (PMID:16287852), which the human RBPJ review MODIFYs to transcription corepressor binding because SPEN is a corepressor rather than a DNA-binding factor, and two partner/co-binding reports it keeps as non-core. The term is nevertheless independently true for mouse Rbpj, which binds the bHLH transcription factor Ptf1a (p48) in the PTF1-J complex (PMID:11318877). Retained as non-core, consistent with the human review: Ptf1a partnering is a tissue-specific (pancreas, neural tube) function alongside the core Notch-dependent CSL activity. Supporting Evidence: PMID:11318877 We isolated PTF1 p48 from 9.5-day mouse embryos as a binding protein of RBP-Jkappa, a mediator of Notch signalling. |
| GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: DNA-binding transcription factor binding; Rbpj binds the bHLH factor Ptf1a (p48) in the PTF1-J complex. Reason: Orthology transfer from human RBPJ (Q06330). The human source rows are SPEN/SHARP binding (PMID:16287852), which the human RBPJ review MODIFYs to transcription corepressor binding because SPEN is a corepressor rather than a DNA-binding factor, and two partner/co-binding reports it keeps as non-core. The term is nevertheless independently true for mouse Rbpj, which binds the bHLH transcription factor Ptf1a (p48) in the PTF1-J complex (PMID:11318877). Retained as non-core, consistent with the human review: Ptf1a partnering is a tissue-specific (pancreas, neural tube) function alongside the core Notch-dependent CSL activity. Supporting Evidence: PMID:11318877 We isolated PTF1 p48 from 9.5-day mouse embryos as a binding protein of RBP-Jkappa, a mediator of Notch signalling. |
| GO:0072554 blood vessel lumenization | IMP PMID:15466160 Haploinsufficient lethality and formation of arteriovenous m... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0090263 positive regulation of canonical Wnt signaling pathway | IMP PMID:22711842 Wnt/Ξ²-catenin and Bmp signals control distinct sets of trans... | KEEP AS NON CORE | Summary: Rbpj loss reduces expression of signalling components (e.g. Bmp10, Nrg1/ErbB, ephrinB2, Wnt pathway targets) in the developing heart, altering those pathways. Reason: Indirect, transcriptional cross-regulation of other pathways via Notch targets; not a direct role of Rbpj in those signalling pathways. |
| GO:0097101 blood vessel endothelial cell fate specification | IGI PMID:15466160 Haploinsufficient lethality and formation of arteriovenous m... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
| GO:0120163 negative regulation of cold-induced thermogenesis | IMP PMID:25038826 Inhibition of Notch signaling promotes browning of white adi... | KEEP AS NON CORE | Summary: Tissue-specific developmental, proliferation, differentiation or physiological phenotypes of Rbpj loss-of-function (epidermis, hair follicle, pituitary, inner ear, neurons, lung club cells, stomach stem cells, pancreas, testis, adipose, metabolism). Reason: Reflect the pleiotropic requirement for canonical Notch signalling in cell-fate and proliferation decisions; downstream of Rbpj's core transcriptional function. |
| GO:0140297 DNA-binding transcription factor binding | IEA GO_REF:0000107 | KEEP AS NON CORE | Summary: DNA-binding transcription factor binding; Rbpj binds the bHLH factor Ptf1a (p48) in the PTF1-J complex. Reason: Orthology transfer from human RBPJ (Q06330). The human source rows are SPEN/SHARP binding (PMID:16287852), which the human RBPJ review MODIFYs to transcription corepressor binding because SPEN is a corepressor rather than a DNA-binding factor, and two partner/co-binding reports it keeps as non-core. The term is nevertheless independently true for mouse Rbpj, which binds the bHLH transcription factor Ptf1a (p48) in the PTF1-J complex (PMID:11318877). Retained as non-core, consistent with the human review: Ptf1a partnering is a tissue-specific (pancreas, neural tube) function alongside the core Notch-dependent CSL activity. Supporting Evidence: PMID:11318877 We isolated PTF1 p48 from 9.5-day mouse embryos as a binding protein of RBP-Jkappa, a mediator of Notch signalling. |
| GO:0140297 DNA-binding transcription factor binding | ISO GO_REF:0000119 | KEEP AS NON CORE | Summary: DNA-binding transcription factor binding; Rbpj binds the bHLH factor Ptf1a (p48) in the PTF1-J complex. Reason: Orthology transfer from human RBPJ (Q06330). The human source rows are SPEN/SHARP binding (PMID:16287852), which the human RBPJ review MODIFYs to transcription corepressor binding because SPEN is a corepressor rather than a DNA-binding factor, and two partner/co-binding reports it keeps as non-core. The term is nevertheless independently true for mouse Rbpj, which binds the bHLH transcription factor Ptf1a (p48) in the PTF1-J complex (PMID:11318877). Retained as non-core, consistent with the human review: Ptf1a partnering is a tissue-specific (pancreas, neural tube) function alongside the core Notch-dependent CSL activity. Supporting Evidence: PMID:11318877 We isolated PTF1 p48 from 9.5-day mouse embryos as a binding protein of RBP-Jkappa, a mediator of Notch signalling. |
| GO:1901186 positive regulation of ERBB signaling pathway | IMP PMID:17336907 Notch signaling is essential for ventricular chamber develop... | KEEP AS NON CORE | Summary: Rbpj loss reduces expression of signalling components (e.g. Bmp10, Nrg1/ErbB, ephrinB2, Wnt pathway targets) in the developing heart, altering those pathways. Reason: Indirect, transcriptional cross-regulation of other pathways via Notch targets; not a direct role of Rbpj in those signalling pathways. |
| GO:1901189 positive regulation of ephrin receptor signaling pathway | IMP PMID:17336907 Notch signaling is essential for ventricular chamber develop... | KEEP AS NON CORE | Summary: Rbpj loss reduces expression of signalling components (e.g. Bmp10, Nrg1/ErbB, ephrinB2, Wnt pathway targets) in the developing heart, altering those pathways. Reason: Indirect, transcriptional cross-regulation of other pathways via Notch targets; not a direct role of Rbpj in those signalling pathways. |
| GO:1990433 CSL-Notch-Mastermind transcription factor complex | IEA GO_REF:0000107 | ACCEPT | Summary: CSL-NICD-Mastermind ternary transcription complex. Reason: Rbpj is the DNA-binding core of this complex. Supporting Evidence: PMID:18381292 we show that the addition of a RAM peptide in trans facilitates formation of the CSL-NotchIC-Mastermind ternary complex in vitro. |
| GO:1990433 CSL-Notch-Mastermind transcription factor complex | ISO GO_REF:0000119 | ACCEPT | Summary: CSL-NICD-Mastermind ternary transcription complex. Reason: Rbpj is the DNA-binding core of this complex. Supporting Evidence: PMID:18381292 we show that the addition of a RAM peptide in trans facilitates formation of the CSL-NotchIC-Mastermind ternary complex in vitro. |
| GO:2000138 positive regulation of cell proliferation involved in heart morphogenesis | IMP PMID:17336907 Notch signaling is essential for ventricular chamber develop... | KEEP AS NON CORE | Summary: Cardiovascular developmental phenotype of Rbpj loss-of-function (conventional or conditional) in mouse, reflecting loss of canonical Notch signalling in endocardium, myocardium, epicardium, valves or endothelium. Reason: Experimentally supported requirement, but a tissue-specific downstream outcome of Rbpj's core role as the Notch transcriptional effector rather than a distinct function. |
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Download this section (compressed HTML)Q: To what extent do Notch-independent Rbpj functions (PTF1-J complex in pancreas and neural tube) contribute to the pleiotropic Rbpj knockout phenotypes currently annotated as Notch pathway outputs?
Experiment: Compare phenotypes of an Rbpj allele selectively defective in NICD binding with one defective in Ptf1a binding, to separate Notch-dependent and Notch-independent developmental roles.
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